Pubmed abstracts for Protein-Protein Interaction search result :

Interacting proteins: P36382 and P17302	Pubmed	SVM Score :0.0
The major connexin of the mammalian heart is connexin 43 , although other types of connexins are also expressed , notably connexin 40 in myocytes of the atrioventricular conduction system . ^^^
Interacting proteins: P36382 and P17302	Pubmed	SVM Score :0.0
Equimolar ion substitution of 120 mmol / L KCl for potassium glutamate was used to determine the unitary conductance ratios for rat connexin 40 and connexin 43 , chicken connexin 43 and connexin 45 , and human connexin 37 channels functionally expressed in communication deficient mouse neuroblastoma ( N2A ) cells . ^^^
Interacting proteins: P36382 and P17302	Pubmed	SVM Score :0.0
The principal connexin isoform of the mammalian heart is connexin 43 ; other connexins , notably connexin 40 , connexin 45 and connexin 37 , are also expressed but in smaller quantities . ^^^
Interacting proteins: P36382 and P17302	Pubmed	SVM Score :0.0
Expression of gap junction genes , connexin 40 and connexin 43 , during fetal mouse development . ^^^ The expression patterns of the gap junction genes connexin 40 and connexin 43 have been analyzed during late mouse fetal development , i . e . , at embryonic days 14 . 5 and 16 . 5 , by in situ hybridization and immunofluorescence . ^^^
Interacting proteins: P36382 and P17302	Pubmed	SVM Score :0.0
Three gap junction proteins have been identified in mammalian cardiac myocytes : connexin 43 ( Cx 43 ) , connexin 45 ( Cx 45 ) , and connexin 40 ( Cx 40 ) . ^^^
Interacting proteins: P36382 and P17302	Pubmed	SVM Score :0.0
Connexin 43 and connexin 40 gap junctional proteins are present in arteriolar smooth muscle and endothelium in vivo . ^^^ The distributions of connexin 43 ( Cx 43 ) and connexin 40 ( Cx 40 ) in smooth muscle and endothelium of resistance vessels were examined using indirect immunofluorescence techniques coupled with confocal microscopy . ^^^
Interacting proteins: P36382 and P17302	Pubmed	SVM Score :0.0
Northern blot analysis identified transcripts of connexin 40 ( Cx 40 ) , connexin 43 ( Cx 43 ) , and connexin 45 ( Cx 45 ) genes in all hearts analyzed . ^^^
Interacting proteins: P36382 and P17302	Pubmed	SVM Score :0.0
To determine which connexins were likely to be expressed in the sinus node , atrioventricular ( AV ) node and atrial and ventricular myocardium , messenger ribonucleic acids ( RNAs ) from each of these sites were hybridized with probes for connexin 26 , connexin 31 , connexin 32 , connexin 37 , connexin 40 , connexin 43 , connexin 45 , connexin 46 and connexin 50 . ^^^ Immunostaining with monospecific antibodies to connexin 40 , connexin 43 and connexin 45 was used to delineate the distribution of connexins in frozen sections of these different cardiac tissues . ^^^ Only messenger RNAs coding for connexin 40 , connexin 43 and connexin 45 were detected by Northern blot analysis . ^^^ By immunohistochemical staining , junctions in the sinus and AV nodes and proximal His bundle were virtually devoid of connexin 43 but contained both connexin 40 and connexin 45 . ^^^ Gap junctions in the distal His bundle and the proximal bundle branches stained intensely for connexin 40 and connexin 43 and to a lesser extent for connexin 45 . ^^^
Interacting proteins: P36382 and P17302	Pubmed	SVM Score :0.0
A7r5 cells , a cell line derived from embryonic rat aorta , express connexin 43 ( Cx 43 ) and connexin 40 ( Cx 40 ) . ^^^
Interacting proteins: P36382 and P17302	Pubmed	SVM Score :0.0
Northern blot analysis showed approximately equivalent amounts of mRNAs encoding the gap junction channel proteins connexin 43 and connexin 45 but approximately four times more connexin 40 mRNA in crista terminalis than in the left ventricle . ( ABSTRACT TRUNCATED AT 250 WORDS ) . ^^^
