| Interacting proteins: P18858 and P12004 |
Pubmed |
SVM Score :0.78969379 |
| An inhibition of DNA ligation caused by the interaction of PCNA with DNA ligase 1 was found , and we show that DNA ligase 1 and Fen 1 can inhibit DNA synthesis by DNA polymerase delta / PCNA . 0.78969379^^^ |
|
| Interacting proteins: P18858 and P12004 |
Pubmed |
SVM Score :0.52266869 |
| Thus , the interaction between PCNA and DNA ligase 1 is not only critical for the subnuclear targeting of the ligase , but also for coordination of the molecular transactions that occur during lagging strand synthesis . 0.52266869^^^ |
|
| Interacting proteins: P18858 and P12004 |
Pubmed |
SVM Score :0.63842224 |
| PCNA can directly interact with RFC , pol delta , FEN 1 and DNA ligase 1 . 0.63842224^^^ |
|
| Interacting proteins: P18858 and P12004 |
Pubmed |
SVM Score :0.85481435 |
| DNA ligase 1 interacts directly with proliferating cell nuclear antigen ( PCNA ) and DNA polymerase beta ( Pol beta ) , linking this enzyme with both short patch and long patch BER . 0.85481435^^^ |
|
| Interacting proteins: P18858 and P12004 |
Pubmed |
SVM Score :0.74773384 |
| Mutational analysis and binding studies revealed that DNA Ligase 1 was recruited to DNA repair sites by interaction with PCNA while DNA Ligase 3 was recruited via its BRCT domain mediated interaction with XRCC 1 . 0.74773384^^^ |
|
| Interacting proteins: P18858 and P12004 |
Pubmed |
SVM Score :0.0 |
| Cyclin B / p34cdc2 triggers phosphorylation of DNA ligase 1 during Xenopus laevis oocyte maturation . ^^^ Immunoprecipitated oocyte DNA ligase 1 is phosphorylated and its molecular mass modified by purified cyclin B / p34cdc2 in vitro . ^^^ |
|
| Interacting proteins: P18858 and P12004 |
Pubmed |
SVM Score :0.0 |
| Complete repair synthesis was achieved by combining these factors with DNA polymerase epsilon , RFC , PCNA , and DNA ligase 1 . ^^^ |
|
| Interacting proteins: P18858 and P12004 |
Pubmed |
SVM Score :0.0 |
| A combination of DNA polymerase epsilon , PCNA , replication factor C , replication protein A , and DNA ligase 1 appears to be well suited to the task of creating nucleotide excision repair patches . . ^^^ |
|
| Interacting proteins: P18858 and P12004 |
Pubmed |
SVM Score :0.0 |
| Proteins and enzymatic activities associated with this mouse cell multiprotein form of DNA polymerase include the DNA polymerases alpha and delta , DNA primase , proliferating cell nuclear antigen ( PCNA ) , DNA ligase 1 , DNA helicase , and DNA topoisomerases 1 and 2 . ^^^ |
|
| Interacting proteins: P18858 and P12004 |
Pubmed |
SVM Score :0.0 |
| Using these proteins , complete SV 40 DNA replication was reconstituted with only purified DNA replication factors : SV 40 large tumor antigen ( TAg ) , replication protein A ( RPA ) , DNA topoisomerases 1 and 2 , DNA polymerase alpha primase , replication factor C ( RFC ) , the proliferating cell nuclear antigen ( PCNA ) , DNA polymerase delta , maturation factor 1 ( MF 1 ) , and DNA ligase 1 . ^^^ |
|
| Interacting proteins: P18858 and P12004 |
Pubmed |
SVM Score :0.0 |
| In this study , fractionation of a HeLa nuclear extract by DNA ligase 1 affinity chromatography resulted in the specific retention of a replication protein , proliferating cell nuclear antigen ( PCNA ) , by the affinity resin . ^^^ Subsequent experiments demonstrated that DNA ligase 1 and PCNA interact directly via the amino terminal 118 aa of DNA ligase 1 , the same region of DNA ligase 1 that is required for localization of this enzyme at replication foci during S phase . ^^^ An interaction between DNA ligase 1 and PCNA that is topologically linked to DNA was detected . ^^^ However , DNA ligase 1 inhibited PCNA dependent DNA synthesis by DNA pol delta . ^^^ These observations suggest that a ternary complex of DNA ligase 1 , PCNA and DNA pol delta does not form on a gapped DNA template . ^^^ |
