| Interacting proteins: P49916 and P18887 |
Pubmed |
SVM Score :0.90645523 |
| XRCC 1 His directly interacted with human DNA ligase 3 in vitro to form a complex that was resistant to 2 M NaCl . 0.90645523^^^ XRCC 1 His interacted equally well with DNA ligase 3 from Bloom syndrome , HeLa and MRC 5 cells , indicating that Bloom syndrome DNA ligase 3 is normal in this respect . 0.60256789^^^ |
|
| Interacting proteins: P49916 and P18887 |
Pubmed |
SVM Score :0.81233707 |
| Subcloned fragments of XRCC 1 have been expressed and assayed for their ability to associate with DNA ligase 3 by far Western and affinity precipitation analyses . 0.81233707^^^ The C terminal 96 amino acids of XRCC 1 are necessary and sufficient for the specific interaction with DNA ligase 3 . 0.64840609^^^ Human DNA ligase 3 ( 103 kDa ) has been shown to interact directly with the 70 kDa DNA repair protein , XRCC 1 . 0.53825235^^^ |
|
| Interacting proteins: P49916 and P18887 |
Pubmed |
SVM Score :0.58094224 |
| The interaction between DNA ligase 3 alpha and XRCC 1 , which occurs through BRCT motifs in the C termini of these polypeptides , implicates this isoform of DNA ligase 3 in the repair of DNA single strand breaks and BER . 0.58094224^^^ |
|
| Interacting proteins: P49916 and P18887 |
Pubmed |
SVM Score :1.0537056 |
| These two regions of XRCC 1 have been shown to interact independently with DNA ligase 3 and poly ( ADP ribose ) polymerase as part of a mechanism involved in the repair of DNA single strand breaks . 1.0537056^^^ |
|
| Interacting proteins: P49916 and P18887 |
Pubmed |
SVM Score :0.75174164 |
| In somatic cells , DNA ligase 3 alpha forms a stable complex with the DNA repair protein Xrcc 1 . 0.75174164^^^ |
|
| Interacting proteins: P49916 and P18887 |
Pubmed |
SVM Score :0.0 |
| Furthermore , the AMP binding capacity of DNA ligase 3 and its enzymatic activity with the synthetic polymer were restored after transfection of EM 9 with the human XRCC 1 gene . ^^^ Immunoblotting data suggest that the XRCC 1 gene does not code for DNA ligase 3 . ^^^ In conclusion , the data indicate that the EM 9 cell strain has an altered DNA ligase 3 activity that can be restored by the XRCC 1 gene product . . ^^^ |
|
| Interacting proteins: P49916 and P18887 |
Pubmed |
SVM Score :0.0 |
| The 103 kDa product of one cDNA clone formed a characteristic complex with the XRCC 1 DNA repair protein and was identical with the previously described DNA ligase 3 . ^^^ |
|
| Interacting proteins: P49916 and P18887 |
Pubmed |
SVM Score :0.0 |
| Human DNA repair gene XRCC 1 complements the strand break rejoining defect in Chinese hamster mutant EM 9 and encodes a protein that is apparently required for optimal activity of DNA ligase 3 . ^^^ |
|
| Interacting proteins: P49916 and P18887 |
Pubmed |
SVM Score :0.0 |
| An interaction between the mammalian DNA repair protein XRCC 1 and DNA ligase 3 . ^^^ Affinity chromatography of extract from EM 9 cells transfected with pcD2EHX resulted in the copurification of histidine tagged XRCC 1 and DNA ligase 3 activity . ^^^ Neither XRCC 1 or DNA ligase 3 activity was purified during affinity chromatography of extract from EM 9 cells transfected with pcD2EX , a cDNA minigene that encodes untagged XRCC 1 , or extract from wild type AA 8 or untransfected EM 9 cells . ^^^ The copurification of DNA ligase 3 activity with histidine tagged XRCC 1 suggests that the two proteins are present in the cell as a complex . ^^^ These findings indicate that XRCC 1 is required for normal levels of DNA ligase 3 activity , and they implicate a major role for this DNA ligase in DNA base excision repair in mammalian cells . . ^^^ |
