Pubmed abstracts for Protein-Protein Interaction search result :

Interacting proteins: P16671 and P07996	Pubmed	SVM Score :0.97958697
A cell adhesion receptor , CD 36 , has been shown to interact with a ligand TSP 1 . 0.97958697^^^
Interacting proteins: P16671 and P07996	Pubmed	SVM Score :0.5431271
These studies indicate that GPIIIb binds to the TSP via the C terminal region and / or the CSVTCG motif , but not to the N terminal region . 0.5431271^^^
Interacting proteins: P16671 and P07996	Pubmed	SVM Score :0.50389546
We investigated CD 36 expression and the effect of TSP 1 on megakaryocytopoiesis , with and without pegylated recombinant human megakaryocyte growth and development factor ( PEG rHuMGDF ) , and with and without blocking TSP 1 binding with receptor CD 36 on megakaryocytic cells . 0.50389546^^^
Interacting proteins: P16671 and P07996	Pubmed	SVM Score :0.0
Glycoprotein IIIb ( GPIV , CD 36 ) has been proposed as the platelet receptor for thrombospondin ( TSP ) . ^^^
Interacting proteins: P16671 and P07996	Pubmed	SVM Score :0.0
Cerebral microvessels with sequestered PRBC were shown by immunohistochemistry to possess CD 36 , TSP and ICAM 1 . ^^^
Interacting proteins: P16671 and P07996	Pubmed	SVM Score :0.0
The anti sense PECAM 1 transfected cells turn on expression of endogenous TSP 1 and its angioinhibitory receptor , CD 36 . ^^^
Interacting proteins: P16671 and P07996	Pubmed	SVM Score :0.0
The antiangiogenic activity of TSP 1 is mediated by the binding of properdin like type 1 repeats to the receptor CD 36 . ^^^
Interacting proteins: P16671 and P07996	Pubmed	SVM Score :0.0
CD 36 expressed on the surface of endothelial cells functions as the primary antiangiogenic receptor for TSP 1 . ^^^
Interacting proteins: P16671 and P07996	Pubmed	SVM Score :0.0
All three cell lines expressed the TSP 1 receptor CD 36 . ^^^
Interacting proteins: P16671 and P07996	Pubmed	SVM Score :0.0
TSP colocalized with CD 36 on granulosa cells ( GC ) in the follicle and in cultured cells . ^^^
Interacting proteins: P16671 and P07996	Pubmed	SVM Score :0.0
Under these conditions , immobilized GP 4 was found to specifically bind TSP , whereas it was unreactive with fibrinogen . ^^^
Interacting proteins: P16671 and P07996	Pubmed	SVM Score :0.0
The downregulation of CD 36 mRNA was associated with the disappearance of surface expression of CD 36 antigen and loss of TSP 1 surface binding capacity . ^^^
Interacting proteins: P16671 and P07996	Pubmed	SVM Score :0.0
For the confirmation of the polyether ionophore antibiotic , maduramicin alpha , in chicken fat , the techniques of desorption chemical ionization ( DCI ) , thermospray liquid chromatography / mass spectrometry ( TSP LC / MS ) , and thermospray liquid chromatography / tandem mass spectrometry ( TSP LC / MS / MS ) were investigated as potential approaches . ^^^
Interacting proteins: P16671 and P07996	Pubmed	SVM Score :0.0
The original confirmatory method for moxidectin in cattle fat , the target tissue for regulatory purposes , was LC with mass spectrometry ( MS ) using thermospray ( TSP ) ionization and selected ion monitoring . ^^^ As newer ionization techniques for LC / MS made TSP obsolete and with the availability of a new generation of benchtop LC / MS instrumentation , the confirmation of moxidectin in cattle fat was re evaluated . ^^^
Interacting proteins: P16671 and P07996	Pubmed	SVM Score :0.0
To determine the specific receptor systems that mediate the interaction of tumor cells with insoluble TSP , the attachment of HT 1080 fibrosarcoma and C 32 and G 361 melanoma cells to TSP coated discs was studied in the presence of heparin , Arg Gly Asp Ser , or antibodies to glycoprotein ( GP ) 4 ( CD 36 , GPIIIb ) , a TSP receptor . ^^^
Interacting proteins: P16671 and P07996	Pubmed	SVM Score :0.0
GPIIIb bound to surface immobilized thrombospondin ( TSP ) in a concentration dependent and saturable manner , with approximately 60 % reduction in binding in the presence of EDTA . ^^^ PAS 4 bound to TSP with similar characteristics except that maximum binding was consistently approximately 50 % of that of GPIIIb and binding was not inhibited by EDTA . ^^^
Interacting proteins: P16671 and P07996	Pubmed	SVM Score :0.0
Glycoprotein 4 ( GPIV ; CD 36 or GPIIIb ) is a cell surface glycoprotein that has been proposed as mediating a number of physiologically important processes such as the adhesion of platelets to thrombospondin ( TSP ) and collagen , the cytoadherence of Plasmodium falciparum infected erythrocytes , and the TSP dependent interaction of monocytes with platelets and macrophages . ^^^
Interacting proteins: P16671 and P07996	Pubmed	SVM Score :0.0
Double labeling experiments showed colocalization of Fg and TSP , GPIV and TSP , as well as Fg and GPIIb IIIa , although no typical coclustering of GPIIb IIIa and GPIV or GPIIb IIIa and p 24 was apparent . ^^^
Interacting proteins: P16671 and P07996	Pubmed	SVM Score :0.0
The even greater adherence ( P < . 005 ) of sickle RBCs in autologous platelet rich plasma ( without added TSP ) is dramatically inhibited by alpha CD 36 antibodies ( OKM 5 and alpha GPIV ) and significantly diminished by alpha TSP , by peptides RGDS and CSVTCG , and by two antibodies to the vitronectin receptor ( 7E3 and LM 609 ) . ^^^
Interacting proteins: P16671 and P07996	Pubmed	SVM Score :0.0
Using specific antibodies to two intrinsic plasma membrane glycoproteins ( GPs ) , the GPIIb IIIa complex and GPIV , and to thrombospondin ( TSP ) , a a granular protein that becomes expressed on the platelet surface upon activation , we were able to distinguish two groups of patients : 16 patients presented an increased concentration of TSP on their platelets demonstrating in vivo platelet secretion . ^^^
Interacting proteins: P16671 and P07996	Pubmed	SVM Score :0.0
TSP dependent adhesion was inhibited by anti TSP fab fragments , an anti TSP monoclonal antibody , an RGD containing peptide , complex specific anti glycoprotein ( GP ) IIb IIIa monoclonal antibodies ( A2A9 or AP 2 ) and anti VLA 2 monoclonal antibodies ( 6F1 and Gi 9 ) , but not by rabbit preimmune fab fragments , mouse IgG , an anti GPIIIa monoclonal antibody , or monoclonal antibodies against either the human vitronectin receptor , glycocalicin , or GPIV . ^^^
Interacting proteins: P16671 and P07996	Pubmed	SVM Score :0.0
We now show that TSP dependent platelet monocyte interactions are mediated by glycoprotein 4 ( GPIV ) , an intrinsic membrane protein recently identified as a cell surface TSP receptor . ^^^ Monoclonal antibodies to GPIV bound to cells of the human monocytoid line U 937 as assessed by flow cytometry and inhibited the binding of 125I TSP to the cell surface by 83 % . ^^^ These data suggest that TSP can cross link platelets and monocytes via an interaction with GPIV on the surface of both cells . ^^^
Interacting proteins: P16671 and P07996	Pubmed	SVM Score :0.0
The TSP promoted aggregation of nonstimulated platelets was inhibited by a monoclonal antibody to platelet glycoprotein 4 ( GPIV ) , but not by a monoclonal antibody to the fibrinogen receptor , GPIIb IIIa . ^^^ In contrast , the antibody to GPIIb IIIa totally inhibited the TSP potentiated aggregation of thrombin stimulated platelets , whereas the antibody to GPIV has no effect . ^^^
