Pubmed abstracts for Protein-Protein Interaction search result :

Interacting proteins: P35858 and P17936	Pubmed	SVM Score :0.82335479
We present evidence that the binary complexes containing proteolytically nicked IGFBP 3 can be formed in two ways : by direct association of IGFBP 3 with ALS in the absence of IGF , and by proteolysis of IGFBP 3 within 150 kDa ternary complexes , resulting in increased dissociation of IGF 1 . 0.82335479^^^
Interacting proteins: P35858 and P17936	Pubmed	SVM Score :0.73834842
Contrary to traditional reports that ALS interacts only with IGF bound IGFBP 3 , this study shows that ALS is capable of forming a binary complex with IGFBP 3 in the absence of IGF . 0.73834842^^^ Little is known about how ALS binds to IGFBP 3 , which connects the IGFs to ALS . 0.66420677^^^
Interacting proteins: P35858 and P17936	Pubmed	SVM Score :0.57230283
Studies , however , indicate that ALS forms a doublet with IGFBP 3 , independent of IGFs . 0.57230283^^^
Interacting proteins: P35858 and P17936	Pubmed	SVM Score :0.0
Circulating insulin like growth factors ( IGFs ) represent an important pool of potential hypoglycemic activity , which is largely inhibited by their sequestration in a heterotrimeric complex comprising growth factor , IGF binding protein 3 ( IGFBP 3 ) , and acid labile subunit ( ALS ) . ^^^
Interacting proteins: P35858 and P17936	Pubmed	SVM Score :0.0
To further investigate the effects of IGF 1 administration upon the IGF IGFBP axis in GHRD , we have examined : 1 ) the molecular distribution of IGF 1 and IGF 2 among the IGFBPs ; 2 ) the composition and distribution of the IGFBPs , in particular IGFBP 3 ; and 3 ) the acid labile subunit ( ALS ) . ^^^ Administration of IGF 1 to GHRD patients was unable to increase concentrations of the molecular forms of IGFBP 3 , correct the aberrant distribution of IGFs among the IGFBPs , or increase serum concentrations of ALS . ^^^ In conclusion , we have found two forms of IGFBP 3 associated with IGF and ALS , which are capable of forming the ternary 150 kDa complex in healthy adult serum . ^^^
Interacting proteins: P35858 and P17936	Pubmed	SVM Score :0.0
Circulating insulin like growth factor ( IGF ) binding protein 3 ( IGFBP 3 ) , when occupied by IGF 1 or IGF 2 , combines with an acid labile glycoprotein subunit ( ALS ) to form a high molecular weight complex . ^^^ Purification involved ion exchange chromatography , and affinity chromatography on an IGF 1 IGFBP 3 column , yielding almost 1 mg ALS from 100 ml serum . ^^^ Rat ALS was almost as potent as human ALS in binding to human IGF 1 IGFBP 3 complex ( association constant , 2 . 3 nM 1 ) . ^^^
Interacting proteins: P35858 and P17936	Pubmed	SVM Score :0.0
Nearly all of the insulin like growth factor ( IGF ) in the circulation is bound in a heterotrimeric complex composed of IGF , IGF binding protein 3 , and the acid labile subunit ( ALS ) . ^^^ Expression of the cDNA clones in mammalian tissue culture cells results in the secretion into the culture medium of ALS activity that can form the expected complex with IGF 1 and IGF binding protein 3 . ^^^
Interacting proteins: P35858 and P17936	Pubmed	SVM Score :0.0
ALS activity was quantitated by the conversion of covalent BP 53 [ 125I ] IGF 1 tracer from about 60K to about 150K . ^^^ DEAE Sephadex chromatography of serum at pH 8 . 2 yielded separate peaks of 30 60K BP 53 immunoreactivity and 100 110K ALS which , when mixed , converted the BP 53 to 150K . ^^^ Whole serum also contained 100 110K ALS not complexed with BP 53 . ^^^ In an assay involving competition between test substances and BP 53 IGF 1 tracer in the reaction with partially purified ALS , serum samples , acidified to inactivate endogenous ALS , reacted with potencies proportional to their immunoreactive BP 53 content . ^^^
Interacting proteins: P35858 and P17936	Pubmed	SVM Score :0.0
Insulin like growth factors 1 and 2 ( IGF 1 and 2 ) are present in serum primarily within a ternary complex consisting of IGF , IGF binding protein 3 ( IGF 3 ) and acid labile subunit ( ALS ) . ^^^ Acidification of both whole serum and purified ALS leads to a complete loss of ALS ability to bind to cross linked IGFBP 3 : [ 125I ] IGF 2 tracer ; however , immunoblot studies show no change in the apparent M ( r ) of the major ALS band . ^^^
Interacting proteins: P35858 and P17936	Pubmed	SVM Score :0.0
Larger complexes ( > 95 kDa ) present in the nonacidified 150 kDa pool were not observed , consistent with the IGFBP 3 molecules in the flow through and eluate having been complexed to an acid labile subunit ( ALS ) . ^^^ We conclude that the 150 kDa fraction of adult rat serum includes 1 ) ternary complexes of intact IGFBP 3 ( which binds IGF 1 and IGF 2 with similar affinity ) , endogenous IGF 1 , and the ALS ; and 2 ) binary complexes of proteolytically nicked IGFBP 3 and ALS that bind IGF 2 preferentially . ^^^
Interacting proteins: P35858 and P17936	Pubmed	SVM Score :0.0
In the adult circulation , 70 90 % of the serum insulin like growth factors ( IGFs ) are carried by IGF binding protein 3 ( IGFBP 3 ) , which exists as part of a 150 kilodalton ( kDa ) ternary complex including IGF and an acid labile subunit ( ALS ) . ^^^ Thus , GH infusion was able to induce formation of the 150 kDa ternary complex by increasing circulating levels of IGF 1 , IGFBP 3 , and presumably ALS . ^^^ Administration of IGF 1 also increased serum IGF 1 and IGFBP 3 levels , although the increase in IGFBP 3 was only in the 60 kDa region of the chromatograph , suggesting that IGF 1 can induce neither ALS nor formation of the 150 kDa complex . ( ABSTRACT TRUNCATED AT 400 WORDS ) . ^^^
Interacting proteins: P35858 and P17936	Pubmed	SVM Score :0.0
Insulin like growth factor binding protein 3 ( IGFBP 3 ) , after first associating with IGF 1 or IGF 2 , is able to associate with the acid labile subunit ( ALS ) and form a 140 kDa complex . ^^^ Insulin like growth factor binding protein 3 ( IGFBP 3 ) , after first associating with IGF 1 or IGF 2 , is able to associate with the acid labile subunit ( ALS ) and form a 140 kDa complex . ^^^
Interacting proteins: P35858 and P17936	Pubmed	SVM Score :0.0
Insulin like growth factors ( IGFs ) are present in the circulation , largely as part of a high mol wt complex including IGF binding protein 3 ( IGFBP 3 ) and an acid labile subunit ( ALS ) . ^^^ IGFBP 3 transcripts are considerably more abundant and widely expressed than ALS at birth , but both are present in liver and kidney . ^^^ Hepatic IGFBP 3 gene expression increases slightly , whereas ALS increases dramatically in the first few weeks after birth . ^^^ IGFBP 3 mRNA is concentrated in portal venous and sinusoidal endothelium , but is not detected in hepatocytes , whereas ALS mRNA is diffusely expressed by hepatocytes , but is not detected in nonparenchymal cells . ^^^ Both transcripts are localized in the renal cortex ; however , IGFBP 3 mRNA is concentrated in interstitial cells , whereas ALS is expressed in proximal tubule epithelium . ^^^
Interacting proteins: P35858 and P17936	Pubmed	SVM Score :0.0
We have presently studied ternary complex formation between IGF , IGFBP 3 , and acid labile subunit ( ALS ) to further assess if IGF 1 bioavailability is increased in human fetal serum . ^^^
Interacting proteins: P35858 and P17936	Pubmed	SVM Score :0.0
The acid labile subunit ( ALS ) is a glycoprotein that forms a ternary complex in serum with insulin like growth factors and insulin like growth factor binding protein 3 . ^^^
Interacting proteins: P35858 and P17936	Pubmed	SVM Score :0.0
The molecular size distribution of IGFBP 3 in rhGH treated rats was similar to that of normal rats ( IGFBP 3 in the 150K mol wt range ) , due to rhGH increasing serum ALS , but was altered by rhIGF 1 ( IGFBP 3 in the 200 300K and 44K mol wt range ) . ^^^ In a GH deficient animal , restoring the IGF / IGFBP 3 / ALS axis towards normal is associated with greater growth promotion . . ^^^
Interacting proteins: P35858 and P17936	Pubmed	SVM Score :0.0
IGFBP 3 , the major circulating IGFBP , is unique in combining with a glycoprotein , the acid labile subunit ( ALS ) , to form a ternary complex with IGF 1 or IGF 2 . ^^^
Interacting proteins: P35858 and P17936	Pubmed	SVM Score :0.0
On the basis of these data , ALS seems to have a more complex function than that of simply increasing the mol wt of the IGF IGFBP 3 complex . . ^^^
Interacting proteins: P35858 and P17936	Pubmed	SVM Score :0.0
In these patients the formation of serum complexes between IGFs , IGF binding protein 3 ( IGFBP 3 ) , and the acid labile subunit ( ALS ) is impaired . ^^^ Both prednisolone and GH alleviated the hypoglycemia , concomitantly with increases in IGF 1 , IGFBP 3 , and ALS levels . ^^^ However , only prednisolone improved the ability of IGFBP 3 to bind ALS in vitro . ^^^ Compared with normal IGF 2 , pro IGF 2 inhibited ALS binding to IGFBP 3 in vitro . ^^^ We conclude that the failure of serum IGFBP 3 and tumor IGF 2 to complex with ALS is a primary cause of hypoglycemia in nonislet cell tumor hypoglycemia . . ^^^
Interacting proteins: P35858 and P17936	Pubmed	SVM Score :0.0
Although recent studies demonstrate that a number of cell types in culture secrete IGFs and IGFBP 3 , very little is known with regard to the origin of circulating ALS . ^^^ To test the hypothesis that human bone cells ( HBCs ) , which produce abundant amounts of IGF 2 and IGFBP 3 , also produce ALS , we measured the IGF 1 , IGF 2 , IGFBP 3 , and ALS levels using specific radioimmunoassays ( RIAs ) in the conditioned medium ( CM ) of untransformed normal HBCs and SaOS 2 osteosarcoma cells treated with various effectors ( IGF 2 , osteogenic protein 1 [ OP 1 , bone morphogenetic protein 7 ] and human GH ) for 48 h . ^^^ In the IGF 2 ( 3 , 30 ng / ml ) treated HBC CM , the IGFBP 3 level was increased in a dose dependent manner but neither IGF 1 nor ALS could be detected . ^^^ In the SaOS 2 cell culture , OP 1 ( 1 , 100 ng / ml ) increased both IGF 2 and IGFBP 3 secretion but neither ALS nor IGF 1 secretion . ^^^ Treatment of HBCs with GH ( 1 , 10 , 100 ng / ml ) had no significant effect on the secretion of either IGF 1 , IGF 2 , IGFBP 3 , or ALS . ( ABSTRACT TRUNCATED AT 250 WORDS ) . ^^^
