| Interacting proteins: P15407 and P17275 |
Pubmed |
SVM Score :0.0 |
| ET isopeptides induced a subset of fos and jun mRNAs in mesangial cells , including c fos , fra 1 , c jun , and JunB . fos and jun mRNAs were induced as members of the immediate early gene response . ^^^ |
|
| Interacting proteins: P15407 and P17275 |
Pubmed |
SVM Score :0.0 |
| We have shown that while c Fos is the major Fos protein associated with the Jun proteins ( c Jun , JunB , and JunD ) soon after serum stimulation , at later times Fra 1 and Fra 2 are the predominant Fos proteins associated with the different Jun proteins . ^^^ |
|
| Interacting proteins: P15407 and P17275 |
Pubmed |
SVM Score :0.0 |
| Northern analysis indicates that mRNAs for at least six members of the Fos / Jun family ( c fos , fosB , fra 1 , c jun , junB , and junD ) are expressed in activated Ar 5 cells ; thus the AP 1 sites of the IL 2 promoter may bind different dimeric Fos / Jun complexes at different times after T cell activation , perhaps mediating both positive and negative regulation of the IL 2 promoter . . ^^^ |
|
| Interacting proteins: P15407 and P17275 |
Pubmed |
SVM Score :0.0 |
| Okadaic acid treatment was found to dramatically increase mRNA transcripts of the jun family of proto oncogenes including c jun , junD , and junB and to a lesser extent the fos family including c fos and fra 1 . ^^^ |
|
| Interacting proteins: P15407 and P17275 |
Pubmed |
SVM Score :0.0 |
| Consistent with this result , these cell lines expressed increased levels of mRNAs encoding the AP 1 proteins , c Fos , Fra 1 , c Jun , JunB , and JunD . ^^^ |
|
| Interacting proteins: P15407 and P17275 |
Pubmed |
SVM Score :0.0 |
| To determine whether these proteins are required for cell cycle progression , we microinjected affinity purified antibodies directed against c Fos , FosB , Fra 1 , c Jun , JunB , and JunD , and antibodies that recognize either the Fos or the Jun family of proteins , into Swiss 3T3 cells and determined their effects in cell cycle progression by monitoring DNA synthesis . ^^^ |
|
| Interacting proteins: P15407 and P17275 |
Pubmed |
SVM Score :0.0 |
| Like c Fos and Fra 1 , Fra 2 formed stable heterodimers with c Jun , JunB or JunD in vitro and all these complexes had specific DNA binding activity to AP 1 binding sites ( AP 1 sites ) or related sequences . ^^^ |
|
| Interacting proteins: P15407 and P17275 |
Pubmed |
SVM Score :0.0 |
| Likewise , expression of fra 1 , c jun and junB continued to be high in serum stimulated senescent cells , while induction of fosB was reduced approximately fivefold . ^^^ |
|
| Interacting proteins: P15407 and P17275 |
Pubmed |
SVM Score :0.0 |
| Fos related antigen ( Fra 1 ) , junB , and junD activate human involucrin promoter transcription by binding to proximal and distal AP 1 sites to mediate phorbol ester effects on promoter activity . ^^^ Gel supershift experiments indicate the interaction of these sites with Fra 1 , junB , and junD . ^^^ We conclude that ( 1 ) two AP 1 sites in the hINV promoter are important elements required for keratinocyte specific expression , ( 2 ) these AP 1 1 sites mediate the phorbol ester dependent increase in promoter activity , and ( 3 ) Fra 1 , junB , and junD may be important regulators of hINV expression in epidermis . . ^^^ |
|
| Interacting proteins: P15407 and P17275 |
Pubmed |
SVM Score :0.0 |
| BHQ induced expression of c jun , junB , fra 1 , and fra 2 , which encode AP 1 components , but was a poor inducer of c fos and had no effect on fosB . ^^^ |
|
| Interacting proteins: P15407 and P17275 |
Pubmed |
SVM Score :0.0 |
| Although several members of the AP 1 family were able to activate the PLF gene promoter in transient cotransfection experiments , the predominant AP 1 components interacting with the PLF gene promoter in serum stimulated cells were Fra 1 , JunB , and JunD . ^^^ |
|
| Interacting proteins: P15407 and P17275 |
Pubmed |
SVM Score :0.0 |
| Immediate early gene expression was examined by Northern blotting : both thrombin and PDGF induced egr 1 , c fos , c jun , junB , and fra 1 mRNAs within 15 min ; the response was maximal at 30 60 min ( increases ranging from 2 . 9 to 9 . 3 fold over control serum deprived cells ) and returned to base line levels within 2 4 h . ^^^ |
|
| Interacting proteins: P15407 and P17275 |
Pubmed |
SVM Score :0.0 |
| Using a seasonal breeder , the European red fox ( Vulpes vulpes ) , as our model , we have examined the expression of five AP 1 family members ( c fos , fra 1 , fra 2 , c jun and junB ) with a view to elucidating their role in the regulation of spermatogenesis . ^^^ Unique patterns of expression , falling into three broad categories , were observed for the five genes : ( 1 ) continuous expression throughout the spermatogenic cycle ( c fos ) ; ( 2 ) expression only at times corresponding to the onset and shutdown of spermatogenesis ( fra 1 , fra 2 and c jun ) ; and ( 3 ) expression only at the onset of the cycle ( junB ) . ^^^ |
|
| Interacting proteins: P15407 and P17275 |
Pubmed |
SVM Score :0.0 |
| The NFAT 1 DNA binding complex in activated T cells contains Fra 1 and JunB . ^^^ These experiments demonstrated that the NFAT 1 binding complex contains JunB and Fra 1 proteins . ^^^ Northern ( RNA ) blot analyses demonstrate that both fra 1 and junB mRNAs are induced upon T cell activation , although fra 1 mRNA is present even in quiescent T cells . ^^^ Taken together , these results suggested that the JunB Fra 1 heterodimer is the inducible nuclear component of the NFAT 1 binding activity and that JunB expression regulates the formation of the heterodimer . ^^^ |