Interacting proteins: P16671 and P07996	Pubmed	SVM Score :0.0
But , in contrast to chemotaxis , neither pertussis toxin nor cholera toxin inhibited TSP mediated haptotaxis , suggesting the involvement of a different signal transduction pathway . mAbs against GPIV , beta 1 , beta 3 , or alpha 5 integrins did not affect monocyte chemotaxis or haptotaxis , ruling out the involvement of these receptors . ^^^
Interacting proteins: P16671 and P07996	Pubmed	SVM Score :0.0
The adhesion was not inhibited with known antagonists of the GPIV TSP interaction , nor by inhibitors of several other known binding domains in TSP . ^^^ These results suggest that a mechanism distinct from GPIV is responsible for sickle RBC adhesion to immobilized TSP under flow conditions . . ^^^
Interacting proteins: P16671 and P07996	Pubmed	SVM Score :0.0
The redistribution of platelet membrane glycoprotein 4 ( GPIV ) and the release of intracellular alpha granule thrombospondin ( TSP ) were examined and the inhibition of beta thromboglobulin ( beta TG ) and platelet factor 4 ( PF 4 ) in patients with chronic myelogenous leukemia ( CML ) was observed and quantitation of beta TG and PF 4 in sera was conducted . ^^^ There was no direct correlation between GPIV expression and TSP binding after platelet activation . ^^^
Interacting proteins: P16671 and P07996	Pubmed	SVM Score :0.0
AIM : To study the possibility of dauricine ( Dau ) inhibiting redistribution of platelet membrane glycoprotein 4 ( GPIV ) and release of intracellular alpha granule thrombospondin ( TSP ) on platelet activation . ^^^ METHODS : Using the flow cytometric assay of washed platelet to record expression of GPIV and release of TSP induced by thrombin . ^^^ RESULTS : Dau did not affect GPIV and TSP on resting platelet membrane but inhibited redistribution of GPIV to the platelet surface and TSP release on activated platelet . ^^^ There was a marked positive correlation between changes of GPIV and TSP ( r = 0 . 511 , P < 0 . 01 ) . ^^^
Interacting proteins: P16671 and P07996	Pubmed	SVM Score :0.0
Patients having experienced a thrombotic event had a higher mean percentage of TSP positive non stimulated platelets than patients without a history of thrombosis ( 9 . 0 % vs . 4 . 6 % ; P = 0 . 02 ) and a higher GPIV molecules of equivalent fluorochrome ( MEF ) value ( 33113 vs . 24471 MEF ; P = 0 . 02 ) . ^^^
Interacting proteins: P16671 and P07996	Pubmed	SVM Score :0.0
In addition , this MoAb inhibited in a dose dependent manner ( IC 50 approximately 0 . 5 mumol / L ) the interaction of 125I TSP with immobilized fibrinogen and platelet glycoprotein 4 , both potential physiologic receptors for TSP on thrombin activated platelets . ^^^
Interacting proteins: P16671 and P07996	Pubmed	SVM Score :0.0
Using a human skin / severe combined immunodeficient ( SCID ) chimeric mouse model , we examined the keratinocyte expression of the thrombospondin receptor ( CD 36 ) and its ligand thrombospondin 1 ( TSP 1 ) in acute uninflamed wounds . ^^^
Interacting proteins: P16671 and P07996	Pubmed	SVM Score :0.0
A number of host cell molecules such as CD 36 , thrombospondin ( TSP ) and intercellular adhesion molecule 1 ( ICAM 1 ) may function as endothelial cell surface receptors for P . falciparum infected erythrocytes . ^^^ Affinity labeling of CD 36 and TSP to the PRBC surface showed these molecules specifically bind to the knobs . ^^^
Interacting proteins: P16671 and P07996	Pubmed	SVM Score :0.0
To clarify the role of CD 36 as a TSP receptor and to investigate the mechanisms of the TSP CD 36 interaction , transfection studies were performed using CD 36 cDNA in a CDM 8 plasmid . ^^^ The TSP amino acid sequence , CSVTCG , mediates the interaction of thrombospondin with CD 36 . ^^^
Interacting proteins: P16671 and P07996	Pubmed	SVM Score :0.0
In vitro studies indicate that sequestration of PRBC in the microvessels is mediated by the attachment of knobs on PRBC to receptors on the endothelial cell surface such as CD 36 , thrombospondin ( TSP ) , and intercellular adhesion molecule 1 ( ICAM 1 ) . ^^^ Cerebral microvessels with sequestered PRBC were shown by immunohistochemical analysis to possess CD 36 , TSP , and ICAM 1 . ^^^
Interacting proteins: P16671 and P07996	Pubmed	SVM Score :0.0
To clarify and confirm the function of CD 36 ( glycoprotein 4 ) as a TSP receptor , we now describe a transfected cell model using human melanoma cells genetically manipulated by sense or antisense cDNA transfection to express either high or near zero levels of CD 36 . ^^^ Bowes melanoma cells , which in their wild type did not express CD 36 and did not bind radiolabeled TSP , when transfected with the sense construct bound TSP in a 1 : 1 stoichiometric ratio with CD 36 expression . ^^^ Conversely , C 32 melanoma cells , which in their wild type expressed high levels of CD 36 and bound radiolabeled TSP at a 1 : 1 stoichiometric ratio , did not express CD 36 and did not bind TSP when transfected with an antisense construct . ^^^ These data , i . e . the gain of function with sense cDNA transfection and loss of function with antisense transfection , strongly support the TSP receptor function of CD 36 . ^^^ The distribution of this protein in vascular cells and tissues and observations that it may participate in signal transduction events suggest that TSP CD 36 interactions may play a role in mediating some of the pathophysiological processes associated with TSP . . ^^^
Interacting proteins: P16671 and P07996	Pubmed	SVM Score :0.0
Both CD 36 and thrombospondin ( TSP ) are glycoproteins that mediate PRBC adherence to endothelial cells in vitro . ^^^ By applying affinity labeling of receptor binding sites with purified ligands , we showed for the first time that both CD 36 and TSP can bind independently to the PRBC surface and that the PRBC receptor ( s ) for CD 36 and TSP are localized specifically to the electron dense knob protrusions of the PRBC surface . ^^^
Interacting proteins: P16671 and P07996	Pubmed	SVM Score :0.0
CD 36 ( glycoprotein 4 or IIIB ) is an integral plasma membrane protein of wide cellular distribution and functions as a receptor site for thrombospondin ( TSP ) , an adhesive protein important in cell cell and cell matrix interactions . ^^^ Surprisingly , P 139 155 significantly augmented , instead of inhibited , binding of CD 36 to TSP . ^^^ Another CD 36 peptide , P 93 110 , which had no effect on OKM 5 immunoadsorption , blocked binding of CD 36 to immobilized TSP and partially inhibited collagen induced platelet aggregation . ^^^ The data suggest that CD 36 TSP interaction is a two step process ; the sequence 139 155 region of CD 36 binds first to TSP , triggering a change in TSP to reveal a second site , which binds the 93 110 region of CD 36 with high affinity . ^^^ CD 36 peptides can be used as stimulators or inhibitors in cellular adhesive events involving TSP CD 36 interaction . ^^^
Interacting proteins: P16671 and P07996	Pubmed	SVM Score :0.0