|
| Interacting proteins: P49916 and P18887 |
Pubmed |
SVM Score :0.0 |
| The XRCC 1 protein , which is known to bind DNA ligase 3 , is not absolutely required for the reaction but suppresses strand displacement by DNA polymerase beta , allowing for more efficient ligation after filling of a single nucleotide patch . ^^^ The region of interaction with DNA polymerase beta is located within residues 84 183 in the N terminal half of the XRCC 1 protein , whereas the C terminal region of XRCC 1 is involved in binding DNA ligase 3 . ^^^ |
|
| Interacting proteins: P49916 and P18887 |
Pubmed |
SVM Score :0.0 |
| As reported previously , the 103 kDa DNA ligase 3 alpha interacts with the DNA strand break repair protein encoded by the human XRCC 1 gene . ^^^ In contrast , the 96 kDa DNA ligase 3 beta does not interact with XRCC 1 , indicating that DNA ligase 3 beta may play a role in cellular functions distinct from the DNA repair pathways involving the DNA ligase 3 alpha 10 XRCC 1 complex . ^^^ |
|
| Interacting proteins: P49916 and P18887 |
Pubmed |
SVM Score :0.0 |
| Involvement of XRCC 1 and DNA ligase 3 gene products in DNA base excision repair . ^^^ DNA ligase 3 and the essential protein XRCC 1 are present at greatly reduced levels in the xrcc 1 mutant CHO cell line EM C 11 . ^^^ Full complementation of the ligation defect in EM C 11 extracts was achieved by addition to the repair reaction of recombinant human DNA ligase 3 but not by XRCC 1 . ^^^ This is consistent with the notion that XRCC 1 acts as an important stabilizing factor of DNA ligase 3 . ^^^ |
|
| Interacting proteins: P49916 and P18887 |
Pubmed |
SVM Score :0.0 |
| The ubiqitously expressed DNA ligase 3 alpha forms a complex with the DNA single strand break repair protein XRCC 1 . ^^^ In contrast , DNA ligase 3 beta , which does not interact with XRCC 1 , is only expressed in male meiotic germ cells , suggesting a role for this isoform in meiotic recombination . ^^^ |
|
| Interacting proteins: P49916 and P18887 |
Pubmed |
SVM Score :0.0 |
| Given that XRCC 1 is also associated with DNA ligase 3 via a second BRCT module and with DNA polymerase beta , our results provide strong evidence that PARP is a member of a BER multiprotein complex involved in the detection of DNA interruptions and possibly in the recruitment of XRCC 1 and its partners for efficient processing of these breaks in a coordinated manner . ^^^ |
|
| Interacting proteins: P49916 and P18887 |
Pubmed |
SVM Score :0.0 |
| XRCC 1 can interact with DNA polymerase beta , thereby suppressing strand displacement , and DNA ligase 3 , both of which have been implicated in base excision repair . ^^^ |
|
| Interacting proteins: P49916 and P18887 |
Pubmed |
SVM Score :0.0 |
| XRCC 1 polypeptide interacts with DNA polymerase beta and possibly poly ( ADP ribose ) polymerase , and DNA ligase 3 is a novel molecular ' nick sensor ' in vitro . ^^^ The DNA repair proteins XRCC 1 and DNA ligase 3 are physically associated in human cells and directly interact in vitro and in vivo . ^^^ |
|
| Interacting proteins: P49916 and P18887 |
Pubmed |
SVM Score :0.0 |
