| Interacting proteins: P20936 and P19174 |
Pubmed |
SVM Score :0.0 |
| Significantly , CD 45 did not influence the binding of Fyn , PLC gamma 1 , GAP and Vav to the same phosphopeptide . ^^^ |
|
| Interacting proteins: P20936 and P19174 |
Pubmed |
SVM Score :0.0 |
| SH 3 domains from both Grb 2 and PLCgamma 1 , but not RasGAP , bound directly to hSos homologues . ^^^ |
|
| Interacting proteins: P20936 and P19174 |
Pubmed |
SVM Score :0.0 |
| PDGF induced tyrosine phosphorylation of two cellular proteins , phospholipase C gamma 1 ( PLC gamma 1 ) and the GTPase activating protein of Ras ( GAP ) , was assayed in epithelial cells expressing each of the mutant receptors . ^^^ Tyrosine phosphorylation of GAP and PLC gamma 1 was reduced markedly by the F 857 mutation but not significantly by the F 751 mutation . ^^^ |
|
| Interacting proteins: P20936 and P19174 |
Pubmed |
SVM Score :0.0 |
| This domain is shared by a number of signal transduction proteins including nonreceptor tyrosine kinases such as Abl , Fps , Src , and Src family members , the transforming protein Crk , phospholipase C gamma 1 , PI 3 ( phosphatidylinositol ) kinase , and guanosine triphosphatase activating protein ( GAP ) . ^^^ |
|
| Interacting proteins: P20936 and P19174 |
Pubmed |
SVM Score :0.0 |
| Binding of SH 2 domains of phospholipase C gamma 1 , GAP , and Src to activated growth factor receptors . ^^^ Phospholipase C gamma 1 ( PLC gamma 1 ) and p21ras guanosine triphosphatase ( GTPase ) activating protein ( GAP ) bind to and are phosphorylated by activated growth factor receptors . ^^^ Both PLC gamma 1 and GAP contain two adjacent copies of the noncatalytic Src homology 2 ( SH 2 ) domain . ^^^ Phospholipase C gamma 1 ( PLC gamma 1 ) and p21ras guanosine triphosphatase ( GTPase ) activating protein ( GAP ) bind to and are phosphorylated by activated growth factor receptors . ^^^ |
|
| Interacting proteins: P20936 and P19174 |
Pubmed |
SVM Score :0.0 |
| The phosphorylated C terminal tail of the PDGFR bound PLC gamma 1 , but did not associate with phosphatidylinositol ( PI ) 3 ' kinase or GTPase activating protein ( GAP ) . ^^^ |
|
| Interacting proteins: P20936 and P19174 |
Pubmed |
SVM Score :0.0 |
| In contrast to SHC , phospholipase C gamma 1 , and the p 85 alpha phosphatidylinositol 3 ' kinase subunit , the endogenous GAP product ( p120GAP ) was highly tyrosine phosphorylated only in cells transformed by wild type 5 src . ^^^ |
|
| Interacting proteins: P20936 and P19174 |
Pubmed |
SVM Score :0.0 |
| Tyrosine phosphorylation and association with phospholipase C gamma 1 of the GAP associated 62 kD protein after CD 2 stimulation of Jurkat T cell . ^^^ They also suggest a function of the p 62 GAP associated protein as a link between PLC gamma 1 and p21ras activation pathways after CD 2 activation . . ^^^ |
|
| Interacting proteins: P20936 and P19174 |
Pubmed |
SVM Score :0.0 |
| The tyrosine phosphorylated receptor associates with numerous SH 2 domain containing proteins which include phospholipase C gamma 1 ( PLC gamma ) , the GTPase activating protein of Ras ( GAP ) , the p 85 subunit of phosphatidylinositol 3 kinase ( PI3K ) , the phosphotyrosine phosphatase Syp , and several other proteins . ^^^ |
|
| Interacting proteins: P20936 and P19174 |
Pubmed |
SVM Score :0.0 |
| In Western analysis of phosphotyrosine immunoprecipitates , the hyperplastic agents PDGF and PMA increased tyrosine phosphorylation of phospholipase C gamma 1 ( PLC gamma 1 ) , GTPase activating protein ( GAP ) , and phosphatidylinositol 3 kinase ( PI 3 kinase ) , while the hypertrophic agonist thromboxane failed to tyrosine phosphorylate either of these three substrates . ^^^ Tyrosine kinase inhibition with herbimycin A ( 5 microM ) prevented agonist stimulated tyrosine phosphorylation of PLC gamma 1 , GAP , and PI 3 kinase . ^^^ These results indicate that tyrosine phosphorylation of PLC gamma 1 , GAP , and PI 3 kinase are specific responses for VSMC hyperplasia but not thromboxane stimulated hypertrophy . . ^^^ |
|
| Interacting proteins: P20936 and P19174 |
Pubmed |
SVM Score :0.0 |