Interacting proteins: P36382 and P17302	Pubmed	SVM Score :0.0
Expression of two connexins , connexin 40 ( Cx 40 ) and connexin 43 ( Cx 43 ) , has been studied in ventricular myocytes from normal and hypertensive rats . ^^^
Interacting proteins: P36382 and P17302	Pubmed	SVM Score :0.0
The spatial distribution and relative abundance of gap junctional connexin 40 and connexin 43 correlate to functional properties of components of the cardiac atrioventricular conduction system . ^^^ Connexin 40 and connexin 43 , the principal gap junctional protein of the mammalian heart , were co localised within atrioventricular nodal tissues and Purkinje fibres . ^^^ By applying a novel photobleach / double labelling protocol , it was demonstrated that connexin 40 and connexin 43 are co localised in precisely the same Purkinje fibre myocytes . ^^^ A model , integrating data on the spatial distribution and relative abundance of connexin 40 and connexin 43 in the heart , proposes how myocyte type specific patterns of connexin isform expression account for the electrical continuity of cardiac atrioventricular conduction . . ^^^
Interacting proteins: P36382 and P17302	Pubmed	SVM Score :0.0
We have recently shown that adult canine ventricular myocytes express three distinct gap junction channel proteins , connexin 40 ( Cx 40 ) , connexin 43 ( Cx 43 ) , and connexin 45 ( Cx 45 ) . ^^^
Interacting proteins: P36382 and P17302	Pubmed	SVM Score :0.0
In addition to the main cardiac gap junction protein connexin 43 ( cx 43 ) , two other constituents , connexin 40 ( cx 40 ) and connexin 45 ( cx 45 ) , recently have been identified . ^^^
Interacting proteins: P36382 and P17302	Pubmed	SVM Score :0.0
Using immunohistochemical staining , the distribution of connexin 40 ( Cx 40 ) and connexin 43 ( Cx 43 ) was studied in rat , guinea pig , porcine , bovine and human hearts . ^^^
Interacting proteins: P36382 and P17302	Pubmed	SVM Score :0.0
Three gap junctional proteins have been identified in canine ventricular myocytes : connexin 43 ( Cx 43 ) , connexin 45 ( Cx 45 ) , and connexin 40 ( Cx 40 ) . ^^^
Interacting proteins: P36382 and P17302	Pubmed	SVM Score :0.0
Colocalization of connexin 43 and connexin 45 but absence of connexin 40 in granulosa cell gap junctions of rat ovary . ^^^
Interacting proteins: P36382 and P17302	Pubmed	SVM Score :0.0
Developmental changes of connexin 40 and connexin 43 mRNA distribution patterns in the rat heart . ^^^ OBJECTIVES : Gap junctions have been demonstrated ultrastructurally in cardiac regions where connexin 40 ( Cx 40 ) and connexin 43 ( Cx 43 ) protein could not be detected immunohistochemically . ^^^
Interacting proteins: P36382 and P17302	Pubmed	SVM Score :0.0
Murine connexin 40 ( Cx 40 ) and connexin 43 ( Cx 43 ) do not form functional heterotypic gap junction channels . ^^^
Interacting proteins: P36382 and P17302	Pubmed	SVM Score :0.0
Whereas endothelial cells of rat aorta and coronary artery characteristically show extensive , prominent connexin 40 , and heterogeneous scattered connexin 37 , the former , unlike the latter , also has abundant connexin 43 . ^^^
Interacting proteins: P36382 and P17302	Pubmed	SVM Score :0.0
Connexin 43 , which comprises gap junctions in astrocytes , was not detectable in pure oligodendrocytic cultures ; mRNAs of connexin 40 and connexin 37 and connexin 26 were also not detected . ^^^
Interacting proteins: P36382 and P17302	Pubmed	SVM Score :0.0
Although independent studies have demonstrated that connexin 43 is abundant in working ventricular myocardium and that connexin 40 is preferentially expressed in the atrioventricular conduction system of a number of species , information on the spatial distribution of connexin 45 in the heart is limited to data obtained using an antibody raised to a single peptide sequence . ^^^
Interacting proteins: P36382 and P17302	Pubmed	SVM Score :0.0
