| Interacting proteins: P29353 and P19174 |
Pubmed |
SVM Score :0.50058147 |
| We further found the association of Shc with PLCgamma 1 . 0.50058147^^^ |
|
| Interacting proteins: P29353 and P19174 |
Pubmed |
SVM Score :0.0 |
| The mutated receptor also mediated unimpaired autophosphorylation ; SHC , PLC gamma 1 , and ERK tyrosine phosphorylation ; and PI 3 kinase and ERK activation . ^^^ |
|
| Interacting proteins: P29353 and P19174 |
Pubmed |
SVM Score :0.0 |
| We show that phospholipase C gamma 1 , Ras GTPase activating protein , the p 85 subunit of phosphatidylinositol 3 ' kinase , Shc , Grb 2 , Vav , Fyn , and Src are components of the Flk2 / Flt3 signal transduction pathway . ^^^ In addition , we demonstrate that phospholipase C gamma 1 , the p 85 subunit of phosphatidylinositol 3 ' kinase , Shc , Grb 2 , and Src family tyrosine kinases , but not Ras GTPase activating protein , Vav , or Nck , physically associate with the Flk2 / Flt3 cytoplasmic domain . ^^^ |
|
| Interacting proteins: P29353 and P19174 |
Pubmed |
SVM Score :0.0 |
| In contrast to SHC , phospholipase C gamma 1 , and the p 85 alpha phosphatidylinositol 3 ' kinase subunit , the endogenous GAP product ( p120GAP ) was highly tyrosine phosphorylated only in cells transformed by wild type 5 src . ^^^ |
|
| Interacting proteins: P29353 and P19174 |
Pubmed |
SVM Score :0.0 |
| Here we have investigated the relative contribution of p140trkA and p75NGFR to early consequences of NGF binding : ligand internalization , p140trkA autophosphorylation , and tyrosine phosphorylation of Shc , phospholipase C gamma 1 ( PLC gamma 1 ) , and extracellular signal regulated kinases ( ERKs ) . ^^^ |
|
| Interacting proteins: P29353 and P19174 |
Pubmed |
SVM Score :0.0 |
| To transmit NGF signals , NGF activated Trk rapidly associates with the cytoplasmic proteins , SHC , PI 3 kinase , and PLC gamma 1 . ^^^ |
|
| Interacting proteins: P29353 and P19174 |
Pubmed |
SVM Score :0.0 |
| Maximal inhibition of VGF mRNA induction was achieved using K 252a , an inhibitor of the NGF receptor Trk tyrosine kinase , and by mutating both Y 490 ( SHC association site ) and Y 785 ( PLC gamma 1 association site ) of Trk . ^^^ |
|
| Interacting proteins: P29353 and P19174 |
Pubmed |
SVM Score :0.0 |
| METHODS : Immunodetection was used to compare the expression and tyrosine phosphorylation state of PLC gamma 1 , EGFR , phosphatidylinositol 3 kinase ( PI 3 kinase ) , ras guanosine triphosphatase activating protein ( GAP ) , and src homologous collagen like protein ( SHC ) in the postnatal rat intestine . ^^^ |
|
| Interacting proteins: P29353 and P19174 |
Pubmed |
SVM Score :0.0 |
| In contrast to EGF induced tyrosine phosphorylation of the GTPase activating protein for ras ( rasGAP ) and phospholipase C gamma 1 ( PLC gamma 1 ) , SHC tyrosine phosphorylation occurs at a very low receptor density in parental NIH3T3 mouse fibroblasts expressing less than 1 10 10 ( 4 ) EGF receptors per cell . ^^^ In transfected NIH3T3 cells expressing human EGF receptors ( approximately 4 10 10 ( 5 ) receptors per cell ) , maximal levels of SHC and PLC gamma 1 tyrosine phosphorylation occur when approximately 4 10 10 ( 4 ) receptors or more are occupied by ligand . ^^^ Also , EGF treatment of mouse keratinocytes , which represent a physiological target of EGF , express a low number of EGF receptors ( approximately 2 10 10 ( 4 ) receptors per cell ) , and stringently require EGF to grow , results in intense SHC tyrosine phosphorylation , compared to rasGAP or PLC gamma 1 . ^^^ |
|
| Interacting proteins: P29353 and P19174 |
Pubmed |
SVM Score :0.0 |