Furthermore , inhibition with mAbs / Arg Gly Asp Ser peptide of the candidate M phi receptors for TSP , CD 36 , and alpha 5 beta 3 exerted synergistic effects on both M phi recognition of aged PMN and M phi adhesion to TSP , indicating that `` two point ' ' adhesion of TSP to these M phi structures is involved in phagocytosis of aged PMN . ^^^ Our findings indicate newly defined roles for TSP and CD 36 in phagocytic clearance of senescent neutrophils , which may limit inflammatory tissue injury and promote resolution . . ^^^
Interacting proteins: P16671 and P07996	Pubmed	SVM Score :0.0
On the basis of our experiments , we conclude that , CD 36 molecule , a receptor for TSP , is synthesized and expressed in at least two ways , inside the cells and on the surface of megakaryocyte lineage leukemias and megakaryocytic leukemia cell lines MEG 01 and HEL . ^^^ This is strongly suggestive that thrombospondin ( TSP ) mediated adhesion represents an alternative pathway for cytoadherence , and that CD 36 expression on various kinds of cells may lack some essential modifications or components necessary for the TSP receptor activity . . ^^^
Interacting proteins: P16671 and P07996	Pubmed	SVM Score :0.0
Crosscompetition tests confirmed that the interaction site ( s ) of TSP with monocytes are distinct from the signal promoting sites recognized by polyclonal and 3 monoclonal anti CD 36 antibodies . . ^^^
Interacting proteins: P16671 and P07996	Pubmed	SVM Score :0.0
The protein CD 36 is a membrane receptor for thrombospondin ( TSP ) , malaria infected erythrocytes , and collagen . ^^^ Three functional sequences were identified within a single disulfide loop of CD 36 : one that mediates TSP binding ( amino acids 87 to 99 ) and two that support malarial cytoadhesion ( amino acids 8 to 21 and 97 to 110 ) . ^^^ Dephosphorylation of constitutively phosphorylated CD 36 in resting platelets and a megakaryocytic cell line led to the loss of collagen adhesion and platelet reactivity to collagen , with a reciprocal increase in TSP binding . ^^^ In site directed mutagenesis studies , when the threonine phosphorylation site was changed to alanine , CD 36 was expressed in a dephosphorylated state and bound to TSP constitutively . . ^^^
Interacting proteins: P16671 and P07996	Pubmed	SVM Score :0.0
The adhesion molecules examined were the leukocyte function antigen ( LFA 1 ) and its ligand intercellular adhesion molecule 1 ( ICAM 1 ) , the receptor alpha 5 beta 3 for vitronectin , its subunits alpha 5 and beta 3 , and the CD 36 receptor for thrombospondin ( TSP ) . ^^^ In contrast , expression of TSP and the CD 36 receptor for TSP were not related to metastatic potential . ^^^ They do not support a role for CD 44 , Leu 8 , CLA and TSP or its receptor CD 36 in the metastatic process in melanoma . . ^^^
Interacting proteins: P16671 and P07996	Pubmed	SVM Score :0.0
The state of inflammation of these endothelial cells was investigated through the use of a panel of monoclonal antibodies ( MAb ) directed against thrombospondin ( TSP ) , von Willebrand factor ( vWF ) , integrins ( alpha IIb beta 3 , alpha 5 beta 3 ) , CD 36 , and classical markers of inflammation ( P selectin , E selectin , ICAM 1 , VCAM ) . ^^^
Interacting proteins: P16671 and P07996	Pubmed	SVM Score :0.0
Comparison of TSP immunolocalization with that of two known TSP cell surface receptors , CD 36 and CD 51 , reveals no codistribution of TSP with these receptors during mammary gland development . ^^^ As opposed to TSP , CD 36 is strongly expressed at the membrane of preadipocytes present in the fat pad tissue , but absent from budding epithelial cells . ^^^ In lactating ducts of a newborn , TSP disappears from the myoepithelial stromal junction of ducts and is synthesized at the apices of secretory epithelial cells of lactating ducts together with CD 36 . ^^^
Interacting proteins: P16671 and P07996	Pubmed	SVM Score :0.0
We have characterized the adhesion of flowing parasitized red blood cells to three of the known endothelial receptors coated on plastic surfaces ( CD 36 , intercellular adhesion molecule 1 ( ICAM 1 ) and thrombospondin ( TSP ) ) , and also to cells bearing these receptors ( human umbilical vein endothelial cells ( HUVEC ) and platelets ) . ^^^ The great majority of adherent parasitized cells formed rolling rather than static attachments to HUVEC and ICAM 1 , whereas static attachments predominated for platelets , CD 36 and TSP . ^^^ Adhesion via ICAM 1 was least sensitive to increasing wall shear stress , but absolute efficiency of adhesion was greatest for CD 36 , followed by ICAM 1 , and least for TSP . ^^^ TSP did not give long lasting adhesion under flow , whereas cells remained adherent to CD 36 or ICAM 1 . ^^^ Initial interaction at high wall shear stress may be of a rolling type , mediated by ICAM 1 or other receptors , with immobilization and stabilization occurring via CD 36 and / or TSP . . ^^^
Interacting proteins: P16671 and P07996	Pubmed	SVM Score :0.0
Expression of thrombospondin 1 ( TSP 1 ) and its receptor ( CD 36 ) in healthy and diseased human skin . ^^^ In the present study , an analysis was made of the expression pattern of thrombospondin 1 ( TSP 1 ) and its receptor ( CD 36 ) in skin biopsies obtained from healthy volunteers and from patients with lichen planus , lupus erythematosus , cutaneous T cell lymphoma and psoriasis vulgaris . ^^^ In all cases investigated , the CD 36 positive lesional keratinocytes remained TSP 1 negative . ^^^
Interacting proteins: P16671 and P07996	Pubmed	SVM Score :0.0
CD 36 is a multifunctional cell surface glycoprotein that acts as a surface receptor for thrombospondin ( TSP ) , and thereby may mediate adhesive interactions between cells and substrata , platelets and other cells , and macrophages and apoptotic neutrophils . ^^^ The identity of the TSP binding site on CD 36 is controversial and may involve more than one structural domain . ^^^ We have constructed a series of recombinant bacterial GST / CD36 fusion proteins that span nearly all of the CD 36 molecule and have demonstrated that fusion proteins containing the region extending from amino acid 93 to 120 formed specific , saturable , and reversible complexes with TSP . ^^^ As with intact CD 36 , binding was calcium dependent , was independent of which ligand was immobilized , and was blocked by monoclonal antibodies to both CD 36 and TSP . ^^^ We also demonstrated that these fusion proteins competitively inhibited binding of TSP to purified platelet CD 36 and to cell surface CD 36 on peripheral blood monocytes and CD 36 cDNA transfected melanoma cells . ^^^
Interacting proteins: P16671 and P07996	Pubmed	SVM Score :0.0
In each case , the cytokine expanded phagocytic subpopulation employed the thrombospondin ( TSP ) dependent recognition mechanism defined for mature M phi , in which M phi vitronectin receptor and CD 36 cooperate . ^^^ However , commensurate increases in M phi expression of VnR , TSP , or CD 36 were not detectable , indicating that TSP mediated recognition can be recruited by other mechanisms . ^^^
Interacting proteins: P16671 and P07996	Pubmed	SVM Score :0.0
TSP is secreted ( 80 + / 6 ng TSP 10 ( 6 ) cells ) and bound to the surface of M3Da . cells via receptors different from CD 36 , as shown by biosynthetic labelling and immunofluorescence studies . ^^^
Interacting proteins: P16671 and P07996	Pubmed	SVM Score :0.0
