Interacting proteins: P18858 and P39748 |
Pubmed |
SVM Score :0.74769077 |
In vitro , FEN 1 can inhibit TNR expansion by employing its endonuclease activity to compete with DNA ligase 1 . 0.74769077^^^ |
|
Interacting proteins: P18858 and P39748 |
Pubmed |
SVM Score :0.0 |
Using these proteins , complete SV 40 DNA replication was reconstituted with only purified DNA replication factors : SV 40 large tumor antigen ( TAg ) , replication protein A ( RPA ) , DNA topoisomerases 1 and 2 , DNA polymerase alpha primase , replication factor C ( RFC ) , the proliferating cell nuclear antigen ( PCNA ) , DNA polymerase delta , maturation factor 1 ( MF 1 ) , and DNA ligase 1 . ^^^ MF 1 , a 5 ' to 3 ' exonuclease and DNA ligase 1 were both identified as essential components for production of covalently closed circular relaxed ( form 1 ) DNA . ^^^ |
|
Interacting proteins: P18858 and P39748 |
Pubmed |
SVM Score :0.0 |
After FEN 1 / RTH 1 action and extension by polymerization , DNA ligase 1 can join the entirely DNA strands to complete repair . . ^^^ |
|
Interacting proteins: P18858 and P39748 |
Pubmed |
SVM Score :0.0 |
By targeting loops on the surface of the PCNA trimer and changing three or four residues at a time to alanine , we found that a region including part of the domain connecting loop of PCNA and loops on one face of the trimer , close to the C termini , is involved in binding to all of the following proteins : DNA polymerase delta , replication factor C , the flap endonuclease Fen 1 , the cyclin dependent kinase inhibitor p 21 and DNA ligase 1 . ^^^ An inhibition of DNA ligation caused by the interaction of PCNA with DNA ligase 1 was found , and we show that DNA ligase 1 and Fen 1 can inhibit DNA synthesis by DNA polymerase delta / PCNA . ^^^ |
|
Interacting proteins: P18858 and P39748 |
Pubmed |
SVM Score :0.0 |
We reconstituted the final steps of long patch base excision repair in vitro using calf DNA polymerase epsilon to provide strand displacement synthesis , human flap endonuclease 1 , and human DNA ligase 1 . ^^^ |
|
Interacting proteins: P18858 and P39748 |
Pubmed |
SVM Score :0.0 |
Furthermore , several proteins , XPG , FEN 1 , and DNA ligase 1 , recently were shown to competitively bind to the same region of PCNA , the interdomain connector loop , to which DNA polymerase delta or epsilon also binds . ^^^ |
|
Interacting proteins: P18858 and P39748 |
Pubmed |
SVM Score :0.0 |
Our data indicated that efficient repair is dependent on six components including AP endonuclease , replication factor C , proliferating cell nuclear antigen , DNA polymerases delta or epsilon , flap endonuclease 1 , and DNA ligase 1 . ^^^ |
|
Interacting proteins: P18858 and P39748 |
Pubmed |
SVM Score :0.0 |
Here we have reconstituted PCNA dependent repair of AP sites with six purified human proteins : AP endonuclease , replication factor C , PCNA , flap endonuclease 1 ( FEN 1 ) , DNA polymerase delta , and DNA ligase 1 . ^^^ Disruption of the PCNA binding site of either FEN 1 or DNA ligase 1 significantly reduced efficiency of AP site repair but did not affect repair patch size . . ^^^ |
|
Interacting proteins: P18858 and P39748 |
Pubmed |
SVM Score :0.0 |
We find that transposition is elevated in cells mutated for genes in the RAD 52 recombinational repair pathway , such as RAD 50 , RAD 51 , RAD 52 , RAD 54 , or RAD 57 , or in the DNA ligase 1 gene CDC 9 , but is not elevated in cells mutated in the DNA repair functions encoded by the RAD 1 , RAD 2 , or MSH 2 genes . ^^^ |
|
Interacting proteins: P18858 and P39748 |
Pubmed |
SVM Score :0.0 |
This enhancement was linearly dependent on the amount of APE 1 added , while addition of other BER enzymes , such as DNA ligase 1 and FEN 1 , had no effect . ^^^ |
|
Interacting proteins: P18858 and P39748 |
Pubmed |
SVM Score :0.0 |
The temperature dependent lethality of dna 2 ( ts ) mutants was suppressed by overexpression of genes encoding subunits of polymerase delta ( cdc 1 ( + ) and cdc 27 ( + ) ) , DNA ligase 1 ( cdc 17 ( + ) ) , and Fen 1 ( rad 2 ( + ) ) . ^^^ |
|
Interacting proteins: P18858 and P39748 |
Pubmed |
SVM Score :0.0 |
Recently , we have reconstituted the PCNA dependent AP site repair reaction with six purified human proteins : AP endonuclease , replication factor C ( RFC ) , PCNA , flap endonuclease 1 ( FEN 1 ) , DNA polymerase delta ( pol delta ) , and DNA ligase 1 . ^^^ PCNA can directly interact with RFC , pol delta , FEN 1 and DNA ligase 1 . ^^^ |
|
Interacting proteins: P18858 and P39748 |
Pubmed |
SVM Score :0.0 |
We show here that p 21 also inhibits PCNA stimulation of long patch base excision repair ( BER ) in vitro . p 21 disrupts PCNA directed stimulation of flap endonuclease 1 ( FEN 1 ) , DNA ligase 1 , and DNA polymerase delta . ^^^ APE 1 initially cleaves the DNA and then facilitates the sequential binding and catalysis by DNA polymerase beta , DNA polymerase delta , FEN 1 , and DNA ligase 1 . ^^^ |
