Pubmed abstracts for Protein-Protein Interaction search result :

Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.62983137
We show that KDR mRNA is co expressed with flt 1 in vascular cells in glioblastoma but not in low grade glioma . 0.62983137^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.50303774
No significant association was found between FLK 1 , FLT 1 , VEGF and eNOS immunoreactivity and vascular density . 0.50303774^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Receptor chimeras indicate that the vascular endothelial growth factor receptor 1 ( VEGFR 1 ) modulates mitogenic activity of VEGFR 2 in endothelial cells . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
MATERIALS AND METHODS : Tumors from 45 patients were assessed by immunohistochemistry for expression patterns of five growth factors and their receptors ; platelet derived growth factor A chain ( PDGF AA ) and PDGF receptor alpha ( PDGFalphaR ) , PDGF BB and PDGFbetaR , transforming growth factor alpha ( TGFalpha ) and its receptor epidermal growth factor receptor ( EGFR ) and vascular endothelial growth factor ( VEGF ) and its receptors vascular endothelial growth factor receptor 1 ( FLT 1 ) and vascular endothelial growth factor receptor 2 ( FLK 1 / KDR ) , two growth inhibiting factors ; transforming growth factor beta 1 ( TGFbeta 1 ) and TGFbeta 2 and their receptor couple TGFbeta receptor 1 ( TGFbetaR 1 ) and TGFbetaR 2 , and basic fibroblast growth factor ( bFGF ) . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
We investigated the expression pattern of NP 1 / NP 2 , their co receptors , vascular endothelial growth factor receptor 1 and 2 ( VEGFR 1 , VEGFR 2 ) , and their ligands , class 3 semaphorins , VEGF and PLGF , following experimental cerebral ischemia in mice . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Activation of vascular endothelial growth factor receptor 1 and 2 ( VEGFR 1 and VEGFR 2 ) plays a critical role in vasculogenesis and angiogenesis . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
High expression of vascular endothelial growth factor was strongly correlated with high expression of vascular endothelial growth factor receptor 2 ( but not vascular endothelial growth factor receptor 1 ) on the cancer cells ( P < 0 . 001 ) . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
The mRNA levels of 13 genes including CD 147 ( receptor for CypA ) , PDGF BB , endothelin 1 ( ET 1 ) , vascular endothelial growth factor receptor 1 ( VEGFR 1 ) , VEGFR 2 , VEGFR 3 , neuropilin 1 ( NRP 1 ) , NRP 2 , eNOS , iNOS , nNOS , ICAM 1 , and PECAM 1 were semiquantitatively determined by real time RT PCR as standardized with a house keeping gene beta actin . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Vascular endothelial growth factor receptor 1 ( VEGFR 1 ) and receptor 2 ( VEGFR 2 ) , are the high affinity VEGF receptors , which play an important role in de novo blood vessel formation and hematopoietic cell development . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Breast cancer metastases to bone were immunohistochemically stained for VEGF , its receptors VEGFR 1 and 2 ( vascular endothelial growth factor receptor 1 and 2 ) , demonstrating that breast cancer metastases express VEGF strongly and that surrounding osteoclasts express both VEGFR 1 and VEGFR 2 . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Role of vascular endothelial growth factor receptor 1 and vascular endothelial growth factor receptor 2 in the vasodilator response to vascular endothelial growth factor in the neonatal piglet lung . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Formalin fixed , paraffin embedded sections of laryngeal squamous papillomas from the 12 patients with a diagnosis of RRP and the 5 control patients were examined by in situ hybridization for the presence of messenger RNA ( mRNA ) for VEGF A and vascular endothelial growth factor receptor 1 ( VEGFR 1 ) and vascular endothelial growth factor receptor 2 ( VEGFR 2 ) . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Anti ATF 3 small interfering RNA gave an inhibitory influence on tube formation by NP 31 cells expressing an activated form of the vascular endothelial growth factor receptor 1 ( VEGFR 1 ) kinase . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
To analyze the molecular mechanisms underlying glioma angiogenesis , we studied the expression of vascular endothelial growth factor ( VEGF ) and its tyrosine kinase receptors VEGFR 1 and VEGFR 2 during normal brain development and glioma induced angiogenesis . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Angiogenesis , the sprouting of new blood vessels from pre existing ones , and the permeability of blood vessels are regulated by vascular endothelial growth factor ( VEGF ) via its two known receptors Flt 1 ( VEGFR 1 ) and KDR / Flk 1 ( VEGFR 2 ) . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
We have cloned partial cDNAs encoding bovine VEGFR 1 ( flt ) and 2 ( flk 1 ) and used them to study VEGFR expression by bovine microvascular and large vessel derived endothelial cells . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
The endothelial cell mitogen vascular endothelial growth factor ( VEGF ) stimulates angiogenesis , and together with its two receptor tyrosine kinases VEGFR 1 ( FLT 1 ) and VEGFR 2 ( KDR ) , is up regulated during the malignant progression of gliomas . ^^^ Significantly elevated levels of Tie mRNA , Tie protein , VEGFR 1 , and VEGFR 2 but not FLT 4 mRNAs were observed in the endothelia of both tumor types . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Quek 1 and 2 display a lower homology to fit 1 ( VEGFR 1 ) ( about 45 % aa identity ) . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
However , the finding of dramatic up regulation of vascular endothelial growth factor ( VEGF ) , a potent endothelial cell growth factor with vascular permeability inducing activity , in stromal cells and the corresponding receptors , VEGFR 1 and VEGFR 2 , in tumor endothelial cells suggests that angiogenesis and cyst formation in hemangioblastomas may be regulated by this signaling pathway via a paracrine mechanism . . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Vascular endothelial growth factor ( VEGF ) and its receptors , Flt 1 ( VEGFR 1 ) and Flk 1 ( VEGFR 2 ) , as well as Angiopoietin 1 and its receptor , Tie 2 , represent key signal transduction systems involved in the regulation of embryonic vascular development . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Vascular endothelial growth factor ( VEGF ) and its tyrosine kinase receptors VEGFR 1 ( flt 1 ) and VEGFR 2 ( flk 1 / KDR ) are key mediators of physiological and pathological angiogenesis . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
We found that the mRNA for the VEGF and VEGF C binding VEGFR 2 ( KDR / Flk 1 ) was stimulated by IL 1beta in human umbilical vein endothelial cells , whereas the mRNA levels of VEGFR 1 ( Flt 1 ) and VEGFR 3 ( Flt 4 ) were not altered . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
These mice demonstrated an increased density of tortuous cutaneous blood capillaries with elevated expression levels of the high affinity VEGF receptors , VEGFR 1 and VEGFR 2 , most prominently during the neonatal period . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
In the present study , the localization and magnitude of VEGF , VEGF receptor 1 ( VEGFR 1 ) , and VEGF receptor 2 ( VEGFR 2 ) gene expression were examined in the eye of streptozotocin induced diabetic rats using quantitative in situ hybridization . ^^^ The distributions of VEGFR 1 and VEGFR 2 were similar in both control and diabetic rats . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Characterization of VEGF receptor ( VEGFR ) expression revealed that BME , BAE , and BLE cell lines express VEGFR 1 and 2 , whereas of the three cell lines assessed , only BAE cells express VEGFR 3 . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Furthermore , VEGF E bound KDR / Flk 1 ( VEGFR 2 ) and induced its autophosphorylation to almost the same extent as VEGF 165 , but did not bind Flt 1 ( VEGFR 1 ) nor induce autophosphorylation of Flt 1 . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Accordingly , we studied the regulation of Flt 1 ( VEGFR 1 ) and KDR / Flk 1 ( VEGFR 2 ) expression in a mouse model of ischemia induced retinal neovascularization . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Intriguingly , the expression of VEGF receptor 1 ( VEGFR 1 ) was detected in certain spermatogenic cells in addition to vascular endothelium , and both VEGFR 1 and VEGFR 2 were also found in the Leydig cells of the testis . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Furthermore , in addition to VEGF itself , expression of VEGF receptor 1 ( VEGFR 1 ) , but not VEGFR 2 , was induced by hypoxia in endothelial cells of lung , heart , brain , kidney , and liver . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
The various VEGF forms bind to two tyrosine kinase receptors , VEGFR 1 ( flt 1 ) and VEGFR 2 ( KDR / flk 1 ) , which are expressed almost exclusively in endothelial cells . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
A novel vascular endothelial growth factor encoded by Orf virus , VEGF E , mediates angiogenesis via signalling through VEGFR 2 ( KDR ) but not VEGFR 1 ( Flt 1 ) receptor tyrosine kinases . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
VEGF binds and activates two tyrosine kinase receptors called VEGFR 1 and VEGFR 2 and neuropilin 1 . ^^^ The elucidation of the transduction pathways of each receptor suggests that VEGFR 1 mediates cell migration whereas VEGFR 2 mediates cell proliferation . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Vascular endothelial growth factor ( VEGF ) and its specific receptors Flt 1 ( VEGFR 1 ) and KDR ( VEGFR 2 ) compose potent ligand receptor systems involved in angiogenesis and microvascular hyperpermeability . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
In the first trimester trophoblast cells , PIGF 1 increased the association of phosphorylated extracellular signal related kinase ( ERK ) with VEGFR 1 immunoprecipitates while both PIGF 1 and PIGF 2 also potentiated endogenous VEGF mediated association of phosphorylated extracellular related kinase ( ERK ) with VEGFR 2 ( KDR ) . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
These molecules mediate endothelial cell proliferation , vascular permeability , angiogenesis , and lymphangiogenesis via the endothelial cell receptors VEGFR 1 ( Flt 1 ) , VEGFR 2 ( KDR / Flk1 ) , and VEGFR 3 ( Flt 4 ) . ^^^ ORFV 2 VEGF was found to bind and induce autophosphorylation of VEGFR 2 and was unable to bind or activate VEGFR 1 and VEGFR 3 , but bound the newly identified VEGF 165 receptor neuropilin 1 . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
VEGF , VEGFR 1 , VEGFR 2 , microvessel density and endothelial cell proliferation in tumours of the ovary . ^^^ VEGF , VEGFR 1 , VEGFR 2 and EC proliferation were examined immuno histochemically , and in situ hybridisation ( ISH ) studies of VEGF mRNA expression were performed and assessed in regions of high ( HVD ) and average ( AVD ) vessel density . ^^^ VEGF immunostaining did not differ between tumour types ; however , the percentage of VEGFR 1 and VEGFR 2 positive vessels was significantly lower in mucinous tumours . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
We used the middle cerebral artery occlusion model ( MCAO ) in the rat to investigate VEGF mRNA and protein localization , and VEGFR 1 mRNA and VEGFR 2 mRNA expression in cerebral ischemia . ^^^ By nonradioactive in situ hybridization we observed upregulation of VEGF mRNA and VEGFR 1 mRNA , but not of VEGFR 2 mRNA in the hemisphere ipsilateral to MCA occlusion . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Its biological activity is mediated by two receptor tyrosine kinases , VEGFR 1 ( Flt 1 ) and VEGFR 2 ( KDR ) . ^^^ In contrast to VEGFR 2 , a naturally occurring soluble form of the VEGFR 1 ( sVEGFR 1 ) is produced by endothelial cells by differential splicing of the flt 1 gene , and it is a secreted gene product . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
By immunohistochemistry in 30 human eyes and in two cynomolgus monkey eyes , KDR ( VEGFR 2 ) and flt 4 ( VEGFR 3 ) were preferentially localized at the side of the CC endothelium facing the RPE cell layer , whereas flt 1 ( VEGFR 1 ) was found on the inner CC and on other choroidal vessels . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Constitutive expression of VEGF , VEGFR 1 , and VEGFR 2 in normal eyes . ^^^ PURPOSE : The expression of vascular endothelial growth factor ( VEGF ) and its high affinity receptors VEGFR 1 and VEGFR 2 was investigated in normal eyes . ^^^ Ocular VEGF , VEGFR 1 , and VEGFR 2 expression was studied using a combination of in situ hybridization , northern blot analysis , immunohistochemistry , immunoassay , and reverse transcription polymerase chain reaction . ^^^ VEGFR 1 and VEGFR 2 mRNAs were detected in normal monkey iris , retina , and the choroid retinal pigment epithelial complex . ^^^ In both monkey and rat eyes , VEGFR 1 and VEGFR 2 mRNAs were localized to the inner nuclear layer of the retina . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
The following patterns were observed : The endocardium was positive for VEGFR 1 , VEGFR 2 , NP 1 , Tie 1 , and Tie 2 but negative for VEGFR 3 . ^^^ The coronary vessels were positive for Tie 1 , Tie 2 , VEGFR 1 , and NP 1 and negative for VEGFR 2 and VEGFR 3 . ^^^ Myocardial capillaries and epicardial blood vessels stained for VEGFR 1 , VEGFR 2 , NP 1 , and Tie 1 ; myocardial capillaries and epicardial veins weakly for Tie 2 ; and epicardial lymphatic vessels for VEGFR 2 and VEGFR 3 , weakly for Tie 1 and Tie 2 , but not for VEGFR 1 or NP 1 . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
In a PlGF positive tumour sample , immunoreactive VEGFR 1 and VEGFR 2 were detected in endothelial cells of the blood vessels . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
The VEGF receptors fms like tyrosine kinase 1 ( Flt 1 ) / VEGFR 1 and kinase insert domain containing receptor ( KDR ) / VEGFR 2 are up regulated on the surface of endothelial cells ( ECs ) in gliomas . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
These activities are at least in part mediated by binding to two high affinity receptors , VEGFR 1 and VEGFR 2 . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Vascular endothelial growth factor ( VEGF ) is one of the key factors in tumor neoangiogenesis , acting through its receptors KDR ( VEGFR 2 ) and fit 1 ( VEGFR 1 ) expressed on endothelial cells . ^^^ Our data demonstrate that VEGFR 1 and to a lesser extent VEGFR 2 are expressed in a number of human tumor tissues and derived cells in culture . ^^^ VEGFR 1 protein is expressed in 26 of 42 glioma tissues , 22 of which show a coexpression of VEGFR 1 with VEGFR 2 ; 1 glioma tissue expresses exclusively VEGFR 2 . ^^^ In the derived glioma cell cultures , we found VEGFR 1 mRNA expression in 6 of 11 cultures , with one coexpressing VEGFR 1 and VEGFR 2 . ^^^ In addition VEGFR 1 protein expression was demonstrated in 30 of 37 tumor tissues of squamous cell carcinomas of the head and neck , with VEGFR 2 coexpression in 15 tissues and an expression of VEGFR 2 alone in 1 tissue . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Compared with controls , the MA and BO groups showed : ( 1 ) diminished placental trophoblastic VEGF immunoreactivity ; ( 2 ) weaker VEGFR 1 and 2 immunoreactivity in decidual vascular endothelium ; ( 3 ) reduced placental trophoblastic Tie 1 receptor immunoreactivity ; and ( 4 ) reduced decidual vascular endothelial Tie 1 and 2 receptor immunoreactivity . ^^^ Interestingly , placental villi from the BO group presented blood vessel like structures negative for von Willebrand factor , but positive for VEGF , VEGFR 1 , 2 , 3 , Tie 1 and Tie 2 receptor . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
VEGF acts via two tyrosine kinase family receptors : VEGFR 1 ( Flt 1 ) and VEGFR 2 ( KDR / Flk 1 ) . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Vascular permeability factor / vascular endothelial growth factor ( VPF / VEGF ) interacts with two high affinity tyrosine kinase receptors , VEGFR 1 and VEGFR 2 , to increase microvascular permeability and induce angiogenesis . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Using porcine endothelial cells expressing one or the other type of the VEGF receptors , VEGFR 1 or VEGFR 2 , or human umbilical vein endothelial cells pretreated with a VEGFR 2 neutralizing antibody , we show that VEGFR 2 is responsible for VEGF induced activation of the stress activated protein kinase 2 / p38 ( SAPK2 / p38 ) , phosphorylation of focal adhesion kinase ( FAK ) , and enhanced migratory activity . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Overexpression of VEGF 121 in immortalized endothelial cells causes conversion to slowly growing angiosarcoma and high level expression of the VEGF receptors VEGFR 1 and VEGFR 2 in vivo . ^^^ The angiosarcomas generated from MS 1 VEGF cells demonstrated up regulation of the VEGF receptors VEGFR 2 and VEGFR 1 ( Flt 1 ) in vivo compared with benign hemangiomas generated from MS 1 cells . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
It mediates its activity mainly via two tyrosine kinase receptors , VEGFR 1 ( flt 1 ) and VEGFR 2 ( flk 1 / KDR ) , although other receptors , such as neuropilin 1 and 2 , can also bind VEGF . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Release and complex formation of soluble VEGFR 1 from endothelial cells and biological fluids . ^^^ One of the key molecules promoting angiogenesis is the endothelial cell specific mitogen , vascular endothelial growth factor ( VEGF or VEGF A ) , which acts through two high affinity receptor tyrosine kinases ( VEGFR ) , VEGFR 1 ( or Flt 1 ) and VEGFR 2 ( or KDR / Flk 1 ) . ^^^ It was shown before that a soluble variant of VEGFR 1 ( sVEGFR 1 ) can be generated by differential splicing of the flt 1 mRNA . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
In addition , expression of the VEGF receptors , VEGFR 1 , VEGFR 2 , and VEGF165R / neuropilin , coincided with expression of their ligands , being maximally expressed during mineralization . ^^^ Although treatment of KS 483 cells with soluble FLT 1 , an agent that blocks binding of VEGF A and B to VEGFR 1 , did not inhibit nodule formation , this observation does not exclude involvement of VEGFR 2 in the regulation of osteoblast differentiation . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Here we report pharmacodynamic studies testing anti Flt 1 ( VEGFR 1 ) and anti KDR ( VEGFR 2 ) ribozymes in animal models of solid tumor growth and metastasis . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
The stimulatory effect of CNF 1 was specific for VEGFR 2 since the phosphotyrosine content of VEGFR 1 was not affected by the toxin . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Vascular endothelial growth factor ( VEGF ) acts primarily as an endothelial cell mitogen via the `` endothelial cell specific ' ' receptors VEGFR 1 ( flt 1 ) and VEGFR 2 ( flk 1 / KDR ) . ^^^ NRK 52 E expressed VEGFR 1 and VEGFR 2 mRNA and protein by RT PCR , Northern blotting , Western blotting , immunofluorescence , and ligand binding . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
VEGF receptor ( VEGFR 1 and 2 ) expression was determined using reverse transcription polymerase chain reaction and fluorescence activated cell sorting , and affinity was measured using Scatchard analysis . ^^^ RPE cells expressed both VEGFR 1 and 2 in vitro ; however , the expression of VEGFR 1 was very low . ^^^ Pretreatment with TGF beta 2 ( 10 ng / ml ) was associated with increased expression of the VEGFR 1 in RPE cells and increased receptor affinity for VEGF detected by Scatchard analysis . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Vascular endothelial growth factor ( VEGF ) has two highly homologous tyrosine kinase receptors : Flt 1 ( VEGFR 1 ) and KDR ( VEGFR 2 ) . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
To determine whether angiogenesis and growth factor receptor expression are consistent findings in malignant melanoma , primary human melanomas were examined for mRNA expression of receptors for fibroblast growth factors ( FGFR 1 , FGFR 2 ) , vascular endothelial growth factor ( VEGFR 1 , VEGFR 2 ) , and the receptors Tiel and Tie 2 . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Vascular endothelial growth factor ( VEGF ) intracellular signaling in endothelial cells is initiated by the activation of distinct tyrosine kinase receptors , VEGFR 1 ( Flt 1 ) and VEGFR 2 ( Flk 1 / KDR ) . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
To investigate the mechanism of neuroprotection by VEGF , the expression of known target receptors for VEGF was measured by Western blotting , which showed that HN 33 cells expressed VEGFR 2 receptors and neuropilin 1 , but not VEGFR 1 receptors . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
VEGF receptor 2 [ VEGFR 2 ( KDR / Flk 1 ) ] mediates endothelial cell mitogenesis and permeability increases , whereas the role of VEGF receptor 1 [ VEGFR 1 ( Flt 1 ) ] has not been clearly defined . ^^^ In the present study , a monoclonal antibody , 2C3 , is shown to block the interaction of VEGF with VEGFR 2 but not with VEGFR 1 through ELISA , receptor binding assays , and receptor activation assays . 2C3 blocks the VEGF induced vascular permeability increase in guinea pig skin . 2C3 has potent antitumor activity , inhibiting the growth of newly injected and established human tumor xenografts in mice . ^^^ These findings demonstrate the usefulness of 2C3 in dissecting the pathways that are activated by VEGF in cells that express both VEGFR 1 and VEGFR 2 , as well as highlighting the dominant role of VEGFR 2 in mediating VEGF induced vascular permeability increase and tumor angiogenesis . . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
A possible contribution of VEGF A to the increased formation under hypoxic conditions is unlikely because there was no increased VEGF A expression detected under hypoxic conditions , and the hypoxia induced tube formation by FGF 2 and TNF alpha was not inhibited by soluble VEGFR 1 ( sVEGFR 1 ) , or by antibodies blocking VEGFR 2 . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Vascular endothelial growth factor ( VEGF ) , through activation of its endothelial receptors VEGFR 1 and VEGFR 2 , is an important positive modulator of tumor angiogenesis and edema in solid tumors such as malignant astrocytomas . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
The angiogenesis / vasculogenesis pathway is required for embryonic survival and includes several receptors ( VEGFR 1 , VEGFR 2 , Tie 2 ) and ligands ( VEGF , Ang 1 , Ang 2 , neuropillin ) . ^^^ VEGFR 1 may be primarily responsible for binding of VEGF to yolk sac and embryonic tissues , as masking VEGFR 2 did not reduce VEGF binding in those areas , and it is interesting that major structural defects were also not found in those regions . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
In contrast to previous reports which show a limited ability of VEGFR 1 to mediate signalling cascades , we show that once sufficiently activated , VEGFR 1 signals in a similar manner to VEGFR 2 in endothelial cells . . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
VEGFR 1 ( Flt 1 ) , VEGFR 2 ( KDR ) and VEGFR 3 ( Flt 4 ) are endothelial specific receptor tyrosine kinases , regulated by members of the vascular endothelial growth factor family . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Vascular endothelial growth factor ( VEGF ) and its receptors VEGFR 1 and VEGFR 2 , fibroblast growth factor 2 ( FGF 2 ) and its receptors FGFR 1 and FGFR 2 , as well as epidermal growth factor ( EGF ) and its receptor EGFR are believed to be important in the control of angiogenesis in the human endometrium . ^^^ The receptors VEGFR 1 , VEGFR 2 , FGFR 2 and EGFR were co expressed and exhibited their strongest expression during the beginning of the secretory phase , coinciding with the developing endometrial oedema and formation of a complex subepithelial capillary plexus . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Here we show that VEGF receptor 1 ( VEGFR 1 ) negatively regulates VEGFR 2 mediated proliferation via nitric oxide ( NO ) in an epithelial cancer cell line ECV 304 . ^^^ Cell cycle analysis showed that inhibition of VEGFR 1 increased the transition from G ( 1 ) to S phase whereas inhibition of VEGFR 2 blocked the VEGF mediated transition from G ( 1 ) to S phase . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
While VEGF binds to two receptor protein tyrosine kinases , VEGFR 1 ( Flt 1 ) and VEGFR 2 ( KDR ) , most biological functions of VEGF are mediated via VEGFR 2 , and the role of VEGFR 1 is currently unknown . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Measurement of VEGF B together with other angiogenic factors may identify a poor prognostic patient group , which may benefit from anti VEGF receptor therapy targeted to flt 1 ( VEGFR 1 ) as well as kdr ( VEGFR 2 ) . . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
SU 5416 , a potent inhibitor of the VEGF receptor 1 ( VEGFR 1 ) and VEGFR 2 receptor tyrosine kinases , did not abolish the inhibitory effect of VEGF , indicating that the VEGF effect is mediated by a mechanism unrelated to VEGFR 1 or VEGFR 2 tyrosine kinase activity . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
To elucidate the sequence of molecular events intricate with angiogenesis and the initiation and progression prostate cancer , the temporal and spatial expression patterns of platelet endothelial cell adhesion molecule 1 ( PECAM1 / CD31 ) , hypoxia induced factor 1alpha ( HIF 1alpha ) , vascular endothelial growth factor ( VEGF ) , and the cognate receptors VEGFR 1 and VEGFR 2 were characterized . ^^^ Immunoblot and in situ analyses further demonstrated a distinct late angiogenic switch consistent with decreased expression of VEGFR 1 , increased expression of VEGFR 2 , and the transition from a differentiated adenocarcinoma to a more poorly differentiated state . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Recently VEGF B and VEGF C , two new VEGF family members , have been identified that bind to the tyrosine kinase receptors flt 1 ( VEGFR 1 ) , KDR ( VEGFR 2 ) , and flt 4 ( VEGFR 3 ) . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Vascular permeability factor / vascular endothelial growth factor ( VPF / VEGF ) exerts its multiple functions by activating two receptor tyrosine kinases , Flt 1 ( VEGFR 1 ) and KDR ( VEGFR 2 ) , both of which are selectively expressed on primary vascular endothelium . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Additionally , we summarize the properties of 2C3 , a novel monoclonal anti VEGF antibody that blocks VEGF from binding to VEGFR 2 but not VEGFR 1 . 2C3 may be utilized as both an anti angiogenic agent by inhibiting VEGFR 2 activity and potentially as a vascular targeting agent by binding to blood vessels that express the VEGF VEGFR 1 complex . . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Vascular endothelial growth factor ( VEGF ) is known to be a highly specific mitogen for endothelial cells through two high affinity tyrosine kinase receptors , VEGFR 1 and VEGFR 2 , which are almost specifically expressed in endothelial cells . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Here we report that VEGF stimulated nitric oxide ( NO ) release is inhibited by blockade of VEGFR 1 and that VEGFR 1 via NO negatively regulates of VEGFR 2 mediated proliferation and promotes formation of capillary networks in human umbilical vein endothelial cells ( HUVECs ) . ^^^ Porcine aortic endothelial ( PAE ) cells expressing human VEGFR 1 but not VEGFR 2 plated on growth factor reduced Matrigel rearranged into tube like structures that were prevented by anti VEGFR 1 antibody or a cGMP inhibitor . ^^^ VEGF stimulated NO release from VEGFR 1 but not VEGFR 2 transfected endothelial cells and placenta growth factor 1 stimulated NO release in HUVECs . ^^^ These data indicate that VEGFR 1 is a signaling receptor that promotes endothelial cell differentiation into vascular tubes , in part by limiting VEGFR 2 mediated endothelial cell proliferation via NO , which seems to be a molecular switch for endothelial cell differentiation . . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
We recently have shown that certain leukemias not only produce VEGF but also selectively express functional VEGF receptors ( VEGFRs ) , such as VEGFR 2 ( Flk 1 , KDR ) and VEGFR 1 ( Flt 1 ) , resulting in the generation of an autocrine loop . ^^^ Long term remission with no evidence of disease was achieved only if mice were treated with mAbs against both murine and human VEGFR 2 , whereas mAbs against human or murine VEGFR 1 had no effect on mice survival . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Although targeting of either VEGFR 1 or VEGFR 2 alone partially blocks the growth of tumors , inhibition of both VEGFR 1 and VEGFR 2 was necessary to completely ablate tumor growth . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
We cultured human microvascular endothelial cells on different matrices ( vitronectin , laminin , fibronectin , fibrin , and collagen 4 ) and examined for the presence of growth factor receptors ( FGFR 1 , FGFR 2 , VEGFR 1 , and VEGFR 2 ) . ^^^ We show that vitronectin increased the presence of all four growth factor receptors and most notably , VEGFR 1 . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Vascular permeability factor / vascular endothelial growth factor ( VPF / VEGF ) promotes its function primarily by activating two receptor tyrosine kinases , Flt 1 ( VEGFR 1 ) and KDR ( VEGFR 2 ) . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
High expression levels of flk 1 ( VEGFR 2 ) , flt 1 ( VEGFR 1 ) , tie 1 , and tie 2 are also found , indicating that budding endothelial cells displayed an angiogenic phenotype . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
VEGF initiates signaling by dimerizing the receptors VEGFR 1 and VEGFR 2 . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
VEGF exerts its biologic activities through 2 transmembrane tyrosine kinase receptors : the fms like tyrosine kinase receptor ( Flt 1 , or VEGFR 1 ) and kinase insert domain containing receptor ( KDR or VEGFR 2 ) . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
SU 5416 is a competitive inhibitor of VEGF receptor subtypes VEGFR 1 and VEGFR 2 and stem cell factor receptor c kit . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Although much is known about the biology of VEGF and its cognate receptors , VEGFR 1 and VEGFR 2 , the role of a recently identified co receptor for VEGF , neuropilin 1 , is not well understood . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
VEGF A is a ligand for the two receptor tyrosine kinases VEGFR 1 ( Flt 1 ) and VEGFR 2 ( KDR / Flk 1 ) . ^^^ Most biological functions of VEGF A are mediated via VEGFR 2 , whereas the role of VEGFR 1 is largely unknown . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Vascular endothelial growth factor ( VEGF ) mRNA becomes markedly up regulated by Nox 1 both in cultured cells and in tumors , and VEGF receptors ( VEGFR 1 and VEGFR 2 ) are highly induced in vascular cells in Nox 1 expressing tumors . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
The biological effects of VEGF are mediated by two tyrosine kinase receptors , Flt 1 ( VEGFr 1 ) and KDR ( VEGFR 2 ) . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
A probe against VEGFR 1 , a marker for endothelial cells only , revealed the same staining pattern . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
TSA inhibited in a dose dependent and reversible fashion the VEGF induced expression of VEGF receptors , VEGFR 1 , VEGFR 2 , and neuropilin 1 . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Involvement of VEGFR 2 ( kdr / flk 1 ) but not VEGFR 1 ( flt 1 ) in VEGF A and VEGF C induced tube formation by human microvascular endothelial cells in fibrin matrices in vitro . ^^^ Different forms of vascular endothelial growth factor ( VEGF ) and their cellular receptors ( VEGFR ) are associated with angiogenesis , as demonstrated by the lethality of VEGF A , VEGFR 1 or VEGFR 2 knockout mice . ^^^ Here we have used an in vitro angiogenesis model , consisting of human microvascular endothelial cells ( hMVEC ) cultured on three dimensional ( 3D ) fibrin matrices to investigate the roles of VEGFR 1 and VEGFR 2 in the process of VEGF A and VEGF C induced tube formation . ^^^ Soluble VEGFR 1 completely inhibited the tube formation induced by the combination of VEGF A and TNF alpha ( VEGF A / TNF alpha ) . ^^^ Blocking monoclonal antibodies specific for VEGFR 2 , but not antibodies specifically blocking VEGFR 1 , were able to inhibit the VEGF A / TNF alpha induced as well as the VEGF C / TNF alpha induced tube formation in vitro . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
In this study , we investigated the expression of VEGF related factor genes ( VEGF , VEGF B , and VEGF C ) and their receptor genes ( VEGFR 1 and VEGFR 2 ) in renal cell carcinoma ( RCC ) . ^^^ There were significant differences in the expression level of VEGF , VEGFR 1 and VEGFR 2 between RCC and the corresponding normal renal tissue . ^^^ The expression level of VEGF in the tumor tissue significantly correlated with those of VEGFR 1 and VEGFR 2 . ^^^ A moderate to high protein expression for VEGF , VEGFR 1 , and VEGFR 2 was observed in both the tumor cells and the endothelial cells , whereas the protein expression was low for VEGF B and VEGF C . ^^^ The present results suggested that VEGF and its receptors VEGFR 1 and VEGFR 2 cooperates to play a crucial role in the angiogenesis of RCC , while VEGF B and VEGFR C may not . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
METHODS : In retinas of 27 eyes of diabetic donors , 18 eyes of nondiabetic control donors , and 4 monkey eyes injected with PBS or VEGF A , expression patterns of VEGFR 1 , 2 , and 3 in relation to leaky microvessels , as identified by the marker pathologische anatomie Leiden endothelium ( PAL E ) were studied by immunohistochemistry . ^^^ In control human retinas and retinas of PBS injected monkey eyes , all three VEGFRs were expressed in nonvascular areas , but only VEGFR 1 was constitutively expressed in retinal microvessels . ^^^ In VEGF A injected monkey eyes , VEGFR 1 , 2 , and 3 and PAL E were expressed in retinal microvessels . ^^^ CONCLUSIONS : The VEGFR 1 , 2 , and 3 expression patterns in control retinas suggest physiological functions of VEGFs that do not involve the vasculature . ^^^ Initial vascular VEGF signaling may act primarily through VEGFR 1 . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Reverse transcription polymerase chain reaction ( RT PCR ) analysis revealed the expression of VEGF receptors VEGFR 1 ( Flt 1 ) , VEGFR 2 ( Kdr ) , and VEGFR 3 ( Flt 4 ) , as well as neuropilin 1 and 2 . ^^^ An in vitro kinase assay failed to demonstrate activation of VEGFR 2 upon stimulation with either VEGF E or VEGF A , consistent with the idea that the effect of VEGF A on primary human osteoblasts is mediated via VEGFR 1 . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
The receptors , VEGFR 1 ( FLT 1 ) and VEGFR 2 ( KDR / Flk 1 ) , are upregulated on vascular endothelial cells in tumors by hypoxia and by the increased local concentration of VEGF . ^^^ A promising monoclonal antibody is 2C3 , which blocks VEGF from binding to VEGFR 2 but not VEGFR 1 . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Invasive cytotrophoblasts in early gestation expressed VEGF A , VEGF C , placental growth factor ( PlGF ) , VEGFR 1 , and VEGFR 3 and , at term , VEGF A , PlGF , and VEGFR 1 . ^^^ In severe preeclampsia and hemolysis , elevated liver enzymes , and low platelets syndrome , immunolocalization on tissue sections showed that cytotrophoblast VEGF A and VEGFR 1 staining decreased ; staining for PlGF was unaffected . ^^^ Cytotrophoblast secretion of the soluble form of VEGFR 1 in vitro also increased . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Both VEGF and PlGF , acting through the tyrosine kinase receptors VEGFR 1 and VEGFR 2 , have been implicated in playing a role in ovine placental vascular development . ^^^ Both TN cotyledonary VEGFR 1 and VEGFR 2 mRNA expression levels rose significantly over the period studied ( P < 0 . 05 and P < 0 . 01 respectively ) . ^^^ Receptor mRNA concentration in HT cotyledonary tissue was significantly reduced at 90 dpc ( VEGFR 1 ; TN 0 . 21+ / 0 . 02 vs HT 0 . 11+ / 0 . 01 VEGFR 1 / actin , P < 0 . 05 , VEGFR 2 ; TN 0 . 18+ / 0 . 05 vs HT 0 . 07+ / 0 . 01 VEGFR 2 / actin , P < 0 . 01 ) . ^^^ Furthermore , alterations in VEGF , VEGFR 1 and VEGFR 2 mRNA expression , during the period of maximal placental growth , may contribute to the development of placental insufficiency , and ultimately intrauterine growth restriction . . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
The levels of vascular endothelial growth factor ( VEGF ) , its receptors VEGFR 1 and VEGFR 2 , and angiopoietin 1 mRNA tended to be higher in the mammary fat pad tumors than in the cranial tumors ( 1 . 5 , 1 . 5 , 3 , and 2 fold , respectively ) . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Renal ischemia reperfusion increases endothelial VEGFR 2 without increasing VEGF or VEGFR 1 expression . ^^^ Expression of the angiogenic growth factor vascular endothelial growth factor ( VEGF ) and its receptors ( VEGFR 1 and VEGFR 2 ) is up regulated by hypoxia in a variety of organs and cell lines . ^^^ RESULTS : VEGFR 2 mRNA and protein expression were up regulated without an increase in VEGF or VEGFR 1 expression . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Vascular Endothelial Growth Factor ( VEGF ) through interaction with its receptors VEGFR 2 and VEGFR 1 expressed on endothelial and hematopoietic stem cells promote recruitment of these cells into the sites of tissue injury accelerating vascular healing . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
As expected , puromycin resistant cells were positive for EGFP and also expressed several endothelial markers , including CD 31 , CD 34 , VEGFR 1 , VEGFR 2 , Tie 1 , VE cadherin and ICAM 2 . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
In pathological conditions , increased levels of expression of the VEGF receptors VEGFR 1 , VEGFR 2 , and VEGFR 3 accompany VEGF activity . ^^^ Furthermore , VEGFR 1 , but not VEGFR 2 and 3 , was present in microvessels in brain and retina . ^^^ Electron microscopy showed that VEGFR 1 expression was restricted to pericytes and VEGFR 2 to endothelial cells in normal vasculature of tonsils . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Among the known stem cell active chemokines , the angiogenic factor VEGF through interaction with its receptors , VEGFR 2 and VEGFR 1 expressed on endothelial and hematopoietic stem cells , promotes mobilization and recruitment of these cells into the neo angiogenic sites , thereby accelerating the revascularization process . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Here we demonstrate that VEGF and its receptors VEGFR 1 and VEGFR 2 are expressed in human surgical samples of HD . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
However , blocking VEGF by administering a soluble VEGFR 1 , which acts extracellularly , induced only minor effects . ^^^ Not only ligands selective for VEGF and VEGFR 2 but also VEGFR 1 agonists rescued survival and repopulation of VEGF deficient HSCs , revealing a function for VEGFR 1 signalling during haematopoiesis . . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
As VEGF exerts its effects via two receptors , VEGF receptor 1 ( VEGFR 1 ) and VEGFR 2 , we evaluated the significance of VEGFR 1 and VEGFR 2 protein levels in AML and myelodysplastic syndrome ( MDS ) , and their relationship to VEGF protein levels . ^^^ VEGFR 1 levels were significantly higher in AML than in MDS ( P = 0 . 0004 ) , but no significant difference was found in the VEGFR 2 levels ( P = 0 . 5 ) . ^^^ We found a significant correlation between VEGF and VEGFR 2 levels in both AML and MDS ( P < 0 . 0000001 andP < 0 . 0002 respectively ) but not between VEGF and VEGFR 1 levels . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
We performed immunohistochemical analysis using specific antibodies to VEGF and to the known VEGF receptors , VEGFR 1 and VEGFR 2 , on paraffin sections of human nasal polyps ( n=11 ) and controls of human mucosa of the normal middle turbinates ( n=6 ) . ^^^ VEGFR 1 and VEGFR 2 expression was faint in the healthy turbinates . ^^^ These findings support a role for VEGF and its receptors , VEGFR 1 and VEGFR 2 , in the development and perpetuation of edema and angiogenesis in nasal polyps . . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
In contrast , VEGFR 1 and VEGFR 2 expression was restricted to 6 and 2 out of 10 cell lines , respectively . ^^^ Although SU 5614 was a potent inhibitor of the VEGF induced endothelial cell sprouting in vitro , the sensitivity of leukemic cells toward the growth inhibitory activity of the compound was determined by the c kit , but not by the VEGFR 1 / 2 expression . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Since factors regulating angiogenesis in tendons are largely unknown , we analyzed the expression of the vascular endothelial growth factor ( VEGF ) and its receptors VEGFR 1 ( flt 1 ) and VEGFR 2 ( KDR ) in human fetal and adult tendon tissue by immunohistochemical , biochemical , and molecular biology methods . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Inhibition of VEGFR 1 , but not VEGFR 2 , blocked HSC cell cycling , differentiation and hematopoietic recovery after BM suppression , resulting in the demise of the treated mice . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