| We report here that XRCC 1 dependent strand break repair in G ( 1 ) phase of the cell cycle is abolished by mutations created within the XRCC 1 BRCT domain that interact with DNA ligase 3 . ^^^ These data describe a cell cycle specific role for a BRCT domain , and we conclude that the XRCC 1 DNA ligase 3 complex is required for DNA strand break repair in G ( 1 ) phase of the cell cycle but is dispensable for this process in S phase . ^^^ |
|
| Interacting proteins: P49916 and P18887 |
Pubmed |
SVM Score :0.0 |
| XRCC 1 ( 10 ray cross complementing group 1 ) is a DNA repair protein that forms complexes with DNA polymerase beta ( beta Pol ) , DNA ligase 3 and poly ADP ribose polymerase in the repair of DNA single strand breaks . ^^^ |
|
| Interacting proteins: P49916 and P18887 |
Pubmed |
SVM Score :0.0 |
| The 10 ray repair cross complementing group 1 ( XRCC 1 ) protein plays a central role in the DNA base excision repair pathway by interacting with DNA ligase 3 and DNA polymerase beta . ^^^ |
|
| Interacting proteins: P49916 and P18887 |
Pubmed |
SVM Score :0.0 |
| Using a combination of alanine scanning , polymer blot analysis , and photoaffinity labeling , we have identified poly ( ADP ribose ) binding sites in the following proteins : p 53 , p 21 ( CIP1 / WAF1 ) , xeroderma pigmentosum group A complementing protein , MSH 6 , DNA ligase 3 , XRCC 1 , DNA polymerase epsilon , DNA PK ( CS ) , Ku 70 , NF kappaB , inducible nitric oxide synthase , caspase activated DNase , and telomerase . ^^^ |
|
| Interacting proteins: P49916 and P18887 |
Pubmed |
SVM Score :0.0 |
| The CHO XRCC 1 mutant , EM 9 , deficient in DNA ligase 3 activity , exhibits hypersensitivity to camptothecin independent of DNA replication . ^^^ Recently , EM 9 were complemented the DNA ligase 3 interactive protein , XRCC 1 . ^^^ Defective XRCC 1 apparently accounts for the low DNA ligase 3 activity that may explain the single strand break repair deficiency of EM 9 cells . ^^^ These results suggest that EM 9 cells are sensitized to camptothecin by a mechanism that is independent of DNA replication and may be a consequence of the XRCC 1 mutation or the associated deficiency in DNA ligase 3 activity . ^^^ |
|
| Interacting proteins: P49916 and P18887 |
Pubmed |
SVM Score :0.0 |
| Furthermore , the established structure of an XRCC 1 BRCT homodimer suggests potential protein protein interaction sites for the complementary BRCT domain in DNA ligase 3 , since these two domains form a stable heterodimeric complex . ^^^ |
|
| Interacting proteins: P49916 and P18887 |
Pubmed |
SVM Score :0.0 |
| The other proteins involved in base excision repair , polymerase beta , XRCC 1 , and DNA ligase 3 , do not affect the dissociation of thymine DNA glycosylase from the apurinic site . . ^^^ |
|
| Interacting proteins: P49916 and P18887 |
Pubmed |
SVM Score :0.0 |
| Hamster EM 9 and EM C 11 cells were deficient in DNA ligase 3 activity as a consequence of mutations in the XRCC 1 gene . ^^^ |
|
| Interacting proteins: P49916 and P18887 |
Pubmed |
SVM Score :0.0 |
| The 10 ray repair cross complementing group 1 ( XRCC 1 ) protein plays a central role in the DNA base excision repair pathway by interacting with DNA ligase 3 and DNA polymerase beta . ^^^ |
|
| Interacting proteins: P49916 and P18887 |
Pubmed |
SVM Score :0.0 |
