Pubmed abstracts for Protein-Protein Interaction search result :

Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.6844705
To study the activation of human 72 kDa gelatinase , and its relation to tissue inhibitor of metalloproteinases 2 ( TIMP 2 ) , we purified human 72 kDa progelatinase both as a complex with TIMP 2 and as a free proteinase . 0.6844705^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.50222888
Synthesis and refolding of human TIMP 2 from E . coli , with specific activity for MMP 2 . 0.50222888^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.76364804
Thrombin cleaved pro MMP 2 within 4 hours , even when the gelatinase was bound with its inhibitor , TIMP 2 . 0.76364804^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.89846124
CONCLUSIONS : In neuroblastoma , stromal tissues are actively involved in the complex interaction between MMP 2 and TIMP 2 in extracellular matrix degradation during tumor progression , and TIMP 2 expression is inversely correlated with the corresponding MMP 2 . 0.89846124^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.97745546
Using immunoaffinity techniques , endogenous TIMP 2 in the inflamed lung was shown to exist as a complex with 72 and 92 kDa metalloproteinases ( MMP 2 and MMP 9 ) . 0.97745546^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.53927361
Determination of imbalance between MMP 2 and TIMP 2 in human neuroblastoma by reverse transcription polymerase chain reaction and its correlation with tumor progression . 0.53927361^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.91422738
MT 1 MMP bound TIMP 2 has been shown to function as a receptor for secreted pro MMP 2 , resulting in the formation of a trimolecular complex . 0.91422738^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.59740736
TIMP 2 , a specific inhibitor for MMP 2 , was significantly lower in the borderline category than in the adenoma category . 0.59740736^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
In the present investigation , we studied the effect of basic fibroblast growth factor ( bFGF ) on collagen , matrix metalloproteinase 2 ( MMP 2 ) , and tissue metalloproteinase inhibitor 2 ( TIMP 2 ) levels in normal and carcinoma lung tissue fibroblast cultures . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Expression of the mammalian collagenase ( MMP 1 ) , 72 kD gelatinase / type 4 collagenase ( MMP 2 ) , stromelysin ( MMP 3 ) , 92 kD gelatinase / type 4 collagenase ( MMP 9 ) , and tissue inhibitors of metalloproteinases ( TIMP 1 and TIMP 2 ) was assessed by zymography and Northern blot analysis . ^^^ MMP 2 and TIMP 2 activities were refractory to TPA , IL 1 and TNF alpha treatment in most of the cell lines . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
The human 72 kDa type 4 collagenase ( matrix metalloproteinase 2 ) and its proteinase inhibitor , tissue inhibitor of metalloproteinases 2 ( TIMP 2 ) , are produced as a proenzyme inhibitor complex by numerous cell lines . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
The 72 kDa gelatinase / type 4 collagenase , a metalloproteinase thought to play a role in metastasis and in angiogenesis , forms a noncovalent stoichiometric complex with the tissue inhibitor of metalloproteinase 2 ( TIMP 2 ) , a potent inhibitor of enzyme activity . ^^^ To define the regions of the 72 kDa gelatinase responsible for TIMP 2 binding , a series of NH 2 and COOH terminal deletions of the enzyme were constructed using the polymerase chain reaction technique . ^^^ In addition , a truncated version of the 72 kDa gelatinase lacking the NH 2 terminal 78 amino acids ( delta 1 78 ) of the proenzyme retained the ability to bind TIMP 2 . ^^^ These data suggest that both the active site and the COOH terminal tail of the 72 kDa gelatinase independently and cooperatively participate in TIMP 2 binding . . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Stromal expression of 72 kda type 4 collagenase ( MMP 2 ) and TIMP 2 mRNAs in colorectal neoplasia . ^^^ We undertook an in situ hybridization study to localize the mRNAs for the 72 kda type 4 collagenase ( MMP 2 ) and its specific inhibitor ( TIMP 2 ) in 12 colorectal carcinomas , 3 adenomas , and 4 uninvolved resection margins to see how their distributions correlated with that of the reported distribution of MMP 2 protein . ^^^ Labeling for MMP 2 and TIMP 2 mRNAs was detectable in 10 of 12 carcinomas and in 2 of 3 adenomas . ^^^ Unexpectedly , we found much stronger signals for MMP 2 and TIMP 2 mRNAs within the mesenchymal cells in the desmoplastic stroma , of endothelial and / or ( myo ) fibroblastic nature , rather than in tumor epithelial cells in which localization of MMP 2 was anticipated . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
In this report we have used sensitive enzyme linked immunoassays to measure MMP 2 , MMP 9 , TIMP 1 and TIMP 2 levels in eight human lung cancer cell lines which were characterized for biological behavior in nude mice . ^^^ TIMP 2 secretion , which exceeded MMP 2 secretion for all cell lines , did not correlate with metastatic potential . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
We therefore examined the effects of 10 nM estradiol and / or 10 nM progestin R 5020 on the expression of 2 non integrin laminin binding proteins , the 67 kDa laminin receptor ( 67LR ) and HLBP 31 as well as the 72 kDa type 4 collagenase ( MMP 2 ) and its inhibitor , TIMP 2 , in steroid receptor positive ( T47D and MCF 7 ) and negative ( MDA MB 231 ) human breast cancer cells . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
In assays measuring the relative inhibitory capability of TIMP 1 and TIMP 2 against autoactivated 72 kDa gelatinase , which consists of two major active peptides and several inactive fragments , TIMP 2 was more effective than TIMP 1 . ^^^ The isolated 42 . 5 kDa active fragment that formed as a result of the autoactivation of 72 kDa gelatinase showed the greatest preference for TIMP 2 ; at half maximal inhibition , TIMP 2 was greater than 10 fold more effective than TIMP 1 . ^^^ TIMP 2 was also greater than 2 fold more effective than TIMP 1 at inhibiting 72 kDa gelatinase TIMP 2 complexes activated with 4 aminophenylmercuric acetate , and greater than 7 fold more effective than TIMP 1 at inhibiting 92 kDa gelatinase activated with 4 aminophenylmercuric acetate . ^^^ The ratios of 125I TIMP 2 / 125I TIMP 1 binding to 92 kDa gelatinase , autoactivated 72 kDa gelatinase , and 42 . 5 kDa fragment were 4 . 4 , 10 , and 33 , respectively . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
We have identified a binding site for tissue inhibitors of metalloproteinases 2 ( TIMP 2 ) on human 72 kDa gelatinase that is distinct from the active site . 72 kDa progelatinase is found in a complex with TIMP 2 in the medium of cultured cells and can be activated with organomercurial compounds to yield a gelatinolytic proteinase that remains bound to TIMP 2 . ^^^ Saturation binding kinetics for the active site of 72 kDa gelatinase were measured in pools of the 43 kDa active fragment that results from the autoactivation of 72 kDa progelatinase ; this fragment has no carboxyl terminal TIMP 2 binding capability . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
It is postulated that these regions are responsible for the unique ability of TIMP 2 to bind to the latent form of the 72 kDa type 4 collagenase . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
The TIMP 2 inhibitor interacts with the 72 kDa type 4 collagenase in preference to the 92 kDa type 4 collagenase that forms a complex exclusively with TIMP . ^^^ The 72 kDa type 4 collagenase TIMP 2 complex can be activated with organomercurials to yield a catalytically competent enzyme . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Here we demonstrate that two specific inhibitors of MMP 2 , TIMP 2 and a peptide from the MMP 2 propeptide region ( peptide 74 ) , inhibit endothelial cell invasion induced by AIDS KS cell supernatants . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
These effects of TIMP 2 appear to be mediated by inhibition of gelatinase A activity . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Using semi quantitative reverse transcription and the polymerase chain reaction , mRNA levels of stromelysin , gelatinase B and TIMP 1 are shown to increase after laser treatment , while gelatinase A and TIMP 2 remain relatively constant . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Gelatinase A / TIMP 2 imbalance in lymph node positive breast carcinomas , as measured by RT PCR . ^^^ Over production of gelatinase A ( MMP 2 ) or under production of its inhibitor ( TIMP 2 ) may result in the matrix degradation crucial for metastasis , and early evaluation of their expression in primary tumor would offer important prognostic informations . ^^^ RT PCR amplicons of MMP 2 and TIMP 2 mRNA from tissue biopsies of 13 breast carcinomas and one fibrocystic mastopathy were quantitated . ^^^ In comparison with their normal tissue counterparts , their expression trends were not uniform : in some cases MMP 2 increased in the tumor without changes in TIMP 2 , in others TIMP 2 expression also increased , although to a lesser extent than MMP 2 ; only in 2 cases was it slightly lower in the tumor tissue . ^^^ Results suggest that evaluation of MMP 2 / TIMP 2 mRNA balance may constitute an early prognostic approach , which may also be more reliable concerning cancer aggressiveness as compared with the MMP 2 alone , and that boosting TIMP 2 expression may be a therapeutic strategy to prevent metastasis . . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
In five human glioma cell lines , gelatinase A , TIMP 1 , and TIMP 2 genes were constitutively expressed in alll cell lines : the matrilysin gene in three cell lines ; the stromelysin gene in two cell lines ; and the interstitial collagenase gene in one cell line . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Da type 4 collagenase and TIMP 3 , but not 72 kDa type 4 collagenase or TIMP 1 or TIMP 2 , are highly expressed during mouse embryo implantation . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
To study the role of MMPs in the progression of human breast carcinomas , we examined production and tissue localization of MMP 1 , MMP 2 , MMP 3 , MMP 9 and their common inhibitors , tissue inhibitors of metalloproteinases ( TIMP 1 and TIMP 2 ) . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
In the control brain tissues , mRNA expression was high for TIMP 2 , low for gelatinase A and TIMP 1 , and undetectable for gelatinase B , interstitial collagenase , stromelysin and matrilysin . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Steady state levels of transcripts encoding extracellular matrix proteins , MMP 2 ( 72 kDa collagenase 4 ) , MMP 9 ( 92 kDa collagenase type 4 ) , TIMP 1 and TIMP 2 were elevated in the majority of pancreatic cancer tissue samples as compared to control pancreatic tissue . ^^^ In situ hybridization studies localized transcripts coding for collagens type 1 and 3 to spindle shaped stromal cells , whereas transcripts for MMP 2 , MMP 9 , TIMP 1 and TIMP 2 were found in both stromal and tumor cells . ^^^ However , MMP 2 transcripts appeared to be more abundant in stromal cells , TIMP 1 and TIMP 2 transcripts were evenly distributed over tumor and stromal cells and relatively more MMP 9 transcripts were found in tumor cells . ^^^ We conclude that , in human pancreatic cancer , MMP 2 , MMP 9 , TIMP 1 and TIMP 2 may be involved in processes leading to the strong desmoplastic reaction observed in these tumors . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Alteration in expression of MMP 2 or its inhibitor , the tissue inhibitor of metalloproteinases type two ( TIMP 2 ) , could increase extracellular matrix degradation and lead to aneurysm formation . ^^^ The purpose of this study is ( 1 ) to measure relative tissue levels of MMP 2 and TIMP 2 mRNA in aneurysmal , occlusive , and normal human infrarenal aorta ; ( 2 ) to test for expression by cultured aneurysmal and normal vascular smooth muscle cells ( VSMCs ) ; and ( 3 ) to identify , in situ , the cells responsible for mRNA production within aneurysmal , occlusive , and normal aortic wall . ^^^ Signals for MMP 2 and TIMP 2 were normalized to alpha tubulin . ^^^ In situ hybridization with digoxigenin RNA probes localized cells responsible for MMP 2 and TIMP 2 mRNA production in histologic sections of aneurysmal ( n = 7 ) , occlusive ( n = 4 ) , and normal ( n = 3 ) aorta . ^^^ Cultured aneurysmal and normal VSMCs constitutively expressed both MMP 2 and TIMP 2 . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Tissue inhibitor of metalloproteinase 2 ( TIMP 2 ) , a protease inhibitor that binds to the latent and active forms of 72 kDa type 4 collagenase ( gelatinase A ) , was found to inhibit the in vitro proliferation of human microvascular endothelial ( HME ) cells stimulated with bFGF and 5 % serum . ^^^ The inhibition of cell proliferation observed with TIMP 2 was not mimicked by addition to the culture medium of BB 94 , a general matrix metalloproteinase inhibitor , nor antibodies to the 72 kDa type 4 collagenase . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Western blot studies further suggested that jaw cyst gelatinases were only in part complexed with and thus inhibited by TIMP 1 or TIMP 2 , suggesting that both MMP 9 and MMP 2 may participate in cyst expansion . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Cell surface binding of TIMP 2 and pro MMP 2 / TIMP 2 complex . ^^^ TIMP 2 is found both free and in a 1 : 1 stoichiometric complex with the pro enzyme form of MMP 2 ( pro MMP 2 / TIMP 2 complex ) . ^^^ Specific binding of MMP 2 alone to HT 1080 cells was not observed ; however , pro MMP 2 / TIMP 2 complex was capable of binding to the surface of HT 1080 cells in a TIMP 2 dependent manner . ^^^ These results suggest that rTIMP 2 alone binds directly to the cell surface of HT 1080 and MCF 7 cell lines , and TIMP 2 is capable of localizing MMP 2 to the surface of HT 1080 cells via interaction with a specific binding site . . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
We have examined the expression of 72 kDa gelatinase / type 4 collagenase or matrix metalloproteinase 2 ( MMP 2 ) and its inhibitor , tissue inhibitor of metalloproteinase 2 ( TIMP 2 ) , in various cell lines derived from paediatric tumours . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Altered expression of matrix metalloproteinase 2 , TIMP , and TIMP 2 in obstructive nephropathy . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