| Correlations among tyrosine phosphorylation of Shc , p72syk , PLC gamma 1 , and [ Ca2+ ] 1 flux in Fc gamma RIIA signaling . ^^^ In a mouse macrophage cell line transfected with human Fc gamma RIIA , cross linking Fc gamma RIIA led to the transient generation of inositol 1 , 4 , 5 trisphosphate ( IP 3 ) , [ Ca2+ ] 1 flux , and rapid tyrosine phosphorylation of cellular substrates , including Shc , PLC gamma 1 , and a tyrosine kinase p72syk . ^^^ In contrast , no tyrosine phosphorylation of Shc or PLC gamma 1 was detected in cells transfected with mutant receptors that failed to trigger [ Ca2+ ] 1 flux . ^^^ These results suggest that tyrosine phosphorylation of Shc and PLC gamma 1 is important for the initiation of [ Ca2+ ] 1 flux , and that activation of protein kinase C may modulate the activity of PLC gamma 1 through serine / threonine phosphorylation . . ^^^ |
|
| Interacting proteins: P29353 and P19174 |
Pubmed |
SVM Score :0.0 |
| Trk receptors use redundant signal transduction pathways involving SHC and PLC gamma 1 to mediate NGF responses . ^^^ To determine the role of SHC and other Trk substrates in NGF signaling , Trk receptors with mutations in Y 490 and Y 785 ( the PLC gamma 1 association site ) were introduced into PC12nnr5 cells . ^^^ We postulate that Trk receptors can activate Erk 1 by either SHC or PLC gamma 1 dependent signaling pathways . ^^^ |
|
| Interacting proteins: P29353 and P19174 |
Pubmed |
SVM Score :0.0 |
| In this study we examine the ability of tyrosine phosphorylated proteins from Jurkat T cells stimulated by CD 2 or T cell receptor CD 3 to interact with the src homology 2 or src homology 3 domains from eight different proteins involved in signal transduction in lymphocytes : Vav , Shc , Nck , phosphatidylinositol 3 kinase , phospholipase C gamma 1 , Ras GTPase activating protein , c Crk and Grb 2 . ^^^ |
|
| Interacting proteins: P29353 and P19174 |
Pubmed |
SVM Score :0.0 |
| Mutation of the Trk A residue Tyr 490 to phenylalanine prevents the association with Shc , while similar mutations at Tyr 751 or Tyr 785 are reported to prevent interaction of Trk A with the p 85 subunit of inositol phospholipid 3 kinase and phospholipase C gamma 1 , respectively . ^^^ |
|
| Interacting proteins: P29353 and P19174 |
Pubmed |
SVM Score :0.0 |
| But microinjection of neither the Shc SH 2 mutant nor PLC gamma 1 SH 2 had any effect . ^^^ |
|
| Interacting proteins: P29353 and P19174 |
Pubmed |
SVM Score :0.0 |
| Nerve growth factor induces a multimeric TrkA receptor complex in neuronal cells that includes Crk , SHC and PLC gamma 1 but excludes P130CAS . ^^^ Using receptor activated immunoprecipitation followed by analysis of the immune complexes by SDS PAGE and immunoblotting , we show that NGF induces the association of TrkA with c Crk 2 in a multimeric complex that also includes SHC and PLC gamma 1 . ^^^ While the tyrosine phosphorylation of TrkA , SHC and PLC gamma 1 increased with time in the presence of NGF and was inhibited by the tyrosine kinase inhibitor K252a , the state of tyrosine phosphorylation of c Crk 2 did not appear to change with NGF treatment . ^^^ |
|
| Interacting proteins: P29353 and P19174 |
Pubmed |
SVM Score :0.0 |
| The in vivo interaction with the EGFr / p185c neu heterodimer of several signal transduction proteins , including phospholipase C gamma 1 ( PLC gamma 1 ) , the p 85 subunit of phosphotidylinositol 3 kinase , the ras GTPase activating protein , SHC , NCK , p72RAF , and the tyrosine phosphatase SHPTP 2 , was measured by coimmunoprecipitation . ^^^ |
|
| Interacting proteins: P29353 and P19174 |
Pubmed |
SVM Score :0.0 |
| Coprecipitation experiments show that , while the 80 kDa ErbB 4 fragment is associated with the SH 2 containing molecules PLC gamma 1 and Shc , TPA did not induce the phosphorylation of these substrates in intact cells . ^^^ |
|