At different stages of tumor growth , the histological aspects were described and sections were immunostained for VEGF , Ang 2 and their receptors VEGFR 1 , VEGFR 2 and Tie 2 . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Three anti VEGF agents were tested : an anti human VEGF ( 165 ) RNA based fluoropyrimidine aptamer ; a monoclonal anti human VEGF antibody ; and VEGF Trap , a composite decoy receptor based on VEGFR 1 and VEGFR 2 fused to an Fc segment of IgG 1 . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Targeted disruption of vascular endothelial growth factor ( VEGF ) or its receptor kdr ( flk 1 , VEGFR 2 ) in mouse embryos results in a severe reduction of all blood vessels , while the complete loss of flt 1 ( VEGFR 1 ) leads to an increased number of hemangioblasts and a disorganized vasculature . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
In the capillaries , VEGFR 1 ( flt 1 ) and VEGFR 2 ( flk 1 / KDR ) were also visualized . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
At vessels growing into the hypertrophic cartilage FLT 1 ( VEGFR 1 ) and KDR ( VGEFR 2 ) could be visualized . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
OBJECTIVE : To document the pattern of expression of the two angiogenic growth factors , vascular endothelial growth factor ( VEGF ) B and VEGF C , and of their receptors , VEGFR 1 , VEGFR 2 , and VEGFR 3 , in order to investigate their possible role in the regulation of angiogenesis in normal cycling human endometrium . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Vascular permeability factor / vascular endothelial growth factor ( VPF / VEGF ) functions by activating two receptor tyrosine kinases , Flt 1 ( VEGFR 1 ) and KDR ( VEGFR 2 ) , both of which are selectively expressed on the primary vascular endothelium . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
A semiquantitative evaluation of the labeling revealed an induction of genes encoding EPAS 1 , VEGF , VEGFR 1 , VEGFR 2 , endothelin receptor , type B ( ETB ) and endothelin receptor , type A ( ETA ) in malignant pheochromocytomas as compared to benign tumors . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Vascular endothelial growth factor ( VEGF ) is a key regulator for placental angiogenesis and vascular functions via activating two high affinity tyrosine kinase receptors , VEGF receptor 1 ( VEGFR 1 ) and 2 ( VEGFR 2 ) . ^^^ Using PCR analysis , we also identified partial sequences of multiple VEGF isoforms ( VEGF 188 , 183 , 164 , and 120 ) as well as VEGFR 1 , VEGFR 2 , and neuropilin 2 ( NP 2 ) from the OFPAE cell cDNA library . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
The viral VEGFs are unique among the family in that they recognize VEGF receptor 2 ( VEGFR 2 ) but not VEGFR 1 or VEGFR 3 . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
These results suggested that greater requirement of VEGF in the maintenance than induction of ECs was due to the activity of VEGFR 1 sequestering VEGF from VEGFR 2 signal . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
VEGF A is a multifunctional cytokine that is widely expressed by tumor cells and that acts through receptors ( VEGFR 1 , VEGFR 2 , and neuropilin ) that are expressed on vascular endothelium and on some other cells . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
In contrast , its vascular receptors VEGFR 1 ( Flt 1 ) and VEGFR 2 ( Flk 1 ) , were clearly upregulated . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
As shown by reverse transcription polymerase chain reaction and immunocytochemistry , rat microglial cells as well as the murine cell line BV 2 express the VEGFR 1 , but not VEGFR 2 . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
The vascular endothelial growth factor ( VEGF ) receptor family in mammals contains three members , VEGFR 1 ( Flt 1 ) , VEGFR 2 ( KDR / Flk 1 ) and VEGFR 3 ( Flt 4 ) , which directly regulate the formation of blood vessels and lymphatic vessels . ^^^ Recently , some novel findings on the phylogenetical conservation of VEGF receptor genes in animals were reported : the conservation of the VEGFR1 / soluble VEGFR 1 gene in birds , and the conservation of the VEGFR PDGFR like receptor gene in nonvertebrates . ^^^ Based on this new information as well as established observations , here the possibility is discussed that the three VEGFR genes phylogenetically segregated not at once when the vertebrates established , but in a step wise manner : two genes first ( the VEGFR1 / R2 progenitor and the VEGFR 3 gene ) , and subsequently the three genes VEGFR 1 , R 2 and R3 . . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
The biological significance of VEGF , EGF , and bFGF is mediated by their receptors , which belong to the family of tyrosine kinase receptors : Flt 1 ( VEGFR 1 ) , KDR ( VEGFR 2 ) , EGFR , FGFR 1 , FGFR 2 , FGFR 3 , and FGFR 4 . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
However , the mRNA expression of their receptors ( VEGFR 1 = Flt 1 , VEGFR 2 = KDR and neuropilin 1 ) was recognized only in the OA samples . ^^^ The protein expression of VEGFR 1 and VEGFR 2 in OA chondrocytes was also demonstrated by immunohistochemistry of the OA cartilage tissue and cultured OA chondrocytes . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Efforts to therapeutically stimulate or inhibit vessel growth have been primarily focused on vascular endothelial growth factor ( VEGF ) and its receptor VEGFR 2 ( Flk 1 ) , while little attention has been devoted to the therapeutic potential for angiogenic disorders of placental growth factor ( PlGF ) , a VEGF family member , and its receptor VEGFR 1 ( Flt 1 ) . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
VEGF is a secreted growth factor that mediates its biological effects by binding to two transmembrane tyrosine kinase receptors , VEGFR 1 and VEGFR 2 . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
To understand the molecular basis of the biological activity of VEGF E , we have functionally mapped residues important for interaction of VEGF E with VEGFR 2 by exchanging the domains between VEGF E ( NZ 7 ) and PlGF , which binds only to VEGFR 1 ( Flt 1 ) . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
BACKGROUND : To clarify the clinical significance of vascular endothelial growth factor ( VEGF ) and its receptors , VEGFR 1 ( Flt 1 ) and VEGFR 2 ( Flk 1 ) , in esophageal cancers , we evaluated the relationships between the expression of VEGF and its receptors , tumor microvessel density ( MVD ) , clinicopathological factors and prognosis . ^^^ Although VEGF expression correlated significantly with the MVD ( p < 0 . 05 ) , the correlations between VEGFR 1 and VEGFR 2 expression and the MVD were not significant . ^^^ There were , however , significant intercorrelations in expression between VEGF , VEGFR 1 and VEGFR 2 . ^^^ However , although VEGF expression correlates significantly with coexpression of its receptors , VEGFR 1 and VEGFR 2 , these receptors do not appear to contribute directly to tumor progression . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
VEGF A binds and activates two receptors , VEGFR 1 ( Flt 1 ) and VEGFR 2 ( KDR / Flk 1 ) . ^^^ To dissect the signals of these two receptors , we generated transgenic mice overexpressing either the VEGFR 2 specific ligand VEGF E ( NZ 7 ) or VEGFR 1 specific ligand PlGF 2 under the control of the Keratin 14 promoter . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
PCPV ( VR 634 ) VEGF bound VEGF receptor 2 ( VEGFR 2 ) but did not bind VEGFR 1 or VEGFR 3 . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
By immunohistochemical staining of tumor cells from 26 patients , VEGFR 1 was detected in 24 ( 92 % ) , VEGFR 2 in five ( 19 % ) , Tie 2 in 14 ( 54 % ) , and c Met , a specific receptor of hepatocyte growth factor ( HGF ) in 23 patients ( 88 % ) . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
From the literature it is clear that overexpression of VEGFs and their receptors VEGFR 1 , VEGFR 2 and VEGFR 3 is causing increased microvascular permeability and angiogenesis in eye conditions such as DR and AMD . ^^^ The cellular distribution of VEGFR 1 , VEGFR 2 and VEGFR 3 suggests various specific functions of the VEGF family in normal retina , both in the retinal vasculature and in neuronal elements . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Therefore , we used the VEGF homologue placenta growth factor ( PlGF ) , which only binds to VEGFR 1 and VEGF E , which only recognizes VEGFR 2 . ^^^ Evaluation of collateral growth by determining collateral conductance and angiographic scores demonstrated that the VEGFR 1 specific PlGF contributed significantly more to arteriogenesis than the VEGFR 2 specific VEGF E . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
BACKGROUND AND OBJECTIVES : In tumors , vascular endothelial growth factor A ( VEGF A ) stimulates angiogenesis and vascular permeability by activating the tyrosine kinase receptor 2 ( VEGFR 2 or KDR / Flk 1 ) and 1 ( VEGFR 1 or Flt 1 ) . ^^^ CM VEGF A stimulates proliferation and chemotaxis in endothelial cells ( both being mandatory for angiogenesis ) via VEGF receptor 2 ( VEGFR 2 ) , and in residual stromal cells via the VEGFR 1 . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Among these is vascular endothelial growth factor ( VEGF ) , which acts on endothelial cells by binding with 2 specific receptors , VEGFR 1 and VEGFR 2 . ^^^ The aims of this study were to evaluate the expression of VEGF , VEGFR 1 , and VEGFR 2 in digestive endocrine tumors and to examine its correlation with MVD and malignancy . ^^^ A total of 84 specimens from endocrine neoplasms and normal gut and pancreatic tissue were immunohistochemically studied using specific antibodies directed against VEGF , VEGFR 1 , VEGFR 2 , endothelial antigens , and gastroenteropancreatic hormones . ^^^ VEGFR 1 IR cells were found in 44 of 82 tumors , and VEGFR 2 IR cells were found in 55 of 82 tumors , with no predilection for any specific tumor type . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
MATERIAL AND METHODS : We have measured the expression of angiogenesis regulating genes angiopoietin 1 ( Ang 1 ) , angiopoietin 1 ( Ang 2 ) and their receptor Tie 2 , vascular endothelial growth factor ( VEGF ) and its receptors VEGFR 1 and VEGFR 2 in sorted population of CD34+ and CD34+ / CD133+ cells from human cord blood and bone marrow , and in their differentiating progeny , using real time reverse transcriptase polymerase chain reaction . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Staining for the receptors VEGFR 1 and VEGFR 2 was positive in large lymphoid cells in four and two cases , respectively . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
VEGFR 1 and VEGFR 2 mRNA expression also increased in air exposed animals , and these , too , were significantly decreased by hyperoxia by day 9 and day 12 , respectively . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
VEGF interacts with its receptors , VEGFR 2 and VEGFR 1 , expressed on endothelial and hematopoietic stem cells , and thereby promotes recruitment of these cells to neo angiogenic sites , accelerating the revascularization process . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Treatment with neutralising anti VEGF receptor ( VEGFR ) antibodies against VEGFR 1 and VEGFR 2 suppressed proliferation of acid exposed A 549 cells but had no effect on control cells . ^^^ CONCLUSIONS : Exogenous VEGF interacts with VEGFR 1 and VEGFR 2 on the surface and regulates the proliferation of injured alveolar lining epithelial cells in an autocrine or paracrine fashion . . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
The specific action of the VEGF on the endothelial cells is mainly regulated by two types of RTK of the VEGF family , VEGFR 1 , or Flt 1 , and VEGFR 2 , or KDR / Flk 1 . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
This synergy has been hypothesized to be due to a combination of the following : signaling by PlGF through VEGFR 1 and displacement of VEGF from VEGFR 1 to VEGFR 2 by PlGF , causing increased signaling through VEGFR 2 . ^^^ We show that , whereas a significant change in the formation of endothelial surface growth factor VEGFR 1 complexes is predicted in the presence of PlGF , the increase in the number of VEGFR 2 containing signaling complexes is less significant ; these results were shown to be robust to significant variation in the kinetic parameters of the model . ^^^ Synergistic effects observed in that experiment thus appear unlikely to be due to VEGF displacement but to a shift from VEGF VEGFR 1 to PlGF VEGFR 1 complexes and an increase in total VEGFR 1 complexes . ^^^ These results suggest that VEGFR 1 signaling can be functional in adult derived endothelial cells . . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
VEGF and its receptors , the Fms like tyrosine kinase receptor ( FLT 1 , VEGFR 1 ) and the kinase insert domain containing receptor KDR ( VEGFR 2 / FLK 1 ) were detected immunohistochemically . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Placental growth factor ( PlGF ) and vascular endothelial growth factor ( VEGF ) are involved in placental angiogenesis through interactions with the VEGFR 1 and VEGFR 2 receptors . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
At microvessels , the receptors VEGFR 1 ( flt 1 ) and VEGFR 2 ( KDR ) could also be visualized . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
We measured the gene expression of VEGF ligands ( VEGF A , VEGF B , VEGF C , and VEGF D ) and their receptors ( VEGFR 1 , VEGFR 2 , and VEGFR 3 ) , in normal colorectal tissues ( n = 20 ) , adenomas ( n = 10 ) , and in CRC ( n = 71 ) representing different Duke ' s stages using ribonuclease protection assay , semi quantitative relative reverse transcriptase polymerase chain reaction , together with the pattern of their expression by immunohistochemistry . ^^^ VEGFR 1 was significantly correlated with tumour grade ( p = 0 . 02 ) , Duke ' s stage ( p < 0 . 001 ) , and lymph node involvement ( p = 0 . 004 ) , VEGFR 2 with lymph node involvement ( p = 0 . 02 ) , and VEGFR 3 did not correlate with any of the clinicopathological variables tested . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
A selective and oral small molecule inhibitor of vascular epithelial growth factor receptor ( VEGFR ) 2 and VEGFR 1 inhibits neovascularization and vascular permeability . ^^^ In biochemical assays , SU 10944 exhibits potent inhibitory activity against VEGFR 1 ; weak activity against other related subgroup members , including stem cell factor receptor ( SCFR ) , platelet derived growth factor receptor beta ( PDGFRbeta ) , and fibroblast growth factor receptor 1 ( FGFR 1 ) ; and no detectable activity against other protein tyrosine kinases such as epidermal growth factor receptor ( EGFR ) , Src , and hepatocyte growth factor receptor . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Sections of testes fixed in Bouin solution and embedded in paraffin were subjected to immunofluorescent staining with specific antibodies against VEGF , and its receptors , VEGFR 1 ( Flt 1 ) and VEGFR 2 ( Flk 1 ) . ^^^ VEGFR 1 and VEGFR 2 were not expressed by the Sertoli cell . ^^^ Although VEGFR 2 was expressed in the cytoplasm of type A spermatogonia , VEGFR 1 was expressed in the acrosomal region of spermatids and spermatozoa . ^^^ The mRNA expression of VEGFR 1 and VEGFR 2 in germ cells was inversely correlated during postnatal development of the mouse testis . ^^^ Thus , VEGF may play a potential role in regulating the initial stages of the process of spermatogonial proliferation through VEGFR 2 and spermiogenesis through VEGFR 1 . . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
The biological effects of VEGF are mediated by two receptor tyrosine kinases ( RTKs ) , VEGFR 1 and VEGFR 2 , which differ considerably in signaling properties . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Inhibition of VEGFR 1 signaling , but not VEGFR 2 ( Flk 1 , KDR ) , blocked HSC cell cycling , differentiation and hematopoietic recovery after BM suppression , resulting in the demise of the treated mice . ^^^ Plasma elevation of placental growth factor ( PlGF ) , which signals through VEGFR 1 , but not VEGFR 2 , restored hematopoiesis during the early and late phases following BM suppression . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
VEGF mediates its actions through activation of two receptor tyrosine kinases , VEGFR 1 and VEGFR 2 . ^^^ Selective stimulation of VEGFR 2 showed similar results , whereas the isolated activation of VEGFR 1 was without effect . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
To investigate the mechanisms responsible for survival of blood vessels in the developing retina , we characterized two VEGF A receptors , VEGF receptor 1 ( VEGFR 1 , also known as Flt 1 ) and VEGF receptor 2 ( VEGFR 2 , also known as Flk 1 ) . ^^^ At 5 days postpartum ( P 5 ) , VEGFR 1 protein was colocalized with retinal vessels , whereas VEGFR 2 was detected only in the neural retina . ^^^ Real time RT PCR identified a 60 fold induction of VEGFR 1 mRNA in retina from P 3 ( early vascularization ) to P 26 ( fully vascularized ) , and no significant change in VEGFR 2 mRNA expression . ^^^ Placental growth factor 1 ( PlGF 1 ) , which exclusively binds VEGFR 1 , decreased hyperoxia induced retinal vaso obliteration from 22 . 2 % to 5 . 1 % , whereas VEGF E , which exclusively binds VEGFR 2 , had no effect on blood vessel survival . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
In contrast to the conserved ability to bind human VEGFR 2 , the viral growth factors did not bind either VEGFR 1 or VEGFR 3 . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
In contrast to VEGF and its receptor VEGFR 2 , PlGF and its receptor VEGFR 1 have been largely neglected and therefore their potential for therapy has not been previously explored . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
The action of VEGF is mediated by two different high affinity receptors VEGF receptor 1 ( VEGFR 1 ; flm like tyrosine kinase 1 ; flt 1 ) and VEGFR 2 ( kinase insert domain containing receptor ; flk 1 ) predominantly located on the vascular endothelium . ^^^ RESULTS : RT PCR revealed that the expression of VEGFR 1 and 2 mRNA was upregulated between 1 h and 3 days after endotoxin instillation , with peak expression occurring at 12 h and on Day 1 . ^^^ Expression patterns of VEGFR 1 and 2 protein observed by Western blotting were similar to those of VEGFR 1 and 2 mRNA and peak expression was observed on Day 1 . ^^^ CONCLUSION : These results suggest that both VEGFR 1 and 2 are upregulated during experimental otitis media in the rat and these receptors may play different roles in the production of effusion in OME . . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Of the three VEGF receptors ( VEGFR ) , VEGFR 1 and 2 are expressed on blood vessels ; VEGFR 2 is found also on lymphatic vessels . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
We have previously demonstrated the differential expression in tumor associated blood vessels of two vascular endothelial growth factor receptors ( VEGFRs ) , VEGFR 1 and VEGFR 2 , during initiation and progression of prostate cancer in the genetically engineered transgenic adenocarcinoma of the mouse prostate ( TRAMP ) mouse model . ^^^ In our `` progression switch ' ' model , expression of VEGFR 1 is associated with early and more differentiated disease , whereas expression of VEGFR 2 is associated with advanced and more poorly differentiated disease . ^^^ In our early intervention trial , administration of SU 5416 to TRAMP mice did not appear to influence angiogenesis or tumor progression between 10 and 16 weeks of age , a time corresponding to high levels of VEGFR 1 expression . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Vascular endothelial growth factor ( VEGF ) and its receptors VEGFR 1 and VEGFR 2 have been implicated as key players in vascular remodelling and placentation . ^^^ Implantation in marmosets occurs at day 11 of pregnancy ; hence , these time points were chosen so that the peri implantation period and very early pregnancy could be studied . mRNAs for VEGF , VEGFR 1 and VEGFR 2 , angiopoietin 1 , angiopoietin 2 and their receptor Tie 2 were localized and quantified by in situ hybridization . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Hematoxylin and eosin staining , immunohistochemistry , in situ hybridization , and zymography were used to examine the expression of MMP 9 , MMP 9 mRNA , CD 34 , alpha SMA , VEGFR 1 , VEGFR 2 . ^^^ All BGC 823 cells were MMP 9 , MMP 9 mRNA , VEGFR 2 positive and VEGFR 1 negative . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
BACKGROUND : It has been shown that expression of the potent angiogenic factor , vascular endothelial growth factor ( VEGF ) , and its receptors , flt 1 ( VEGFR 1 ) and KDR / Flk 1 ( VEGFR 2 ) , increased during the development of liver fibrosis . ^^^ METHODS : A model of CCl ( 4 ) induced hepatic fibrosis was used to assess the role of VEGFR 1 and VEGFR 2 by means of specific neutralising monoclonal antibodies ( R 1mAb and R 2mAb , respectively ) . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Tumour cells are the main source of VEGF in GBMs whereas VEGF receptors ( VEGFR 1 , its soluble form sVEGFR 1 , VEGFR 2 and neuropilin 1 ) are expressed predominantly by endothelial cells . ^^^ We used quantitative RT PCR , Western blot , gelatin zymography and immunohistochemistry to study the expression of VEGF , VEGFR 1 , VEGFR 2 , sVEGFR 1 , neuropilin 1 , MT 1 MMP , MMP 2 , MMP 9 and TIMP 2 in 20 human GBMs and 5 normal brains . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Samples were analyzed for VEGF , VEGFR 1 and VEGFR 2 mRNA expression using real time PCR and protein expression using Western Blot analysis . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
DESIGN : Immunohistochemical analysis for VEGFR 1 ( n = 13 ) , VEGFR 2 ( n = 21 ) , and VEGFR 3 ( n = 16 ) was performed on human HNSCC tumor samples . ^^^ RESULTS : The HNSCC tumor cells expressed VEGFR 1 , VEGFR 2 , and VEGFR 3 in all specimens evaluated . ^^^ Staining intensity for VEGFR 1 and VEGFR 2 was significantly higher in tumor cells and macrophages than in VECs stained for the same receptor . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Therefore , VEGF and its receptors VEGFR 1 and VEGFR 2 are prime targets for anti angiogenic intervention which is thought to be one of the most promising approaches in cancer therapy . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Although VEGF A binds two receptors , VEGFR 1 and 2 , a newly isolated ligand VEGF E ( Orf virus derived VEGF ) binds and activates only VEGFR 2 . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Expression and localization of mRNAs for vascular endothelial growth factor ( VEGF ) , VEGF receptor 1 ( Flt ) and VEGF receptor 2 ( KDR ) ( VEGFR 1 and VEGFR 2 , respectively ) were investigated in pig corpora lutea . ^^^ Northern blot analysis of total RNA indicated hybridization of pig VEGF , VEGFR 1 and VEGFR 2 cDNA probes to mRNA transcripts of approximately 3 . 9 , 7 . 0 and 5 . 0 kb , respectively . ^^^ In situ hybridization revealed that VEGF mRNA was localized predominantly in large luteal cells , whereas both VEGFR 1 and VEGFR 2 were localized to small cells . ^^^ Although the expression of VEGF mRNA was unchanged during the luteal phase , variations in the expression of VEGFR 1 and VEGFR 2 mRNAs indicate that differential regulation of expression of the VEGF receptors may play a role in the control of VEGF mediated vascular growth at different phases of development and maturation of the pig corpus luteum . . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
VEGF binds to the tyrosine kinase receptors VEGFR 1 and VEGFR 2 , and loss of VEGF or its receptors results in abnormal angiogenesis and lethality during development . ^^^ However , the expression of VEGFR 1 and VEGFR 2 on non vascular cells suggests additional biological properties for these growth factors . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
To gain insight into how this bacteria induces angiogenesis in vivo , we performed in situ hybridization of clinical specimens of verruga peruana for the angiogenesis factors vascular endothelial growth factor ( VEGF ) , its receptors VEGFR 1 and VEGFR 2 , and angiopoietin 2 . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
We studied the expression of VEGF and its receptors ( VEGFR 1 or Flt 1 and VEGFR 2 or Flk 1 / KDR ) by myeloma cell lines and plasma cells isolated from patients , using different methods . ^^^ We further evaluated the expression of both VEGFR 1 and VEGFR 2 in different myeloma cell lines and five sorted myeloma bone marrow samples by RT PCR . ^^^ All the myeloma cell lines expressed VEGFR 1 and three of the cell lines expressed VEGFR 2 . ^^^ VEGFR 1 expression was detected in all and VEGFR 2 in all but one of the sorted marrow samples . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Neovascularization in the human heart is associated with expression of VEGF A and its receptors Flt 1 ( VEGFR 1 ) and KDR ( VEGFR 2 ) . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Immunohistochemical techniques were used to evaluate VEGF protein localization with rabbit polyclonal anti rat VEGF , VEGF receptor ( VEGFR ) expression with rabbit polyclonal antibodies to VEGFR 1 and 2 , microvascular density with mouse monoclonal anti rat CD 31 , and astrocytic reactivity with polyclonal anti glial fibrillary acidic protein , in cerebral cortical tissue of the right middle cerebral artery territory . ^^^ Consistent with the VEGF protein expression findings , up regulation of VEGFR 1 but not VEGFR 2 expression on endothelial cells in the model brains was observed . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Furthermore , introducing the carboxyl terminus of another receptor , i . e . , VEGFR 1 , restored the ligand dependent activation of the carboxyl terminus deleted VEGFR 2 and its ability to stimulate cell proliferation . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Addition of VEGFR 1 / Fc for the first 5 days of culture ( phase 1 ) severely inhibited the development of OCPs . ^^^ The generation of OCPs in phase 1 was resistant to signal blocking mediated by Kit receptors , but that in phase 2 was partially inhibited by either an anti Kit antagonistic antibody or VEGFR 1 / Fc and was severely inhibited by the combination of both reagents . ^^^ We thus showed that OCPs expanded in phase 2 , but the majority of OCPs arose from ES cells in a manner dependent on VEGFR 1 binding factor ( s ) in phase I . . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
STUDY DESIGN : We measured mRNA expression of vascular endothelial growth factor ( VEGF ) , angiopoietin 1 and 2 ( Ang 1 and Ang 2 ) , their receptors VEGFR 1 , VEGFR 2 , Tie 2 , fibroblast growth factor 2 ( FGF 2 ) , and its receptor FGF 2R in placental tissue of diabetes type 1 patients , in normal term placenta , and endometrium of non pregnant women by real time reverse transcriptase PCR . ^^^ We did not detect a significant difference in the expression of Ang 1 , Ang 2 , Tie 2 , VEGF , VEGFR 1 , VEGFR 2 , FGF 2 , and FGF 2R in normal and diabetes type 1 placenta . ^^^ CONCLUSION : These data show differential expression of Ang 2 , VEGFR 1 , VEGF , and VEGFR 2 in placenta and endometrium . ^^^ The expression of Ang 1 , Ang 2 , Tie 2 , VEGF , VEGFR 1 , VEGFR 2 , FGF 2 , and FGF 2R was not different in normal and type 1 diabetes placenta . . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Prognostic significance of VEGF receptors , VEGFR 1 ( Flt 1 ) and VEGFR 2 ( KDR / Flk 1 ) in breast carcinoma ] . ^^^ OBJECTIVES : The aim of this study was to investigate the prognostic value of vascular endothelial growth factor ( VEGF ) receptors , VEGFR 1 ( Flt 1 ) and VEGFR 2 ( KDR / Flk 1 ) in breast carcinoma . ^^^ VEGFR 1 and VEGFR 2 immunostained surface was evaluated in percentage of the total tumor specimen surface by light microscopy ( x 100 ) . ^^^ RESULTS : VEGFR 1 and VEGFR 2 were strongly expressed in endothelial cells within blood microvessels , and weakly in tumor cells . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
In compromised pregnancies , elevated levels of VEGF A and VEGFR 1 mRNAs may reflect the hypoxic status of the placenta . ^^^ On contrast , the membrane bound VEGFR 1 was decreased in the placental bed of preeclamptic patients . ^^^ Preeclampsia was associated with low levels of circulating PlGF and increased levels of total VEGF A and soluble VEGFR 1 . ^^^ The first one is related to an overproduction of competitive soluble VEGFR 1 that may lead to suppression of VEGF A and PlGF effects . ^^^ The second one is the down regulation of its membrane bound form ( VEGFR 1 ) in the placental bed , which may result in the defective uteroplacental development . . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
The compounds were evaluated as inhibitors of vascular endothelial growth factor receptors VEGFR 2 ( Flk 1 , KDR ) and VEGFR 1 ( Flt 1 ) ; epidermal growth factor receptor ( EGFR ) ; and platelet derived growth factor receptor beta ( PDGFR beta ) . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
The IR levels of HIF 1alpha and VEGF reached plateau at 4 weeks , and the IRs of VEGFR 1 and VEGFR 2 were strongly positive in the hypoxic group . ^^^ Yet , the expression of VEGFR 1 IR was mild , whereas the VEGFR 2 IR was intense in normoxic CBs , suggesting an upregulation of VEGFR 1 but not VEGFR 2 in hypoxia . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
The VEGFR family proteins consist of VEGFR 1 / Flt 1 , VEGFR 2 / KDR / Flk 1 , and VEGFR 3 / Flt 4 . ^^^ Consistent with its reported effects on VEGFR 2 [ IC ( 50 ) < 2 nM ] , ZM 323881 inhibited activation of VEGFR 2 , but not of VEGFR 1 , epidermal growth factor receptor ( EGFR ) , platelet derived growth factor receptor ( PDGFR ) , or hepatocyte growth factor ( HGF ) receptor . ^^^ We studied the effects of VEGF on human aortic endothelial cells ( HAECs ) , which express VEGFR 1 and VEGFR 2 , but not VEGFR 3 , in the absence or presence of ZM 323881 . ^^^ Inhibition of VEGFR 2 blocked activation of extracellular regulated kinase , p 38 , Akt , and endothelial nitric oxide synthetase ( eNOS ) by VEGF , but did not inhibit p 38 activation by the VEGFR 1 specific ligand , placental growth factor ( PIGF ) . ^^^ Inhibition of VEGFR 2 thus blocked all VEGF induced endothelial cellular responses tested , supporting that the catalytic activity of VEGFR 2 is critical for VEGF signaling and / or that VEGFR 2 may function in a heterodimer with VEGFR 1 in human vascular endothelial cells . . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
The inhibition of VEGFR 1 results in a dramatic decrease in the number of capillary connections , indicating that VEGFR 1 ligands promote branching angiogenesis . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
VEGF binds and activates two tyrosine kinase receptors , VEGFR 1 ( Flt 1 ) and VEGFR 2 ( KDR / Flk 1 ) , and stimulates endothelial cell growth , survival , and vascular permeability . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Immature B cell malignancies synthesize VEGF , VEGFR 1 ( Flt 1 ) and VEGFR 2 ( KDR ) . ^^^ We now show that transcripts of VEGF , and its receptors VEGFR 1 and VEGFR 2 are concomitantly expressed in both ALL cell lines and primary pre B ALL . ^^^ Similarly , VEGFR 1 and VEGFR 2 proteins are also detectable by FACS analysis . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
The biological activity of VEGF is mediated by two different receptor tyrosine kinases : VEGFR 2 and VEGFR 1 . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
VEGF receptors VEGFR 2 ( KDR , flk 1 ) and VEGFR 1 ( flt 1 ) could be immunostained on osteoclasts and osteoblasts . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
The aim of the present study was to investigate the expression of the VEGF receptors VEGFR 1 , VEGFR 2 , and VEGFR 3 on B CLL cells , singly and combined . ^^^ Specific receptor expression , examined in 27 samples by immunocytochemical methods , was positive for VEGFR 1 in all 27 patients and for VEGFR 2 and VEGFR 3 in 26 ( 96 % ) . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Moreover , in these sprouts VEGFR 2 and VEGFR 3 expression was very high in endothelial cells , whereas many pericytes were present that were positive for PDGFR beta , VEGFR 1 , and NG 2 proteoglycan and negative for alpha SMA . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Placental growth factor , which binds to VEGFR 1 but not VEGFR 2 , does not promote capillary sprouting . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
VEGF 164 ( 165 ) as the pathological isoform : differential leukocyte and endothelial responses through VEGFR 1 and VEGFR 2 . ^^^ VEGF ( 164 ) was also more potent at inducing the chemotaxis of monocytes , an effect that was mediated by VEGFR 1 . ^^^ In an immortalized human leukocyte cell line , VEGF ( 165 ) was found to induce tyrosine phosphorylation of VEGFR 1 more efficiently . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
METHODS : Expression of VEGF and its receptors ( VEGFR 1 and VEGFR 2 ) was examined in passive Heymann nephritis ( PHN ) and puromycin aminonucleoside nephrosis ( PAN ) , by immunohistochemistry , in situ hybridization , Northern and Western blotting . ^^^ Increased expression of VEGFR 1 and VEGFR 2 protein was seen in glomerular endothelial cells of PHN and PAN rats by immunohistochemistry , as was VEGFR 2 mRNA by in situ hybridization . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Immunohistochemical analysis of the cultures demonstrated the presence of VEGFR 2 after VEGF treatment , as well as the expression of the VEGF receptor VEGFR 1 ( flt 1 ) . ^^^ Treatment of the cultures with antisense oligonucleotides against VEGFR 2 , but not against VEGFR 1 , abolished the effect of VEGF on the length of Map 2 ( + ) processes . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Vascular endothelial growth factor and its soluble receptors VEGFR 1 and VEGFR 2 in the serum of patients with systemic lupus erythematosus . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Two distinct receptors for VEGF , the tyrosine kinase receptors VEGFR 1 ( Flt 1 ) and VEGFR 2 ( Flk 1 / KDR ) , have been identified . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
These findings suggest that both VEGFR 1 and VEGFR 2 are involved in stem cell mobilization . . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
In contrast , VEGF A binds VEGFR 1 and VEGFR 2 and is an essential hemangiogenic factor . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Combined systemic treatment with blocking antibodies against VEGF receptor 1 ( VEGFR 1 ) and VEGFR 2 potently inhibited inflammation and also decreased lymphatic vessel size . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
We demonstrate here that human lymphoma cells secrete vascular endothelial growth factor ( VEGF ) and express VEGF receptor 1 ( VEGFR 1 ) and VEGFR 2 . ^^^ Proliferation of non Hodgkin lymphoma ( NHL ) cells under serum free conditions was enhanced by the addition of VEGF and was blocked by VEGFR 1 and VEGFR 2 specific antibodies . ^^^ To differentiate between VEGF mediated autocrine and paracrine effects on lymphoma growth , NOD / SCID mice engrafted with human diffuse large B cell lymphoma ( DLBCL ) were treated with species specific antibodies against human VEGFR 1 ( 6 . 12 ) , human VEGFR 2 ( IMC 1C11 ) , murine VEGFR 1 ( MF 1 ) , or murine VEGFR 2 ( DC 101 ) . ^^^ Treatment with 6 . 12 or DC 101 ( targeting tumor VEGFR 1 and host VEGFR 2 ) reduced established DLBCL xenograft growth , whereas treatment with IMC 1C11 or MF 1 ( targeting tumor VEGFR 1 and host VEGFR 1 ) had no effect . ^^^ Decreased tumor volumes after 6 . 12 and DC 101 treatment correlated with increased tumor apoptosis and reduced vascularization , respectively , supporting the presence of autocrine VEGFR 1 and paracrine VEGFR 2 mediated pathways in lymphomagenesis . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Biweekly treatment with vehicle or the VEGF Trap , a high affinity soluble decoy receptor incorporating regions of VEGFR 1 and VEGFR 2 , was begun 1 week later ( 100 or 500 micrograms / dose , n=20 in each group ) . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
The tyrosine kinases Flt 1 ( VEGFR 1 ) and Flk 1 / KDR ( VEGFR 2 ) are high affinity VEGF receptors . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Neuropilin 1 ( Nrp 1 ) and Neuropilin 2 ( Nrp 2 ) are nonkinase vascular endothelial growth factor receptors ( VEGFR ) identified in several vertebrates , which function as coreceptors for the receptor tyrosine kinases VEGFR 1 and VEGFR 2 . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
In the present study the expression patterns of VEGFR 1 , 2 and 3 were investigated in the spinal cord of control and both sporadic and familial amyotrophic lateral sclerosis ( ALS ) patients . ^^^ Immunocytochemical analysis of control human spinal cord demonstrated that VEGFR 1 , but not VEGFR 2 or 3 was found to be present in blood vessels of both white and grey matter . ^^^ In particular , VEGFR 1 expression was increased in reactive astroglial cells in both grey ( ventral horn ) and white matter of ALS spinal cord . ^^^ In addition to the astroglial labelling , increased expression of VEGFR 1 and , to a less extent also of VEGFR 2 , was observed in blood vessels of the ALS spinal cord . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
VEGF binds VEGFR 1 as well , but the importance of VEGFR 1 signaling in vascular permeability has been largely neglected . ^^^ TfsvVEGF bound VEGFR 1 and induced its autophosphorylation to almost the same extent as VEGF ( 165 ) , but bound VEGFR 2 weakly and induced its autophosphorylation almost 10 fold less effectively than VEGF ( 165 ) . ^^^ This unique binding affinity for VEGFR 1 and VEGFR 2 leads to the vascular permeability dominant activity of TfsvVEGF . ^^^ These results suggest that Habu snakes have acquired a highly purposive molecule for a toxin , which enhances the toxicity in envenomation without inducing effective angiogenesis and the following regeneration of damaged tissues , taking advantage of the difference in signaling properties involving VEGFR 1 and VEGFR 2 between vascular permeability and angiogenesis . ^^^ TfsvVEGF is thus a potent inducing factor selective for vascular permeability through preferential signaling via VEGFR 1 . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Regulation of VEGF A , VEGFR 1 , thrombospondin 1 , 2 , and 3 expression in a human pituitary cell line ( HP 75 ) by TGFbeta 1 , bFGF , and EGF . ^^^ We used the human pituitary cell line ( HP 75 ) to examine the effects of the growth factors TGFbeta 1 , bFGF , and EGF on cell growth , and on the regulation of the pro angiogenic growth factor VEGF A and the VEGFR 1 and the anti angiogenic molecules thrombospondin ( TSP ) TSP 1 and TSP 2 along with TSP 3 . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
METHODS : The tissue samples of 42 primary ovarian epithelial carcinoma , 29 benign ovarian tumor and 11 normal ovarian tissue were used to determine the expression of VEGF , VEGFR 1 , VEGFR 2 , P STAT 1 , P STAT 3 , P STAT 5 and P STAT 6 proteins by immunohistochemical staining . ^^^ In endothelial cells , the expression of VEGFR 1 and P STAT 5 closely correlated with each other , as well as VEGFR 2 and P STAT 3 . ^^^ However , in ovarian carcinoma cells , expressions of VEGF , VEGFR 1 and VEGFR 2 were significantly correlated with P STAT 3 and P STAT 5 , but not with P STAT 1 and P STAT 6 . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
However , the presence of FGF 2 in the medium up regulated the expression of both VEGF receptors ( VEGFR 1 and VEGFR 2 ) , whereas oestradiol or VEGF treatment showed no effect on the expression of these receptors . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Angiopoietins ( Ang 1 and Ang 2 ) modulate the activity of the endothelial cell ( EC ) specific receptor tyrosine kinase Tie 2 , which together with vascular endothelial growth factor ( VEGF A ) and its EC specific receptors , VEGFR 1 and VEGFR 2 , regulate normal physiological vessel development . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
VEGFR 2 is expressed at a much higher level than VEGFR 1 under normal conditions , and both receptors are upregulated under hypoxia . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
The expression of both VEGF receptor 1 ( VEGFR 1 ) and VEGFR 2 was upregulated both in the satellite cells of the damaged muscles and during myotube formation in vitro ; the VEGF effect was mediated by the VEGFR 2 , since the transfer of PlGF , a VEGF family member interacting with the VEGFR 1 , was ineffective . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
These receptors include VEGFR 1 , VEGFR 2 , VEGFR 3 , neuropilin 1 ( NRP 1 ) , and NRP 2 . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