The removal of the propeptide of the proMMP 2 * bound to TIMP 2 was also observed by MMP 3 ( stromelysin 1 ) , but not by MMP 1 ( interstitial collagenase ) . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
No gross alterations in the expression of MMP 1 , MMP 3 , TIMP 1 and TIMP 2 transcripts were detected in these cell lines . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
We report herein that organomercurial or plasma membrane activated M ( r ) 72 , 000 gelatinase A activates progelatinase B to an M ( r ) 82 , 000 active form in a process inhibited by tissue inhibitor of metalloproteinase ( TIMP ) 1 and TIMP 2 . ^^^ Progelatinase B activation was accomplished by the two active species of gelatinase A , the M ( r ) 62 , 000 and M ( r ) 45 , 000 forms , generated after plasma membrane or organomercurial activation of TIMP 2 free progelatinase A . ^^^ The M ( r ) 45 , 000 species of gelatinase A lacks both the NH 2 terminal profragment and the COOH terminal domain known to play a role in plasma membrane activation and the regulation of TIMP 2 inhibition . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
The protein amounts of TIMP 1 , TIMP 2 , and MMP 2 secreted into the medium from HT 1080 cells treated with 1 mM 8 bromo cAMP were 7 . 9 , 9 . 3 , and 8 . 5 fold higher than those secreted from untreated cells , respectively . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
In floating villi , gelatinase A and TIMP 1 were localized in the stromal compartment , whereas gelatinase B and TIMP 2 were codistributed in trophoblast cells . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
The purpose of this study was to correlate the presence of matrix metalloproteinase ( MMP ) 9 and MMP 2 and tissue inhibitors of metalloproteinase ( TIMP ) 1 and TIMP 2 mRNAs , detected in serial sections using the reverse transcriptase in situ PCR technique , with prognosis in 23 cases of cervical carcinoma . ^^^ The ratios of cancer and stromal cells expressing MMP 9 and MMP 2 to those expressing TIMP 1 and TIMP 2 were approximately 1 in those cancers with a good prognosis . ^^^ These data suggest that the balance of MMP 9 and MMP 2 to TIMP 1 and TIMP 2 expression is an essential factor in the aggressiveness of cervical cancer . . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
TIMP 2 was not detected in the inhibitory IPM fractions , consistent with the observed autoactivation of bovine IPM 72 kDa gelatinase . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Furthermore , plasma membrane fractions from these cells significantly enhanced the rate of cleavage of [ 14C ] gelatin 1 substrate by both MMP 2 tissue inhibitor of metalloproteinases 2 ( TIMP 2 ) complex ( 2 . 5 8 fold ) and TIMP 2 free MMP 2 ( 5 . 9 fold ) . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Activity of ternary gelatinase A TIMP 2 matrix metallo proteinase complexes . ^^^ This activity , however , amounts only to less than 10 % of that of free gelatinase A not complexed with TIMP 2 . ^^^ After activation with 4 aminophenylmercury acetate this ternary complex displays a more than tenfold proteolytic activity compared to activated gelatinase A TIMP 2 complex , thus reaching the activity of free gelatinase A . ^^^ These results suggest that gelatinase A TIMP 2 complex may be a matrix metalloproteinase of the ' second step ' : It starts its proteolytic attack after it has switched off the activity of other matrix metalloproteinases . . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Dot blots with polyclonal antibodies specific for MMP 2 , TIMP 1 and TIMP 2 revealed that the MMP 2 levels were high in subcutaneously grown PC 3 ML clones but low in 3 10 N . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Immunohistological staining of MMP 2 and MMP 9 , basal lamina collagen 4 and TIMP 2 were performed on frozen sections of 83 invasive breast carcinomas . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
The mRNA levels encoding for alpha 1 ( 1 ) , alpha 1 ( 3 ) , alpha 1 ( 4 ) collagen chains , laminin B 1 and B 2 chains , heparan sulfate proteoglycan ( HSPG ) , MMP 2 , TIMP 1 and TIMP 2 increased significantly as glomerulosclerosis progressed , whereas MMP 1 and MMP 3 mRNA levels were unchanged , and no MMP 9 mRNA was detected throughout the experiments . ^^^ In group 2 , the low protein diet reduced the prevalence of glomerulosclerosis and attenuated the increased mRNA expression for ECM components , MMP 2 , TIMP 1 and TIMP 2 in FGS glomeruli . ^^^ These data suggest that glomerulosclerosis may result from an imbalance among ECM components , MMPs and TIMPs and that a low protein diet attenuates the otherwise increased levels of mRNA for ECM components , MMP 2 , TIMP 1 and TIMP 2 in glomerulosclerosis . . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
The extra cellular matrix ( ECM ) related antigens , type 4 collagen , laminin , M ( r ) 68 , 000 laminin receptor ( LR ) , M ( r ) 72 , 000 type 4 collagenase ( MMP 2 ) , its inhibitor TIMP 2 , and alpha 2 macroglobulin expression have been immunohistochemically investigated in 100 cases of human gastric carcinoma with a 5 yr follow up . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
The pattern of TIMP 2 expression was similar to that of MMP 2 . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Steady state levels of transcripts encoding extracellular matrix proteins , MMP 2 ( 72 kDa collagenase 4 ) and TIMP 2 were enhanced in 7 out of 8 chronic pancreatitis tissue samples and showed a large degree of variation between individual patients . ^^^ The precise role of overexpression of MMP 2 and its inhibitor TIMP 2 will have to be elucidated in further studies . . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Expression of 72 kDa gelatinase and TIMP 2 in early and late passage human fibroblasts . ^^^ The expression and regulation of 72 kDa gelatinase and TIMP 2 were examined in cultures of early and late passage human fibroblasts . ^^^ Although Il 1 alpha had no significant effect on TIMP 2 mRNA or on expression of 72 kDa gelatinase , in either early or late passage cells , Il 1 alpha increased the level of a TIMP 2 72 kDa gelatinase complex . ^^^ Using monoclonal antibodies to TIMP 2 and to 72 kDa gelatinase we detected two forms of TIMP 2 . ^^^ Autoradiography in conjunction with Western analysis confirmed that in the late passage cell medium the newly synthesized 72 kDa gelatinase TIMP 2 complex was increased even though the expression of 72 kDa gelatinase did not change . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Immunohistochemical localization of matrix metalloproteinase 2 and its specific inhibitor TIMP 2 in neoplastic tissues with monoclonal antibodies . ^^^ Matrix metalloproteinase 2 ( MMP 2 ) , synthesized as a 631 amino acid proenzyme , is activated by cleavage of the first 80 amino acids and naturally inhibited by tissue inhibitor of metalloproteinase 2 ( TIMP 2 ) . ^^^ Matrix metalloproteinase 2 ( MMP 2 ) , synthesized as a 631 amino acid proenzyme , is activated by cleavage of the first 80 amino acids and naturally inhibited by tissue inhibitor of metalloproteinase 2 ( TIMP 2 ) . ^^^ We report here the production of MAbs against MMP 2 and TIMP 2 and their use in localizing the respective antigens on tumor tissues . ^^^ Besides their respective antigens , both MAbs also recognized the MMP 2 / TIMP 2 complex . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Immunoblot and gel filtration analyses showed that human sera contain several MMP 2 species in addition to proMMP 2 which exist in a complex form with TIMP 2 . . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Northern blots showed no significant changes in mRNA levels for MMP 1 , MMP 2 , MMP 3 , TIMP , or TIMP 2 . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
We review the functional domain structure of the matrix metalloprotease enzymes in general and specifically the interaction of metastasis associated gelatinase A ( 72 kDa type 4 collagenase ) with the tissue inhibitor of metalloproteases 2 ( TIMP 2 ) . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Synthetic oligonucleotide primers for four metalloproteinases ( MMP 1 , MMP 2 , MMP 9 , MMP 10 ) and their tissue inhibitors ( TIMP 1 , TIMP 2 ) were selected , synthesized , and optimized in the reverse transcriptase polymerase chain reaction ( RT PCR ) to study the qualitative profile of these enzymes and inhibitors in cultured human tumor cells and tumor tissues . ^^^ The results show MMP 1 and MMP 2 products in all three tumors , and MMP 2 detected in one of the three normal mucosa samples ; TIMP 2 expression was present in two of three patients and awaits quantitative assessment of RT PCR products . . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
We have investigated the expression and regulation of MMP 1 ( interstitial collagenase ) , MMP 2 ( gelatinase A ) , MMP 3 ( stromelysin 1 ) , MMP 7 ( matrilysin ) , MMP 9 ( gelatinase B ) and their inhibitors TIMP 1 and TIMP 2 in human umbilical vein , femoral vein and microvascular endothelial cells , and compared these data with those obtained with human synovial fibroblasts . ^^^ Non stimulated vein endothelial cells expressed the mRNAs for MMP 1 , MMP 2 , TIMP 1 and TIMP 2 . ^^^ On the other hand , MMP 2 and TIMP 2 were not affected or were affected in a variable way by TNF alpha and / or phorbol ester , suggesting a dissimilar regulation of these proteins . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
The system detected a free form of TIMP 2 and that complexed with active forms of matrix metalloproteinases ( MMPs ) giving a different sensitivity for each MMP but not TIMP 2 complexed with the precursor of 72 kDa gelatinase / type 4 collagenase ( MMP 2 ) . ^^^ Immunoreactivity analyses suggested that the precursor of MMP 2 in normal sera exists in a complexed form with TIMP 2 by interacting with the C terminal domain of TIMP 2 . . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
The activity of MMP 1 is regulated by tissue inhibitors of metalloproteinases , TIMP 1 or TIMP 2 , which form a non covalent complex with the active enzyme . ^^^ However , the recognition rate of MMP 1 is decreased to approximately 50 % and < 3 % for the MMP 1 TIMP 1 and MMP 1 TIMP 2 complex forms , respectively . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Plasma membrane dependent activation of the 72 kDa type 4 collagenase is prevented by complex formation with TIMP 2 . ^^^ Human 72 kDa type 4 collagenase ( 72T4Cl ) is secreted as a proenzyme that can form a specific stoichiometric complex with the tissue inhibitor of metalloproteases TIMP 2 via interaction with the carboxyl terminal domain of the enzyme . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
The purified tc TIMP 2 was separated into a 17 kDa peptide and a small peptide of about 2 . 5 kDa by reducing SDS PAGE and into four isoforms with pI 7 . 6 , 7 . 3 , 7 . 2 , and 6 . 8 by isoelectric focusing . tc TIMP 2 has essentially the same inhibitory activity as TIMP 2 toward gelatinase A , collagenase , stromelysin , and matrilysin . ^^^ Unlike TIMP 2 , however , tc TIMP 2 does not bind to the latent precursor fo gelatinase A . ^^^ These results suggest that proteolytic processing of TIMP 2 plays a role in the regulation of gelatinase A activity in the extracellular matrix . . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
We investigated the gene expressions of MMP 9 ( 92 kDa type 4 collagenase ) , MMP 2 ( 72 kDa type 4 collagenase ) , TIMP 1 and TIMP 2 in bladder cancers by Northern blot and slot blot hybridization . ^^^ The mRNA levels of MMP 2 , TIMP 1 and TIMP 2 increased in the cases with invasion and metastasis of bladder cancers . ^^^ The MMP 2 / TIMP 2 ratio increased as tumor invasion and metastasis progressed , suggesting that an imbalance in the MMP and TIMP ratio promote the invasion and metastasis of bladder cancers . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
The temporal pattern of 72 kDa gelatinase type A followed that of TIMP 2 in the decidua and ovary . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Whereas PCR analysis showed that mRNA was detected for each of the proteins , the immunolocalization study demonstrated that MMP 1 , MMP 2 , MMP 9 and TIMP 1 production was restricted to only a proportion of the tumour cells , with no evidence of MMP 3 or TIMP 2 synthesis . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Expression of human recombinant 72 kDa gelatinase and tissue inhibitor of metalloproteinase 2 ( TIMP 2 ) : characterization of complex and free enzyme . ^^^ To investigate 72 kDa gelatinase TIMP 2 interactions and to compare the activity of the complex versus that of the free enzyme , we have expressed and purified human 72 kDa progelatinase and TIMP 2 as single proteins in a recombinant vaccinia virus mammalian cell expression system . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Expression of gelatinase A and TIMP 2 mRNAs in desmoplastic fibroblasts in both mammary carcinomas and basal cell carcinomas of the skin . ^^^ AIMS To compare the localisation of mRNAs for the basement membrane degrading enzyme gelatinase A ( 72 kilodalton type 4 collagenase ) and its inhibitor TIMP 2 in carcinomas of the breast and basal cell carcinomas of the skin which have little or no ability to metastasize . ^^^ CONCLUSIONS Maximal expression of gelatinase A and TIMP 2 mRNAs occurs in malignant neoplasms as part of the host response to the presence of established neoplastic cells rather than as an initial response to invasion . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Stability analysis of latent and active 72 kDa type 4 collagenase : the role of tissue inhibitor of metalloproteinases 2 ( TIMP 2 ) . ^^^ We have examined the role of the proteinase inhibitor tissue inhibitor of metalloproteinases 2 ( TIMP 2 ) on two of these processes : the autoactivation and autodegradation of the human 72 kDa type 4 collagenase . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
The 72 kDa type 4 collagenase is secreted as a latent proenzyme , complexed with tissue inhibitor of metalloproteinase 2 ( TIMP 2 ) . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