| METHODS : Immunodetection was used to compare the expression and tyrosine phosphorylation state of PLC gamma 1 , EGFR , phosphatidylinositol 3 kinase ( PI 3 kinase ) , ras guanosine triphosphatase activating protein ( GAP ) , and src homologous collagen like protein ( SHC ) in the postnatal rat intestine . ^^^ |
|
| Interacting proteins: P20936 and P19174 |
Pubmed |
SVM Score :0.0 |
| Human ltk receptor tyrosine kinase binds to PLC gamma 1 , PI 3 K , GAP and Raf 1 in vivo . ^^^ Western blot analysis revealed that the wild type ltk protein was tyrosine phosphorylated in vivo and associated with SH 2 containing proteins , PLC gamma 1 , p 85 subunit of PI 3 K and GAP , in vivo . ^^^ |
|
| Interacting proteins: P20936 and P19174 |
Pubmed |
SVM Score :0.0 |
| Stimulation of RTK activity , as determined by antiphosphotyrosine immunoblot analysis of PDGF receptor and the receptor substrates phospholipase C gamma 1 ( PLC gamma 1 ) and guanosine triphosphatase activating protein ( GAP ) , was also concentration dependent . ^^^ The tyrosine kinase inhibitor genistein blocked PDGF induced RTK activity , as measured by PDGF receptor , PLC gamma 1 , and GAP tyrosine phosphorylation , in a concentration dependent manner but did not affect PDGF mediated inhibition of GJC . ^^^ These results suggest that inhibition of GJC by ligand activated PDGF receptor is dissociable from the RTK activity responsible for PDGF , PLC gamma 1 , and GAP phosphorylation . . ^^^ |
|
| Interacting proteins: P20936 and P19174 |
Pubmed |
SVM Score :0.0 |
| These kinases act directly or indirectly to phosphorylate and / or activate effector proteins including p 42 ( microtubule associated protein kinase ) ( MAPK ) , phospholipases C gamma 1 ( PLC gamma 1 ) and C gamma 2 ( PLC gamma 2 ) , phosphatidylinositol 3 kinase ( PI 3 K ) , and p21ras GTPase activating protein ( GAP ) . ^^^ |
|
| Interacting proteins: P20936 and P19174 |
Pubmed |
SVM Score :0.0 |
| In addition , the EGF induced phosphorylation of GAP , p 85 , and PLC gamma 1 did not correlate with the dominant negative action of p185K757M on EGFr function . ^^^ |
|
| Interacting proteins: P20936 and P19174 |
Pubmed |
SVM Score :0.0 |
| The p 85 N + C ( amino plus carboxyl ) SH 2 domains were demonstrated to associate with the autophosphorylated beta subunit , while neither the GTPase activator protein ( GAP ) N SH 2 domain nor the phospholipase C gamma 1 ( PLC gamma 1 ) N + C SH 2 domains exhibited measurable affinity for the activated receptor . ^^^ |
|
| Interacting proteins: P20936 and P19174 |
Pubmed |
SVM Score :0.0 |
| Immunoprecipitation experiments with specific antibodies against potential candidate proteins in the molecular mass range around 130kDa revealed positive results for the focal adhesion kinase FAK and the p 130 Src substrate while negative results were obtained for the GTPase activating protein GAP , the phospholipase C gamma 1 , the Janus kinase JAK 1 and vinculin . ^^^ |
|
| Interacting proteins: P20936 and P19174 |
Pubmed |
SVM Score :0.0 |
| Purified amino terminal Src homology 2 ( SH 2 ) domains of GAP , PLCgamma 1 and the p85alpha subunit of PI 3 kinase , as well as the carboxy terminal SH 2 domain of the latter protein and the unique SH 2 domain of Grb 2 , were injected into full grown , stage 6 Xenopus laevis oocytes . ^^^ |
|
| Interacting proteins: P20936 and P19174 |
Pubmed |
SVM Score :0.0 |
| The ras and ODC transformed cells did not show constitutively elevated tyrosine phosphorylation of the phospholipase Cgamma 1 ( PLCgamma 1 ) , RasGTPase activating protein ( GAP ) , phosphotyrosine phosphatase Syp , Shc proteins , and phosphatidylinositol 3 kinase ( PI 3 K ) or activation of the MAP kinase ( Erk 1 and Erk 2 ) , p 70 S6 kinase or the Janus protein tyrosine kinase ( JAK ) and signal transducer and activator of transcription ( STAT ) protein 1 pathways . ^^^ |
|
| Interacting proteins: P20936 and P19174 |
Pubmed |
SVM Score :0.0 |