Atrial myocytes express three different gap junction channel proteins connexin 43 ( Cx 43 ) , connexin 45 ( Cx 45 ) , and connexin 40 ( Cx 40 ) whereas ventricular myocytes express only Cx 43 and Cx 45 . ^^^
Interacting proteins: P36382 and P17302	Pubmed	SVM Score :0.0
Double labeling demonstrated that , while some fluorescent spots comprise only connexin 43 , both connexin 40 and connexin 45 predominantly colocalize to connexin 43 positive fluorescent spots . ^^^
Interacting proteins: P36382 and P17302	Pubmed	SVM Score :0.0
Since these responses involve intercellular communication , we have investigated regulation of the gap junction proteins , connexin 26 ( Cx 26 ) , connexin 37 ( Cx 37 ) , connexin 40 ( Cx 40 ) , and connexin 43 ( Cx 43 ) , by mechanical loads . ^^^
Interacting proteins: P36382 and P17302	Pubmed	SVM Score :0.0
Using antibodies for connexin 40 , connexin 43 , connexin 45 , smooth muscle actin , and vimentin , immunocytochemical observations were made with the confocal laser scanning microscope on cryosections of fresh frozen small intestine and colon of the dog and rat . ^^^
Interacting proteins: P36382 and P17302	Pubmed	SVM Score :0.0
Tumour necrosis factor alpha alters the expression of connexin 43 , connexin 40 , and connexin 37 in human umbilical vein endothelial cells . ^^^
Interacting proteins: P36382 and P17302	Pubmed	SVM Score :0.0
To examine the role of cell cell communication via gap junctions in controlling proliferation and differentiation we transfected the malignant trophoblast cell line Jeg 3 , which exhibits extremely low cell cell communication mediated by endogenously expressed connexin 40 , with connexin 26 , connexin 40 , and connexin 43 , respectively . ^^^
Interacting proteins: P36382 and P17302	Pubmed	SVM Score :0.0
Gap junction proteins expressed in the endothelium include connexin 37 , connexin 40 , and connexin 43 . ^^^ Immunoconfocal microscopy showed a high incidence of co localization between connexin 43 and connexin 40 , but lower incidences of co localization between connexin 37 and connexin 40 or connexin 43 . ^^^ Immunoelectron microscopy revealed that 83 % of gap junction profiles contained all three connexins , with the proportion of connexin 40 labeling being significantly higher than that of connexin 37 or connexin 43 . ^^^
Interacting proteins: P36382 and P17302	Pubmed	SVM Score :0.0
To investigate the contribution of gap junction organization and composition to these properties , the spatial pattern of expression of three gap junctional proteins , connexin 45 ( Cx 45 ) , connexin 40 ( Cx 40 ) , and connexin 43 ( Cx 43 ) , was investigated by immunocytochemistry combined with confocal microscopy . ^^^
Interacting proteins: P36382 and P17302	Pubmed	SVM Score :0.0
Some of these are related to the main open state of connexin 43 ( Cx 43 ) ( approximately 100 pS ) , observed between canine ventricular cells , or connexin 40 ( Cx 40 ) ( approximately 215 pS ) observed between transfected N2A cells under similar recording conditions . ^^^
Interacting proteins: P36382 and P17302	Pubmed	SVM Score :0.0
Our laboratory has previously reported that transfection of a connexin 26 ( Cx 26 ) gene , but not connexin 40 nor connexin 43 ( Cx 43 ) , into HeLa cells expressing no detectable level of connexins suppressed the tumorigenic phenotype of the HeLa cells both in vitro and in vivo , although all of these connexins induced gap junctional intercellular communication in HeLa cells to a similar extent . ^^^
Interacting proteins: P36382 and P17302	Pubmed	SVM Score :0.0
Since these responses involve intercellular communication , we have investigated regulation of the gap junction proteins , connexin 43 ( Cx 43 ) , connexin 40 ( Cx 40 ) and connexin 37 ( Cx 37 ) , by cyclical mechanical stretch . ^^^
Interacting proteins: P36382 and P17302	Pubmed	SVM Score :0.0