The mechanism of inhibition of gelatinase A by the TIMPs was investigated by comparing the kinetics of association of TIMP 1 , TIMP 2 , the C terminal deletions , and the mutants of both TIMPs which consisted of the N terminal domain only . ^^^ The full length TIMPs inhibited gelatinase A rapidly with association constants of 3 . 2 10 10 ( 6 ) M 1 s 1 for TIMP 1 and 2 . 1 10 10 ( 7 ) M 1 s 1 for TIMP 2 at 1 = 0 . 2 . ^^^ The C terminal peptide of TIMP 2 is proposed to exist as an exposed `` tail ' ' responsible for binding to progelatinase A and for increasing the rate of inhibition of active gelatinase A through electrostatic interactions with the C terminal domain of the enzyme . ^^^ The C terminal domains of both TIMP 1 and TIMP 2 participate in low affinity interactions with the C terminal domain of gelatinase A which increase the rate of association by a factor of about 100 in both cases . . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
This difference might be a factor in allowing the gelatinase A C terminal domain to bind to natural inhibitor TIMP 2 . . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Quantitative ELISAs comparing the molar ratios of TIMP 1 : MMP 2 and TIMP 2 : MMP 2 further demonstrate that IL 10 ( 10 40 ng ) preferentially activates TIMP 1 secretion to increase the molar ratio of TIMP 1 : MMP 2 in the presence of increasing amounts of ISF ( 0 60 ng ) . ^^^ IL 10 did not elevate TIMP 2 secretion or influence the molar ratio of TIMP 2 : MMP 2 . ( ABSTRACT TRUNCATED AT 250 WORDS ) . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
EXPERIMENTAL DESIGN : To investigate the role of matrix metalloproteinases ( MMPs ) in the loosening we immunolocalized MMP 1 ( tissue collagenase ) , MMP 2 ( gelatinase A ) , MMP 3 ( stromelysin 1 ) , MMP 9 ( gelatinase B ) and their common inhibitors , tissue inhibitors of metalloproteinases ( TIMP 1 and TIMP 2 ) , in the bone prosthesis interface membranes . ^^^ MMP 2 and TIMP 2 were immunolocalized mainly in the fibroblasts . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
MMP 1 ( tissue collagenase ) , MMP 9 ( gelatinase B ) and TIMP 2 were immunolocalized in carcinoma cells and MMP 2 ( gelatinase A ) on tumor cell membranes , whereas no or little immunostaining for MMP 3 ( stromelysin 1 ) and TIMP 1 was seen in carcinoma cells . ^^^ MMP 1 ( tissue collagenase ) , MMP 9 ( gelatinase B ) and TIMP 2 were immunolocalized in carcinoma cells and MMP 2 ( gelatinase A ) on tumor cell membranes , whereas no or little immunostaining for MMP 3 ( stromelysin 1 ) and TIMP 1 was seen in carcinoma cells . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Immunohistochemical studies were performed on frozen tumor samples from 42 patients with invasive bladder cancer treated by cystectomy with monoclonal antibodies against the Mr 72 , 000 gelatinase A ( MMP 2 ) , Mr 92 , 000 gelatinase B ( MMP 9 ) , and TIMP 2 to evaluate their significance in bladder cancer . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
By immunoreactivity analysis using monoclonal antibodies , we showed that the C terminal domain [ R 415 631 ; R is residue ] of progelatinase A [ pro matrix metalloproteinase 2 ( proMMP 2 ) ; EC 3 . 4 . 24 . 24 ] affected the immunoreactivity of a one step sandwich enzyme immunoassay ( sandwich EIA ) for tissue inhibitor of metalloproteinases 2 ( TIMP 2 ) in exactly the same way as does proMMP 2 [ Fujimoto , Zhang , Iwata , Shinya , Okada and Hayakawa ( 1993 ) Clin . ^^^ We further confirmed that TIMP 2 complexed with the N terminal domain of pro MMP 2 had fully inhibitory activity against the collagenolytic activity of MMP 1 . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
In the present paper we provide new evidence , using reverse transcription PCR , that treatment of rat mesangial cells with cytochalasin D enhances the steady state level of mRNA of the membrane type matrix metalloproteinase ( MT MMP ) , as well as of gelatinase A , with no change in the level of tissue inhibitor of metalloproteinases 2 ( TIMP 2 ) mRNA . ^^^ Since the TIMP 2 protein level is reduced in conditioned medium from cytochalasin D treated cells , the results of the present study are consistent with a model in which the action of cytochalasin D is to cause extracellular gelatinase A and TIMP 2 to be sequestered at the plasma membrane , forming a heterotrimeric complex with MT MMP . ^^^ In this manner , TIMP 2 may assume a bifunctional role causing : ( 1 ) inhibition of gelatinase A in the extracellular compartment ; and ( 2 ) guiding gelatinase A to activation through a membrane association with MT MMP . . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
TPA treatment of HT 1080 cells induced the expression of interstitial collagenase ( MMP 1 ) and increased the expression of gelatinase B ( MMP 9 ) , tissue inhibitor of metalloproteinases 1 ( TIMP 1 ) , and MT MMP , a membrane bound activator of progelatinase A ( proMMP 2 ) , while MMP 2 and TIMP 2 expression were decreased . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
However , MMP 2 [ Mr 72 , 000 gelatinase / type 4 collagenase ( gelatinase A ) ] and membrane type MMP were constantly expressed , and the expression of tissue inhibitor of metalloproteinase ( TIMP ) 1 and TIMP 2 also was not changed after 3 and 6 days of treatment with UA . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Expression of MMP 1 appeared more intense than that of TIMP 1 and TIMP 2 in highly inflammatory cases . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
These clinical studies indicate that overexpression of factors associated with the invasion of gastric cancer cells through the basement membrane , including E cadherin , MMP 2 , plasminogen activator inhibitor 1 ( PAI 1 ) , and tissue inhibitor metalloproteinase 2 ( TIMP 2 ) , can be predictive of tumor recurrence and overall survival in patients with this disease . . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
In comparison , TIMP 2 and TIMP 3 are excellent inhibitors , binding more rapidly to the catalytic domain of MT 1 MMP than to the catalytic domain of gelatinase A . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Because gelatinase B expression is linked to invasive potential , we studied the expression of gelatinase B mRNA and protein in vivo , in implanting trophoblast giant cells , and found that it was expressed and activated during colonization of the maternal decidua . mRNAs for several other MMPs ( stromelysin 1 , stromelysin 3 and gelatinase A ) and MMP inhibitors ( TIMP 1 and TIMP 2 ) were expressed in the undifferentiated stroma toward the outside of the decidua , and TIMP 3 mRNA was expressed in primary and some mature decidual cells during their differentiation . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
In the 11 CCs , MMP 1 , MMP 2 , MMP 3 , MMP 9 , TIMP 1 , and TIMP 2 were expressed in tumor cells and / or tumor stroma in 11 ( 100 % ) , 5 ( 45 % ) , 8 ( 73 % ) , 3 ( 27 % ) , 9 ( 82 % ) , and 9 ( 82 % ) , respectively . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
The expression of mRNAs for matrix metalloproteinases ( MMP 1 [ interstitial collagenase ] and MMP 2 ) and their inhibitors ( TIMP 1 and TIMP 2 ) was also studied in the GFs by Northern hybridization . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Therefore , the present study was designed to investigate the molecules responsible for the invasion potential of HCC by focusing on matrix metalloproteinase ( MMP ) in particular , MMP 2 and MMP 9 and the corresponding tissue inhibitor of metalloproteinase ( TIMP 2 and TIMP 1 ) , because these enzymes participate in the degradation of the extracellular matrix including the basement membrane . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Northern blotting demonstrated that MT MMP expression was increased together with that of MMP 2 and its inhibitor gene , TIMP 2 , in embryos depending upon the number of days after gestation , and decreased with maturation after birth . ^^^ In situ hybridization and immunohistochemistry localized MT MMP mRNA and protein in the cells of ossifying tissues where both MMP 2 and TIMP 2 were expressed . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Total RNA was isolated and subjected to Northern blot analysis using cDNA clones for human interstitial collagenase ( MMP 1 ) , 72 kDa type 4 collagenase ( MMP 2 ) and TIMP 2 . ^^^ UVA irradiation resulted in an increase in MMP 1 mRNA up to 2 . 3 fold , but did not stimulate MMP 2 or TIMP 2 mRNA expression . ^^^ In contrast , ROS induced by the xanthine and XOD system resulted in a dose related increase in the level of MMP 2 mRNA up to 2 . 1 fold and a decrease in the level of TIMP 2 mRNA by 49 % in the same fibroblasts . ^^^ Catalase , used as scavenger , essentially prevented the ROS induced alterations in MMP 2 and TIMP 2 mRNA levels . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Purified ESAF is also shown to reactivate the complexes of gelatinase A , collagenase and stromelysin 1 with TIMP 2 . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Oligonucleotide primers for stromelysin 1 , 72 kDa gelatinase , tissue inhibitor of metalloproteinases 1 and 2 ( TIMP 1 and TIMP 2 ) , and GAPDH were synthesized and optimized for use in the reverse transcription polymerase chain reaction ( RT PCR ) . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
The relationship between the serum matrix metalloproteinase 2 ( MMP 2 ) : tissue inhibitor of metalloproteinases 2 ( TIMP 2 ) ratio and disease recurrence was examined in 53 urothelial cancer patients with muscular invasion or with lymph node metastasis who underwent complete resection . ^^^ The mean MMP 2 : TIMP 2 ratio in 31 patients with recurrence was significantly higher than that in 22 patients without recurrence ( P < 0 . 05 ) . ^^^ Disease free survival of patients with high MMP 2 : TIMP 2 ratios was extremely poor compared with that of patients with lower ratios ( P < 0 . 01 ) . ^^^ Cox ' s multivariate analysis suggests that the serum MMP 2 : TIMP 2 ratio would be a new independent prognostic indicator of urothelial cancer recurrence . . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
We examined production and tissue localization of matrix metalloproteinase ( MMP ) 1 ( tissue collagenase ) , MMP 2 ( gelatinase A ) , MMP 3 ( stromelysin 1 ) , MMP 9 ( gelatinase B ) , tissue inhibitors of metalloproteinase ( TIMP ) 1 and TIMP 2 in human breast carcinomas . ^^^ In more than half of the cases , MMP 1 , MMP 2 , MMP 9 , TIMP 1 and TIMP 2 were immunolocalized in carcinoma cells and MMP 2 was on the carcinoma cell membranes as well , whereas MMP 3 was positively stained in less than 15 % of the cases . ^^^ The TIMP 1 levels released into the culture media by carcinoma tissues were significantly lower than those by fibroadenoma tissues , although there were no significant differences in the levels of MMP 1 , MMP 2 , MMP 9 and TIMP 2 . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
The present study was conducted to investigate the potential role of the matrix metalloproteinase 2 ( MMP 2 ) and its specific tissue inhibitors ( TIMP 1 and TIMP 2 ) in the accumulation of matrix components in PKD . ^^^ These findings indicate that tubular MMP 2 activity is reduced in PKD , due to down regulation of MMP 2 , up regulation of TIMP 1 and TIMP 2 , and luminal secretion of the enzyme . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
MMP 2 is inhibited in vivo by tissue inhibitors of metalloproteinases ( TIMPs ) TIMP 1 and , especially , TIMP 2 . ^^^ A synthetic , biotinylated inhibitor specific for activated MMP 2 in solution phase , and immunohistochemistry were used to detect MMP 2 and TIMP 2 expression in cell lines and ovarian tumors and to analyze the surface binding capacity of the inhibitors , which are potential therapeutic agents . ^^^ Characterization of novel monoclonal antibodies to MMP 2 and TIMP 2 is described together with immunocytochemical staining of 83 paraffin embedded ovarian tumors ( 67 malignant , 7 borderline , 9 benign ) and 9 cell lines . ^^^ MMP 2 and TIMP 2 showed heterogenous immunoreactivity , with enhanced staining on high grade tumors , specifically at the invasive front and in vascular invasion . ^^^ This study demonstrates cell surface binding of a synthetic MMP 2 inhibitor and provides new evidence of MMP 2 and TIMP 2 immunoreactivity in ovarian carcinomas and cell lines . . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
The proenzyme forms of MMP 2 ( gelatinase A ) and MMP 9 ( gelatinase B ) are found in complex with tissue inhibitor of metalloproteinases ( designated proMMP 2 / TIMP 2 and proMMP 9 / TIMP 1 , respectively ) . ^^^ In contrast , no significant change in tryptophan accessibility accompanies mercurial treatment of either proMMP 2 or TIMP 2 alone , or mercurial activated MMP 2 mixed with TIMP 2 . ^^^ To determine whether the enhanced fluorescence quenching was unique to the activated proMMP 2 / TIMP 2 complex , similar experiments were performed using MMP 1 , MMP 3 , and MMP 9 / TIMP 1 complex . ^^^ These data , together with our previous biochemical observation that mercurial treatment of proMMP 2 / TIMP 2 exposes the MMP 2 active site without propeptide processing ( Y . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
All conditioned medium of stromal tissues could increase in both MMP 2 , MMP 9 , TIMP 1 , and TIMP 2 activity in SAOV cells . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Dot blot analysis of gelatinase A and TIMP 2 mRNA levels revealed increasing gelatinase A mRNA levels ( HE > E > H > MCF10A ) and reduced TIMP 2 expression in both single and double transformants . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
In order to understand the mechanism for neoplastic cell invasion , we utilized binding studies of TIMP 2 , gelatinase A and the TIMP 2 / gelatinase A complex to neoplastic cells and correlated these results with their capacity to invade a matrix substrate in a modified Boyden chamber assay . ^^^ Binding studies were performed on malignant human breast cancer cells and fibrosarcoma cells with rTIMP 2 , rGelatinase A , and TIMP 2 / gelatinase A complex . ^^^ Competition studies of the binding characteristics of these proteins indicated that gelatinase A and gelatinase A / TIMP 2 complex bound to the surface of cells via TIMP 2 . ^^^ A working hypothesis and model for the role of gelatinase A / TIMP 2 complex in cellular invasion is presented . . ^^^ TIMP 2 mediates cell surface binding of MMP 2 . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
In normal lung , MMP 2 , MMP 9 , TIMP 1 , and TIMP 2 were localized in ciliated cells , endothelial cells , pneumocytes , macrophages , and smooth muscle cells ; fibroblasts showed a strong reaction only for MMP 2 . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