|
| Interacting proteins: P18858 and P12004 |
Pubmed |
SVM Score :0.0 |
| The salt eluates were examined for the presence of both DNA replication proteins ( Pol alpha , delta , straightepsilon , PCNA , RFC , RFA , DNA ligase 1 , NDH 2 , Topo 1 and Topo 2 ) and cell cycle proteins ( Cyclins A , B 1 , D 1 , D 2 , D 3 , E , CDK 2 , CDK 4 , CDK 5 and p 21 ) by western blotting with specific antibodies . ^^^ |
|
| Interacting proteins: P18858 and P12004 |
Pubmed |
SVM Score :0.0 |
| This multiprotein replication complex contains DNA polymerases alpha and delta , primase , replication factor C , replication protein A , helicase , poly ( ADPribose ) polymerase , proliferating cell nuclear antigen , DNA ligase 1 , and topoisomerases 1 and 2 . ^^^ |
|
| Interacting proteins: P18858 and P12004 |
Pubmed |
SVM Score :0.0 |
| Our results suggest that the inhibition may be due to DNA ligase 1 interaction with proliferating cell nuclear antigen ( PCNA ) and not to a direct interaction with the DNA polymerase delta itself . ^^^ Contrary to DNA polymerase delta , DNA synthesis by DNA polymerase epsilon was stimulated by DNA ligase 1 in a PCNA dependent manner . ^^^ We conclude that DNA ligase 1 displays different influences on the two multipotent DNA polymerases delta and epsilon through PCNA . ^^^ |
|
| Interacting proteins: P18858 and P12004 |
Pubmed |
SVM Score :0.0 |
| N terminal fragments of DNA ligase 1 and the RF C large subunit that contain the RFTS both interact with proliferating cell nuclear antigen ( PCNA ) in vitro . ^^^ Moreover , the RFTS of DNA ligase 1 and of the RF C large subunit is necessary and sufficient for the interaction with PCNA . ^^^ Both subnuclear targeting and PCNA binding by the DNA ligase 1 RFTS are abolished by replacement of the adjacent phenylalanine residues within box 1 . ^^^ DNA ligase 1 is recruited to sites of DNA replication by an interaction with proliferating cell nuclear antigen : identification of a common targeting mechanism for the assembly of replication factories . ^^^ |
|
| Interacting proteins: P18858 and P12004 |
Pubmed |
SVM Score :0.0 |
| The latter pathway shares some enzymatic components with the nucleotide excision repair ( NER ) pathway acting on damage induced by ultraviolet light : both pathways are strictly dependent on PCNA and several observations suggest that the polymerization and ligation phases may be carried out by common enzymatic activities ( DNA polymerase delta / epsilon and DNA ligase 1 ) . ^^^ |
|
| Interacting proteins: P18858 and P12004 |
Pubmed |
SVM Score :0.0 |
| Using proliferating cell nuclear antigen affinity chroma tography and glycerol gradient centrifugation of partially purified fractions from mouse FM3A cells we have been able to isolate novel complexes of DNA polymerase delta and DNA ligase 1 containing clearly defined subunit compositions . ^^^ |
|
| Interacting proteins: P18858 and P12004 |
Pubmed |
SVM Score :0.0 |
| Furthermore , several proteins , XPG , FEN 1 , and DNA ligase 1 , recently were shown to competitively bind to the same region of PCNA , the interdomain connector loop , to which DNA polymerase delta or epsilon also binds . ^^^ |
|
| Interacting proteins: P18858 and P12004 |
Pubmed |
SVM Score :0.0 |