| Autophosphorylation of the platelet derived growth factor ( PDGF ) receptor triggers intracellular signaling cascades as a result of recruitment of Src homology 2 domain containing enzymes , including phosphatidylinositol 3 kinase ( PI3K ) , the GTPase activating protein of Ras ( GAP ) , the protein tyrosine phosphatase SHP 2 , and phospholipase C gamma 1 ( PLC gamma 1 ) , to specific phosphotyrosine residues . ^^^ These mutants included a kinase deficient receptor and receptors in which various combinations of the tyrosine residues required for the binding of PI3K ( Tyr ( 740 ) and Tyr ( 751 ) ) , GAP ( Tyr ( 771 ) ) , SHP 2 ( Tyr ( 1009 ) ) , or PLC gamma 1 ( Tyr ( 1021 ) ) were mutated to Phe . ^^^ In contrast , PDGF induced H ( 2 ) O ( 2 ) production in cells expressing a receptor in which the binding sites for GAP , SHP 2 , and PLC gamma 1 were all mutated was slightly greater than that in cells expressing the wild type receptor . ^^^ |
|
| Interacting proteins: P20936 and P19174 |
Pubmed |
SVM Score :0.0 |
| Presence of SH 2 domains of phospholipase C gamma 1 enhances substrate phosphorylation by increasing the affinity toward the epidermal growth factor receptor . src homology region 2 and 3 ( SH 2 and SH 3 ) domains are conserved noncatalytic regions originally described in cytoplasmic tyrosine kinases and subsequently identified in phospholipase C gamma 1 ( PLC gamma 1 ) , GTPase activating protein of ras , and other signaling proteins . ^^^ |
|
| Interacting proteins: P20936 and P19174 |
Pubmed |
SVM Score :0.0 |
| Role of rap1B and p21ras GTPase activating protein in the regulation of phospholipase C gamma 1 in human platelets . ^^^ Using specific antibodies , we found that phospholipase C gamma 1 and the p21ras GTPase activating protein , rasGAP , are present in human platelets . ^^^ |
|
| Interacting proteins: P20936 and P19174 |
Pubmed |
SVM Score :0.0 |
| The binding of cytoplasmic signaling proteins such as phospholipase C gamma 1 and Ras GTPase activating protein to autophosphorylated growth factor receptors is directed by their noncatalytic Src homology region 2 ( SH 2 ) domains . ^^^ |
|
| Interacting proteins: P20936 and P19174 |
Pubmed |
SVM Score :0.0 |
| Combinations of immunoprecipitation and immunoblotting with antibodies specific for proteins known to be associated with signaling pathways demonstrated that none of the major tyrosine phosphorylated species correlated with phospholipase C gamma 1 , GTPase activating protein , or phosphatidylinositol 3 ' kinase . ^^^ |
|
| Interacting proteins: P20936 and P19174 |
Pubmed |
SVM Score :0.0 |
| Furthermore , the NGF dependent pp 38 SH binding is specific for the SH 2 domains of PLC gamma 1 , since the phosphoprotein does not bind to fusion proteins containing SH domains of ras GTPase activating protein or the p 85 subunit of phosphatidylinositol 3 kinase . ^^^ |
|
| Interacting proteins: P20936 and P19174 |
Pubmed |
SVM Score :0.0 |
| Src homology 2 domains bind to tyrosine phosphorylated growth factor receptors and are found in proteins that serve as substrates for tyrosine kinases , such as phospholipase C gamma 1 and ras GTPase activating protein . ^^^ |
|
| Interacting proteins: P20936 and P19174 |
Pubmed |
SVM Score :0.0 |
| In normal mast cells , Steel induces Kit to autophosphorylate on tyrosine and bind to phosphatidylinositol 3 ' kinase ( PI3K ) and phospholipase C gamma 1 but not detectably to Ras GTPase activating protein . ^^^ |
|
| Interacting proteins: P20936 and P19174 |
Pubmed |
SVM Score :0.0 |
| WT EGF receptor from EGF treated cells bound well to bacterially expressed src homology ( SH ) regions of PLC gamma 1 and to a lesser extent to bacterially expressed GTPase activating protein SH regions . ^^^ |
|
| Interacting proteins: P20936 and P19174 |
Pubmed |
SVM Score :0.0 |
| We show that phospholipase C gamma 1 , Ras GTPase activating protein , the p 85 subunit of phosphatidylinositol 3 ' kinase , Shc , Grb 2 , Vav , Fyn , and Src are components of the Flk2 / Flt3 signal transduction pathway . ^^^ In addition , we demonstrate that phospholipase C gamma 1 , the p 85 subunit of phosphatidylinositol 3 ' kinase , Shc , Grb 2 , and Src family tyrosine kinases , but not Ras GTPase activating protein , Vav , or Nck , physically associate with the Flk2 / Flt3 cytoplasmic domain . ^^^ |