Distribution of connexin 37 , connexin 40 and connexin 43 in the aorta and coronary artery of several mammals . ^^^
Interacting proteins: P36382 and P17302	Pubmed	SVM Score :0.0
Endothelial cells express three different connexins ( connexin 37 , connexin 40 , and connexin 43 ) in vivo . ^^^ To examine the potential ability of these connexins to form heteromeric channels ( containing different connexins within the same hemi channel ) , we stably transfected connexin 43 containing normal rat kidney ( NRK ) cells with connexin 37 or connexin 40 . ^^^
Interacting proteins: P36382 and P17302	Pubmed	SVM Score :0.0
RESULTS : Connexin 40 immunostaining was not detected in ventricular cardiomyocytes in any animal group but connexin 43 was found in all groups . ^^^
Interacting proteins: P36382 and P17302	Pubmed	SVM Score :0.0
Endothelial cells form gap junctions that , according to vessel type , may be composed of up to 3 types of connexin , connexin 37 , connexin 40 , and connexin 43 . ^^^ Gap junction spots labeled by specific antisera against connexin 37 , connexin 40 , and connexin 43 were quantified 7 , 14 , and 28 days after injury , and the relations among the connexins were examined by using colocalization analysis . ^^^ Although connexin 40 gap junctional spot size and area returned to uninjured levels by 28 days after injury , connexin 37 and connexin 43 spot size and area exceeded those of the uninjured artery ( P < 0 . 05 ) . ^^^
Interacting proteins: P36382 and P17302	Pubmed	SVM Score :0.0
The expression of connexin 43 protein was confirmed , but connexin 40 protein was not detected by immunocytochemistry or immunoblot analysis . ^^^
Interacting proteins: P36382 and P17302	Pubmed	SVM Score :0.0
Abnormal cardiac conduction and morphogenesis in connexin 40 and connexin 43 double deficient mice . ^^^ Connexin 40 deficient ( Cx 40 ( / ) / Cx43 ( + / + ) ) and connexin 43 heterozygous knockout mice ( Cx 40 ( + / + ) / Cx43 ( + / ) ) are viable but show cardiac conduction abnormalities . ^^^
Interacting proteins: P36382 and P17302	Pubmed	SVM Score :0.0
In the heart , spatially defined patterns of expression of three connexin isotypes connexin 43 , connexin 40 , and connexin 45 underlie the precisely orchestrated patterns of current flow governing the normal cardiac rhythm . ^^^ Derangement of gap junction organization and / or reduced expression of connexin 43 are associated with arrhythmic tendency in the diseased human ventricle , and high levels of connexin 40 in the atrium are associated with increased risk of developing atrial fibrillation after coronary by pass surgery . ^^^ In the major arteries , endothelial gap junctions may simultaneously express three connexin isotypes , connexin 40 , connexin 37 , and connexin 43 ; underlying medial smooth muscle , by contrast , predominantly expresses connexin 43 , with connexin 45 additionally expressed at restricted sites . ^^^
Interacting proteins: P36382 and P17302	Pubmed	SVM Score :0.0
These transgenic animals exhibit decreased systolic function and inward 1 ( Ca , L ) , low levels of connexin 43 and connexin 40 , sinus bradycardia , and prolonged atrioventricular ( AV ) node conduction followed by complete heart block and sudden death . ^^^
Interacting proteins: P36382 and P17302	Pubmed	SVM Score :0.0
While immunoreactivity to connexin 40 was strongly expressed on the membranes of endothelial cells only , that to connexin 43 was expressed on both smooth muscle and endothelial cell membranes . 4 . ^^^
Interacting proteins: P36382 and P17302	Pubmed	SVM Score :0.0
Gap junction channels formed by coexpressed connexin 40 and connexin 43 . ^^^
Interacting proteins: P36382 and P17302	Pubmed	SVM Score :0.0