|
| Interacting proteins: P15407 and P17275 |
Pubmed |
SVM Score :0.0 |
| Using in situ hybridization , the expression of c fos , fosB , fra 1 , fra 2 , c jun and junB was studied after 15 min of normothermic and hypothermic ( 33 degrees C ) transient forebrain ischaemia in the rat , induced by common carotid occlusion combined with systemic hypotension . ^^^ |
|
| Interacting proteins: P15407 and P17275 |
Pubmed |
SVM Score :0.0 |
| Differential expression of the fos and jun family members c fos , fosB , Fra 1 , Fra 2 , c jun , junB and junD during human epidermal keratinocyte differentiation . ^^^ In the present study we describe the localization of c fos , fosB , Fra 1 , Fra 2 , c jun , junB and junD in the normal human epidermis . ^^^ |
|
| Interacting proteins: P15407 and P17275 |
Pubmed |
SVM Score :0.0 |
| Calcium induced AP 1 DNA binding complexes consist of Fra 1 , Fra 2 , c Jun , JunB and JunD and are independent of c Fos , since the induction of DNA binding activity and the composition of the AP 1 binding complexes are identical in differentiating keratinocytes derived from c fos null and wild type mice . ^^^ In vivo expression patterns suggest that the predominant AP 1 heterodimer in the granular layer consists of Fra 2 and JunB while a JunD and Fra 1 complex predominates the spinous layer of mouse epidermis . ^^^ |
|
| Interacting proteins: P15407 and P17275 |
Pubmed |
SVM Score :0.0 |
| Because both fra 1 and junB overexpression negatively interferes with c jun / c fos trans activation of AP 1 responsive genes , our results suggest that the observed block in viral transcription is mainly the consequence of an antioxidant induced reconstitution of the AP 1 transcription complex . ^^^ |
|
| Interacting proteins: P15407 and P17275 |
Pubmed |
SVM Score :0.0 |
| In the endothelial cells , all Jun and Fos forms ( c Jun , JunB , JunD , c Fos , FosB , Fra 1 and Fra 2 ) were part of the AP 1 complex after PMA induction . ^^^ |
|
| Interacting proteins: P15407 and P17275 |
Pubmed |
SVM Score :0.0 |
| These transformed fibroblasts accumulated higher levels of cJun , JunB , Fra 1 and Fra 2 proteins relative to their normal counterparts . ^^^ |
|
| Interacting proteins: P15407 and P17275 |
Pubmed |
SVM Score :0.0 |
| The GR AP 1 site bound protein complexes composed of JunD , JunB , Fra 2 , and Fra 1 from L 929 nuclear extracts . ^^^ |
|
| Interacting proteins: P15407 and P17275 |
Pubmed |
SVM Score :0.0 |
| Neoplastic transformation of rat thyroid cells requires the junB and fra 1 gene induction which is dependent on the HMGI C gene product . ^^^ The strongest effect is represented by the dramatic junB and fra 1 gene induction , which is prevented in cell lines expressing the antisense HMGI C . ^^^ These results indicate that the HMGI C gene product is essential for the junB and fra 1 transcriptional induction associated with neoplastic transformation . ^^^ |
|
| Interacting proteins: P15407 and P17275 |
Pubmed |
SVM Score :0.0 |
| In both cell types , c Fos and Fra 1 proteins increased after EGF treatment , but differences in the induction of Jun proteins were noted , with an increase of c Jun in hepatocytes and an increase of JunB in 7777 cells . ^^^ |
|
| Interacting proteins: P15407 and P17275 |
Pubmed |
SVM Score :0.0 |
| In the present study , overexpression of fra 1 , fra 2 , c jun , junB , and junD mRNAs was demonstrated in RCs by Northern blot analysis . ^^^ |
|
| Interacting proteins: P15407 and P17275 |
Pubmed |
SVM Score :0.0 |
| We have investigated the effect of a 30 min running bout in untrained subjects on the expression of the mRNAs of all members of the fos and jun gene families , including c fos , fosB , fosBdel , fra 1 , and fra 2 as well as c jun , junB , and junD . ^^^ |
|
| Interacting proteins: P15407 and P17275 |
Pubmed |
SVM Score :0.0 |
| We found a rapid induction of c fos , c jun and junB at the mRNA level after about 30 min of EGF treatment and a more delayed upregulation of fosB and fra 1 . ^^^ |
|
| Interacting proteins: P15407 and P17275 |
Pubmed |
SVM Score :0.0 |
| The delayed increase in COX 2 mRNA expression was accompanied by the induction of the proto oncogenes c jun , junB , junD , and c fos ( but not FosB or Fra 1 ) . ^^^ |
|
| Interacting proteins: P15407 and P17275 |
Pubmed |
SVM Score :0.0 |
| Expression of c jun , junB , c fos , fra 1 and fra 2 mRNA in the rat brain following seizure activity and axotomy . ^^^ The patterns of c jun , junB , c fos , fra 1 and fra 2 mRNAs were studied by radioactive and non radioactive in situ hybridization in the adult rat brain following kainate induced seizure activity and axotomy . ^^^ Whereas fra 1 and fra 2 were restricted to the hippocampus , c jun , junB and c fos were additionally induced in the cortex , amygdala and thalamus . ^^^ |
|
| Interacting proteins: P15407 and P17275 |
Pubmed |
SVM Score :0.0 |
| This finding is paralleled by the observation that among the various members of the Fos and Jun family analysed ( c Fos , FosB , Fra 1 , Fra 2 , c Jun , JunD , JunB ) fra 1 is the only gene to be exclusively expressed in NSCLC cells but not in cells of SCLC origin . ^^^ |