Interacting proteins: P35858 and P17936	Pubmed	SVM Score :0.0
The high capacity binding protein for IGFs in blood is IGF binding protein 3 ( IGFBP 3 ) , which is largely complexed with an acid labile subunit ( ALS ) . ^^^ However , we and others have shown that IGFBP 3 not complexed with ALS can rapidly leave the circulation , and may carry IGFs to peripheral tissues . ^^^
Interacting proteins: P35858 and P17936	Pubmed	SVM Score :0.0
IGF binding protein 3 ( IGFBP 3 ) , the major serum carrier of IGF 1 and IGF 2 in adults , circulates predominantly in a ternary complex with IGF and the acid labile subunit ( ALS ) . ^^^ IGF binding protein 3 ( IGFBP 3 ) , the major serum carrier of IGF 1 and IGF 2 in adults , circulates predominantly in a ternary complex with IGF and the acid labile subunit ( ALS ) . ^^^ ALS binding peaked at pH 5 . 5 6 . 0 , and was markedly reduced at pH 7 . 4 , where only approximately 0 . 4 mol ALS was bound per mol IGFBP 3 , with greatly reduced affinity . ^^^ Under ' physiological ' conditions , the affinities of ALS binding to IGF 1 IGFBP 3 and IGF 2 IGFBP 3 complexes ( 2 . 5 10 10 ( 8 ) l / mol and 5 . 8 10 10 ( 7 ) l / mol , respectively ) are 300 fold and 2000 fold lower than the constants for the formation of the corresponding binary complexes . ^^^ Thus , ALS binding to IGFBP 3 complexes is much weaker than previously recognised , emphasising the importance of ALS dissociation as a controlling factor in the regulation of IGF bioavailability . . ^^^
Interacting proteins: P35858 and P17936	Pubmed	SVM Score :0.0
Serum levels of insulin like growth factor 1 ( IGF 1 ) , IGF binding protein 3 ( IGFBP 3 ) , and the acid labile subunit ( ALS ) were measured in 46 GHD men participating in a 1 yr , double blind , and placebo controlled dose response study . ^^^ Significant increases in IGF 1 , IGFBP 3 , and ALS levels were observed with a dose as low as 0 . 33 IU / M ( 2 ) day . ^^^ The minimal GH dose required for normalization of the serum IGF 1 concentration was 0 . 66 IU / M ( 2 ) day , and it was 1 . 0 IU / M ( 2 ) day for ALS and IGFBP 3 . ^^^ In patients receiving 2 . 0 IU / M ( 2 ) day , the mean serum IGF 1 concentration rose to an abnormally high level , whereas at this dose , the mean IGFBP 3 and ALS levels were not different from normal . ^^^ The lower sensitivity of IGFBP 3 and ALS to GH doses in the high range was also apparent during long term treatment . ^^^
Interacting proteins: P35858 and P17936	Pubmed	SVM Score :0.0
Within the liver , the biosynthesis of individual proteins has been attributed to different cell populations , e . g . , that of ALS to hepatocytes and that of IGFBP 3 to nonparenchymal cells . ^^^
Interacting proteins: P35858 and P17936	Pubmed	SVM Score :0.0
GH therapy resulted in a normalization of the levels of blood sugars , IGFBP 3 , ALS , IGFBP 2 , and IGF 1 , as well as the IGF / IGFBP 3 ratio . ^^^ We speculate that as a result of the decreased IGFBP 3 and ALS levels , the IGF population was shifted from the stable 150K complex to lower molecular weight complexes with IGF binding proteins , increasing IGF availability to tissues due to rapid turnover of these low molecular weight complexes . ^^^
Interacting proteins: P35858 and P17936	Pubmed	SVM Score :0.0
In mammals IGF 1 is part of a 150 kDa binding protein complex , which also contains a glycosylated acid labile protein ( ALS ) and a glycosylated acid stable IGF binding subunit IGFBP 3 . ^^^
Interacting proteins: P35858 and P17936	Pubmed	SVM Score :0.0
In contrast , the ternary complex and its components , IGF 1 , IGFBP 3 and ALS increased with gestation . ^^^ We show that while ALS is an important limiting factor for ternary complex formation in the fetal circulation , there is a fraction of IGFBP 3 which is unable to form this complex . ^^^
Interacting proteins: P35858 and P17936	Pubmed	SVM Score :0.0
IGFBP 3 binds IGF and a third glycoprotein , the acid labile subunit ( ALS ) , to form a stable 150 kDa ternary complex that serves as an intravascular store for IGFs and prolongs IGF half life . ^^^
Interacting proteins: P35858 and P17936	Pubmed	SVM Score :0.0
In addition , radioimmuno assays for insulin like growth factor 1 ( IGF 1 ) , IGF binding protein 3 ( IGFBP 3 ) , acid labile subunit ( ALS ) and IGFBP 1 were performed . ^^^ IGFBP 3 levels rose to double the pretreatment value , but dropped to normal levels following transplantation , while ALS rose with rhGH treatment and remained increased after transplantation . ^^^
Interacting proteins: P35858 and P17936	Pubmed	SVM Score :0.0
The circulating insulin like growth factors ( IGF 1 and 2 ) occur largely as components of a 140 kDa protein complex with IGF binding protein 3 and the acid labile subunit ( ALS ) . ^^^
Interacting proteins: P35858 and P17936	Pubmed	SVM Score :0.0
In the circulation , most of the IGFs are bound to a high molecular weight binding protein complex of 150 kDa that consists of IGF 1 ( or IGF 2 ) , IGFBP 3 and the acid labile subunit ( ALS ) . ^^^ Although expressed in different liver cell populations IGFBP 3 and ALS were regulated synergistically . ^^^ Insulin stimulated both the expression of ALS and IGFBP 3 in co cultures in a dose dependent manner , while expression of IGFBP 1 was decreased . ^^^
Interacting proteins: P35858 and P17936	Pubmed	SVM Score :0.0
Circulating IGF 1 exists primarily as part of a ternary 150 kDa complex comprising equimolar amounts of IGF 1 , IGFBP 3 and acid labile subunit ( ALS ) . ^^^ It is also known that in contrast to IGF 1 and IGFBP 3 there exists a substantial quantity of unbound ALS in the circulation . ^^^ Systematic administration of rhIGF I / IGFBP 3 results in binding of this binary complex to endogenous free ALS and thus leads to increased circulating levels of 150 kD ternary complex ( IGF I / IGFBP 3 / ALS ) . ^^^ In addition , the pharmacokinetic profile suggests that saturation of excess ALS occurs at considerably lower doses of rhIGF I / IGFBP 3 in the monkey than in the rat . ^^^ In addition , IGFBP 3 increases the therapeutic index of rhIGF 1 even at doses that significantly exceed the saturation of ALS . . ^^^
Interacting proteins: P35858 and P17936	Pubmed	SVM Score :0.0
After 12 months of study , the 30 children receiving rhGH showed : ( 1 ) greater increase in height ( 9 . 1 + / 2 . 8 vs . 5 . 5 + / 1 . 9 cm , P < 0 . 0001 ) ; ( 2 ) increases in serum levels of IGF 1 , IGF 2 , free IGF 1 , IGFBP 3 and acid labile subunit ( ALS ) ; ( 3 ) a greater decrease in serum IGFBP 1 levels ; and ( 4 ) no significant difference in serum IGFBP 2 levels , when compared to the 14 control patients . ^^^ The change in height SDS after 12 months of rhGH ( +0 . 8 ) in the 30 treated children correlated significantly and positively with serum ALS , IGFBP 3 , total IGF , IGF 1 , IGF 2 and free IGF 1 levels measured during treatment . ^^^
Interacting proteins: P35858 and P17936	Pubmed	SVM Score :0.0
The liver appears to be the main source of the three components of the 150 kDa complex and , in particular , hepatocytes synthesize the insulin like growth factor ( IGF ) peptide and the acid labile subunit ( ALS ) , whereas Kupffer and sinusoidal endothelial cells produce IGF binding protein 3 ( IGFBG 3 ) . ^^^
Interacting proteins: P35858 and P17936	Pubmed	SVM Score :0.0
In NICTH , IGFs and IGF binding protein 3 ( IGFBP 3 ) are mainly found in binary complexes of 50 60 kD , instead of the normal ternary complex of about 140 kD with the acid labile subunit ( ALS ) . ^^^ Factors contributing to the defect are : ( 1 ) low ALS levels , secondary to suppressed growth hormone ( GH ) ; ( 2 ) defective IGFBP 3 binding to ALS ; ( 3 ) reduced ability of pro IGF 2 forms to complex normally , and ( 4 ) very high levels of other IGFBPs , including IGFBP 2 and IGFBP 6 , which might limit the formation of complexes with IGFBP 3 . ^^^ Both treatments increase serum ALS , IGFBP 3 , and IGF 1 levels , and decrease IGFBP 2 , whereas IGFBP 6 is unaffected . ^^^
Interacting proteins: P35858 and P17936	Pubmed	SVM Score :0.0
Serum levels of insulin like growth factor 1 ( IGF 1 ) , IGF binding protein 3 ( IGFBP 3 ) , the acid labile subunit ( ALS ) , insulin , and IGFBP 1 were evaluated as indicators of body composition during GH replacement therapy in 20 GH deficient patients ( 9 women and 11 men ) , aged 22 57 yr , with IGF 1 levels below 2 SD . ^^^ Serum levels of IGF 1 , ALS , and IGFBP 3 above the normal range were reached in seven , five , and three subjects , respectively , after 12 months of GH therapy . ^^^ IGF 1 and ALS levels , but not IGFBP 3 levels , correlated with the total daily GH dose ( r = 0 . 676 ; P = 0 . 001 and r = 0 . 631 ; P = 0 . 003 ) . ^^^ At 12 months , the LBM values were significantly correlated to the IGF 1 levels ( r = 0 . 718 ; P < 0 . 001 ) , but not to ALS or IGFBP 3 levels . ^^^
Interacting proteins: P35858 and P17936	Pubmed	SVM Score :0.0
Gel filtration and immunoprecipitation studies performed on blood collected 2 min after injection confirmed that the injected hIGFBP 3 ( 46 82 % as much as rat IGFBP 3 ) was associated with ALS in 150 kDa complexes . ^^^ After i . v . injection into adult rats , human insulin like growth factor binding protein 3 ( hIGFBP 3 ) forms 150 kDa complexes with excess endogenous rat acid labile subunit ( ALS ) within 2 min ( Lewitt et al . , 1993 , Endocrinology 133 : 1797 ) . ^^^