In this study , we examined the effects of ET 1 and AT 2 on connexin 43 ( Cx 43 ) and connexin 40 ( Cx 40 ) in cultured neonatal rat ventricular cardiomyocytes ( NRCs ) and the role of mitogen activated protein kinase signaling in the ET 1 and AT 2 induced responses . ^^^
Interacting proteins: P36382 and P17302	Pubmed	SVM Score :0.0
Recent evidence indicating formation of functional homomeric / heterotypic gap junction channels by connexin 40 ( Cx 40 ) and connexin 43 ( Cx 43 ) raises the question of whether data previously interpreted as support for heteromeric channel formation by these connexins might not instead reflect the activity of homomeric / heterotypic channels . ^^^
Interacting proteins: P36382 and P17302	Pubmed	SVM Score :0.0
Sinoatrial node cells with a projection area between 800 and 1200 microm ( 2 ) had a lower amount of connexin 45 label and again a small amount of connexin 40 but an increased amount of connexin 43 label . ^^^
Interacting proteins: P36382 and P17302	Pubmed	SVM Score :0.0
This study tested the hypothesis that the quantity of immunodetectable atrial gap junctional proteins , connexin 40 ( Cx 40 ) and connexin 43 ( Cx 43 ) , are related to atrial conduction velocity in humans . ^^^
Interacting proteins: P36382 and P17302	Pubmed	SVM Score :0.0
RESULTS : 43Gap 27 , a peptide homologous to the second extracellular loop of connexin 43 , partially inhibited the L NAME and indomethacin resistant RBF response to acetylcholine , whereas 40Gap 27 , homologous to the second extracellular loop of connexin 40 , abolished the response . ^^^
Interacting proteins: P36382 and P17302	Pubmed	SVM Score :0.0
Connexin 40 ( Cx 40 ) , connexin 37 ( Cx 37 ) and connexin 43 ( Cx 43 ) are subunit proteins of gap junction channels in the vascular wall which are presumably involved in the propagation of vasomotor signals . ^^^
Interacting proteins: P36382 and P17302	Pubmed	SVM Score :0.0
OBJECTIVES : To elucidate the structural basis for the electrophysiologic remodeling induced by chronic atrial fibrillation ( AF ) , we investigated connexin 40 and connexin 43 ( Cx 40 and Cx 43 ) expression and distribution in atria of patients with and without chronic AF and in an animal model of AF with additional electrophysiologic investigation of anisotropy ( ratio of longitudinal and transverse velocities ) . ^^^
Interacting proteins: P36382 and P17302	Pubmed	SVM Score :0.0
Spatially defined patterns of expression of three connexin isoforms connexin 43 , connexin 40 and connexin 45 form the cell to cell conduction pathways responsible for the orderly spread of current flow that governs the normal cardiac rhythm . ^^^
Interacting proteins: P36382 and P17302	Pubmed	SVM Score :0.0
Several proteins including connexin 40 ( Cx 40 ) and connexin 43 ( Cx 43 ) form gap junctions between cells of the heart ; they may be found separately or may be coexpressed . ^^^
Interacting proteins: P36382 and P17302	Pubmed	SVM Score :0.0
Treatments that induced dye coupling also induced connexin 43 and connexin 40 , but not connexin 32 immunoreactivity . ^^^
Interacting proteins: P36382 and P17302	Pubmed	SVM Score :0.0
Gap junction protein distribution was examined using antibodies against connexin 40 ( Cx 40 ) and connexin 43 ( Cx 43 ) . ^^^
Interacting proteins: P36382 and P17302	Pubmed	SVM Score :0.0
The most widespread is connexin 43 , but connexin 40 is also present in secondary lymphoid organs . ^^^
Interacting proteins: P36382 and P17302	Pubmed	SVM Score :0.0
Spatially defined patterns of expression of 3 connexin isotypes connexin 43 , connexin 40 , and connexin 45 form specific cell to cell conduction pathways for the spread of current flow that governs the normal cardiac rhythm . ^^^
Interacting proteins: P36382 and P17302	Pubmed	SVM Score :0.0
Pharmacological modulation and differential regulation of the cardiac gap junction proteins connexin 43 and connexin 40 . ^^^ Cardiac gap junction channels are predominantly composed of connexin 40 or connexin 43 . ^^^