The mRNA and protein levels of NRP 1 , NRP 2 , VEGFR 1 , VEGFR 2 , and VEGFR 3 were semiquantitatively determined by real time PCR and Western blot , respectively . ^^^ RESULTS : In PBS treated HUVECs , mRNA levels of NRPs and VEGFRs were NRP 1 ( 0 . 013 ) , NRP 2 ( 0 . 007 ) , VEGFR 2 ( 0 . 006 ) , VEGFR 1 ( 0 . 0024 ) , and VEGFR 3 ( 0 . 0009 ) . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Compared with normal mice , 1 year old ApoE ( / ) mice fed on a high fat diet ( HFD ) had about 30 % less myocardial capillary ( P < 0 . 001 ) and arteriolar ( P < 0 . 03 ) densities , associated with decreased VEGF ( 55 % ) , VEGFR 1 ( 56 % ) and VEGFR 2 ( 78 % ) mRNA expressions and myocardial endothelial nitric oxide synthase ( eNOS ) production ( 58 % ) . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Frequently vascular endothelial cell growth factors ( VEGFs ) are mainly responsible for the pathological neovascularization as in the case in neovascularization induced by CpG oligodeoxynucleotides and herpes simplex virus infection in this report . siRNAs targeting either VEGFA , VEGFR 1 , VEGFR 2 , or a mix of the three were shown to significantly inhibit neovascularization induced by CpG when given locally or systemically . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Vascular endothelial growth factor ( VEGF ) regulates vasculogenesis and angiogenesis by using two tyrosine kinase receptors , VEGFR 1 and VEGFR 2 . ^^^ In VEGFR 1 ( TM TK ) ( / ) mice with growth arrest , membrane targeted VEGF was reduced , resulting in the suppression of VEGFR 2 phosphorylation . ^^^ Furthermore , the embryonic lethality in VEGFR 1 ( TM TK ) ( / ) mice was significantly increased to 80 to 90 % when the genotype of VEGFR 2 was changed from homozygous ( + / + ) to heterozygous ( + / ) in 129 / C57BL6 mice . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Vascular endothelial growth factor ( VEGF ) signaling is likely involved in this migration given the partial disturbance of VEGF receptor ( VEGFR ) positive cell accumulation and migration in VEGFR 2 null mice or mice with a truncated VEGFR 1 . ^^^ We show here that the expression of VEGF A is dominant in the anterior portion of the embryo , whereas VEGFR 1 and VEGFR 2 are expressed in the posterior portion of the embryo . ^^^ In addition , VEGFR positive cells migrated toward a VEGFR 1 or VEGFR 2 specific ligand in vitro . ^^^ Furthermore , VEGFR positive cells derived from wild type or VEGFR 2 ( + / ) mice moved rapidly anteriorly , whereas cells derived from VEGFR 2 ( + / ) mice carrying a truncated VEGFR 1 [ VEGFR 1 ( TM TK ) ( / ) ] migrated little when injected into wild type mice . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Exposure of wild type , VEGFR 1 ( / ) , or VEGFR 2 ( / ) embryonic stem cells to exogenous VEGF or the VEGFR 1 specific ligand , placental growth factor , revealed distinct roles of VEGF receptors . ^^^ VEGFR 1 is the primary mediator of the VEGF inhibition of DC maturation , whereas VEGFR 2 tyrosine kinase signaling is essential for early hemopoietic differentiation , but only marginally affects final DC maturation . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Although much is known about the biology of vascular endothelial growth factor ( VEGF ) and its cognate receptors ( VEGFRs ) , VEGFR 1 and VEGFR 2 , little is known about the roles of the VEGFRs neuropilin ( NP ) 1 and NP 2 in the primate endometrium . ^^^ A recent study showed that blockade of VEGF action can inhibit luminal epithelial cell proliferation , but there is no evidence of VEGFR 1 and VEGFR 2 expression in these cells . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
VEGF activity is mediated by two receptors , VEGFR 1 ( Flt 1 ) and VEGFR 2 ( Flk 1 / KDR ) , which are expressed primarily in vascular endothelial cells . ^^^ Reduced cycle , specific primer , reverse transcriptase polymerase chain reaction was performed to determine the expression of VEGF and its receptors , VEGFR 1 and VEGFR 2 . ^^^ VEGFR 1 and VEGFR 2 expression increased over 2 fold during normal skin development from E 17 to E 21 . ^^^ VEGF and its receptors , VEGFR 1 and VEGFR 2 , increase expression during skin development and dermal differentiation . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Approximately 85 % of vessels within the implants are lined by Bcl 2 positive human ECs expressing VEGFR 1 , VEGFR 2 , and Tie 2 , but not integrin alpha ( 5 ) beta ( 3 ) . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
However , it is unknown whether VEGF A ( 165 ) is mediating PGI ( 2 ) synthesis through VEGF receptor 1 ( VEGFR 1 ) and / or VEGF receptor 2 ( VEGFR 2 ) activation and whether the coreceptor NRP 1 potentiates VEGF A ( 165 ) activity . ^^^ Treatment of BAEC with VEGF analogs , VEGF A ( 165 ) ( VEGFR 1 , VEGFR 2 and NRP 1 agonist ) and VEGF A ( 121 ) ( VEGFR 1 and VEGFR 2 agonist ) ( up to 10 ( 9 ) m ) , increased PGI ( 2 ) synthesis by 70 and 40 fold within 15 min . ^^^ Treatment with VEGFR 1 ( placental growth factor and VEGF B ) or VEGFR 2 ( VEGF C ) agonist did not increase PGI ( 2 ) synthesis . ^^^ The combination of VEGFR 1 and VEGFR 2 agonists did not increase PGI ( 2 ) release . ^^^ Pretreatment with a VEGFR 2 inhibitor abrogated PGI ( 2 ) release mediated by VEGF A ( 165 ) and VEGF A ( 121 ) , and pretreatment of BAEC with antisense oligomers targeting VEGFR 1 or VEGFR 2 mRNA reduced PGI ( 2 ) synthesis mediated by VEGF A ( 165 ) and VEGF A ( 121 ) up to 79 % . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
VEGFR 1 mRNA expression increased 2 h after exercise , and neuropilin 1 expression was transiently reduced , while all three receptors increased by 6 h . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Association between intratumoral free and total VEGF , soluble VEGFR 1 , VEGFR 2 and prognosis in breast cancer . ^^^ Vascular endothelial growth factor ( VEGF ) receptors consist of three cell membrane type receptors ( VEGFR 1 , VEGFR 2 and VEGFR 3 ) , and soluble form of VEGFR 1 ( sVEGFR 1 ) , an intrinsic negative counterpart of the VEGF . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Vascular endothelial growth factor ( VEGF ) and its receptors VEGFR 1 ( Flt 1 ) and VEGFR 2 ( KDR / Flk 1 ) could play a role in tissue remodelling and angiogenesis in COPD . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
The actions of VEGF are mediated through receptors possessing tyrosine kinase activity : VEGFR 1 ( Flt 1 ) , VEGFR 2 ( Kdr / Flk 1 ) and VEGFR 3 ( Flt 4 ) . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
We characterized , at the mRNA and protein levels , the expression of VEGF A and VEGF D and their cognate receptors , VEGFR 1 , VEGFR 2 , and VEGFR 3 in early and advanced stage prostate cancer specimens . ^^^ VEGFR 1 and , to a limited extent , VEGFR 2 were activated in early stage specimens , whereas VEGFR 2 and VEGFR 3 were activated in advanced stage specimens . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
BACKGROUND AND AIMS : Recombinant erythropoietin upregulates the expression of the vascular endothelial growth factor ( VEGF ) receptors , Flt 1 ( VEGFR 1 ) and KDR / Flk 1 ( VEGFR 2 ) , in endothelial cells . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Crotalid venom vascular endothelial growth factors has preferential affinity for VEGFR 1 . ^^^ Relative to human VEGF 165 , the binding affinity of Pm VEGF to the VEGFR 1 was 1 . 7 fold higher while affinity to the VEGFR 2 was 17 fold lower . ^^^ It also stimulated tissue factor production and human monocyte chemotaxis , in accord with its specificity for VEGFR 1 . ^^^ Structural comparison among VEGF proteins from various viper venoms revealed that the two subfamilies of vipers ( Crotalinae and Viperinae ) have evolved with distinct receptor specificities for VEGFR 1 and VEGFR 2 , respectively . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
To determine the individual roles of VEGF receptors ( VEGFR ) 2 and 1 on postnatal lung growth , neonatal mice were treated with neutralizing antibodies to VEGFR 2 ( DC 101 ) or VEGFR 1 ( MF 1 ) in the perinatal period . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
No significant difference was detected in retinal VEGFR 2 levels between the two groups , whereas retinal VEGFR 1 levels in the telmisartan treated group were significantly ( P < 0 . 05 ) lower than in the vehicle treated group . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Vessel regression is followed by down regulation of expression of both VEGFR 2 and VEGFR 1 on endothelial cells and increased association of alpha smooth muscle actin positive cells with small vessels indicating their normalization , which was further supported by a regular ultrastructure . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Vascular endothelial growth factor ( VEGF ) , its receptors ( VEGFR 1 , VEGFR 2 ) and neuropillin 1 ( NRP 1 ) are expressed at variable levels in bone marrow . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Comparison of the prognosis indication of VEGFR 1 and VEGFR 2 and Tie 2 receptor expression in breast carcinoma . ^^^ The aim of this study was to investigate the prognostic value of vascular endothelial growth factor ( VEGF ) receptors , VEGFR 1 ( Flt 1 ) and VEGFR 2 ( KDR / Flk 1 ) , and Tie2 / tek receptor tyrosine kinase in breast carcinoma . ^^^ VEGFR 1 and VEGFR 2 and Tie 2 were correlated with long term ( median , 11 . 3 years ) patients ' outcome . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
LSAB immunohistochemical staining was used to determine the expression of VEGF , VEGFR 1 , VEGFR 2 and activated STATS ( P STAT 1 , P STAT 3 , P STAT 5 , P STAT 6 ) proteins . ^^^ Expressions of VEGFR 1 and VEGFR 2 were significantly elevated in OVCA , including tumor cells and stromal vascular endothelial cells ( P < 0 . 01 , compared with OVBT and NOV ) . ^^^ The staining of P STAT 1 and P STAT 6 was weak with no significant differences among OVCA , OVBT and NOV . ( 3 ) Expressions of VEGFR 1 and VEGFR 2 in endothelial cells were significantly correlated with P STAT 5 and P STAT 3 , respectively ( P = 0 . 006 and 0 . 001 ) . ^^^ In cancer cells , VEGF , VEGFR 1 and VEGFR 2 were all significantly correlated with P STAT 3 and P STAT 5 ( P = 0 . 000 ) , but not with P STAT 1 or P STAT 6 . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Expression of VEGF and the VEGF receptors VEGFR 1 ( Flt 1 ) and VEGFR 2 ( Flk 1 ) were studied by in situ hybridization and immunohistochemistry . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
It has been shown that the interaction between the potent angiogenic factor ; the vascular endothelial growth factor ( VEGF ) and its receptors ( VEGFR 1 and VEGFR 2 ) , plays a pivotal role in tumor development , including hepatocellular carcinoma ( HCC ) . ^^^ We examined the signaling cascades of VEGFR 1 and VEGFR 2 in the VEGF mediated HCC development in combination with a retroviral tetracycline ( tet ) regulated ( Retro Tet ) gene expression system , which can manipulate the gene expression in vivo by providing tet in the drinking water , as well as VEGFR 1 and VEGFR 2 specific neutralizing monoclonal antibodies ( R 1mAb and R 2mAb , respectively ) . ^^^ These results suggested that both VEGFR 1 and VEGFR 2 play important roles , and lie in the different signaling cascades by which VEGF augments HCC development and angiogenesis . . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
In this study , we first examined the expression of VEGF family ( VEGF A , B , C and D ) , VEGF A isoforms ( VEGF 120 , 164 , 188 ) , and VEGF specific receptors ( VEGFR 1 : Flt 1 ; VEGFR 2 : Flk 1 and VEGFR 3 : Flt 4 ) by quantitative RT PCR in lungs from young and old mice . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
VEGFR 1 expression was detected in the cytoplasm and the nuclei of proliferating multiple myeloma cells . ^^^ Inhibition of VEGFR 1 abrogated multiple myeloma cell proliferation and motility , suggesting that the functional interaction of VEGF A with its cognate receptor is essential for the growth of primary multiple myeloma cells . ^^^ Collectively , our results suggest that stromal dependent paracrine and intracrine VEGF A / VEGFR 1 signaling contributes to human primary multiple myeloma cell growth and therefore , VEGFR 1 blockade is a potential therapeutic strategy for the treatment of multiple myeloma . . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Although mRNA for VEGF 121 , VEGF 165 , VEGFR 1 and NRP 2 was widely expressed , mRNA expression for VEGF 189 , VEGFR 2 and NRP 1 was linked to a malignant phenotype such as local invasion . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
OBJECTIVE : To study the distribution of isoforms of vascular endothelial growth factor ( VEGF ) and its receptors VEGFR 1 and VEGFR 2 in pterygia and to compare it with that in healthy conjunctivas . ^^^ MAIN OUTCOME PARAMETERS : Vascular endothelial growth factor and VEGFR 1 and VEGFR 2 were analyzed to indentify the splice variants of VEGF as well as the distribution and amount of VEGF and both receptors in pterygia and the control tissues . ^^^ In addition to VEGF , VEGFR 1 and VEGFR 2 were detected in pterygia and conjunctivas and immunostained within the epithelium of pterygia and conjunctivas and on intrapterygial and intraconjunctival endothelial cells . ^^^ Levels of VEGFR 1 and VEGFR 2 mRNA were lower in pterygia than in conjunctivas but similar in limbal and pterygium samples . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
It is shown that bevacizumab potently neutralizes VEGF and blocks its signal transduction through both the VEGFR 1 and VEGFR 2 receptors , as demonstrated by the inhibition of VEGF induced cell proliferation , survival , permeability , nitric oxide production , as well as migration and tissue factor production . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
The mRNA species analyzed include VEGF and its receptors , VEGFR 1 and VEGFR 2 , and ANG 1 , ANG 2 and their receptor , Tie 2 . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
INTRODUCTION : Vascular endothelial growth factor ( VEGF ) and its receptors VEGFR 1 and 2 are considered to play a major in tumor angiogenesis , which is a prerequisite for growth of solid tumors . ^^^ With few exceptions , in particular oligodendrogliomas , VEGFR 1 expression was parallel to VEGFR 2 expression . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
The tyrosine kinases Flt 1 ( VEGFR 1 ) and Flk 1 / KDR ( VEGFR 2 ) are high affinity VEGF receptors . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
There are nine proteins in the immediate VEGF family : VEGFA , VEGFB , VEGFC , VEGFD , VEGF 3 , placental growth factor ( PGF ) , VEGF receptor ( VEGFR ) 1 , VEGFR 2 , and VEGFR 1 related . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
We used EA . hy 926 cells ( endothelial cells ) exposed to SM / SB to evaluate the effect on VEGFR 1 ( Flt 1 ) and VEGFR 2 ( KDR ) , with 1 step , real time quantitative reverse transcription polymerase chain reaction ( RT PCR ) . ^^^ CONCLUSION : We conclude that anti angiogenic effects of SM / SB are associated with the up regulation of VEGFR 1 ( Flt 1 ) gene expression and that they are good candidates for combination therapy to treat colorectal cancer . . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Quantitative realtime PCR revealed the expression of VEGFR 1 ( Flt 1 ) , VEGFR 2 ( KDR / Flk 1 ) , and VEGFR 3 ( Flt 4 ) , which markedly declined during osteogenic differentiation . ^^^ In an in vitro kinase assay , we could demonstrate activation of VEGFR 1 and VEGFR 2 upon stimulation with specific ligands . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
VEGF receptor 2 ( VEGFR 2 ) is mainly responsible for the dilator response to VEGF through nitric oxide ( NO ) release , whereas VEGFR 1 may sequestrate the ligand . ^^^ The expression of VEGFR 1 , VEGFR 2 , and endothelial NO synthase in pulmonary artery was measured in the same study groups using Western blot analysis . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
We now report that normal human melanocytes ( Mc ) maintained in serum free , hormone , and growth factor supplemented medium lacking phorbol ester and choleragen constitutively express VEGF receptor 1 ( VEGFR 1 ) , VEGFR 2 , and neuropilin 1 . ^^^ Prolonged culture ( > 8 weeks ) in the presence of phorbol ester abrogates VEGFR 2 expression , explaining previous reports that Mc do not express VEGFR 1 and VEGFR 2 . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
This current opinion will focus on the temporal and spatial expression of VEGF and its receptors VEGFR 1 and VEGFR 2 , and the angiopoietin receptors Tie 1 and Tie 2 in parallel to vascular maturation in human placental villi during very early stages of placental development . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
OBJECTIVE : To examine the expression of vascular endothelial growth factor ( VEGF ) and its receptors ( VEGFR 1 , VEGFR 2 ) in transdifferentiated human proximal tubular epithelial ( HK 2 ) cell induced by transforming growth factor beta 1 ( TGFbeta 1 ) . ^^^ The cellular VEGF , VEGFR 1 , and VEGFR 2 were measured with Western blot . ^^^ Meanwhile , Protein levels of VEGFR 1 and VEGFR 2 were upregulated by TGFbeta 1 in concentration and time dependent manners . ^^^ CONCLUSIONS : Increased expression of VEGFR 1 and VEGFR 2 and two phase change in VEGF expression occurred in the process of tubular epithelial transdifferentiation induced by TGFbeta 1 . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
The VEGF receptors VEGFR 1 ( flt 1 ) and VEGFR 2 ( KDR ) , typically present on endothelial cells , have also been identified in human glioblastoma tissues and cell lines . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
These phenotypes depend strongly on p110gamma rather than on p85alpha and were associated with decreased expression of Ang 1 , VCAM 1 , connexin 40 , and ephrinB 2 but increased expression of Ang 2 , VEGF A , VEGFR 1 , and VEGFR 2 . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
VEGF and its receptors , VEGFR 1 and VEGFR 2 , have been found to be expressed on subsets of normal and malignant hemopoietic cells , but the role of the individual receptors in hemopoiesis requires further study . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Vascular endothelial growth factor ( VEGF ) is a potent signalling molecule that acts through two tyrosine kinase receptors , VEGFR 1 and VEGFR 2 . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Plasma levels of vascular endothelial growth factor ( VEGF ) , basic fibroblast growth factor ( bFGF ) , interleukin 1 receptor alpha ( IL 1Ralpha ) , IL 6 , IL 8 , VEGF receptors VEGFR 1 and VEGFR 2 , and thrombopoietin ( TPO ) were measured in plasma samples of 95 patients by enzyme linked immunosorbent assay ( ELISA ) . ^^^ In a univariate Cox proportional hazards model , higher levels of IL 1Ralpha , IL 8 , VEGFR 1 , and VEGFR 2 were predictive of poor survival . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
VEGFR 1 and VEGFR 2 : two non identical twins with a unique physiognomy . ^^^ VEGFR 1 and VEGFR 2 are closely related receptor tyrosine kinases and have both common and specific ligands . ^^^ VEGFR 1 is a kinase impaired RTK whereas VEGFR 2 is a highly active kinase . ^^^ Despite their differential kinase activation potentials , both VEGFR 1 and VEGFR 2 are required for normal development and angiogenesis . ^^^ VEGFR 1 regulates angiogenesis by mechanisms that involve ligand trapping , receptor homo and heterodimerization . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Using real time PCR , we found that VEGF , at 20 ng / ml , significantly increased the expression of VEGFR 1 and VEGFR 2 by approximately 4 fold and 31 fold , respectively , compared to untreated control ( p < 0 . 05 ) . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
OBJECTIVE : To analyze the expression of vascular endothelial growth factor A ( VEGF A ) and receptors ( VEGFR 1 and VEGFR 2 ) in endometrial blood vessels , as well as microvascular density ( MVD ) , in endometrial biopsy samples from idiopathic menorrhagia patients . ^^^ Endometrial biopsy samples were used for immunohistochemistry assessments and image analysis of stained endothelial structures for VEGF A , VEGFR 1 , VEGFR 2 , and CD 34 . ^^^ MAIN OUTCOME MEASURE ( S ) : Appearance of the endometrial vascular immunoreactivity for VEGF A , VEGFR 1 , and VEGFR 2 , MVD and computer assisted stereological analysis of immunoassayed blood vessels . ^^^ RESULT ( S ) : Although the MVD did not differ between patients and controls , we observed that vascular expression of VEGF A , VEGFR 1 , and VEGFR 2 in capillaries was 1 . 8 fold , 1 . 8 fold , and 2 . 0 fold higher , respectively , in the menorrhagia group when assessed as the number of stained capillaries per unit area . ^^^ CONCLUSION ( S ) : Up regulation of VEGF A and receptors VEGFR 1 and VEGFR 2 in capillaries in menorrhagia could be involved in abnormal endometrial vascular structure and permeability . . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
VEGF receptor 1 ( VEGFR 1 / Flt 1 ) is a high affinity tyrosine kinase ( TK ) receptor for VEGF and regulates angiogenesis as well as monocyte / macrophage functions . ^^^ To examine which VEGFR is important and to clarify how colony stimulating factor 1 ( CSF 1 ) and VEGF signals interact in osteoclastogenesis , we introduced a VEGFR 1 signaling deficiency ( Flt 1 ( TK ) / ) into op / op mice . ^^^ These results strongly suggest that the interaction of signals by means of VEGFR 1 and the CSF 1 receptor plays a predominant role not only in osteoclastogenesis but also in the maintenance of bone marrow functions . . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Expression of VEGF and VEGF receptors , VEGFR 1 and VEGFR 2 , was also examined by RT PCR . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Vascular endothelial growth factor A ( VEGF A ) and its 2 transmembrane tyrosine kinase receptors , VEGFR 1 and VEGFR 2 , constitute a ligand receptor signaling system that is crucial for developmental angiogenesis . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Because prostate cancer metastasis involves hematogenous and lymphatic routes , we also evaluated expression of the vascular endothelial growth factor ( VEGF ) and receptors ( VEGFR 1 , VEGFR 2 , and VEGFR 3 ) in the metastatic deposit by immunohistochemistry . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
VEGF binds two major VEGF receptors : VEGFR 1 ( flt 1 ) and VEGFR 2 ( flk 1 / KDR ) . ^^^ Next , we treated mice with specific neutralizing monoclonal antibodies against murine VEGFR 1 and VEGFR 2 and examined their effect on the development of acute allograft rejection by histology and by analysis of graft survival . ^^^ RESULTS : The expression of VEGF , VEGFR 1 and VEGFR 2 were significantly up regulated during allograft rejection as compared to isografts . ^^^ Administration of either anti VEGFR 1 or anti VEGFR 2 alone failed to inhibit allograft rejection . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Inhibition of VEGFR 2 but not VEGFR 1 markedly disrupted angiogenic switching , persistent angiogenesis , and initial tumor growth . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Effects of oestradiol and tamoxifen on VEGF , soluble VEGFR 1 , and VEGFR 2 in breast cancer and endothelial cells . ^^^ Vascular endothelial growth factor ( VEGF ) , acting via the receptors VEGFR 1 and VEGFR 2 , is a key mediator of tumour angiogenesis . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
By immunohistochemistry and reverse transcriptase polymerase chain reaction , expression of VEGF receptor 1 ( VEGFR 1 ) , but not VEGFR 2 , was detected in murine keratinocytes during wound repair and in normal human epidermal keratinocytes ( NHEKs ) . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Local VEGF , VEGFR 1 , VEGFR 2 , syndecan , and FGFR 1 levels were 16 75 % upregulated after Ad PR 39 injections as assessed by real time PCR , suggesting upregulation of VEGF and FGF pathways . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
METHODS : We examined VEGF , VEGFR 1 and VEGFR 2 expression on 34 papillary thyroid carcinomas ( PTCs ) , 18 follicular thyroid carcinomas ( FTCs ) , eight poorly differentiated thyroid carcinomas ( PDTCs ) and on a thyroid tumour derived cell line ( NPA ' 87 ) by immunohistochemistry , reverse transcriptase PCR , immunofluorescence and Western blotting . ^^^ Similarly , 76 % of PTCs , 83 % of FTCs and 25 % of PDTCs expressed VEGFR 1 , whereas 68 % of PTCs , 56 % of FTCs and 37 % of PDTCs expressed VEGFR 2 . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Localization of vascular endothelial growth factor ( VEGF ) and its receptors VEGFR 1 and VEGFR 2 in bovine placentomes from implantation until term . ^^^ Interactions of vascular endothelial growth factor ( VEGF ) with its receptors VEGFR 1 and VEGFR 2 promoting angiogenesis have been described in placentation of human , mink and pig . ^^^ VEGFR 1 and VEGFR 2 were co localized in uterine epithelium and trophoblast as well as in blood vessel tissue and uterine glands . ^^^ The presence of VEGF , VEGFR 1 and VEGFR 2 at the feto maternal interface and in vasculature indicates that in the bovine VEGF may have ( 1 ) classic functions in angiogenesis and vascular permeability , ( 2 ) growth factor properties , facilitating feto maternal exchange via paracrine action , ( 3 ) chemotactic activity on capillary endothelium , and ( 4 ) an autocrine influence on TGC migratory activity . . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Assays for the following receptors VEGFR 1 ( Flt 1 ) , VEGFR 2 ( KDR / Flk 1 ) and PDGFR beta were performed by immunoprecipitation , while PLCgamma 1 , Akt 1 / 2 , and phospholamban B phosphorylation were assessed with Western immunoblotting . ^^^ The presence of the receptors VEGFR 1 , VEGFR 2 , and PDGFR beta was confirmed in all experimental groups . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
The vascular endothelial growth factor A ( VEGF A ) is a cytokine that promotes angiogenesis through the activation of two tyrosine kinase receptors , VEGFR 1 and VEGFR 2 , on vascular endothelial cells . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Differential regulation of vascular endothelial growth factor receptors ( VEGFR ) revealed by RNA interference : interactions of VEGFR 1 and VEGFR 2 in endothelial cell signaling . ^^^ VEGFR 2 specific siRNA had no effect on the abundance of VEGFR 1 protein . ^^^ By contrast , VEGFR 1 specific siRNA markedly not only downregulated the abundance of VEGFR 1 but also significantly reduced VEGFR 2 protein and mRNA abundance . ^^^ VEGFR 1 siRNA had no effect on the stability of VEGFR 2 protein or mRNA . ^^^ However , VEGFR 1 siRNA significantly inhibited VEGFR 2 promoter activity , as determined in luciferase assays using VEGFR 2 promoter fusion constructs in transfected BAEC . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Levels of VEGF , VEGFR 1 and VEGFR 2 were determined by enzyme immunoassay in tumor and adjacent normal tissue samples from 39 breast cancer patients . ^^^ Direct correlations were established between VEGF and VEGFR 1 , on the one hand , and VEGF and VEGFR 2 , on the other . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Unlike VEGF A , which binds to the two receptor tyrosine kinases VEGFR 1 ( Flt 1 ) and VEGFR 2 ( Flk 1 / KDR ) , VEGF B binds only to VEGFR 1 and the functional significance of VEGFR 1 signalling has remained problematic . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
This effect was dependent on the binding of VEGF ( 165 ) to VEGFR 2 , as blocking antibodies to VEGFR 2 , but not VEGFR 1 , inhibited VEGF ( 165 ) induced migration . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Most biological effects of VEGF are mediated via two receptor tyrosine kinases , VEGFR 1 and VEGFR 2 , but specific VEGF isoforms also bind neuropilins ( NP ) 1 and 2 , non tyrosine kinase receptors originally identified as receptors for semaphorins , polypeptides with essential roles in neuronal patterning . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
However , VEGFR 2 expression in the cytotrophoblastic and extravillous trophoblastic cells and VEGFR 1 , 3 and Ki 67 in the trophoblast populations were not significantly different between controls and cases ( P > 0 . 05 ) . ^^^ PlGF and soluble VEGFR 1 levels did not show any significance in study groups ( P > 0 . 05 ) . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
This was associated with decreased VEGF levels as well as VEGFR 2 but not VEGFR 1 expression . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Breast cancer cells express VEGF A , VEGF B , VEGF C and their receptors VEGFR 1 ( Flt 1 ) , VEGFR 2 ( Flk 1 / KDR ) and neuropilin ( NP 1 / NP 2 ) . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
VEGF A , the prototype VEGF ligand , binds and activates two tyrosine kinase receptors : VEGFR 1 ( Flt 1 ) and VEGFR 2 ( KDR / Flk 1 ) . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Vascular endothelial growth factor ( VEGF ) and its receptors VEGFR 1 , soluble ( s ) VEGFR 1 and VEGFR 2 represent a regulatory system , essential for both physiological and pathological angiogenesis . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Several compounds containing bulky lipophilic substituents at the benzamide pharmacophore yielded 10 to 17 fold selectivity for the VEGFR 2 versus VEGFR 1 kinase . . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
However , the mechanism of PlGF and VEGFR 1 mediated angiogenesis has remained unclear and some in vitro studies suggest that VEGF A / VEGFR 2 signaling may also play a role in PlGF mediated angiogenesis . ^^^ We also investigated the roles of VEGFR 1 and VEGFR 2 in PlGF 2 mediated angiogenesis . ^^^ Using a periadventitial collar technique , AdvPlGF 2 ( 1 10 10 ( 9 ) plaque forming units / ml ) was transferred to the adventitia of New Zealand White rabbits alone or together with adenoviruses encoding soluble VEGFR 1 ( sVEGFR 1 ) or soluble VEGFR 2 ( sVEGFR 2 ) . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
The present study sought to identify the sequence of events and the fate of endothelial cells , pericytes , and vascular basement membrane during capillary regression in mouse tracheas after VEGF signaling was blocked with a VEGF receptor tyrosine kinase inhibitor AG 013736 or soluble receptor construct ( VEGF Trap or soluble adenoviral VEGFR 1 ) . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
VEGF interacts with three subtypes of VEGF receptors occurring on the cellular membrane known as VEGFR 1 ( Flt 1 ) , VEGFR 2 ( Flk 1 / KDR ) , and VEGFR 3 ( Flt 4 ) . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Effects of VEGFR 1 , VEGFR 2 , and IGF IR hammerhead ribozymes on glucose mediated tight junction expression in cultured human retinal endothelial cells . ^^^ PURPOSE : To evaluate whether transfection of human retinal endothelial cells ( HRECs ) with plasmids expressing ribozymes designed to specifically cleave the mRNA and reduce expression of either vascular endothelial growth factor ( VEGF ) receptor 1 ( VEGFR 1 ) , or VEGF receptor 2 ( VEGFR 2 ) , or insulin like growth factor 1 receptor ( IGF IR ) modulates occludin expression in high glucose treated cells . ^^^ METHODS : Hammerhead ribozymes that specifically cleave the human VEGFR 1 , VEGFR 2 , and IGF IR mRNAs were developed and tested in vitro to determine ribozyme kinetics and cleavage specificity . ^^^ HRECs grown in normal ( 5 . 5 mM ) and high ( 25 mM ) glucose medium were transfected with plasmids expressing VEGFR 1 , VEGFR 2 , or IGF IR hammerhead ribozymes . ^^^ VEGF and IGF 1 levels were measured in conditioned medium of HREC exposed to high glucose conditions , and the effect of varying glucose concentration on VEGFR 1 and VEGFR 2 phosphorylation was examined . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
More important , PVF 1 binds to the human receptors VEGFR 1 ( Flt 1 ) and VEGFR 2 ( KDR ) and is able to induce angiogenesis in two model systems derived from vertebrates . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
This unorthodox antiangiogenic effect was mediated via VEGFR 1 activation and VEGFR 2 deactivation , the latter via Src homology domain 2 containing ( SH 2 containing ) tyrosine phosphatase 1 ( SHP 1 ) . ^^^ The VEGFR 1 specific ligand placental growth factor 1 ( PlGF 1 ) , but not VEGF E , which selectively binds VEGFR 2 , mimicked these responses . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
The most specific molecules displayed > 10 fold selectivity for VEGFR 2 over VEGFR 1 . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
VEGF activates VEGF receptor 1 ( VEGFR 1 ) and VEGFR 2 , whereas VEGF C activates VEGFR 2 and VEGFR 3 . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Resistin also significantly upregulated the mRNA expression of vascular endothelial growth factor receptors ( VEGFR 1 and VEGFR 2 ) and matrix metalloproteinases ( MMP 1 and MMP 2 ) at both mRNA and protein levels . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
OBJECTIVES : We have examined VEGF and its receptors , VEGFR 1 and VEGFR 2 , and angiopoietin 1 ( Ang 1 ) in the airways of subjects with asthma and contrasted these results with findings in normal control subjects . ^^^ MEASUREMENTS : We performed immunohistochemistry and image analysis to obtain quantitative measures of VEGF , VEGFR 1 , VEGFR 2 , and Ang 1 staining in airway biopsies , and ELISA to assess VEGF concentration in the bronchoalveolar lavage fluid . ^^^ VEGFR 1 was slightly higher in subjects with asthma and the VEGFR 1 : VEGFR 2 ratio was significantly higher in subjects with asthma . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Finally , we showed for the first time that Hsp 90 inhibitors also reduce expression of VEGFR 1 on human vascular endothelial cells , VEGFR 3 on lymphatic endothelial cells in vitro , and all three VEGFRs on mouse vasculature in vivo . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
VEGF receptor 1 , 2 , and 3 ( VEGFR 1 , VEGFR 2 , and VEGFR 3 , respectively ) expression increased throughout the time course , with maximal expression at 48 , 8 , and 72 h , respectively . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
VEGF , VEGFR 1 and VEGFR 2 immunoreactivity in the porcine arteries of vascular subovarian plexus ( VSP ) during the estrous cycle . ^^^ It binds to cell surface through ligand stimulatable tyrosine kinase receptors , the most important being VEGFR 1 ( flt 1 ) and VEGFR 2 ( flk 1 ) . ^^^ The aim of the present study was to immunolocalize VEGF and its two receptors : VEGFR 1 and VEGFR 2 in the broad ligament of the uterus in the area of vascular subovarian plexus during different phases of the estrous cycle in pig and to determine the correlation between immunoreactivity of the investigated factors and phases of the estrous cycle . ^^^ Specific polyclonal antibodies : anti VEGF , anti VEGFR 1 and anti VEGFR 2 were used . ^^^ All agents displayed phase related differences in immunoreactivity suggesting the modulatory effect of VEGF , VEGFR 1 and VEGFR 2 on the arteries of the VSP in the porcine broad ligament of the uterus . . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Here , we examined the expression of VEGF A and its receptors VEGFR 1 , VEGFR 2 , NP 1 , and NP 2 in conditionally immortalized mouse podocytes cultured in undifferentiated and differentiated conditions using RT PCR and Western analysis . ^^^ Differentiated podocytes expressed eightfold higher VEGFR 2 mRNA levels than undifferentiated podocytes , whereas VEGFR 1 , sVEGFR 1 , NP 1 , and NP 2 mRNA levels were similar . ^^^ VEGF ( 165 ) induced a twofold increase in VEGFR 2 mRNA and protein levels , whereas VEGFR 1 , sVEGFR 1 , NP 1 , and NP 2 mRNA levels remained unchanged . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
VEGF B is closely related to VEGF A and placenta growth factor ( PlGF ) , but unlike VEGF A , which binds to two receptor tyrosine kinases VEGFR 1 ( Flt 1 ) and VEGFR 2 ( Flk 1 / KDR ) , VEGF B and PlGF bind to VEGFR 1 and not VEGFR 2 . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
We induced apoptosis of microvascular EC using shear stress and the combined VEGF receptor ( VEGFR 1 and 2 ) inhibitor SU 5416 . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
VEGFR 1 immunoreactivity in the myometrial vascular smooth muscle cells was strongest in the controls . ^^^ Alterations in the placental bed expression of VEGFR 1 , VEGFR 2 , and Tie 1 , but not of VEGF and Tie 2 , may be associated with PE , IUGR , or DM . . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
To investigate the possible role of angiogenic mechanisms , we have studied the immunohistochemical expression of vascular endothelial growth factor ( VEGF ) , angiopoietin 1 ( Ang 1 ) , angiopoietin 2 ( Ang 2 ) and their receptors ( VEGFR 1 , VEGFR 2 , Tie 2 ) in ADPKD , Caroli ' s disease , normal and fetal livers . ^^^ Cholangiocytes were strongly positive for VEGF , VEGFR 1 , VEGFR 2 and Ang 2 in ADPKD and Caroli , and also for Ang 1 and Tie 2 in ADPKD , similar to fetal ductal plate cells . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
VEGFR 1 is required for the recruitment of haematopoietic precursors and migration of monocytes and macrophages , whereas VEGFR 2 and VEGFR 3 are essential for the functions of vascular endothelial and lymphendothelial cells , respectively . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Moreover , both these processes share similar modulating factors , like vascular endothelial growth factor ( VEGF ) and its receptors VEGFR 1 ( Flt 1 ) and VEGFR 2 ( Flk 1 ) . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
It blocked almost completely cell proliferation , potently reduced migration , assembly into tube like network on matrigel , and growth of capillaries into collagen gel . 15d PGJ ( 2 ) inhibited expression of VEGFR 1 and VEGFR 2 receptors both at mRNA and protein levels . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
VEGFA ( VEGF ) , VEGFB , VEGFC , VEGFD ( FIGF ) and PGF ( PlGF ) are VEGF family ligands for receptor tyrosine kinases , including VEGFR 1 ( FLT 1 ) , VEGFR 2 ( KDR ) and VEGFR 3 ( FLT 4 ) . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Vascular endothelial growth factor ( VEGF ) and VEGF receptor 1 ( VEGFR 1 / Flt 1 ) were shown to be involved in pathological angiogenesis , particularly rheumatoid arthritis ( RA ) . ^^^ Here we report that in a murine model of RA , deletion of the tyrosine kinase ( TK ) domain of VEGFR 1 decreased the incidence and clinical symptoms of RA . ^^^ Pathological symptoms , such as synovial hyperplasia , inflammatory infiltrates , pannus formation , and cartilage / bone destruction , became milder in Vegfr 1 tk ( / ) mice compared with wild type ( Wt ) mice in the human T cell leukemia virus 1 ( HTLV 1 ) pX induced chronic models . ^^^ VEGFR 1 TK deficient bone marrow cells showed a suppression of multilineage colony formation . ^^^ These results indicate that the VEGFR 1 TK signaling modulates the proliferation of bone marrow hematopoietic cells and immunity of monocytes / macrophages and promotes chronic inflammation , which may be a new target in the treatment of RA . . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
RTQ PCR demonstrated that VEGF , its receptors VEGFR 1 and VEGFR 2 , and angiopoietin 2 and its receptor ( Tie 2 ) were also overexpressed ( P < 0 . 05 ) in PTC . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Two high affinity receptors for vascular endothelial growth factor ( VEGF ) A , VEGFR 1 and VEGFR 2 , cooperate for physiological vasculogenesis and angiogenesis in embryogenesis . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Soluble VEGFR 1 and soluble VEGFR 2 modified the formation of coronary arteries , whereas soluble VEGFR 3 was without effect . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
There was a significant down regulation of VEGF and VEGFR 2 ( KDR ) but not VEGFR 1 ( Flt 1 ) gene expression by chrysin in LPS treated HUVEC cultures . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Both VEGFR 1 ( flt 1 ) and VEGFR 2 ( flk 1 ) are expressed on culture expanded human EPCs . ^^^ Previous studies suggest that the effect of VEGF on endothelial cell migration is primarily mediated via VEGFR 2 ; however , VEGF induced EPC migration in vitro was mediated by both receptors , suggesting that VEGF VEGFR 1 interactions in EPCs are distinct from differentiated endothelial cells . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
We simultaneously stained proliferation marker Ki 67 antigen and either VEGFR 1 ( Flt 1 ) or VEGFR 2 ( Flk 1 ) on paraffin embedded tissue sections from 50 cases of surgically resected human HCC . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Cellular GSH levels , GSH : GSSG ratios , VEGF A mRNA and protein expression , as well as VEGF A secretion , and VEGFR 1 and VEGFR 2 receptor expression were determined . ^^^ Further , thiol depletion by BSO caused a significant induction of VEGFR 1 and VEGFR 2 . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
CONCLUSIONS : These results indicated that neither the hyperpermeability in response to simultaneous stimulation of VEGFR 1 and VEGFR 2 nor VEGFR 1 mediated severe inflammation was associated with VEGF E ( NZ 7 ) / PlGF induced angiogenesis . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Retinal mRNA expression of VEGF splice variants ( VEGF 188 , VEGF 164 , VEGF 120 ) , VEGF receptors ( VEGFR 1 , VEGFR 2 , Npn 1 , Npn 2 ) , and pigment epithelium derived factor ( PEDF ) were also examined . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
In the present study , VEGF specific membrane receptor ( VEGFR 1 / Flt 1 and VEGFR 2 / Flk 1 ) expression was studied by confocal immunofluorescence in the corpus cavernosum of control rats , rats aged 12 and 18 months , and orchidectomized Wistar rats ( 90 days of bilateral orchidectomy ) . ^^^ However , in the aged rats , a shift resulting in a VEGFR 1 and VEGFR 2 co localization in the endothelial cell was observed . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
For example , levels of proangiogenic VEGF A , VEGF B , neuropilin 1 , VEGFR 1 , and VEGFR 2 were reduced and the levels of antiangiogenic thrombospondin 1 and retinoblastoma like 2 were increased . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
VEGFR 1 and 2 were detected in a few PBMCs and CD1a+ DCs . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
RESULTS : VEGF , VEGFR 1 and VEGFR 2 positive cells were immunodetected in E 14 and E 18 bladders . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