Interacting proteins: P35858 and P17936	Pubmed	SVM Score :0.0
Most of the IGF 1 in plasma circulates in a 150 kDa complex that contains IGF binding protein 3 ( IGFBP 3 ) and an acid labile subunit ( ALS ) . ^^^
Interacting proteins: P35858 and P17936	Pubmed	SVM Score :0.0
Among the well defined insulin like growth factor ( IGF ) binding proteins ( IGFBPs ) , IGFBP 3 is characterized by its interaction with an acid labile glycoprotein ( ALS ) in the presence of IGFs . ^^^ We conclude that residues 228 232 of IGFBP 3 are essential for cell association and are required for normal ALS binding affinity . . ^^^
Interacting proteins: P35858 and P17936	Pubmed	SVM Score :0.0
In addition , there is increased proteolysis of IGFBP 3 ( AIDS failure to thrive , uncontrolled diabetes mellitus ) and disruption of the ternary complex with decreased levels of ALS ( AIDS wasting and burns ) . ^^^
Interacting proteins: P35858 and P17936	Pubmed	SVM Score :0.0
Serum ALS fractionated by gel filtration high performance liquid chromatography eluted in a single peak at approximately 150 kDa with IGF 1 and IGFBP 3 , but appeared at about 400 500 kDa after sample acidification and fractionation under acidic condition . ^^^ ALS in adult sera more tightly correlated with IGFBP 3 than IGF 1 or IGF 2 . ^^^
Interacting proteins: P35858 and P17936	Pubmed	SVM Score :0.0
Additionally , serum contains a structurally unrelated protein , acid labile sub unit ( ALS ) , which forms a ternary complex with IGF and IGFBP 3 . ^^^
Interacting proteins: P35858 and P17936	Pubmed	SVM Score :0.0
ALS buffers the metabolic effects of the insulin like growth factors by sequestering them in a ternary complex with insulin like growth factor binding protein 3 . ^^^
Interacting proteins: P35858 and P17936	Pubmed	SVM Score :0.0
IGFBP 3 was measured by RIA and Western ligand blotting , ALS by RIA . ^^^ Based values of IGFBP 3 and ALS were low . ^^^ We conclude that IGF 1 treatment induces and initial suppression and then an increase in the IGFBP 3 and ALS concentrations , confirming data from animal experiments that IGFBP 3 synthesis is not solely under GH control . ^^^
Interacting proteins: P35858 and P17936	Pubmed	SVM Score :0.0
Reduced pulsatile fraction of TSH , GH , and PRL secretion and low serum concentrations of T 4 , T 3 , insulin growth factor 1 ( IGF 1 ) , IGF binding protein 3 ( IGFBP 3 ) , and the acid labile subunit ( ALS ) were documented in the untreated state . ^^^ Pulsatile GH secretion was amplified > 6 and > 10 fold , respectively , by GHRP 2 and GHRH + GHRP 2 infusions , generating mean increases of serum IGF 1 ( 66 % and 106 % ) , IGFBP 3 ( 50 % and 56 % ) , and ALS ( 65 % and 97 % ) within 45 h . ^^^
Interacting proteins: P35858 and P17936	Pubmed	SVM Score :0.0
ALS buffers the growth and metabolic effects of the insulin like growth factors by sequestering them in a ternary complex with IGFBP 3 . ^^^
Interacting proteins: P35858 and P17936	Pubmed	SVM Score :0.0
Analysis of the distribution of IGF 1 , IGF 2 and IGFBP 3 in fluid from healthy and atretic follicles revealed that the majority of these growth factors ( > 80 % of total IGF 2 ) were in the 150KDa complex , indicating that the ALS present was functional , in that it formed the ternary complex with a molecule of IGFBP 3 and IGF . ^^^
Interacting proteins: P35858 and P17936	Pubmed	SVM Score :0.0
We describe a novel competitive assay for rat insulin like growth factor ( IGF ) binding protein 3 ( rIGFBP 3 ) based on the ability of IGFBP 3 to form a ternary complex with the acid labile subunit ( ALS ) in the presence of IGF . ^^^
Interacting proteins: P35858 and P17936	Pubmed	SVM Score :0.0
MEASUREMENTS : Circulating levels of total IGF 1 , free and bound IGF 1 , Insulin , IGFBP 1 , IGFBP 3 , ALS , catecholamines and potassium were measured . ^^^ There was no significant change in IGFBP 3 or ALS during the IGF 1 or insulin infusions . ^^^ IGF 1 and insulin under conditions of adequate substrate supply have acute effects on IGFBP 1 and potassium physiology , but have little effect on IGFBP 3 , ALS or catecholamines . . ^^^
Interacting proteins: P35858 and P17936	Pubmed	SVM Score :0.0
In postnatal animals , most of circulating IGFs occur in 150 kDa complexes formed by association of an acid labile subunit ( ALS ) with complexes of IGF and IGF binding protein 3 . ^^^
Interacting proteins: P35858 and P17936	Pubmed	SVM Score :0.0
We have therefore studied the effects of a single subcutaneous dose of rhIGF 1 ( 40 micrograms / kg at 1800 h ) , when compared with an untreated control night , in 17 subjects with IDDM , on serum concentrations of IGF 1 , IGF 2 , IGFBP 3 , acid labile subunit ( ALS ) , and IGFBP 3 proteolysis . ^^^ On the baseline night , IGFBP 3 levels correlated with the sum of IGF 1 and IGF 2 ( r = 0 . 73 , P = 0 . 02 ) and with levels of the ALS ( r = 0 . 7 , P = 0 . 002 ) . ^^^ However after rhIGF 1 , the sum of IGF 1 and IGF 2 no longer correlated with IGFBP 3 , whereas the relationship with ALS was maintained . ^^^ In conclusion , despite a slight but significant fall in ALS , IGFBP 3 levels rise after rhIGF 1 administration in IDDM . ^^^ This can not be explained by alterations in IGFBP 3 proteolysis , and may relate to the relative stability of ALS / IGFBP 3 when complexed principally with IGF 1 rather than IGF II . . ^^^
Interacting proteins: P35858 and P17936	Pubmed	SVM Score :0.0
GH treatment significantly increased serum levels of total IGF 1 from 98 ( 68 ) to 323 ( 126 ) microg / l , free IGF 1 from 0 . 48 ( 0 . 47 ) to 2 . 24 ( 1 . 66 ) microg / l , IGFBP 3 from 1 , 874 ( 1 , 178 ) to 3 , 520 ( 778 ) microg / l and ALS levels from 9 , 182 ( 5 , 524 ) to 16 , 872 ( 6 , 278 ) microg / l ( all p < 0 . 0001 ) . ^^^
Interacting proteins: P35858 and P17936	Pubmed	SVM Score :0.0
The first aim of this project was to examine the molecular forms of IGFBP 3 and the acid labile subunit ( ALS ) , and to assess the changes in these molecular forms during GH administration to GHD children . ^^^ The response to GH therapy , as measured by the height velocity standard deviation score ( SDS ) adjusted for bone age , correlated with the percent change in total IGFBP 3 ( r = 0 . 772 , p = 0 . 002 by WIB ) , intact IGFBP 3 ( r = 0 . 845 , p = 0 . 0005 by WLB ; r = 0 . 541 , p = 0 . 05 by WIB ) , and proteolyzed IGFBP 3 ( r = 0 . 703 , p = 0 . 007 ) , as well as with the percent change in ALS ( r = 0 . 813 , p = 0 . 014 ) . ^^^ The second aim of this project was to assess the changes in distribution of the immunoreactive forms of IGFBP 3 and IGF 1 among the ternary ( ALS / IGFBP 3 / IGF ) complex , the binary ( IGFBP 3 / IGF ) complex , and uncomplexed IGF during the first year of GH therapy , and to explore further the correlation with growth response to GH . ^^^ In conclusion , GH treatment of GHD children results in significant increases of intact , proteolyzed , and total IGFBP 3 , as well as an increase in ALS , which all correlate with the growth response to GH therapy . ^^^
Interacting proteins: P35858 and P17936	Pubmed	SVM Score :0.0
Serum concentrations of leptin , insulin , cortisol , T 4 , T 3 , insulin like growth factor ( IGF ) 1 , IGF binding protein 3 and the acid labile subunit ( ALS ) were measured at 0900 h , 2100 h , and 0600 h on each of the 2 study days . ^^^
Interacting proteins: P35858 and P17936	Pubmed	SVM Score :0.0
We have recently described a competitive binding assay for rat insulin like growth factor binding protein 3 ( IGFBP 3 ) based on the ability of IGFBP 3 to form a ternary complex with the acid labile subunit ( ALS ) in the presence of IGF 1 . ^^^ Nonfasting serum levels of IGFBP 3 were compared with those of total ( extractable ) IGF 1 ( tIGF 1 ) and ALS as well as IGFBP 3 determined by ligand blotting . ^^^ IGFBP 3 correlated positively with tIGF 1 ( r = 0 . 90 ; P < 0 . 0001 ) , ALS ( r = 0 . 92 ; P < 0 . 0001 ) , and IGFBP 3 , as determined by ligand blotting ( r = 0 . 88 ; P < 0 . 0001 ) . ^^^ We conclude that in rats 1 ) IGFBP 3 and fIGF 1 are strongly age dependent ; 2 ) IGFBP 3 correlates positively with ALS and tIGF 1 ; and 3 ) fIGF 1 and IGFBP 1 are inversely correlated . ^^^
Interacting proteins: P35858 and P17936	Pubmed	SVM Score :0.0
Because IGF binding protein 3 ( IGFBP 3 ) binds to ALS through its basic carboxyl terminal domain , we tested whether a homologous region present in IGFBP 5 is involved in IGFBP 5 binding to ALS . ^^^ In addition , in the absence of IGFs , IGFBP 5 , a synthetic peptide representing the basic carboxyl terminal sequence IGFBP 5 ( 201 218 ) , and the corresponding IGFBP 3 basic sequence IGFBP 3 ( 215 232 ) , competed weakly for ALS binding to covalent IGF IGFBP 5 complex , as did a random sequence synthetic peptide with the same composition as IGFBP 5 ( 201 218 ) . ^^^
Interacting proteins: P35858 and P17936	Pubmed	SVM Score :0.0
In adults the IGFs circulate primarily with IGF binding protein 3 ( IGFBP 3 ) and an acid labile glycoprotein ( ALS ) in a 140 kDa complex which limits IGF bioavailability . ^^^ Circulating levels of IGF 1 , IGFBP 3 and ALS , and their degree of association in the ternary complex in the fetus increased with gestational age . ^^^ In neonatal serum from deliveries < 35 weeks ' gestation IGFBP 3 was predominantly in 30 50 kDa form ( s ) and ALS was a limiting factor for ternary complex formation . ^^^ In serum from deliveries > 35 weeks both ALS and IGFs were limiting but approximately 25 % of IGFBP 3 was unable to form the ternary complex even in the presence of excess ALS and IGF 1 . ^^^