The conditioned medium from the cystic tubules contained higher than normal levels of MMP 9 , MMP 2 , and MMP 3 as well as TIMP 1 and TIMP 2 . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
OBJECTIVE : To investigate the correlation between serum and synovial fluid ( SF ) concentrations of stromelysin 1 ( MMP 3 ) , gelatinases ( MMP 2 and MMP 9 ) , tissue inhibitors of metalloproteinases ( TIMP 1 and TIMP 2 ) in patients with rheumatoid arthritis ( RA ) and to assess the role of these proteins in cartilage destruction and their clinical value as indicators of disease activity . ^^^ In addition , there was significant negative correlation between SF concentrations of TIMP 2 and MMP 2 , 3 , and 9 . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
This investigation was based on flow cytometry analysis after propidium iodide and acridine orange staining , histology of tumour parenchyma and RT PCR analysis for the type 4 collagenases MMP 9 and MMP 2 and their respective inhibitors TIMP 1 and TIMP 2 mRNAs . ^^^ Na [ trans RuCl 4 ( DMSO ) Im ] also influences a proteolytic system which has the potential of degrading the basement membrane and has been related to metastatic aggressiveness : it markedly reduces , in a dose dependent manner , MMP 2 / TIMP 2 balance , but not that of MMP 9 / TIMP 1 . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
To evaluate the expression of TIMP 1 , TIMP 2 , MMP 2 , and MMP 9 in human colorectal cancer , surgical specimens of primary colorectal cancer ( 66 cases ) and liver metastases ( 10 cases ) were examined by Northern and dot blot hybridization . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Antibodies to MMP 9 , MMP 2 , TIMP 1 , and TIMP 2 were applied by immunohistochemical methods . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
METHODS : By immunocytochemical staining , hyperplastic polyps , tubular adenomas , tubovillous adenomas , villous adenomas to adenocarcinomas were systematically examined for the presence of MMP 2 ( gelatinase A ) and MMP 9 ( gelatinase B ) and tissue inhibitor of MMP ( TIMP ) 1 and TIMP 2 , respectively . ^^^ RESULTS : MMP 2 and MMP 9 , and TIMP 1 and TIMP 2 were immunolocalized in scattered stromal cells , whereas epithelial cells of normal mucosa and hyperplastic polyps were weakly stained . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
However , treatment of P 9 or P 10 cells with graded doses of TGF beta 1 increased expression of both collagen 4 and gelatinase A mRNA and did not alter the ratio of signal intensity for TIMP 2 mRNA species . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
We have examined the influence of different cytokines ( interleukin [ IL ] 10 , IL 4 , IL 6 , IL 2 , and interferon gamma ) on TIMP 2 , MMP 2 , and MMP 9 protein and mRNA expression in human papillomavirus ( HPV ) 18 immortalized human prostate cell lines derived from the primary cultures . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
A second unglycosylated inhibitor , TIMP 2 , which is less abundant , has the interesting property of binding to the proform of gelatinase A and is involved in controlling its activation . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Immunohistochemical staining was performed for collagenase ( MMP 1 ) , gelatinase A ( MMP 2 ) , stromelysin ( MMP 3 ) , TIMP 1 , and TIMP 2 . ^^^ MMP 2 was increased in the diseased aortas , and TIMP 2 was abundant in normal , AOD , and AAA samples . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Zymography and Western blot analysis showed that : ( 1 ) human peritoneal mesothelial cells produced and excreted MMP 2 and MMP 9 and their inhibitors TIMP 1 and TIMP 2 ; ( 2 ) hyperosmolality drastically reduced the expression of MMP 9 irrespective of the osmolyte used in a time and concentration dependent manner ; ( 3 ) TGF beta 1 unexpectedly increased MMP 9 activity and protein in exponentially growing cells and could restore MMP 9 activity suppressed by hyperosmolality in confluent cultures . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
This study was designed to investigate possible involvement of type 4 collagenolytic matrix metalloproteinases ( MMPs ; 72 kDa type 4 collagenase [ MMP 2 ] , 92 kDa type 4 collagenase [ MMP 9 ] ) , and the respective specific tissue inhibitors of these MMPs ( TIMP 2 and TIMP 1 ) in the development of adult respiratory distress syndrome ( ARDS ) . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Northern blot analysis and in situ hybridization showed that , in both pure and co cultures , HSCs , but not hepatocytes , expressed MMP 2 , TIMP 2 , and MT MMP 1 mRNA . ^^^ These data show that the co expression of TIMP 2 , MMP 2 , and MT MMP 1 by HSCs does not lead to secretion of the activated form of MMP 2 . ^^^ Hepatocytes , which do not express MMP 2 , TIMP 2 , or MT MMP 1 , induce MMP 2 activation through a plasma membrane dependent mechanism ( s ) , thus suggesting that cell cell interactions are involved in this process in vivo . . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
By immunocytochemically staining pancreatic tissue , which contained exocrine duct adenocarcinoma , normal islets were incidentally found positive for gelatinase A ( MMP 2 ) and gelatinase B ( MMP 9 ) , and for tissue inhibitor of metalloproteinase 1 and 2 ( TIMP 1 and TIMP 2 ) . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
We have studied the expression of gelatinase A , gelatinase B , interstitial collagenase , tissue inhibitor of metalloproteinase ( TIMP ) 1 and TIMP 2 in reactive lymphoid cells , as well as in a series of cell lines derived from neoplasms of B and T cell lineage . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
MMP 2 , TIMP 1 and TIMP 2 were immunolocalized in more than 70 % of the cases of both BCL and CHS , but the positive scores of these were not statistically different between the two groups . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Effect of glucose and heparin on mesangial alpha 1 ( 4 ) COLL and MMP 2 / TIMP 2 mRNA expression . ^^^ In human and murine mesangial cell cultures , RT PCR was able to detect mRNA expression for a number of molecules involved in the mesangial extracellular matrix turnover : type 4 collagen [ alpha 1 ( 4 ) COLL ] , MMP 1 , MMP 2 , MMP 3 , MMP 9 and MMP 10 , and the tissue inhibitors TIMP 1 and TIMP 2 . ^^^ The expression of mRNA for alpha 1 ( 4 ) COLL and MMP 2 / TIMP 2 balance was studied in human cells in the presence of high glucose and heparin . mRNAs for all the studied molecules were expressed at different levels . ^^^ Interestingly , a shift in the balance of alpha 1 ( 4 ) COLL , MMP 2 and TIMP 2 was observed in high glucose , which was partially reversed by heparin supplementation . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
We measured serum MMP 1 , MMP 2 , TIMP 1 and TIMP 2 levels using the respective one step sandwich enzyme immunoassays in 98 patients with chronic hepatitis C treated with interferon beta to examine their clinical significance for assessment of liver histology and to determine whether they can be useful as predictors of the interferon response . ^^^ In logistic multivariate regression analysis , the ratios of serum MMP 2 to TIMP 1 level ( p=0 . 0001 ) , HCV genome subtype ( p=0 . 005 ) and serum TIMP 2 level ( p=0 . 03 ) were the independent predictors for sustained response , while serum MMP 2 level ( p=0 . 0006 ) was the only predictor for no response . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
In situ detection of matrix metalloproteinase 2 ( MMP 2 ) and the metalloproteinase inhibitor TIMP 2 transcripts in human primary hepatocellular carcinoma and in liver metastasis . ^^^ We have investigated the cellular sources of MMP 2 and TIMP 2 in primary and secondary human liver cancers . ^^^ The cellular sources of MMP 2 and TIMP 2 were further characterized using an anti alpha smooth muscle actin antibody on contiguous sections . ^^^ RESULTS : In hepatocellular carcinoma and liver metastases , in situ hybridization showed that MMP 2 and TIMP 2 mRNA were expressed by anti alpha smooth muscle actin positive cells at the invasive front . ^^^ Intratumor microvessels showed a strong labeling for MMP 2 but weak for TIMP 2 mRNA . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
We report that cytokines secreted by infected glial cells are responsible for the increased expression of MMP 3 , MMP 9 and TIMP 3 , while MMP 2 , TIMP 1 and TIMP 2 remained stable . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Gelatinase A mRNA transcripts were strongly expressed in the perichondrium of Meckel ' s cartilage and mesenchymal areas of embryonic day 13 15 mandibles , whereas gelatinase B , collagenase 3 , TIMP 1 and TIMP 2 mRNA were found primarily in the ossifying areas of the mandibles . ^^^ The skeletal muscle of the tongue expressed stromelysin 3 , TIMP 2 and TIMP 3 mRNA while stromelysin 3 , TIMP 2 and gelatinase A were seen in the overlying connective tissue layer . ^^^ Gelatinase A , gelatinase B , stromelysin 1 , urokinase , TIMP 1 and TIMP 2 mRNA and protein activities were also detected in cultured mandibular explants . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
TIMP 2 RNA distribution largely matched with previously reported patterns of matrix metalloproteinase 2 ( 72 kd gelatinase ) expression , suggesting generation of a TIMP 2 / matrix metalloproteinase 2 complex ( large inhibitor of metalloproteinases ) . ^^^ Addition of transforming growth factor beta 1 enhanced TIMP 1 and matrix metalloproteinase 2 RNA levels in vitro , whereas TIMP 2 specific signals were reduced , likely to result in a stoichiometric excess of matrix metalloproteinase 2 over TIMP 2 . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
To inhibit cancer invasiveness , the tissue inhibitor of the MMP 2 ( TIMP 2 ) , which is a natural inhibitor of MMPs , was overexpressed in MDA 231 cells . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
In the present study , the authors evaluated immunohistochemically the expression of gelatinase A ; tissue inhibitor of metalloproteinases 2 ( TIMP 2 ) , an inhibitor of gelatinase A ; matrilysin ; and trypsin ( ogen ) in 67 lung tumors from a variety of histological types including 17 squamous cell carcinomas , 16 adenocarcinomas , 15 small cell carcinomas , and 12 carcinoids . ^^^ Interestingly , normal bronchial , bronchiolar , and alveolar epithelial cells expressed gelatinase A , TIMP 2 , matrilysin , and trypsin ( ogen ) at varying frequencies and intensities . ^^^ Bronchial smooth muscle cells and cartilage cells expressed gelatinase A alone , whereas endothelial cells , fibroblasts , and macrophages expressed gelatinase A and TIMP 2 . ^^^ The present results , taken together with those of previous studies , suggest that gelatinase A is associated with malignant behavior of all the types of lung tumors , whereas its activity may be controlled by the endogenous inhibitor TIMP 2 . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Molecular regulation of cellular invasion role of gelatinase A and TIMP 2 . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Both mRNA and immunodetectable protein of MT MMP 1 are increased with monensin treatment while message for the protein inhibitor of gelatinase A , TIMP 2 , is unchanged . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
In light and / or electron microscopic immunohistochemistry , the MMP 1 , 2 , 9 and TIMP 2 antibodies reacted with MF in both types of corneal wounds . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
The expression of MMP 2 , MMP 9 , TIMP 1 , TIMP 2 , and the urokinase receptor were examined in fetal and normal prostate tissues , benign prostatic hyperplasia and prostate cancer ( n = 117 ) . ^^^ In situ hybridization with digoxigenin labeled oligonucleotide probes demonstrated that TIMP 1 and TIMP 2 were expressed at elevated levels in the stroma of Gleason sum 5 tissues , whereas MMP 2 and MMP 9 were expressed at relatively low levels . ^^^ In higher Gleason sum tissues ( GS 8 10 ) , TIMP 1 and TIMP 2 were not expressed , whereas MMP 2 and MMP 9 were intensely expressed . ^^^ The data suggest that TIMP 1 ( and TIMP 2 ) and MMP 2 ( and MMP 9 ) are independent predictors of outcome . . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
The effect of treatment with paclitaxel was also determined separately on certain invasion associated events , such as the secretion of 72 kDa type 4 collagenase ( gelatinase A / MMP 2 ) , the expression of the tissue inhibitor of metalloproteinase 2 ( TIMP 2 ) , cell attachment and migration . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
We present evidence for the first time of a specific , high affinity interaction between TIMP 4 and the C domain of human gelatinase A and show that TIMP 4 binds both progelatinase A and the C domain in a similar manner to that of TIMP 2 . ^^^ Competition between TIMP 2 and TIMP 4 for a common or overlapping binding sites on the gelatinase A C domain may occur ; alternatively TIMP 2 may prevent the binding of TIMP 4 by steric hindrance or induction of a conformational change in the C domain . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
This study determined whether transcription of matrix metalloproteinase ( MMP ) 9 and MMP 2 , or tissue inhibitors of metalloproteinase ( TIMP ) 1 and TIMP 2 , or human papillomavirus ( HPV ) open reading frames ( ORFs ) is associated with invasive behavior in cervical carcinoma cells . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
MMP 2 ( 72 kD type 4 collagenase ) , TIMP 1 and TIMP 2 were expressed in all five cell lines . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
The role of the C terminus , which has closer homology with TIMP 2 than TIMP 1 , was also investigated : a C truncated mutant , similarly cloned and expressed in E . coli , shows complete lack of inhibitory activity on MMP 9 , still retaining some on MMP 2 . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Steady levels of 72 kD type 4 collagenase ( MMP 2 ) , stromelysin ( MMP 3 ) , and tissue inhibitor of metalloproteinase 2 ( TIMP 2 ) mRNA were significantly increased 2 days after induction of pancreatitis . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
The influence of dibutyril cyclic AMP ( dbcAMP ) on the gene expression of matrix metalloproteinases ( MMP ) 1 , MMP 2 , MMP 3 , tissue inhibitors of metalloproteinases ( TIMP ) 1 and TIMP 2 was investigated in human skin fibroblasts by northern analyses . ^^^ Procollagen alpha 1 ( 1 ) , MMP 2 and TIMP 2 mRNA levels were unaltered by this reagent . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