The salicylic amide 4a showed inhibition of both VEGFR 1 and VEGFR 2 tyrosine kinases at a 10 microM concentration . . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Vascular endothelial growth factor ( VEGF ) and its receptor tyrosine kinases , VEGFR 1 and VEGFR 2 , are important therapeutic targets for various cancers including AML . ^^^ We analyzed the feasibility of IHS to measure VEGFR 1 and VEGFR 2 expression in 28 AML samples using monoclonal antibodies ( moAbs ) against Flt 1 ( VEGFR 1 ) and KDR / Flk 1 ( VEGFR 2 ) . ^^^ Expression of VEGFR 1 ( + ) and VEGFR 2 ( + ) were seen in 25 % ( 7 / 28 ) and 43 % ( 12 / 28 ) respectively . ^^^ Forty six percent ( 13 / 28 ) were dual negatives for VEGFR 1 and VEGFR 2 ; 14 % ( 4 / 28 ) were dual positives for VEGFR 1 and VEGFR 2 . ^^^ An inferior survival was observed in patients whose myeloblasts express either VEGFR 1 ( + ) or VEGFR 2 ( + ) , or both . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Cell viability , VEGF and VEGF receptor ( VEGFR 1 and VEGFR 2 ) expression , and transcription factor activation were studied on transient exposure of monolayer EC to H2O2 . ^^^ Exposure to H2O2 increased VEGF and VEGFR 2 mRNA and protein in EC , without affecting VEGFR 1 expression . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
This effect resulted from the activation of VEGFR 2 , and was mimicked by vammin , a VEGFR 2 ligand from snake venom but not placenta growth factor , which binds specifically to VEGFR 1 . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
The analysis of mRNA expression of vascular endothelial growth factors ( VEGF ) and their receptors ( VEGFR 1 and VEGFR 2 ) showed a significant decrease in each VEGF isoform and in VEGFR 2 mRNA in representative alveolar wall tissues microdissected from the normal , NSIP , and UIP lungs . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
VEGF receptor 2 ( VEGFR 2 ) is critical for angiogenesis , although little is known about the precise role of endothelial VEGFR 1 and its downstream effectors in this process . ^^^ Here we have used a chimeric receptor approach in which the extracellular domain of the epidermal growth factor receptor was substituted for that of VEGFR 1 ( EGLT ) or VEGFR 2 ( EGDR ) and transduced into primary cultures of human umbilical vein endothelial cells ( HUVECs ) using a retroviral system . ^^^ Activation of eNOS by VEGFR 1 was dependent on Tyr 794 and was mediated via phosphatidylinositol 3 kinase , whereas VEGFR 2 Tyr 951 was involved in eNOS activation via phospholipase Cgamma 1 . ^^^ Consistent with these findings , the VEGFR 1 specific ligand placenta growth factor 1 activated phosphatidylinositol 3 kinase and VEGF E , which is selective for VEGFR 2 activated phospholipase Cgamma 1 . ^^^ Both VEGFR 1 and VEGFR 2 signal pathways converged on Akt , as dominant negative Akt inhibited the NO release and in vitro tube formation induced following activation of EGLT and EGDR . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
We examined the temporal and spatial expression of VEGF and its receptors , Flk 1 and Flt 1 , in the mouse uterus during the peri implantation period ( days 1 8 ) using Northern and in situ hybridization to assess the involvement of VEGF in the process of implantation . ^^^ The expression of the VEGF receptors , Flk 1 and Flt 1 , was also examined . ^^^ A single transcript ( approximately 6 . 5 7 . 0 kb ) for Flk 1 mRNA and two transcripts ( approximately 6 . 5 and 7 . 5 kb ) for that of Flt 1 were detected in poly ( A ) + uterine RNA samples . ^^^ On days 5 8 , cells in both the mesometrial and antimesometrial decidual beds exhibited accumulation of Flk 1 and Flt 1 mRNAs . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
The two genes for VEGF receptors , kinase insert domain containing receptor ( kdr ) and fms like tyrosine kinase 1 ( flt 1 ) , were found to be constitutively expressed in endothelial cells , and their relative mRNA levels were ranked in that order . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
We investigated the expression of vascular endothelial growth factor ( VEGF ) , an endothelial cell specific mitogen which is also known to induce vascular permeability in vivo , and its high affinity tyrosine kinase receptors flt 1 and KDR . ^^^ Endothelial cells did not express detectable amounts of VEGF mRNA but coexpressed flt 1 and KDR . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Two receptors for VEGF , KDR and flt 1 , were also demonstrated in most of the tumors examined . ^^^ KDR and flt 1 mRNA were limited to tumor endothelial cells and were more strongly expressed in the hepatic metastases than in the sinusoidal endothelium of the surrounding liver parenchyma . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
In addition , two VPF / VEGF receptors , flt 1 and kdr , are overexpressed by endothelial cells that line the microvessels that supply these tumors / inflammatory reactions . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
The vascular endothelial growth factor ( VEGF ) and its high affinity binding receptors , the tyrosine kinases Flt 1 and Flk 1 , are thought to be important for the development of embryonic vasculature . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Its effects are mediated by at least two distinct membrane spanning tyrosine kinase receptors , KDR and flt 1 ; the expression of which also seems restricted to vascular endothelium . ^^^ We describe here that cultured human retinal pigment epithelial ( HRPE ) cells express both KDR and flt 1 receptors , bind VAS / VEGF on two high affinity sites ( apparent Kd of 9 and 210 pM corresponding to 940 and 18 , 800 sites per cell ) and proliferate or migrate upon recombinant VAS / VEGF addition . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
We also examined the expression of the other VEGF receptor , flt 1 , during placental development . flk 1 , tek , and tie transcripts were detected sequentially at one half day intervals starting at E7 . 0 , suggesting that each of these RTKs play a unique role during vascularization of the mouse embryo . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
VEGF mRNA was also increased on day 3 , accompanied by an increase in flt family ( flt 1 and KDR / flk 1 ) mRNA expression . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Immunohistochemical analyses using antibodies against VEGF , bFGF , their receptors ( KDR , flt 1 , bek , and flg ) , factor 8 , and proliferating cell nuclear antigen were carried out on archival specimens of 52 human colon carcinomas and 10 adenomas . ^^^ The presence or absence of immunostaining for KDR , flt 1 , bek , and flg was evaluated in endothelial cells , and proliferation was determined by counting the number of proliferating cell nuclear antigen positive cells per 500 tumor cells . ^^^ Expression of VEGF and KDR was higher in metastatic than in nonmetastatic neoplasms and directly correlated with the extent of neovascularization and the degree of proliferation , whereas expression of bFGF , flt 1 , bek , and flg did not differ among tumor types . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
We used two transplantable rat glioma cells lines , C 6 and GS 9L , to analyze VEGF regulation in vitro and expression of VEGF and its high affinity tyrosine kinase receptors , flt 1 and flk 1 , in vivo . ^^^ In situ hybridization showed that VEGF is expressed in vivo in rat glioma cells which reside along necrotic areas and therefore closely mimicks the expression pattern of VEGF observed in human glioblastoma . flt 1 and flk 1 are specifically expressed in endothelial cells in the tumor and at the border between tumor and normal brain but are absent from endothelial cells in the normal brain proper . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
VEGF receptor subtypes KDR and FLT 1 show different sensitivities to heparin and placenta growth factor . ^^^ Vascular endothelial growth factor ( VEGF ) is an angiogenic growth factor which binds to two structurally related tyrosine kinase receptors denoted KDR and FLT 1 . ^^^ Bovine aortic endothelial ( ABAE ) cells and WM 35 melanoma cells were found to express KDR , while FLT 1 was primarily expressed on SK MEL 37 . ^^^ Heparin augmented VEGF binding to KDR expressing cells ( ABAE and WM 35 ) , but inhibited VEGF binding to FLT 1 expressing cells ( SK MEL 37 and WM 9 ) . ^^^ The concentration of heparin required for half maximal stimulation of VEGF binding to KDR expressing cells ( 500 ng / ml ) was 25 times greater than that required for half maximal inhibition of binding to FLT 1 expressing cells ( 20 ng / ml ) . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Moreover , the VPF / VEGF receptors , flt 1 and KDR , were up regulated in endothelial cells in superficial dermal microvessels . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
We also used in situ hybridization to study the expression of two recently described endothelial cell surface VPF receptors , flt 1 and kdr . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
The expression of VEGF , the closely related placental growth factor ( PIGF ) , the newly cloned endothelial high affinity VEGF receptors KDR and FLT 1 , and the endothelial orphan receptors FLT 4 and Tie were analyzed by in situ hybridization in normal human brain tissue and in the following CNS tumors : gliomas , grades 2 , 3 , 4 ; meningiomas , grades 1 and 2 ; and melanoma metastases to the cerebrum . ^^^ Significantly elevated levels of Tie , KDR , and FLT 1 mRNAs , but not FLT 4 mRNA , were observed in malignant tumor endothelia , as well as in endothelia of tissues directly adjacent to the tumor margin . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
KDR and flt 1 mRNA were coexpressed in endothelia of glomeruli and in peritubular capillaries in fetal and adult kidney . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
In contact reactions , mRNAs for two VPF / VEGF vascular endothelial cell receptors , flt 1 and KDR , were also strikingly overexpressed . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Different signal transduction properties of KDR and Flt 1 , two receptors for vascular endothelial growth factor . ^^^ Vascular endothelial growth factor ( VEGF ) is a homodimeric peptide growth factor which binds to two structurally related tyrosine kinase receptors denoted Flt 1 and KDR . ^^^ In order to compare the signal transduction via these two receptors , the human Flt 1 and KDR proteins were stably expressed in porcine aortic endothelial cells . ^^^ Binding analyses using 125I VEGF revealed Kd values of 16 pM for Flt 1 and 760 pM for KDR . ^^^ Cultured human umbilical vein endothelial ( HUVE ) cells were found to express two distinct populations of binding sites with affinities similar to those for Flt 1 and KDR , respectively . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
The FLT 4 , FLT 1 and KDR / FLK1 genes encode structurally similar endothelial cell receptor tyrosine kinases . ^^^ Recently it has been shown that the FLT 1 and KDR / FLK 1 proteins function as high affinity receptors for vascular endothelial growth factor ( VEGF ) . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Vascular endothelial growth factor ( VEGF ) is a potent angiogenic factor which binds to two structurally similar receptor tyrosine kinases , KDR and FLT 1 . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
In situ hybridization studies that were performed on joint tissues from patients with active RA revealed that synovial lining macrophages strongly expressed VPF / VEGF mRNA , and that microvascular endothelial cells of nearby blood vessels strongly expressed mRNA for the VPF / VEGF receptors , flt 1 and KDR . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Moreover , two VPF receptors , kdr and flt 1 , are overexpressed by papillary dermal microvascular endothelial cells . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Endothelial cells did not express detectable VPF mRNA , but did express high levels of mRNA for the VPF receptors flt 1 and KDR indicating that the endothelial cell staining likely reflects binding of VPF secreted by adjacent tumor cells . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
The related FLT 4 , FLT 1 , and KDR receptor tyrosine kinases show distinct expression patterns in human fetal endothelial cells . ^^^ The newly cloned FLT 4 receptor tyrosine kinase gene encodes a protein related to the VEGF receptors FLT 1 and KDR / FLK 1 . ^^^ A comparison of FLT 4 , FLT 1 and KDR / FLK 1 receptor mRNA signals shows overlapping , but distinct expression patterns in the tissues studied . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
VEGF and its receptors Flk 1 / KDR and Flt 1 may play a role in vascular development and regulation of vascular permeability . . ^^^ Flt 1 kinase has seven immunoglobulin like extracellular domains and a kinase insert sequence , features shared by two other human gene encoded proteins , kinase insert domain containing receptor ( KDR ) and FLT 4 . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Three receptor tyrosine kinases , FLT 1 , FLK 1 and FLT 4 , contain seven immunoglobin like domains in their extracellular region and are strongly related by sequence similarities to each other and , to a lesser degree , to the class 3 receptors CSF1R / FMS , PDGFR , SLFR / KIT and FLT3 / FLK2 . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
The endothelial cells of nearby stromal blood vessels also stained for VPF by immunohistochemistry and in addition expressed mRNAs encoding the VPF receptors flt 1 and kdr as determined by in situ hybridization . ^^^ Endothelial cells away from the tumor did not stain for VPF and no definite mRNA expression for flt 1 or kdr was detected by in situ hybridization . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Analysis of these cell lines for CD 34 , von Willebrand ' s factor , FLK 1 and FLT 1 expression , and capillary growth in Matrigel indicates that they appear to be endothelial cells , consistent with their original phenotype in vivo . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
VEGF / VPF expression by cells of hypoxic tissues coincides with expression of its two receptors , KDR and flt 1 , by ECs in the same tissues . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Vascular endothelial cell growth factor ( VEGF ) is a ligand for the tyrosine kinase receptor Flk 1 / KDR and Flt 1 and is considered to be an endothelial cell specific mitogen that plays an important role in angiogenesis . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
The endothelial cell specific vascular endothelial growth factor ( VEGF ) and its cellular receptors Flt 1 and Flk 1 have been implicated in the formation of the embryonic vasculature . ^^^ This is suggested by their colocalized expression during embryogenesis and the impaired vessel formation in Flk 1 and Flt 1 deficient embryos . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Several growth factor receptor tyrosine kinases have been implicated in angiogenesis , including receptors for epidermal , fibroblast , and platelet derived growth factors , as well as the receptors Flk 1 / KDR , Flt 1 Tek / Tie 2 , and Tie 1 . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Using Northern blot analysis , we further could show that human monocytes express only the gene for the VEGF receptor type , flt 1 , but not for the second known VEGF receptor , KDR . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Vascular endothelial growth factor and its receptors , flt 1 and flk 1 , are expressed in normal pancreatic islets and throughout islet cell tumorigenesis . ^^^ Two high affinity receptors for VEGF , flt 1 and flk 1 , are expressed by endothelial cells both in normal islets and in the stages of tumorigenesis ; these receptors are not up regulated during this process . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
To date , 3 structurally related cell surface receptors for VEGF , Flt 1 , Flt 4 and KDR , have been identified and shown to be human type 3 receptor tyrosine kinases . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
VEGF mediates endothelial cell proliferation , angiogenesis , vascular growth , and vascular permeability via the endothelial cell receptors , kinase insert domain containing receptor ( KDR ) / fetal liver kinase 1 ( Flk 1 ) and FLT 1 . ^^^ Arg 82 , Lys 84 and His 86 , located in a hairpin loop , were found to be critical for binding KDR / Flk 1 , while negatively charged residues , Asp 63 , Glu 64 , and Glu 67 , were associated with FLT 1 binding . ^^^ Identification of vascular endothelial growth factor determinants for binding KDR and FLT 1 receptors . ^^^ Mutations within the KDR site had minimal effect on FLT 1 binding , and mutants deficient in FLT 1 binding did not affect KDR binding . ^^^ Endothelial cell mitogenesis was abolished in mutants lacking KDR affinity ; however , FLT 1 deficient mutants induced normal proliferation . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Coexpression of flt 1 , flt 4 and KDR in freshly isolated and cultured human endothelial cells . ^^^ The class 3 receptor tyrosine kinases KDR and Flt 1 are high affinity receptors for VEGF , while Flt 4 is a receptor for the recently identified VEGF C . ^^^ We have examined the expression of flt 1 , flt 4 and KDR in human microvascular and large vessel endothelial cells and in a variety of other cell types in vitro . ^^^ Flt 1 , flt 4 and KDR were detected in both freshly isolated endothelial cells , and in sparse and confluent endothelial cell cultures by RT PCR . ^^^ Attempts to modulate receptor expression by culturing cells at reduced oxygen tensions ( 2 % ) did not induce consistent changes in flt 1 , flt 4 or KDR expression . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
The carboxyl terminal polypeptide ( 111 165 ) displayed no affinity for soluble kinase domain region ( KDR ) or Fms like tyrosine kinase ( FLT 1 ) receptors . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
VPF / VEGF stimulates endothelial cell growth and increases microvascular permeability by interacting with two endothelial cell tyrosine kinase receptors , KDR and flt 1 . ^^^ We studied 16 cases of AIDS associated Kaposi ' s sarcoma ( KS ) , 2 cases of cutaneous angiosarcoma , and 6 cases of capillary hemangioma by in situ hybridization for expression of VPF / VEGF , KDR , and flt 1 mRNAs . ^^^ Tumor cells in KS and angiosarcoma strongly expressed KDR but not flt 1 mRNA . ^^^ Endothelial cells in small stromal vessels in and around these tumors strongly expressed both KDR and flt 1 mRNAs . ^^^ The strong expression of both KDR and flt 1 in small stromal vessels in and around tumors suggests that VPF / VEGF may be an important regulator of the edema and angiogenesis seen in these tumors . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Two distinct receptors for vascular endothelial growth factor ( VEGF ) , the tyrosine kinase receptors Flt 1 and Flk 1 / KDR , have been described . ^^^ In contrast , endothelial cells expressing both the Flt 1 and the Flk 1 / KDR receptors produce more tissue factor upon stimulation with VEGF than after stimulation with PlGF . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
The purpose of the present study was to define the expression of VPF / VEGF and its receptors flt 1 and KDR in ovarian tumors . ^^^ In addition , microvascular endothelial cells strongly expressed mRNA of the VPF / VEGF receptors flt 1 and KDR and immunostained for VPF / VEGF protein in the majority of malignant and borderline tumors examined . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Histologic sections were subjected to in situ hybridization using 35S labeled riboprobes specific for VPF / VEGF and , in a subset of cases , riboprobes specific for the VPF / VEGF receptors flt 1 and KDR . ^^^ In addition , the endothelial cells of surrounding microvessels strongly expressed flt 1 and KDR mRNAs in all ten cases examined . ^^^ In contrast , no strong expression of VPF / VEGF , flt 1 , or KDR mRNA was observed in the seven examples of benign atrophic endometrium studied . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
The tyrosine kinases Flt 4 , Flt 1 , and Flk 1 ( or KDR ) constitute a family of endothelial cell specific receptors with seven immunoglobulin like domains and a split kinase domain . ^^^ Flt 1 and Flk 1 have been shown to play key roles in vascular development ; these two receptors bind and are activated by vascular endothelial growth factor ( VEGF ) . ^^^ VRP fails to bind appreciably to the extracellular domain of Flt 1 or Flk 1 . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
A number of growth factor receptor tyrosine kinases have been implicated in angiogenesis , including epidermal growth factor receptor , fibroblast growth factor receptor , platelet derived growth factor receptor , Flk 1 / KDR , Flt 1 , Tie 1 , and Tek / Tie 2 . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Northern analysis showed increased expression for both VEGF receptors ( flk 1 , flt 1 ) as well as the bFGF receptor 1 ( FGFR 1 ) in the collateral perfused region compared with that in the normally perfused region . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Identification of a natural soluble form of the vascular endothelial growth factor receptor , FLT 1 , and its heterodimerization with KDR . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
The presence of mRNA for aFGF , bFGF , and three isoforms of VEGF , as well as their receptors , FGFR 1 , FGFR 2 , Flt 1 , and KDR , in vessel outgrowths and the parent vessel , as identified by RT PCR , strongly implicated aFGF , bFGF , and VEGF as having an important role in this neovascularization response . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Vascular endothelial growth factor ( VEGF ) is an angiogenic inducer that mediates its effects through two high affinity receptor tyrosine kinases , Flt 1 and KDR . ^^^ Flt 1 is required for endothelial cell morphogenesis whereas KDR is involved primarily in mitogenesis . ^^^ Both Flt 1 and KDR have seven immunoglobulin ( Ig ) like domains in the extracellular domain . ^^^ Introduction of the second domain of KDR into an Flt 1 mutant lacking the homologous domain restored VEGF binding . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
VEGF , flk 1 , and flt 1 expression in a rat myocardial infarction model of angiogenesis . ^^^ Vascular endothelial growth factor ( VEGF ) is an endothelial cell mitogen that is thought to function by interacting with two high affinity receptors , flk 1 and flt 1 . ^^^ To determine the role played by VEGF , flk 1 , and flt 1 in this process in vivo , we studied the expression of the growth factor and its receptors in a rat infarct model . ^^^ After an acute myocardial infarction , we observed an initial rapid ( 1h ) rise in VEGF ( 275 % ) , flk 1 ( 375 % ) , and flt 1 ( 400 % ) mRNA expression throughout the entire heart . ^^^ Initial diffuse induction of VEGF , flk 1 , and flt 1 expression in the left ventricle was later replaced by an increase predominantly limited to perimyocardial infarction area where angiogenesis was taking place . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
AIDS KS cells and primary tumor tissues also expressed high levels of Flt 1 and KDR , the receptors for VEGF , while the normal skin of the same patients did not show any expression . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Uterine smooth muscle cells express functional receptors ( flt 1 and KDR ) for vascular permeability factor / vascular endothelial growth factor . ^^^ VPF / VEGF interacts with endothelial cells by way of two high affinity receptor tyrosine kinases : flt 1 and KDR . ^^^ In this study , we detected mRNA for flt 1 and KDR by in situ hybridization in smooth muscle cells in sections of the wall of the uterus . ^^^ Both uterine and colonic smooth muscle cells in culture expressed VPF / VEGF , but only smooth muscle cells from the uterus expressed mRNA for flt 1 and KDR by Northern analysis and in situ hybridization . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
To this end male Sprague Dawley rats were exposed to different hypoxic conditions such as 10 % or 8 % oxygen , 0 . 1 % carbon monoxide and cobalt chloride ( 60 mg / kg ) for 6 h and the abundance of flt 1 , flt 4 and flk 1 mRNA in lungs and livers was determined by RNase protection assay . ^^^ The relative proportions of flt 1 , flt 4 and flk 1 were 10 : 2 . 5 : 1 and 10 : 10 : 2 in normoxic lungs and livers , respectively . ^^^ It was found that 8 % but not 10 % oxygen increased flt 1 mRNA two to three fold in both organs , whilst flt 4 and flk 1 mRNA were not changed by acute inspiratory hypoxia . ^^^ Carbon monoxide inhalation also increased flt 1 mRNA but not flt 4 or flk 1 mRNA in both organs . ^^^ Subcutaneous cobalt administration increased flt 1 mRNA in the livers only , whilst flt 4 and flk 1 mRNA remained unchanged . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
VEGF possesses two high affinity receptors , KDR and FLT 1 . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Vascular Endothelial Growth Factor ( VEGF ) mediates its actions through the Flt 1 and KDR ( Flk 1 ) receptor tyrosine kinases . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Flt 1 , Flk 1 , and ACE containing cells were detected in 3 % O 2 treated explants , whereas 20 % O 2 explants were virtually negative . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Recombinant V 189 was purified from insect cells infected with a recombinant baculovirus as a nonmitogenic 50 kDa precursor that binds to the receptor Flt 1 but not to Flk 1 . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Rabbit uteri were examined using Northern and in situ hybridization to assess the temporal and spatial expression of VEGF and its receptor ( Flk 1 , Flt 1 ) mRNAs during the pre and peri implantation periods ( Days 0 8 ) . ^^^ VEGF receptor mRNAs were expressed in the uterus at all stages examined , with high levels of Flk 1 and Flt 1 at estrus and again just before implantation , 6 3 / 4 days pregnant . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
The specific receptors of VEGF , flt 1 and KDR were also detected in gastric mucosa . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Vascular endothelial growth factor ( VEGF ) stimulated the tyrosine phosphorylation of multiple components in confluent human umbilical vein endothelial cells ( HUVECs ) including bands of Mr 205 , 000 , corresponding to the VEGF receptors Flt 1 and KDR , and Mr 145 , 000 , 120 , 000 , 97 , 000 , and 65 , 000 70 , 000 . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Vascular endothelial growth factor ( VEGF ) receptor KDR ( kinase insert domain containing receptor ) is linked to endothelial cell proliferation , and VEGF receptor Flt 1 ( fms like tyrosine kinase ) is essential for the organization of embryonic vasculature . ^^^ RT PCR confirmed the presence of mRNA encoding the Flt 1 and KDR receptors as well as the endothelial form of cNOS in trophoblast cells . ^^^ Endogenous trophoblast DNA synthesis increased 3 fold in the presence of anti Flt 1 antibody but not in the presence of anti KDR antibody , suggesting that Flt 1 functions as a growth suppressive receptor to counteract the proliferative actions of KDR . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
A quantitative reverse transcription PCR study revealed that genes for VEGF receptors ( Flt 1 and KDR ) were expressed in HUVEC , but not in HOB , and that 1 , 25 ( OH ) 2D3 increased the levels of expression of VEGF receptor genes in endothelial cells only when cocultured with HOB . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
VEGF receptor tyrosine kinases , VEGF R 1 ( flt 1 ) and VEGF R 2 ( flk 1 ) , are induced in a tumour stage dependent manner during glioma progression and are exclusively expressed in tumour vascular endothelial cells . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
In order to explore factors regulating such angiogenesis and cyst formation in pilocytic astrocytoma , we examined expression of VEGF and its receptors ( KDR and Flt 1 ) using in situ hybridization . ^^^ KDR and Flt 1 were expressed in the tumor vasculature , with particularly high levels seen in coiled young proliferating vessels , especially those in the cyst wall . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Expression of vascular endothelial growth factor ( VEGF ) , also known as vascular permeability factor ( VPF ) , and its receptors Flt 1 and KDR ( Flk 1 in mouse ) and their localization in the human testis were analyzed by means of reverse transcriptase polymerase chain reaction ( RT PCR ) , Western blotting and immunohistochemistry . ^^^ Thus we demonstrate the presence of both types of VEGF receptors Flt 1 and KDR on Leydig as well as on Sertoli cells which are normal non endothelial cells , suggesting hitherto unrecognized and novel functions for such receptors . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
FLT 4 represents a recently cloned member of class 3 receptor tyrosine kinases which include receptors for the angiogenic growth factor VEGF , namely FLT 1 and KDR . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Vascular endothelial growth factor ( VEGF ) is an angiogenesis factor for which two signaling protein tyrosine kinase receptors , Flt 1 and KDR , have been identified . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Two receptors , KDR and Flt 1 , have been identified for VEGF . ^^^ In humans , Flt 1 appears to be the predominant receptor , whereas in the cow and sheep , KDR is the major receptor expressed . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
VEGF binds to two different tyrosine kinase receptors , kinase domain receptor ( KDR ) and Fms like tyrosine kinase 1 ( Flt 1 ) , and a number of VEGF homologs are known with distinct patterns of specificity for these same receptors . ^^^ Mapping the receptor binding determinants on a multiple sequence alignment of VEGF homologs , suggests the differences in specificity towards KDR and Flt 1 may derive from both sequence variation and changes in the flexibility of binding loops . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Characterization of the VEGF / PIGF receptors , KDR and flt 1 , revealed the presence of flt 1 mRNA in isolated cytotrophoblast and in vitro differentiated syncytiotrophoblast . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Surprisingly , potent and selective inhibitors of receptors involved in neovascularization such as FGFR , Flk 1 , or Flt 1 are less prevalent in the literature , and the discovery of TKIs that can inhibit angiogenesis remains a fertile area for drug discovery . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Two VEGF receptors , fms like tyrosine kinase 1 ( FLT 1 ) and KDR , have been identified almost specifically on human endothelial cells . ^^^ We could show that media conditioned by various cancer cell lines grown under hypoxic conditions were able to up regulate expression of FLT 1 mRNA and protein but not of KDR mRNA . ^^^ Both mutant VEGF , which activates only KDR , and placenta growth factor , which activates only FLT 1 , were able to enhance FLT 1 expression . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Interactions of FLT 1 and KDR with phospholipase C gamma : identification of the phosphotyrosine binding sites . ^^^ Vascular endothelial cell growth factor interacts with the receptor tyrosine kinases Flt 1 and KDR / Flk 1 . ^^^ We conclude that both Flt 1 and KDR have the potential to signal through PLC gamma via phosphotyrosine residues located in juxta membrane and carboxyl tail regions . . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
In the present study , expression of VEGF and its receptors , fetal liver kinase 1 ( flk 1 ) and fms like tyrosine kinase 1 ( flt 1 ) , was examined in rat carotid arteries after balloon injury . ^^^ Although VEGF and flk 1 were not detectable , high levels of flt 1 mRNA and protein were expressed by smooth muscle cells ( SMCs ) in the neointima , as demonstrated by en face in situ hybridization and Western blotting . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Vascular endothelial growth factor A ( here referred to as VEGF ) is an endothelium specific growth factor that binds to two distinct receptor tyrosine kinases , designated Flt 1 and KDR / Flk 1 . ^^^ In this study , we used porcine aortic endothelial ( PAE ) cells overexpressing KDR ( PAE / KDR ) to evaluate the interaction of KDR with intracellular proteins and compared them with Flt 1 expressing PAE cells ( PAE / Flt 1 ) . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
In addition , the expression of vascular endothelial growth factor ( VEGF ) and its receptors , KDR , Flt 1 and Flt 4 in oral SCCs was examined in relation to the vessel density and lymph node metastasis . ^^^ The expression of KDR and Flt 1 did not correlate with vessel density and lymph node metastasis . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Cells within the adherent population display characteristics of endothelial lineage including the expression of CD 31 , flk 1 , flt 1 , tie 2 , the capacity to take up acetylated LDL and the presence of cytoplasmic Weibel Palade bodies . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Recently , two endothelial cell surface receptors , flk 1 and flt 1 , have been shown to mediate the angiogenic activities of VEGF . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
VEGF binds to the related receptor tyrosine kinases Flt 1 and KDR / Flk 1 with high affinity , whereas PlGF binds only to Flt 1 . ^^^ Ligand stimulated KDR is known to transduce signals for cellular activity such as proliferation and migration , whereas weak or no responses have been recorded for Flt 1 . ^^^ We examined VEGF and PlGF for their capacity to stimulate signal transduction in porcine aortic endothelial cells expressing Flt 1 or KDR . ^^^ VEGF had essentially no effect on Flt 1 expressing cells , but induced DNA synthesis and migration of KDR expressing cells . ^^^ In agreement , MAP kinase , examined as a marker for DNA synthesis , was activated both by VEGF stimulation of the KDR cells and by PlGF stimulation of the Flt 1 cells . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
We studied the expression and function of VEGF and its receptors fms like tyrosine kinase ( Flt 1 ) and fetal liver kinase ( designated as kinase insert domain containing receptor , KDR in the human ) in the human epididymis . ^^^ Vascular endothelial cells were negative for Flt 1 but positive for KDR . ^^^ VEGF may act as a paracrine effector to influence the permeability of lymphatic vessels via Flt 1 , and of blood vessels via KDR . . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
The expression of Flk 1 , and flt 1 ( the two VEGF receptors ) and also of tie 2 , which is crucial for blood island formation , was detected as early as day 4 , and also on days 8 and 21 . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
VPF / VEGF induces vascular hyperpermeability , cell division , and other activities by interacting with two specific receptor tyrosine kinases , KDR / Flk 1 and Flt 1 , that are selectively expressed on vascular endothelium . ^^^ We used mouse mesentery , a tissue whose numerous microvessels are highly responsive to VPF / VEGF and that we found to express Flk 1 and Flt 1 selectively . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
The possible participation of VEGF and its high affinity tyrosine kinase receptors , flk 1 and flt 1 , in early background diabetic retinopathy was studied in the streptozotocin induced diabetic rat model of experimental retinopathy using in situ hybridization , blotting techniques , and immunohistochemistry . ^^^ Increased flk 1 and flt 1 mRNA levels were also found in the ganglion cell and both nuclear layers of diabetic samples only . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Vascular endothelial growth factor ( VEGF ) , a major regulator of angiogenesis , binds to two receptor tyrosine kinases , KDR / Flk 1 and Flt 1 . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
The expression of the vascular endothelial growth factor receptors flk 1 and flt 1 , as well as integrins and metalloproteinases ( which are involved in angiogenesis ) , were also affected . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Vascular endothelial growth factor ( VEGF ) binds to its receptor tyrosine kinase Flt 1 and KDR / Flk 1 and stimulates their autophosphorylation . ^^^ We examined the interactions of certain proteins with a SH 2 domain with Flt 1 and KDR using the yeast two hybrid system and found that Nck , SHP 2 , PLC gamma , and PI3K p 85 bind to Flt 1 . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
At least two tyrosine kinase receptors , Flk 1 and Flt 1 , mediate its action and are mostly expressed by endothelial cells . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Fetal and newborn explants had a highly complex network of branched vessels that immunoexpressed the flt 1 VEGF receptor , and flk 1 VEGF receptor expression was determined by reverse transcription PCR . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
In humans , the cellular actions of VEGF depend on binding to two specific receptors : Flt 1 and KDR . ^^^ The aims of this study were : ( 1 ) to localize VEGF receptor proteins in human renal ontogenesis ; ( 2 ) to quantify VEGF binding in human fetal and adult kidney ; and ( 3 ) to dissect the binding into its two known components : the KDR and Flt 1 receptors . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
The FLT 1 and KDR genes encode transmembrane tyrosine kinases which function as high affinity receptors for vascular endothelial growth factor ( VEGF ) . ^^^ We have used the baculovirus system to express the extracellular parts of the FLT 1 receptor and KDR receptor in soluble form ( sFLT 1 and sKDR ) , for in vitro binding and competition assays . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
The binding of VEGF to its two known receptors , KDR and Flt 1 , is modulated by cell surface associated heparin like glycosaminoglycans and exogenous heparin or heparan sulfate . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Flk 1 expression was inhibited dose dependently in EPA treated cells , whereas Flt 1 expression was increased in EPA treated cells . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
The VEGF receptors , kinase domain region ( KDR ) and Fms like tyrosine kinase ( Flt 1 ) , also were upregulated in the tumor vasculature of glioblastoma multiforme , anaplastic oligodendrogliomas , and ependymomas with necrosis , whereas the astrocytomas grade 2 , anaplastic astrocytomas , and oligodendroglioma tumors tended to express a weak to nondetectable signal . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Partial cDNAs encoding several RTKs were cloned from stellate cells activated in vivo , including those of Flt 1 , Flk 1 , c met , PDGFR , and Tyro10 / DDR2 . ^^^ RNAse protection from cells activated in vivo demonstrated biphasic induction of flt 1 and flk 1 mRNAs , receptors for vascular endothelial growth factor ( VEGF ) . ^^^ Culture activation of stellate cells was associated with increased [ 125I ] VEGF binding and Flt 1 and Flk 1 receptor protein . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
VEGF / PF R 1 ( flt 1 ) and VEGF / PF R 2 ( flk 1 ) are formed de novo in a glioma progression dependent manner . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
So far , two VEGF / VPF receptors ( VEGF / VPF R ) have been identified ( KDR , and flt 1 ) . ^^^ A third receptor ( flt 4 ) is highly correlated to KDR and flt 1 , but the true ligand for this receptor is still unknown . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
The aptamers potently inhibit the binding of VEGF to the human VEGF receptors , KDR and Flt 1 , expressed by transfected porcine aortic endothelial cells . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Receptor tyrosine kinases Flt 1 and Flk 1 / KDR , and their ligand , the vascular endothelial growth factor ( VEGF ) , were shown to be essential for angiogenesis in the mouse embryo by gene targeting . ^^^ Flt 1 differs from Flk 1 in that it displays a higher affinity for VEGF but lower kinase activity , suggesting the importance of its extracellular domain . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Vascular endothelial growth factor ( VEGF ) is a homodimeric glycoprotein that promotes angiogenesis and vascular hyperpermeability and interacts with two receptors , fms like tyrosine kinase ( Flt 1 ) and kinase domain containing region ( KDR ) . ^^^ Results of immunocytochemical and reverse transcription polymerase chain reaction analysis revealed that both protein and mRNA of VEGF , Flt 1 , and KDR were expressed by cultured normal EVT cells as well as their premalignant derivative produced by SV 40 Tag immortalization , and BeWo choriocarcinoma cells . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Expression of vascular endothelial growth factor ( VEGF ) and its receptors ( Flt 1 and Flk 1 ) following permanent and transient occlusion of the middle cerebral artery in the rat . ^^^ The present report describes the immunohistochemical distribution of VEGF and its 2 receptors , Flt 1 and Flk 1 at day 1 and 3 following permanent and transient middle cerebral artery occlusion ( MCAO ) in the rat . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
The effects of coronary artery disease ( CAD ) on human coronary microvascular responses to vascular endothelial growth factor ( VEGF ) and the alterations of the myocardial expressions of VEGF and its flk 1 and flt 1 receptors were examined in 48 patients . ^^^ CAD is associated with reduced vascular responses to both VEGF and HGF ; this is not likely due to a reduced expression of VEGF or flt 1 or flk 1 receptors and not due to a generalized endothelium dysfunction despite the presence of mild hypercholesterolemia in these patients with CAD . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
VEGF receptors 1 and 2 ( flt 1 and flk 1 ) as well as the recently identified angiopoietin receptors ( tie 1 and tie 2 ) are receptor tyrosine kinases specifically expressed in endothelial cells . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