Interacting proteins: P36382 and P17302	Pubmed	SVM Score :0.0
Specific antibodies were used to delineate the distribution of connexin 37 , connexin 40 , connexin 43 , and connexin 45 within the arterial wall . ^^^ Gap junction plaques , whose incidence was highest in endothelium , were constructed from connexin 40 and connexin 43 in the media and connexin 37 , connexin 40 , and connexin 43 in the endothelium . ^^^
Interacting proteins: P36382 and P17302	Pubmed	SVM Score :0.0
Immunocytochemistry showed that fibroblasts and cardiomyocytes expressed connexin 43 and connexin 45 , whereas connexin 40 was absent . ^^^
Interacting proteins: P36382 and P17302	Pubmed	SVM Score :0.0
The gap junction proteins connexin 40 and connexin 43 were downregulated in the transgenic hearts , indicating that ACE 2 mediated gap junction remodeling may account for the observed electrophysiologic disturbances . ^^^
Interacting proteins: P36382 and P17302	Pubmed	SVM Score :0.0
Selective effect of PDGF on connexin 43 versus connexin 40 comprised gap junction channels . ^^^
Interacting proteins: P36382 and P17302	Pubmed	SVM Score :0.0
Of the 20 connexins in vertebrates , four are expressed in developing heart : connexin 37 ( Cx 37 ) , connexin 40 ( Cx 40 ) , connexin 43 ( Cx 43 ) , and connexin 45 ( Cx 45 ) . ^^^
Interacting proteins: P36382 and P17302	Pubmed	SVM Score :0.0
INTRODUCTION : Gap junctional connexin proteins ( connexin 40 [ Cx 40 ] , connexin 43 [ Cx 43 ] ) are a determinant of myocardial conduction and are implicated in the development of atrial fibrillation ( AF ) . ^^^
Interacting proteins: P36382 and P17302	Pubmed	SVM Score :0.0
Different combinations and relative quantities of three connexins connexin 43 , connexin 40 and connexin 45 are expressed in different subsets of cardiomyocyte . ^^^
Interacting proteins: P36382 and P17302	Pubmed	SVM Score :0.0
OBJECTIVE : To investigate the relationship between the alternations in the expression and distribution of connexin 40 ( Cx 40 ) and connexin 43 ( Cx 43 ) and atrial fibrillation ( AF ) in patients with rheumatic heart disease . ^^^
Interacting proteins: P36382 and P17302	Pubmed	SVM Score :0.0
Rapid spreading of the electrical impulse throughout the heart is essential for coordinated contraction and is mediated by electrical coupling of cardiomyocytes through gap junction channels composed of connexin 40 ( Cx 40 ) , connexin 43 ( Cx 43 ) or connexin 45 ( Cx 45 ) . ^^^
Interacting proteins: P36382 and P17302	Pubmed	SVM Score :0.0
As in most tissues and organs , multiple connexin types are expressed in the heart ; connexin 43 , connexin 40 and connexin 45 are found in distinctive combinations and relative quantities in different , functionally specialized subsets of cardiomyocyte . ^^^
Interacting proteins: P36382 and P17302	Pubmed	SVM Score :0.0
Connexin 40 ( Cx 40 ) contains a specific binding site for spermine ( affinity approximately 100 microm ) whereas connexin 43 ( Cx 43 ) is unaffected by identical concentrations of intracellular spermine . ^^^
Interacting proteins: P36382 and P17302	Pubmed	SVM Score :0.0
Whole mount immunohistochemistry for connexins revealed punctate labeling at borders of arteriolar endothelial cells , with connexin 40 and connexin 37 in all branches and connexin 43 only in the largest branches . ^^^
Interacting proteins: P36382 and P17302	Pubmed	SVM Score :0.0
The inhibition by the connexin 43 inhibitor was greater than that of the connexin 40 inhibitor . ^^^
Interacting proteins: P36382 and P17302	Pubmed	SVM Score :0.0