| The replication factory targeting sequence / PCNA binding site is required in G ( 1 ) to control the phosphorylation status of DNA ligase 1 . ^^^ The recruitment of DNA ligase 1 to replication foci in S phase depends on a replication factory targeting sequence that also mediates the interaction with proliferating cell nuclear antigen ( PCNA ) in vitro . ^^^ The analysis of epitope tagged DNA ligase 1 mutants demonstrates that dephosphorylation of Ser 66 requires both the nuclear localization and the PCNA binding site of the enzyme . ^^^ Finally , we show that DNA ligase 1 and PCNA interact in vivo in G ( 1 ) and S phase but not in G ( 2 ) / M . ^^^ |
|
| Interacting proteins: P18858 and P12004 |
Pubmed |
SVM Score :0.0 |
| Our data indicated that efficient repair is dependent on six components including AP endonuclease , replication factor C , proliferating cell nuclear antigen , DNA polymerases delta or epsilon , flap endonuclease 1 , and DNA ligase 1 . ^^^ |
|
| Interacting proteins: P18858 and P12004 |
Pubmed |
SVM Score :0.0 |
| Here we have reconstituted PCNA dependent repair of AP sites with six purified human proteins : AP endonuclease , replication factor C , PCNA , flap endonuclease 1 ( FEN 1 ) , DNA polymerase delta , and DNA ligase 1 . ^^^ Disruption of the PCNA binding site of either FEN 1 or DNA ligase 1 significantly reduced efficiency of AP site repair but did not affect repair patch size . . ^^^ |
|
| Interacting proteins: P18858 and P12004 |
Pubmed |
SVM Score :0.0 |
| Complete repair was achieved by including highly purified human DNA polymerase delta or epsilon , PCNA , RFC , and DNA ligase 1 in reaction mixtures , reconstituting adduct repair for the first time with recombinant incision factors and human replication proteins . . ^^^ |
|
| Interacting proteins: P18858 and P12004 |
Pubmed |
SVM Score :0.0 |
| Using a human lymphoblastoid cell line , we have analysed , following gamma irradiation ( 0 . 5 , 1 , 2 , 4 , 8 , 16 and 32 Gy , at 0 . 5 , 24 , 48 and 72 h after treatment ) , the correlation between proliferation , cell cycle analysis , apoptosis and micronuclei frequency with the expression of TP 53 , WAF 1 , DNA LIGASE 1 , PCNA , BAX , BLC 2 , BAK , DAD 1 , ICH 1 Long and Short forms mRNAs . ^^^ We have found that whereas TP 53 , BAK , ICH 1 Short form , and DAD 1 were expressed at constant levels , WAF 1 , PCNA , BAX were up regulated , ICH 1 Long form , DNA LIGASE 1 , and BCL 2 were down regulated . ^^^ |
|
| Interacting proteins: P18858 and P12004 |
Pubmed |
SVM Score :0.0 |
| This ternary PCNA Cdk 2 cyclin A complex was able to phosphorylate the PCNA binding region of the large subunit of replication factor C as well as DNA ligase 1 . ^^^ Furthermore , PCNA appears to be a connector between Cdk 2 and DNA ligase 1 and to stimulate phosphorylation of DNA ligase 1 . ^^^ |
|
| Interacting proteins: P18858 and P12004 |
Pubmed |
SVM Score :0.0 |
| Here we show that etoposide , an anticancer drug that induces double strand breaks , triggers the redistribution of DNA ligase 1 and proliferating cell nuclear antigen from replicative patterns and the ensuing dephosphorylation of DNA ligase 1 . ^^^ |
|
| Interacting proteins: P18858 and P12004 |
Pubmed |
SVM Score :0.0 |
| We show here that p 21 also inhibits PCNA stimulation of long patch base excision repair ( BER ) in vitro . p 21 disrupts PCNA directed stimulation of flap endonuclease 1 ( FEN 1 ) , DNA ligase 1 , and DNA polymerase delta . ^^^ |
|
| Interacting proteins: P18858 and P12004 |
Pubmed |
SVM Score :0.0 |
| The cellular proteins flap endonuclease 1 ( FEN 1 ) , proliferating cell nuclear antigen , replication factor C , DNA ligase 1 and DNA polymerase delta are required for the repair of this type of DNA lesion . ^^^ |