To explore the signal transduction pathway for the effect of VEGF on SMCs , we studied the expression of 2 high affinity VEGF receptors , the kinase insert domain containing receptor ( KDR ) and flt 1 , in human SMCs . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Evidence for upregulation and redistribution of vascular endothelial growth factor ( VEGF ) receptors flt 1 and flk 1 in the oxygen injured rat retina . ^^^ In this study we probed for the two VEGF receptors ( VEGFRs ) known to have highest affinity in the rat flt 1 and flk 1 . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Interestingly , total tumor vascular mRNA expression of both Flk 1 and Flt 1 was greater in tumor vessels derived from the brain compared with tumor vessels derived from subcutaneous tissues . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Sck interacts with KDR and Flt 1 via its SH 2 domain . ^^^ It binds to its tyrosine kinase receptors , KDR and Flt 1 . ^^^ Sck also binds to Flt 1 and their binding is dependent on the kinase activities of KDR and Flt 1 . ^^^ As Sck contains the SH 2 domain and PTB domain , we tested whether they bind to KDR and Flt 1 . ^^^ On the other hand , the PTB domain of Sck does not bind to KDR and Flt 1 . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Receptor selective mutants with wild type KDR binding and decreased Flt 1 binding also induced KDR up regulation ; in contrast , mutants with decreased KDR binding and wild type Flt 1 binding did not , suggesting that KDR receptor signaling mediated the increase in KDR expression . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
They showed low levels of Flt 1 , Flk 1 , transforming growth factor ( TGF ) beta RI , and TGF beta RII expression but stained strongly with antibodies against Tie 1 and Tie 2 . ^^^ Brain , skin , and CCM EC lines did not significantly differ in their staining patterns with antibodies against Flt 1 , Flk 1 , TGF beta RI , TGF beta RII , Tie 1 , and Tie 2 . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
VEGF expression as well as the expression of 2 VEGF receptors , flt 1 and Flk 1 , were studied with immunohistochemical techniques in these samples at the different stages of human coronary atherosclerosis progression . ^^^ The immunostainings for flt 1 and Flk 1 were detected in aggregating macrophages in atherosclerotic lesions and also in endothelial cells of the microvessels in totally occlusive lesions . ^^^ CONCLUSIONS : These results demonstrate distinct expression of VEGF and its receptors ( flt 1 and Flk 1 ) in atherosclerotic lesions in human coronary arteries . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Archival specimens of 51 intestinal type and 38 diffuse type human gastric carcinomas were examined for tumor vessel counts , angiogenic factor expression , and the presence or absence of angiogenic factor receptors on tumor endothelium using antibodies against vascular endothelial growth factor ( VEGF ) and its receptors ( KDR and flt 1 ) , basic fibroblast growth factor ( bFGF ) and its receptors ( bek and flg ) , and factor 8 ( endothelial cells ) . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
To characterize molecular and cellular systems that may play a role in regulating blood vessel maturation , we have ( a ) analyzed the spatiotemporal expression of vascular endothelial growth factor ( VEGF ) and its receptors VEGF R 1 ( Flt 1 ) and VEGF R 2 ( Flk 1 ) throughout the ovarian cycle , ( b ) examined the recruitment of pericytes during vessel maturation , and ( c ) quantitatively measured the ratio of angiopoietin 2 ( Ang 2 ) to angiopoietin 1 ( Ang 1 ) throughout the ovarian cycle . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
This study aimed to investigate whether VEGF released from hypoxia exposed Hep G 2 cells alters expression of the two distinct receptors , kinase insert domain containing receptor ( KDR ) and fms like tyrosine kinase 1 ( flt 1 ) , in human umbilical venous endothelial cells ( HUVEC ) . ^^^ Hypoxia CM preferentially stimulated the mRNA expression of flt 1 but not that of KDR in HUVEC . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Peptides that inhibit binding of vascular endothelial growth factor ( VEGF ) to its receptors , KDR and Flt 1 , have been produced using phage display . ^^^ The peptides bind to a region of VEGF known to contain the contact surface for Flt 1 and the functional determinants for KDR binding . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Plasma cells in the bone marrow from patients diagnosed with multiple myeloma were found to express VEGF , whereas both the Flt 1 and KDR high affinity VEGF receptors were observed to be markedly elevated in the normal bone marrow myeloid and monocytic cells surrounding the tumor . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Expression patterns of the endothelial angiogenic receptors flt 1 , flk 1 , tie 1 , and tie 2 were similar to those described in human gliomas , but flt 1 was expressed also in neoplastic astrocytes , suggesting an autocrine role in tumor growth . ^^^ In crossbreeding experiments , hemizygous ablation of the tumor suppressor genes Rb and p 53 had no significant effect on the expression of VEGF , flt 1 , flk 1 , tie 1 , and tie 2 . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
The function of this endothelial cell selective growth factor is mediated by two homologous tyrosine kinase receptors , fms like tyrosine kinase 1 ( Flt 1 ) and kinase domain receptor ( KDR ) . ^^^ Although the functional consequence of vascular endothelial growth factor binding to the Flt 1 receptor is not fully understood , it is well established that mitogenic signaling is mediated by KDR . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
To investigate whether angiogenesis contributes to the pathogenesis of human colonic angiodysplasia , we examined the expression of basic fibroblast growth factor ( bFGF ) and vascular endothelial growth factor ( VEGF ) , and its endothelial cell receptors flt 1 and KDR . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
In all the hypervascular RCC tissues , the amounts of mRNAs for KDR and FLT 1 were increased compared to adjacent normal tissues . ^^^ The TIE 1 and FGFR 4 genes were also overexpressed in most of the hypervascular RCC tissues , while no mRNA of KDR , FLT 1 , or TIE 1 could be detected in any of the four human RCC cell lines . ^^^ These results suggest that KDR , FLT 1 , PlGF and TIE 1 mRNAs are present in the mesenchymal cells of RCC , while VEGF and FGFR 4 genes are expressed in RCC cells themselves in vivo . . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
The immunolocalization and gene expression of vascular endothelial growth factor ( VEGF ) and its cognate tyrosine kinase receptors , Flt 1 and KDR , has been studied in ocular melanomas and retinoblastomas using in situ hybridization and immunohistochemistry . ^^^ Flt 1 and KDR gene expression and immunolocalization occurred in VEGF expressing ganglion , Mller and amacrine cells in normal eyes . ^^^ Within the intra ocular tumours , VEGF receptor gene expression and protein was evident in the endothelial cells and also in cells close to the vessels , while in the adjacent retina , Flt 1 and KDR levels were elevated over normal , especially in the blood vessels . ^^^ Flt 1 and KDR were both observed at elevated levels in the choroid and iris blood vessels . ^^^ This study suggests that VEGF , Flt 1 and KDR are expressed by neural , glial and vascular elements within normal human retina . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
We obtained evidence that it is mainly vascular endothelial growth factor ( VEGF ) that mediates the angiogenic activity of insulin as follows . ( 1 ) Insulin upregulates the level of mRNA coding for secretory forms of VEGF , while the expression of the two VEGF receptor genes , kinase insert domain containing receptor ( kdr ) and fms like tyrosine kinase 1 ( flt 1 ) , was essentially unchanged by exposure to insulin . ( 2 ) A monoclonal antibody against human VEGF can completely neutralize both the proliferation and the tube formation of EC induced by insulin . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Expression of mRNA for vascular endothelial growth factor transmembraneous receptors Flt 1 and KDR , and the soluble receptor sflt in cycling human endometrium . ^^^ The aim of this study was to quantify and localize the mRNA expression of the vascular endothelial growth factor ( VEGF ) receptors Flt 1 , KDR and sflt , in human endometrium throughout the menstrual cycle . ^^^ While mRNA for the transmembraneous receptors Flt 1 and KDR was shown to be present at almost constant values throughout the menstrual cycle , the soluble receptor , sflt , had a three fold higher level of transcription during mid proliferative and late proliferative when compared with early proliferative and the entire secretory phase . ^^^ The expression of Flt 1 , KDR and sflt mRNA was detected in both isolated endometrial epithelial and stromal cell fractions . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
In an effort to obtain a more integrated understanding of vascular stroma formation in breast carcinoma , we examined expression of the angiogenic factor vascular permeability factor ( VPF ) / vascular endothelial growth factor ( VEGF ) ; the VPF / VEGF receptors flt 1 and KDR ; thrombospondin 1 , which has been reported to inhibit angiogenesis ; and the stromal components collagen type 1 , total fibronectin , ED A+ fibronectin , versican , and decorin by mRNA in situ hybridization on frozen sections of 113 blocks of breast tissue from 68 patients including 28 sections of breast tissue without malignancy , 18 with in situ carcinomas , 56 with invasive carcinomas , and 8 with metastatic carcinomas . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
RT PCR analysis showed the expression of two receptors for vascular endothelial growth factor ( flt 1 and KDR ) , and the endothelial nitric oxide synthase , adding further evidence of their identity as human ovarian microvascular endothelial cells ( HOMEC ) . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Production of VEGF and expression of the VEGF receptors Flt 1 and KDR in primary cultures of epithelial and stromal cells derived from breast tumours . ^^^ Production of vascular endothelial growth factor ( VEGF ) and expression of its receptors Flt 1 and KDR was determined in primary cultures of separated epithelial and stromal enriched cultures derived from ten primary human breast carcinomas . ^^^ Flt 1 and KDR receptors were analysed by semi quantitative reverse transcription polymerase chain reaction . ^^^ Thus , production of VEGF and expression of VEGF receptors Flt 1 and KDR by breast cancer epithelial and stromal cells suggests that VEGF may fulfil not only an angiogenic role , but also play a fundamental role as an autocrine / paracrine regulator in breast cancer , thereby facilitating tumour proliferation and subsequent invasion . . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Using an improved liposome enhanced labeling technique for the staining of weakly expressed antigens , subsets of this population could be identified which express the angiopoietin receptors TIE ( 67 . 6 % ) and TEK ( 36 . 8 % ) , the vascular endothelial growth factor receptors FLT 1 ( 7 % ) , FLT 4 ( 3 . 2 % ) , and KDR ( 10 . 4 % ) , or the receptor tyrosine kinases HER 2 ( 15 . 4 % ) and FLT 3 ( CD 135 ; 77 . 6 % ) . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Bioactivity of anti angiogenic ribozymes targeting Flt 1 and KDR mRNA . ^^^ Vascular endothelial growth factor ( VEGF ) and its receptors Flt 1 and KDR play important roles in physiological and pathological angiogenesis . ^^^ Ribozymes targeting Flt 1 or KDR mRNA sites reduced VEGF induced proliferation of cultured human vascular endothelial cells and specifically lowered the level of Flt 1 or KDR mRNA present in the cells . ^^^ Anti Flt 1 and KDR ribozymes also exhibited anti angiogenic activity in a rat corneal pocket assay of VEGF induced angiogenesis . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Expression of vascular endothelial growth factor / vascular permeability factor ( VEGF / VPF ) and its receptors ( flt 1 and flk 1 ) during the peri implantation period ( days 3 , 4 , 5 , 6 and 7 post coitus ) in the golden hamster was investigated by in situ hybridization , immunohistochemistry and the reverse transcription / polymerase chain reaction ( RT PCR ) . ^^^ Flt 1 but no flk 1 immunoreactivity was observed . ^^^ At day 4 , the subepithelial stroma and embryo displayed immunoreactivity for VEGF and flt 1 , whereas endothelial cells expressed both flt 1 and flk 1 . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Expression of vascular endothelial growth factor ( VEGF ) and its corresponding receptors ( flt 1 and flk 1 ) in the bovine oviduct . ^^^ Reverse transcription polymerase chain reaction ( RT PCR ) and ribonuclease protection assay ( RPA ) were used to show that the bovine oviduct expresses VEGF and its two receptors flk 1 and flt 1 . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Alterations in the immunohistochemical distribution patterns of vascular endothelial growth factor receptors Flk 1 and Flt 1 in bleomycin induced rat lung fibrosis . ^^^ To investigate the role of vascular endothelial growth factor ( VEGF ) in fibrogenesis , the distribution patterns of the VEGF receptors Flt 1 and Flk 1 were studied by immunohistochemistry , double immunofluorescence , and immunoelectron microscopy in normal ( n=2 ) and bleomycin treated ( n=21 ) adult rats . ^^^ Flt 1 , Flk 1 , and VEGF immunoreactivity localised predominantly to the pulmonary epithelium . ^^^ In control lungs , Flt 1 immunoreactivity was present in ciliated bronchial epithelium and type 2 pneumocytes , Flk 1 in Clara cells , and VEGF in Clara cells and type 2 pneumocytes . ^^^ Flt 1 and Flk 1 mRNAs were observed in Clara cells and type 2 pneumocytes . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
The expression of VEGF and its two receptors , Flk 1 and Flt 1 , is pivotal for the proper formation of blood vessels in embryogenesis as shown by gene targeting experiments . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Immunolocalisation of the VEGF receptors FLT 1 , KDR , and FLT 4 in diabetic retinopathy . ^^^ AIM : To determine the spatial and temporal changes in the staining pattern of the VEGF receptors FLT 1 , KDR , and the putative receptor FLT 4 during the pathogenesis of diabetic retinopathy . ^^^ METHODS : Immunohistochemical localisation of VEGF receptors , using antibodies against FLT 1 , FLT 4 , and KDR , was carried out on specimens of normal human retina ( n = 10 ) , diabetic retinas ( a ) with no overt retinopathy ( n = 12 ) , ( b ) with intraretinal vascular abnormalities but no proliferative retinopathy ( n = 5 ) , ( c ) with active proliferative retinopathy ( n = 6 ) , and ( d ) with no residual proliferative retinopathy after scatter photocoagulation therapy ( n = 14 ) , and surgically excised diabetic fibrovascular membranes ( n = 11 ) . ^^^ In contrast with FLT 1 staining minimal immunostaining for KDR was demonstrated in the non diabetic eyes and the unlasered eyes ; however , weak staining for KDR was observed in the inner nuclear layer and the ganglion cell layer of the unlasered eyes with diabetic changes . ^^^ CONCLUSIONS : This study supports a role for FLT 1 , KDR , and possibly FLT 4 in the pathogenesis of diabetic retinopathy ; however , their specific roles in the progression of the disease may differ . . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
OBJECTIVE : To determine the concentration of vascular endothelial growth factor in the seminal fluid , the presence of vascular endothelial growth factor receptors Flt 1 and KDR in spermatozoa , and the predictive value of seminal vascular endothelial growth factor on fertilization and the chance of pregnancy in patients undergoing intracytoplasmic sperm injection ( ICSI ) or IVF . ^^^ Expression of vascular endothelial growth factor receptors ( Flt 1 , KDR ) on spermatozoa was demonstrated . ^^^ Vascular endothelial growth factor receptors Flt 1 and KDR were detected on spermatozoa for the first time . . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Quantitative reverse transcription polymerase chain reaction analysis reveals a time and dose dependent up regulation of mRNA for VEGF receptors ( KDR and flt 1 ) . ^^^ The up regulation of KDR and flt 1 mRNA is also mimicked by the thrombin receptor activating peptide . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
In addition , all cell lines and primary tumours expressed the mitogenic VEGF receptor FLK 1 , whilst the non mitogenic receptor FLT 1 was less frequently positive , suggesting that the tyrosine kinase FLK 1 is involved in malignant transformation of neuroblastoma cells . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
The mRNA levels for both types of VEGF receptors , Flk 1 and Flt 1 were low but detectable in FRC cells by RT PCR and were not changed by OP 1 . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
These cells express transcripts of the two VEGF receptors KDR and Flt 1 . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
VEGF binds to two cell surface tyrosine kinase receptors , KDR ( kinase domain region ) and Flt 1 ( fms like tyrosine kinase 1 ) . ^^^ Overall , KDR was seen in 59 % of glomeruli , Flt 1 in 45 % and sFlt in 57 % . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
The HEEC were very responsive to VEGF growth stimulation likely due to elevated affinity , or increased levels of , KDR and FLT 1 on the cell surface . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
VEGF exerts its angiogenic and pro tumorigenic properties by way of two high affinity receptors , fms like tyrosine kinase 1 ( FLT 1 ) and fetal liver kinase 1 ( FLK 1 ) . ^^^ Immunohistochemical analysis using antihuman FLT 1 and FLK 1 was performed and specimens were analyzed to characterize the expression and distribution of both receptors . ^^^ Immunocytochemical analysis for FLT 1 and FLK 1 was also performed on cultured PCa cell lines ( DU 145 and LNCaP ) . ^^^ Vascular endothelial cells ( VECs ) within areas of PCa consistently expressed both FLT 1 and FLK 1 receptors . ^^^ FLT 1 and FLK 1 were both expressed in BPH tissue . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
To investigate the role of VEGF in maintaining the absorptive function of the intramembranous microvessels , the present study was undertaken to determine the gestational change in gene expression of VEGF and its receptors , kinase insert domain containing receptor ( KDR ) and fms like tyrosine kinase ( Flt 1 ) , in ovine placenta , chorion , and amnion . ^^^ The relative abundance of VEGF , KDR , and Flt 1 mRNA was determined by Northern blot analysis , and VEGF molecular forms expressed were identified by reverse transcriptase polymerase chain reaction . ^^^ In the placenta , KDR was the primary VEGF receptor expressed , although Flt 1 was also detected at very low levels . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Significant positive correlations were found between VEGF C and flt 4 , VEGF and KDR , VEGF and flt 1 in tumour tissues . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Vascular endothelial growth factor ( VEGF ) is an angiogenic growth factor that acts via two high affinity receptors ( KDR and Flt 1 ) , and its production is increased in preeclampsia . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
The expression of vascular endothelial growth factor ( VEGF ) and its receptors Flt 1 and Flk 1 in the rat kidney was examined during ontogeny using Northern blot analysis and immunocytochemistry . ^^^ In prevascular embryonic kidneys ( embryonic day 14 [ E 14 ] ) , immunoreactive Flt 1 and Flk 1 were observed in isolated angioblasts , whereas VEGF was not detected . ^^^ In late fetal kidneys ( E 19 ) , immunoreactive VEGF was detected in glomerular epithelial and tubular cells , whereas Flt 1 and Flk 1 were expressed in contiguous endothelial cells . ^^^ VEGF induced upregulation of Flk 1 and Flt 1 expression , as assessed by Western blot analysis . ^^^ Flt 1 , Flk 1 , and angiotensin converting enzyme containing cells were detected in VEGF treated explants , whereas control explants were negative . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
After binding to its specific receptors KDR and FLT 1 on the endothelial cell surface cell proliferation and migration are stimulated . ^^^ We investigated the protein and mRNA expression of KDR and FLT 1 in native tissues and tumor cell cultures from squamous cell carcinomas of the head and neck ( HNSCC ) and analyzed their in vitro functional significance for tumor cell proliferation and migration . ^^^ Apart from the expected expression of VEGF receptors on endothelial cells we observed a tumor cell specific localization of FLT 1 in 29 tumors and KDR in 16 of 37 tumors analyzed . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
VEGF is expressed in glioma cells and its receptors ( Flt 1 and KDR ) are expressed in the same gliomas . ^^^ KDR is a receptor for the various VEGF isoforms and for VEGF C ; Flt 1 is a receptor for the various isoforms . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Consequently , we sought to evaluate by antisense knockdown of gene expression the contribution of VEGF receptors ( Flt 1 and Flk 1 ) on these events . ^^^ The capacity to inhibit the protein synthesis of Flt 1 and Flk 1 by antisense oligonucleotides provides a new approach to block VEGF pathological effects in vivo . . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
We examined VEGF mRNA expression , VEGF isoform pattern and VEGF receptor ( flt 1 and KDR ) by RT PCR analysis in 30 osteosarcomas . ^^^ All 30 osteosarcomas expressed VEGF mRNA . 17 osteosarcomas ( 57 % ) expressed flt 1 mRNA , whilst 20 ( 67 % ) expressed KDR mRNA . 6 / 30 ( 20 % ) osteosarcomas were positive for VEGF 121 only , 8 ( 27 % ) for VEGF 121 + VEGF 165 , and 16 ( 53 % ) for VEGF 121 + VEGF 165 + VEGF 189 . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Melanoma associated expression of vascular endothelial growth factor and its receptors FLT 1 and KDR . ^^^ The expression patterns of vascular endothelial growth factor ( VEGF ) and its two receptors , flt 1 and KDR , were assessed in normal human melanocytes , transformed melanocytes expressing the simian virus 40 Tgene ( SV40T ) , and melanoma cells derived from primary and metastatic lesions . ^^^ Constitutive expression of VEGF , flt 1 , and KDR mRNA and proteins was observed in the majority of primary and metastatic melanoma cell lines , and in SV40T transformed melanocytes . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Endothelial cell tyrosine kinase receptors , KDR and Flt 1 , have been implicated in VEGF responses including cellular migration , proliferation , and modulation of vascular permeability . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
A KDR receptor selective mutant increased eNOS expression , whereas an Flt 1 receptor selective mutant did not . ^^^ Furthermore , VEGF treatment increased eNOS expression in a KDR but not in an Flt 1 receptor transfected porcine aorta endothelial cell line . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Vascular endothelial growth factor receptors 1 3 , also known as flt 1 , KDR , and flt 4 , are endothelial cell specific receptor tyrosine kinases which serve as key mediators of the angiogenic responses . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Reverse transcription polymerase chain reaction analysis demonstrated the presence of VEGF receptor mRNA ( flt 1 and KDR ) in HMCs . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Using two independent experimental strategies , we were able to prove that VEGF receptor KDR , but not VEGF receptor Flt 1 , can induce NO release in endothelial cells . ^^^ First , we made use of porcine aortic endothelial cells ( PAE ) expressing either KDR or Flt 1 . ^^^ While KDR expressing PAE / KDR cells responded to VEGF A stimulation with a significant elevation of intracellular cGMP already after 2 min , Flt 1 expressing PAE / Flt 1 cells did not show any signal in this RIA based cGMP assay . ^^^ In a second experimental strategy freshly isolated human umbilical vein endothelial cells ( HUVEC ) were stimulated either with the KDR specific ligand VEGF E or with the Flt 1 specific ligand PIGF 2 . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Using immunohistochemistry , the results demonstrated that the tissue recombinants typically exhibited an overexpression of EGF , EGF R , bFGF , TGF beta ( 1 ) together with a concurrent downregulation of TGF alpha , IGF 1 , IGF 2 , and VEGF receptors ( flk 1 , flt 1 ) . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Co expression of vascular endothelial growth factor ( VEGF ) and its receptors ( flk 1 and flt 1 ) in hormone induced mammary cancer in the Noble rat . ^^^ The action of VEGF is mediated by two high affinity receptors with ligand stimulated tyrosine kinase activity : VEGFR 1 / flt 1 and VEGFR 2 / flk 1 , which are expressed mainly in vascular endothelial cells . ^^^ Intense reactivities to VEGF , flt 1 and flk 1 were observed in mammary cancer cells , especially in invasive mammary carcinoma . ^^^ Western blot analysis confirmed the increase in flk 1 and flt 1 proteins in induced mammary cancers . ^^^ To further verify this hypothesis , we investigated the correlation between cellular proliferation and the expression of VEGF , flt 1 and flk 1 . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
There was differential expression of the VEGF receptors , Flt 1 and Flk 1 , in hypoxic T 6 cells . ^^^ Hypoxic conditions selectively stimulated Flt 1 mRNA expression , whereas Flk 1 mRNA remained unchanged . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
We conducted the present study to determine , by immunocytochemistry and computerized image analysis of the functionalis , the expression and modulation of the receptors Flk 1 / KDR and Flt 1 , which mediate VEGF effects on endothelial mitogenicity , chemotaxis , and capillary permeability . ^^^ The number of capillaries immunostained for Flk 1 / KDR was maximal in the proliferative phase ( ratio Flk 1 / CD34 : 1 ) , twice as high as the number of Flt 1 expressing capillaries ( ratio Flt 1 / CD34 : 0 . 47 ) . ^^^ Enhanced expression of Flk 1 / KDR , and of Flt 1 , on narrow capillary strands at the beginning of and during the proliferative phase may account for the rapid capillary growth associated with endometrial regeneration following menstrual shedding . ^^^ The high coexpression of Flk 1 / KDR and Flt 1 observed on capillaries during the midsecretory period correlates with an increase of endometrial microvascular density and of permeability characteristic of this phase of the cycle , which is a prerequisite for implantation . ^^^ Finally , strong expression of Flt 1 , but not Flk 1 / KDR , was observed on dilated capillaries during the premenstrual period and the late proliferative phase , suggesting preferential association of Flt 1 with nonproliferating capillaries at those times ; activation of this receptor by VEGF could be involved in premenstrual vascular hyperpermeability , edema , and extravasation of leukocytes . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
This study was initiated to identify signaling proteins used by the receptors for vascular endothelial cell growth factor KDR / Flk1 , and Flt 1 . ^^^ Neither placental growth factor , which activates Flt 1 , epidermal growth factor ( EGF ) , or fibroblast growth factor ( FGF ) induced tyrosine phosphorylation of PLCgamma , indicating that KDR is uniquely important to PLCgamma activation in HUVEC . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
ZD 4190 , a substituted 4 anilinoquinazoline , is a potent inhibitor of KDR and Flt 1 RTK activity , and VEGF stimulated HUVEC proliferation in vitro . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Vegf , Vegf B , Vegf C and their receptors KDR , FLT 1 and FLT 4 during the neoplastic progression of human colonic mucosa . ^^^ Because the crucial role of angiogenesis has been demonstrated in tumor growth and metastasis , the present study was undertaken to characterize the relative expression of vascular endothelial growth factors VEGF ( vascular endothelial growth factor ) , VEGF B , VEGF C , and their receptors KDR ( kinase insert domain containing receptor ) , FLT 1 ( fms like tyrosine kinase ) , and FLT 4 in human colonic cancers , in relation to the Astler Coller pathological classification , and to prognosis . ^^^ In contrast to KDR and FLT 4 , the expression of VEGF C and FLT 1 genes increased in some pathological tissues . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Homeostatic modulation of cell surface KDR and Flt 1 expression and expression of the vascular endothelial cell growth factor ( VEGF ) receptor mRNAs by VEGF . ^^^ Regulation of the receptor tyrosine kinases , KDR and Flt 1 , to which VEGF binds on endothelial cells is incompletely understood . ^^^ Chronic incubation with tumor conditioned medium or VEGF diminished ( 125 ) 1 VEGF binding to human umbilical vein endothelial cells , incorporation of ( 125 ) 1 VEGF into covalent complexes with KDR and Flt 1 , and immunoreactive KDR in cell lysates . ^^^ Preincubation with VEGF or tumor conditioned medium down regulated cell surface receptor expression but up regulated KDR and Flt 1 mRNAs , an effect abrogated by a neutralizing VEGF antibody . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Despite much progress in recent years , the precise signalling events triggered by the vascular endothelial growth factor ( VEGF ) receptors , fms like tyrosine kinase ( Flt 1 ) and kinase insert domain containing receptor ( KDR ) , are incompletely defined . ^^^ Results obtained when Flt 1 and KDR are individually expressed in fibroblasts or porcine aortic endothelial cells have not been entirely consistent with those observed in other endothelial cells expressing both receptors endogenously . ^^^ It has also been difficult to demonstrate VEGF induced phosphorylation of Flt 1 , which has led to speculation that KDR may be the more important receptor for the mitogenic action of VEGF on endothelial cells . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
The different VEGF ' s recognize signalling tyrosine kinase receptors ( Flt 1 , Flk 1 and Flt 4 ) and accessory receptors . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
VEGF functions via binding to the VEGF receptors Flk 1 and Flt 1 . ^^^ We hypothesized that this might occur via reduced VEGF , TGF beta ( 1 ) , bFGF , Flk 1 , and Flt 1 gene expression at rest and after exercise . ^^^ VEGF , TGF beta ( 1 ) , bFGF , Flk 1 , and Flt 1 mRNA were analyzed from the left gastrocnemius by quantitative Northern blot . ^^^ Exercise increased VEGF mRNA 4 . 8 fold , TGF beta ( 1 ) mRNA 1 . 6 fold , and Flt 1 mRNA 1 . 7 fold but did not alter bFGF or Flk 1 mRNA measured 1 h after exercise . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
VEGF A stimulates many actions of endothelial cells including proliferation , migration , and nitric oxide release via binding to and activation of the two primarily endothelial specific receptor tyrosine kinases KDR and Flt 1 . ^^^ KDR and Flt 1 stimulate multiple signal transduction pathways in endothelial cells . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Roles of two VEGF receptors , Flt 1 and KDR , in the signal transduction of VEGF effects in human vascular endothelial cells . ^^^ VEGF expresses its effects by binding to two VEGF receptors , Flt 1 and KDR . ^^^ However , properties of Flt 1 and KDR in the signal transduction of VEGF mediated effects in endothelial cells ( ECs ) were not entirely clarified . ^^^ We investigated this issue by using two newly developed blocking monoclonal antibodies ( mAbs ) against Flt 1 and KDR . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Biologically active VEGF was expressed along a time course that paralleled the expression of two specific VEGF receptors , Flk 1 and Flt 1 , and the progression of joint disease . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Two VEGF tyrosine kinase receptors have been reported fms like tyrosine kinase 1 ( Flt 1 ) and kinase domain region ( KDR ) . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
OBJECTIVE : The aim of this study has been to evaluate the clinical significance of expression of VEGF and its receptors , Flt 1 , KDR , and Flt 4 , in endometrial carcinomas . ^^^ The overall positive rates in the 86 carcinoma specimens were 66 % for VEGF , 51 % for Flt 1 , 38 % for KDR , and 57 % for Flt 4 . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Flt 1 ( VEGF receptor 1 ) and KDR / Flk 1 ( VEGF receptor 2 ) are the high affinity receptors for the angiogenesis factor , vascular endothelial growth factor ( VEGF ) . ^^^ We immunohistochemically examined expressions and localizations of Flt 1 and KDR in 28 surgically resected HCC tissues . ^^^ In non cancerous area , Flt 1 and KDR were mainly found in macrophages including Kupffer cells ; both receptors were found in vascular endothelial cells in the portal veins and arteries within portal tracts ; and KDR was also found in some sinusoidal endothelial cells . ^^^ In cancerous area , Flt 1 and KDR were found in some macrophages , and also in the endothelial cells of intratumoral blood vessels . ^^^ These results suggest that there would be an angiogenesis mechanism via VEGF / Flt 1 or VEGF / KDR in HCC , and the VEGF / KDR system would take a more important role . . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Vascular endothelial growth factor ( VEGF ) , a potent mitogen , is expressed in podocytes in the glomerulus , and VEGF receptors ( flt 1 , KDR , and neuropilin 1 ) are present on endothelial cells and other cell types . ^^^ No mesangial flt 1 receptor staining was seen in normal renal cortical tissue samples , and only weak mesangial KDR staining was detected . ^^^ In contrast , mesangial flt 1 and KDR receptor staining were both clearly seen in biopsy samples from proliferative renal diseases . ^^^ In conclusion , flt 1 , KDR , and neuropilin 1 are present on cultured HMC , and VEGF ( 165 ) induces HMC proliferation . ^^^ In addition , the flt 1 and KDR receptors are expressed in the mesangium in mesangioproliferative disease . . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Properties of two VEGF receptors , Flt 1 and KDR , in signal transduction . ^^^ The properties of two VEGF receptors , Flt 1 and KDR , in the signal transduction of VEGF in human umbilical vein endothelial cells ( HUVECs ) were investigated by using two newly developed blocking monoclonal antibodies ( mAbs ) against Flt 1 and KDR . ^^^ The KDR / Flt 1 heterodimer and the KDR homodimer mediate the expression of Ets 1 , MMP 1 , and Flt 1 . ^^^ The KDR / Flt 1 heterodimer and the KDR homodimer are required for the assembly of vinculin in focal adhesion plaque by regulating the phosphorylation of focal adhesion kinase ( FAK ) and paxillin . . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Vascular endothelial growth factor ( VEGF ) is a pleiotropic factor that exerts a multitude of biological effects through its interaction with two receptor tyrosine kinases , fms like tyrosine kinase ( Flt 1 ) or VEGF receptor 1 and kinase insert domain containing receptor ( KDR ) or VEGF receptor 2 . ^^^ This variant bound with wild type affinity to Flt 1 , was at least 470 fold reduced in binding to KDR , and had no activity in cell based assays measuring autophosphorylation of KDR or proliferation of primary human vascular endothelial cells . ^^^ Both variants had approximately wild type affinity for KDR , were about 2000 fold reduced in binding to Flt 1 , and showed activity comparable with the wild type protein in KDR autophosphorylation and endothelial cell proliferation assays . ^^^ These variants will serve as useful reagents in elucidating the roles of Flt 1 and KDR . . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
VEGF receptors , the tyrosine kinases Flt 1 and KDR , are expressed on vascular endothelial cells and initiate angiogenesis upon activation by VEGF . 1 Anilino ( 4 pyridylmethyl ) phthalazines , such as CGP 79787D ( or PTK 787 / ZK 222584 ) , reversibly inhibit Flt 1 and KDR with IC ( 50 ) values < 0 . 1 microM . ^^^ Modification of the 1 anilino moiety afforded derivatives with higher selectivity for the VEGF receptor tyrosine kinases Flt 1 and KDR compared to the related receptor tyrosine kinases PDGF R and c Kit . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
To test this hypothesis , we evaluated the expression and integrity of vascular endothelial growth factor , a potent angiogenic mediator , and its receptors , Flt 1 and KDR , in chronic venous leg ulcerations . ^^^ Flt 1 and KDR protein and mRNA were detected in the papillary vessels in close vicinity to the lesional epithelium of chronic wounds . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
In the present study , the expression of vascular endothelial growth factor ( VEGF ) , an endothelial cell specific angiogenic mitogen , and its receptors , fms like tyrosine kinase ( Flt 1 ) and kinase insert domain containing receptor ( KDR ) , in the lungs of five cases with PH , were examined . ^^^ The expression of Flt 1 mRNA was observed in endothelial cells of the arteries adjacent to the plexiform lesions , while KDR mRNA was expressed in the endothelial cells inside the plexiform lesions . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
However , vascular endothelial growth factor specific receptors flt 1 and KDR mRNA expression are absent in normal sinusoid vessels surrounding vascular endothelial growth factor producing secondary hepatic tumors . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Expression of vascular endothelial growth factor isoforms and their receptors Flt 1 , KDR , and neuropilin 1 in synovial tissues of rheumatoid arthritis . ^^^ This study examined the expression of vascular endothelial growth factor ( VEGF ) isoforms and VEGF receptors , Flt 1 , KDR and neuropilin 1 , in RA and osteoarthritis ( OA ) synovia , and studied the relationship between their expression and the synovial angiogenesis . ^^^ The receptor Flt 1 was almost constitutively expressed in RA ( 15 / 17 patients ) and OA ( 8 / 8 patients ) synovia , while the expression of KDR was detected in the synovia of six RA patients ( 6 / 17 patients ; 35 % ) but none of the OA patients ( 0 / 8 patients ) . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
After 1 week in culture the cells showed a clear expression of endothelial cell markers , including von Willebrand factor ( vWF ) , CD 144 ( VE cadherin ) , CD 105 ( endoglin ) , acetylated low density lipoprotein ( AC LDL ) receptor , CD 36 ( thrombospondin receptor ) , FLT 1 , which is vascular endothelial cell growth factor ( VEGF ) receptor 1 , and , to a weaker extent , KDR ( VEGF receptor 2 ) . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
PURPOSE : To help further define the possible role of vascular endothelial growth factor ( VEGF ) in the pathogenesis of corneal neovascularization , the expression of VEGF and of its receptors Flt 1 and Flk 1 was investigated in various inflammatory corneal diseases . ^^^ Expression of both VEGF receptors , Flt 1 and Flk 1 , was increased on endothelial cells of newly formed vessels in the stroma of inflamed corneas compared with limbal vessels of normal control corneas . ^^^ In addition , Flt 1 was also expressed by corneal endothelial cells and by macrophages , whereas Flk 1 expression was lacking . ^^^ CONCLUSIONS : These results demonstrate that VEGF , Flt 1 , and Flk 1 are strongly expressed in inflamed and vascularized human corneas and , thus , may play an important role in corneal neovascularization . . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Like VEGF , it is a potent angiogenic factor ; however , PlGF homodimers interact with the VEGF receptor Flt 1 ( fms like tyrosine kinase ) , but not with the kinase domain containing region ( KDR ) . ^^^ Expression of mRNA for the receptors of VEGF and PlGF ( KDR and flt 1 ) was determined using the reverse transcriptase polymerase chain reaction . ^^^ The SGHPL 4 cells expressed mRNA for the flt 1 but not the KDR receptor . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
This can be explained by the upregulation of the VEGF receptors ( KDR & flt 1 ) by thrombin treatment . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
We investigated the properties of two VEGF receptors , Flt 1 and KDR , by using two newly developed blocking monoclonal antibodies ( mAbs ) , i . e . , anti human Flt 1 mAb and anti human KDR mAb . ^^^ This induction was mediated by the KDR / Flt 1 heterodimer and the KDR homodimer . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
At the molecular level , brief exposure of endothelial cells to thrombin causes an upregulation of the receptors ( KDR and Flt 1 ) of VEGF , the key angiogenic mediator . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
VEGF also enhanced tube formation in dog endothelial cells and increased the expression of two VEGF receptors , Flt 1 and KDR / Flk 1 . ^^^ VEGF also increases the expression of the receptors , KDR and Flt 1 , and activates the p44 / 42 MAP kinase pathway . . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Vascular endothelial growth factor ( VEGF ) and its receptors , VEGF receptor 1 ( VEGF R 1 ; FLT 1 ) and VEGF R 2 ( KDR ) , have been shown to play a major role in tumor angiogenesis . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
VEGF and its receptors flk 1 and flt 1 are the main actors of the morphogenic phase whereas PDGF BB and its receptor PDGF R beta , TGF beta 1 and its receptors ALK 5 and ALK 1 , angiopoietin 1 and its receptor tie 2 are implied in the vascular maturation phase . . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