| Mitochondrial DNA ligase 3 function is independent of Xrcc 1 . ^^^ Hamster EM 9 cells , which lack Xrcc 1 protein , have reduced levels of DNA ligase 3 and are defective in nuclear base excision repair . ^^^ The Xrcc 1 protein stabilizes DNA ligase 3 and may even play a direct role in catalyzing base excision repair . ^^^ Since DNA ligase 3 is also thought to function in mitochondrial base excision repair , it seemed likely that mitochondrial DNA ligase 3 function would also be dependent upon Xrcc 1 . ^^^ Thus , mitochondrial DNA ligase 3 function is independent of the Xrcc 1 protein . . ^^^ |
|
| Interacting proteins: P49916 and P18887 |
Pubmed |
SVM Score :0.0 |
| The DNA single strand break repair protein XRCC 1 contains a BRCT domain that binds and stabilizes intracellular DNA ligase 3 protein . ^^^ Mutations that disrupt the BRCT domain and prevent DNA ligase 3 interaction abolished XRCC 1 dependent repair in serum starved Chinese hamster ovary cells , and reentry into cell cycle induced by readdition of serum restored repair . ^^^ Elevating DNA ligase 3 levels in XRCC 1 mutant cells using proteosome inhibitors or by expressing XRCC 1 protein in which the BRCT domain is disrupted but can still bind DNA ligase 3 failed to restore repair in noncycling cells . ^^^ |
|
| Interacting proteins: P49916 and P18887 |
Pubmed |
SVM Score :0.0 |
| Here , we report that XRCC 1 interacts with human polynucleotide kinase in addition to its established interactions with DNA polymerase beta and DNA ligase 3 . ^^^ |
|
| Interacting proteins: P49916 and P18887 |
Pubmed |
SVM Score :0.0 |
| BRCT domain interactions in the heterodimeric DNA repair protein XRCC 1 DNA ligase 3 . ^^^ The heterodimer of the DNA repair proteins , XRCC 1 and DNA ligase 3 , was the first example of a functional interaction via BRCT modules . ^^^ Key amino acid residues mediating the interaction with DNA ligase 3 were identified here by targeted mutagenesis of the XRCC 1 BRCT domain . ^^^ A structural model of the DNA ligase 3 BRCT domain was constructed and similarly tested by mutation of corresponding residues required for the interaction with XRCC 1 . ^^^ These data identify the XRCC 1 DNA ligase 3 heterodimer interface and provide the first demonstration of the surface contacts coordinating a functional BRCT BRCT protein interaction . . ^^^ |
|
| Interacting proteins: P49916 and P18887 |
Pubmed |
SVM Score :0.0 |
| X ray repair cross complementing gene 1 protein plays an important role in camptothecin resistance . 10 ray repair cross complementing gene 1 protein ( XRCC 1 ) in complex with DNA polymerase beta , DNA ligase 3 , and poly ( ADP ribose ) polymerase is important in the base excision repair process . ^^^ In this study , we found the expression levels of XRCC 1 protein in KB 100 and KB 300 were > or =5 fold more than in their respective revertant cell lines , whereas there was no difference in the expression of XRCC 1 associated proteins such as DNA polymerase beta , DNA ligase 3 , poly ( ADP ribose ) polymerase , and apurinic / apyrimidinic endonuclease . ^^^ |
|
| Interacting proteins: P49916 and P18887 |
Pubmed |
SVM Score :0.0 |
| PARP 2 was also found to interact with three other proteins involved in the base excision repair pathway : 10 ray cross complementing factor 1 ( XRCC 1 ) , DNA polymerase beta , and DNA ligase 3 , already known as partners of PARP 1 . ^^^ |
|
| Interacting proteins: P49916 and P18887 |
Pubmed |
SVM Score :0.0 |
| Interestingly the amino terminus of the long form aprataxin is homologous with polynucleotidekinase 3 ' phosphatase , which has been demonstrated to be involved in base excision repair , a subtype of single strand DNA break repair , through interaction with XRCC 1 , DNA polymerase beta , and DNA ligase 3 . ^^^ |