|
| Interacting proteins: P15407 and P17275 |
Pubmed |
SVM Score :0.0 |
| The concentration of c fos , c jun , c myc , junB , and fra 1 mRNAs was increased in activated peripheral blood lymphocytes incubated with ciprofloxacin compared to that in untreated controls . ^^^ |
|
| Interacting proteins: P15407 and P17275 |
Pubmed |
SVM Score :0.0 |
| The most important effects were represented by the dramatic junB and fra 1 gene induction , which was abolished by the block of the transformation induced HMGI C protein synthesis . ^^^ A significant increase of Fra 1 and JunB protein levels was detected in all oncogene transformed rat thyroid cell lines . ^^^ |
|
| Interacting proteins: P15407 and P17275 |
Pubmed |
SVM Score :0.0 |
| The activator protein 1 ( AP 1 ) transcriptional complex is made up of members of the Fos ( c Fos , FosB , Fra 1 , Fra 2 ) and Jun ( c Jun , JunB , JunD ) families and is stimulated by insulin in several cell types . ^^^ In the current study we show that the AP 1 complex isolated from insulin stimulated cells contained c Fos , Fra 1 , c Jun and JunB . ^^^ |
|
| Interacting proteins: P15407 and P17275 |
Pubmed |
SVM Score :0.0 |
| We have examined the expression of members of the AP 1 transcription factor complex in response to stimulation with different doses of LPA . c Fos , c Jun , and JunB are induced rapidly in response to LPA stimulation , whereas Fra 1 and Fra 2 are induced after a significant lag . ^^^ The expression of c Fos is transient , whereas the expression of c Jun , JunB , Fra 1 , and Fra 2 is sustained . ^^^ In contrast , expression of Fra 1 , Fra 2 , and JunB and optimal expression of c Jun are observed only with doses of LPA which induce sustained MAPK activation and DNA synthesis . ^^^ LPA stimulated expression of c Fos , Fra 1 , Fra 2 , c Jun , and JunB is inhibited by the MEK 1 inhibitor PD 098059 , indicating that the Raf MEK MAPK cascade is required for their expression . ^^^ In cells expressing a conditionally active form of Raf 1 ( DeltaRaf 1 : ER ) , we observed that selective , sustained activation of Raf MEK MAPK was sufficient to induce expression of Fra 1 , Fra 2 , and JunB but , interestingly , induced little or no c Fos or c Jun . ^^^ |
|
| Interacting proteins: P15407 and P17275 |
Pubmed |
SVM Score :0.0 |
| This article reviews findings up to the end of 1997 about the inducible transcription factors ( ITFs ) c Jun , JunB , JunD , c Fos , FosB , Fra 1 , Fra 2 , Krox 20 ( Egr 2 ) and Krox 24 ( NGFI A , Egr 1 , Zif 268 ) ; and the constitutive transcription factors ( CTFs ) CREB , CREM , ATF 2 and SRF as they pertain to gene expression in the mammalian nervous system . ^^^ |
|
| Interacting proteins: P15407 and P17275 |
Pubmed |
SVM Score :0.0 |
| Supershift analysis identified CREB 1 , JunB , c Fos , Fra 1 , and c Jun in protein complexes isolated from differentiated rabbit tracheal epithelial cells binding to this site . ^^^ |
|
| Interacting proteins: P15407 and P17275 |
Pubmed |
SVM Score :0.0 |
| Supershift analysis of AP 1 / DNA complexes with antibodies against the AP 1 sub units , c Fos , FosB , Fra 1 , Fra 2 , c Jun , JunB , and JunD , revealed that the AP 1 / DNA complexes in the various clones had different compositions . ^^^ |
|
| Interacting proteins: P15407 and P17275 |
Pubmed |
SVM Score :0.0 |
| Supershift assays revealed that fra 1 participates in the activator protein 1 complex together with c fos , c jun and junB . ^^^ |
|
| Interacting proteins: P15407 and P17275 |
Pubmed |
SVM Score :0.0 |
| Thrombin stimulated a rapid but transient accumulation of c Fos whereas the expression of Fra 1 , Fra 2 , c Jun and JunB was sustained throughout the G 1 phase of the cell cycle . ^^^ We focussed our analysis on c Fos ( typical of AP 1 genes which are expressed rapidly and transiently ) and Fra 1 and JunB ( typical of AP 1 genes expressed after a delay but in a sustained manner ) . ^^^ The expression of c Fos , Fra 1 and JunB was dependent upon the activation of MAPK since these responses were inhibited by PD 098059 . ^^^ However , a comparison of responses to FBS , thrombin , TRPs , LPA and EGF revealed that Fra 1 and JunB expression required sustained activation of MAPK whereas c Fos expression was strongly induced even by non mitogenic stimuli which elicited only transient MAPK activation . ^^^ The expression of c Fos ( in response to thrombin , TRP or LPA ) or Fra 1 , JunB and cyclin D 1 ( thrombin only ) was also inhibited by pertussis toxin . ^^^ |
|
| Interacting proteins: P15407 and P17275 |
Pubmed |
SVM Score :0.0 |
| AP 1 stands as a synonym for different proteins such as c Jun , JunB , JunD , c Fos , FosB as well as the Fos related antigens Fra 1 and Fra 2 , which can either homo or heterodimerize to build up a functional transcription complex . ^^^ In nuclear extracts obtained from non tumorigenic cells , Jun family members ( in the order c Jun > JunD > JunB ) were mainly heterodimerized with Fra 1 , a protein , known to be involved in the abrogation of AP 1 activity under certain experimental conditions . ^^^ |
|
| Interacting proteins: P15407 and P17275 |
Pubmed |
SVM Score :0.0 |