STI 571 was found to potently inhibit the kinase activity of the alpha and beta PDGF receptors and the receptor for stem cell factor , but not the closely related c Fms , Flt 3 , Kdr , Flt 1 , and Tek tyrosine kinases . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Vascular endothelial growth factor induces expression of connective tissue growth factor via KDR , Flt 1 , and phosphatidylinositol 3 kinase akt dependent pathways in retinal vascular cells . ^^^ VEGF activated the tyrosine receptor phosphorylation of KDR and Flt 1 and increased the binding of phosphatidylinositol 3 kinase ( PI 3 kinase ) p 85 subunit to KDR and Flt 1 , both of which could mediate CTGF gene induction . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Using immunohistochemical and in situ hybridization methods we have here demonstrated that VEGF and its receptors Flk 1 , Flt 1 and Neuropilin 1 mRNAs and proteins are induced after incisions in the rat spinal cord . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Although BREC expressed Flt 1 and Flk 1 as VEGF receptors at similar levels , VEGF stimulation preferentially enhanced the activity of Flt 1 tyrosine kinase . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Dot blot analysis showed that the expressed human VEGF 165 could bind to its receptors flt 1 and KDR . . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
METHODS : VEGF , KDR / flk 1 , and flt 1 expression were examined by immunohistochemistry , in situ hybridization , reverse transcription polymerase chain reaction , enzyme linked immunosorbent assay , and receptor phosphorylation . ^^^ VEGF expression was observed in all human pancreatic tumor cell lines examined , and the KDR / flk 1 and flt 1 receptor was detected in 2 cell lines . ^^^ KDR and flt 1 were detected not only in endothelial cells but also in tumor cells . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Immunohistochemistry revealed that VEGF was localized in luteal cells and both flt 1 and KDR were also localized in luteal cells , in addition to vascular endothelial cells . ^^^ Messenger RNA ( mRNA ) expression of VEGF , flt 1 , and KDR remained constant in the CL during the luteal phase and was lower in the regression phase . ^^^ In the pregnant CL , VEGF mRNA expression was higher compared with that in the midluteal phase , and mRNA expression of both flt 1 and KDR was the same as that in the midluteal phase . ^^^ Western blot analyses revealed that the change in protein expression of VEGF , flt 1 , and KDR was similar to that in their mRNA expression . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
In situ hybridization of fetal lung tissue at all gestational ages showed no difference in the expression of vascular endothelial growth factor , Flk 1 , or Flt 1 mRNAs . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Vascular endothelial growth factor ( VEGF ) specific Flk 1 and Flt 1 tyrosine phosphorylation was observed at Day 4 . 5 by receptor immunoprecipitation and requisite immunoblotting . ^^^ Normal differentiation of the restrictive CAM endothelial barrier at Day 5 . 0 was associated with reduced Flk 1 and Flt 1 expression , but sustained tyrosine phosphorylation of the residual RTKs . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
We report here the expression and modulation of VEGF and its receptors , Flk 1 / KDR and Flt 1 , in the functionalis throughout the menstrual cycle . ^^^ The localization of VEGF on the endothelium correlates with the presence of Flt 1 and Flk 1 / KDR receptors on vascular structures , including capillary strands that have not yet formed a lumen and that have been previously described in tumors as angiogenic capillaries . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
METHODS : VEGF , kinase domain region ( KDR ) / fetal liver kinase 1 ( flk 1 ) , and flt 1 expression were examined by immunohistochemistry and in situ hybridisation in 15 human HCC tissues . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Both primary and metastatic melanoma cells were found to express the mRNAs encoding for vascular endothelial growth factor and placenta growth factor receptors ( KDR , Flt 1 , neuropilin 1 , and neuropilin 2 ) , and exposure of melanoma cells to these cytokines resulted in a specific proliferative response , supporting the hypothesis of a role of these angiogenic factors in melanoma growth . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Vascular development in the implants was evaluated by mRNA expression of Flt 1 and KDR , two receptors for vascular endothelial growth factor ( VEGF ) . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
The expression of the signalling VEGF receptors R 1 ( flt 1 ) and R 2 ( flk 1 ) was restricted to the endothelial cells of the cortex whereas neuropilin 1 was expressed by both endothelial and steroidogenic cells . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
The molecular regulation of these distinct mechanisms is discussed in respect to the most important positive regulators , vascular endothelial growth factor ( VEGF ) and its receptors flk 1 ( KDR ) and flt 1 , the Angiopoietin / tie system and the ephrin B / EpH B system . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
To address this issue , recombinant adenoviruses encoding angiostatin , endostatin , and the ligand binding ectodomains of the vascular endothelial growth factor receptors Flk 1 , Flt 1 , and neuropilin were generated and used to systemically deliver the different gene products in several different preexisting murine tumor models . ^^^ Single i . v . injections of viruses encoding soluble forms of Flk 1 or Flt 1 resulted in approximately 80 % inhibition of preexisting tumor growth in murine models involving both murine ( Lewis lung carcinoma , T 241 fibrosarcoma ) and human ( BxPC 3 pancreatic carcinoma ) tumors . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Vascular permeability factor ( VPF ) / vascular endothelial growth factor ( VEGF ) achieves its multiple functions by activating two receptor tyrosine kinases , Flt 1 ( VEGF receptor 1 ) and KDR ( VEGF receptor 2 ) , both of which are selectively expressed on primary vascular endothelium . ^^^ To dissect the respective signaling pathways and biological functions mediated by these receptors in primary endothelial cells with these two receptors intact , we developed a chimeric receptor system in which the N terminus of the epidermal growth factor receptor was fused to the transmembrane domain and intracellular domain of KDR ( EGDR ) and Flt 1 ( EGLT ) . ^^^ We observed that KDR , but not Flt 1 , was responsible for VPF / VEGF induced human umbilical vein endothelial cell ( HUVEC ) proliferation and migration . ^^^ Moreover , Flt 1 showed an inhibitory effect on KDR mediated proliferation , but not migration . ^^^ We also observed that , in VPF / VEGF stimulated HUVECs , the Flt 1 / EGLT mediated down modulation of KDR / EGDR signaling was at or before intracellular Ca ( 2+ ) mobilization , but after KDR / EGDR phosphorylation . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
The three loci , designated Mmtg 1 3 , are unlinked to the angiogenic genes Fgf 2 , Flt 1 , Flk 4 , Flk 1 , Vegf , and Vegfc , as well as the precursors of the endogenous antiangiogenic molecules angiostatin and endostatin . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Vascular endothelial growth factor ( VEGF ) and its receptors , Flt 1 and flk 1 ( KDR ) , constitute an important angiogenic pathway which , under hypoxic conditions , is up regulated in many solid tumours . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Prognostic value of vascular endothelial growth factor and its receptors Flt 1 and Flk 1 in astrocytic tumours . ^^^ METHOD : We examined immunohistochemically expressions of VEGF and its corresponding receptors Flt 1 and Flk 1 in a series of 50 astrocytic tumours . and correlated their expressions with the degree of angiogenesis , brain edema and prognosis . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
The promoters for human Flk 1 ( fetal liver kinase 1 ) , Flt 1 ( fms like tyrosine kinase ) , ICAM 2 ( intercellular adhesion molecule 2 ) , thrombomodulin and vWf ( von Willebrand factor ) supported similar levels of luciferase expression in human and porcine aortic endothelial cells , with the Flk 1 promoter being the strongest followed by the thrombomodulin promoter . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
In this study , we tested whether vascular smooth muscles cells ( VSMCs ) can express VEGF receptors , such as flk 1 , flt 1 , and neuropilin ( NP ) 1 , and respond to VEGF in vitro . ^^^ In cultured VSMCs , flk 1 and flt 1 expression was inversely related to cell density . ^^^ Flk 1 , Flt 1 , and NP 1 protein levels were confirmed by Western Blotting . ^^^ Since VEGF E is a specific activator of flk 1 while PlGF specifically activates only flt 1 , SMC migration induced by VEGF ( 165 ) is likely to be mediated primarily through the flk 1 receptor . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Previously , it was shown that vascular endothelial growth factor and the vascular endothelial growth factor receptors KDR ( Flk 1 ) and Flt 1 are expressed in both internal thoracic arteries and saphenous veins and that vascular endothelial growth factor receptor density was higher in internal thoracic arteries than in saphenous veins . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Previous studies have demonstrated the expression of VEGF and its receptors , flt 1 or KDR / flk 1 , in hemangiosarcomas by immunohistochemical staining and in situ hybridization . ^^^ In the present study , however , we demonstrated that tumor cells of the hemangiosarcoma cell line ISO HAS express mRNA of VEGF and its two receptors , flt 1 and KDR , and secrete VEGF protein . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
We examined the regulation of the expression of vascular endothelial growth factor ( VEGF ) and its specific receptors , fetal liver kinase receptor ( Flk 1 ) , and fms like tyrosine kinase receptor ( Flt 1 ) during formation of the capillary network in the developing rat lung . ^^^ Immunostaining also revealed the colocalization of VEGF , Flt 1 , and Flk 1 in endothelial cells of the lung capillaries at the ultrastructural level . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Two VEGF receptors , KDR ( kinase domain region ) and Flt 1 ( fms like tyrosine kinase ) are co expressed by glomerular and peritubular capillary endothelial cells . ^^^ In the present work we focused on the tubulo interstitial compartment ; by reverse transcription / polymerase chain reaction ( RT / PCR ) we evaluated the expression of VEGF , KDR , Flt 1 and the relationship between the two main type of VEGF isoforms , VEGF 121 and VEGF 165 in the tubulo interstitium of type 2 diabetic patients . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Therefore , we examined the effects of exercise training ( treadmill running at the same absolute intensity ) in normoxia and hypoxia ( inspired O ( 2 ) fraction = 0 . 12 ) on rat skeletal muscle capillarity and on resting and postexercise gene expression of VEGF , its major receptors ( flt 1 and flk 1 ) , TGF beta ( 1 ) , and bFGF . ^^^ Rats trained in hypoxia exhibited an attenuated VEGF mRNA response to exercise ( 1 . 8 fold compared 3 . 4 fold with normoxic training ; P < 0 . 05 ) , absent TGF beta ( 1 ) and flt 1 mRNA responses to exercise , and an approximately threefold ( P < 0 . 05 ) decrease in bFGF mRNA levels . flk 1 mRNA levels were not significantly altered by either normoxic or hypoxic training . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Moreover , enhanced endothelial cell proliferation induced by conditioned medium of macrophages isolated from the ascites of patients with SBP is abolished by anti VEGF antibody , and peritoneal tissue of cirrhotic patients expresses both VEGF receptors , Flt 1 and KDR . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Correspondingly , the VEGF C specific receptor flt 4 and the VEGF A receptors flt 1 and flk 1 were up regulated in a temporal sequence similar to that of their agonist proteins in the cortical ring lesion and the region at risk . ^^^ This study suggests that VEGF C and its receptor flt 4 may cooperate with VEGF A and its receptors flt 1 and flk 1 to promote early angiogenesis after stroke , which may in turn contribute to spontaneous reperfusion in this focal thromboembolic stroke model . . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Vascular endothelial growth factor ( VEGF ) binds to and mediates its activity mainly through two tyrosine kinase receptors , VEGF receptor 1 [ or fms like tyrosine kinase receptor ( Flt 1 ) ] and VEGF receptor 2 [ or kinase insert domain containing receptor ( KDR ) ] . ^^^ Here we constructed a recombinant bifunctional diabody that is capable of blocking both Flt 1 and KDR from binding to their ligands , including VEGF and placenta growth factor ( PlGF ) . ^^^ The diabody retained the capacity to bind both KDR and Flt 1 and effectively blocked interaction between KDR and VEGF , Flt 1 and VEGF , and Flt 1 and PlGF . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
The anilino phthalazine derivative PTK787 / ZK222584 ( Phase 1 , co developed by Schering AG , Berlin ) is a potent and selective inhibitor of both the KDR and Flt 1 kinases with interesting anti angiogenic and pharmacokinetic properties ( orally bioavailable ) . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
In the present study , we analyzed the expression of VEGF and its receptors ( Flk 1 and Flt 1 ) in a rabbit model of collateral artery growth after femoral artery occlusion . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
In order to study basic factors related to angiogenesis and phenotypic changes of the capillaries located in tumor bearing alveolar walls , we examined 37 primary lung adenocarcinomas with electron microscopy and confocal laser scanning microscopy with antibodies for TM , vWf , vascular endothelial growth factor ( VEGF ) , and its receptors ( KDR and Flt 1 ) , and proliferating markers ( Ki 67 / proliferating cell nuclear antigen ) . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
The aim of this study was to investigate the expression of vascular endothelial growth factor ( VEGF ) receptors , the fms like tyrosine kinase ( flt 1 ) and kinase insert domain containing region ( KDR ) , in corpora lutea obtained at different stages of the oestrous cycle and during pregnancy in rats . ^^^ Immunohistochemistry revealed that both flt 1 and KDR were localized in luteal cells in addition to vascular endothelial cells , and that the intensity of staining was stronger in pregnant rats than in cyclic rats . ^^^ Rats undergoing hypophysectomy hysterectomy on day 12 of pregnancy were treated with oestradiol until day 15 of pregnancy to determine whether oestradiol is involved in expression of flt 1 and KDR mRNA in the corpus luteum during mid pregnancy . ^^^ The flt 1 and KDR mRNA contents in the corpus luteum were decreased significantly by hypophysectomy hysterectomy , and these decreases recovered significantly after oestradiol treatment . ^^^ Changes in the mass of the corpus luteum and serum progesterone concentrations paralleled the changes in expression of flt 1 and KDR mRNA . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
KDR , Flt 1 , Tie 1 , Tie 2 , and angiopoietin 2 ( Ang 2 ) were strongly expressed in cultured hemangioma derived endothelial cells and in hemangioma tissue . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
While expression of the Flt 1 and KDR receptors was not observed in the malignant plasma cells , both were markedly elevated in the normal marrow myeloid and monocytic cells surrounding the tumor . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Vascular endothelial cell growth factor ( VEGF ) binds to 2 related receptor tyrosine kinases , known as kinase insert domain containing receptor ( KDR ) and fms like tyrosine kinase ( Flt 1 ) . ^^^ In the present study , we have used 2 independent endothelial cell tube formation models and highly selective VEGF mutants for the KDR and Flt 1 receptors . ^^^ We demonstrate that KDR , not Flt 1 , stimulation is responsible for the induction of endothelial tubulogenesis . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
METHODS : The expression and localization of the mRNA of VEGF and its receptors , flt 1 and flk 1 , were analyzed in the kidneys of puromycin aminonucleoside injected rats by use of Northern blotting and in situ hybridization . ^^^ The levels of flk 1 and flt 1 mRNA showed similar transient decrease in PAN kidney , whereas the mRNA of von Willebrand factor , a marker of endothelial cells , showed no change in amount . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Blockade of in vivo VEGF mediated angiogenesis by antisense gene therapy : role of Flk 1 and Flt 1 receptors . ^^^ Vascular endothelial growth factor ( VEGF ) is a mitogenic and chimiotactic factor capable of inducing angiogenesis through the activation of its receptors , fetal liver kinase 1 ( Flk 1 ) and fms like tyrosine kinase 1 ( Flt 1 ) , expressed on endothelial cells . ^^^ The purpose of the present study was to assess if a treatment with antisense ( AS ) oligonucleotides directed against VEGF receptors Flk 1 or Flt 1 mRNA could prevent VEGF mediated angiogenesis . ^^^ With the use of miniosmotic pumps , phosphate buffered saline , VEGF , or VEGF combined with AS Flk 1 , AS Flt 1 , or AS scrambled oligonucleotides were released in mouse testis for 14 days . ^^^ The combination of AS Flk 1 or AS Flt 1 ( 200 microg ) to VEGF ( 2 . 5 microg ) reduced by 87 and 85 % the formation of new blood vessels , respectively , and the expression of their corresponding proteins . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
This paper studies the immunohistochemical expression of vascular endothelial growth factor ( VEGF ) and its receptors Flt 1 and Flk 1 , and basic fibroblast growth factor ( bFGF ) in BOOP and UIP . ^^^ The expression of Flt 1 and Flk 1 was in agreement with the expression of VEGF . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
RT PCR analysis in the Ihh ( / ) lines shows a substantial decrease in the expression of Flk 1 and Tal 1 , markers for the hemangioblast , the precursor of both blood and endothelial cells , as well as Flt 1 , an angiogenesis marker . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Thrombin induced a significant increase in the level of mRNA of the kinase domain containing receptor ( KDR ) , but not tms like tyrosine kinase ( Flt 1 ) , in a time dependent manner , which reached the maximum after 24 h of stimulation . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
BACKGROUND : Recent studies show that vascular endothelial growth factor ( VEGF ) and its receptors Flt 1 and KDR , and a series of other angiogenic molecules , are upregulated in advanced but not low stage human neuroblastoma . ^^^ The mRNA expression of VEGF ( 165 ) and its receptors Flt 1 , KDR , NRP 1 , and NRP 2 was evaluated by real time reverse transcription polymerase chain reaction . ^^^ RESULTS : VEGF ( 165 ) mRNA was upregulated in Stage 3 and 4 and Flt 1 and KDR gene expression was increased in Stage 3 , while NRP 1 and 2 mRNA and protein levels were higher in Stages 1 4 vs . controls ( P < 0 . 05 ) . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
METHODS AND RESULTS : We investigated the expression of VEGF and its receptors ( flt 1 , KDR ) in acute KD tissues at 7 days to 5 weeks of illness . ^^^ Compared with controls , coronary vessels of acute KD had upregulation of VEGF and flt 1 but not KDR or neuropilin 1 . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Vascular endothelial growth factor ( VEGF ) , transforming growth factor beta ( 1 ) ( TGF beta ( 1 ) ) , basic fibroblast growth factor ( bFGF ) , Flt 1 , and Flk 1 mRNA were analyzed from the left gastrocnemius by quantitative Northern blot . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
The aim of this study was to examine immunohistochemically the presence of the VEGF family ( VEGF A to D ) and their receptors ( Flt 1 , Flk 1 , and Flt 4 ) in the surgically resected AVM nidus . ^^^ Formalin fixed , paraffin embedded specimens were stained immunohistochemically by the labeled streptavidin biotin method with antibodies against VEGF A to D , as well as Flt 1 , Flk 1 , and Flt 4 . ^^^ Flt 1 , Flk 1 , and Flt 4 were also positive chiefly , but not exclusively , in the cytoplasm of vascular endothelium and smooth muscle cells of the vascular wall . ^^^ With regard to VEGF receptors , it was found that among the 31 patients studied , 19 ( 61 . 3 % ) were immunohistochemically positive for Flt 1 , 6 ( 19 . 4 % ) for Flk 1 , and 19 ( 61 . 3 % ) for Flt 4 . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Three classes of disulfide constrained peptides that antagonize binding of the VEGF dimer to its receptors , KDR and Flt 1 , were identified previously using phage display methods . ^^^ The v 107 binding site on VEGF overlaps partially with the binding site of KDR and is similar to that for domain 2 of Flt 1 . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
We have previously shown that 4 anilinoquinazolines can be potent inhibitors of vascular endothelial growth factor ( VEGF ) receptor ( Flt 1 and KDR ) tyrosine kinase activity . ^^^ This subseries contains potent , nanomolar inhibitors of KDR ( median IC ( 50 ) 0 . 02 microM , range 0 . 001 0 . 04 microM ) , which are comparatively less potent vs Flt 1 tyrosine kinase ( median IC ( 50 ) 0 . 55 microM , range 0 . 02 1 . 6 microM ) . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Immunohistochemistry for vascular endothelial growth factor ( VEGF ) and its receptors , fms like tyrosine kinase ( flt 1 ) and kinase insert domain containing region ( KDR ) , was performed on human endometrium obtained from patients with normal menstrual cycles , patients given oestrogen and progesterone , and women in early pregnancy . ^^^ In addition to the immunostaining in vascular endothelial cells , strong KDR immunostaining was observed in glandular epithelial cells and in decidualized stromal cells induced by administration of oestrogen plus progesterone , whereas flt 1 immunostaining was negligible . ^^^ Strong immunostaining for flt 1 and KDR was found in both vascular endothelial cells and decidual cells in early pregnancy . ^^^ Endometrial stromal cells isolated from proliferative phase endometrium were incubated with oestrogen ( 10 ( 8 ) mol l 1 ) and medroxyprogesterone acetate ( MPA ; 10 ( 6 ) mol l 1 ) for 18 days to study the regulation of VEGF , flt 1 and KDR in endometrial stromal cells by oestrogen and progesterone . ^^^ Expression of VEGF and KDR mRNAs was increased significantly by oestrogen and MPA , accompanied by decidualization , whereas flt 1 mRNA expression was not affected . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Vascular endothelial growth factor ( VEGF ) acts primarily as an endothelial cell mitogen via the specific receptors Flk 1 and Flt 1 . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
A significant inhibition of the angiogenic effect of PAF was achieved by anti VEGF antibodies or soluble VEGF receptors such as KDR and flt 1 but not by antibodies against tumor necrosis factor alpha , interleukin 1alpha , or basic fibroblast growth factor . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Vascular endothelial growth factor ( VEGF ) is a major regulator of angiogenesis that binds to tyrosine kinase receptors Flt 1 and KDR . ^^^ VEGF A ( 165 ) , B , C , Flt 1 , and KDR were analyzed . ^^^ At protein level , VEGF B and C , Flt 1 , and KDR were not detectable in tissue lysates , whereas VEGF A was increased in stages 3 and 4 . ^^^ CONCLUSIONS : VEGF A ( 165 ) is one of the major angiogenesis regulators among the ligands ' family of VEGF , whereas its receptors KDR , and most probably Flt 1 , may contribute to a poor prognosis ( angiogenic ) phenotype , as indicated by their upregulated MRNA levels in stage 3 neuroblastoma . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Immunohistochemical results and semi quantitative reverse transcriptase polymerase chain reaction indicated that the VEGF receptors flk 1 and flt 1 were up regulated in response to the infusion trauma ; flt 1 was localized to reactive astroglia , while flk 1 was expressed in vascular endothelium but predominantly in neuronal somata and processes adjacent to the delivery site . mRNA for the VEGF ( 121 ) , VEGF ( 165 ) and VEGF ( 188 ) isoforms was also increased after delivery of the recombinant protein . ^^^ Flt 1 and flk 1 receptor mRNAs and proteins are up regulated in both vascular and non vascular cell types following infusion trauma . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Tumors removed at 1 week intervals up to week 6 were probed by immunohistochemistry ( n = 3 11 samples ) for vascular endothelial growth factor ( VEGF ) , placental growth factor ( PlGF ) , flk 1 and flt 1 , angiopoietin 1 and 2 , and Tie 1 and 2 . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Design of a variant of vascular endothelial growth factor A ( VEGF A ) antagonizing KDR / Flk 1 and Flt 1 . ^^^ In this antagonist , binding domains for the VEGF receptors KDR / Flk 1 and Flt 1 are present at one pole of the dimer , whereas the other pole carries domain swap mutations , which prevent binding to either receptor . ^^^ Moreover , it antagonizes VEGF and possibly other members of the VEGF family , which are KDR / Flk 1 and Flt 1 ligands . ^^^ Thus , HD VEGF blocks KDR and Flt 1 mediated VEGF activities that are crucial in the angiogenic process and is therefore a promising , multipotent compound in the treatment of angiogenesis related diseases . . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Immunohistochemistry on paraffin sections was performed with anti human factor 8 antibody to study the microvascular density ( MVD ) , and with antibodies to VEGF , Flt 1 , and KDR to investigate the expression of these three proteins in different cellular compartments . ^^^ Survival analysis showed that the high MVD group was associated with a high risk of death . ( 3 ) There was no significant difference between low MVD and high MVD groups in VEGF expression in tumor cells , but in endothelial cells both Flt 1 and KDR were correlated with high microvessel count . ( 4 ) The high expression of VEGF in tumor cells was correlated with that in stromal fibroblasts . ^^^ The level of expression in both cells was consistent . ( 5 ) In both tumor cells and endothelial cells , high co expression of VEGF and KDR was consistent , but that of VEGF and Flt 1 showed inconsistency . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
To assess the contribution of NRP 1 to VEGF A mediated EC proliferation , migration , and PAF synthesis , we used porcine aortic EC ( PAEC ) recombinantly expressing Flt 1 , NRP 1 , KDR or KDR and NRP 1 . ^^^ Cells were stimulated with VEGF A , which binds to Flt 1 , KDR and NRP 1 , and VEGF C , which binds to KDR only . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Using quantitative RT PCR , VEGF and its receptors , Flk 1 and Flt 1 , were detected in CK MVECs . ^^^ The mRNA expression of Flk 1 mRNA was 100 times as high as that of Flt 1 mRNA . ^^^ CK+ MVECs expressed VEGF and Flt 1 mRNA , but were devoid of Flk 1 transcripts . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Small molecules that selectively inhibit the VEGF receptor associated tyrosine kinase activities of Flk 1 ( KDR ) and Flt 1 have been developed . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Many cellular effects of thrombin on endothelial cells can contribute to the angiogenic action of thrombin . ( 1 ) Exposure of endothelial cells to thrombin cause a time and dose dependent decrease in the attachment of these cells to basement membrane components , with a concomitant increase in matrix metalloproteinase 2 activation . ( 2 ) Thrombin upregulates the expression of integrin alphavbeta 3 , the marker of the angiogenic phenotype of endothelial cells . ( 3 ) Thrombin has chemotactic and aptotactic effects on endothelial cells and upregulates the expression of the vascular endothelial growth factor ( VEGF ) receptors ( KDR and Flt 1 ) . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
VEGF has 2 receptor tyrosine kinases , KDR and Flt 1 . ^^^ To elucidate the role of each receptor in mediating hypotension , KDR selective and Flt 1 selective mutants were used for in vitro and in vivo studies . ^^^ The KDR selective mutant induced vascular endothelial cell proliferation comparable to VEGF , whereas the Flt 1 selective mutant had no effect on proliferation . ^^^ Similarly , VEGF and KDR selective mutant induced more potent vasorelaxation than Flt selective mutant or placenta growth factor that binds Flt 1 only ( P < 0 . 01 ) , and the vasorelaxation to KDR selective mutant was not significantly different at low concentrations but less than that to VEGF at high concentrations . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
A neoangiogenesis and blood cell gene cluster included LCK , HCK , FGR , MMP 9 , CSFR 1 , VEGF , FLT 1 , and KDR . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Flt 1 was unchanged , KDR gene expression was down regulated in donors ( P < 0 . 05 ) , and both receptors ' protein expression was under the detection level in donor and control lungs . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Gene expression of adhesion molecules , interleukin 1alpha ( IL 1alpha ) , IL 1beta , monocyte chemoattractant protein 1 , macrophage inflammatory protein 1alpha ( MIP 1alpha ) , MIP 2 , transforming growth factor beta 1 ( TGF beta 1 ) connective tissue growth factor ( CTGF ) , vascular endothelial growth factor ( VEGF ) , Flt 1 , and Flk 1 was enhanced at the wound site . ^^^ In KO mice , an enhancement in angiogenesis , collagen content , and reepithelialization was accelerated with the increased gene expression of TGF beta 1 , CTGF , VEGF , Flt 1 , and Flk 1 at the wound sites , resulting in accelerated wound healing compared with WT mice . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
We investigated the vascular endothelial growth factor ( VEGF ) receptor [ fms like tyrosine kinase ( Flt 1 and fetal liver kinase 1 ( Flk 1 ) ] response to acute exercise . ^^^ Exercise altered both Flt 1 and Flk 1 mRNA , with significant increases in Flt 1 mRNA at 1 and 24 h . ^^^ These findings suggest that : ( 1 ) exercise alters Flt 1 and Flk 1 gene expression ; and ( 2 ) NO is important in the regulation of the Flt 1 gene response to exercise . . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
In both models , expression of VEGF correlated with expression of mRNAs encoding other angiogenic cytokines ( angiopoietin 1 and angiopoietin 2 ) , endothelial cell receptor tyrosine kinases ( Flt 1 , KDR , Tie 1 ) , and endothelial cell adhesion molecules ( VE cadherin , PECAM 1 ) . ^^^ Relative to control , in dermis highly stimulated by VEGF , the Ang 2 mRNA transcript numbers increased 35 fold , PECAM 1 and VE cadherin increased 10 fold , Tie 1 increased 8 fold , KDR and Flt 1 each increased 4 fold , and Ang 1 increased 2 fold . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
The aim of the present study was to evaluate the expression of VEGF and of its two high affinity tyrosine kinase receptors ( KDR and Flt 1 ) in neuroblastoma surgical samples and cell lines . ^^^ PROCEDURE : The VEGF , KDR , and Flt 1 mRNA expression in neuroblastoma surgical samples and cell lines was studied by RT PCR . ^^^ It was also possible to demonstrate that the SK N BE cell line expressed VEGF , KDR , and Flt 1 mRNAs as well as biologically active receptors : The cells bound [ ( 125 ) 1 ] VEGF , and their growth was stimulated by exogenous VEGF . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Expression of Flk 1 , but not Flt 1 , was increased in embryonic aorta cultured under 5 % O ( 2 ) relative to room air . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
VEGF acts via two high affinity receptors : KDR and FLT 1 . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
During early fetal angiogenesis , vascular endothelial growth factor ( VEGF ) and its receptors kinase insert domain containing receptor ( KDR ) and fms like tyrosine kinase 1 ( Flt 1 ) are required for the development of the systemic vasculature . ^^^ KDR and Flt 1 levels were high during the GA , the level being significantly higher than those during the GH and GS ( P < 0 . 05 ) . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Our prior studies show that multiple myeloma ( MM ) cell lines and patient cells express high affinity vascular endothelial growth factor ( VEGF ) receptor ( VEGFR ) Flt 1 but not Flk 1 / KDR . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
These SOCs were positive for smooth muscle cell specific alpha actin ( alphaSMA ) , myosin heavy chain , and calponin on immunofluorescence and Western blotting and were also positive for CD 34 , Flt 1 , and Flk 1 receptor but negative for Tie 2 receptor expression , suggesting a potential bone marrow angioblastic origin . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
The signaling of AMF AMFR in the host endothelial cells induces expression of a vascular endothelial growth factor receptor ( VEGFR ) Flt 1 and AMFR feedback that is regulated at the transcriptional level . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
The VEGF receptors fetal liver kinase 1 ( Flk 1 ) and Fms like tyrosine kinase 1 ( Flt 1 ) become highly expressed in epithelial tumor and endothelial cells in the mammary carcinomas , suggesting a potential autocrine effect for VEGF on tumor cell growth . ^^^ However , mRNA levels of a variety of proangiogenic factors ( VEGF , VEGF receptors Flk 1 and Flt 1 , angiopoietin 2 , Tie 1 , cadherin 5 and PECAM ) were significantly decreased in the endostatin treated group compared with controls . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
In this study we address this issue by using a novel in vivo method utilizing fibrin containing dual porous plexiglass chambers ( Fibrin Z Chambers ; F ZC ) to investigate wound healing in rats administered with SU 5416 ( inhibitor of Flk 1 and Flt 1 , at 20 mg / kg i . p . ) . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
When CD34+ cells were isolated from midtrimester human fetal lung tissue , then maintained in culture , the isolated cells expressed immunoreactivity for the endothelial cell marker von Willebrand factor and the vascular endothelial growth factor receptors KDR and Flt 1 . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
We have investigated the expression of Flt 1 and kinase domain receptor ( KDR ) on hematopoietic precursors , as evaluated in liquid culture of CD 34 ( + ) hematopoietic progenitor cells ( HPCs ) induced to unilineage differentiation / maturation through the erythroid ( E ) , megakaryocytic ( Mk ) , granulocytic ( G ) , or monocytic ( Mo ) lineage . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Vascular endothelial growth factor induced genes in human umbilical vein endothelial cells : relative roles of KDR and Flt 1 receptors . ^^^ OBJECTIVE : This study evaluated the relative roles of the vascular endothelial growth factor ( VEGF ) receptors KDR and Flt 1 in the mediation of altered gene expression elicited by VEGF . ^^^ METHODS AND RESULTS : We used mutants of VEGF selective for the KDR and Flt 1 receptors to differentiate gene expression patterns mediated by wild type VEGF ( VEGFwt ) in human umbilical vein endothelial cells . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Western blot analysis also indicated that there is no difference in the levels of proteins which are directly involved in angiogenesis , such as VEGF , Flt 1 , and Flk 1 , between cells transfected with vector , p33ING1 , and antisense p33ING1 . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Expression of vascular endothelial growth factor and its receptors KDR and Flt 1 in gastric cancer cells . ^^^ AIM : The expression of vascular endothelial growth factor ( VEGF ) and its receptors KDR and Flt 1 by gastric carcinoma tissues and different gastric carcinoma cell lines was detected to elucidate the molecular mechanism of this growth factor in promoting tumor growth . ^^^ METHODS : The expression of VEGF , Flt 1 and KDR was determined by reverse transcription polymerase chain reaction ( RT PCR ) in gastric cancer cell lines RF 1 , RF 48 , AGS 1 , NCI N 87 , NCI SNU 1 , NCI SNU 5 , NCI SNU 16 and KATO 3 . ^^^ The expression of Flt 1 and KDR in paraffin embedded specimens of gastric cancer was determined by immunohistochemistry . ^^^ RESULTS : All 8 gastric cancer cell lines analyzed expressed VEGF ( 121 ) and VEGF ( 165 ) and six of them expressed both Flt 1 and KDR , while cell line NCI SNU 5 expressed Flt 1 only and cell line KATOIII expressed neither Flt 1 nor KDR . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Granulosa cells and thecal tissues in small ( diameter , < 4 mm ) , medium ( diameter , 4 5 mm ) , or large ( diameter , > 5 mm ) individual follicles were collected for detection of mRNA expression of vascular endothelial growth factor ( VEGF ) 120 , VEGF 164 , basic fibroblast growth factor ( bFGF ) , and epidermal growth factor ( EGF ) in granulosa cells and fms like tyrosine kinase ( Flt 1 ) , fetal liver kinase ( Flk 1 ) or the murine homologue of kinase domain region ( KDR ) , bFGF receptor ( bFGF R ) , and EGF receptor ( EGF R ) in thecal tissue by semiquantitative reverse transcription polymerase chain reaction . ^^^ The Flt 1 , Flk 1 / KDR , and bFGF R mRNA expression increased in theca cells of medium and large follicles after eCG treatment . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Differentiation and migration activities of endothelial precursor cells were measured by comparing levels of the endothelial precursor cell maturation markers Flk 1 , Flt 1 , Tie 2 , VE cadherin and CD 31 among these three areas . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Expression of vascular endothelial growth factor , placental growth factor , and their receptors Flt 1 and KDR in human placenta under pathologic conditions . ^^^ We have investigated the expression patterns of VEGF , placental growth factor ( PlGF ) , and their receptors fms like tyrosine kinase ( Flt 1 ) and kinase insert domain containing region ( KDR ) in placentas with histopathological changes . ^^^ These findings suggested that in the hypoxic / ischemic regions , VEGF and KDR expression is increased within the villous vessels by paracrine regulation , whereas the expression of PlGF and Flt 1 is enhanced in villous trophoblasts by autocrine regulation . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Furthermore , expression of many angiogenic genes including those for vascular endothelial growth factor , fibroblast growth factor , platelet derived growth factor , and receptors such as Flt 1 , Flk 1 , Ang 1 , and Ang 2 are likely to be regulated by redox signaling . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
The data were insufficient to determine the prognostic value of VEGF in SCLC and that of its two receptors Flt 1 and KDR , with 1 , 2 and 1 published studies respectively . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
OBJECTIVE : To study the expression of vascular endothelial growth factor ( VEGF ) and its receptors , the fms like tyrosine 1 ( flt 1 ) and kinase insert domain containing receptor ( KDR ) in endometrial carcinoma and investigate the functions of VEGF and its receptors for endometrial carcinoma angiogenesis and its relation to the grade of tumor . ^^^ METHODS : Immunocytochemistry and in situ hybridization technique were used to measure the level of VEGF , flt 1 , KDR protein and mRNA in endometrial carcinoma tissue from 23 patients and endometrial samples from 6 normal menopausal women . ^^^ CONCLUSIONS : The expression pattern of VEGF , flt 1 and KDR protein and mRNA increased with the increase of tumor grade in endometrial carcinoma indicates that VEGF and its receptors contribute to the neovascularization of tumors and is one of the factors that relate to rapid tumor growth of endometrial carcinoma . . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Interaction between vascular endothelial growth factor ( VEGF ) and its cognate receptors , KDR / Flk 1 and Flt 1 , of vascular endothelial cells is expected to induce an angiogenesis `` switch ' ' in tumors and other angiogenesis associated diseases . ^^^ In this study , we first observed that SU 5416 inhibited Flt 1 tyrosine kinase activity at similar doses , in addition to inhibiting KDR / Flk 1 tyrosine kinase activity in response to VEGF . ^^^ SU 5416 inhibited cell migration of human vascular endothelial cells expressing both Flt 1 and KDR in response to VEGF and also inhibited the cell migration in response to placenta growth factor ( PIGF ) , a specific ligand for Flt 1 . ^^^ The antiangiogenic effects by this VEGF receptor targeting compound appeared to be mediated through interference not only with KDR / Flk 1 but also with Flt 1 . ^^^ Cell migration of vascular endothelial cells and monocytic cells through Flt 1 , thus , might play a key role in VEGF induced tumor angiogenesis in concert with KDR / Flk 1 . . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
We evaluated the ex vivo expression of IGF 1 , VEGF , and their receptors ( IGF IR , Flt 1 , and KDR ) in human penile cavernosal smooth muscle cells ( HCSMCs ) to identify cellular and molecular pathways involved in the regulation of penile tissue vascularity . ^^^ To evaluate gene expression of VEGF , Flt 1 , and KDR , total RNA was extracted from cavernosal cells and subjected to reverse transcriptase polymerase chain reaction ( RT PCR ) using custom synthesized primers . ^^^ RT PCR evaluation revealed the expression of four splice variants of VEGF messenger RNA ( VEGFs 121 , 145 , 165 , and 189 ) and two of its receptors ( Flt 1 and KDR ) . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
This study was designed to explore the gene coexpression pattern of VEGF and its receptors ( Flt 1 and KDR ) in variety of human malignant cell lines . ^^^ However , ECV 304 cells express neither Flt 1 nor KDR gene . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