|
| Interacting proteins: P49916 and P18887 |
Pubmed |
SVM Score :0.0 |
| Polymorphisms in DNA repair gene XRCC 1 and increased genetic susceptibility to breast cancer . 10 ray repair cross complementing 1 ( XRCC 1 ) gene encodes for a scaffolding protein , which plays an important role in base excision DNA repair by bringing together DNA polymerase beta , DNA ligase 3 and poly ( ADP Ribose ) polymerase ( PARP ) at the site of DNA damage . ^^^ |
|
| Interacting proteins: P49916 and P18887 |
Pubmed |
SVM Score :0.0 |
| DNA polymerase beta promotes recruitment of DNA ligase 3 alpha XRCC 1 to sites of base excision repair . ^^^ |
|
| Interacting proteins: P49916 and P18887 |
Pubmed |
SVM Score :0.0 |
| Roles of DNA ligase 3 and XRCC 1 in regulating the switch between short patch and long patch BER . ^^^ Employing a reconstituted BER complex containing among others DNA polymerase beta ( Pol beta ) , DNA ligase 3 ( Lig 3 ) and XRCC 1 , here we show that Lig 3 and XRCC 1 are essential mediators of this regulation . ^^^ |
|
| Interacting proteins: P49916 and P18887 |
Pubmed |
SVM Score :0.0 |
| Mutational analysis and binding studies revealed that DNA Ligase 1 was recruited to DNA repair sites by interaction with PCNA while DNA Ligase 3 was recruited via its BRCT domain mediated interaction with XRCC 1 . ^^^ |
|
| Interacting proteins: P49916 and P18887 |
Pubmed |
SVM Score :0.0 |
| Aprataxin associates with the DNA repair proteins XRCC 1 and XRCC 4 , which are partners of DNA ligase 3 and ligase 4 , respectively , suggestive of a role in DNA repair . ^^^ |
|
| Interacting proteins: P49916 and P18887 |
Pubmed |
SVM Score :0.0 |
| This provides a mechanism for the recruitment of the DNA ligase IIIalpha XRCC 1 complex to in vivo DNA single strand breaks and suggests that the zinc finger of DNA ligase 3 enables this complex and associated repair factors to locate the strand break in the presence of the negatively charged poly ( ADP ribose ) polymer . . ^^^ |
|
| Interacting proteins: P49916 and P18887 |
Pubmed |
SVM Score :0.0 |
| Using a reconstituted system including the recombinant BER proteins Pol beta , AP endonuclease 1 ( APE 1 ) , 10 ray repair cross complementing group 1 ( XRCC 1 ) , DNA ligase 3 ( Lig 3 ) , flap endonuclease 1 ( FEN 1 ) , and poly ( ADP ribose ) polymerase 1 ( PARP 1 ) , it is demonstrated that in the absence of ATP , both long patch DNA synthesis by Pol beta and poly ( ADP ribosylation ) catalysed by PARP 1 are stimulated . ^^^ |
|
| Interacting proteins: P49916 and P18887 |
Pubmed |
SVM Score :0.0 |
| The greatly reduced XRCC 1 protein level destabilized the XRCC 1 partner protein DNA ligase 3 ( LIG 3 ) but still allowed for successful mouse development and healthy , fertile adults . ^^^ Thus , a large reduction of both XRCC 1 and DNA ligase 3 has no observable effect on mouse embryogenesis and post natal development , and no significant effect on cellular sensitivity to DNA alkylation . ^^^ |
|
| Interacting proteins: P49916 and P18887 |
Pubmed |
SVM Score :0.0 |
| LIG 3 mutant cells have relatively normal XRCC 1 levels but elevated sister chromatid exchange . ^^^ |
|
| Interacting proteins: P49916 and P18887 |
Pubmed |
SVM Score :0.0 |