METHODS : Protein concentrations of connexin 40 and connexin 43 were analysed in left atrial tissue of patients undergoing cardiac surgery . ^^^ RESULTS : Western blot analysis of connexin 40 and connexin 43 expression showed an increase of both gap junctional proteins ( connexin 43 > connexin 40 ) in patients with AF of all forms compared with patients in sinus rhythm ( p = 0 . 01 and p = 0 . 011 , respectively ) . ^^^ Subgroup analysis showed increased concentrations of connexin 40 in lone AF and AF with mitral valve disease compared with sinus rhythm ( p = 0 . 06 and p = 0 . 029 , respectively ) , whereas the same analysis for connexin 43 reached significance only in the mitral valve disease group ( p = 0 . 031 ) . ^^^ No differences in connexin 40 and connexin 43 expression were detectable between lone AF and AF with mitral valve disease . ^^^ Within the groups connexin 40 and connexin 43 expression did not differ between patients with paroxysmal AF and patients with chronic AF . ^^^
Interacting proteins: P36382 and P17302	Pubmed	SVM Score :0.0
Statins reduce connexin 40 and connexin 43 expression in atherosclerotic aorta of rabbits . ^^^
Interacting proteins: P36382 and P17302	Pubmed	SVM Score :0.0
Chronic regulation of the expression of gap junction proteins connexin 40 , connexin 43 , and connexin 45 in neonatal rat cardiomyocytes . ^^^ In the working myocardium , the connexin 43 ( Cx 43 ) is most abundantly found , whereas connexin 40 ( Cx 40 ) is expressed in the atria and in the conduction system [ together with low levels of connexin 45 ( Cx 45 ) ] . ^^^ Inasmuch as TNFalpha may play a contributory role in the concert of factors involved in the pathophysiology of heart failure and because this cardiac disease often leads to ventricular reentrant arrhythmia , the goal of our study was to find out whether TNFalpha may influence the expression of the cardiac connexins connexin 43 , connexin 40 , and connexin 45 . ^^^ Connexin 43 , connexin 40 , and connexin 45 expressions were analysed using Western blot analysis , immunohistology , and polymerase chain reaction ( PCR ) studies ( connexin 43 and connexin 40 ) . ^^^ TNFalpha induced a concentration dependent increase in connexin 43 ( by 2 . 9+ / 0 . 6 , P < 0 . 05 , n=5 ) but not in connexin 40 or connexin 45 expressions . ^^^
Interacting proteins: P36382 and P17302	Pubmed	SVM Score :0.0
Conduction defects were correlated with dephosphorylation of connexin 40 and connexin 43 and upregulation of protein phosphatase 1beta ( PP1beta ) . ^^^
Interacting proteins: P36382 and P17302	Pubmed	SVM Score :0.0
Connexin 40 and connexin 43 expression was unchanged . ^^^
Interacting proteins: P36382 and P17302	Pubmed	SVM Score :0.0
In addition , ovariectomy decreased the EDHF mediated component of the relaxation and membrane hyperpolarization of the smooth muscle cells , an effect which might be explained by a concomitant decrease of the expression of the gap junction connexin 40 and connexin 43 . ^^^
Interacting proteins: P36382 and P17302	Pubmed	SVM Score :0.0
Connexin 40 and connexin 43 in mouse aortic endothelium : evidence for coordinated regulation . ^^^ To reveal a possible interaction between connexin 40 ( Cx 40 ) and connexin 43 ( Cx 43 ) in endothelium , we studied their distribution in vessels from C57Bl / 6 and Cx 40 knockout mice ( Cx 40 / ) using immunoblots and immunocytochemistry on aortic cross sections and en face whole mounts . ^^^
Interacting proteins: P36382 and P17302	Pubmed	SVM Score :0.0
Expression of connexin 40 and connexin 43 was not significantly changed by ANG 2 as assessed by immunohistochemistry and Western blot analysis . ^^^
Interacting proteins: P36382 and P17302	Pubmed	SVM Score :0.0
Clones were homogeneous morphologically , and expressed transcription factors ( GATA 4 , MYCD , NFATc 4 ) , contractile proteins such as alpha and beta myosin heavy chain , alpha cardiac actin , troponin 1 , desmoplakin , alpha actinin , the muscle specific intermediate filament protein , desmin , the cardiomyocyte specific peptide hormones , BNP , the L type calcium channel alpha1C subunit and gap junction proteins , connexin 43 and connexin 40 . ^^^