This study proposes to quantify relative mRNA levels for interstitial collagenase ( MMP 1 ) , 72 kd type 4 collagenase ( MMP 2 ) , 92 kd type 4 collagenase ( MMP 9 ) , TIMP 1 , and TIMP 2 in normal aorta ( NA ) and AAA to provide insight as to the relative importance of each in aneurysm formation . ^^^ RESULTS : Significant increases in MMP mRNA expression in AAA over NA were observed for MMP 1 ( 3 . 64 versus 0 . 3 , p = 0 . 007 ) , MMP 9 ( 78 . 03 versus 3 . 35 , p = 0 . 003 ) , TIMP 1 ( 835 . 32 versus 477 . 2 , p = 0 . 027 ) , and TIMP 2 ( 18 . 09 versus 4 . 14 , p = 0 . 003 ) . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Since the liver is a main target for metastases from gastrointestinal adenocarcinoma , we have investigated MMP 2 and TIMP 2 expression by RT PCR , in situ hybridization and zymography in the liver of patients with gastrointestinal adenocarcinomas and no detectable hepatic metastasis ( n = 12 ) , in tumoral and nontumoral liver from patients with hepatic metastasis ( n = 9 ) and in control liver ( n = 4 ) . ^^^ MMP 2 and TIMP 2 mRNA levels were increased in liver from patients with gastrointestinal adenocarcinomas and no detectable metastasis , compared with those of either control liver ( 5 fold and 3 . 2 fold , respectively ) or nontumoral areas of liver from patients with metastasis ( 7 . 8 fold and 3 fold , respectively ) . ^^^ MMP 2 and TIMP 2 transcripts were located in mesenchymal cells of portal tracts and sinusoids . ^^^ In liver from patients with hepatic metastasis , the tumoral / nontumoral ratios for MMP 2 and TIMP 2 mRNA were 6 . 2 + / 4 and 1 . 5 + / 0 . 4 , respectively . ^^^ In the matching nontumoral areas the signals for MMP 2 and TIMP 2 mRNA were restricted to mesenchymal cells in portal tracts and sinusoidal cells . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
We investigated the temporal and spatial expression of mRNAs for four matrix metalloproteinases ( MMPs ; MMP 2 [ gelatinase A ] , MMP 3 [ stromelysin 1 ] , MMPs ; MMP 2 [ gelatinase B ] , and MMP 13 [ collagenase 3 ] ) and tissue inhibitors of metalloproteinases ( TIMPs ; TIMP 1 , TIMP 2 , and TIMP 3 ) in the mouse uterus from days 1 to 8 of pregnancy . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
RESULTS : The reactivity of Langerhans ' cells was moderate to intense for MMP 2 , weaker for MMP 9 , and faint for TIMP 1 and TIMP 2 . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
To evaluate the role of matrix metalloproteinases ( MMPs ) and their specific tissue inhibitors ( TIMPs ) in the pathogenesis of the structural damage and cystic lesions found in pulmonary lymphangioleiomyomatosis ( LAM ) , immunohistochemical studies were made of the localization of MMP 1 , MMP 2 , MMP 3 , MMP 9 , TIMP 1 , TIMP 2 , HMB 45 , and type 4 collagen in sections of lung biopsy specimens from four patients with this disorder . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
The aim of this study was to investigate whether immunohistochemical staining patterns of tissue inhibitor of metalloproteinases TIMP 2 and matrix metalloproteinases MMP 2 and MMP 9 can be predictors of tumour stage and survival time in colorectal cancer . ^^^ Three mouse monoclonal antibodies T 2 101 against TIMP 2 , CA 4001 against MMP 2 and GE 213 against MMP 9 were used . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Expression of tissue inhibitor of metalloproteinase 2 ( TIMP 2 ) , a specific inhibitor of MMP 2 , was similarly inhibited by cytokines in these cell clones . ^^^ Thus , the net balance estimated by MMP 2 / TIMP 2 suggested enhanced proteolysis in cytokine treated NS SV AC and , to a much lesser extent , in NS SV DC . ^^^ As such , it seems likely that there is a relationship between the activation of MMP 2 and the activity of NF kappa B , and that the NF kappa B / I kappa B alpha complex is not a signal mediator involved in cytokine induced suppression of TIMP 2 . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
MTMMP was expressed concurrently with MMP 2 in 64 per cent of tumours , but concurrent MMP 2 and TIMP 2 expression occurred in only half . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Kinetic analysis of the binding of human matrix metalloproteinase 2 and 9 to tissue inhibitor of metalloproteinase ( TIMP ) 1 and TIMP 2 . ^^^ SPR analysis shows biphasic kinetics with high ( nM ) and low ( microM ) affinity binding sites of TIMP 2 and TIMP 1 for MMP 2 ( 72 and 62 kDa species ) and MMP 9 ( 92 and 82 kDa species ) , respectively . ^^^ In contrast , binding data of TIMP 2 to an MMP 2 45 kDa active form lacking the C terminal domain and to an MMP 2 C terminal domain ( CTD ) fragment displays monophasic kinetics with Kd values of 315 and 60 nM , respectively . ^^^ Also , binding of TIMP 2 to pro MMP 2 is stronger at both the high and low affinity sites than the corresponding binding of TIMP 2 to the MMP 2 62 kDa form demonstrating the importance of the N terminal prodomain . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Cultured fibroblasts from patients with systemic sclerosis ( SSc ) and normal individuals were examined for gene expression of types 1 and 3 collagen , decorin , matrix metalloproteinases ( MMP ) MMP 1 , MMP 2 , and MMP 3 , tissue inhibitors of metalloproteinases ( TIMP ) TIMP 1 and TIMP 2 , urokinase and tissue type plasminogen activators ( u PA and t PA ) . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Northern blotting and zymography of primary tumor crude extracts revealed that treatment with either razoxane or dacarbazine for one generation approximately doubled the expression of MMP 2 and MMP 9 , while lacking any effect on that of 1 . 0 and of 3 . 5 kb TIMP 2 . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
The expression of Gelatinase A ( MMP 2 ) and its specific tissue inhibitor ( TIMP 2 ) , a degradative system whose balance is involved in matrix invasion and metastasis , was investigated in SK N BE cells cultured with and without CNTF or NGF . ^^^ In a time course experiment in the presence of 25 ng / ml of CNTF , the MMP 2 mRNA expression showed no significant modulation , while TIMP 2 mRNA up regulated to > 2 fold after 48 h and then fell dramatically . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Antibodies used in this study were monoclonal antibodies to MMP 1 , MMP 2 , MMP 7 , MMP 8 , MMP 9 , TIMP 1 , TIMP 2 and polyclonal antibody to MMP 3 . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Enhanced invasion was not associated with significant changes in the expression of uPA or its membrane receptor UPAR ; plasminogen activator inhibitors PAI 1 and PAI 2 ; metalloproteinases MMP 1 , MMP 2 , MMP 3 , MMP 9 and membrane type MMP 1 ; or tissue inhibitors of metalloproteinases TIMP 1 and TIMP 2 . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
To determine the effect of mineral fibers and cigarette smoke on remodeling of lung tissues , we examined matrix metalloproteinase 1 ( MMP 1 ) , MMP 2 , tissue inhibitors of metalloproteinase 1 ( TIMP 1 ) , TIMP 2 , and types 1 and 4 collagen mRNA levels from rat lungs exposed to mineral fibers and / or cigarette smoke in vivo . ^^^ Exposure to cigarette smoke induced increases in MMP 1 and TIMP 1 mRNA levels and decreased TIMP 2 and type 1 collagen mRNA levels in lung . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Using Western blotting , we examined MMP 2 and MMP 9 and tissue inhibitor of metalloproteinases ( TIMP ) 1 and TIMP 2 expression . ^^^ Thapsigargin at 10 nmol / L did not significantly increase cell death or MMP 2 , MMP 9 , TIMP 1 , and TIMP 2 expression . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Stable transfection of U251 . 3 glioma cells with cDNA encoding MT MMP 1 resulted in increased cell surface expression of MT MMP 1 and TIMP 2 , constitutive activation of MMP 2 proenzyme and increased collagen degradation . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Our data thus suggest that interstitial collagenase , gelatinase A , and TIMP 1 play a role in homeostasis of the pleural space in vivo as constitutively expressed proteins , whereas gelatinase B and TIMP 2 expression are induced in specific disease states . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
An immunohistochemical study on cryosections was performed using antibodies to 72 kDa gelatinase , 92 kDa gelatinase , TIMP 1 , TIMP 2 , laminin , collagen types 1 , 3 , 4 , 7 . mRNA expression for gelatinases and their inhibitors were also analyzed by reverse transcriptase polymerase chain reaction ( RT PCR ) . ^^^ In order to evaluate the role of gelatinases in remodelling psoriatic skin in this study we examined the production of the 72 kDa ( gelatinase A ) , 92 kDa collagenase ( gelatinase B ) and their tissue inhibitors TIMP 2 and TIMP 1 . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Modulation of MMP 2 activation by exogenous TIMP 2 reverted the rate of Matrigel invasion by cell transfectants to control levels . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
We report a dose dependent increase in secretion of both MMP 2 and tissue inhibitor of metalloproteinases 2 ( TIMP 2 ) in response to vitronectin . ^^^ MMP 2 and MMP 2 / TIMP 2 interaction with the plasma membrane of melanoma cells resulted in enhanced catalytic activity against 14C labeled gelatin , suggesting that membrane association may function in posttranslational regulation of MMP 2 activity . ^^^ Binding of proMMP 2 and proMMP 2 / TIMP 2 to melanoma cells was not inhibited by RGDS , and melanoma cell adhesion to vitronectin was unaffected by pro or active MMP 2 , indicating that MMP 2 did not interact with the murine vitronectin receptor . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
The same region of gelatinase A was required for binding of latent and active enzyme to TIMP 2 , but residues 418 474 were not important . ^^^ Together , the data suggest that TIMP 2 forms a receptor with MT 1 MMP that regulates the concentration and efficient generation of functionally active gelatinase A . . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
We therefore examined the expression of mRNA and protein for MMPs ( interstitial collagenase , MMP 1 ; gelatinases , MMP 2 and MMP 9 ; and stromelysin , MMP 3 ) and TIMPs ( TIMP 1 and TIMP 2 ) in leiomyoma and matched unaffected myometrium from GnRHa ( lupron ) treated and untreated patients . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
The present study examined changes in the expression of membrane type metalloproteinase ( MT MMP 1 ) , a putative regulator of MMP 2 , in the tissue localization of MMP 2 , and in the expression of activated and latent forms of MMP 2 and the tissue inhibitor of metalloproteinases , TIMP 2 , in rat carotid arteries subjected to balloon catheter injury . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
The expression of genes for MMP 2 and TIMP 2 in human diabetic nephropathy was investigated . ^^^ Contrary to TIMP 2 which was expressed both in tubulo interstitium and glomeruli in almost all renal biopsies , MMP 2 gene down regulation was observed in glomeruli from all NIDDM patients , irrespective of the albumin excretion rate , and of renal histology . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Serum levels of tissue inhibitor of metalloproteinases 2 ( TIMP 2 ) and of precursor form of matrix metalloproteinase 2 ( proMMP 2 ) were determined in patients with chronic hepatitis and hepatocellular carcinoma by a one step sandwich enzyme immunoassay . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Rous sarcoma virus transformed chicken embryo fibroblasts ( RSVCEF ) , when compared to normal CEF , produce elevated levels of matrix metalloproteinase 2 ( MMP 2 ) that exists in a form free of complexed tissue inhibitor of metalloproteinase 2 ( TIMP 2 ) . ^^^ In order to ascertain whether the increased levels of TIMP free MMP 2 in RSVCEF cultures are due to diminished expression of TIMP 2 or alterations in TIMP 2 that diminish its MMP 2 binding ability , it was necessary to clone , characterize , and express chicken TIMP 2 cDNA . ^^^ Addition of recombinantly expressed TIMP 2 to RSVCEF cultures causes a disappearance of TIMP free ( TF ) proMMP 2 with a corresponding increase in the TIMP complexed ( TC ) proMMP 2 levels , demonstrating that TF proMMP 2 is capable of converting to TC pro MMP 2 when free TIMP 2 is available . ^^^ Surprisingly , RSVCEF cultures manifest a TIMP 2 population that is not complexed to MMP 2 , despite the coexistence of TIMP free proMMP 2 . ^^^ Thus transformed cells can alter the TIMP 2 / MMP 2 balance by transcriptional and post translational modifications , yielding a population of inhibitor free , proteolytically active MMP2 . . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
The matrix metalloproteinase ( MMP ) inhibitor TIMP 2 has a high specificity for gelatinase A / MMP 2 . ^^^ An imbalance between gelatinase A and TIMP 2 in favor of enzymatic activity is linked to the degradation of the extracellular matrix ( ECM ) associated with several physiologic and pathologic events , including angiogenesis , invasion and metastasis . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Analysis of the expression of MTI MMP , TIMP 2 and alpha ( 5 ) integrin , 3 proteins shown to play a role in cell surface association of MMP 2 , revealed enhanced levels of these proteins in confluent MDA MB 231 cells . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
The expression of MMP 2 and TIMP 2 in patients with colorectal adenocarcinoma invaded to the submucosal and proper muscle layer . ^^^ The expression of MMP 2 and TIMP 2 was examined immunohistochemically in a total of 87 colorectal cancer patients . ^^^ These results suggest that the expression of MMP 2 and TIMP 2 is induced in the sm cancer stage and is closely related to interactions in the cancer interstitial region for invasion and metastasis in patients with colorectal adenocarcinoma . . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