| Interacting proteins: P29353 and P19174 |
Pubmed |
SVM Score :0.0 |
| Tyrosine phosphorylated beta and gamma ITAM peptides immobilized on beads precipitated Syk , Lyn , Shc , Grb 2 , and phospholipase C gamma 1 from lysates of rat basophilic leukemia RBL 2H3 cells . ^^^ Phospholipase C gamma 1 , Shc , and Grb 2 were precipitated only by the diphosphorylated beta ITAM peptide . ^^^ In membrane binding assays , fusion proteins containing the Src homology 2 domains of phospholipase C gamma 1 , Shc , Syk , and Lyn directly bound the tyrosine phosphorylated ITAM peptides . ^^^ |
|
| Interacting proteins: P29353 and P19174 |
Pubmed |
SVM Score :0.0 |
| Signal proteins such as phosphatidylinositol 3 kinase , phospholipase C gamma 1 , Shc , and mitogen activated protein kinase seem to be involved in both TrkA and TrkB mediated signaling pathways . ^^^ |
|
| Interacting proteins: P29353 and P19174 |
Pubmed |
SVM Score :0.0 |
| Tyrosine phosphorylation of EGF receptor ( EGFR ) , Shc , and phospholipase C gamma 1 ( PLC gamma ) , and growth factor receptor binding protein 2 ( Grb 2 ) coprecipitation with EGFR and Shc were analyzed by Western blotting . ^^^ |
|
| Interacting proteins: P29353 and P19174 |
Pubmed |
SVM Score :0.0 |
| CD 38 ligation with the specific mAb IB 4 induced early and late signaling events in Jurkat T cells , as judged by the transient induction of tyrosine phosphorylation of phospholipase C gamma 1 , c Cbl , zeta associated protein ( ZAP ) 70 , Shc , extracellular signal regulated protein kinase 2 ( Erk 2 ) as mitogen activated protein ( MAP ) kinase , and increased expression of the activation Ag CD 69 . ^^^ |
|
| Interacting proteins: P29353 and P19174 |
Pubmed |
SVM Score :0.0 |
| In the nontransformed intestinal cell line IEC 6 , expression of EGFR , Src homology and collagen protein ( SHC ) , phospholipase C gamma 1 ( PLC gamma ) , and their tyrosine phosphorylation in response to EGF was assayed by immunoblot . ^^^ |
|
| Interacting proteins: P29353 and P19174 |
Pubmed |
SVM Score :0.0 |
| Thus , we examined the interactions of various SH 2 containing molecules like PLC gamma 1 , rasGAP , p 85 subunit of PI 3 kinase , Src , Fyn , Crk , Nck , Grb 2 and Shc with HEK 2 using in vitro binding assays , immunoprecipitations and yeast Two Hybrid assays . ^^^ |
|
| Interacting proteins: P29353 and P19174 |
Pubmed |
SVM Score :0.0 |
| Of the tyrosine phosphorylated proteins , the results suggest that two of them are PLCgamma 1 and adapter protein Shc . ^^^ |
|
| Interacting proteins: P29353 and P19174 |
Pubmed |
SVM Score :0.0 |
| Using immunoprecipitation , PI 3 kinase and tyrosine phosphorylated PLC gamma 1 and SHC were shown to be associated with tyrosine phosphorylated TrkB in response to both BDNF and NT 4 . ^^^ |
|
| Interacting proteins: P29353 and P19174 |
Pubmed |
SVM Score :0.0 |
| Herein , we demonstrate that acidic substitution of the activation loop tyrosines is sufficient to induce allosteric changes required for constitutive TrkA kinase activity as well as phosphorylation of TrkA signaling proteins such as Shc , PLCgamma 1 , FRS 2 and erk1 / 2 . ^^^ |
|
| Interacting proteins: P29353 and P19174 |
Pubmed |
SVM Score :0.0 |
| TrkB mutated at the Shc binding site supported survival and growth poorly relative to wild type TrkB , whereas TrkB mutated at the PLC gamma 1 binding site supported growth and survival well . ^^^ |
|
| Interacting proteins: P29353 and P19174 |
Pubmed |
SVM Score :0.0 |
| In addition , Tec and Lyn were shown to phosphorylate Dok 1 , whereas phosphorylated Dok 1 was demonstrated to bind to the SH 2 domains of several signaling molecules activated by SCF , including Abl , CrkL , SHIP , and PLCgamma 1 , but not those of Vav and Shc . ^^^ |