|
| Interacting proteins: P20936 and P19174 |
Pubmed |
SVM Score :0.0 |
| On the other hand , a mutant PDGF receptor that was able to bind PLC gamma 1 but not other signaling proteins ( including the Ras GTPase activating protein , phosphatidylinositol 3 kinase , and a SH 2 containing phosphotyrosine phosphatase ( Syp ) ) restored the stimulatory effect of PDGF on PLC and PLD . ^^^ |
|
| Interacting proteins: P20936 and P19174 |
Pubmed |
SVM Score :0.0 |
| In contrast to EGF induced tyrosine phosphorylation of the GTPase activating protein for ras ( rasGAP ) and phospholipase C gamma 1 ( PLC gamma 1 ) , SHC tyrosine phosphorylation occurs at a very low receptor density in parental NIH3T3 mouse fibroblasts expressing less than 1 10 10 ( 4 ) EGF receptors per cell . ^^^ |
|
| Interacting proteins: P20936 and P19174 |
Pubmed |
SVM Score :0.0 |
| Cytosol from EGF treated A 431 human epidermoid carcinoma cells was used as a source of PTPase activity , and tyrosine phosphorylated ErbB 2 , EGF receptor , phospholipase C gamma 1 , and the Ras GTPase activating protein were used as substrates to monitor PTPase activity . ^^^ EGF stimulated PTPase activity that was selective toward these substrates , as it dephosphorylated ErbB 2 and the EGF receptor , but not phospholipase C gamma 1 and the Ras GTPase activating protein . ^^^ |
|
| Interacting proteins: P20936 and P19174 |
Pubmed |
SVM Score :0.0 |
| Receptor association , as measured by coimmunoprecipitation , has been determined for phospholipase C gamma 1 , the ras GTPase activating protein , the p 85 subunit of phosphatidylinositol 3 kinase , and the src homology and collagen protein . ^^^ |
|
| Interacting proteins: P20936 and P19174 |
Pubmed |
SVM Score :0.0 |
| Finally , PDGF dependent tyrosine phosphorylation of phospholipase C gamma 1 and GTPase activating protein was reduced by 60 70 % in the PTPase+ cells as was tyrosine phosphorylation of the PDGF receptor associated with these proteins . ^^^ |
|
| Interacting proteins: P20936 and P19174 |
Pubmed |
SVM Score :0.0 |
| Potential molecules implicated in downstream signaling pathways of p185BCR ABL in Ph+ ALL involve GTPase activating protein , phospholipase C gamma 1 , and phosphatidylinositol 3 ' kinase . ^^^ |
|
| Interacting proteins: P20936 and P19174 |
Pubmed |
SVM Score :0.0 |
| Several proteins were detected in normal IEC 6 cells , including GTPase activating protein , raf 1 kinase , phospholipase C gamma 1 , and phosphoinositide 3 kinase . ^^^ |
|
| Interacting proteins: P20936 and P19174 |
Pubmed |
SVM Score :0.0 |
| In the F 5 mutant lacking binding sites for phosphatidylinositol 3 kinase , GTPase activating protein , SHP 2 , and phospholipase Cgamma 1 ( PLCgamma 1 ) , PDGF induced mitogen activated protein kinase , but failed to affect GJC or Cx 43 , indicating involvement of additional signals presumably initiated by one or more of the mutated binding sites . ^^^ |
|
| Interacting proteins: P20936 and P19174 |
Pubmed |
SVM Score :0.0 |
| In addition , we demonstrate that TCR activation of normal CD4+ T cells induced the formation of p120GAP and PLCgamma 1 containing complexes . ^^^ |
|
| Interacting proteins: P20936 and P19174 |
Pubmed |
SVM Score :0.0 |
| We describe herein that Sam 68 associates with p120GAP and PLC gamma 1 in human mature T cells and in a T cell line expressing the CD 4 molecule HUT 78 CD4+ . ^^^ We propose that recruitment of Sam 68 , via CD4 / p56lck , to the inner face of the plasma membrane may permit , via its docking properties , the correct association of key signaling molecules including PLC gamma 1 and p120GAP . ^^^ |
|
| Interacting proteins: P20936 and P19174 |
Pubmed |
SVM Score :0.0 |
| We have correlated these inhibitions with the disruption of multifunctional complexes containing PLCgamma 1 , p120GAP and Sam 68 , induced by T cell activation . ^^^ |
|
| Interacting proteins: P20936 and P19174 |
Pubmed |
SVM Score :0.0 |