Interacting proteins: P35858 and P17936	Pubmed	SVM Score :0.0
The main IGFBP in serum , IGFBP 3 , is known to form a 150 kDa complex with IGFs and the acid labile subunit ( ALS ) . ^^^ Serum ALS migrated separately ( at 88 kDa ) from the novel IGFBP 3 APs ( as evident by Western immunoblot ) , and bound IGFBP 3 weakly ( by reverse ligand blots ) . ^^^ We also demonstrated that large amounts of one of the IGFBP 3 APs and small amounts of ALS were coimmunoprecipitated with IGFBP 3 from human serum . ^^^ Similar to ALS , these IGFBP 3 APs are acid labile and lose their IGFBP 3 binding capacity after exposure to low pH . ^^^ We conclude that there are several serum proteins in addition to ALS and IGFs that bind IGFBP 3 with high affinity . ^^^
Interacting proteins: P35858 and P17936	Pubmed	SVM Score :0.0
Over 75 % of the circulating insulin like growth factors ( IGF 1 and 2 ) are bound in 140 kDa ternary complexes with IGF binding protein 3 ( IGFBP 3 ) and the 84 86 kDa acid labile subunit ( ALS ) , a glycoprotein containing 20 kDa of carbohydrate . ^^^ Complete deglycosylation abolished the ability of ALS to associate with IGFBP 3 . ^^^ Ionic interactions are known to be involved in the association between ALS and IGFBP 3 . ^^^ Desialylation reduced the affinity of ALS for IGFBP 3 . ^^^
Interacting proteins: P35858 and P17936	Pubmed	SVM Score :0.0
Ternary complex formation by the glycovariants in the presence of ALS and excess IGF 1 was not significantly different from that of fully glycosylated recombinant human ( rh ) IGFBP 3 [ Ka ( fully glycosylated ) =12 . 5+ / 4 . 1 l / nmol ; mean Ka ( all mutants ) =22 . 1+ / 3 . 0 l / nmol ] . ^^^ Therefore , while the carbohydrate units appear to be non essential to ALS or IGF binding , they may modulate other biological activities of IGFBP 3 . . ^^^
Interacting proteins: P35858 and P17936	Pubmed	SVM Score :0.0
Chronic liver disease was characterised by low serum IGF 1 , IGF 2 , IGFBP 3 and ALS levels with raised IGFBP 1 and 2 levels . ^^^
Interacting proteins: P35858 and P17936	Pubmed	SVM Score :0.0
Serum concentrations of IGFBP 3 , 4 and 5 and acid labile subunit ( ALS ) were therefore examined in 20 children with growth hormone ( GH ) insensitivity before and after 6 months of therapy with recombinant human IGF 1 ( 80 or 120 micrograms / kg twice daily ) . ^^^ Serum levels of IGFBP 3 , 4 and 5 and ALS all increased significantly ( p < 0 . 0001 ) in GH deficient children in response to GH therapy , whereas no significant increases occurred in the children with GH insensitivity . ^^^ These findings indicate that GH is responsible for the regulation of serum levels of IGFBP 3 , 4 and 5 and ALS , and that IGF 1 does not directly regulate the concentrations of these circulating IGFBPs . . ^^^
Interacting proteins: P35858 and P17936	Pubmed	SVM Score :0.0
Measurements of ALS and IGFBP 3 added no useful extra information to IGF 1 in managing the dose titration . ^^^
Interacting proteins: P35858 and P17936	Pubmed	SVM Score :0.0
Circulating insulin like growth factor 1 ( IGF 1 ) is predominantly bound in the trimeric complex comprised of IGF binding protein 3 ( IGFBP 3 ) and acid labile subunit ( ALS ) . ^^^ Circulating concentrations of IGF 1 , IGFBP 3 and ALS are believed to reflect the GH secretory status , but the clinical use of ALS determination is not known . ^^^ We found : 1 ) no significant arteriovenous gradient over the liver ofALS , IGF 1 , and IGFBP 3 ; 2 ) the diurnal variation of ALS was 12 % ( mean coefficient of variation percent ) ; 3 ) ALS levels increased throughout childhood with maximal levels in puberty , with a subsequent decrease with age in adults ; and 4 ) ALS levels were below 2 SD in 57 of 79 GHD patients ( sensitivity 72 % ) and above 2 SD in 22 of 29 patients with normal GH response ( specificity 76 % ) , which was similar , compared with the diagnostic utility of IGF 1 and IGFBP 3 . ^^^ Finally , our findings indicate that hepatic ALS production is not measurable by this approach or , alternatively , that the liver is not the primary source of circulating ALS , IGF 1 , or IGFBP 3 in humans . ^^^
Interacting proteins: P35858 and P17936	Pubmed	SVM Score :0.0
In human circulation , IGFs are mostly present as an approximately 150 kDa ternary protein complex consisting of IGFs , IGFBP 3 , and acid labile subunit ( ALS ) . ^^^ The present study suggested that ALS was regulated by nutritional status , the same as IGF 1 , IGFBP 2 and IGFBP 3 ; but the serum IGFBP 2 was the best predictor of BMI as nutritional status marker among the parameters in patients with anorexia nervosa . . ^^^
Interacting proteins: P35858 and P17936	Pubmed	SVM Score :0.0
Insulin like growth factors ( IGF 1 , free IGF 1 and IGF 2 ) and insulin like growth factor binding proteins ( IGFBP 2 , IGFBP 3 , IGFBP 6 , and ALS ) in blood circulation . ^^^ Overall , IGF 1 , IGFBP 3 and ALS were slightly higher in females than in males . ^^^ IGFs , IGFBP 3 and ALS were 5 10 % higher in serum than in plasma . ^^^
Interacting proteins: P35858 and P17936	Pubmed	SVM Score :0.0
However , little is known about how ALS binds to IGFBP 3 or 5 , which link the IGFs to ALS . ^^^ The internal face is lined with a substantial region of electronegative surface potential that could interact with the positively charged region on IGFBP 3 known to be involved in ALS binding . ^^^ The model also predicts that three potential N linked oligosaccharide sites within the LRR domain are clustered together , which may be important in light of recent studies showing ALS glycan involvement in complex formation with IGFBP 3 . . ^^^
Interacting proteins: P35858 and P17936	Pubmed	SVM Score :0.0
Most of the insulin like growth factors ( IGFs ) in adult rat or human plasma circulate in 150 kDa heterotrimeric complexes with IGF binding protein 3 ( IGFBP 3 ) and an acid labile subunit ( ALS ) . ^^^ We report that ALS mRNA is expressed in term fetal rat liver at 30 % of the levels in adult liver , that radioiodinated rat ALS is not proteolyzed by incubation with fetal rat serum , and that sufficient functional ALS is present in fetal rat serum to form 150 kDa complexes with recombinant human IGFBP 3 . ^^^
Interacting proteins: P35858 and P17936	Pubmed	SVM Score :0.0
Fasting ALS , IGF 2 , IGFBP 3 , IGFBP 2 , IGFBP 1 , and insulin levels before rhIGF 1 treatment were 46 . 3 mg / L , 1044 microg / L , 5 . 8 mg / L , 73 ng / mL , 4 . 7 ng / mL , and 27 . 3 mU / L , respectively . ^^^ Similar to healthy individuals , this patient had normal IGFBP 3 and ALS levels , which are the major regulators of the pharmacokinetics of rhIGF 1 . ^^^
Interacting proteins: P35858 and P17936	Pubmed	SVM Score :0.0
Insulin like growth factors ( IGFs ) in the circulation are predominantly sequestered into ternary complexes comprising IGF , IGF binding protein 3 ( IGFBP 3 ) , and the acid labile subunit ( ALS ) . ^^^ IGFBP 3 ( Ad ) had similar affinities for IGF 1 and ALS and an approximately 25 % decreased affinity for IGF 2 compared to IGFBP 3 ( Pl ) . ^^^
Interacting proteins: P35858 and P17936	Pubmed	SVM Score :0.0
Measurements of insulin like growth factor 1 ( IGF 1 ) , IGF binding protein 3 ( IGFBP 3 ) , and the acid labile subunit ( ALS ) are important in assessing the GH IGF axis . ^^^ As nearly all IGF 1 , IGFBP 3 , and ALS circulate in a GH dependent ternary protein complex , direct determination of the complex may be of significant analytical and clinical importance . ^^^ In pairwise mixed antibody evaluation , mAbs in G 2 and G 3 showed simultaneous binding to serum IGFBP 3 complexes in combination with an anti IGF 1 or an anti ALS antibody . ^^^ On this basis , two novel enzyme linked immunosorbent assays ( ELISAs ) involving IGFBP 3 / IGF 1 ( ELISA 1 ) and IGFBP 3 / ALS ( ELISA 2 ) recognition partners were developed , both demonstrating acceptable analytical performance characteristics . ^^^ IGFBP 3 complexes measured by ELISA 1 and 2 in samples from normal individuals and subjects with GH deficiency , acromegaly , and GH receptor deficiency more tightly correlated with IGF 1 , IGFBP 3 , and ALS than IGF 2 . ^^^
Interacting proteins: P35858 and P17936	Pubmed	SVM Score :0.0
Fasting reduced serum levels of GH , free and total IGF 1 , IGFBP 3 , and ALS , whereas IGFBP 1 was increased ( P < 0 . 0001 ) . ^^^ Free IGF 1 changed in parallel with total IGF 1 , ALS , and IGFBP 3 , being normalized at 48 h of refeeding . ^^^ However , the normal inverse relationship between free IGF 1 and IGFBP 1 was absent , and free IGF 1 changed in parallel with total IGF 1 and thus ALS and IGFBP 3 . ^^^
Interacting proteins: P35858 and P17936	Pubmed	SVM Score :0.0
Total IGF 1 level in serum is a sensitive index during growth hormone ( GH ) replacement therapy of adults , since GH stimulates the hepatic expressions of both insulin like growth factor ( IGF 1 ) and acid labile subunit ( ALS ) and the major part of IGF 1 in the circulation is found in a ternary complex together with ALS and IGFBP 3 . ^^^ It is not clarified , whether the IGF 1 levels within normal range for age is due to endogenous basal GH production being sufficient or other factors stimulating IGF 1 , IGFBP 3 or ALS expressions . . ^^^
Interacting proteins: P35858 and P17936	Pubmed	SVM Score :0.0