| The presence of several transcription factor proteins , including JunB , JunD , c Fos , Fra 1 , CREB 1 , activating transcription factor 2 , NF KB p 50 , p 52 , and p 65 , and CAAT / enhancer binding protein delta , was demonstrated by supershift analysis . ^^^ |
|
| Interacting proteins: P15407 and P17275 |
Pubmed |
SVM Score :0.0 |
| Here , we demonstrate , by supershift analysis , that JunB , JunD , Fra 1 , Fra 2 , and c Fos bound to AP 1 but that prior treatment of the cells with TGFbeta reduced dramatically c Fos binding , suggesting that c Fos might be playing a negative regulatory role in clusterin gene expression . ^^^ |
|
| Interacting proteins: P15407 and P17275 |
Pubmed |
SVM Score :0.0 |
| In the present study , the expression of members of the AP 1 family of transcription factors in breast tumors ( n = 53 ) was investigated by Western blot with antibodies specific for each of the AP 1 family members ( c jun , junB , junD and c fos , fosB , fra 1 and fra 2 ) . ^^^ |
|
| Interacting proteins: P15407 and P17275 |
Pubmed |
SVM Score :0.0 |
| Supershift analysis with antibodies specific for NF kappaB , AP 1 , and NF IL 6 binding proteins showed that the NF kappaB binding protein included p65 / Rel A and p 50 ; AP 1 activity included c jun , junB , junD , and Fra 1 ; and NF IL 6 included C / EBPbeta . ^^^ |
|
| Interacting proteins: P15407 and P17275 |
Pubmed |
SVM Score :0.0 |
| Overexpression of Jun causes significant alterations in the composition of AP 1 , decreased junB and increased fra 1 expression and results in an increased biologic aggressiveness . ^^^ |
|
| Interacting proteins: P15407 and P17275 |
Pubmed |
SVM Score :0.0 |
| Mice lacking Fos ( encoding c Fos ) develop osteopetrosis due to an early differentiation block in the osteoclast lineage . c Fos is a component of the dimeric transcription factor activator protein 1 ( Ap 1 ) , which is composed mainly of Fos ( c Fos , FosB , Fra 1 and Fra 2 ) and Jun proteins ( c Jun , JunB and JunD ) . ^^^ |
|
| Interacting proteins: P15407 and P17275 |
Pubmed |
SVM Score :0.0 |
| In addition , AA also increased several AP 1 proteins , such as c Fos , Fra 1 , Fra 2 , JunB , JunD , and c Jun , or AP 1 and ENKCRE 2 DNA binding activity . ^^^ However , as well as proto oncoprotein levels , such as Fra 1 , Fra 2 , c Jun , JunB , but not JunD , AA induced increase of proENK mRNA was significantly reduced by the pretreatment with 10 microM of PD 98059 ( 1 . 3 fold ) or 10 microM of SB 203580 ( 1 . 8 fold ) . ^^^ In addition , the activation of both the p 38 and ERK pathways appears to be involved in the AA induced increase of proENK mRNA via activating the expression of proto oncoprotein , such as Fra 1 , Fra 2 , c Jun , and JunB . . ^^^ |
|
| Interacting proteins: P15407 and P17275 |
Pubmed |
SVM Score :0.0 |
| After cell seeding on an extracellular matrix which allows polarization and expression of the mvdp gene in response to androgens , AP 1 protein accumulation is greatly altered and consists in a loss of JunB , Fra 1 , FosB and a decrease in c Fos , c Jun and Fra 2 , while JunD remained at the same level . ^^^ |
|
| Interacting proteins: P15407 and P17275 |
Pubmed |
SVM Score :0.0 |
| Western analysis of the proteins in the nuclear extracts showed that the levels of c Jun , JunB , JunD , FosB , and Fra 2 increased and the levels of c Fos and Fra 1 decreased slightly with calcium treatment . ^^^ |
|
| Interacting proteins: P15407 and P17275 |
Pubmed |
SVM Score :0.0 |
| The AP 1 sequence specifically bound to fra 1 , junD , and junB in H 441 lung adenocarcinoma nuclear extracts . ^^^ |
|
| Interacting proteins: P15407 and P17275 |
Pubmed |
SVM Score :0.0 |
| Therefore , we studied protein expression of 6 cell cycle regulatory proteins ( Rb , p 16 , p 21 , p 27 , cyclin D 1 , and cyclin E ) in protein extracts from 53 breast cancer samples and 4 mammary cell lines and correlated the data with expression of the 7 AP 1 family members ( c jun , junB , junD , c fos , fosB , fra 1 , and fra 2 ) as determined in a previous study . ^^^ |
|
| Interacting proteins: P15407 and P17275 |
Pubmed |
SVM Score :0.0 |
| We examined by immunocytochemistry the localization of the AP 1 family proteins c Jun , JunB , JunD , c Fos , FosB , Fra 1 , and Fra 2 in rat incisor ameloblasts . ^^^ The labeling intensity of the c Jun , JunD , and Fra 2 antibodies was stronger than that of JunB , FosB , and Fra 1 . ^^^ |
|
| Interacting proteins: P15407 and P17275 |
Pubmed |
SVM Score :0.0 |
| While c Fos , Fra 2 and JunB were increased in response to either stimuli , only Fra 1 , c Jun and JunD were increased by PMA . ^^^ |
|
| Interacting proteins: P15407 and P17275 |
Pubmed |
SVM Score :0.0 |
| The constitutive expression of JunB was diminished in the undifferentiated , more aggressive tumour phenotype compared with the well differentiated , less aggressive keratinocytes , whereas the expression of other AP 1 family members ( c jun , junD , c fos , fra 1 , fra 2 and fosB ) was either very weak or variable . ^^^ Increased expression of JunB in the transfected cells resulted in up regulation of c Jun and Fra 1 and an enhanced AP 1 activity as demonstrated by transcriptional activation of the prototypic AP 1 dependent promoter , MMP 1 . ^^^ |
|
| Interacting proteins: P15407 and P17275 |
Pubmed |
SVM Score :0.0 |
| PMA significantly induced binding of JunB and Fra 1 in normal cells , while JunB and Fra 2 bound to TREs in the malignant cells . ^^^ |