| Selected SNPs belong to the following genes : ADH1B , ALDH 2 , APEX , CDKN2A , COMT , CYP1A1 , CYP1A2 , CYP1B1 , CYP2A6 , CYP2C19 , CYP2C9 , CYP2E1 , CYP3A4 , DRD 2 , DRD 4 , EPHX 1 , ERCC 1 , ERCC 2 , ERCC 4 , ERCC 5 , GRPR , GSTA 4 , GSTM 3 , GSTP 1 , GSTT 2 , LIG 3 , MDM 2 , MGMT , MPO , NAT 1 , NAT 2 , NQO 1 , OGG 1 , PCNA , POLB , SLC6A3 , SOD 2 , TP 53 , XRCC 1 , XRCC 2 , XRCC 3 , and XRCC 9 . ^^^ |
|
| Interacting proteins: P49916 and P18887 |
Pubmed |
SVM Score :0.0 |
| No induction at the transcriptional level of any of the base excision repair genes , NTH 1 ( NTHL 1 ) , OGG 1 , NEIL 1 , NEIL 2 , NEIL 3 , APE 1 , POLB , or accessory protein genes , LIG 3 , XRCC 1 or XPG , was found at gamma radiation doses ranging from 1 cGy to 2 Gy in a 24 h period . ^^^ |
|
| Interacting proteins: P49916 and P18887 |
Pubmed |
SVM Score :0.0 |
| In the final model , 4 SNPs , including 2 in the coding regions ( ADPRT Val762Ala and MBD 4 Glu346Lys ) and others in noncoding regions ( LIG 3 A3704G and XRCC 1 T 77C ) , remained as significant predictors for the risk of ESCC . ^^^ The adjusted odd ratios were 1 . 25 [ 95 % confidence interval ( CI ) 1 . 02 1 . 53 ] for the ADPRT 762Ala allele , 1 . 25 ( 95 % CI 1 . 02 1 . 53 ) for the MBD 4 346 Lys allele , 0 . 78 ( 95 % CI 0 . 63 0 . 97 ) for the LIG 3 3704G allele , and 1 . 38 ( 95 % CI 1 . 01 1 . 89 ) for the XRCC 1 77C allele . ^^^ |
|
| Interacting proteins: P49916 and P18887 |
Pubmed |
SVM Score :0.0 |
| Genetic polymorphisms in XRCC 1 gene could , through alteration of protein structure , lead to defective functioning of DNA Polbeta , PARP and LIG 3 enzymes resulting in defective DNA repair and increased risk of childhood acute lymphoblastic leukemia ( ALL ) . ^^^ |
|
| Interacting proteins: P49916 and P18887 |
Pubmed |
SVM Score :0.0 |
| We investigated 44 single nucleotide polymorphisms ( SNPs ) in 20 DNA repair genes including nucleotide excision repair ( NER ) genes XPA , ERCC 1 , ERCC2 / XPD , ERCC4 / XPF and ERCC5 / XPG ; base excision repair ( BER ) genes APE1 / APEX , OGG 1 , MPG , XRCC 1 , PCNA , POLB , POLiota , LIG 3 and EXO 1 ; double strand break repair ( DSB R ) genes XRCC 2 , XRCC 3 , XRCC 9 , NBS 1 and ATR ; and direct damage reversal ( DR ) gene MGMT / AGT . ^^^ |
|
| Interacting proteins: P49916 and P18887 |
Pubmed |
SVM Score :0.0 |
| We evaluated whether genetic variation in six BER pathway genes ( XRCC 1 , ADPRT , APEX 1 , OGG 1 , LIG 3 , and MUTYH ) is associated with breast cancer risk in two large population based case control studies in the United States ( 3 , 368 cases and 2 , 880 controls ) and Poland ( 1 , 995 cases and 2 , 296 controls ) . ^^^ S . study participants with mouthwash DNA , we found no significant overall association between breast cancer risk and XRCC 1 R280H and R194W , ADPRT V726W , APEX 1 D148E , OGG 1 S326C , LIG 3 R780H , or MUTYH 5 ' untranslated region . ^^^ |
|
| Interacting proteins: P49916 and P18887 |
Pubmed |
SVM Score :0.0 |
| The candidate genes belong to different DNA repair pathways : base excision repair ( OGG 1 , LIG 3 , APEX , POLB , XRCC 1 , PCNA , and MUTYH ) , nucleotide excision repair ( ERCC 1 , ERCC 2 , ERCC 4 , and ERCC 5 ) , double strand breaks repair ( XRCC 2 , XRCC 3 , and XRCC 9 ) , and reversion repair ( MGMT ) genes . ^^^ |
|
| Interacting proteins: P49916 and P18887 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P49916 and P18887 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P49916 and P18887 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P49916 and P18887 |
Pubmed |
SVM Score :0.0 |
| NA |
|