The data suggest that VEGF may modulate endothelial cell derived MMP activity by : ( 1 ) increasing the abundance of gelatinase A ; ( 2 ) disinhibiting gelatinase A by decreasing the abundance of TIMP 2 ; and ( 3 ) disinhibiting preexisting collagenase by reducing levels of TIMP 1 . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Serum levels of matrix metalloproteinase 2 ( MMP 2 ) and tissue inhibitor of metalloproteinases 2 ( TIMP 2 ) were evaluated as prognostic indicators of the recurrence of urothelial cancer . ^^^ Sera were obtained from 127 healthy control subjects and 97 urothelial cancer patients who underwent complete resection and were measured for MMP 2 and TIMP 2 using a one step enzyme immunoassay . ^^^ The relationship between the serum MMP 2 / TIMP 2 ratio and the recurrence of urothelial cancer was examined . ^^^ The mean serum MMP 2 / TIMP 2 ratio in the 31 advanced urothelial cancer patients with recurrence was significantly higher than that in the 22 patients without recurrence ( P = 0 . 0029 ) and in the 44 superficial bladder cancer patients ( P < 0 . 0001 ) . ^^^ The 1 and 3 year disease free survival rates in the patients with high MMP 2 / TIMP 2 ratios ( 50 % and 12 % ) were significantly poorer than those of the patients with normal ratios ( 82 % and 56 % ) ( P = 0 . 0152 ) . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
In contrast , MMP 2 and TIMP 2 levels were not changed by PDGF and / or mechanical strain . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
To evaluate the roles of MMP 2 , its inhibitor ( tissue inhibitor of metalloproteinase 2 [ TIMP 2 ] ) , and its activator ( membrane type matrix metalloproteinase 1 [ MT 1 MMP ] ) in tumor invasion or as a prognostic factor in patients with bladder carcinoma , the authors investigated the expression of MMP 2 , TIMP 2 , and MT 1 MMP in patients with bladder carcinoma . ^^^ Expression of MMP 2 , TIMP 2 , MT 1 MMP , and glyceraldehyde 3 phosphate dehydrogenase was examined by reverse transcriptase polymerase chain reaction analysis . ^^^ Correlations between the levels of MMP 2 , TIMP 2 , and MT 1 MMP expression and histologic findings or patient outcome were evaluated . ^^^ RESULTS : Expression of MMP 2 and TIMP 2 was significantly higher in muscle invasive pT 2 < or = bladder tumors than in pT1a tumors ( MMP 2 : P < 0 . 0005 ; TIMP 2 : P < 0 . 005 ) . ^^^ Moreover , high levels of MMP 2 and TIMP 2 , as well as MT 1 MMP expression , all were strongly associated with decreased survival ( MMP 2 : P < 0 . 0001 ; TIMP 2 : P < 0 . 0001 ; and MT 1 MMP : P < 0 . 005 ) . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Localization of TIMP 1 , TIMP 2 , TIMP 3 , gelatinase A and gelatinase B in pathological human corneas . ^^^ PURPOSE : Determine the tissue distribution patterns for tissue inhibitors of metalloproteinases ( TIMP 1 , TIMP 2 , TIMP 3 ) , gelatinase A and gelatinase B in normal and pathologic corneas . ^^^ METHODS : Corneas were examined by immunohistochemistry , using antibodies to TIMP 1 , TIMP 2 , TIMP 3 , gelatinase A or gelatinase B . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Zymograms were used to evaluate stromelysin , gelatinase A and B activity levels , while immunoblots of Western transfers were used to evaluate stromelysin , collagenase , TIMP 1 and TIMP 2 protein levels . ^^^ RESULTS : A phorbol mitogen ( TPA ) , and TNF alpha and beta , interleukin 1 alpha and PDGF BB stimulate gelatinase B , stromelysin , interstitial collagenase and TIMP 1 expression , while having negligible effects on gelatinase A expression ; TIMP 2 levels are reduced by TNF but not affected by the other treatments . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
RNA levels of membrane type 1 matrix metalloproteinase ( MT 1 MMP ) , MMP 2 , and MMP 9 and of their inhibitors TIMP 2 and TIMP 3 in normal thyrocytes and thyroid carcinoma cell lines . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
In contrast , matrilysin did not proteolytically cleave gelatinase A TIMP 2 complex , but led to a transient increase in gelatinolytic activity of the proenzyme . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Enzyme linked immunosandwich assays ( ELISAs ) revealed that IL 10 ( 15 ng / ml for 36 h ) treatment of the PC 3 ML cells down regulated MMP 2 and MMP 9 while up regulating TIMP 1 ( not TIMP 2 ) expression . ^^^ In comparison to IL 10 , IL 4 failed to influence metastasis or the expression of TIMP 1 , TIMP 2 , MMP 2 and MMP 9 by PC 3 ML cells . ^^^ We suggest that IL 10 regulated increases in the molar ratio of TIMP 1 / MMP 9 and TIMP 2 / MMP 2 might inhibit processes critical to the establishment of bone marrow metastasis . . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Antibodies to MMP 2 and TIMP 2 reacted with fibroblast cytoplasm in a granular pattern . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
The ratios of TIMP 1 to TIMP 2 and MMP 9 to MMP 2 were significantly higher in malignant tumor tissues than in normal ovaries ( P < 0 . 001 ) . ^^^ TIMP activity consisting of TIMP 1 and TIMP 2 correlated significantly to MMP activity of MMP 2 and MMP 9 ( r = 0 . 67 , P < 0 . 005 ) . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
In this study we examined the production of gelatinases A and B ( MMP 2 and MMP 9 ) , and their natural inhibitors TIMP 1 and TIMP 2 in cell lines derived from different histologic types of lung cancer . ^^^ Immunocytochemistry and Western blot analysis were performed to corroborate the presence of immunoreactive MMP 2 , MMP 9 , TIMP 1 and TIMP 2 proteins . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Relative LV myocardial abundance of interstitial collagenase ( MMP 1 ) , stromelysin ( MMP 3 ) , 72 kD gelatinase ( MMP 2 ) , 92 kD gelatinase ( MMP 9 ) , TIMP 1 , and TIMP 2 were measured with quantitative immunoblotting . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
PROBLEM : The finding of MMP 2 ( which degrades type 4 collagen ) and TIMP 2 ( the tissue inhibitor of MMP ) in fetal membranes suggests the possibility of membrane self destruction as an etiology of premature rupture of fetal membranes . ^^^ The finding of MT 1 MMP in a tissue already known to produce MMP 2 and TIMP 2 documents the full system for activation and inhibition of this gelatinase . ^^^ During infection , an imbalance in the expression of MT 1 MMP , MMP 2 and TIMP 2 may constitute an endogenous pathway of membrane degradation . . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
The lack of MMP 2 activation in cell lines was attributed to the absence of expression of a membrane type MMP ( MT 1 MMP ) , which activates proMMP 2 , and to the abundant expression of TIMPs , particularly TIMP 2 . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Alendronate failed to inhibit secretion of tissue inhibitor of metalloproteinase 2 ( TIMP 2 ; the inhibitor of MMP 2 ) and the decrease in collagen 1 solubilization observed may occur , in part , from changes in the molar stoichiometry of TIMP 2 to MMP 2 . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
In this study , the occurrence of MMP 1 , MMP 2 , MMP 3 and MMP 9 and the two tissue inhibitors of metalloproteinases , TIMP 1 and TIMP 2 , was monitored in the cerebrospinal fluid ( CSF ) from patients with viral meningitis . ^^^ Of the other MMPs and TIMPs assayed , MMP 2 and TIMP 2 were constitutively expressed and not upregulated in viral meningitis . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Activation induced by GRGDSP and cytochalasin B was cell mediated , inhibited by EDTA , tissue inhibitor of metalloproteinase 2 ( TIMP 2 ) and carboxyl terminal MMP 2 consistent with a role for membrane type ( MT ) MMP but did not involve urokinase , plasmin or thrombin activity . ^^^ Activation induced by GRGDSP and cytochalasin B , but not cell rounding , was inhibited by herbimycin A , cycloheximide and actinomycin D , suggesting a role for tyrosine kinases , protein and RNA synthesis , but was not associated with changes in mRNA for MT MMP 1 , MMP 1 , MMP 2 , TIMP 1 or TIMP 2 . ^^^ GRGDSP and cytochalasin B enhanced levels of membrane associated pro and active form MMP 1 and MMP 2 but not MT MMP 1 , stimulated cell surface MMP 1 staining and induced that of MT MMP 1 , MMP 2 and TIMP 2 . ^^^ This was consistent with the possible relocation of constitutive MT MMP 1 to the cell surface as a prerequisite for subsequent cell surface MMP 2 / TIMP 2 / MT MMP 1 complex formation and to the potential induction of conditions favourable for reciprocal cell surface MMP 1 / MMP 2 activation . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Immunohistochemical analysis for the detection of fibronectin , collagen 1 , 3 , and 4 , smooth muscle actin , MMP 1 ( interstitial collagenase ) , 2 ( gelatinase A ) , and 9 ( gelatinase B ) , and TIMP 2 , and in situ hybridization for the detection of MMP 9 mRNA were performed . ^^^ MMP 1 , MMP 2 , MMP 9 , and TIMP 2 were detected in the regenerated epithelial cells covering intra alveolar fibrosis . ^^^ MMP 1 , MMP 2 , MMP 9 , and TIMP 2 were detected in the regenerated epithelial cells covering intra alveolar fibrosis . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Immunohistochemical analysis revealed increased expression of MT 1 MMP , MMP 2 , and TIMP 2 in fibroblasts , synovial lining like cells , and endothelial cells , as well as , to some extent , in monocyte / macrophage like cells in both tissues from the bone implant interface and reactive cellular tissues from regenerating capsules in loose hip joints , when compared with control fibrous tissues between bone and implants retrieved from unloosened hip joints . ^^^ In loose hip joints , reverse transcription PCR analysis showed the presence of MT 1 MMP , MMP 2 , and TIMP 2 mRNA in both the bone implant interface and regenerating capsular tissues . ^^^ Increased protein levels of MMP 2 and TIMP 2 were also demonstrated by an ELISA , and those of MT 1 MMP were shown by immunoblot analysis . ^^^ Collectively , these data suggest that the MT 1 MMP / MMP 2 / TIMP 2 system participates in the extracellular matrix degradation and periprosthetic connective tissue remodeling in loose hip joints , and may thus contribute to the periprosthetic weakening , loosening , and osteolysis that can occur around implants . . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
The activity of MMP 2 is inhibited by a `` soluble ' ' tissue inhibitor of MMP 2 , TIMP 2 . ^^^ METHODS : MT 1 MMP was cloned from embryonic mouse kidney cDNA library , and its spatio temporal distribution during development in the context of MMP 2 and tissue inhibitor of metalloproteinase 2 ( TIMP 2 ) was studied . ^^^ TIMP 2 expression was also developmentally regulated , but lagged behind MT 1 MMP and MMP 2 . ^^^ Similar spatio temporal expressions of MMP 2 and TIMP 2 proteins were observed . ^^^ CONCLUSIONS : mRNAs of MT MMP 1 and MMP 2 are expressed in the respective epithelial and mesenchymal compartments , while their proteins are co expressed in the epithelia suggest that MT 1 MMP and MMP 2 , in conjunction with TIMP 2 , may be involved in paracrine / juxtacrine epithelial : mesenchymal interactions during metanephrogenesis . . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
MMP 1 , MMP 2 , MMP 9 , TIMP 1 , TIMP 2 , and TIMP 3 were investigated by zymography , reverse zymography , Northern blotting , and in situ hybridization . ^^^ There was no change in the expression of MMP 1 , TIMP 1 , and TIMP 2 throughout the luteal phase or after luteal rescue . ^^^ Messenger ribonucleic acids ( mRNAs ) for MMP 1 , MMP 2 , and TIMP 2 were localized to the connective tissue stroma and the thecal lutein cells of the corpus luteum . ^^^ MMP 1 , MMP 2 , MMP 9 , TIMP 1 , TIMP 2 , and TIMP 3 were investigated by zymography , reverse zymography , Northern blotting , and in situ hybridization . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
The specific expression patterns of MMP 1 and TIMP 2 were detected and regulated by RA in almost all cell lines , whereas expression of MMP 2 and TIMP 1 was not influenced by RA treatment . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Kinetic analysis of the mechanism of interaction of full length TIMP 2 and gelatinase A : evidence for the existence of a low affinity intermediate . ^^^ We have undertaken a detailed analysis of the mechanism of inhibition of matrix metalloproteinase 2 ( gelatinase A ) by tissue inhibitor of metalloproteinase 2 ( TIMP 2 ) . ^^^ Quenched fluorescent substrates have been used to analyze the rate of inhibition of gelatinase A by TIMP 2 over a wide range of TIMP 2 concentrations . ^^^ By studying the dissociation of 125I labeled TIMP 2 from a gelatinase A TIMP 2 complex using ligand exchange experiments , we obtained a rate constant for the dissociation of the final stable complex of 2 10 10 ( ) 8 s 1 . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
We used northern blot analysis of RNA from fresh frozen tumour specimens to study the patterns of expression of genes for proteolytic enzymes ( cysteine proteinase cathepsin L and aspartyl proteinase cathepsin D ; matrix metalloproteinases MMP 9 and MMP 2 ) , tissue inhibitors of metalloproteinases TIMP 1 and TIMP 2 , and TP 53 . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
The degradation of tissue inhibitor of metalloproteinase ( TIMP ) free matrix metalloproteinase ( MMP ) 2 to proteolytically inactive fragments by plasmin was inhibited in equimolar mixtures of purified TIMP 2 and TIMP free MMP 2 and was not observed in purified MMP 2 TIMP 2 complexes . ^^^ Divalent cation chelators EDTA and sodium Alendronate did not inhibit plasmin degradation of TIMP free MMP 2 but reversed the ability of TIMP 2 to protect MMP 2 from degradation by plasmin . ^^^ Our data confirm a role for plasmin in the clearance of TIMP free MMP 2 , identify a pivotal role for TIMP 2 in regulating MMP 2 longevity in plasmin containing environments , and highlight a novel therapeutic use for chelators of divalent cations , including the bisphosphonate Alendronate , in the reversal of TIMP 2 protection of MMP 2 from degradation by plasmin . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