|
| Interacting proteins: P15407 and P17275 |
Pubmed |
SVM Score :0.0 |
| The 138T allelic variant binds AP 1 complexes consisting primarily of c Jun , JunB and its partners Fra 1 and Fra 2 in rat VSMC . ^^^ |
|
| Interacting proteins: P15407 and P17275 |
Pubmed |
SVM Score :0.0 |
| Expression of egr 1 ( krox 24 ) , egr 2 ( krox 20 ) , egr 3 , c jun , junB , c fos , fosB , fra 1 , fra 2 , and CREB genes was analyzed by reverse transcription polymerase chain reaction ( RT PCR ) in Schwann cells isolated from neonatal rat sciatic nerves and in the cell lines MSC 80 ( mouse Schwann cells ) , NIH 3T3 ( mouse fibroblasts ) , and CHO ( Chinese hamster ovary cells ) . ^^^ |
|
| Interacting proteins: P15407 and P17275 |
Pubmed |
SVM Score :0.0 |
| Supershifts with the AP 1 oligonucleotide showed the presence of the Jun ( c Jun , JunB , JunD ) and Fos ( c Fos , FosB , Fra 1 , Fra 2 ) proteins in the untreated and TGF beta treated OA chondrocytes , whereas only Smad proteins ( Smad 2 , 3 , 4 ) were present in the AP 1 binding proteins from the TGF beta treated chondrocytes . ^^^ |
|
| Interacting proteins: P15407 and P17275 |
Pubmed |
SVM Score :0.0 |
| METHODS : We performed Western blot experiments with specific antibodies for each of the AP 1 proteins ( c jun , junB , junD , c fos , fosB , fra 1 , fra 2 ) with 41 endometrial carcinomas . ^^^ |
|
| Interacting proteins: P15407 and P17275 |
Pubmed |
SVM Score :0.0 |
| Fra 1 , Fra 2 , FosB , JunB , JunD , c Jun , and c Fos levels are increased by EGCG treatment , as is AP 1 factor binding to hINV promoter AP 1 site . ^^^ |
|
| Interacting proteins: P15407 and P17275 |
Pubmed |
SVM Score :0.0 |
| However , PMA prominently induced the binding of JunB and Fra 1 . ^^^ |
|
| Interacting proteins: P15407 and P17275 |
Pubmed |
SVM Score :0.0 |
| We have investigated the expression of the AP 1 transcription factor proteins , fos , fosB , fra 1 , fra 2 , jun , junB , junD , using Western blot analysis , in several types of asynchronously proliferating cells . ^^^ |
|
| Interacting proteins: P15407 and P17275 |
Pubmed |
SVM Score :0.0 |
| The DNA binding activities of c Fos , FosB / Delta FosB , Fra 1 , Fra 2 , and JunB were up regulated whereas JunD activity was decreased . c Fos , FosB / Delta FosB , and JunB were associated with Smad 4 . ^^^ |
|
| Interacting proteins: P15407 and P17275 |
Pubmed |
SVM Score :0.0 |
| Ribonuclease protection assays demonstrated that increases in mRNA levels of the early response protooncogenes c jun , junB , fra 1 , and fra 2 accompanied cell proliferation at low concentrations of PM whereas apoptotic concentrations of PM caused transient increases in expression of fos and jun family members and dose responsive increases in mRNA levels of receptor interacting protein , Fas associated death domain , and caspase 8 . ^^^ |
|
| Interacting proteins: P15407 and P17275 |
Pubmed |
SVM Score :0.0 |
| Treatment with exogenous TGF beta 1 resulted in a failure to induce transcription from an artificial Smad dependent promoter and a failure to down regulate c myc , but resulted in an up regulation of AP 1 associated genes ( Fra 1 , JunB and fibronectin ) . ^^^ |
|
| Interacting proteins: P15407 and P17275 |
Pubmed |
SVM Score :0.0 |
| Using DNA protein gel shift assays we determined that nerve growth factor stimulates AP 1 binding in both PC 12 and B5P cells , and identified c Fos , FosB , Fra 1 , Fra 2 , c Jun , JunB and JunD in AP 1 complexes . ^^^ |
|
| Interacting proteins: P15407 and P17275 |
Pubmed |
SVM Score :0.0 |
| The transcriptional activities of full length JunB and full length Fra 1 , but not the transactivation domain fusions , were increased by TPA treatment and suppressed by RA . ^^^ Since these full length fusions have bzip domains , the results suggest that JunB and / or Fra 1 containing dimers may constitute one target of RA for transrepression of AP 1 . . ^^^ |
|
| Interacting proteins: P15407 and P17275 |
Pubmed |
SVM Score :0.0 |
| In contrast , ERK1 / 2 , p 38 ( MAPK ) and src kinases treatment blocked nuclear translocation of almost all the AP 1 members in both models , except Fra 1 , JunD after deformation and Fra 1 , JunB after clinorotation . ^^^ |
|
| Interacting proteins: P15407 and P17275 |
Pubmed |
SVM Score :0.0 |
| Expression of c fos , fosB , fra 1 , fra 2 , and junB was low during early gestation , with a 5 to 10 fold increase on Day 23 during labor , and returned to low levels 1 day postpartum . ^^^ Administration of progesterone ( P 4 ; 16 mg / kg s . c . / day ) beginning on Day 20 ( to maintain elevated plasma P 4 levels ) prevented the onset of labor and blocked the expected rise in c fos , fosB , fra 1 , fra 2 , and junB expression on Day 23 . ^^^ In contrast , administration of the progesterone receptor antagonist RU 486 ( 10 mg / kg s . c . ) on Day 19 induced preterm labor and a premature increase in mRNA levels of c fos , fra 1 , fra 2 , and junB . ^^^ In unilaterally pregnant rats , stretch imposed by the growing fetus was found to increase the expression of c fos , fosB , fra 1 , fra 2 , and junB only in the gravid horn on the day of labor . ^^^ |
|
| Interacting proteins: P15407 and P17275 |
Pubmed |
SVM Score :0.0 |