Expression of thrombospondin ( TSP 1 ) and its receptors ( CD 36 and CD 51 ) in normal , hyperplastic , and neoplastic human breast . ^^^ Its immunolocalization was also compared with that of two known cell surface receptors for TSP 1 : CD 36 and CD 51 . ^^^ However , TSP 1 becomes selectively expressed at the apices of secretory epithelial cells of lactating ducts together with CD 36 , suggesting that the distribution of TSP 1 and the appearance of its receptors are dependent on the secretory activity of human mammary ducts . ^^^ On the other hand , few invasive ductal carcinoma cells ( 10 % ) express TSP 1 , while CD 51 is moderately expressed by some neoplastic clusters , and no immunoreactivity is observed for CD 36 . ^^^ By contrast , TSP 1 is codistributed with CD 51 in most of the invasive lobular carcinoma cells ( 40 to 80 % ) and with CD 36 in a subpopulation ( 30 to 40 % ) of these invasive tumor cells . ^^^
Interacting proteins: P16671 and P07996	Pubmed	SVM Score :0.0
Thrombospondin ( TSP ) binds to its cellular receptor , CD 36 ( glycoprotein 4 or IIIb ) , and participates in many adhesive cell interactions . ^^^ We have shown that the CD 36 TSP interaction occurs in a stepwise , conformation dependent process . ^^^ CD 36 sequence 139 155 binds TSP and induces a second CD 36 binding site , which binds CD 36 sequence 93 110 with high affinity . ^^^ To characterize this high affinity CD 36 binding site on TSP , an anti TSP monoclonal antibody ( mAb ) , 7A 1e , was identified that showed augmentation of TSP binding in the presence of CD 36 peptide P 139 155 . ^^^ Purified mAb 7A 1e IgG specifically blocked both P 139 155 augmented CD 36 binding and P 139 155 dependent CD 36 peptide P 93 110 binding to TSP , indicating that the binding sites on TSP for mAb 7A 1e and P 93 110 are closely related . mAb 7A 1e IgG inhibited collagen induced platelet aggregation in platelet rich plasma . ^^^
Interacting proteins: P16671 and P07996	Pubmed	SVM Score :0.0
It may serve as a receptor for collagen and is also able to bind thrombospondin ( TSP ) , because a monoclonal antibody to CD 36 inhibits TSP binding to thrombin stimulated platelets . ^^^ In conclusion , this study shows the existence of a significant intragranular pool of CD 36 in platelets that may play a critical role in the surface expression of alpha granule TSP during platelet activation . . ^^^
Interacting proteins: P16671 and P07996	Pubmed	SVM Score :0.0
When MEC were incubated with an antibody ( OKM 5 ) against CD 36 ( a thrombospondin [ TSP ] receptor ) , platelet supernatant mediated sickle erythrocyte adherence was inhibited 86 % , suggesting that TSP participated in the adherence . ^^^
Interacting proteins: P16671 and P07996	Pubmed	SVM Score :0.0
These data demonstrate that HDMEC bind to immobilized TSP 1 in an RGD and CSVTCG independent manner via an acid labile epitope ( s ) which recognized via a receptor or receptors distinct from CD 36 or alphavbeta 3 integrin receptor . . ^^^
Interacting proteins: P16671 and P07996	Pubmed	SVM Score :0.0
We affinity purified molecules from detergent extracts of surface radioiodinated PRBCs using several endothelial cell receptors known to support PRBC adherence , including CD 36 , thrombospondin ( TSP ) , and intercellular adhesion molecule 1 ( ICAM 1 ) . ^^^ Preadsorption of PRBC extracts with anti PfEMP 1 antibodies , CD 36 , or TSP markedly reduced PfEMP 1 binding to CD 36 or TSP . ^^^ Mild trypsinization of intact PRBCs of P . falciparum strains shown to express antigenically different PfEMP 1 released different ( 125 ) 1 labeled tryptic fragments of PfEMP 1 that bound specifically to CD 36 and TSP . ^^^ In clone C 5 and strain MC , these activities resided on different tryptic fragments , but a single tryptic fragment from clone ItG ICAM bound to both CD 36 and TSP . ^^^ Hence , the CD 36 and TSP binding domains are distinct entities located on a single PfEMP 1 molecule . ^^^
Interacting proteins: P16671 and P07996	Pubmed	SVM Score :0.0
In this study , we investigate adherence to C 32 melanoma cells , CD 36 , intracellular adhesion molecule 1 ( ICAM 1 ) , thrombospondin ( TSP ) , E selectin , vascular cell adhesion molecule 1 ( VCAM 1 ) , and chondroitin sulfate A ( CSA ) of 36 P . falciparum isolates from patients suffering from acute falciparum malaria . ^^^
Interacting proteins: P16671 and P07996	Pubmed	SVM Score :0.0
Examination of infected tissues indicate PRBC sequestration in microvessels is the result of PRBC knob attachment to endothelial cell surface cytoadherence receptors such as CD 36 , thrombospondin ( TSP ) , and intercellular adhesion molecule 1 ( ICAM 1 ) . ^^^ Immunohistochemical analysis showed CD 36 , TSP , and ICAM 1 cytoadherence proteins in several major organs . . ^^^
Interacting proteins: P16671 and P07996	Pubmed	SVM Score :0.0
However , the specific inhibitory effects of cationic aminosugars , Arg Gly Asp Ser ( RGDS ) peptide , and mAbs to phagocyte alpha ( 5 ) beta 3 vitronectin receptor integrin and `` bridging ' ' thrombospondin 1 ( TSP 1 ) indicated that mesangial cell phagocytosis of apoptotic cells involved an alpha ( 5 ) beta3 / TSP mechanism akin to that described for human monocyte derived macrophages ( Mphi ) in which Mphi CD 36 plays an important role in binding `` bridging ' ' TSP 1 . ^^^ However , mesangial cells did not express CD 36 and there was no evidence for involvement of alternative phagocyte receptors for TSP 1 , heparan sulfate proteoglycan and sulfatides . ^^^
Interacting proteins: P16671 and P07996	Pubmed	SVM Score :0.0
Here we use both native TSP 1 and small antiangiogenic peptides derived from it to show that this inhibition is mediated by CD 36 , a transmembrane glycoprotein found on microvascular endothelial cells . ^^^ Both IgG antibodies against CD 36 and glutathione S transferase CD 36 fusion proteins that contain the TSP 1 binding site blocked the ability of intact TSP 1 and its active peptides to inhibit the migration of cultured microvascular endothelial cells . ^^^ In addition , antiangiogenic TSP 1 peptides inhibited the binding of native TSP 1 to solid phase CD 36 and its fusion proteins , as well as to CD 36 expressing cells . ^^^ Additional molecules known to bind CD 36 , including the IgM anti CD 36 antibody SM , oxidized ( but not unoxidized ) low density lipoprotein , and human collagen 1 , mimicked TSP 1 by inhibiting the migration of human microvascular endothelial cells . ^^^ Transfection of CD 36 deficient human umbilical vein endothelial cells with a CD 36 expression plasmid caused them to become sensitive to TSP 1 inhibition of their migration and tube formation . ^^^
Interacting proteins: P16671 and P07996	Pubmed	SVM Score :0.0
Sequence analysis revealed a TSP 1 recognition motif , previously defined for the CD 36 gene family of cell adhesion receptors , in conserved regions flanking the disulfide linked cysteine residues of the V 3 loop of HIV envelope glycoprotein gp 120 , important for HIV binding to its high affinity cellular receptor CD 4 . ^^^ Based on peptide blocking experiments , the TSP 1 gp120 interaction involves CSVTCG sequences in the type 1 properdin like repeats of TSP 1 , the known binding site for CD 36 . ^^^ TSP 1 and fusion proteins derived from CD 36 related TSP 1 binding domains were able to compete with radiolabeled soluble CD 4 binding to immobilized gp 120 . ^^^