Body composition , IGF 1 , IGFBP 3 and ALS levels were measured before and after 3 months of rhGH therapy . ^^^ Body composition changed as expected most impressively in twin 1 ; twins 1 and 2 had low IGF 1 , IGFBP 3 and ALS prior to treatment with GH , but normal concentrations that were similar to those of their healthy twin were attained after 3 months of GH treatment . ^^^ Twin 3 had normal IGF 1 , IGFBP 3 and ALS concentrations before GH treatment , which were similar to those of her twin . ^^^ Insulin and GH have both been shown to stimulate hepatic production of IGF 1 and these results suggest that insulin may also be involved in the regulation of IGFBP 3 and ALS in vivo . ^^^ Insulin has already been implicated in the regulation of IGFBP 3 and ALS in vitro and there is some evidence to suggest that this is also the case in vivo . ^^^
Interacting proteins: P35858 and P17936	Pubmed	SVM Score :0.0
We therefore measured IGF 1 , IGF 2 , IGF binding protein 1 ( IGFBP 1 ) , IGFBP 2 , IGFBP 3 , and acid labile subunit ( ALS ) in 27 GHD subjects ( aged 5 82 yr ) from an extended kindred in Northeast Brazil with an identical GHRH receptor mutation and in 55 indigenous controls ( aged 5 80 yr ) . ^^^ IGF 1 in the ternary complex was determined principally by concentrations of ALS in GHD and IGFBP 3 in controls , implying that ALS has greater GH dependency . ^^^ Severe GHD not only reduces the amounts of IGFs , IGFBP 3 , and ALS , but also modifies the distribution of the IGFs bound to each IGFBP . ^^^
Interacting proteins: P35858 and P17936	Pubmed	SVM Score :0.0
Tumour derived IGF 2 has a higher than normal molecular weight ( big ' IGF 2 ' ) and an impaired ability to form the normal ternary 150 kD complex with IGF binding protein 3 ( IGFBP 3 ) and the acid labile subunit ( ALS ) . ^^^ Patient serum contained low levels of IGF 1 , IGFBP 3 and ALS , while the concentrations of IGFBP 2 and IGFBP 6 were markedly elevated . ^^^
Interacting proteins: P35858 and P17936	Pubmed	SVM Score :0.0
IGF 1 and free IGF 1 concentrations increased , whereas IGF binding protein 3 ( IGFBP 3 ) and acid labile subunit ( ALS ) were unchanged during treatment . ^^^ There was no correlation between absorption or terminal half life of IGF 1 and IGFBP 3 or ALS , but negative correlations with IGF 1 clearance ( CL / F ) and volume of distribution ( V / F ) . ^^^ Positive correlations between both IGFBP 3 and ALS and IGF 1 maximal concentration ( C ( max ) ) and time of C ( max ) ( T ( max ) ) were observed . ^^^ Additionally , IGFBP 3 and ALS concentrations modulate the peak concentrations of IGF 1 achieved and correlate reciprocally with its V / F and CL / F , underscoring the critical importance of binding proteins in modulating the bioavailability of IGF 1 in vivo in humans . . ^^^
Interacting proteins: P35858 and P17936	Pubmed	SVM Score :0.0
Recombinant human GH treatment ( 0 . 15 IU / kg 10 day ) for 1 week increased serum total IGF 1 , IGFBP 3 , and ALS , exaggerating the responses to exercise . ^^^ Total IGF 1 , IGFBP 3 , and ALS returned to baseline over 3 4 days . ^^^ In summary , 1 ) acute exercise transiently increased all components of the IGF 1 ternary complex , possibly due to mobilization of preformed intact complexes ; 2 ) GH pretreatment augmented the exercise induced changes in ternary complexes ; 3 ) postexercise IGFBP 1 increments may protect against delayed onset hypoglycemia ; 4 ) serum total IGF 1 , IGFBP 3 , and ALS may be suitable markers of GH abuse ; and 5 ) differences in disappearance times altered the sensitivity of each marker for detecting GH abuse . . ^^^
Interacting proteins: P35858 and P17936	Pubmed	SVM Score :0.0
Similar to healthy individuals , this patient had normal IGFBP 3 but elevated ALS levels . ^^^
Interacting proteins: P35858 and P17936	Pubmed	SVM Score :0.0
During extrauterine life , insulin like growth factors ( IGFs ) circulate in a ternary serum complex with one IGF binding protein 3 ( IGFBP 3 ) or IGFBP 5 protein and with a single acid labile subunit ( ALS ) . ^^^
Interacting proteins: P35858 and P17936	Pubmed	SVM Score :0.0
Specifically the reduced amount of GH released in pulses is found to be related to low circulating levels of IGF 1 , IGFBP 3 and acid labile subunit ( ALS ) , which suggests that a relative hyposomatotropism may participate in the pathogenesis of the wasting syndrome distinctively in the chronic phase of critical illness . ^^^ The relative hyposomatotropism seems at least in part of hypothalamic origin since the whole somatotropic axis has been found to be very responsive to continuous infusion of GH releasing peptide ( GHRP ) , administered alone or in combination with GH releasing hormone ( GHRH ) , as evidenced by reactivated pulsatile GH secretion followed by substantial increases in circulating levels of IGF 1 , IGFBP 3 and ALS . ^^^
Interacting proteins: P35858 and P17936	Pubmed	SVM Score :0.0
Like insulin like growth factor binding protein 3 ( IGFBP 3 ) , IGFBP 5 forms a ternary complex with insulin like growth factor ( IGF ) 1 or IGF 2 , and the acid labile subunit ( ALS ) . ^^^
Interacting proteins: P35858 and P17936	Pubmed	SVM Score :0.0
The Ati Negritos showed lower growth hormone binding protein ( GHBP ) , insulin like growth factor 1 ( IGF 1 ) , insulin like growth factor binding protein 3 ( IGFBP 3 ) , acid labile subunit ( ALS ) , zinc , albumin , ferritin , iron , iron saturation and much higher insulin like growth factor binding protein 2 ( IGFBP 2 ) and plasma transferrin concentrations . ^^^
Interacting proteins: P35858 and P17936	Pubmed	SVM Score :0.0
The aim of our study was to determine the effects of 36 h fasting on 8 h diurnal GH , insulin and glucose levels as well as on basal IGF 1 , IGFBP 3 , acid labile subunit ( ALS ) , IGFBP 1 , GHBP and free fatty acid ( FFA ) levels . ^^^ STUDY DESIGN : In all subjects we studied the effects of 36 h fasting on 8 h daytime GH , insulin and glucose levels ( assay every 30 min from 0800 h to 1600 h ) as well as on basal IGF 1 , IGFBP 3 , ALS , IGFBP 1 , GHBP and FFA levels . ^^^ RESULTS : Before fasting , basal mean IGF 1 , IGFBP 3 and ALS levels in GHD were lower ( P < 0 . 0001 ) than in NS . ^^^ After fasting , both in GHD and NS basal IGF 1 , IGFBP 3 , ALS and GHBP levels did not change significantly . ^^^
Interacting proteins: P35858 and P17936	Pubmed	SVM Score :0.0
After 1 week off GH treatment , significantly lower levels of IGF 1 , IGF 2 , IGFBP 3 , and ALS were recorded ( P < 0 . 001 ) . ^^^
Interacting proteins: P35858 and P17936	Pubmed	SVM Score :0.0
Serum IGF 1 , IGFBP 3 , and acid labile subunit ( ALS ) levels were low in patients compared with controls , with male patients revealing lower IGF 1 ( P = 0 . 01 ) and ALS ( P = 0 . 005 ) concentrations than female patients . ^^^
Interacting proteins: P35858 and P17936	Pubmed	SVM Score :0.0
Therefore , ALS is required for postnatal accumulation of IGF 1 and IGFBP 3 but , consistent with findings supporting a predominant role for locally produced IGF 1 , is not critical for growth . ^^^
Interacting proteins: P35858 and P17936	Pubmed	SVM Score :0.0
BACKGROUND / AIMS : In the circulation , insulin like growth factor 1 ( IGF 1 ) is bound in a trimeric complex of 150 kDa with IGF binding protein 3 ( IGFBP 3 ) and the acid labile subunit ( ALS ) . ^^^ Whereas circulating IGF 1 and IGFBP 3 are reported to be low in patients with chronic liver failure , the level of ALS has not been described in relation to hepatic dysfunction . ^^^ During a haemodynamic investigation , blood samples were collected from the hepatic vein and femoral artery , and the plasma concentrations of ALS , IGF 1 and IGFBP 3 were determined . ^^^ ALS correlated significantly with indicators of liver dysfunction , including the Child Turcotte score ( r= 0 . 69 , p < 0 . 0001 ) , IGF 1 ( r=0 . 82 , p < 0 . 0001 ) and IGFBP 3 ( r=0 . 74 , p < 0 . 0001 ) . ^^^
Interacting proteins: P35858 and P17936	Pubmed	SVM Score :0.0
In adult animals , most circulating insulin like growth factor 1 ( IGF 1 ) and IGF 2 is sequestered in a 150 kDa complex composed of 1 molecule each of IGF , IGF binding protein 3 or 5 , and the acid labile subunit ( ALS ) . ^^^
Interacting proteins: P35858 and P17936	Pubmed	SVM Score :0.0
It remains to be determined whether IGF 1 directly affects hepatic synthesis and secretion of IGFBP 3 and what role IGF 1 has in the direct regulation of ALS in the monkey . . ^^^
Interacting proteins: P35858 and P17936	Pubmed	SVM Score :0.0
As previously described , ternary complex formation by acid labile subunit ( ALS ) with IGFBP 3 and pro IGF 2 was greatly decreased compared with complex formation with mature IGF 2 . ^^^
Interacting proteins: P35858 and P17936	Pubmed	SVM Score :0.0
IGF 1 and IGFBP 3 concentrations were determined by radioimmunoassays and acid labile subunit ( ALS ) was measured by ELISA . ^^^ A shift in distribution of IGFBP 3 and IGF 1 in RA and OA synovial fluids ( RASynF , OASynF ) and an associated increase in ALS suggested the presence of 150 kDa ternary complexes . ^^^
Interacting proteins: P35858 and P17936	Pubmed	SVM Score :0.0
IGF 1 circulates as a ternary complex with IGF binding protein 3 ( IGFBP 3 ) and the acid labile subunit ( ALS ) . ^^^ It is not known whether IGFBP 3 and ALS in blood are regulated by estrogen and , if so , whether this is also route dependent . ^^^ In the present study we investigate the effects on IGFBP 3 and ALS of oral and transdermal estrogens ( study 1 ) , of different oral estrogen formulations ( ethinyl estradiol , conjugated estrogen , and estradiol valerate ; study 2 ) , of different estrogen dosages ( study 3 ) in normal postmenopausal women , and of oral estrogen in hypogonadal GH deficient women ( study 4 ) . ^^^ Administration of oral , but not transdermal , estrogen in normal postmenopausal women significantly decreased serum levels of IGFBP 3 and ALS ( P < or = 0 . 005 ) . ^^^ In hypogonadal GH deficient women , oral estrogen treatment also significantly reduced IGFBP 3 and ALS ( P = 0 . 02 ) . ^^^
Interacting proteins: P35858 and P17936	Pubmed	SVM Score :0.0