| A functional activating protein 1 ( AP 1 ) site regulates matrix metalloproteinase 2 ( MMP 2 ) transcription by cardiac cells through interactions with JunB Fra 1 and JunB FosB heterodimers . ^^^ Antibody supershift EMSAs of nuclear extracts from normoxic rat cardiac fibroblasts demonstrated Fra 1 and JunB binding to the 1410 to 1362 bp oligonucleotide . ^^^ Nuclear extracts isolated from hypoxic rat cardiac fibroblasts contained Fra 1 , JunB and also included FosB . ^^^ Co transfection of cardiac fibroblasts with Fra 1 JunB and FosB JunB expression plasmids led to significant increases in transcriptional activity . ^^^ These studies demonstrate that a functional AP 1 site mediates MMP 2 transcription in cardiac cells through the binding of distinctive Fra 1 JunB and FosB JunB heterodimers . ^^^ |
|
| Interacting proteins: P15407 and P17275 |
Pubmed |
SVM Score :0.0 |
| Data obtained from knockout mice revealed that some components , such as c Fos are key regulators of bone cell differentiation , whereas others , like c Jun , JunB and Fra 1 are essential in embryonic and / or postnatal development . ^^^ |
|
| Interacting proteins: P15407 and P17275 |
Pubmed |
SVM Score :0.0 |
| Elevated levels of AP 1 complexes , including significant increases in c Jun , JunB and Fra 1 , were found in asbestos exposed RPM cells , but only Fra 1 expression was significantly increased and protracted in both asbestos exposed RPM cells and mesothelioma cell lines . ^^^ |
|
| Interacting proteins: P15407 and P17275 |
Pubmed |
SVM Score :0.0 |
| Gene disruption studies indicate that the AP 1 family members c jun , junB , and fra 1 are essential for embryonic development , whereas junD , c fos , and fosB are required for normal postnatal growth . ^^^ |
|
| Interacting proteins: P15407 and P17275 |
Pubmed |
SVM Score :0.0 |
| This effect was specific because palytoxin had little effect on c Jun , JunB , JunD , FosB , Fra 1 , or Fra 2 binding or on overall levels of transcription factor binding . ^^^ |
|
| Interacting proteins: P15407 and P17275 |
Pubmed |
SVM Score :0.0 |
| Phosphorylated c Jun , JunB , JunD , and Fra 1 , but not c Fos , FosB , or ATF 2 , are detected in the AP 1 DNA binding complex in cells exposed to trivalent arsenicals . ^^^ |
|
| Interacting proteins: P15407 and P17275 |
Pubmed |
SVM Score :0.0 |
| Immunoblot analyses confirmed that c Jun , JunD , JunB , c Fos , Fra 1 , Fra 2 , and FosB immunoreactive proteins were present in whole cell extracts ( WCE ) of all antral follicles and midluteal phase corpora lutea ( CL ) as well as granulosa cells ( GC ) isolated from different sized antral follicles . ^^^ |
|
| Interacting proteins: P15407 and P17275 |
Pubmed |
SVM Score :0.0 |
| In a Gal 4 fusion assay , Pdcd 4 specifically inhibited activation of c Jun and c Fos activation domains , but did not inhibit activation of JunB , JunD , Fra 1 , or Fra 2 . ^^^ |
|
| Interacting proteins: P15407 and P17275 |
Pubmed |
SVM Score :0.0 |
| Here we surveyed human lymphoma samples for expression of JunB and other AP 1 members ( c Jun , c Fos , Fra 1 , JunD ) . ^^^ |
|
| Interacting proteins: P15407 and P17275 |
Pubmed |
SVM Score :0.0 |
| The AP 1 complex typically comprises two proteins , a Jun ( c Jun , JunB , and JunD ) and a Fos ( c Fos , FosB , Fra 1 , and Fra 2 ) . ^^^ |
|
| Interacting proteins: P15407 and P17275 |
Pubmed |
SVM Score :0.0 |
| In the cerebral cortex , junB mRNA was also elevated during SD whereas , in the basal forebrain , fra 1 and fra 2 mRNA levels increased in this condition . ^^^ |
|
| Interacting proteins: P15407 and P17275 |
Pubmed |
SVM Score :0.0 |
| Transcription factors involved in the NFAT pathway including C / EBPbeta , JunB , and Fosl 1 ( Fra 1 ) , as well as Th 1 and Th 2 related transcription factors STAT 4 ( signal transducers and activators of transcription 4 ) , T bet , and c maf showed reduced expression in UCB compared with AB during primary stimulation . ^^^ |
|
| Interacting proteins: P15407 and P17275 |
Pubmed |
SVM Score :0.0 |
| RESULTS : Transcriptionally active AP 1 in combinations of c Jun , JunD and JunB with Fra 1 , Fra 2 and possibly FosB , are expressed in cardiac myocytes . ^^^ |
|
| Interacting proteins: P15407 and P17275 |
Pubmed |
SVM Score :0.0 |
| Static stretch induced an increase in the expression of c fos , fosB , fra 1 , c jun , and junB . ^^^ |
|
| Interacting proteins: P15407 and P17275 |
Pubmed |
SVM Score :0.0 |
| To determine whether NF kappaB activity is involved in the regulation of fos expression in response to various stimuli , we analyzed activity of AP 1 and expression of fos , fosB , fra 1 , fra 2 , jun , junB , and junD , as well as AP 1 downstream target gene VEGF , using MDAPanc 28 and MDAPanc 28 / IkappaBalphaM pancreatic tumor cells and wild type , IKK 1 / , and IKK 2 / murine embryonic fibroblast cells . ^^^ |
|
| Interacting proteins: P15407 and P17275 |
Pubmed |
SVM Score :0.0 |
| The upregulated genes fall into several categories including antioxidants : ceruloplasmin , metallothionein , and heme oxygenase ; antiapoptotic gene : bag 3 , chloride channels : clic 1 and clic 4 ; transcription factors : c fos , fra 1 , junB , stat 1 , krox 24 and c / ebp ; secreted signaling molecules : chitinase 3 like protein 1 and osteopontin ; inflammation related genes : MCP 1 and ICAM 1 and others . ^^^ |
|
| Interacting proteins: P15407 and P17275 |
Pubmed |
SVM Score :0.0 |