In order to investigate the correlation between expressions of TIMP 2 , MMPs and clinical outcome , immunohistochemical staining of MMP 2 , MMP 9 , and TIMP 2 were performed on paraffin embedded tissue sections of 15 early gastric cancers ( EGC ) and 15 advanced gastric carcinomas ( AGC ) without nodal metastasis and 15 AGC with nodal metastasis ( AGCn+ ) . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Role of tissue inhibitor of metalloproteinases 2 ( TIMP 2 ) in regulation of pro gelatinase A activation catalyzed by membrane type matrix metalloproteinase 1 ( MT 1 MMP ) in human cancer cells . ^^^ To clarify the regulatory mechanism of pro gelatinase A ( proGelA ) activation at a cellular level , expression of gelatinase A ( GelA ) , three MT MMPs , and TIMP 2 was examined with 11 human cancer cell lines cultured in the presence and absence of stimulants . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Immunoblot analysis with specific antibodies against MMP 2 and MMP 9 and their tissue inhibitors ( TIMP 1 , TIMP 2 ) disclosed that , independent of the phase of the patients ' HIV infection , their salivary samples contained higher amounts of MMP 2 and MMP 9 immunoreactivities in pro and active forms and the TIMP 1 and TIMP 2 inhibitors than did the control samples . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Competition based reverse transcription polymerase chain reaction revealed that nondiabetic and diabetic HRECs expressed constitutively mRNA for MMP 2 , MMP 9 , tissue inhibitor of metalloproteinase ( TIMP ) 1 , TIMP 2 , and fibronectin . ^^^ After exposure to 5 or 30 mmol / l glucose , no changes were detected in mRNA levels in MMP 2 or MMP 9 , their inhibitors TIMP 1 and TIMP 2 , or fibronectin in either nondiabetic or diabetic HREC cultures . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Relaxin produced a dose dependent induction of collagenase 1 ( MMP 1 ) and stromelysin 1 ( MMP 3 ) , but minimal modulation of TIMP 1 and TIMP 2 expression in the fibrocartilaginous cells . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Both the activation of pro MT 1 MMP and the membrane binding of the tissue inhibitor of metalloproteinases type 2 ( TIMP 2 ) and gelatinase A , and subsequent activation of gelatinase A , were inhibited by calcium ionophores . ^^^ Low expression levels of TIMP 2 and rapid synthesis of MT 1 MMP were found to be critical for gelatinase A activation . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
We then assessed the effect of fibronectin on the components of the putative MT 1 MMP / TIMP 2 ' receptor ' complex implicated in MMP 2 activation . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
However , the kinetics and mechanisms of their inhibitory effects were different , because TIMP 2 and the COOH terminal domain of MMP 2 preferentially inhibited the MT 1 MMP dependent and autocatalytic steps of MMP 2 activation , respectively . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
The present study examines the effect of transforming growth factor beta 1 ( TGF beta 1 ) and interleukin 1beta ( IL 1beta ) on the regulation of gelatinase A , gelatinase B , tissue inhibitor of metalloproteinase 1 ( TIMP 1 ) and TIMP 2 in human glomerular epithelial cells ( GEC ) . ^^^ Of particular interest was the observation of the differential regulation of gelatinase A and its specific inhibitor TIMP 2 ( which binds to the latent form of this enzyme ) in response to TGF beta 1 . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Additionally , transcripts for the recently described membrane associated matrix metalloproteinase , MT 1 MMP ( MMP 14 ) , which can act as an activating receptor for MMP 2 / TIMP 2 complexes ( Strongin et al . [ 1995 ] J . ^^^ TIMP 2 and 3 follow a pattern of expression similar to the MMP 2 protein . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
TIMP 2 bound to the membrane in a HXM insensitive manner , comprising about 40 50 % of TIMP 2 on the membrane , is the inhibitor of the cell surface activated MMP 2 . ^^^ The inhibition of the 65 kDa MMP 2 by TIMP 2 is initiated by the interaction of their C terminal domains . ^^^ TIMP 2 complex is released from the membrane , and the activity of MMP 2 is blocked by TIMP 2 . ^^^ In the presence of collagen types 1 , 2 , 3 , 5 , or gelatin , the rate of inhibition of the 65 kDa MMP 2 by the membrane bound TIMP 2 decreased considerably . ^^^ These results suggest that the pericellular activity of MMP 2 is tightly regulated by membrane bound TIMP 2 and surrounding extracellular matrix components . . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Gelatinase A ( MMP 2 ) was maximally increased at 5 days , and TIMP 2 was highest at 5 days . ^^^ Gelatinase A ( MMP 2 ) was maximally increased at 5 days , and TIMP 2 was highest at 5 days . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
The aim of this study was to investigate the extracellular degrading proteolytic cascade proteins referred to as matrix metalloproteinase 1 ( MMP 1 ) , MMP 2 , MMP 9 , membrane type matrix metalloproteinase 1 ( MT 1 MMP ) , tissue inhibitors of matrix metalloproteinase 1 ( TIMP 1 ) , TIMP 2 , neutrophil elastase , and alpha 1 antitrypsin in human pulmonary emphysema . ^^^ Localization of MMP 1 , MMP 2 , MMP 8 , MMP 9 , MT 1 MMP , TIMP 1 , and TIMP 2 was verified by immunohistochemical analysis . ^^^ The presence of MMP 1 , MMP 2 , MMP 9 , TIMP 1 , and TIMP 2 was confirmed at mRNA level by reverse transcription PCR analysis and enzyme immunoassay ( EIA ) . ^^^ The data suggest that the MT 1 MMP / MMP 2 / TIMP 2 system plays a significant role in the MMP mediated extracellular matrix degradation and tissue remodeling of emphysematous lungs , and thus may contribute to the weakening of lung parenchyma and lead to the formation of emphysema . . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
In situ hybridization anti sense and sense riboprobes were generated using DNA complementary to human collagenase ( MMP 1 ) , 72 kDa gelatinase ( MMP 2 ) , stromelysin ( MMP 3 ) , 92 kDa gelatinase ( MMP 9 ) , TIMP 1 , TIMP 2 , and TIMP 3 . ^^^ MMP 2 , TIMP 1 , and TIMP 2 mRNA had a similar overall distribution that was relatively uniform within the vascularized membrane stroma . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Immunohistochemical staining and in situ hybrydization showed that MMP 2 , MMP 9 , TIMP 1 and TIMP 2 were expressed by both tumor cells and stromal cells , with stronger staining at the site of tumor invasion . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
The purpose of this study was to correlate the presence of MMP 2 , MMP 9 and their inhibitors with the tissue inhibitors of metalloproteinases TIMP 1 and TIMP 2 RNA using reverse transcriptase PCR technique with tumor stage in 17 samples of renal cell carcinoma . ^^^ The ratio of tissues expressing MMP 2 and MMP 9 to those expressing TIMP 1 and TIMP 2 was defined to be 1 in normal kidney tissue . ^^^ These data suggest that the balance of MMP 2 and MMP 9 to TIMP 1 and TIMP 2 expression is an essential factor in the aggressiveness of renal cell carcinoma . . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Eighteen cervical adenocarcinomas and 5 controls were immunohistochemically analyzed for the expression of MMP 2 , MMP 3 , MMP 9 , and their inhibitors , TIMP 1 and TIMP 2 , in tumor cells and peritumoral stromal cells . ^^^ These cells were also studied for the presence of MMP 2 , MMP 9 , and TIMP 2 mRNA by in situ hybridization ( ISH ) . ^^^ MMP 2 and 9 were expressed immunohistochemically in tumor cells in 17 of 18 tumors , MMP 3 in 5 , TIMP 1 in 3 , and TIMP 2 in 1 . ^^^ The normal endocervical epithelium was uniformly negative for MMP 2 , MMP 3 , MMP 9 , and TIMP 2 , and variably expressed TIMP 1 . ^^^ Intense signals for MMP 2 , MMP 9 , and TIMP 2 mRNA were less frequently detected by ISH in tumor cells and peritumoral stromal cells and were absent in normal endocervical epithelium . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Proteolytic activity was determined with substrate gel zymography , and enzyme content was determined with Western immunoblotting using monoclonal antibodies directed against MMP 2 , MMP 3 , MMP 9 , TIMP 1 , TIMP 2 , and alpha 2 macroglobulin . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
The MT MMP / TIMP 2 / MMP 2 system thus provides localized expression of proteolysis of the extracellular matrix required for cell migration . . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Several MMPs of different substrate specificities were found to be expressed in rat pancreatic islets , either shortly after islet isolation and in all conditions tested ( MMP 9 , TIMP 1 ) or after a lag time ( MMP 2 , MMP 3 , MMP 14 , TIMP 2 ) . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
METHODS : The presence of MMP 1 , MMP 2 , MMP 3 , MMP 9 , TIMP 1 , and TIMP 2 was identified in a group of gastric cancers ( n=74 ) by immunohistochemistry using monoclonal antibodies . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Expression of four members of the matrix metalloproteinase ( MMP ) family ( MMP 2 / gelatinase A , MMP 3 , MMP 9 / gelatinase B , and MMP 13 ) and three tissue inhibitors of metalloproteinases 1 , 2 , and 3 ( TIMP 1 , TIMP 2 , and TIMP 3 ) were evaluated by Northern blot analysis of RNA from liver tissue isolated at 0 , 2 , 5 , 10 , 20 , and 30 days after either a BDL or sham operation . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Although the precise mechanism of this activation remains elusive , levels of the membrane type 1 MMP ( MT 1 MMP ) at the cell surface and of the tissue inhibitor of MMP 2 ( TIMP 2 ) appear to be two important determinants . ^^^ We did , however , detect decreased levels of TIMP 2 protein in the media of cells incubated with peptide 1 . 1 or recombinant SPARC ; thus , the induction of MMP 2 activation by SPARC might be due in part to a diminution of TIMP 2 protein . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
METHODS AND RESULTS : To test the hypothesis that changes in the activity of the matrix metalloproteinases ( MMPs ) and their tissue inhibitors ( TIMPs ) are responsible for the damage to connective tissue in these lesions , histochemical studies of the immunoreactivity ( IR ) for MMPs and their tissue TIMPs ( MMP 1 , MMP 2 , MMP 3 , MMP 9 , TIMP 1 , and TIMP 2 ) were made in TAAs ( n = 7 ) and aortic valves ( n = 5 ) from 7 patients with Marfan ' s syndrome . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
BACKGROUND : Matrix metalloprotease 2 ( MMP 2 ) is secreted in a latent inactive form ( pro MMP 2 ) that is activated on the cell surface by a membrane type 1 MMP ( MT 1 MMP ) in the presence of the tissue inhibitor of MMP ( TIMP 2 ) . ^^^ In spite of evidence for the synthesis of MT 1 MMP shown by immunoblotting , immunocytochemistry and RT PCR , and of TIMP 2 , MMP 2 was found exclusively in a latent form in human mesangial cells ( HMC ) serum free culture medium . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Gelatinase A ( MMP 2 ) , gelatinase B ( MMP 9 ) , TIMP 1 , and TIMP 2 were present in EOSC conditioned medium , and TIMP 3 in ECM of EOSC . ^^^ Gelatinase A ( MMP 2 ) , gelatinase B ( MMP 9 ) , TIMP 1 , and TIMP 2 were present in EOSC conditioned medium , and TIMP 3 in ECM of EOSC . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Regulation of matrix metalloproteinase 2 ( gelatinase A , MMP 2 ) , membrane type matrix metalloproteinase 1 ( MT 1 MMP ) and tissue inhibitor of metalloproteinases 2 ( TIMP 2 ) expression by elastin derived peptides in human HT 1080 fibrosarcoma cell line . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
METHODS : Thirty nine surgically obtained specimens of the hypopharynx were immunohistochemically stained with antibodies against MMP 1 , MMP 2 , MMP 3 , MMP 9 and their inhibitors , TIMP 1 and TIMP 2 . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Expression levels of MMP 2 , MMP 9 , tissue inhibitor of metalloproteinase ( TIMP ) 1 and TIMP 2 were measured in 24 tumor samples by immunohistochemistry . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
In contrast , HPCA 10aHPV 18 cells secreted high levels of MMP 2 and MMP 9 ( > 40 pg / microg protein ) but low levels of TIMP 1 and TIMP 2 ( < 5 pg / microg protein ) . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
METHODS AND RESULTS : Because oxidized low density lipoprotein ( OxLDL ) modulates gene expression , we investigated the effect of these particles on the levels of MMP 1 , MMP 3 , MMP 9 , TIMP 1 , and TIMP 2 in the culture media of human monocyte derived macrophages . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Biochemical analyses and immunoassays were used to measure the concentrations of MMP 1 , MMP 3 , tissue inhibitor of metalloproteinases 1 ( TIMP 1 ) , TIMP 2 , the disaccharide of hyaluronic acid , the proteoglycan glycosaminoglycan disaccharides of chondroitin 4 sulfate ( delta di CS 4 ) and chondroitin 6 sulfate ( delta di CS 6 ) , the 846 epitope on chondroitin sulfate of cartilage proteoglycan aggrecan ( putative biosynthetic marker ) , the keratan sulfate ( KS ) epitope of aggrecan ( putative degradation marker ) , and the C propeptide of cartilage type 2 procollagen ( CPII ) ( biosynthetic marker ) . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Our aim was to investigate the collagenolytic potential and localization of matrix metalloproteinase 2 ( MMP 2 ) in relation to its regulatory proteins membrane type MT 1 MMP and tissue inhibitor of metalloproteinases 2 ( TIMP 2 ) in rheumatoid arthritis ( RA ) . ^^^ In synovial tissue , MT 1 MMP , TIMP 2 and MMP 2 were found in synovial lining in fibroblast and macrophage like cells , in stromal cells and in vascular endothelium . ^^^ MT 1 MMP , TIMP 2 and MMP 2 were strongly expressed in the pannocytes of the invasive pannus at the interface , but staining was weak and / or there were few positive cells both `` above ' ' and `` below ' ' the soft to hard tissue ( cartilage and / or bone ) interface . ^^^ Rheumatoid synovial tissue extract contained proteolytically active 62 / 59 kDa MMP 2 and 43 kDa MT 1 MMP , but no free TIMP 2 . ^^^ These results indicate that components of the ternary MT 1 MMP / TIMP 2 / MMP 2 complex are coexpressed in the normal synovial lining and in its pathological extension on the hyaline articular cartilage . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
To investigate the roles of gelatinase A , its activator MT 1 MMP , and the MMP inhibitors TIMP 1 and TIMP 2 in aseptic loosening of polyethylene prostheses , immunohistochemistry ( IHC ) and in situ hybridization ( ISH ) were performed on periprosthetic pseudosynovial interface tissues . ^^^ While TIMP 1 was expressed essentially in hyperplastic pseudosynoviocytes as assessed by IHC and ISH , TIMP 2 , MT 1 MMP , and gelatinase A were colocalized in phagocytic cells . ^^^ These data support the concept of progelatinase A activation involving a trimolecular complex ( MT 1 MMP TIMP 2 gelatinase A ) mechanism . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Identification of the tissue inhibitor of metalloproteinases 2 ( TIMP 2 ) binding site on the hemopexin carboxyl domain of human gelatinase A by site directed mutagenesis . ^^^ We have mutated the unique cationic clusters found in hemopexin modules 3 and 4 of the carboxyl domain ( C domain ) of human gelatinase A to determine their role in binding TIMP 2 . ^^^ Thus , these analyses indicate that the TIMP 2 binding surface lies at the junction of hemopexin modules 3 and 4 on the peripheral rim of the gelatinase A C domain . ^^^ C domain complex would be needed for the bound TIMP 2 to inhibit in cis the gelatinase A active site . . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Light microscopic immunohistochemistry for MMP 1 ( interstitial collagenase ) , MMP 2 ( gelatinase A ) , MMP 9 ( gelatinase B ) and TIMP 2 was performed . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