Interacting proteins: P16671 and P07996	Pubmed	SVM Score :0.0
We present evidence showing that signals from CD 36 , the thrombospondin ( TSP ) receptor , can inhibit this thrombin mediated calcium response . ^^^ The presence of TSP inhibited the thrombin stimulated calcium response in CD 36 positive microvascular endothelial cells but not in CD 36 negative umbilical vein endothelial cells . ^^^ This TSP effect was mimicked by anti CD 36 antibodies and a TSP peptide ( CSVTCG ) , but not by an alternative CD 36 ligand ( collagen 4 ) or an antibody to an alternative TSP receptor ( alphavbeta 3 ) . ^^^ In addition , TSP and anti CD 36 antibodies inhibited the calcium response of a closely related receptor , the trypsin / SLIGKVD activated receptor PAR 2 . ^^^
Interacting proteins: P16671 and P07996	Pubmed	SVM Score :0.0
We recently defined a single structural domain on CD 36 that mediates binding to adhesive glycoprotein thrombospondin 1 ( TSP 1 ) . ^^^ To test whether LIMPII also binds TSP 1 , a LIMPII peptide corresponding to the TSP 1 binding domain of CD 36 was expressed as a recombinant glutathione S transferase ( GST ) fusion protein . ^^^ TSP1 complex formation was specifically blocked by soluble LIMPII fusion protein , by monospecific rabbit IgG directed against the LIMPII peptide and by CD 36 fusion proteins containing the TSP 1 binding domain . ^^^ This study defines a TSP 1 binding site conserved between LIMPII and CD 36 and suggests that cell surface LIMPII may function in some circumstances as an adhesion receptor for TSP 1 . ^^^ Computer assisted homology searches suggest that the TSP 1 recognition motif identified from study of CD 36 family members may be widely expressed in nature . . ^^^
Interacting proteins: P16671 and P07996	Pubmed	SVM Score :0.0
TSP 1 , peptides , and a recombinant fragment from the type 1 repeats , but not peptides that bind CD 36 or CD 47 , inhibit the proliferation of A 2058 melanoma cells . ^^^
Interacting proteins: P16671 and P07996	Pubmed	SVM Score :0.0
In this study , we examined the binding of soluble TSP 1 ( and ox LDL ) to CD 36 transfected cells and the mechanisms by which immobilized TSP 1 mediated attachment and haptotaxis ( cell migration towards a substratum bound ligand ) of these transfected cells . ^^^ CD 36 cDNA transfection of NIH 3T3 cells clearly induced a dramatic increase in binding of both soluble [ 125I ] TSP 1 and [ 125I ] ox LDL to the surface of CD 36 transfected cells , indicating that there was a gain of function with CD 36 transfection in NIH 3T3 cells . ^^^ Despite this gain of function , mock and CD 36 transfected NIH 3T3 cells attached and migrated to a similar extent on immobilized TSP 1 . ^^^ An anti TSP 1 oligoclonal antibody inhibited CD 36 transfected cell attachment to TSP 1 while function blocking anti CD 36 antibodies , alone or in combination with heparin , did not . ^^^ Although CD 36 binds soluble TSP 1 through a CSVTCG sequence located within type 1 repeats , 18 , 19CD36 transfected NIH 3T3 cells did not attach to immobilized type 1 repeats while they did adhere to the N terminal , type 3 repeats ( in an RGD dependent manner ) and the C terminal domain of TSP 1 . ^^^
Interacting proteins: P16671 and P07996	Pubmed	SVM Score :0.0
CD 36 is a cell adhesion receptor shown to interact with the ligand thrombospondin 1 ( TSP 1 ) . ^^^ It has not been determined whether the interaction of TSP 1 and its receptor CD 36 is correlated with vascularisation or clinical outcome of malignant tumours in vivo . ^^^ We studied the correlations between expression of TSP 1 , CD 36 and vascularisation and prognosis in 65 colon cancers . ^^^ TSP 1 expression levels did not affect CD 36 expression . ^^^
Interacting proteins: P16671 and P07996	Pubmed	SVM Score :0.0
Like whole TSP 1 , the Mal 2 D Ile derivative inhibited a broad range of angiogenic inducers , was selective for endothelial cells , and required CD 36 receptor binding for activity . ^^^
Interacting proteins: P16671 and P07996	Pubmed	SVM Score :0.0
The attachment of both SK N SH and U 251MG cells to TSP 1 was found to be mediated by heparan sulfate proteoglycans , integrins , and the CLESH 1 adhesion domain first identified in CD 36 . ^^^ Treatment with a pharmacologic dosage of retinoic acid altered the TSP 1 cell adhesion mechanism in both cell lines in that neither heparin nor micromolar concentrations of the RGD peptide inhibited attachment ; after treatment , attachment was inhibited by the CSVTCG peptide located in the type 1 repeat domain of TSP 1 and a recombinant adhesion domain ( CLESH 1 ) from CD 36 . ^^^
Interacting proteins: P16671 and P07996	Pubmed	SVM Score :0.0
A peptide of LF , Arg Asn Met Arg Lys Val Arg Gly Pro Pro Val Ser Cys ( amino acid residues 25 37 of LF ) , which has been suggested to contribute to LF binding to various materials , including CSA , inhibited PE binding to immobilized CSA conjugated albumin , immobilized CD 36 , CD 36 expressing CHO cells , immobilized TSP , and C 32 amelanotic melanoma cells , as well as LF itself . ^^^ These results suggest that LF peptide may provide the basis for developing agents that are able to inhibit CSA , CD 36 , and TSP mediated cytoadherence of PE . . ^^^
Interacting proteins: P16671 and P07996	Pubmed	SVM Score :0.0
However , TSP 1 overexpression up regulated the TSP 1 receptor CD 36 , leading to enhanced adhesion of A 431 cells to TSP 1 . ^^^
Interacting proteins: P16671 and P07996	Pubmed	SVM Score :0.0
Using immunofluorescence and antibodies to TGF beta 1 and CD 36 , a receptor for TSP 1 , there was colocalization of TGF beta 1 with CD 36 . ^^^ Because TSP 1 but not TGF beta 1 is a natural ligand for CD 36 , these findings suggest that the L TGF beta 1 in a complex with TSP 1 localizes to the macrophage cell surface when TSP 1 interacts with its receptor , CD 36 . ^^^ Furthermore , the association of TSP 1 / L TGF beta 1 complex with CD 36 is necessary to the activation of L TGF beta 1 because antibodies to CD 36 prevent the colocalization of TGF beta 1 with CD 36 as observed by immunofluorescence and inhibit activation of the L TGF beta 1 by explanted alveolar macrophages . ^^^
Interacting proteins: P16671 and P07996	Pubmed	SVM Score :0.0
However , TSP and its receptor CD 36 are abundantly expressed in chronically inflamed tissues such as the rheumatoid synovium . ^^^ Data presented reveal that TSP mediated costimulation is achieved through its independent interaction with CD 36 on APCs and with CD 47 on T cells . ^^^ We propose that a CD 47 TSP CD 36 trimolecular complex is a novel costimulatory pathway that significantly decreases the threshold of T cell activation . ^^^
Interacting proteins: P16671 and P07996	Pubmed	SVM Score :0.0
AIM : To describe the pattern of distribution of thrombospondin ( TSP 1 ) and its receptors , alpha root of beta 3 integrin and CD 36 , in normal human thyroid tissue and to compare their expression in different benign and malignant thyroid conditions . ^^^
Interacting proteins: P16671 and P07996	Pubmed	SVM Score :0.0