| It was demonstrated that stellate cells possess nuclear TRE binding proteins that were identified as JunB , JunD , Fra 1 , and Fra 2 , which were unaffected by either electrophiles or PMA treatment . ^^^ |
|
| Interacting proteins: P15407 and P17275 |
Pubmed |
SVM Score :0.0 |
| Moreover , supershift analysis identified that JunB and Fra 1 are major components involved in the PG mediated induction of AP 1 DNA binding . ^^^ |
|
| Interacting proteins: P15407 and P17275 |
Pubmed |
SVM Score :0.0 |
| Furthermore , experiments revealed that the protein and mRNA expression of the AP 1 subunits c Fos , Fra 1 and c Jun was reduced in lesional psoriatic skin compared with nonlesional psoriatic skin , whereas the protein and mRNA expression of the subunit JunB was increased . ^^^ Topical application of the vitamin D analogue calcipotriol under occlusion to involved psoriatic skin for 4 days resulted in an increase in AP 1 DNA binding activity , and an increase in the protein and mRNA expression of c Fos , Fra 1 and c Jun , together with a decrease in JunB protein and mRNA expression . ^^^ |
|
| Interacting proteins: P15407 and P17275 |
Pubmed |
SVM Score :0.0 |
| In our study , we investigated the AP 1 binding activity and the expression pattern of different members of the AP 1 transcription factor family ( c Jun , JunB , JunD , c Fos , FosB , Fra 1 and Fra 2 ) in different grades of cervical lesions starting from mild dysplasia to invasive cervical tumors , including normal control tissues , using specific antibodies raised against each of the AP 1 members . ^^^ |
|
| Interacting proteins: P15407 and P17275 |
Pubmed |
SVM Score :0.0 |
| However , the increased proliferation capacity of HOXB 4 overexpressing fibroblasts resulting from upregulation of JunB , Fra 1 and cyclin D 1 protein levels may indicate a potential risk associated with the HOXB 4 overexpression approach . ^^^ |
|
| Interacting proteins: P15407 and P17275 |
Pubmed |
SVM Score :0.0 |
| A gel mobility supershift analysis revealed interaction of the AP 1 factors , Fra 1 and JunB , with this element . ^^^ Treatment with 12 O tetradecanoylphorbol 13 acetate ( TPA ) , a protein kinase C activator , increased hINV gene expression , a response that correlates with increased AP 1 factor ( Fra 1 and JunB ) binding to the hINV gene AP 1 5 response element . ^^^ |
|
| Interacting proteins: P15407 and P17275 |
Pubmed |
SVM Score :0.0 |
| For this reason in this study , we investigated the expression pattern of the AP 1 family in GTDs and compared it with the expression in normal placenta using immunohistochemistry with specific polyclonal antibodies against all members of the AP 1 family ( JunB , JunD , c Jun , c Fos , FosB , Fra 1 , Fra 2 ) . ^^^ |
|
| Interacting proteins: P15407 and P17275 |
Pubmed |
SVM Score :0.0 |
| Levels of other AP 1 and NF kappaB family members were either unaffected ( i . e . , JunB ) or increased ( i . e . , Fra 1 ) . ^^^ |
|
| Interacting proteins: P15407 and P17275 |
Pubmed |
SVM Score :0.0 |
| The mRNA expressions of C / EBPbeta , C / EBPdelta , C / EBPgamma , c Jun , JunB , c Fos , Fra 1 and IkappaBalpha and protein level of IkappaBalpha were all unaffected by VIP . ^^^ |
|
| Interacting proteins: P15407 and P17275 |
Pubmed |
SVM Score :0.0 |
| Immunodepletion gel shift assays demonstrated that GP 5 EpRE bound JunB , c Jun , FosB , and Fra 2 from unstimulated cells , and that after exposure to HNE , EpRE binding complexes contained nuclear factor erythroid 2 related factor ( Nrf ) 1 , Nrf 2 , JunB , c Jun , FosB , c Fos , Fra 1 , and Fra 2 . ^^^ |
|
| Interacting proteins: P15407 and P17275 |
Pubmed |
SVM Score :0.0 |
| Converging results consolidate activator protein 1 ( AP 1 ) transcription factor as the pivotal downstream effector in the early response of stress sensitive cells to mechanical loading , and the Fra 1 , Fra 2 , JunB and JunD members of the AP 1 transcription factor family , as mediators in bone remodelling and apoptotic phenomena . ^^^ The aim of the present study was to examine the involvement of the Fra 1 , Fra 2 , JunB and JunD proteins in the biochemical response of functionally loaded mandibular condylar cartilage , and the subsequent initiation of cartilage maturation and apoptotic phenomena . ^^^ The condylar cartilage of both groups was immunostained using specific antibodies against Fra 1 , Fra 2 , JunB and JunD . ^^^ Statistical analysis of the data revealed over expression of Fra 1 , Fra 2 , JunB and JunD proteins in all stages of differentiation of chondrocytes derived from the mandibular condylar cartilage of animals fed on a hard diet . ^^^ |
|
| Interacting proteins: P15407 and P17275 |
Pubmed |
SVM Score :0.0 |
| Embryonic lethality of various AP 1 knock outs , e . g . for Jun , JunB , Fra 1 and Fra 2 largely prevented functional studies in vivo . ^^^ |
|
| Interacting proteins: P15407 and P17275 |
Pubmed |
SVM Score :0.0 |
| For this reason , nine homologues of the AP 1 leucine zipper region have been characterized : Fos ( c Fos , FosB , Fra 1 , and Fra 2 ) , Jun ( c Jun , JunB , and JunD ) , and semirational library designed winning peptides FosW and JunW . ^^^ |
|
| Interacting proteins: P15407 and P17275 |
Pubmed |
SVM Score :0.0 |
| In addition , hnRNP G overexpression led to up regulation of the expression of TXNIP , a cell cycle inhibitory gene , and significantly reduced the expression of the genes that promote cellular proliferation , such as EGR 1 , JUND , JUNB , FOS , FOSL 1 , ROS , and KIT . ^^^ |