To account for reproductive failure induced by surgical deletion of paternal accessory sex glands in the golden hamster in vivo , we studied expression of vegf , FLT 1 ( VEGF R 1 ) , FLK 1 ( VEGF R 2 ) , MMP and TGF beta in endometrium of the dam and sired embryos during 5 7 days post coitum by immunohistochemistry , in situ hybridisation , semiquantitative RT PCR and enzyme linked immunosorbent assay . ^^^ FLT 1 , FLK 1 and MMP 2 were also reduced . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Vascular endothelial growth factor ( VEGF ) and its specific receptors FLT 1 and FLK 1 represent an important ligand receptor system involved in angiogenesis and permeability . ^^^ A complete VEGF system was found in endometrial tissue using RT PCR detecting the main VEGF isoform 188 aa , FLT 1 and FLK 1 . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Expression of vascular endothelial growth factor isoforms and receptors Flt 1 and KDR during the peri implantation period in the mink , Mustela vison . ^^^ Expression of vascular endothelial growth factor ( VEGF ) isoforms and its receptors , Flt 1 and KDR , was investigated during the period of peri implantation in mink , a species that displays obligate embryonic diapause . ^^^ The abundance of the two receptors , KDR and Flt 1 , increased in the uterus during implantation . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
OBJECTIVE : To detect the expression of the vascular endothelial growth factor ( VEGF ) and its receptors , the fms like tyrosine ( flt 1 ) , kinase insert domain containing receptor ( KDR ) in normal human endometrium during menstrual cycle and investigate the functions of VEGF and its receptors for development and differentiation of human endometrium . ^^^ METHODS : Immunohistochemistry and in situ hybridization techniques were used to measure the level of VEGF , flt 1 and KDR protein and mRNA in normal endometrium from 50 women . ^^^ CONCLUSIONS : VEGF , flt 1 and KDR , including both protein and mRNA , showed a pronounced menstrual cycle dependent expression in cycling endometrium . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
The aim of the present study was to investigate if the mRNA expression of vascular endothelial growth factor ( VEGF ) , Fms like tyrosine kinase 1 receptor ( Flt 1 ) and kinase insert domain containing receptor ( KDR ) is regulated by shear stress . ^^^ The mRNA expression of VEGF , Flt 1 and KDR was measured with RT PCR . eNOS served as positive control and actin as housekeeping gene . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
BACKGROUND : Vascular endothelial growth factor ( VEGF ) is involved in angiogenesis , wound healing , and inflammation and exerts its effect via tyrosine kinase receptors , fms like tyrosine kinase ( Flt 1 ) and fetal liver kinase ( Flk 1 or KDR ) . ^^^ Physiologic and histologic changes were studied in addition to the mRNA expression of VEGF and its receptors Flt 1 and KDR / Flk 1 by Northern blot and the protein expression of VEGF by Western blot and immunohistochemical staining . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Reverse transcriptase polymerase chain reaction ( RT PCR ) is utilized to measure the VEGF receptors flt 1 , KDR / flk 1 , flt 4 , neuropilin 1 ( NRP 1 ) , and neuropilin 2 ( NRP 2 ) . ^^^ RESULTS : Flt 1 and KDR are not detected in any groups . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Vascular permeability factor / vascular endothelial growth factor ( VPF / VEGF ) functions by activating two receptor tyrosine kinases , Flt 1 ( VEGF receptor ( VEGFR ) 1 ) and KDR ( VEGFR 2 ) , both of which are selectively expressed on primary vascular endothelium . ^^^ KDR is responsible for VPF / VEGF stimulated endothelial cell proliferation and migration , whereas Flt 1 down modulates KDR mediated endothelial cell proliferation . ^^^ Our most recent works show that pertussis toxin sensitive G proteins and Gbetagamma subunits are required for Flt 1 mediated down regulation of human umbilical vein endothelial cell ( HUVEC ) proliferation and that Gq / 11 proteins are required for KDR mediated RhoA activation and HUVEC migration . ^^^ Our results further indicate that Gq / 11 proteins specifically mediate KDR signaling such as intracellular Ca2+ mobilization rather than Flt 1 induced CDC 42 activation and that a Gq / 11 antisense oligonucleotide completely inhibits MAPK phosphorylation induced by KDR but has no effect on Flt 1 induced MAPK activation . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Double staining combined with confocal laser scanning microscopy was used to simultaneously detect the distribution of VEGF receptors ( flt 1 and flk 1 ) in the hippocampal section and isolated neuron . ^^^ Results showed that flt 1 positive staining , but not flk 1 , could be observed on the membrane of the hippocampal neuron in both preparations , suggesting the presence of neuronal membrane VEGF flt 1 receptors in the hippocampus . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Cardiac myofibroblasts : a novel source of vascular endothelial growth factor ( VEGF ) and its receptors Flt 1 and KDR . ^^^ VEGF actions are mediated via two major receptors , Flt 1 and KDR , and hence the expression of these receptors was investigated . ^^^ Flt 1 and KDR expression in myoFb was detected by RT PCR , RNA transcripts were confirmed by northern blot hybridization while western blot confirmed the presence of VEGF , Flt 1 and KDR proteins in myoFb . ^^^ In this study AngII upregulated VEGF and Flt 1 expression in myoFb , but not KDR ; this was mediated predominantly by AT 1 receptor . ^^^ We report for the first time that cardiac myoFb , isolated from the site of infarction express VEGF , its receptors , Flt 1 and KDR , with modulation of VEGF and Flt 1 expression by AngII . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
We performed mutational analysis of FLT 3 , c kit , c fms , vascular endothelial growth factor ( VEGF ) receptors ( Flt 1 , KDR [ kinase domain receptor ] ) , and ras genes in a group of 91 pediatric patients with AML treated on Children ' s Cancer Group clinical trial CCG 2891 . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Both VEGF receptors ( flt 1 and flk 1 ) were identified on endothelial cells of epicardial arteries and veins . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
The localization of VEGF in the endothelium correlates with the presence of Flt 1 and KDR receptors on vascular structure . ^^^ Expression of VEGF , Flt 1 , and KDR in the epithelial cells also hints some additionally functional roles of VEGF during early pregnancy . . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Differential expression of Flk 1 and Flt 1 in rat skeletal muscle in response to chronic ischaemia : favourable effect of muscle activity . ^^^ To determine the involvement of vascular endothelial growth factor ( VEGF ) and its receptors Flk 1 and Flt 1 in capillary growth in ischaemic skeletal muscle , extensor digitorum longus muscles from hindlimbs of Sprague Dawley rats were studied at 1 , 2 and 5 week intervals after iliac artery ligation . ^^^ This was associated with an initial doubling of Flk 1 protein after 1 week that declined below control levels by 5 weeks , whereas Flt 1 expression was elevated more than 40 % at all time points . ^^^ During more sustained ischaemia ( femoral ligation 3 weeks after iliac ligation ) , VEGF protein level at 5 weeks was even higher , but Flt 1 and Flk 1 were unchanged from control levels and no capillary growth occurred . ^^^ Intermittent electrical stimulation ( 10 Hz , 7x15 min / day ) of these ischaemic muscles between weeks 3 5 did not elevate VEGF further , but increased Flk 1 by 32 % , decreased Flt 1 by 71 % , and led to significant capillary growth . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Tissues adjacent to the infusion / wound site were analyzed for specific vascular and astroglial protein markers and proliferation , necrosis / apoptosis ( via TUNEL staining ) , VEGF , the VEGF receptors flt 1 and flk 1 , and bFGF expression using immunohistochemistry and semi quantitative RT PCR . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
OBJECTIVE : To study the expression of vascular endothelial cell growth factor ( VEGF ) and its receptor FLT 1 , FLK 1 mRNA in breast cancer tissues and their correlation with clinicopathological factors . ^^^ METHODS : The expression of VEGF and and its receptor FLT 1 , FLK 1 mRNA were analyzed by reverse transcription polymerase chain reaction ( RT PCR ) in the specimens from 47 patients with breast cancer and 11 patients with benign breast disease . ^^^ FLT 1 , FLK 1 mRNA were expressed in 18 of 47 ( 38 . 3 % ) and 12 of 47 ( 25 . 5 % ) breast cancer tissues respectively , but not in benign breast tissues . ^^^ Moreover , no correlation was observed between the expression of VEGF 121 , VEGF 165 , FLT 1 , FLK 1 mRNA in breast cancer tissues and patients ' age , tumor size , lymph node metastasis , tumor stages , estrogen or progesterone receptor status . ^^^ CONCLUSION : The expression of VEGF and its receptor FLT 1 , FLK 1 mRNA were up regulated in breast cancer tissues , suggesting its important role in angiogenesis of breast cancer . . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Role of PlGF in the intra and intermolecular cross talk between the VEGF receptors Flt 1 and Flk 1 . ^^^ Here we report that placental growth factor ( PGF , also known as PlGF ) regulates inter and intramolecular cross talk between the VEGF RTKs Flt 1 and Flk 1 . ^^^ Activation of Flt 1 by PGF resulted in intermolecular transphosphorylation of Flk 1 , thereby amplifying VEGF driven angiogenesis through Flk 1 . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
The aim of the present study was to evaluate vascular endothelial growth factor ( VEGF ) , fms like tyrosine kinase 1 ( flt 1 ) , and fetal liver kinase ( flk 1 ) expression in the heart of experimental diabetic rats . ^^^ RT PCR was also performed for VEGF receptors flk 1 and flt 1 . ^^^ No significant difference was found in flt 1 and flk 1 amplification products between STZ induced diabetic rats and controls ( flt 1 / GAPDH densitometric ratio , 0 . 58 + / 0 . 01 5 0 . 64 + / 0 . 05 , P > . 1 ; flk 1 / GAPDH densitometric ratio , 0 . 66 + / 0 . 10 5 0 . 7 + / 0 . 06 , P > . 2 ) . ^^^ The lack of mRNA flt 1 and flk 1 overexpression in diabetic hearts could be one of the mechanisms for this resistance . . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
PTK787 / ZK 222584 ( PTK / ZK ) is an oral potent and selective inhibitor of the vascular endothelial growth factor ( VEGF ) mediated Flt 1 and KDR receptor tyrosine kinases . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Stimulation by either 50 ng / ml VEGF A 165 or by 24 h of anoxia resulted in an altered pattern of receptor expression for VEGF R 2 ( Flk 1 ) , VEGF R 3 ( Flt 4 ) , neuropilin 1 , and neuropilin 2 , whereas VEGF R 1 ( Flt 1 ) remained unexpressed . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
In addition , to confirm extraexpression of the VEGF gene after injection , we assessed the expression of two isoforms of VEGF ( VEGF 120 and VEGF 164 ) in granulosa cells and expression of fms like tyrosine kinase ( Flt 1 ) , expression of fetal liver kinase ( Flk 1 ) , and density of capillary networks in theca cells . ^^^ Granulosa cells and thecal tissues in the antral follicles ( diameter , > 4 mm ) were collected to detect the mRNA expression of VEGF isoforms in the granulosa cells and of Flt 1 and Flk 1 in the thecal tissues by semiquantitative reverse transcription polymerase chain reaction . ^^^ The Flt 1 , but not the Flk 1 , mRNA expression show a tendency toward increasing in the thecal tissues of antral follicles in the ovaries injected with VEGF gene fragments . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Expression of Flt 1 and Flk 1 receptors for vascular endothelial growth factor on tumor cells as a new prognostic criterion for locally advanced breast cancer . ^^^ We studied expression of Flt 1 and Flk 1 receptors on tumor cells obtained from 83 patients with locally advanced breast cancer after neoadjuvant chemotherapy . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Such an autocrine loop is supported by constitutive VEGF receptor ( Flk 1 ) tyrosine phosphorylation , Flk 1 and Flt 1 nuclear localization , and mitogen activated protein kinase activation . beta catenin was also found to exhibit significant nuclear localization and constitutively associate with Flk 1 and Flt 1 in EOMA cells but much less so in HUVEC , and immunoprecipitated Flk 1 was able to phosphorylate purified beta catenin in an immune complex kinase assay . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Distribution of Flk 1 and Flt 1 receptors in neonatal and adult rat brains . ^^^ Double fluorescence staining was combined with confocal laser scanning microscopy to localize fetal liver kinase 1 ( Flk 1 ) and fms like tyrosine kinase 1 ( Flt 1 ) in the neonatal rat brain . ^^^ The results showed that Flk 1 and Flt 1 immunostaining was observed in the cells with neuron specific enolase , a neuronal marker , and with factor 8 ( F 8 ) , an endothelium marker , but not in cells with glial fibrillary acidic protein ( GFAP ) , a glial marker , of brain sections from rats on postnatal day 7 ( P 7 ) . ^^^ A regional analysis showed that Flt 1 was distributed most densely in the hippocampus , followed by the retrosplenial agranular cortex and the striatum , and Flk 1 was evenly distributed throughout the brain . ^^^ In a comparison of the density of immunopositive staining neurons , Flt 1 was much higher than Flk 1 in most of the brain regions . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
The vascular endothelial growth factor ( VEGF ) tyrosine kinase receptors KDR and Flt 1 are targets of current interest in anticancer drug research . ^^^ The development of a hypothesis concerning the bioactive conformation of this compound has led to the discovery of a new class of potent inhibitors of KDR and Flt 1 , the anthranilamides . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
The expression of endothelial cell related markers Flt 1 , Flk 1 , Tie 1 , and Tie 2 was evaluated by RT PCR analysis . beta 2 Adrenergic receptor ( beta 2 AR ) , angiotensin 2 receptor type1A ( AT1A ) , and klotho were also evaluated as markers related to hypertension and multiple sclerosis . ^^^ They also stably expressed Flt 1 and Tie 2 , while Flk 1 , Tie 1 and CXCR 4 were expressed only at low levels in some of the clones . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Expression of the VEGF receptor Flk 1 , but not Flt 1 , was increased in colonic tissue of azoxymethane treated rats compared with control rats . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
We investigated whether acute systemic exercise increases vascular endothelial growth factor ( VEGF ) , VEGF receptor ( KDR and Flt 1 ) mRNA , and VEGF protein in sedentary humans . ^^^ Acute exercise significantly increased VEGF mRNA at 2 and 4 h and increased KDR and Flt 1 mRNA at 4 h postexercise . ^^^ The sustained increase in VEGF mRNA through 4 h and the increases in KDR and Flt 1 at 4 h are different from their respective time course responses in rats . ^^^ These results provide evidence in humans that 1 ) VEGF , KDR , and Flt 1 mRNA are increased by acute systemic exercise ; 2 ) the time course of the VEGF , KDR , and Flt 1 mRNA responses are different from those previously reported in rats ( Gavin TP and Wagner PD . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Vascular endothelial growth factor ( VEGF ) is known to play a predominant role in tumor angiogenesis and metastasis formation that is mediated by its interactions with two tyrosine kinase receptors , VEGFRI ( Flt 1 ) and VEGFRII ( KDR ) . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
We have shown a cyclic regulation of the soluble VEGF receptor , sflt , in human endometrium and have detected the expression of the transmembraneous VEGF receptors , Flt 1 and kinase insert domain containing receptor ( KDR ) throughout the menstrual cycle . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
These include vascular endothelial growth factor , its receptors Flt 1 and Flk 1 , and the angiogenesis associated transcription factor , Ets 1 . . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Using immunohistochemical techniques , we assessed the vascular density and distribution of angiogenesis ( FVIII ) and vascular endothelial growth factor ( VEGF ) expression as well as the expression of 2 VEGF receptors , Flt 1 and Flk 1 , in 55 nonrheumatic and 6 control aortic valves . ^^^ Diseased valves showed distinct VEGF , Flt 1 , Flk 1 , and eNOS positivity of activated endothelial , stromal fusiform myofibroblastic , and histocytic cells . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
In the present study immunohistochemical analysis showed that , in normoperfused mouse hindlimb , VEGF and its receptors Flk 1 and Flt 1 were expressed mostly in quiescent satellite cells . ^^^ At day 3 and day 7 after the induction of ischemia , Flk 1 and Flt 1 were expressed in regenerating muscle fibers and VEGF expression by these fibers was markedly enhanced . ^^^ During myogenic differentiation Flk 1 and Flt 1 were down regulated . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
However , bFGF induced dose dependent phosphorylation of Flt 1 and another VEGF receptor , KDR , in HUVECs but not SMCs . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
We report here that Leydig cell tumours are characterised by an increased level of vascular endothelial growth factor ( VEGF ) in testicular veins , the presence of VEGF mRNA and of its receptor , KDR , and an absence of detectable VEGF receptor Flt 1 , in blood vessels of tumour marginal zones and of peri tumour areas . ^^^ This is in contrast to the capillaries within normal Leydig cell clusters which demonstrate both Flt 1 and KDR . ^^^ These results suggest an autocrine role of VEGF on endothelial cells of tumour blood vessels in a region specific manner and implicate that VEGF interactions with KDR , in the absence of Flt 1 , may be involved in vascular destabilisation . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Vascular endothelial growth factor ( VEGF ) regulates blood vessel formation by binding to the receptor tyrosine kinases VEGF receptor 1 ( Flt 1 ) or VEGF receptor 2 ( KDR ) and to the structurally unrelated neuropilins . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
The mRNA for two VEGF receptors , Flt 1 and Flk 1 , was present in rMVECs and cMVECs . ^^^ Interestingly , rMVECs expressed higher Flt 1 but lower Flk 1 mRNA levels compared to cMVECs . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
GrB / VEGF121 specifically bound ( ELISA ) to porcine aortic endothelial ( PAE ) / FLK 1 cells overexpressing the FLK 1 / KDR receptor but not to cells overexpressing the FLT 1 receptor . ^^^ Immunofluoresence studies showed that the GrB moiety of GrB / VEGF121 was delivered efficiently and rapidly into the cytosol of PAE / FLK 1 cells but not into that of PAE / FLT 1 cells after 4 h treatment with GrB / VEGF121 . ^^^ Treatment of cells with GrB / VEGF121 showed that the IC 50 was approximately 10 nM against PAE / FLK 1 cells ; however , there were no cytotoxic effects observed on PAE / FLT 1 cells at doses up to 200 nM . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
This study compares the mRNA expression of vascular endothelial growth factor ( VEGF ) and its receptors ( KDR and flt 1 ) in the hydrosalpinx with that in the healthy oviduct . ^^^ RESULTS : mRNA expression of VEGF and its receptor flt 1 in the hydrosalpinx was significantly higher than that in the healthy oviduct , but no significant difference was demonstrated for the KDR receptor . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Vascular endothelial growth factor ( VEGF 165 ) exhibits multiple effects via the activation of two distinct endothelial receptor tyrosine kinases : Flt 1 ( fms like tyrosine kinase 1 ) and KDR ( kinase insert domain containing receptor ) . ^^^ KDR shows strong ligand dependent tyrosine phosphorylation in comparison with Flt 1 and mainly mediates the mitogenic , angiogenic , and permeability enhancing effects of VEGF 165 . ^^^ A receptor binding assay revealed that snake venom VEGFs bound to KDR IgG with high affinity ( Kd = approximately 0 . 1 nm ) as well as to VEGF 165 but did not interact with Flt 1 , Flt 4 , or neuropilin 1 at all . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Transcriptional activation by HIF 1 ( receptor flt 1 ) and posttranscriptional mechanism ( receptor KDR ) play a key role in this process . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Northern blot analyses of basic fibroblast growth factor , VEGF , Flt 1 and Flk 1 mRNA expression supported these findings and showed a dose dependent decrease in EtOH treated cultures compared to controls . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
The aim of this study is to determine the localization of VEGF and its receptors , Flt 1 and KDR , and bFGF expression in the rat ovary and to evaluate their distributions throughout the different follicular stages . ^^^ The immunoreactivity for Flt 1 and KDR receptors in growing follicles was mostly limited to GC and TI cells . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Because gene knock outs of VEGF and its receptors flk 1 and flt 1 result in early embryonic lethality , determining roles for VEGF in CNS development has been particularly difficult . ^^^ Antisense oligodeoxynucleotides to flk 1 , but not flt 1 , inhibited neuritic outgrowth , whereas inhibitors of the signaling pathways MEK 1 and P 13 AKT both abrogated VEGF induced growth . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
SUMMARY : The angiogenic role of vascular endothelial growth factor ( VEGF ) receptors , flk 1 and flt 1 , and their downstream signaling pathways , MAPK / ERK and PI 3 kinase , were examined in a fetal rat cortical explant model after exposure to exogenous VEGF . ^^^ Treatment with VEGF resulted in substantial neovascularization characterized by increased vascular flk 1 receptor expression , whereas flt 1 receptor protein expression was absent . ^^^ The specific role of flk 1 receptors in the angiogenic process was confirmed by the addition of antisense oligonucleotides ( AS ODNs ) to flk 1 , which blocked angiogenesis , whereas AS ODNs to flt 1 had no effect . ^^^ These results were further supported by the finding that specific chemical inhibition of flk 1 receptors caused disruption of the angiogenic response , whereas inhibition of the flt 1 receptors had no effect . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
MATERIALS AND METHODS : Using RT PCR we examined the expression of VEGF and its receptors , KDR , Flt 1 , NP 1 and NP 2 , in 8 human prostate cancer cell lines and then correlated the expression patterns with the ability of these cell lines to invade and migrate through membranes in a modified Boyden Chamber assay in the presence and absence of VEGF . ^^^ None expressed the Flt 1 receptor and only 2 expressed KDR . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Correlation between transcriptional activity of vascular endothelial growth factor ( VEFG ) and transcriptional activity of its receptors ( FLT 1 and FLK 1 ) in low grade squamous intraepithelial lesions ( LSIL ) of uterine cervix ] . ^^^ VEGF , Flt 1 , Flk 1 . ^^^ To estimate the dependence transcriptional activity of Flt 1 and Flk 1 on VEGF gene expression Spearman ' s correlation rank was performed . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Late EPC was different from early EPC in the expression of VE cadherin , Flt 1 , KDR , and CD 45 . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Expression of VEGF receptors ( flt 1 , flk 1 ) , endoglin and hypoxia inducible factor 1alpha ( HIF 1alpha ) was assessed by quantitative RT PCR and for endoglin also by immunohistochemistry . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Primary varicose veins : altered transcription of VEGF and its receptors ( KDR , flt 1 , soluble flt 1 ) with sapheno femoral junction incompetence . ^^^ Our aim was to examine transcription of genes for VEGF ( VEGF ( 121 ) / VEGF ( 165 ) ) and its receptors ( KDR , flt 1 , s . flt 1 ) in VVs , in relation to underlying venous incompetence . ^^^ RESULTS : VEGF ( 121 ) / ( 165 ) , KDR and flt 1 transcription was elevated in VVs overall ( p < 0 . 001 ) , and in VVs with an incompetent SFJ ( p < 0 . 001 ) , but not when the SFJ was functional ; s . flt 1 was unaltered . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
We investigated the genetic basis of VEGF expression in patients with type 1 ( onset before age 40 y ) chronic plaque psoriasis compared to healthy controls and also measured plasma levels of VEGF and its receptors flt 1 and KDR . ^^^ Plasma levels of VEGF and flt 1 were significantly detectable in patients with psoriasis compared with controls ( p < 0 . 001 ) ; by contrast , mean plasma levels of KDR in psoriatic patients were comparable with controls . ^^^ VEGF , flt 1 , and KDR could provide attractive targets for future psoriasis therapy . . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
In vivo regulation of mRNA expression of vascular endothelial growth factor receptors ( KDR and flt 1 ) in the human oviduct . ^^^ RESULT ( S ) : Immunohistochemical study localized VEGF R , both KDR and flt 1 , in the oviduct luminal epithelium , smooth muscle cells as well as blood vessels within the oviduct . ^^^ Messenger RNA expression of KDR , but not flt 1 , was significantly higher in the ampullary and infundibular regions than in the isthmus . ^^^ Messenger RNA expression of flt 1 , but not KDR , varied significantly in the oviduct along the course of an ovulatory cycle , with the highest level in the periovulatory stage . ^^^ Our data support a role for KDR in oviduct angiogenesis whereas flt 1 appears to be important in the temporal regulation of oviductal secretion . . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Sections were stained with antibodies against CD 31 , Flt 1 , KDR , endothelin 1 ( ET 1 ) and endothelin receptor A ( ET ( A ) ) . ^^^ We also performed immunohistochemical staining for the VEGF receptors Flt 1 and KDR . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Increased expression of VEGF receptors ( FLT 1 , KDR , NRP 1 ) and thrombospondin 1 is associated with glomeruloid microvascular proliferation , an aggressive angiogenic phenotype , in malignant melanoma . ^^^ Presence of GMP was associated with increased expression in tumour endothelium of the VEGF A receptors KDR , FLT 1 and neuropilin 1 , as well as VEGF D protein . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Interestingly , levels of VEGF , VEGF receptors ( KDR , Flt 1 ) , endothelial nitric oxide synthase , phosphorylated Akt ( pAkt ) , estrogen receptor alpha , aromatase , and capillary density in sham operated SHRSP were remarkably down regulated , whereas androgen receptor levels were up regulated , compared with age matched genetic control , Wistar Kyoto rats . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Furthermore , using mouse wound healing models , we demonstrated that adenovirus mediated delivery of EPAS 1 gene significantly induced the expression of VEGF , Flt 1 , Flk 1 , and Tie 2 mRNA at the wound site and promoted mature angiogenesis . ^^^ In conclusion , EPAS 1 promotes Flt 1 gene expression and induces mRNA expression of VEGF , Flk 1 , and Tie 2 , leading to enhancement of mature angiogenesis in vivo . ^^^ Thus , EPAS 1 may contribute to the construction of mature vessels by modulating the coordinated expressions of VEGF , Flt 1 , Flk 1 , and Tie2 . . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
These mouse endothelial cell antigen , endothelial nitric oxide synthase ( eNOS ) , Flk 1 , Flt 1 , and CD 31 positive cells engrafted into renal microvasculature and significantly protected short term renal function after ischemia . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
In this study , the expression of vascular endothelial growth factor ( VEGF ) , basic fibroblast growth factor ( bFGF ) , and VEGF receptors , fms like tyrosine kinase ( Flt 1 ) and kinase insert domain containing region ( KDR ) , and bFGF receptor 1 ( Flg ) were examined in the endometrium of rhesus monkey on Day 5 , 10 , 16 , 20 , 25 of menstrual cycle and on Day 19 of early pregnancy . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
We used old C57BL / 6J mice that are affected by rapid and severe age related hearing loss , and analyzed the expression pattern of vascular endothelial growth factor ( VEGF ) , a prototypical angiogenic cytokine , and its receptors Flt 1 and Flk 1 in the inner ear . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
To investigate whether VEGF and VEGF receptor [ kinase insert domain containing receptor ( KDR ) and Flt 1 ] expression are lower with obesity , vastus lateralis muscle biopsies were obtained from eight obese and eight lean young sedentary men before and 2 h after a 1 h submaximal aerobic exercise bout for the measurement of VEGF , KDR , Flt 1 , and skeletal muscle fiber and capillary characteristics . ^^^ Exercise increased VEGF ( 10 fold ) , KDR ( 3 fold ) , and Flt 1 ( 5 fold ) mRNA independent of group . ^^^ There were no differences in VEGF , KDR , or Flt 1 protein between groups . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
During the first week of treatment , vascular endothelial growth factor ( VEGF ) , VEGF receptor 1 ( Flt 1 ) , and basic fibroblast growth factor proteins were higher in the treated group , whereas VEGF receptor 2 ( Flk 1 ) , angiopoietin 1 , and angiopoietin 2 were not affected by treatment . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
OBJECTIVE : To explore the expression of vascular endothelial growth factor ( VEGF ) and its receptors ( flt 1 and flk 1 ) in the retina of retinopathy of prematurity ( ROP ) , and its relation to the alteration of retinal blood vessels . ^^^ The expression of VEGF , flt 1 and flk 1 in the retina was measured by immunohistochemistry . ^^^ However , flt 1 has less effect compared with flk 1 . . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Biological effects of VEGF are mediated by endothelial cell surface receptors , KDR and Flt 1 . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
OBJECTIVE : To compare the mRNA expression of vascular endothelial growth factor ( VEGF ) and its receptors ( KDR and flt 1 ) in the implantation and nonimplantation sites of the human oviduct with ectopic gestation . ^^^ RESULT ( S ) : The mRNA expression of VEGF and its receptors , both KDR and flt 1 , was significantly higher in the implantation site of the human oviduct with ectopic gestation compared with the nonimplantation site . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
VEGF receptor Flt 1 and Flk 1 play key roles in angiogenesis . ^^^ The Flt 1 and tyrosine hydroxylase proteins were significantly higher , and Flk 1 significantly lower in placentae from preeclamptic compared to normal pregnancies . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
The sFlt 1 gene transfer did not affect plasma lipid levels but attenuated increased expression of VEGF , Flt 1 , Flk 1 , monocyte chemoattractant protein 1 , and other inflammation promoting factors . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
METHODS AND RESULTS : We quantitated the mRNA expression of EPC specific molecules ( eg , Flk 1 , Flt 1 , CD 133 , VE cadherin , etc ) in bone marrow derived or peripheral blood derived mononuclear cells obtained from patients with ischemic limbs , using real time reverse transcription polymerase chain reaction technique . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Expression of vascular endothelial growth factor and its receptors Flt 1 and Flk 1 was studied in lumbar spinal cord after sciatic nerve crush injury . ^^^ Immunohistochemical staining revealed strikingly different distribution of VEGF , Flt 1 , and Flk 1 in lumbar motor neurons . ^^^ VEGF was observed both in the nuclei and perikarya , while Flk 1 had cytoplasmic and Flt 1 perinuclear localization . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Vascular endothelial growth factor induced nitric oxide and PGI 2 dependent relaxation in human internal mammary arteries : a comparative study with KDR and Flt 1 selective mutants . ^^^ The role of the vascular endothelial growth factors ( VEGF ) receptors ( KDR and Flt 1 ) and their characteristics in VEGF induced vasodilation in human vessels is unclear . ^^^ This study investigated the in vitro vasorelaxant effects of KDR selective ( KDR SM ) and Flt 1 selective mutants ( Flt 1 SM ) in the human internal mammary artery ( IMA ) . ^^^ The cumulative concentration ( 12 to 8 log10M ) relaxation curves were established for VEGF , KDR SM , Flt 1 SM , and placenta growth factor ( PlGF ) in the absence or presence of indomethacin ( INDO , 7 microM ) , N nitro L arginine ( L NNA , 300 microM ) , L NNA + oxyhemoglobin ( HbO , 20 microM ) , or INDO + L NNA + HbO . ^^^ The maximal relaxation induced by KDR SM ( 53 . 0 + / 4 . 0 % ) was only slightly less than that by VEGF ( P = 0 . 075 ) but significantly more than that by Flt 1 SM ( P = 0 . 001 , 95 % CI 7 . 8 41 . 1 % ) . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Together with flt 1 ( VEGF receptor 1 ) and flk 1 ( VEGF receptor 2 ) , neuropilin ( NP ) is frequently described as being involved in the neuroprotective effects of VEGF . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Vascular endothelial growth factor A ( VEGF A ( 165 ) ) exerts multiple effects upon binding to the fms like tyrosine kinase 1 ( Flt 1 ) and the kinase insert domain containing receptor ( KDR ) . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
STUDY DESIGN : Messenger RNA levels for vascular endothelial growth factor , flt 1 , and KDR were measured by TaqMan real time polymerase chain reaction in human placental choriocarcinoma cells ( BeWo ) that were exposed to hypoxia ( 1 % oxygen , 5 % carbon dioxide , 94 % nitrogen ) and in placental tissue of neonates with birth asphyxia ( n = 20 ) , newborn infants of mothers with preeclampsia ( n = 20 ) , and gestational age matched control subjects . ^^^ RESULTS : In BeWo cells , vascular endothelial growth factor , flt 1 and KDR messenger RNA increased in a time dependent manner in response to hypoxia . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
METHODS : Colonic specimens were taken from 42 patients with ulcerative colitis ( UC ) , 37 with Crohn ' s disease ( CD ) , 8 with non IBD colitis , and 21 normal controls . ( 1 ) Expression of vascular endothelial growth factor ( VEGF ) , VEGF receptors ( Flt 1 , KDR ) , and Ets 1 proteins in colonic mucosa was immunohistochemically examined using specific antibodies . ( 2 ) Expression of Ets 1 protein or VEGF , Flt 1 , KDR , and Ets 1 mRNA in colonic mucosa was measured by Western blot or RT PCR . ^^^ The numbers of positive cells to these antibodies were also increased in active UC . ( 2 ) Expression of Ets 1 protein and Flt 1 , KDR , and Ets 1 mRNA was increased in active UC . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
This study was designed to test the hypothesis that ( a ) the cervix of pregnant rats expresses vascular endothelial growth factor ( VEGF ) and components of the angiogenic signaling pathway [ VEGF receptors ( Flt 1 , KDR ) , activity of protein kinase B , Akt ( phosphorylated Akt ) , and endothelial nitric oxide synthase ( eNOS ) ] and von Willebrand Factor ( vWF ) and that these molecules undergo changes with pregnancy , and ( b ) bilateral pelvic neurectomy ( BLPN ) alters levels of VEGF concentration in the cervix . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
RESULTS : In 107 AML patients , the positive mRNA expression rate of VEGF , KDR and Flt 1 was 56 . 1 % , 49 . 5 % , and 2 . 8 % , respectively ; for bFGF and FGFR 1 4 , the positive rate was 21 . 5 % , 35 . 5 % , 9 . 4 % , 35 . 5 % and 23 . 4 % , respectively . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Shear stress significantly increased the expression of the vascular endothelial cell specific markers Flk 1 , Flt 1 , vascular endothelial cadherin , and PECAM 1 at both the protein level and the mRNA level , but it had no effect on expression of the mural cell marker smooth muscle alpha actin , blood cell marker CD 3 , or the epithelial cell marker keratin . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
METHODS : Ovine left ( LV ) and right ventricle ( RV ) samples were obtained at four time points : 95 days ' and 140 days ' gestation ( term = 145 days ) and 7 days and 8 weeks postnatally . mRNA and protein levels of vascular endothelial growth factor ( VEGF ) , its respective receptors ( Flk 1 and Flt 1 ) , basic fibroblast growth factor ( bFGF ) , transforming growth factor beta 1 ( TGF beta 1 ) , and endothelial nitric oxide synthase ( eNOS ) were measured at these different time points . ^^^ CONCLUSION : Developmentally regulated ventricle specific expression of VEGF , Flt 1 , Flk 1 , TGF beta 1 , bFGF , and eNOS was demonstrated in the ovine myocardium . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
EXPERIMENTAL DESIGN : Real time quantitative reverse transcriptase PCR was used to measure levels of mRNA of tumor angiogenesis and lymphangiogenesis related factors [ vascular endothelial growth factor ( VEGF ) A , VEGF C , VEGF D , Flt 1 , KDR , Flt 4 , Ang 1 , Ang 2 , Tie 1 , Tie 2 , cyclooxygenase 2 , fibroblast growth factor 2 ( FGF 2 ) , Egr 1 , Prox 1 , and LYVE 1 ] in tumor specimens of 16 inflammatory breast cancer and 20 noninflammatory breast cancer patients . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
As analyzed by the semiquantitative reverse transcriptase polymerase chain reaction , hCG treatment significantly increased mRNA expression of vascular endothelial growth factor and its receptor flt 1 in the cultured mucosal cells in a dose dependent manner but had no effect on the expression of another receptor , KDR . . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Urinary gonadotrophins but not recombinant gonadotrophins reduce expression of VEGF 120 and its receptors flt 1 and flk 1 in the mouse uterus during the peri implantation period . ^^^ RESULTS : Urinary gonadotrophin treatment caused lower levels of VEGF 120 , flt 1 and flk 1 mRNA levels , reduced the size of the embryo implantation site , delayed implantation and prolonged the gestational period . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Immunohistochemical staining for vascular endothelial growth factor ( VEGF ) , its receptors Flk 1 and Flt 1 , ephrin B 2 , MIB 1 and proliferating cell nuclear antigen ( PCNA ) was performed using standard immunohistochemical techniques . ^^^ Flk 1 showed a positive immunoreaction in only 2 / 6 cases and Flt 1 in 4 / 6 cases . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
This finding is consistent with the altered expression of vegf , flt 1 , kdr , and tie 1 in the mutant embryos . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
ROP eyes were further analyzed for changes in VEGF and VEGF receptor ( Flt 1 and Flk 1 ) protein content following rapamycin treatment . ^^^ Immunostaining for Flt 1 increased , while no obvious changes in Flk 1 were observed . ^^^ Quantitative analysis of total protein via enzyme linked immunosorbent assay ( ELISA ) confirmed an increase in Flt 1 and VEGF , following drug treatment , with no effect on Flk 1 . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
METHODS : Bone marrow biopsies from 122 cases with different stages of human acute leukemia were immunostained with anti vascular endothelial growth factor , anti fms like tyrosine kinase ( Flt 1 ) and anti kinase domain insert containing receptor ( KDR ) antibodies with envision two step immunohistochemical staining method . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Vascular endothelial growth factor mediated cell survival signalling occurs via the classical tyrosine kinase receptors Flt 1 , KDR / Flk 1 and the more novel neuropilin ( NP ) receptors , NP 1 and NP 2 . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
We examined the radiological and histological features of , and the influences of the expression of VEGF and its two major receptors , Flt 1 and Flk 1 , on the development of peritumoral brain edema ( PTBE ) in patients with intracranial meningiomas . ^^^ The positivity of Flt 1 and Flk 1 was mainly observed in tumor vessels ; 44 cases ( 37 . 2 % ) were positive for the Flt 1 antibody and 37 cases ( 31 . 4 % ) for the Flk 1 antibody . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Finally , we showed that VEGF induced TAL 1 expression in the NGM which resulted in the formation of ectopic structures was mediated by Flk 1 but not Flt 1 signaling . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Effect of Weikangning on gastric cancer cell growth and expression of vascular endothelial growth factor and its receptors KDR and Flt 1 . ^^^ AIM : To observe the effect of Chinese traditional herbal decoction Weikang ning ( WKN ) on cell growth and expression of VEGF and its receptors KDR and Flt 1 in gastric cancer cell line MGC 803 . ^^^ The expression of mRNA of VEGF and its receptors KDR and Flt 1 was detected with RT PCR . ^^^ CONCLUSION : The decoction of WKN suppresses the growth of gastric cancer cell MGC 803 and decreases the expression of mRNA of both VEGF and its receptors KDR and Flt 1 . . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