|
| Interacting proteins: P18858 and P12004 |
Pubmed |
SVM Score :0.0 |
| The PCNA heterotrimer , but not individual subunits , stimulates the activities of the DNA polymerase , DNA ligase 1 , and the flap endonuclease ( FEN 1 ) of S . solfataricus . ^^^ |
|
| Interacting proteins: P18858 and P12004 |
Pubmed |
SVM Score :0.0 |
| Cell cycle dependent phosphorylation of human DNA ligase 1 at the cyclin dependent kinase sites . ^^^ |
|
| Interacting proteins: P18858 and P12004 |
Pubmed |
SVM Score :0.0 |
| DNA synthesis by DNA polymerase delta / epsilon is dependent on proliferating cell nuclear antigen , which also stimulates the DNA ligase 1 and flap endonuclease . ^^^ |
|
| Interacting proteins: P18858 and P12004 |
Pubmed |
SVM Score :0.0 |
| We have used DNase 1 to release DNA bound PCNA together with replication proteins including the p 125 catalytic subunit of DNA polymerase delta ( p 125 pol delta ) , DNA ligase 1 , cyclin A , and cyclin dependent kinase 2 ( CDK 2 ) . ^^^ Interaction with these proteins was investigated by immunoprecipitation with antibodies binding near the interdomain connector loop or to the C terminal domain of PCNA , respectively , or with antibodies to p 125 pol delta or DNA ligase 1 . ^^^ PCNA interaction with p 125 pol delta or DNA ligase 1 was detected only by the latter antibodies , and found to be mutually exclusive . ^^^ A GST p 21 ( waf1 / cip1 ) C terminal peptide displaced p 125 pol delta and DNA ligase 1 , but not CDK 2 , from PCNA . ^^^ Among them , p 125 pol delta and DNA ligase 1 interact with PCNA in a mutually exclusive manner . . ^^^ |
|
| Interacting proteins: P18858 and P12004 |
Pubmed |
SVM Score :0.0 |
| The recruitment of DNA ligase 1 to replication foci and the efficient joining of Okazaki fragments is dependent on the interaction between DNA ligase 1 and proliferating cell nuclear antigen ( PCNA ) . ^^^ Although the PCNA sliding clamp tethers DNA ligase 1 to nicked duplex DNA circles , the interaction does not enhance DNA joining . ^^^ Although RFC inhibited DNA joining by DNA ligase 1 , the addition of PCNA alleviated inhibition by RFC . ^^^ Notably , the effect of PCNA on ligation was dependent on the PCNA binding site of DNA ligase 1 . ^^^ |
|
| Interacting proteins: P18858 and P12004 |
Pubmed |
SVM Score :0.0 |
| Acute Rb excision from conditional knockout myotubes caused reexpression of E2F transcriptional activity , cyclin E and A kinase activities , PCNA , DNA ligase 1 , RPA , and MCM 2 , but did not induce DNA synthesis , showing that pRb is not indispensable to preserve the postmitotic state of these cells . ^^^ |
|
| Interacting proteins: P18858 and P12004 |
Pubmed |
SVM Score :0.0 |
| Similarities in the toroidal shape and dimensions of DNA ligase 1 and the proliferating cell nuclear antigen sliding clamp are suggestive of an extensive protein protein interface that may coordinate the joining of Okazaki fragments . . ^^^ |
|
| Interacting proteins: P18858 and P12004 |
Pubmed |
SVM Score :0.0 |
| Originally detected in fixed cells , DNA replication foci ( RFi ) were later visualized in living cells by using green fluorescent protein ( GFP ) tagged proliferating cell nuclear antigen ( PCNA ) and DNA ligase 1 . ^^^ |
|
| Interacting proteins: P18858 and P12004 |
Pubmed |
SVM Score :0.0 |
| Notably , the ability of DNA ligase 1 to promote the recombinational repair of DNA double strand breaks was dependent upon its interaction with proliferating cell nuclear antigen . ^^^ |
|
| Interacting proteins: P18858 and P12004 |
Pubmed |
SVM Score :0.0 |