|
| Interacting proteins: P15407 and P17275 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15407 and P17275 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15407 and P17275 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15407 and P17275 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15407 and P17275 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15407 and P17275 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15407 and P17275 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15407 and P17275 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15407 and P17275 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15407 and P17275 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15407 and P17275 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15407 and P17275 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15407 and P17275 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15407 and P17275 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15407 and P17275 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15407 and P17275 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15407 and P17275 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15407 and P17275 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15407 and P17275 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15407 and P17275 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15407 and P17275 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15407 and P17275 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15407 and P17275 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15407 and P17275 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15407 and P17275 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15407 and P17275 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15407 and P17275 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15407 and P17275 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15407 and P17275 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15407 and P17275 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15407 and P17275 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15407 and P17275 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15407 and P17275 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15407 and P17275 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15407 and P17275 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15407 and P17275 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15407 and P17275 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15407 and P17275 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15407 and P17275 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15407 and P17275 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15407 and P17275 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15407 and P17275 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15407 and P17275 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15407 and P17275 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15407 and P17275 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15407 and P17275 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15407 and P17275 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15407 and P17275 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15407 and P17275 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15407 and P17275 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15407 and P17275 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15407 and P17275 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15407 and P17275 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15407 and P17275 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15407 and P17275 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15407 and P17275 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15407 and P17275 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15407 and P17275 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15407 and P17275 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15407 and P17275 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15407 and P17275 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15407 and P17275 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15407 and P17275 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15407 and P17275 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15407 and P17275 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15407 and P17275 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15407 and P17275 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15407 and P17275 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15407 and P17275 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15407 and P17275 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15407 and P17275 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15407 and P17275 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15407 and P17275 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15407 and P17275 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15407 and P17275 |
Pubmed |
SVM Score :0.0 |
| NA |
|