Interacting proteins: P36382 and P17302	Pubmed	SVM Score :0.0
Immunoexpression of HNK 1 , connexin 40 , and connexin 43 were examined in serial sections . ^^^
Interacting proteins: P36382 and P17302	Pubmed	SVM Score :0.0
By immunofluorescence and western analysis , the transcription factor Hf1b / Sp4 and the gap junction proteins connexin 40 and connexin 43 were misexpressed and / or mislocalized in LmnaN195K / N195K hearts . ^^^
Interacting proteins: P36382 and P17302	Pubmed	SVM Score :0.0
A significant decrease in the gap junction proteins , connexin 43 and connexin 40 , was observed in N cadherin depleted myocytes . ^^^
Interacting proteins: P36382 and P17302	Pubmed	SVM Score :0.0
In diabetes , connexin 40 was expressed in smooth muscle cells along afferent arterioles , glomerular connexin staining was more extensive and connexin 43 was detected in renin secreting cells . ^^^
Interacting proteins: P36382 and P17302	Pubmed	SVM Score :0.0
The most important cardiac gap junction proteins are connexin 43 ( predominantly ) ( Cx 43 ) , connexin 40 ( Cx 40 ) , and in early developmental stages connexin 45 . ^^^
Interacting proteins: P36382 and P17302	Pubmed	SVM Score :0.0
This is in contrast to previous results from our laboratory which showed that replacement of connexin 43 by connexin 40 resulted in morphologically and functionally normal hearts . ^^^
Interacting proteins: P36382 and P17302	Pubmed	SVM Score :0.0
Myocardial dysfunction and arrhythmias may be induced by congenital heart defects , but also be the result of heart surgery with cardiopulmonary bypass ( CPB ) , potentially caused by differential expression of connexin 40 ( Cx 40 ) and connexin 43 ( Cx 43 ) . ^^^
Interacting proteins: P36382 and P17302	Pubmed	SVM Score :0.0
Additionally , neither connexin 40 nor connexin 45 , but only connexin 43 was detected between bilayer cardiomyocyte sheets within 30 min after layering . ^^^
Interacting proteins: P36382 and P17302	Pubmed	SVM Score :0.0
METHODS AND RESULTS : The rat AVN was identified by Masson ' s trichrome staining together with immunolabeling of marker proteins : connexin 40 , connexin 43 , desmoplakin , atrial natriuretic peptide , and hyperpolarization activated and cyclic nucleotide gated channel 4 . ^^^
Interacting proteins: P36382 and P17302	Pubmed	SVM Score :0.0
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Interacting proteins: P36382 and P17302	Pubmed	SVM Score :0.0
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Interacting proteins: P36382 and P17302	Pubmed	SVM Score :0.0
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Interacting proteins: P36382 and P17302	Pubmed	SVM Score :0.0
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Interacting proteins: P36382 and P17302	Pubmed	SVM Score :0.0
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Interacting proteins: P36382 and P17302	Pubmed	SVM Score :0.0
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Interacting proteins: P36382 and P17302	Pubmed	SVM Score :0.0
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Interacting proteins: P36382 and P17302	Pubmed	SVM Score :0.0
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Interacting proteins: P36382 and P17302	Pubmed	SVM Score :0.0
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Interacting proteins: P36382 and P17302	Pubmed	SVM Score :0.0
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Interacting proteins: P36382 and P17302	Pubmed	SVM Score :0.0
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Interacting proteins: P36382 and P17302	Pubmed	SVM Score :0.0
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Interacting proteins: P36382 and P17302	Pubmed	SVM Score :0.0
NA
Interacting proteins: P36382 and P17302	Pubmed	SVM Score :0.0
NA
Interacting proteins: P36382 and P17302	Pubmed	SVM Score :0.0
NA