| Together our genetic and biochemical analysis suggests that , although DNA ligase 1 seals DNA nicks during replication , repair , and recombination , higher than normal levels can yield genetic instability by disrupting the normal interplay of PCNA with other proteins such as Fen1 . . ^^^ |
|
| Interacting proteins: P18858 and P12004 |
Pubmed |
SVM Score :0.0 |
| We have used a combination of in situ extraction and dual color photobleaching to compare the dynamic properties of three proteins essential for lagging strand synthesis : the polymerase clamp proliferating cell nuclear antigen ( PCNA ) and two proteins that bind to it , DNA Ligase 1 and Fen 1 . ^^^ |
|
| Interacting proteins: P18858 and P12004 |
Pubmed |
SVM Score :0.0 |
| The proteins and enzymatic activities that were found to copurify with the NB DNA synthesome include : DNA polymerases alpha , delta , and epsilon , proliferating cell nuclear antigen , replication factor A , replication factor C , topoisomerases 1 and 2 , flap endonuclease 1 , and DNA ligase 1 . ^^^ |
|
| Interacting proteins: P18858 and P12004 |
Pubmed |
SVM Score :0.0 |
| These results suggest that PCNA mediates the entry of the flap endonuclease and DNA ligase 1 into the process of Okazaki fragment joining , and this ordered entry is necessary to prevent CAG repeat tract expansions . . ^^^ Interactions among DNA ligase 1 , the flap endonuclease and proliferating cell nuclear antigen in the expansion and contraction of CAG repeat tracts in yeast . ^^^ |
|
| Interacting proteins: P18858 and P12004 |
Pubmed |
SVM Score :0.0 |
| These functions include loading onto DNA by replication factor C , as well as Okazaki fragment synthesis and maturation by the PCNA coordinated actions of DNA polymerase delta , the flap endonuclease FEN 1 , and DNA ligase 1 . ^^^ |
|
| Interacting proteins: P18858 and P12004 |
Pubmed |
SVM Score :0.0 |
| Etoposide also triggers the dispersal of replicative proteins , proliferating cell nuclear antigen and DNA ligase 1 , from replication factories . ^^^ |
|
| Interacting proteins: P18858 and P12004 |
Pubmed |
SVM Score :0.0 |
| It binds the DNA glycosylase MutY homolog , and stimulates DNA polymerase beta , flap endonuclease 1 , and DNA ligase 1 . 9 1 1 resembles proliferating cell nuclear antigen ( PCNA ) , which stimulates some of these same repair enzymes , and is loaded onto DNA in a similar manner . ^^^ Unlike PCNA , 9 1 1 stimulates DNA ligase 1 activity to the same extent on both linear and circular substrates , indicating that encirclement is not a requirement for stimulation . ^^^ |
|
| Interacting proteins: P18858 and P12004 |
Pubmed |
SVM Score :0.0 |
| During DNA replication , the joining of Okazaki fragments by the LIG 1 gene product appears to be mediated by an interaction with proliferating cell nuclear antigen ( PCNA ) . ^^^ |
|
| Interacting proteins: P18858 and P12004 |
Pubmed |
SVM Score :0.0 |
| In the nucleus , Lig 1 interacts with the sliding clamp proliferating cell nuclear antigen ( PCNA ) via a conserved PCNA interacting sequence motif known as a PIP box . ^^^ |
|
| Interacting proteins: P18858 and P12004 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P18858 and P12004 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P18858 and P12004 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P18858 and P12004 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P18858 and P12004 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P18858 and P12004 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P18858 and P12004 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P18858 and P12004 |
Pubmed |
SVM Score :0.0 |
| NA |
|