The 140 kDa ternary complex of insulin like growth factor binding protein 3 ( IGFBP 3 ) , IGFs and an acid labile subunit ( ALS ) has previously been shown to be decreased in diabetes mellitus in humans and rats . ^^^
Interacting proteins: P35858 and P17936	Pubmed	SVM Score :0.0
Almost all of the serum IGFs are found in a ternary complex composed of IGF , IGFBP 3 and acid labile subunit ( ALS ) . ^^^ Serum ALS levels were significantly correlated with serum IGF 1 levels and IGFBP 3 levels . ^^^
Interacting proteins: P35858 and P17936	Pubmed	SVM Score :0.0
After birth , the acid labile subunit ( ALS ) associates in the circulation with insulin like growth factor ( IGF ) 1 or 2 and with IGF binding protein 3 ( IGFBP 3 ) to form a 150 kilodalton complex . ^^^ This growth depression is associated with large decreases in the plasma concentrations of both IGF 1 and IGFBP 3 , indicating the critical role of ALS in the regulation of circulating levels of these proteins . ^^^
Interacting proteins: P35858 and P17936	Pubmed	SVM Score :0.0
The production by the liver of the three subunits of the growth hormone ( GH ) dependent 150 kDa complex ( IGF 1 , IGF binding protein 3 and acid labile subunit or ALS ) is primarily under the control of GH . ^^^
Interacting proteins: P35858 and P17936	Pubmed	SVM Score :0.0
Levels of IGF 1 , IGF 2 , IGF binding protein 3 ( IGFBP 3 ) and acid labile subunit ( ALS ) were low on admission , and in the 8 patients whose serum IGF 1 levels failed to increase over 30 days , levels of the other proteins also remained low , while IGFBP 3 proteolytic activity increased . ^^^
Interacting proteins: P35858 and P17936	Pubmed	SVM Score :0.0
Among the six insulin like growth factor binding proteins ( IGFBP ) , only IGFBP 3 and IGFBP 5 form ternary complexes with IGFs and the acid labile sunbunit ( ALS ) . ^^^
Interacting proteins: P35858 and P17936	Pubmed	SVM Score :0.0
Insulin like growth factors ( IGFs ) circulate in plasma as part of a 150 kD complex that also contains IGF binding protein 3 ( IGFBP 3 ) , a protein that binds IGF 1 and IGF 2 with high affinity , and an acid labile subunit ( ALS ) that does not directly bind IGFs . ^^^ Significant correlations ( p < 0 . 001 ) were seen between total and free ALS concentrations and IGF 1 ( r = 0 . 50 and 0 . 60 , respectively ) , free IGF 1 ( r = 0 . 37 and 0 . 36 ) , IGF 2 ( r = 0 . 37 and 0 . 27 ) , IGFBP 1 ( r = 0 . 48 and 0 . 49 ) , IGFBP 2 ( r = 0 . 44 and 0 . 51 ) and IGFBP 3 ( r = 0 . 67 and 0 . 59 ) at all Tanner stages . ^^^
Interacting proteins: P35858 and P17936	Pubmed	SVM Score :0.0
It has recently been shown that , in follicular fluid , as in the circulation , insulin like growth factors ( IGFs ) 1 and 2 exist in a ternary complex with IGF binding protein 3 ( IGFBP 3 ) and the acid labile subunit ( ALS ) . ^^^ The current study was designed to determine whether ovarian follicular and luteal cells could synthesize IGFBP 3 and ALS . ^^^ We studied IGFBP 3 and ALS messenger RNA ( mRNA ) by in situ hybridization . ^^^ In thecal cells , expression of mRNA for IGFBP 3 , ALS and cyclin D 1 ( a marker of cell proliferation ) was restricted to healthy ( aromatase expressing ) follicles . ^^^ This study demonstrates that follicular fluid IGFBP 3 and ALS , like the IGFs , originate ( at least in part ) from the ovary . ^^^
Interacting proteins: P35858 and P17936	Pubmed	SVM Score :0.0
In the circulation insulin like growth factor 1 ( IGF 1 ) , IGF binding protein 3 ( IGFBP 3 ) , and the acid labile subunit ( ALS ) form a 150 kDa ternary complex that is of importance for the regulation of IGF 1 bioactivity . ^^^
Interacting proteins: P35858 and P17936	Pubmed	SVM Score :0.0
The change in ALS with growth hormone ( GH ) therapy correlated closer with the change in IGF 1 ( r=0 . 798 , p=0 . 0057 ; Spearman rank correlation ) than with the change in insulin like growth factor binding protein ( IGFBP 3 ; r=0 . 549 , p=0 . 057 ) . ^^^
Interacting proteins: P35858 and P17936	Pubmed	SVM Score :0.0
Body composition ( dual energy 10 ray absorptiometry ) , resting VO 2 ( indirect calorimetry ) , resting HR ( telemetry ) and serum IGF 1 , IGF 2 , IGFBP 3 and acid labile subunit ( ALS ) were measured at baseline , after 12 weeks of GH administration and , additionally in the GH group , 1 , 2 , 3 , 4 , 5 and 9 days after GH discontinuation . ^^^ Resting VO 2 and resting HR increased by 31 + / 3 . 6 % and 7 . 3 + / 1 . 9 per minute , respectively , in the GH group , where significant increases in serum IGF 1 , IGFBP 3 and ALS also were noted . ^^^ CONCLUSIONS : The increases in serum IGF 1 , IGFBP 3 , ALS and resting heart rate induced by 12 weeks of GH administration in elderly men returned to baseline levels within 2 3 days after GH discontinuation . ^^^
Interacting proteins: P35858 and P17936	Pubmed	SVM Score :0.0
STUDY DESIGN : We studied the effects of 36 h fasting on 8 h diurnal mean GH , insulin and glucose concentrations ( mGHc , mINSc and mGLUc ; assay every 30 min from 8 . 00 am to 4 . 00 pm ) as well as on IGF 1 , IGFBP 3 , ALS , IGFBP 1 , GHBP and free fatty acid ( FFA ) levels . ^^^ IGFBP 3 and ALS levels did not change . ^^^
Interacting proteins: P35858 and P17936	Pubmed	SVM Score :0.0
IGF 1 circulates predominantly as a ternary complex with IGF binding protein 3 ( IGFBP 3 ) and the acid labile subunit ( ALS ) . ^^^ In the treatment of active acromegaly with the GH receptor antagonist pegvisomant , ALS showed a closer correlation with the change in ring size , measured as a clinical indicator of disease activity , than did IGF 1 or IGFBP 3 . ^^^
Interacting proteins: P35858 and P17936	Pubmed	SVM Score :0.0
At d 130 of fetal life , fetuses suffering from chronic undernutrition caused by placental insufficiency had lower expression of the ALS and IGF 1 genes than well nourished fetuses , but they did not have any changes in the expression of the IGF binding protein ( IGFBP ) 2 or IGFBP 3 genes . ^^^ The lower plane of nutrition decreased the expression of the ALS , IGF 1 , and GH receptor genes and increased the expression of the IGFBP 2 gene ; expression of the IGFBP 3 gene was not affected by nutrition at this stage of life . ^^^
Interacting proteins: P35858 and P17936	Pubmed	SVM Score :0.0
Serum IGF 1 , IGF 2 , IGFBP 3 , and total and free ALS levels were significantly decreased at diagnosis . ^^^ Normalization of IGF 2 and IGFBP 3 occurred 6 months after diagnosis , and normalization of IGF 1 and total and free ALS occurred 1 yr after terminating therapy . ^^^
Interacting proteins: P35858 and P17936	Pubmed	SVM Score :0.0
We conclude that 1 ) males are significantly more responsive than females to exogenous GH ; 2 ) the increase in IGF 1 is more robust compared with those in IGFBP 3 and ALS ; 3 ) IGFBP 2 changes very little during GH treatment ; and 4 ) among IGF related substances , IGF 1 is the most specific marker of supraphysiological GH exposure . . ^^^
Interacting proteins: P35858 and P17936	Pubmed	SVM Score :0.0
BACKGROUND / AIMS : In healthy adults , serum insulin like growth factor 1 ( IGF 1 ) , IGF binding protein 3 ( IGFBP 3 ) and acid labile subunit ( ALS ) form a 150 kDa ternary complex under the control of growth hormone ( GH ) . ^^^ METHODS : Serum IGF 1 , IGFBP 3 , ALS , the 150 kDa ternary complex and IGFBP 3 proteolysis were all measured in six compensated and six decompensated cirrhotic patients and compared to six healthy controls . ^^^ RESULTS : Patients with compensated cirrhosis had decreased levels of IGF 1 ( 55 % ) , IGFBP 3 ( 64 % ) and ALS ( 53 % ) , and in the decompensated patients these levels were decreased even further : IGF 1 ( 32 % ) , IGFBP 3 ( 37 % ) and ALS ( 27 % ) compared to healthy controls . ^^^
Interacting proteins: P35858 and P17936	Pubmed	SVM Score :0.0
Candidate biochemical markers include insulin like growth factor 1 ( IGF 1 ) , IGF binding protein 3 ( IGFBP 3 ) and the acid labile subunit ( ALS ) , and markers of bone remodelling . ^^^ Serum IGF 1 has the greatest utility during GH dose titration as it is more sensitive to changes in GH status than IGFBP 3 and ALS , and is also more sensitive to excessive GH replacement . ^^^
Interacting proteins: P35858 and P17936	Pubmed	SVM Score :0.0
Indeed , researchers have shown that IGF 1 levels are of greater diagnostic value than other possible markers , such as IGF binding protein 3 ( IGFBP 3 ) and the acid labile subunit ( ALS ) . ^^^
Interacting proteins: P35858 and P17936	Pubmed	SVM Score :0.0
Most serum IGF 1 associates with IGF binding protein 3 ( IGFBP 3 ) and another protein , the acid labile subunit ( ALS ) . ^^^ Like IGF 1 , serum concentrations of IGFBP 3 and ALS are tightly regulated by GH . ^^^ GH secretion ( both spontaneous and stimulated ) , IGF 1 , IGFBP 3 , and ALS have been assessed as potential biochemical markers for diagnosis of GH related disorders . ^^^
Interacting proteins: P35858 and P17936	Pubmed	SVM Score :0.0
Compared to wild type , male PPARalpha / mice had 40 % lower total fasting IGF 1 concentrations , decreased ALS and less IGFBP 3 ternary complex formation , but within 4 h of refeeding there was an increase in IGF 1 and IGFBP 3 ternary complex to values similar to controls . ^^^
Interacting proteins: P35858 and P17936	Pubmed	SVM Score :0.0
Efficacy of radiotherapy in normalizing serum IGF 1 , acid labile subunit ( ALS ) and IGFBP 3 levels in acromegaly . ^^^ Moreover , data regarding other markers of disease activity , such as IGFBP 3 and acid labile subunit ( ALS ) , i . e . the other two components of the circulating 150 kDa complex , are lacking . ^^^ Serum GH and IGF 1 levels off medical therapy were measured in all patients ; ALS and IGFBP 3 were measured in 11 patients with normalization of IGF 1 concentrations . ^^^ ALS and IGFBP 3 concentrations paralleled IGF 1 levels in all patients studied . ^^^