| The discovery that rasGAP , PI 3 kinase and PLC gamma 1 are substrates for the EGF receptor tyrosine kinase has provided a beginning in understanding the biochemistry underlying growth factor receptor transduction . . ^^^ |
|
| Interacting proteins: P20936 and P19174 |
Pubmed |
SVM Score :0.0 |
| Other GST fusion proteins containing the SH 2 domain of p 85 of phosphatidylinositol 3 kinase , phospholipase C gamma 1 , and ras GAP also precipitated c Kit . ^^^ |
|
| Interacting proteins: P20936 and P19174 |
Pubmed |
SVM Score :0.0 |
| Upon ligand induced tyrosine phosphorylation , the platelet derived growth factor ( PDGF ) receptor ( PDGFR ) beta subunit associates with PLC gamma 1 , RasGAP , P13K , and a 64 kd protein . ^^^ |
|
| Interacting proteins: P20936 and P19174 |
Pubmed |
SVM Score :0.0 |
| CONCLUSIONS : PC 12 cells expressing the beta PDGF receptor extend neurites in response to PDGF in the absence of signalling through PI 3 kinase , RasGAP , and PLC gamma 1 . ^^^ |
|
| Interacting proteins: P20936 and P19174 |
Pubmed |
SVM Score :0.0 |
| During mitosis , Sam 68 undergoes tyrosine phosphorylation , which negatively regulates its nucleic acid binding properties and mediates the interaction of Sam 68 with critical SH 2 containing signaling proteins such as Grb 2 , PLC gamma 1 and Ras GAP . ^^^ |
|
| Interacting proteins: P20936 and P19174 |
Pubmed |
SVM Score :0.0 |
| Association of PDGFR with PLC gamma 1 and RasGAP followed by their tyrosine phosphorylation failed , however , to activate PLC gamma 1 , protein kinase C ( PKC ) , and Ras . ^^^ |
|
| Interacting proteins: P20936 and P19174 |
Pubmed |
SVM Score :0.0 |
| Thus , we examined the interactions of various SH 2 containing molecules like PLC gamma 1 , rasGAP , p 85 subunit of PI 3 kinase , Src , Fyn , Crk , Nck , Grb 2 and Shc with HEK 2 using in vitro binding assays , immunoprecipitations and yeast Two Hybrid assays . ^^^ |
|
| Interacting proteins: P20936 and P19174 |
Pubmed |
SVM Score :0.0 |
| SLM 1 bound the SH 2 and SH 3 domains of p 59 ( fyn ) , Grb 2 , phospholipase Cgamma 1 ( PLCgamma 1 ) , and / or p 120 ( rasGAP ) , suggesting it may function as a multifunctional adapter protein for Src during mitosis . ^^^ Moreover , SLM 2 did not associate with the SH 3 domains of p 59 ( fyn ) , Grb 2 , PLCgamma 1 , or p 120 ( rasGAP ) , suggesting that SLM 2 may not function as an adapter protein for these proteins . ^^^ |
|
| Interacting proteins: P20936 and P19174 |
Pubmed |
SVM Score :0.0 |
| Expression of a partially activated A Raf mutant resulted in decreased tyrosine phosphorylation of the PDGFR , specifically on Y 857 ( autophosphorylation site ) and Y 1021 ( phospholipase Cgamma 1 ( PLCgamma 1 ) binding site ) , but not the binding sites for other signalling proteins ( Nck , phosphatidylinositol 3 ' kinase ( PI3K ) , RasGAP , Grb 2 , SHP ) . ^^^ |
|
| Interacting proteins: P20936 and P19174 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P20936 and P19174 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P20936 and P19174 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P20936 and P19174 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P20936 and P19174 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P20936 and P19174 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P20936 and P19174 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P20936 and P19174 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P20936 and P19174 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P20936 and P19174 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P20936 and P19174 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P20936 and P19174 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P20936 and P19174 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P20936 and P19174 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P20936 and P19174 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P20936 and P19174 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P20936 and P19174 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P20936 and P19174 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P20936 and P19174 |
Pubmed |
SVM Score :0.0 |
| NA |
|