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| Interacting proteins: P29353 and P19174 |
Pubmed |
SVM Score :0.0 |
| In this study , we investigated the participation of Lck and ZAP 70 protein tyrosine kinases ( PTKs ) , as well as PLC gamma 1 and Shc proteins in PBMC activation by Schistosoma mansoni antigens . ^^^ |
|
| Interacting proteins: P29353 and P19174 |
Pubmed |
SVM Score :0.0 |
| From our binding studies using synthetic peptides , we conclude that 3 phosphohistidine can not replace phosphotyrosine in conferring high affinity binding to the phosphotyrosine binding domain of shc or the src homology 2 domain of phospholipase C gamma1 . . ^^^ |
|
| Interacting proteins: P29353 and P19174 |
Pubmed |
SVM Score :0.0 |
| Present studies demonstrate that EGF induces the co immunoprecipitation of phospholipase C ( PLC ) gamma 1 with the adaptor protein GRB 2 and the guanine nucleotide exchange factor Sos , but not with the adaptor protein SHC , in WB cells . ^^^ |
|
| Interacting proteins: P29353 and P19174 |
Pubmed |
SVM Score :0.0 |
| The ras and ODC transformed cells did not show constitutively elevated tyrosine phosphorylation of the phospholipase Cgamma 1 ( PLCgamma 1 ) , RasGTPase activating protein ( GAP ) , phosphotyrosine phosphatase Syp , Shc proteins , and phosphatidylinositol 3 kinase ( PI 3 K ) or activation of the MAP kinase ( Erk 1 and Erk 2 ) , p 70 S6 kinase or the Janus protein tyrosine kinase ( JAK ) and signal transducer and activator of transcription ( STAT ) protein 1 pathways . ^^^ |
|
| Interacting proteins: P29353 and P19174 |
Pubmed |
SVM Score :0.0 |
| To the tyrosine phosphorylated sites of TrkA several proteins , such as phospholipase C gamma 1 , the adaptor protein SHC , the phosphotyrosine phosphatase SH PTP 2 ( SYP ) associate via their SH 2 phosphotase SH PTP 2 domain . ^^^ |
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| Interacting proteins: P29353 and P19174 |
Pubmed |
SVM Score :0.0 |
| HGF stimulated Gab 1 association with c Met , Grb 2 , SHP 2 , PI3K , Shc , Crk isoforms and CrkL , but not with PLCgamma 1 . ^^^ |
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| Interacting proteins: P29353 and P19174 |
Pubmed |
SVM Score :0.0 |
| The results show that each of the major MT signaling connections , Shc , phosphatidylinositol 3 ' kinase , and phospholipase C gamma 1 , could contribute in an additive way . ^^^ |
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| Interacting proteins: P29353 and P19174 |
Pubmed |
SVM Score :0.0 |
| PymT , a membrane associated scaffold , recruits and activates Src family tyrosine kinases , and , once tyrosine phosphorylated , binds proteins with PTB and SH 2 domains such as ShcA , phosphatidylinositol 3 kinase ( PI3K ) and phospholipase Cgamma 1 ( PLCgamma 1 ) . ^^^ PymT variants unable to bind PI3K and PLCgamma 1 directly induced hemangiomas similarly to wild type , but a mutant unable to bind ShcA was transformation compromised . ^^^ |
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| Interacting proteins: P29353 and P19174 |
Pubmed |
SVM Score :0.0 |
| Tyrosine phosphorylation stimulates PI3K and PLCgamma 1 enzymatic activity , and on ShcA creates binding sites for Grb 2 with its associated Sos 1 and Gab 1 . ^^^ |
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| Interacting proteins: P29353 and P19174 |
Pubmed |
SVM Score :0.0 |
| Second , disruption of the interaction of the insulin receptor with PLC gamma 1 by microinjection of SH 2 domains derived from PLC gamma 1 or Grb 2 but not Shc similarly blocks insulin induced DNA synthesis . ^^^ |
|
| Interacting proteins: P29353 and P19174 |
Pubmed |
SVM Score :0.0 |