Interacting proteins: P35858 and P17936	Pubmed	SVM Score :0.0
Serum IGFBP 5 levels were significantly associated with ALS levels ( r = 0 . 478 ; P = 0 . 008 ) , but the association was less than that between IGFBP 3 and ALS ( r = 0 . 743 ; P < 0 . 001 ) , reflecting the lower percentage of IGFBP 5 complexed with ALS . ^^^
Interacting proteins: P35858 and P17936	Pubmed	SVM Score :0.0
RESULTS : Patients revealed suppressed pulsatile GH , TSH and LH secretion in the face of low serum concentrations of IGF 1 , IGFBP 3 and the acid labile subunit ( ALS ) ( P < 0 . 0001 each ) , thyroid hormones ( P < 0 . 0001 ) and total and estimated free testosterone ( P < 0 . 0001 ) levels , whereas free oestradiol ( E 2 ) estimates were normal . ^^^ GHRP 2 infusion reactivated GH secretion and normalized serum IGF 1 , IGFBP 3 and ALS . ^^^
Interacting proteins: P35858 and P17936	Pubmed	SVM Score :0.0
RESULTS : Birth weight ( BW ) , birth length ( BL ) , ponderal index , head circumference ( HC ) , and cord serum levels of albumin , prealbumin , retinol binding protein ( RBP ) , total and free IGF 1 , IGF 2 , IGFBP 3 , acid labile subunit ( ALS ) , and leptin were significantly lower , whereas levels of IGFBP 1 , IGFBP 2 , and IL 6 were significantly higher in preterm than in term neonates ( P < 0 . 05 ) . ^^^
Interacting proteins: P35858 and P17936	Pubmed	SVM Score :0.0
Instead , western immunoblotting and immunoprecipitation experiments showed a 150 kDa IGFBP 3 acid labile subunit ( ALS ) complex in the anterior pituitary and not in the posterior pituitary and other brain regions . ^^^ Our results indicate that the human anterior pituitary contains a not previously described tightly cell membrane bound 150 kDa IGFBP 3 ALS complex that is absent in brain and posterior pituitary . . ^^^
Interacting proteins: P35858 and P17936	Pubmed	SVM Score :0.0
In serum , insulin like growth factors ( IGFs ) are primarily present as a approximately 150 kDa ternary protein complex , which consists of IGFs , IGF binding protein 3 ( IGFBP 3 ) , and acid labile subunit ( ALS ) . ^^^ Like IGF 1 and IGFBP 3 , serum levels of ALS depend on growth hormone ( GH ) . ^^^ We also measured the levels of serum IGF 1 and IGFBP 3 in these patients to compare the utility of ALS with IGF 1 and IGFBP 3 as a marker of GH secretion . ^^^ Serum ALS was measured by radioimmunoassay ( RIA ) kit , and serum IGF 1 and IGFBP 3 were measured by immunoradiometric assay ( IRMA ) kits in 56 patients with adult GHD ( adult onset ( AO ) / child onset ( CO ) , 13 / 43 ) and 43 patients with acromegaly . ^^^ These data suggested that measurement of ALS offers no advantage over measurements of serum IGF 1 and IGFBP 3 . ^^^
Interacting proteins: P35858 and P17936	Pubmed	SVM Score :0.0
In serum , the majority of the IGFs exist in a 150 kDa complex including the IGF molecule , IGF binding protein 3 ( IGFBP 3 ) , and the acid labile subunit ( ALS ) . ^^^ Thus , the double gene disruption LID+ALSKO mouse model demonstrates that a threshold concentration of circulating IGF 1 is necessary for normal bone growth and suggests that IGF 1 , IGFBP 3 , and ALS play a prominent role in the pathophysiology of osteoporosis . . ^^^
Interacting proteins: P35858 and P17936	Pubmed	SVM Score :0.0
We studied serum ALS , free IGF 1 , total IGF 1 , IGFBP 3 levels and IGFBP 3 protease activity in 13 girls , aged 1 . 6 7 . 8 yr ( mean , 5 . 9 + / 2 . 2 ) , diagnosed as having CPP before and after 6 and 12 months of GnRH analog ( GnRHa ) therapy . ^^^
Interacting proteins: P35858 and P17936	Pubmed	SVM Score :0.0
Acid labile subunit ( ALS ) showed diagnostic merit similar to insulin like growth factor binding protein 3 ( IGFBP 3 ) with PV ( pos ) of 0 . 69 and 0 . 71 and PV ( neg ) of 0 . 91 and 0 . 92 respectively . ^^^ Total IGF 1 , IGFBP 3 and ALS possess a higher PV ( neg ) for the clinical disease activity . ^^^
Interacting proteins: P35858 and P17936	Pubmed	SVM Score :0.0
We characterized the GH IGF 1 axis by analyzing the individual components of the axis ( GH , IGF 1 , IGF binding protein 3 [ IGFBP 3 ] , acid labile subunit [ ALS ] ) and the corresponding ratios ( GH / IGF 1 , GH / IGFBP 3 , and GH / ALS ) and verified the links with circulating proinflammatory cytokines ( TNF alpha , IL 1beta , and IL 6 ) , in 34 patients with CLD and 12 healthy controls . ^^^
Interacting proteins: P35858 and P17936	Pubmed	SVM Score :0.0
IGF 1 circulates in free , ternary [ IGF 1 + IGFBP 3 + acid labile subunit ( ALS ) ] , and binary ( IGF 1 + IGFBP ) molecular complexes , and the relative proportions regulate IGF 1 extravascular shifting and bioavailability . ^^^ Components of the IGF 1 system ( total and free IGF 1 ; IGFBP 1 , 3 , and ALS ; nonternary IGF 1 and IGFBP 3 ) , biomarkers of metabolic and nutritional status ( transferrin , ferritin , prealbumin , glucose , free fatty acids , glycerol , beta hydroxybutyrate ) , and body composition were measured in 12 men ( 22 + / 3 yr , 87 + / 8 kg , 183 + / 7 cm , 20 + / 5 % body fat ) on days 1 , 3 , and 4 during a control and experimental ( Exp ) period . ^^^ IGF 1 and IGFBP 3 were measured by immunoassay before and after immunoaffinity depletion of ALS based complexes ( i . e . , ternary complex removal ) . ^^^
Interacting proteins: P35858 and P17936	Pubmed	SVM Score :0.0
In these mice the decrease in IGFBP 3 levels is associated with impaired formation of the 150 kD ternary complex , even in the presence of normally functional ALS . ^^^
Interacting proteins: P35858 and P17936	Pubmed	SVM Score :0.0
RESULTS : Basal serum levels of IGF 1 ( P < 0 . 0001 ) and IGFBP 3 ( P < 0 . 01 ) declined with age , but serum acid labile subunit ( ALS ) levels did not ( P = 0 . 2 ) . ^^^ However , the increment in IGF 1 , IGFBP 3 and ALS in response to GH did not decline with age . ^^^
Interacting proteins: P35858 and P17936	Pubmed	SVM Score :0.0
METHODS : Viral load , CD 4 count , interleukin 6 ( IL 6 ) , growth hormone , insulin like growth factor 1 ( IGF 1 ) , IGF binding protein 3 ( IGFBP 3 ) , acid labile subunit ( ALS ) , IGFBP 3 proteolysis , osteocalcin in blood and N terminal telopeptide of type 1 collagen in urine were determined . ^^^ Low IGF 1 was found in only six children , who had low CD 4 count and high IL 6 levels , with normal levels of IGFBP 3 and ALS , absent IGFBP 3 proteolysis and decreased bone mineral density , irrespective of viral load or growth . ^^^
Interacting proteins: P35858 and P17936	Pubmed	SVM Score :0.0
The concentrations of the following markers were determined in relevant serum samples : IGF 1 , IGFBP 3 , ALS , PIIINP , PINP , osteocalcin , and leptin . ^^^
Interacting proteins: P35858 and P17936	Pubmed	SVM Score :0.0
BACKGROUND / AIM : In healthy adults , serum insulin like growth factor 1 ( IGF ) , IGF binding protein 3 ( IGFBP 3 ) , and acid labile subunit ( ALS ) form a 150 kD ternary complex under the control of growth hormone ( GH ) . ^^^ Despite GH hypersecretion , serum IGF 1 , IGFBP 3 , and ALS levels have all been reported to be low in patients with anorexia nervosa ( AN ) , while the degree of ternary complex formation in AN is unknown . ^^^ RESULTS : Patients with AN had low levels of ALS and IGFBP 3 contained in the 150 kD ternary complex and in the non 150 kD fraction . ^^^
Interacting proteins: P35858 and P17936	Pubmed	SVM Score :0.0
Dominant negative STAT5b completely inhibited GH stimulated transcription of genes encoding the growth promoting proteins IGF 1 , IGF binding protein 3 ( IGFBP 3 ) , and acid labile subunit ( ALS ) , which comprise the major circulating IGF 1 complex , and blocked expression of the GH inhibitors SOCS 1 , SOCS 2 , and CIS , but had little effect on induction of SOCS 3 . ^^^ Constitutive active STAT5b stimulated robust transcription of IGF 1 , ALS , and IGFBP 3 in the absence of hormone but did little to modify GH mediated activation of SOCS family genes . ^^^
Interacting proteins: P35858 and P17936	Pubmed	SVM Score :0.0
In training subjects GH treatment increased GH , IGF 1 , IGFBP 3 and ALS , and decreased IGBP 2 . ^^^ After cessation of treatment IGF 1 , IGFBP 3 and ALS approached basal values between 49 and 96 h . ^^^ In conclusion , increases in IGF 1 , IGFBP 3 , ALS , PIIIP and ICTP are all indicative of recent GH abuse or of acromegaly . . ^^^
Interacting proteins: P35858 and P17936	Pubmed	SVM Score :0.0
MEASUREMENTS : We analysed the strength and independence of associations of angiographically assessed presence of CHD with BMI , systolic and diastolic blood pressure , total , high density lipoprotein ( HDL ) and LDL cholesterol , triglycerides , lipoprotein ( a ) , apolipoproteins A 1 and B , total and free IGF 1 , IGF 2 , IGFBP 1 , IGFBP 3 , IGFBP 5 , acid labile subunit ( ALS ) , insulin , C peptide , testosterone , DHEAS and sex hormone binding globulin . ^^^ RESULTS : Using multivariate statistical analysis , the presence of CHD had significant positive associations with total IGF 1 , IGFBP 5 , ALS and IGFBP 3 . ^^^
Interacting proteins: P35858 and P17936	Pubmed	SVM Score :0.0
Big IGF 2 suppresses growth hormone ( GH ) biosynthesis and impairs the storage of IGFs by suppressing the formation of the GH dependent ternary complexes containing IGF , IGF binding protein 3 ( IGFBP 3 ) , and acid labile subunit ( ALS ) . ^^^ Prednisone monotherapy partially suppressed big IGF 2 secretion , and rhGH monotherapy acted on IGFBP 3 and ALS concentrations . ^^^
Interacting proteins: P35858 and P17936	Pubmed	SVM Score :0.0