The non angiogenic role of vascular endothelial growth factor ( VEGF ) , and its receptors flt 1 and flk 1 , together with downstream signaling pathways were examined in fetal and postnatal rat cerebral cortical organotypic explants . ^^^ The VEGF receptor flt 1 was observed on most , though not all astrocytes , while flk 1 receptor immunoexpression was absent . ^^^ Treatment with antisense oligonucleotides ( AS ODNs ) to flt 1 resulted in a dramatic decrease in GFAP and nestin immunoreactivity , which further confirmed the role of flt 1 in mediating VEGF ' s gliotrophic effects , while AS ODNs to flk 1 had no effect . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Flt 1 and Flk 1 receptors were highly expressed by endothelial cells from medium and small vessels in HP tissue . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
The inhibition of FGFR 3 expression by ribozymes was associated with decreased vascular endothelial growth factor ( VEGF ) expression and upregulation of Flt 1 but not of the KDR receptor . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Our data lend support to the hypothesis that VEGF is essential for pregnancy establishment and that trophoblast derived VEGF , acting via its specific receptors Flt 1 and KDR , is necessary for blastocyst implantation . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
By immunohistochemical staining , VEGF was not detected in normal uterine smooth muscle , but VEGF receptor 1 ( flt 1 ) and VEGF receptor 2 ( flk 1 ) were observed in 14 and 4 of 14 normal smooth muscles , respectively . ^^^ Of 39 sarcomas , 25 expressed VEGF , and 38 and 34 sarcomas expressed flt 1 and flk 1 at various intensities , respectively . ^^^ The staining intensity of VEGF , flt 1 , and flk 1 was significantly higher in sarcoma than in normal uterine smooth muscle , but that of phospho flt 1 ( p flt 1 ) was significantly lower in sarcoma than in normal uterine smooth muscle . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
We studied protein expression of basic fibroblast growth factor ( bFGF ) , vascular endothelial growth factor ( VEGF ) A , endothelin ( ET ) 1 , and VEGF C , and their receptors bFGF R 1 , Flt 1 , KDR , ET ( A ) R , ET ( B ) R , and Flt 4 immunohistochemically in 200 DCIS ( pure DCIS : n=96 ; DCIS adjacent to an invasive component : n=104 ) using self constructed tissue microarrays . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Surgical specimens and tumor cells in primary culture of salivary pleomorphic adenomas were used for immunohistochemistry for CD 31 , vascular endothelial growth factor ( VEGF ) and its receptors Flk 1 and Flt 1 , as well as for hypoxia markers , such as hypoxia inducible factor 1alpha ( HIF 1alpha ) and lactate dehydrogenase 1 ( LDH ) . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
We used reverse transcriptase PCR to measure mRNA expression for VEGF A isoforms ( VEGF A ( 120 ) , ( 144 ) , ( 164 ) , and ( 188 ) ) and VEGF A receptors , Flt 1 and Flk 1 . ^^^ With advancing development , mRNA content increased only for VEGF A ( 188 ) ( p < 0 . 05 ) and for Flt 1 ( p < 0 . 02 ) and Flk 1 ( p < 0 . 005 ) . ^^^ Neither TO nor CDH consistently affected Flt 1 or Flk 1 mRNA content . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
We evaluated the effect of transplanted cell type , time , and region of the heart on expression of the vascular endothelial growth factor receptors fms like tyrosine kinase 1 ( flt 1 ) and fetal liver kinase 1 ( flk 1 ) . ^^^ Flt 1 and flk 1 expression in the scar , border zone , and normal myocardium were evaluated at 3 days and 1 , 2 , and 4 weeks by quantitative polymerase chain reaction . ^^^ Transplanted cells , vascular endothelial growth factor , flt 1 , and flk 1 were identified by immunohistology . ^^^ RESULTS : Flt 1 and flk 1 levels were low in all areas of control hearts . ^^^ Upregulation of flt 1 and flk 1 after cell transplantation occurred primarily in host cells in the border zone rather than the scar ( zone , p < 0 . 0001 ) . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Effect of rhodiola on expressions of Flt 1 , KDR and Tie 2 in rats with ischemic myocardium ] . ^^^ Flt 1 , KDR and angiopoietin receptor ( Tie 2 ) were measured . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Vascular endothelial growth factor exerts multiple functions through binding to two distinct receptor tyrosine kinases , Flt 1 and KDR . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Furthermore , VEGF receptor fms like tyrosine kinase 1 ( Flt 1 ) was elevated , whereas kinase insert domain containing receptor / fetal liver kinase 1 ( KDR ) was diminished in endothelial , but not epithelial , cells . ^^^ Intracellular Flt 1 and KDR locations were unchanged by PHD blockade . ^^^ Like VEGF , FG 4095 and DMOG increased angiogenesis in vitro , both in 95 % and 21 % O 2 , an effect that could be blocked through either Flt 1 or KDR . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
At the early stage ( day 6 ) , glomerular expression of VEGF and receptors flk 1 and flt 1 as well as Ang 1 , and receptor Tie 2 were increased , and glomerular monocyte infiltration and the expression of angiopoietin 2 ( Ang 2 ) , a natural antagonist of Ang 1 , were reduced . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
KDR bp also binds to a lesser extent with Flt 1 and IgG but not to other receptors with similar immunoglobulin like domain structures such as platelet derived growth factor receptor alpha . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Soluble vascular endothelial growth factor receptors 1 , 2 ( Flt 1 , KDR ) are the negative counterpoint to the vascular endothelial growth factor ( VEGF ) signaling pathway , which has been characterized as one of the most important endothelial regulator in human angiogenesis . ^^^ In the present work , we tested the differential prognostic relevance of soluble vascular endothelial growth factor ( VEGF ) , their receptors 1 ( Flt 1 ) , 2 ( KDR ) , and the ratio between sVEGF / sFlt 1 in 43 patients with acute myeloid leukemia ( AML ) . sVEGF and its soluble receptors were assessed using an ELISA . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
RESULTS : There were no significant differences in the mRNA expression of VEGF and its receptors , both KDR and flt 1 , between the five study groups and the control group . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Semi quantitative RT PCR analysis showed that VEGF receptor Flk 1 mRNA was also time dependently upregulated in the deafferented hippocampus with its maximal elevation at 7 15 days postlesion while the Flt 1 mRNA levels remained unchanged at any time point we examined . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
However , transcripts for VEGF 189 , VEGF 165 , and VEGF receptors ( Fms like tyrosine kinase 1 [ Flt 1 ] , kinase insert domain receptor [ KDR ] / fetal liver kinase 1 [ Flk 1 ] , and neuropilin 1 ) were suppressed in the BPD models . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Bioactivity of TNF in tissues was confirmed by overexpression of Icam 1 , Sele , Vegfa , Flt 1 and Kdr . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Biochemical studies revealed that TKI 28 potently inhibited the activities of tyrosine kinases such as ErbB 2 , EGFR , KDR , PDGFRbeta , c kit and c Src , but had little effect on Flt 1 in cell free system . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
SK N BE 2 , a highly malignant neuroblastoma cell line resistant to hypoxia induced apoptosis expresses active Flt 1 but lacks VEGFR 2 expression . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Vascular endothelial growth factor and VEGF receptors ( flt 1 and flk 1 ) were evaluated by Western blot , reverse transcriptase polymerase chain reaction ( RT PCR ) and real time RT PCR . ^^^ RESULTS : Vascular endothelial growth factor , flt 1 , and flk 1 messenger ribonucleic acid ( mRNA ) and protein expressions were increased in non diabetic patients with CHD compared with control subjects . ^^^ Remarkably , in diabetic patients , VEGF mRNA and protein levels were significantly higher , whereas flt 1 , flk 1 mRNA , and protein were lower when compared with non diabetic patients . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Treatment with vascular endothelial cell growth factor ( VEGF ) gave rise to endothelial like cells , expressing Flt 1 , Flk 1 , von Willebrand Factor ( vWF ) , CD 31 , acetylated low density lipoprotein internalization , and the ability to form endothelial like cell chains . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
However , only the combination of VEGF with LH , E 2 , and P 4 upregulated mRNA for Ace 1 and Ang 2 release , but not VEGF alone . ( 2 ) Treatments of LH , with E 2 and / or P 4 increased the mRNA expression for VEGF , Flk 1 and Flt 1 , and ( 3 ) VEGF itself downregulated the expression of mRNA for VEGF , and LH , E 2 , and P 4 enhanced this downregulatory effect . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
BACKGROUND : Vascular endothelial growth factor ( VEGF ) and its tyrosine kinase family of receptors ( VEGFR ) ( Flt 1 , Flk 1 , Flt 4 ) have been implicated in vascular angiogenesis and remodelling in cerebral arteriovenous malformations ( CAVM ) . ^^^ METHODS : Surgical specimens from 26 patients undergoing CAVM resection were studied for HIF 1alpha , VEGF , Flt 1 , Flk 1 and Flt 4 . ^^^ HIF 1alpha expression was significantly associated with VEGF , Flt 1 and Flk 1 expression ( p < 0 . 05 ) CONCLUSIONS : HIF 1alpha is expressed in human CAVM . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Skeletal muscle biopsies were obtained from the vastus lateralis before and at 4 h after a submaximal exercise bout for the measurement of morphometry , capillarization , VEGF , KDR , and Flt 1 in seven aged ( mean age 65 yr ) and eight young ( mean age 21 yr ) sedentary men . ^^^ There were no effects of exercise or age on muscle Flt 1 mRNA or protein or KDR protein . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
OBJECTIVE : To study the localization and expression of the vascular endothelial growth factor receptors ( VEGFR ) Fms like tyrosine kinase ( Flt 1 ) and kinase insert domain containing receptor ( KDR ) in human ectopic and eutopic endometrium of patients with endometriosis . ^^^ Immunohistochemistry was used to detect the location and expression of Flt 1 and KDR protein in different tissues . ^^^ Western blotting was used to detect the protein expression of Flt 1 and KDR protein in different tissues . ^^^ The mRNA expressions of Flt 1 and KDR were detected by RT PCR . ^^^ RESULTS : Flt 1 and KDR were expressed in the endometrial glandular epithelium and stromal cells besides the endometrial blood vessels . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Immunohistochemical staining for vascular endothelial growth factor ( VEGF ) , its receptors Flk 1 and Flt 1 , transforming growth factor alpha ( TGFalpha ) , basic fibroblast growth factor ( bFGF ) , hypoxia inducible factor 1alpha ( Hif 1alpha ) , MIB 1 and proliferating cell nuclear antigen ( PCNA ) was performed using standard immunohistochemical techniques . ^^^ Flk 1 showed a positive immunoreaction in only 2 / 8 cases and Flt 1 in 5 / 7 cases . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Moreover , VEGF receptors KDR and / or Flt 1 were decreased in BPD . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Vascular endothelial growth factor ( VEGF ) and its receptors ( FLT 1 and KDR ) are overexpressed by human bladder cancer cells and tumor endothelial cells , respectively . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
The mRNA expressions of VEGF and its receptors Flt 1 and Flk 1 were evaluated by semi quantitative reverse transcription PCR . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
RESULTS : Before induction , MS 7 cells expressed mRNA of fetal liver kinase 1 ( Flk 1 ) , fms like tyrosine kinase ( Flt 1 ) , tyrosine kinase with Ig and EGF homology domains 2 ( Tie 2 ) , CD 31 , and podocalyxin . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Prostate cancer cells expressed the mRNA for VEGF receptor ( VEGFR ) neuropilin 1 but not the VEGFRs Flt 1 or KDR . ^^^ In contrast , mouse pre osteoblastic cells ( MC3T3 E 1 ) expressed Flt 1 and neuropilin 1 mRNA but not KDR . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Interestingly , GDMEC expressed higher levels of VEGF receptors , flt 1 and flk 1 , as compared to an established human EC cell line ECV 304 and primary human umbilical vascular EC ( HUVEC ) . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Serum levels of vascular endothelial growth factor ( VEGF ) , soluble Flt 1 and Flk 1 receptors , placental growth factor ( PlGF ) , angiopoietin 2 ( Ang 2 ) and soluble Tie 2 receptor were determined by ELISA . ^^^ Interestingly , in CHC patients serum levels of VEGF and Tie 2 correlated with grade of inflammation , PlGF correlated with stage of fibrosis , and Flt 1 and Flk 1 correlated with serum transaminase levels ( p < 0 . 05 in all cases ) . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
OBJECTIVE : In order to clarify the role of vascular growth factor in the modulation of the vascular network of the cochlea , we studied the expression of VEGF and its receptors fms like tyrosine kinase ( Flt 1 ) and foetal liver kinase ( Flk 1 ) in the inner ear of 3 month old rodents of different species : C57BL / 6J mice , Wistar albino rats and Hartley albino guinea pigs . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
In contrast , gene transfer of placental growth factor ( PGF ) which signals through Flt 1 , but not KDR receptors had negative effects on neurogenesis and inhibited learning , although it similarly increased endothelial cell proliferation . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Expression and clinical significance of VEGF and its receptors Flt 1 and KDR in nasopharyngeal carcinoma ] . ^^^ BACKGROUND & OBJECTIVE : Vascular endothelial growth factor ( VEGF ) and its receptors fms like tyrosine kinase 1 ( Flt 1 ) and kinase insert domain containing receptor ( KDR ) play critical roles in angiogenesis and tumor progression . ^^^ This study was to explore the correlations of VEGF , Flt 1 and KDR expression to clinical features and prognosis of nasopharyngeal carcinoma ( NPC ) patients . ^^^ METHODS : Immunohistochemistry was adopted to detect the expression of VEGF , Flt 1 , and KDR in 127 specimens of NPC . ^^^ RESULTS : The positive rates of VEGF , Flt 1 , and KDR were 66 . 9 % , 90 . 6 % , and 88 . 2 % in the NPC specimens . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
We also report on the existence of likely dominant negative forms for many of the receptor kinases and phosphatases , including some 26 secreted decoys ( seven known and 19 novel : Alk , Csf1r , Egfr , Epha 1 , 3 , 5 , 7 and 10 , Ephb 1 , Flt 1 , Flt 3 , Insr , Insrr , Kdr , Met , Ptk 7 , Ptprc , Ptprd , Ptprg , Ptprl , Ptprn , Ptprn 2 , Ptpro , Ptprr , Ptprs , and Ptprz 1 ) and 13 transmembrane forms ( four known and nine novel : Axl , Bmpr1a , Csf1r , Epha 4 , 5 , 6 and 7 , Ntrk 2 , Ntrk 3 , Pdgfra , Ptprk , Ptprm , Ptpru ) . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Vascular endothelial growth factor , FLT 1 , and FLK 1 analysis in a pancreatic cancer tissue microarray . ^^^ The objective of this study was to use a series of algorithms called AQUA that quantitatively assesses protein expression on tissue microarrays ( TMAs ) to compare in situ expression of VEGF and its primary receptors , VEGF receptor 1 ( FLT 1 ) and VEGF receptor 1 ( FLK 1 ) , on a pancreatic cancer TMA . ^^^ The primary antibodies used were VEGF , FLT 1 , FLK 1 , and cytokeratin . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Rats underwent RT PCR determination of one of the VEGF receptors flt 1mRNA , and immunohistochemistry for VEGF receptors Flt 1 and Flk 1 . ^^^ No Flt 1 and Flk 1 positive cell was observed in the brain in non SAH group . ^^^ A good few Flt 1 and Flk 1 positive cells were found in cortex and other regions of the brain in SAH group . ^^^ The expression of flt 1mRNA , Flt 1 and Flk 1 proteins were increased by the use of two doses of EGb . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Five micrometers sections were submitted to standard immunohistochemistry protocol using three primary antibodies ( SC 315 , SC 316 and VG76e ) for detection of kinase domain region ( KDR ) , fms like tyrosine kinase 1 ( Flt 1 ) and VEGF , respectively . ^^^ We concluded that VEGF might have a modulatory effect in the CL of the dog acting as paracrine and autocrine factor through its receptors , Flt 1 and KDR . . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Our study analyzed the expression of Vascular Endothelial Growth Factor ( VEGF ) and its functional receptors , Flt 1 and Flk 1 , in the cochlear structures of noise exposed and unexposed guinea pigs . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
The mRNA and protein expressions of VEGF and of fms like tyrosine kinase 1 ( Flt 1 ) and fetal liver kinase 1 ( Flk 1 ) , which are main VEGF receptors , in the heart were significantly lower in the sedentary aged rats compared with the sedentary young rats , whereas those in the exercise trained rats were significantly higher than those in the sedentary aged rats . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
In addition to VEGF A , the cells expressed mRNAs for various members of the VEGF family and for their receptors , including VEGF B , C , D , flt 1 , and KDR . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Kdr mRNA was present on E13 . 5 , whereas Fms like tyrosine kinase 1 receptor ( Flt 1 ) mRNA was not detected until E 18 . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Study of microvessel density and the expression of the angiogenic factors VEGF , bFGF and the receptors Flt 1 and FLK 1 in benign , premalignant and malignant prostate tissues . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Prognostic value of expression of vascular endothelial growth factor and its flt 1 and KDR receptors in stage 1 non small cell lung cancer . ^^^ PATIENTS AND METHODS : We examined the prognostic value of the expression of vascular endothelial growth factor ( VEGF ) and of the VEGF receptors ( VEGFRs ) fms like tyrosine kinase receptor 1 ( flt 1 ) and kinase insert domain containing receptor ( KDR ) in non small cell lung cancer ( NSCLC ) . ^^^ Multivariate analysis identified expression of both flt 1 and KDR and VEGF and KDR as possible independent prognostic factors . ^^^ Moreover , tumors expressing both flt 1 and KDR may have greater malignant potential and are associated with a poor prognosis . . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
We found that VEGF receptor FMS like tyrosine kinase 1 ( Flt 1 ) and fetal liver kinase 1 ( Flk 1 ) expression increased during ESC differentiation . ^^^ Antibodies against Flk 1 totally blocked and against Flt 1 partially blocked VEGF induced NKx2 . 5 positive stained cells . ^^^ Our results suggest that VEGF promotes cardiomyocyte differentiation predominantly by ERK mediated Flk 1 activation and , to a lesser extent , by Flt 1 activation . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Systemic administration of antibodies against the transmembrane receptor Flk 1 but not Flt 1 protected against sepsis mortality . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Using samples of CCE and COE , we immunohistochemically determined protein expression of the angiogenesis related factors : Thrombospondin 1 ( TSP 1 ) , pigment epithelium derived factor ( PEDF ) , endostatin , angiostatin , vascular endothelial growth factor ( VEGF ) , Fms like tyrosine kinase 1 ( Flt 1 ) , kinase insert domain receptor ( KDR ) , and basic fibroblast growth factor ( bFGF ) . ^^^ Immunohistochemically there was no obvious difference between CCE and COE with respect to angiostatin , VEGF , Flt 1 , KDR , and bFGF . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Vascular endothelial growth factor A ( VEGF ) , which binds to both VEGF receptor 1 ( Flt 1 ) and VEGFR 2 ( KDR / Flk 1 ) , requires nitric oxide ( NO ) to induce angiogenesis in a cGMP dependent manner . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
The body weight was decreased in the diabetic rats compared with the control rats , but the left ventricular ( LV ) body weight ratio was increased in the diabetic group and was unaffected by treatment with ET antagonist . mRNA expression of VEGF and its receptors ( Flt 1 and Flk 1 ) in the LV tissues was assessed using real time polymerase chain reaction . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Reverse transcriptase polymerase chain reaction analyses demonstrated that the expression of PECAM , basic fibroblast growth factor ( bFGF ) , and smooth muscle 22 alpha ( SM22alpha ) was upregulated in association with the tube formation , whereas the expression of Flk 1 , Flt 1 , and hepatocyte growth factor ( HGF ) was gradually downregulated . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
The increase of flk 1 and flt 1 ( VEGF receptors ) and tie 2 ( Ang 1 receptor ) induced by ang 2 was significantly suppressed by PD 123319 . ^^^ The increase of VEGF / flk 1 / flt 1 may be associated with vascular permeability , monocyte / macrophage infiltration , and rarefaction of peritubular capillaries , and the increase of the Ang1 / Ang2 ratio may be a compensatory mechanism counteracting the permeability inducing effect of VEGF after ang 2 infusion . . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
The purpose of this study is to investigate the association of ultra high molecular weight polyethylene ( UHMWPE ) particle induced inflammatory osteoclastogenesis and expression of RANK / RANKL and VEGF / VEGF receptors ( Flt 1 and Flk 1 ) using a mouse osteolysis model . ^^^ UHMWPE stimulation significantly increased VEGF gene expression , and exerted a lower enhancement effect on the gene expression of Flt 1 and Flk 1 . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Immunolocalizations of VEGF , its receptors flt 1 , KDR and TGF beta ' s in epithelial ovarian tumors . ^^^ In this study , we aimed to evaluate the immunolocalizations of vascular endothelial growth factor ( VEGF ) , its receptors flt 1 , KDR / flk 1 , and transforming growth factor beta ' s ( TGF beta ) in epithelial ovarian tumors , utilizing indirect immunohistochemistry to understand the role of the angiogenic events in ovarian neoplasia . ^^^ All formalin fixed , paraffin embedded tissue sections were stained with hematoxylin eosin or primary antibodies against VEGF , flt 1 , KDR / flk 1 , TGF beta 1 , TGF beta 2 and TGF beta 3 using the avidin biotin peroxidase method . ^^^ VEGF receptors , flt 1 and KDR / flk 1 immunoreactivities were detected not only in vascular endothelial cells , but also in tumor cells at malignant sites . ^^^ Conclusion : Our results suggest that overexpression of VEGF , its receptors flt 1 , KDR / flk 1 and TGF beta interaction may play an important role in the ovarian cancer biology , with potential effects on tumor growth and angiogenesis . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Immunocytochemical analysis of ESC derived endothelial cells ( EC ) demonstrates that both cell types are positive for the EC markers endothelial nitric oxide synthase ( eNOS ) , Flk 1 , Flt 1 , vascular endothelial cadherin ( VEcad ) , platelet endothelial cell adhesion molecule 1 ( PECAM 1 ) , and CD 34 . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Immunofluorescence or fluorescence activated cell sorter ( FACS ) analysis revealed that esECs expressed a full range of EC lineage specific markers including CD 31 , CD 106 , CD 144 , Flk 1 , Flt 1 , and von Willebrand factor ( vWF ) . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Recently , the gene encoding vascular permeability factor ( VPF ) or vascular endothelial growth factor ( VEGF ) and the genes encoding its receptors ( kinase insert domain containing receptor ( KDR ) and fms like tyrosine kinase 1 [ fit 1 ] ) have been cloned . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Fms like tyrosine kinase 1 ( Flk 1 ) showed two expression peaks on days 4 and 10 of culture . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
The KDR receptor tyrosine kinase shares structural similarities with a recently reported receptor for VEGF , flt , in a manner reminiscent of the similarities between the alpha and beta forms of the PDGF receptors . . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Expression of vascular endothelial growth factor and its receptors flt and KDR in ovarian carcinoma . ^^^ The known human receptors for VEGF , flt and KDR , are both cell surface tyrosine kinases and are expressed predominantly on endothelial cells . ^^^ METHODS : VEGF , flt , and KDR expression was localized by in situ hybridization and immunohistochemistry in frozen sections of primary tumors from five patients with ovarian carcinoma and from metastases of ovarian carcinoma from three different patients . ^^^ Reverse transcription followed by polymerase chain reaction ( RT PCR ) and an enzyme linked immunosorbent assay were used to analyze VEGF , flt , and KDR expression in six epithelial cell lines derived from ovarian carcinoma ascites from five additional patients . ^^^ RESULTS : Messenger RNAs ( mRNAs ) encoding VEGF , flt , and KDR were detected in primary ascitic cells and in three of four ovarian carcinoma cell lines examined by RT PCR . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Differential expression of the two VEGF receptors flt and KDR in placenta and vascular endothelial cells . ^^^ Screening for flt and KDR expression in a variety of species and tissue derived endothelial cells demonstrates that flt is predominantly expressed in human placenta and human vascular endothelial cells . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Vascular endothelial cell growth factor binds with high affinity to FLT and KDR , two homologous tyrosine kinase receptors expressed on vascular endothelial cells . ^^^ Placental growth factor , a vascular endothelial cell growth factor homologue , also binds with high affinity to the extracellular domains of FLT but not to the extracellular region of KDR . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
FLT 4 was found to contain an extracellular region similar to the corresponding sequences of FLT and Flk 1 , containing seven immunoglobulin domains . . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Quek 1 and Quek 2 show high homology to three interrelated murine and human genes , flk 1 , KDR and flt . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
The authors studied the hypoxic gene regulation of two known VEGF receptors ( KDR and Flt ) and its mechanism in cultured bovine retinal endothelial cells ( BREC ) . ^^^ KDR was detectable by Northern blot analysis in BREC , whereas Flt was not . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Localization of VEGF and expression of its receptors flt and KDR in human placenta throughout pregnancy . ^^^ Its action is mediated through the tyrosine kinase receptors flt and KDR . ^^^ These results indicate that VEGF may exert an important role within both the placental villi and the maternal decidua in relation to the growth , differentiation and migration of trophoblast and that this is mediated primarily through the spatial and temporal regulation of the flt receptor rather than the KDR receptor . . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
VEGF exerts its effect through two known high affinity tyrosine kinase receptors , named kinase insert domain containing receptor ( KDR ) and the fms like tyrosine kinase ( Flt ) . ^^^ In the present study , we demonstrate the expression of Flt , but not KDR , in bovine retinal pericytes ( BRPCs ) . ^^^ Although KDR is expressed predominantly in retinal endothelial cells , Northern blot analysis demonstrated substantial expression of the Flt gene in BRPCs without detection of KDR despite using polyadenylated RNA . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
VEGF receptors flt and KDR ( kinase domain receptor ) were also detected , suggesting autocrine regulation . ^^^ During the menstrual cycle , expression of flt was constant but that of KDR was increased in the luteal phase , at which time the cells migrated in response to VEGF . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
In this study , we have examined the expression of VEGF and its tyrosine kinase receptors ( flt and flk 1 ) in isolated rat islets of Langerhans in vitro . ^^^ Reverse transcriptase polymerase chain reaction revealed the presence of both flt and flk 1 in freshly isolated islets , and two VEGF isoforms , namely VEGF 120 and VEGF 164 . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Reverse transcriptase polymerase chain reaction analysis demonstrated the presence of both KDR and flt mRNA , two known VEGF receptors , in SMC cultures . ^^^ Western blot analysis of SMC lysates confirmed these data , revealing bands migrating at approximately 200 kDa and slightly below 200 kDa consistent with KDR and flt . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
A series of substituted 4 anilinoquinazolines and related compounds were synthesized as potential inhibitors of vascular endothelial growth factor ( VEGF ) receptor ( Flt and KDR ) tyrosine kinase activity . ^^^ Introduction of a hydroxyl group at the meta position of the aniline produced the most potent inhibitors of Flt and KDR tyrosine kinases activity with IC ( 50 ) values in the nanomolar range ( e . g . 10 , 12 , 13 , 16 , and 18 ) . ^^^ Our inhibitors proved to be very selective inhibitors of Flt and KDR tyrosine kinase activity when compared to that associated with the FGF receptor ( 50 to 3800 fold ) . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Because systemic hypertension increases vascular stretch , we evaluated the expression of VEGF , VEGF R 2 ( kinase insert domain containing receptor [ KDR ] ) , and VEGF R 1 ( fms like tyrosine kinase [ Flt ] ) in bovine retinal endothelial cells ( BRECs ) undergoing clinically relevant cyclic stretch and in spontaneously hypertensive rat ( SHR ) retina . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Specifically , 1 ) VEGF / Flt and Ang 1 / Tie 2 may promote trophoblast growth , 2 ) VEGF / KDR and Ang 1 / Tie 2 may support fetoplacental vascular development and stabilization , 3 ) sFlt may balance VEGF actions , and 4 ) Ang 2 / Tie 2 may remodel the maternal vasculature . . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
To inhibit vascular endothelial growth factor ( VEGF ) , a soluble combined truncated form of the fms like tyrosine kinase ( Flt ) and kinase insert domain containing receptor ( KDR ) receptor fused to IgG ( VEGF Trap R1R2 ) was administered for 10 d during the follicular phase of the cycle . ^^^ Effects on gene expression of VEGF and its receptors , Flt and KDR , were studied by in situ hybridization . ^^^ VEGF expression in granulosa and thecal cells increased after treatment , whereas Flt and KDR expressions in thecal endothelial cells were markedly decreased . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Vascular endothelial growth factor ( VEGF ) and its receptors , kinase insert domain containing region ( KDR ) and fms like tyrosine kinase ( Flt ) mRNA , were localized and quantified in in situ hybridization . ^^^ No major differences were found in expression of VEGF , Flt , or KDR in these CL . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
Training produced initial three to sixfold increases in VEGF , VEGF receptors ( KDR and Flt ) , the angiopoietin receptor ( Tie 2 ) , and endothelial nitric oxide synthase mRNA , which dissipated before the increase in capillarity , and a substantial ( 30 to 50 fold ) but transient upregulation of monocyte chemoattractant protein 1 mRNA . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
This review describes the preclinical and clinical status of low molecular weight drugs targeted against different tyrosine kinases ( e . g . , epidermal growth factor receptor [ EGFR ] , vascular endothelial growth factor receptor [ VEGFR ] , platelet derived growth factor receptor [ PDGFR ] , Kit , Fms like tyrosine kinase [ Flt ] 3 ) , briefly describes new targets , and provides a critical analysis of the current situation in the area of tyrosine kinase inhibitors . . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
METHODS : Normal and arterialized vein graft segments were evaluated by reverse transcription polymerase chain reaction ( RT PCR ) for expression of VEGF R 1 ( flt ) , VEGF R 2 ( KDR ) , and neuropilin 1 . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
To evaluate a possible contribution of the VEGF receptors , we performed immunohistochemical stainings of VEGF R 1 ( flt ) , R 2 ( KDR , flk ) , and Neuropilin 1 . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
In association with these cellular changes , mRNA for several angiogenic factors including vascular endothelial growth factor ( VEGF ) and receptors ( Flt and KDR ) , basic fibroblast growth factor ( FGF 2 ) and receptor , angiopoietin ( ANGPT ) 1 and receptor Tie 2 , endothelial nitric oxide synthase ( NOS 3 ) , and angiotensin 2 receptor 1 ( AT 1 ) were altered . ^^^ In addition , changes in mRNA expression for VEGF , Flt and KDR were positively correlated with changes in ANGPT 2 , Tie 2 , and NOS 3 , indicating a functional relationship . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
It has been postulated that PlGF is involved in intra and intermolecular cross talk between VEGF receptor 1 ( FLT ) and receptor 2 ( FLK 1 / KDR ) . ^^^
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
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Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
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Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
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Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
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Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
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Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
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Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
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Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
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Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
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Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
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Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
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Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
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Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
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Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
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Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
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Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
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Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
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Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
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Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
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Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
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Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
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Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
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Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
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Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
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Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
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Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
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Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
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Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
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Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
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Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
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Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
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Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
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Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
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Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
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Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
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Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
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Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
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Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
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Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
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Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
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Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
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Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
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Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
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Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
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Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
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Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
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Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
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Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
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Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
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Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
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Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
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Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
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Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
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Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
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Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
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Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
NA
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
NA
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
NA
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
NA
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
NA
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
NA
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
NA
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
NA
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
NA
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
NA
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
NA
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
NA
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
NA
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
NA
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
NA
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
NA
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
NA
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
NA
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
NA
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
NA
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
NA
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
NA
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
NA
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
NA
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
NA
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
NA
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
NA
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
NA
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
NA
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
NA
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
NA
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
NA
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
NA
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
NA
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
NA
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
NA
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
NA
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
NA
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
NA
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
NA
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
NA
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
NA
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
NA
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
NA
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
NA
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
NA
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
NA
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
NA
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
NA
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
NA
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
NA
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
NA
Interacting proteins: P17948 and P35968	Pubmed	SVM Score :0.0
NA