| Interacting proteins: P17936 and P19793 |
Pubmed |
SVM Score :0.0 |
| We cloned the nuclear receptor retinoid 10 receptor alpha ( RXR alpha ) as an IGFBP 3 protein partner in a yeast two hybrid screen . ^^^ Multiple methodologies showed that IGFBP 3 and RXR alpha bind each other within the nucleus . ^^^ IGFBP 3 induced apoptosis was abolished in RXR alpha knockout cells . ^^^ Thus , RXR alpha IGFBP 3 interaction leads to modulation of the transcriptional activity of RXR alpha and is essential for mediating the effects of IGFBP 3 on apoptosis . . ^^^ |
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| Interacting proteins: P17936 and P19793 |
Pubmed |
SVM Score :0.0 |
| IGFBP 3 also translocates to the nucleus and has been shown to interact with the nuclear RXRalpha , with evidence that this interaction may mediate its growth inhibitory and proapoptotic effects . ^^^ |
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| Interacting proteins: P17936 and P19793 |
Pubmed |
SVM Score :0.0 |
| The recent discovery of the interaction between nuclear IGFBP 3 and 9 cis retinoic acid receptor alpha ( retinoid 10 receptor alpha RXRalpha ) , a nuclear receptor , and its involvement in the regulation of transcriptional signaling and apoptosis represents an important paradigm shift in the understanding of IGFBP function . ^^^ RXRalpha is required for the apoptosis inducing effects of IGFBP 3 . ^^^ |
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| Interacting proteins: P17936 and P19793 |
Pubmed |
SVM Score :0.0 |
| However , IGFBP 3 binds and modulates the retinoid 10 receptor alpha , interacts with TGFbeta signaling through Smad proteins , and influences other signaling pathways . ^^^ |
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| Interacting proteins: P17936 and P19793 |
Pubmed |
SVM Score :0.0 |
| Since IGFBP 3 is a ligand for the nuclear retinoid 10 receptor alpha , insulin mediated reductions in IGFBP 3 may also influence transcription of anti proliferative genes normally activated by the body ' s endogenous retinoids . ^^^ |
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| Interacting proteins: P17936 and P19793 |
Pubmed |
SVM Score :0.0 |
| Rapid apoptosis induction by IGFBP 3 involves an insulin like growth factor independent nucleomitochondrial translocation of RXRalpha / Nur77 . ^^^ Insulin like growth factor binding protein 3 ( IGFBP 3 ) induces apoptosis by its ability to bind insulin like growth factors ( IGFs ) as well as its IGF independent effects involving binding to other molecules including the retinoid 10 receptor alpha ( RXRalpha ) . ^^^ Here we describe that in response to IGFBP 3 , the RXRalpha binding partner nuclear receptor Nur 77 rapidly undergoes translocation from the nucleus to the mitochondria , initiating an apoptotic cascade resulting in caspase activation within 6 h . ^^^ GFP Nur 77 transfection into RXRalpha wild type and knock out mouse embryonic fibroblasts and subsequent treatment with IGFBP 3 show that RXRalpha is required for IGFBP 3 induced Nur 77 translocation and apoptosis . ^^^ Addition of IGFBP 3 to 22RV1 cell lysates enhanced the ability of GST RXRalpha to `` pull down ' ' Nur 77 , and overexpression of IGFBP 3 enhanced the accumulation of mitochondrial RXRalpha . ^^^ |
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| Interacting proteins: P17936 and P19793 |
Pubmed |
SVM Score :0.0 |
| We have previously identified the retinoid 10 receptor alpha ( RXRalpha ) as an insulin like growth factor binding protein 3 ( IGFBP 3 ) nuclear binding partner , which is required for IGFBP 3 induced apoptosis . ^^^ |
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| Interacting proteins: P17936 and P19793 |
Pubmed |
SVM Score :0.0 |
| A mutant form of IGFBP 3 that does not translocate to the nucleus or bind retinoid 10 receptor alpha was able to inhibit insulin stimulated glucose uptake , indicating that nuclear translocation and retinoid 10 receptor alpha binding are not essential for this IGFBP 3 action . ^^^ |
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| Interacting proteins: P17936 and P19793 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P17936 and P19793 |
Pubmed |
SVM Score :0.0 |
| NA |
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