| METHODS : Sections of postnatal 5 day old and adult rat , and aged human retina , and cell cultures prepared from selected cell populations of young rat retina , were immunolabeled with specific antisera to FGFR ( FGFR 1 , 2 , 3 , and 4 ) or candidate signaling molecules [ phospholipase Cg 1 ( PLCg 1 ) , son of sevenless 1 and 2 ( SOS 1 , SOS 2 ) , extracellular signal regulated kinase 1 and 2 ( ERK1 / 2 ) , protein tyrosine phosphatase ( SH PTP 2 ) and SH 2 containing protein ( Shc ) ] , and with multiple retinal cell type specific antibodies . ^^^ |
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| Interacting proteins: P29353 and P19174 |
Pubmed |
SVM Score :0.0 |
| However , even at concentrations of 0 . 1 to 1000 microM , no significant inhibitions were observed against other SH 2 domains binding such as the growth factor receptor binding protein 2 ( Grb 2 ) SH 2 domain to phosphopeptide of Shc and phospholipase Cgamma 1 ( PLCgamma 1 ) SH 2 domain to translational elongation factor 1alpha ( EF1alpha ) C terminal . ^^^ |
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| Interacting proteins: P29353 and P19174 |
Pubmed |
SVM Score :0.0 |
| While both the AspGlu and GluAsp mutant Trks support NGF dependent transient phosphorylation of Shc , PLCgamma 1 , AKT , FRS 2 , SH2B as well as prolonged MAP kinase activation , the GluAsp mutant induces stronger NGF dependent tyrosine phosphorylation of FRS 2 and SH2B , as well as a stronger reduction in bromodeoxyuridine ( BrdU ) incorporation . ^^^ |
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| Interacting proteins: P29353 and P19174 |
Pubmed |
SVM Score :0.0 |
| By using this system , we showed that endosomal activation of PDGFR recruits various signaling proteins including Grb 2 , SHC , phospholipase C gamma 1 , and the p85alpha subunit of phosphatidylinositol 3 kinase into endosomes and forms signaling complexes with PDGFR . ^^^ |
|
| Interacting proteins: P29353 and P19174 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29353 and P19174 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29353 and P19174 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29353 and P19174 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29353 and P19174 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29353 and P19174 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29353 and P19174 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29353 and P19174 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29353 and P19174 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29353 and P19174 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29353 and P19174 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29353 and P19174 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29353 and P19174 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29353 and P19174 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29353 and P19174 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29353 and P19174 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29353 and P19174 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29353 and P19174 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29353 and P19174 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29353 and P19174 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29353 and P19174 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29353 and P19174 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29353 and P19174 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29353 and P19174 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29353 and P19174 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29353 and P19174 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29353 and P19174 |
Pubmed |
SVM Score :0.0 |
| NA |
|