| Interacting proteins: Q96A00 and P17252 |
Pubmed |
SVM Score :1.5625643 |
| Purified PKC delta was 3 fold more reactive with CPI 17 compared with myelin basic protein , whereas purified PKC alpha and recombinant RhoA activated kinases ( Rho associated coiled coil forming protein Ser / Thr kinase and protein kinase N ) showed equal activity with CPI 17 and myelin basic protein . inhibited CPI 17 phosphorylation by purified PKC delta with IC ( 50 ) of 0 . 6 microm ( in the presence of 0 . 1 mm ATP ) or 14 microm ( 2 . 0 mm ATP ) . significantly suppressed CPI 17 phosphorylation in smooth muscle cells , and the contraction of permeabilized rabbit femoral artery induced by stimulation with phorbol ester . 1.5625643^^^ |
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| Interacting proteins: Q96A00 and P17252 |
Pubmed |
SVM Score :0.0 |
| Western blot analyses showed that the content of the PKC alpha isoform ( PKCalpha ) was markedly reduced and that the smooth muscle specific protein phosphatase 1 inhibitor protein CPI 17 was not detectable , while the amount of calponin and actin still remained similar to those of intact strips . 3 . ^^^ |
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| Interacting proteins: Q96A00 and P17252 |
Pubmed |
SVM Score :0.0 |
| Dominant negative PKC alpha abolished the effect of CPI 17 on actin cytoskeleton , suggesting that the PKC alpha isoform is most likely responsible for CPI 17 activation in the endothelium . ^^^ |
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| Interacting proteins: Q96A00 and P17252 |
Pubmed |
SVM Score :0.0 |
| Furthermore , it is not certain whether regulation of MYPT by CPI 17 or by ROCKII is due to cross talk between RhoA and PKC alpha . ^^^ Acetylcholine induced 1 ) sustained phosphorylation of PKC alpha , CPI 17 , and MYPT ; 2 ) an increase in the association of phospho MYPT with HSP 27 in the particulate fraction ; 3 ) a decrease in myosin phosphatase activity ( 66 . 21+ / 3 . 52 and 42 . 19+ / 3 . 85 % nM / ml lysate at 30 s and 4 min ) ; and 4 ) an increase in PKC activity ( 298 . 12+ / 46 . 60 % and 290 . 59+ / 22 . 07 % at 30 s and 4 min ) . ^^^ Inhibition of PKCs or calphostin C or selective inhibition of PKC alpha by negative dominant constructs inhibited phosphorylation of MYPT and CPI 17 . ^^^ |
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| Interacting proteins: Q96A00 and P17252 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q96A00 and P17252 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q96A00 and P17252 |
Pubmed |
SVM Score :0.0 |
| NA |
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