We show that one peptide CSVTCG , which represents the CD 36 binding region of TSP 1 , stimulates resorption in a fashion similar to the intact molecule , while the peptides RGDS , RFYVVMWK , and RFYVVM , representing other cell binding domains of TSP , have no effect on resorption . ^^^ These studies suggest that CD 36 is involved in regulation of resorption by osteoclasts and is the receptor responsible for the resorption promoting effects of TSP 1 . . ^^^
Interacting proteins: P16671 and P07996	Pubmed	SVM Score :0.0
Biochemical analyses show expression of TSP 1 in human cartilage , but its cellular source as well as the presence of its main surface receptors CD 36 and CD 51 in normal and osteoarthritic cartilage remain unknown . ^^^ METHODS : Radioactive in situ hybridisations with an RNA probe that encodes TSP 1 combined with immunostaining were carried out to investigate the expression patterns of TSP 1 , CD 36 , and CD 51 in seven normal and 23 osteoarthritic human cartilage samples . ^^^ CONCLUSION : The cellular source of TSP 1 in normal cartilage is mainly mid zone chondrocytes , which also express CD 36 . ^^^
Interacting proteins: P16671 and P07996	Pubmed	SVM Score :0.0
The TSP 1 / L TGF beta 1 complex was associated with CD 36 , a receptor for TSP 1 . ^^^ The association of TSP 1 / L TGF beta 1 to CD 36 was critical for plasmin mediated release of mature TGF beta 1 . ^^^
Interacting proteins: P16671 and P07996	Pubmed	SVM Score :0.0
RESULTS : Comparing with normal donors , the expression of CD42a in cancer patients decreased , and that of TSP and CD 36 increased . ^^^
Interacting proteins: P16671 and P07996	Pubmed	SVM Score :0.0
Thrombospondin 1 ( TSP 1 ) can inhibit angiogenesis by interacting with endothelial cell CD 36 or proteoglycan receptors . ^^^ In solution , both intact TSP 1 and the alpha3beta1 integrin binding peptide from TSP 1 inhibit proliferation of sparse endothelial cell cultures independent of their CD 36 expression . ^^^
Interacting proteins: P16671 and P07996	Pubmed	SVM Score :0.0
Thrombospondin ( TSP ) , which is secreted from alpha granules of activated platelets , binds to its surface receptor ( CD 36 ) in the presence of Ca2+ . ^^^ OBJECTIVES : We monitored how the modulation of intraplatelet Ca2+ affects TSP binding to CD 36 on platelets from healthy donors and patients with type 2 diabetes mellitus . ^^^ METHODS : Whole blood cytometry was used to monitor TSP release / binding and CD 36 presentation in platelets from 28 type 2 patients and 33 healthy donors . ^^^ Results : No significant changes in TSP and CD 36 levels were revealed between the groups in circulating platelets and TRAP , collagen or thrombin activated platelets . ^^^ Conclusion : Both the release of TSP and CD 36 presentation are under the influence of agents modulating intracellular Ca2+ . ^^^
Interacting proteins: P16671 and P07996	Pubmed	SVM Score :0.0
TSP 1 and TSP 2 have anti angiogenic activity , which , at least for TSP 1 , involves interaction with the microvascular endothelial cell receptor CD 36 . ^^^
Interacting proteins: P16671 and P07996	Pubmed	SVM Score :0.0
The enhanced IL 6 release by TSP 1 was inhibited by anti CD 36 antibody or antibody against the sequence of the binding site to CD 36 in the type 1 repeats of TSP 1 . ^^^ Conversely , the decrease in IL 10 release by TSP 1 was strengthened by the blocking of the interaction between CD 36 and TSP 1 . ^^^ Together , the present findings suggest that TSP 1 enhances IL 6 release from macrophages by interaction with CD 36 , whereas IL 10 release is regulated by the balance between the enhancing effect of TSP 1 via CD 36 and the suppressive effect by TSP 1 activated TGF beta1 . . ^^^
Interacting proteins: P16671 and P07996	Pubmed	SVM Score :0.0
TSP 1 and its potential membrane receptors , e . g . alphaIIbbeta 3 integrin , CD 36 and CD 47 , concomitantly associated with the actin cytoskeleton . ^^^ However , studies on platelets from a patient with type 1 Glanzmann ' s thrombasthenia lacking alphaIIbbeta 3 and another with barely detectable CD 36 showed normal TSP 1 surface expression and association with the actin cytoskeleton . ^^^ Finally , assembly of signalling complexes , as observed through translocation of tyrosine phosphorylated proteins and kinases to the actin cytoskeleton , was found to occur in concert with cytoskeletal association of TSP 1 , in control platelets as well as in thrombasthenic and CD 36 deficient platelets . ^^^ Our results imply a role for the actin cytoskeleton in the membrane surface expression process of TSP 1 molecules and suggest a possible coupling of TSP 1 receptors to signalling events occurring independently of alphaIIbbeta 3 or CD 36 . ^^^
Interacting proteins: P16671 and P07996	Pubmed	SVM Score :0.0
In contrast , the augmented cell aggregation by the 70 kDa fragment was hampered by anti CD 36 mAb or antibody against the CD 36 binding site of TSP 1 . ^^^ We therefore propose that TSP 1 may regulate LFA 1 / ICAM 1 mediated cell adhesion of monocytes / macrophages by either the inhibitory effect through CD 47 or the promoting effect through CD 36 depending on which domain / fragment is functional in a given biological setting . . ^^^
Interacting proteins: P16671 and P07996	Pubmed	SVM Score :0.0
Immunohistochemistry and Western blot analyses demonstrated that TSP 1 protein and its receptor , CD 36 , were present in the early antral phase and were localized primarily to the granulosa cells of antral follicles . ^^^ The temporal pattern of expression of TSP 1 , 2 , and CD 36 , which mirrors the active phases of angiogenesis in this experimental model , is compatible with a role for these proteins in the control of ovarian vascularization . . ^^^
Interacting proteins: P16671 and P07996	Pubmed	SVM Score :0.0
RBC platelet adhesion was reduced in half by antibodies against CD 36 or GPIb , but not by antibodies against GPIIb / IIIa , von Willebrand factor ( VWF ) , thrombospondin ( TSP ) , P selectin , beta ( 1 ) , alpha ( 5 ) , or CD 47 . ^^^ RBC neutrophil adhesion was diminished by anti CD11b ( 76 % ) , anti RBC Landsteiner Wiener ( LW ) ( ICAM 4 ; 40 % ) , or by EDTA ( > 80 % ) , but not by soluble fibrinogen or antibodies against CD11a , CD11c , CD 36 , TSP , beta ( 1 ) , alpha ( 5 ) , or CD 47 . ^^^
Interacting proteins: P16671 and P07996	Pubmed	SVM Score :0.0
Concomitant with these results , we found that the endothelial TSP 1 receptor CD 36 was mostly absent from cutaneous lymphatic vessels . ^^^
Interacting proteins: P16671 and P07996	Pubmed	SVM Score :0.0
CONCLUSION : The binding of 260 320 kDa PfEMP 1 proteins expressed on PE from cerebral malaria patients to diverse receptor molecules on the endothelial cell ( EC ) of the cerebral microvessels such as CD 36 , thrombospondin ( TSP ) , intercellular adhesion molecule 1 ( ICAM 1 ) , vascular cell adhesion molecule 1 ( VCAM 1 ) , endothelial leukocyte adhesion molecule 1 ( ELAM 1 ) and chondroitin sulfate A ( CSA ) might be the molecular basis for the pathogenesis of cerebral malaria . . ^^^
Interacting proteins: P16671 and P07996	Pubmed	SVM Score :0.0
Expression of TSP 1 and its receptor CD 36 was increased on the surface of apoptotic fibroblasts . ^^^ By chemical cross linking and immunoprecipitation we show that TSP 1 and CD 36 were directly associated . ^^^ Blockade of either CD 36 or TSP 1 on apoptotic fibroblasts inhibited phagocytosis . ^^^ Blockade of alpha 5 beta 3 integrins as well as CD 36 and TSP 1 on macrophages inhibited phagocytosis . ^^^