Acid labile subunit ( ALS ) is a component of the 150 kDa insulin like growth factor binding protein 3 ( IGFBP 3 ) complex , which , by sequestering the majority of IGFs 1 and 2 and thereby prolonging the half life of them in plasma , serves as a circulating reservoir of IGFs in mammalian species . ^^^
Interacting proteins: P35858 and P17936	Pubmed	SVM Score :0.0
IGF 1 , IGFBP 3 and ALS are GH dependent peptides and their production is disturbed in states of GH insensitivity . ^^^ This chapter explores the relative degrees of IGF 1 , IGFBP 3 and ALS deficiency across the spectrum of GH insensitivity . ^^^ In classical GH insensitivity syndrome ( GHIS ) , known as Laron syndrome , due to GH receptor ( GHR ) deficiency , serum IGF 1 , IGFBP 3 and ALS are severely reduced with inability to produce these peptides during an IGF 1 generation test . ^^^ In a new series of GHIS cases ( n = 36 ) there was a significant difference in IGFBP 3 and ALS ( p < 0 . 05 ) between classical ( n = 25 ) and non classical cases ( n = 11 ) . ^^^ IGF 1 , IGFBP 3 and ALS were significantly higher ( p < 0 . 05 ) in pubertal compared with pre pubertal subjects in the same series . ^^^
Interacting proteins: P35858 and P17936	Pubmed	SVM Score :0.0
We demonstrated that the level of IGF 1 and ALS mRNAs is dramatically decreased in both young and aged dwarf livers , transcripts encoding IGF IR and IGFBP 1 are elevated in young dwarfs , but normalize to control levels in aged dwarf livers while transcripts encoding IGFBP 3 are elevated only in aged controls . ^^^
Interacting proteins: P35858 and P17936	Pubmed	SVM Score :0.0
These complexes are referred to as binary complexes consisting of IGF 1 or IGF 2 and one IGFBP , or as ternary complexes each consisting of either of IGF 1 or IGF 2 , IGFBP 3 or 5 , and an acid labile subunit known as ALS . ^^^ The plasma concentrations of IGF 1 and IGFBP 3 were markedly reduced both in ALS KO mice and in the ALS deficient patients . ^^^
Interacting proteins: P35858 and P17936	Pubmed	SVM Score :0.0
The aims of this study were : ( 1 ) to examine the effect of different therapeutic modalities ( transsphenoidal surgery , TSS ; radiotherapy , RT ; medical treatment with somatostatin analogues , SSA ) on the daily spontaneous GH secretory pattern ( day curve ) ; and ( 2 ) to determine the relationship between the characteristics of the GH secretory pattern and the circulating concentration of IGF 1 , acid labile subunit ( ALS ) and IGFBP 3 . ^^^ IGF 1 , IGFBP 3 and ALS were measured in basal conditions . ^^^ No significant differences were observed with regard to the mean GH , IGF 1 , ALS or IGFBP 3 among the different treatment groups . ^^^
Interacting proteins: P35858 and P17936	Pubmed	SVM Score :0.0
IGF 1 , IGFBP 3 , the acid labile subunit ( ALS ) , N terminal propeptide of type 1 collagen ( PINP ) , C terminal telopeptide of type 1 collagen ( ICTP ) and N terminal propeptide of type 3 collagen ( PIIINP ) were measured in samples collected at baseline ( two samples ) , after 10 , 22 and 24 days of r HuEPO treatment and at the end of the 4 week wash out period . ^^^ There was no significant treatment effect of r HuEPO compared to baseline on IGF 1 , IGFBP 3 , ALS , PINP , ICTP or PIIINP . ^^^
Interacting proteins: P35858 and P17936	Pubmed	SVM Score :0.0
The association of low IGF 1 and ALS with normal IGFBP 3 levels could explain growth impairment of M+ children and could suggest a GH resistance by a late postreceptor signaling defect . . ^^^
Interacting proteins: P35858 and P17936	Pubmed	SVM Score :0.0
However , most of the circulating IGF 1 associates with a high molecular weight complex approximately 150 KDa consisting of IGFBP 3 and the acid labile subunit ( ALS ) ADDIN . ^^^
Interacting proteins: P35858 and P17936	Pubmed	SVM Score :0.0
CONCLUSIONS : ALS is critical for maintaining normal serum concentrations of IGF 1 and IGFBP 3 , most likely by prolonging the half lives of both proteins . ^^^
Interacting proteins: P35858 and P17936	Pubmed	SVM Score :0.0
We report on a 10 yr old Caucasian girl affected by Costello syndrome with fasting hypoglycemia and short stature , associated low circulating levels of acid labile subunit ( ALS ) , relatively low levels of IGF 1 and IGFBP 3 , and normal IGF 2 , mostly circulating in a binary complex with IGFBP 2 and 6 instead of in a 150 kDa ternary complex . ^^^ The reduced ALS concentration and the consequent impaired formation of the circulating 150 kDa ternary complex can induce an accelerated clearance rate of IGF peptides and of IGFBP 3 , contributing to the decreased IGF 1 growth promoting activity in our patient . ^^^
Interacting proteins: P35858 and P17936	Pubmed	SVM Score :0.0
In WT mice , the GH antagonist pegvisomant ( Peg ) suppressed the transcription of endogenous IGF 1 and acid labile subunit ( ALS ) genes with no effect on IGF binding protein 3 ( IGFBP 3 ) mRNA . ^^^ Consequently , Peg induced GH deficiency in WT mice severely reduced ALS , IGF 1 , and IGFBP 3 in the circulation and caused a severe growth deficit . ^^^
Interacting proteins: P35858 and P17936	Pubmed	SVM Score :0.0
The impact of GH deficiency and rhGH replacement therapy on IGF 1 , IGFBP 3 and ALS levels has been widely studied . ^^^ ALS and IGFBP 3 significantly increased ( p < 0 . 001 ) . ^^^ In conclusion , the increase of serum IGF 2 levels during rhGH treatment in GHD patients probably reflects the increase in the other components of ternary complex ( ALS and IGFBP 3 ) . ^^^
Interacting proteins: P35858 and P17936	Pubmed	SVM Score :0.0
IGF 1 circulates predominantly in multi protein complexes consisting of one molecule each of IGF 1 , IGF binding protein 3 and the acid labile subunit ( ALS ) . ^^^
Interacting proteins: P35858 and P17936	Pubmed	SVM Score :0.0
OBJECTIVE : To evaluate the value of measuring plasma insulin like growth factor ( IGF ) 1 and 2 , IGF binding protein 3 ( IGFBP 3 ) and acid labile subunit ( ALS ) as criteria for diagnosing GHD . ^^^ RESULTS : All the patients with certain GHD had low ( < or = 2 z score ) plasma concentrations of IGF 1 and ALS , but only 35 . 1 % had low IGF 2 , and 48 . 6 % had low IGFBP 3 . ^^^ All the patients but three ( 83 . 3 % ) with transient GHD had low IGF 1 , but only 44 . 4 % had low ALS , and only one had low IGF 2 or IGFBP 3 . ^^^
Interacting proteins: P35858 and P17936	Pubmed	SVM Score :0.0
Pregnancy induced changes in insulin like growth factor 1 ( IGF 1 ) , insulin like growth factor binding protein 3 ( IGFBP 3 ) , and acid labile subunit ( ALS ) in patients with growth hormone ( GH ) deficiency and excess . ^^^ BACKGROUND : Under most circumstances with altered growth hormone ( GH ) secretion , the changes of insulin like growth factor 1 ( IGF 1 ) , insulin like growth factor binding protein 3 ( IGFBP 3 ) , and acid labile subunit ( ALS ) are in parallel . ^^^ The aim of the present study was to compare the effects of pregnancy in a hypopituitary patient with those of pregnancy in an acromegalic patient on IGF 1 , IGFBP 3 , and ALS . ^^^
Interacting proteins: P35858 and P17936	Pubmed	SVM Score :0.0
The correlation of the IGF 1 , IGFBP 3 , and ALS generation test to height velocity after 6 months of recombinant growth hormone therapy in girls with Turner syndrome . ^^^ Levels of IGF 1 and IGFBP 3 were measured before and 5 , 30 and 90 days after starting hGH ( 0 . 05 mg / kg / day ) , ALS levels were measured only prior and after 5 days . ^^^ Basal ALS values correlated to height velocity SDS and IGF 1 and IGFBP 3 , after 90 days , correlated to height velocity . ^^^
Interacting proteins: P35858 and P17936	Pubmed	SVM Score :0.0
We genotyped 23 common single nucleotide polymorphisms in IGF 1 , IGFBP 1 , IGFBP 3 and IGFALS , and measured serum levels of IGF 1 and IGFBP 3 in samples of cases and controls . ^^^
Interacting proteins: P35858 and P17936	Pubmed	SVM Score :0.0
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Interacting proteins: P35858 and P17936	Pubmed	SVM Score :0.0
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Interacting proteins: P35858 and P17936	Pubmed	SVM Score :0.0
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Interacting proteins: P35858 and P17936	Pubmed	SVM Score :0.0
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Interacting proteins: P35858 and P17936	Pubmed	SVM Score :0.0
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Interacting proteins: P35858 and P17936	Pubmed	SVM Score :0.0
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Interacting proteins: P35858 and P17936	Pubmed	SVM Score :0.0
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Interacting proteins: P35858 and P17936	Pubmed	SVM Score :0.0
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Interacting proteins: P35858 and P17936	Pubmed	SVM Score :0.0
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Interacting proteins: P35858 and P17936	Pubmed	SVM Score :0.0
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Interacting proteins: P35858 and P17936	Pubmed	SVM Score :0.0
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Interacting proteins: P35858 and P17936	Pubmed	SVM Score :0.0
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Interacting proteins: P35858 and P17936	Pubmed	SVM Score :0.0
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Interacting proteins: P35858 and P17936	Pubmed	SVM Score :0.0
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Interacting proteins: P35858 and P17936	Pubmed	SVM Score :0.0
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Interacting proteins: P35858 and P17936	Pubmed	SVM Score :0.0
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Interacting proteins: P35858 and P17936	Pubmed	SVM Score :0.0
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