|
Interacting proteins: P18858 and P39748 |
Pubmed |
SVM Score :0.0 |
DNA ligase 1 competes with FEN 1 to expand repetitive DNA sequences in vitro . ^^^ Flap endonuclease 1 ( FEN 1 ) cleaved the displaced downstream strand and together with DNA ligase 1 produced non expanded products . ^^^ Flap endonuclease 1 ( FEN 1 ) cleaved the displaced downstream strand and together with DNA ligase 1 produced non expanded products . ^^^ |
|
Interacting proteins: P18858 and P39748 |
Pubmed |
SVM Score :0.0 |
The cellular proteins flap endonuclease 1 ( FEN 1 ) , proliferating cell nuclear antigen , replication factor C , DNA ligase 1 and DNA polymerase delta are required for the repair of this type of DNA lesion . ^^^ |
|
Interacting proteins: P18858 and P39748 |
Pubmed |
SVM Score :0.0 |
Specifically , APE 1 enhanced the enzymatic activity of both flap endonuclease 1 ( FEN 1 ) and DNA ligase 1 . ^^^ AP endonuclease 1 coordinates flap endonuclease 1 and DNA ligase 1 activity in long patch base excision repair . ^^^ |
|
Interacting proteins: P18858 and P39748 |
Pubmed |
SVM Score :0.0 |
Moreover , we demonstrate that both FEN 1 and human DNA ligase 1 can operate successively on DNA within the same nucleosome . ^^^ |
|
Interacting proteins: P18858 and P39748 |
Pubmed |
SVM Score :0.0 |
The PCNA heterotrimer , but not individual subunits , stimulates the activities of the DNA polymerase , DNA ligase 1 , and the flap endonuclease ( FEN 1 ) of S . solfataricus . ^^^ |
|
Interacting proteins: P18858 and P39748 |
Pubmed |
SVM Score :0.0 |
Reconstituting triplet repeat expansion in vitro , we previously found that DNA ligase 1 promotes expansion , but FEN 1 prevents the ligation that forms expanded products . ^^^ However , even wild type FEN 1 exonuclease can not compete with DNA ligase 1 to degrade a bubble structure before it can be ligated . ^^^ A model is presented describing the roles of DNA structure , DNA ligase 1 , and FEN 1 in sequence expansion . . ^^^ |
|
Interacting proteins: P18858 and P39748 |
Pubmed |
SVM Score :0.0 |
The phosphorylation of proteins such as replication protein A , DNA polymerase alpha and delta , replication factor C , flap endonuclease 1 and DNA ligase 1 leads to their inactivation , suggesting that phosphorylation is important in the prevention of re replication . ^^^ |
|
Interacting proteins: P18858 and P39748 |
Pubmed |
SVM Score :0.0 |
Nick translation by the strand displacement action of DNA polymerase 8 , coupled with the nuclease action of FEN 1 , results in processive RNA degradation until a proper DNA nick is reached for closure by DNA ligase 1 . ^^^ |
|
Interacting proteins: P18858 and P39748 |
Pubmed |
SVM Score :0.0 |
We have used a combination of in situ extraction and dual color photobleaching to compare the dynamic properties of three proteins essential for lagging strand synthesis : the polymerase clamp proliferating cell nuclear antigen ( PCNA ) and two proteins that bind to it , DNA Ligase 1 and Fen 1 . ^^^ |
|
Interacting proteins: P18858 and P39748 |
Pubmed |
SVM Score :0.0 |
The proteins and enzymatic activities that were found to copurify with the NB DNA synthesome include : DNA polymerases alpha , delta , and epsilon , proliferating cell nuclear antigen , replication factor A , replication factor C , topoisomerases 1 and 2 , flap endonuclease 1 , and DNA ligase 1 . ^^^ |
|
Interacting proteins: P18858 and P39748 |
Pubmed |
SVM Score :0.0 |
These functions include loading onto DNA by replication factor C , as well as Okazaki fragment synthesis and maturation by the PCNA coordinated actions of DNA polymerase delta , the flap endonuclease FEN 1 , and DNA ligase 1 . ^^^ |
|
Interacting proteins: P18858 and P39748 |
Pubmed |
SVM Score :0.0 |
It binds the DNA glycosylase MutY homolog , and stimulates DNA polymerase beta , flap endonuclease 1 , and DNA ligase 1 . 9 1 1 resembles proliferating cell nuclear antigen ( PCNA ) , which stimulates some of these same repair enzymes , and is loaded onto DNA in a similar manner . ^^^ |
|
Interacting proteins: P18858 and P39748 |
Pubmed |
SVM Score :0.0 |
NA |
|
Interacting proteins: P18858 and P39748 |
Pubmed |
SVM Score :0.0 |
NA |
|
Interacting proteins: P18858 and P39748 |
Pubmed |
SVM Score :0.0 |
NA |
|
Interacting proteins: P18858 and P39748 |
Pubmed |
SVM Score :0.0 |
NA |
|
Interacting proteins: P18858 and P39748 |
Pubmed |
SVM Score :0.0 |
NA |
|
Interacting proteins: P18858 and P39748 |
Pubmed |
SVM Score :0.0 |
NA |
|
Interacting proteins: P18858 and P39748 |
Pubmed |
SVM Score :0.0 |
NA |
|
Interacting proteins: P18858 and P39748 |
Pubmed |
SVM Score :0.0 |
NA |
|