Interacting proteins: P16671 and P07996	Pubmed	SVM Score :0.0
Given that anti CD 47 treatment of platelets did not further decrease the adhesion of anti CD 36 treated platelets and CD 36 is a TSP 1 receptor , it appears that CD36 / TSP 1 interaction could trigger the CD 47 dependent pathway . ^^^
Interacting proteins: P16671 and P07996	Pubmed	SVM Score :0.0
BACKGROUND : CD 36 is a suspected facilitator of long chain fatty acid transport and as a thrombospondin ( TSP ) receptor , thereby being implicated in cell proliferation , angiogenesis and tumor metastasis . ^^^ The human amelanotic melanoma cell line , C 32 , is known to express CD 36 and has been as a model for studying TSP binding . ^^^
Interacting proteins: P16671 and P07996	Pubmed	SVM Score :0.0
A scavenger receptor , CD 36 , recognizes the active sequences in the type 1 repeats , and is required for the anti angiogenic effects of TSP 1 in the corneal neovascularization assay . ^^^ Binding of TSP 1 to CD 36 has been shown to activate apoptosis by inducing p 38 and Jun N terminal kinase , members of the mitogen activated protein kinase superfamily , and subsequently the cell surface expression of FasL . ^^^
Interacting proteins: P16671 and P07996	Pubmed	SVM Score :0.0
CD 36 is the cellular receptor for TSP 1 on microvascular endothelium and is necessary for its anti angiogenic activity . ^^^ Structure function analyses have determined that binding of TSP 1 to CD 36 is mediated by interaction of the TSR 1 domain of TSP with a conserved domain called CLESH 1 in CD 36 . ^^^ Several in vivo models support the role of the TSP / CD36 system in angiogenesis and tumor growth and provide evidence that the CD 36 antiangiogenic pathway offers attractive therapeutic targets . . ^^^
Interacting proteins: P16671 and P07996	Pubmed	SVM Score :0.0
The endogenous TSP produced during early DC activation negatively regulates IL 12 , TNF alpha , and IL 10 release through its interactions with CD 47 and CD 36 . ^^^
Interacting proteins: P16671 and P07996	Pubmed	SVM Score :0.0
Thrombospondin 1 ( TSP 1 ) is a naturally occurring anti angiogenic compound that induces apoptosis of endothelial and cancer cells via its receptor CD 36 . ^^^ The objectives of our study were to investigate the in vitro effects of TSP 1 on the apoptosis of primary human leukemia cells as well as leukemia cell lines and the possible mechanism involving CD 36 . ^^^ Our results demonstrated that TSP 1 induced apoptosis in CD 36 positive cell lines CHRF 288 11 , Meg 01 and HL 60 , but not CD 36 negative K 562 , at a dose dependent manner as demonstrated by DNA ladder formation , Annexin 5 and propidium iodide ( PI ) stainings . ^^^ The addition of anti CD 36 antibody FA 6 152 or thrombopoietin ( TPO ) significantly nullified the effects of TSP 1 . ^^^ Responses of 2 CD 36 positive primary AML samples to TSP 1 and FA 6 152 were similar with those of leukemia cell lines . ^^^
Interacting proteins: P16671 and P07996	Pubmed	SVM Score :0.0
We found a pronounced induction of the TSP 1 receptor CD 36 in CD 31 positive vessels on day 8 after UVB irradiation , associated with vascular endothelial cell apoptosis . ^^^
Interacting proteins: P16671 and P07996	Pubmed	SVM Score :0.0
MATERIALS AND METHODS : The expression of TSP 1 , TSP 2 and CD 36 receptor was assessed in 73 tissue specimens using immunohistochemistry ; specimens were from 32 patients with BPH , seven with prostatic intraepithelial neoplasia ( PIN ) and 34 with cancer . ^^^ All 73 patients were positive for TSP receptor CD 36 and negative for TSP 2 . ^^^
Interacting proteins: P16671 and P07996	Pubmed	SVM Score :0.0
Although antibody A4 . 1 is also an antagonist of CD 36 binding to TSP 1 , these data suggest that some biological activities of A4 . 1 result from antagonism of these novel beta ( 1 ) integrin binding sites . . ^^^
Interacting proteins: P16671 and P07996	Pubmed	SVM Score :0.0
The role of the erythrocyte anion exchanger , band 3 protein ( AE 1 ) , in the adhesion of Plasmodium falciparum infected erythrocytes to CD 36 and thrombospondin ( TSP ) was studied . ^^^ Modification of lysine 539 by diisothiocyanostilbene sulfonic acid ( DIDS ) resulted in a significant reduction in the adhesive properties of parasitized erythrocytes for CD 36 , but not TSP , whereas treatment with fluorescein 5 maleimide , which modifies lysine 430 , was without effect on both TSP and CD 36 binding . ^^^
Interacting proteins: P16671 and P07996	Pubmed	SVM Score :0.0
TSP 1 acts by inducing endothelial cell apoptosis via signaling cascade initiated at CD 36 , a TSP 1 antiangiogenic receptor . ^^^ Here , we show that the ligands of nuclear receptor peroxisome proliferator activated receptor gamma ( PPARgamma ) , 15 deoxy delta ( 12 , 14 ) prostaglandin J 2 , troglitazone , and rosiglitazone increased PPARgamma and CD 36 expression in endothelial cells and improved the efficacy of TSP 1 and ABT 510 in a CD 36 dependent manner . ^^^
Interacting proteins: P16671 and P07996	Pubmed	SVM Score :0.0
Moreover , in the presence of antibodies to TSP 1 and CD 36 , the receptor for TSP 1 , the adenosine A2A receptor agonists stimulated no increase in vascular tube formation . ^^^
Interacting proteins: P16671 and P07996	Pubmed	SVM Score :0.0
The tumor suppressive action of TSP 1 , endostatin , and tumstatin correlates with expression of CD 36 receptor , alpha5beta1 integrin , and alphavbeta 3 integrin on proliferating endothelial cells , respectively . ^^^
Interacting proteins: P16671 and P07996	Pubmed	SVM Score :0.0
We have previously shown that the antiangiogenic activity of TSP 1 is mediated by the interaction of the type 1 repeats ( TSR ) with the receptor CD 36 , although other domains of TSP 1 have also been implicated . ^^^ We now show that the antiangiogenic activity of TSP 2 , which contains three TSRs but , unlike TSP 1 , lacks the capacity to activate TGF beta , is similarly dependent on CD 36 . ^^^ Using the corneal pocket assay we found that TSP 2 did not inhibit bFGF induced angiogenesis in CD 36 null mice . ^^^ We then demonstrated that ( 125 ) [ 1 ] TSP 2 bound to murine macrophages and that binding was diminished by 70 % by anti CD 36 antibody or by using cells from CD 36 null animals . ^^^
Interacting proteins: P16671 and P07996	Pubmed	SVM Score :0.0
Two conclusions can be made regarding the mechanisms of action of these inhibitors : ( 1 ) Several of these inhibitors appear to mediate their antiangiogenic effect through multiple protein protein interactions that inhibit the function of proangiogenic molecules rather than through a specific receptor mediated signaling event , and ( 2 ) TSP 1 and TSP 2 appear to mediate their antiangiogenic effect , at least in part , through a specific receptor , CD 36 , which initiates the antiangiogenic signal . ^^^
Interacting proteins: P16671 and P07996	Pubmed	SVM Score :0.0
TSP 1 and TSP 1 derived synthetic peptides based on the CD 36 and CD 47 binding sites of TSPs increased thymidine incorporation into bovine erythroid cells of fetal liver . ^^^
Interacting proteins: P16671 and P07996	Pubmed	SVM Score :0.0
Thrombospondin 1 ( TSP 1 ) , a natural inhibitor of angiogenesis , acts directly on endothelial cells ( EC ) via CD 36 to inhibit their migration and morphogenesis induced by basic fibroblast growth factor . ^^^ Here we show that CD 36 triggered by TSP 1 inhibits in vitro angiogenesis stimulated by vascular endothelial growth factor A ( VEGF A ) . ^^^ To demonstrate that the TSP 1 inhibitory signal was mediated by CD 36 , we transduced CD 36 in CD 36 deficient endothelial cells . ^^^ Both TSP 1 and the agonist anti CD 36 mAb SMO , which mimics TSP 1 activity , reduced the VEGF A 165 induced migration and sprouting of CD 36 ECs . ^^^ Our results indicate that C 464 , R 467 , and K 469 of CD 36 are required for the inhibitory activity of TSP 1 . ^^^
Interacting proteins: P16671 and P07996	Pubmed	SVM Score :0.0
The present study was therefore designed to evaluate the expression of thrombospondin 1 and 2 ( TSP 1 , TSP 2 ) , TSP receptors CD 36 and CD 47 , and matrix metalloproteases 2 and 9 ( MMP , MMP 9 ) in a rat prostate cancer model . ^^^ By using immunohistochemistry , Western blot , and real time PCR the expression patterns of TSP 1 , TSP 2 , CD 36 , CD 47 , MMP 2 , and MMP 9 were investigated in normal rat prostate tissue and five malignant Dunning sublines tissue . ^^^ However , there was no difference in expression of TSP 2 and CD 36 mRNA in these samples . ^^^
Interacting proteins: P16671 and P07996	Pubmed	SVM Score :0.0
NO stimulated endothelial cell responses are inhibited by recombinant type 1 repeats of TSP 1 and a CD 36 agonist antibody but not by the N terminal portion of TSP 1 , suggesting that CD 36 or a related receptor mediates these effects . ^^^
Interacting proteins: P16671 and P07996	Pubmed	SVM Score :0.0
CD 36 , one of TSP ' s main receptors , was also analysed . ^^^ The expression pattern of TSP 1 , TSP 2 and CD 36 was investigated using immunohistochemistry and Western blot analyses . ^^^
Interacting proteins: P16671 and P07996	Pubmed	SVM Score :0.0
Binding of thrombospondin 1 ( TSP 1 ) to the CD 36 receptor inhibits angiogenesis and induces apoptosis in endothelial cells ( EC ) . ^^^ In this study we analyzed the shear stress dependent expression of TSP 1 and CD 36 in endothelial cells in vitro and in vivo to reveal its putative role in the blood flow induced remodelling of vascular networks . ^^^ Shear stress downregulated TSP 1 and CD 36 expression in vitro in a force and time dependent manner sustained for at least 72 h and reversible by restoration of no flow conditions . ^^^ In vivo , shear stress driven increase of C / F in prazosin fed mice was associated with reduced expression of TSP 1 and CD 36 in ECF , while TSP 1 expression in WMH was increased . ^^^
Interacting proteins: P16671 and P07996	Pubmed	SVM Score :0.0
While TSP treated APCs matched TGFbeta treated APCs in their functional ability to induce systemic tolerance , a deficiency of TSP 1 or its receptor CD 36 prevented APCs from becoming tolerogenic in response to TGFbeta . ^^^ Both TGFbeta treated TSP 1 null and CD 36 knockout APCs failed to inhibit IL 12 secretion . ^^^
Interacting proteins: P16671 and P07996	Pubmed	SVM Score :0.0
Mice subjected to CNV were treated topically ( thrice daily ) with CD 36 functionally neutralizing antibodies against the oxidized low density lipoprotein ( oxLDL ) and thrombospondin ( TSP ) 1 sites ( clones JC63 . 1 and FA 6 152 , respectively ) . ^^^
Interacting proteins: P16671 and P07996	Pubmed	SVM Score :0.0
Furthermore , three patients with ET demonstrated increased levels of platelet surface glycoprotein 4 ( GP 4 ) , the putative receptor for TSP in the plasma membrane . ^^^ The results suggest a possible link between the increased number of plasma membrane GP 4 molecules , the spontaneous expression of TSP on the platelet surface and platelet hyperaggregability in some ET patients . ^^^ The levels of plasma membrane GP 4 and platelet surface associated TSP tended to be normalized during alpha interferon treatment , whereas the presence of an altered form of TSP persisted . ^^^
Interacting proteins: P16671 and P07996	Pubmed	SVM Score :0.0
METHODS : Glycoprotein 4 ( GP 4 ) and thrombospondin ( TSP ) levels on the membrane surface of the stationary platelet or platelet activated by thrombin ( 0 . 05 U / ml , 0 . 1 U / ml , 0 . 5 U / ml , 1 . 0 U / ml ) in 16 patients with MS were measured with flow cytometric method and compared with those of the healthy ( 14 cases ) . ^^^ RESULTS : The GP 4 level of stationary platelet , the GP 4 and TSP levels of activated platelet in MS patients were higher than those in the healthy significantly ( P < 0 . 01 ) , while the TSP level of stationary platelet was not different between the patients and the healthy ( P > 0 . 05 ) . ^^^ The GP 4 redistribution on the activated platelet surface was apparently inhibited by dauricine ( 50 mumol / L , P < 0 . 05 , P < 0 . 01 ) and the release of TSP from intracellular alpha granules was inhibited by dauricine only in the activated platelets induced by thrombin of low concentration ( 0 . 05 U / ml and 0 . 1 U / ml , P < 0 . 05 , P < 0 . 01 ) , inhibiting effect was not found in those activated with high concentration of thrombin . ^^^
Interacting proteins: P16671 and P07996	Pubmed	SVM Score :0.0
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Interacting proteins: P16671 and P07996	Pubmed	SVM Score :0.0
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Interacting proteins: P16671 and P07996	Pubmed	SVM Score :0.0
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Interacting proteins: P16671 and P07996	Pubmed	SVM Score :0.0
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Interacting proteins: P16671 and P07996	Pubmed	SVM Score :0.0
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Interacting proteins: P16671 and P07996	Pubmed	SVM Score :0.0
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Interacting proteins: P16671 and P07996	Pubmed	SVM Score :0.0
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Interacting proteins: P16671 and P07996	Pubmed	SVM Score :0.0
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Interacting proteins: P16671 and P07996	Pubmed	SVM Score :0.0
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Interacting proteins: P16671 and P07996	Pubmed	SVM Score :0.0
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Interacting proteins: P16671 and P07996	Pubmed	SVM Score :0.0
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Interacting proteins: P16671 and P07996	Pubmed	SVM Score :0.0
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Interacting proteins: P16671 and P07996	Pubmed	SVM Score :0.0
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Interacting proteins: P16671 and P07996	Pubmed	SVM Score :0.0
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Interacting proteins: P16671 and P07996	Pubmed	SVM Score :0.0
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Interacting proteins: P16671 and P07996	Pubmed	SVM Score :0.0
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Interacting proteins: P16671 and P07996	Pubmed	SVM Score :0.0
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Interacting proteins: P16671 and P07996	Pubmed	SVM Score :0.0
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