No effect was observed on the expression of MMP 2 and of the MMP inhibitors TIMP 1 and TIMP 2 . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
We examined 23 epiretinal membranes and 15 subretinal membranes of PVR for deposition of interstitial collagenase ( MMP 1 ) , stromelysin 1 ( MMP 3 ) , gelatinase A ( MMP 2 ) , gelatinase B ( MMP 9 ) and two tissue inhibitors of metalloproteinases ( TIMP 1 and TIMP 2 ) by immunohistochemical methods . ^^^ We observed that a large proportion of epiretinal and subretinal membranes stained for MMP 1 and MMP 2 , whilst MMP 3 , MMP 9 , TIMP 1 and TIMP 2 were only observed in a small proportion of specimens . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Analyses of glomerular histological and immunopathological lesions in association with the detection of mRNA expression of collagen 4 , TGF beta , matrix protein turnover related enzymes , matrix metalloproteinase 2 ( MMP 2 ) , tissue inhibitor of metalloproteinase 2 ( TIMP 2 ) and platelet derived growth factor ( PDGF ) were also performed in young ( 10 week old ) and elderly ( 60 week old ) RF / J mice with age matched BALB / C mice as the controls . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Northern blot analysis of mRNA encoding MMP 2 , MT 1 MMP , the physiological activator of MMP 2 , and TIMP 2 , the natural inhibitor of MMP 2 , revealed that OA did not alter the steady state levels of MMP 2 , MT 1 MMP and TIMP 2 mRNA . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
We analyzed the expression of all known MMPs with established elastolytic activity and TIMPs in human AAA and control tissue . mRNA coding for MMP 9 , MMP 2 , human macrophage metalloelastase , MMP 7 , TIMP 1 , and TIMP 2 were amplified by reverse transcriptase PCR in control and AAA tissue . ^^^ A Northern blot assay was used to measure the levels of mRNA coding for MMP 2 , MMP 9 , TIMP 1 , and TIMP 2 . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
In the present study , we investigated the expression of several matrix metalloproteinases ( MMPs ) and tissue inhibitors of matrix metalloproteinases ( TIMPs ) , including MMP 1 , MMP 2 , MMP 3 , MMP 7 , MMP 9 , MT 1 MMP , TIMP 1 and TIMP 2 , in these tumors . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Plasma levels of matrix metalloproteinase 2 ( MMP 2 ) and tissue inhibitors of metalloproteinases 1 and 2 ( TIMP 1 and TIMP 2 ) as noninvasive markers of liver disease in chronic hepatitis C : comparison using ROC analysis . ^^^ We determined if plasma levels of MMP 2 , TIMP 1 and TIMP 2 are related to liver histology in patients with chronic hepatitis C . ^^^ Plasma MMP 2 , TIMP 1 and TIMP 2 levels were measured in 43 patients with chronic hepatitis C . ^^^ Plasma levels of MMP 2 , TIMP 1 and TIMP 2 and serum ALT were correlated with liver biopsy score and specificity and sensitivity of the assays in detecting advanced liver disease were calculated from ROC analysis . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
To clarify the process and mechanisms of the development and progression of peripheral lung adenocarcinoma , we investigated the relationships among the patterns of basement membrane ( BM ) , stromal fibrosis , and the expressions of gelatinase A and tissue inhibitor of metalloproteinases 2 ( TIMP 2 ) in 33 lesions of atypical alveolar cell hyperplasia ( AAH ) and 48 lesions of lung adenocarcinoma , including 24 lesions of bronchioloalveolar carcinoma ( BAC ) . ^^^ The expression of gelatinase A and TIMP 2 was associated with central scar formation as well as with destruction of the BM components . ^^^ Both the neoplastic and stromal cells expressed gelatinase A and TIMP 2 and probably play a role in tumor cell invasion . . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
METHODS : Messenger RNA expression of gelatinase A , gelatinase B , matrilysin and tissue inhibitor of metalloproteinases ( TIMP ) 1 and TIMP 2 were analyzed in 30 patients with hepatocellular carcinoma by semiquantitative reverse transcription polymerase chain reaction ( RT PCR ) . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Gelatinase A and stromelysin 3 were found to be prominently expressed in decidual tissue ; shortly after midpregnancy , the decidual expression patterns of gelatinase A and stromelysin 3 became overlapping with each other , as well as with the expression domain of TIMP 2 . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
We found that treatment of TIMP 2 with cyanate ion led to loss of inhibitory activity toward matrilysin or gelatinase A . ^^^ The reactive site modified TIMP 2 also prevented an accumulation of active gelatinase A on the cell surface . ^^^ We speculate that occupation of the hemopexin like domain of gelatinase A by the reactive site modified TIMP 2 makes it unable for gelatinase A to be retained on the cell surface , thus preventing the autocatalytic conversion of the intermediate form of gelatinase A to its mature form . . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
In this study we examined the effects of FGF 1 and FGF 1 plus heparin on type 1 collagen , collagen binding stress protein HSP 47 , interstitial collagenase ( matrix metalloproteinase [ MMP ] 1 ) , gelatinase A , and tissue inhibitor of metalloproteinase ( TIMP ) 1 and TIMP 2 expression by normal human lung fibroblasts . ^^^ Neither FGF 1 nor FGF 1 plus heparin affected the expression of TIMP 1 , TIMP 2 , and gelatinase A . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
During early tooth development , gelatinase A and Timp 2 were widely expressed in the branchial arch , while gelatinase B and Timp 1 and Timp 3 expression showed clear association with epithelial morphogenesis and was restricted to the mesenchyme at the tip of the growing tooth bud . ^^^ Gelatinase A and Timp 1 showed transient expression in secretory odontoblasts at the time of basement membrane degradation , while Timp 2 expression continued throughout the dental papilla . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
We compared the expression of matrix metalloproteinases ( MMP 2 and MMP 9 ) and tissue inhibitors of metalloproteinases ( TIMP 1 and TIMP 2 ) in bone marrow acute myelogenous leukaemia ( AML ) blasts and leukaemic cell lines ( HEL , HL 60 , K 562 and KG 1 ) with their expression in normal bone marrow cells . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
METHODS : We examined the localization of MMP 1 , MMP 2 , MMP 9 and TIMP 2 in normal human and rabbit stomachs by light and electron microscopic immunohistochemistry and Western blotting , and the enzymatic activities of MMP 2 and MMP 9 by gelatin zymography . ^^^ Electron microscopic immunohistochemistry of parietal cells showed immunolabeling of MMP 2 and TIMP 2 in the cisternae of the rough endoplasmic reticulum , and that of MMP 1 and MMP 9 in tubular structures in their cytoplasm . ^^^ CONCLUSION : Normal rabbit and human stomachs contain MMP 1 , MMP 2 , MMP 9 , and TIMP 2 and these are mainly localized in , and synthesized by parietal cells . . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Reverse transcriptase PCR analysis revealed the presence of TIMP 2 mRNA in the majority of MMP 2 releasing BM MNCs . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Using a cell transfection system that altered the ratio of MMP 2 to TIMP 2 we have demonstrated significant variation in the adhesive phenotype of tumor cells . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
In addition to collagenases 1 and 3 that have been implicated in invasive growth behaviour of various malignant tumours , we analysed the levels of 72 kDa gelatinase and its activators MT 1 MMP and TIMP 2 in cultured melanoma cells . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
In conclusion , NO produced by C 3 L5 cells promoted tumor cell invasiveness by altering the balance between MMP 2 and its inhibitors TIMP 2 and 3 . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
On the transcriptional level , RT PCR was used to evaluate the expression of membrane type ( MT ) 1 MMP , MMp 2 , MMP 9 and the inhibitors TIMP 1 and TIMP 2 in tumour tissue and normal kidney tissue . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
The present study evaluated the role of matrix metalloproteinase 2 ( MMP 2 ) , tissue inhibitor of metalloproteinases 2 ( TIMP 2 ) , and membrane type metalloproteinase ( MT 1 MMP ) in cervical neoplasia . ^^^ MMP 2 and TIMP 2 expression correlates with poor prognosis in cervical carcinoma a clinicopathologic study using immunohistochemistry and mRNA in situ hybridization . ^^^ METHODS : We have analyzed 49 uterine cervical squamous cell carcinomas , 10 cases of high grade cervical intraepithelial neoplasia ( CIN 2 3 ) , and 10 control cervices for the presence of MMP 2 , TIMP 2 , and MT 1 MMP using in situ hybridization . ^^^ RESULTS : MMP 2 , TIMP 2 , and MT 1 MMP mRNA were localized to both stromal and tumor cells . ^^^ The presence of both MMP 2 and TIMP 2 mRNA in tumor cells correlated with advanced stage ( P = 0 . 003 for MMP 2 , P = 0 . 002 for TIMP 2 ) and with poor survival ( P = 0 . 003 for MMP 2 , P = 0 . 002 for TIMP 2 ) in univariate analysis . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
In the present experiment , the expression of matrix metalloproteinase ( MMP ) 2 and MMP 9 , key proteins in the MMP family , and the tissue inhibitors of metalloproteinase ( TIMP ) 1 and TIMP 2 , antagonistic proteins against MMP 2 and MMP 9 , respectively , were investigated by Northern blot analysis in rat transplantable osteosarcomas with high and low metastatic potencies . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Levels of mRNAs for interstitial collagenase , gelatinase A , matrilysin , TIMP 1 and TIMP 2 increased ( p < 0 . 05 ) within 12 h of hCG , while gelatinase B mRNA increased later , by 36 h after hCG . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
First , two PGE 2 receptor subtypes , EP 3 and EP 4 , are aberrantly expressed in the uterine stroma in Hoxa 10 ( / ) mice , while expression of several other genes in the stroma ( TIMP 2 , MMP 2 , ER , and PR ) and epithelium ( LIF , HB EGF , Ar , and COX 1 ) are unaffected before implantation . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
By preparing biopsies from healthy skin and liposclerotic lesions , MMP 1 , MMP 2 , MMP 9 , TIMP 1 , and TIMP 2 were analyzed by using reverse transcriptase polymerase chain reaction , western blot , zymography , hydrolysis of [ 3H ] labeled collagens , and immunohistochemistry . ^^^ Our investigations provide evidence that mRNA and protein expression of MMP 1 , MMP 2 , and TIMP 1 were significantly increased in lipodermatosclerosis , whereas the total amount of MMP 9 and TIMP 2 mRNA and protein was not altered . ^^^ Furthermore , MMP 2 was imbalanced by locally reduced expression of TIMP 2 in the basement membrane zone of lesional skin . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
In order to assess the significance of changes in metalloproteinase activity in pancreatic carcinogenesis , the expression of matrix metalloproteinases 2 and 9 ( MMP 2 and MMP 9 , respectively ) , tissue inhibitor of metalloproteinase 1 ( TIMP 1 ) and TIMP 2 , and membrane type 1 MMP ( MT 1 MMP ) and MT 2 MMP in ductal lesions in a rapid production model for pancreatic duct carcinomas ( PCs ) in hamsters initiated with N nitrosobis ( 2 oxopropyl ) amine ( BOP ) and in subcutaneous transplantable tumors of hamster pancreatic duct carcinoma ( HPDs ) was investigated . ^^^ Northern analysis revealed MMP 2 , MMP 9 , TIMP 2 and MT 1 MMP mRNAs to be overexpressed in PCs . ^^^ These results indicate that overexpression of MMP 2 , TIMP 2 and MT 1 MMP , and cell surface activation of proMMP 2 by MT 1 MMP , are involved in the development of PCs , and that MMP 2 expression at the protein level appears in the early phase of pancreatic duct carcinogenesis . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
By contrast , the level of TIMP 1 mRNAs was not altered significantly and that of TIMP 2 was reduced mildly by the HGF / SF treatment , suggesting that HGF / SF may eventually modulate a balance between MMP 2 and TIMPs in favor of the proteinase activity in the glioma cell microenvironment . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
TIMP 3 , TIMP 1 and gelatinase A mRNAs were elevated in most human OA synovia over controls , while TIMP 2 expression was similar . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
We have demonstrated , using human airway smooth muscle cells in culture , that 1 ) ASM releases gelatinase A constitutively and when stimulated with PMA and tumor necrosis factor alpha releases gelatinase B , and the release of gelatinase B is protein kinase C dependent because it is blocked by H 7 and staurosporin ; 2 ) treatment of ASM cells with PMA or concanavalin A failed to activate progelatinase A despite these agents increasing cell expression of MT 1 MMP ; and 3 ) the inability of ASM cell membranes to activate progelatinase A is most likely secondary to the high levels of TIMP 2 on the cell membrane . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
To determine the ability of radiation to modulate mesangial cell expression of various molecules involved in promoting extracellular matrix ( ECM ) accumulation [ fibronectin , plasminogen activator inhibitor 1 ( Pai 1 ) , and tissue inhibitor of metalloproteinase 2 ( Timp 2 ) ] and degradation ( Tgfb , plasminogen activators u PA or t PA , matrix metalloproteinases Mmp 2 and Mmp 9 ) , primary cultures of rat mesangial cells ( passage number 6 11 ) were placed in serum free medium 24 h prior to irradiation with single doses of 0 . 5 20 Gy ( 137 ) Cs gamma rays . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Lack of TIMP 2 near the migrating epithelial wound edges might contribute to uncontrolled activity of MMP 2 in chronic ulcers . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Although lamellipodia exhibit similar structure and mobility to invadopodia , lamellipodia , by virtue of their location at the cellular periphery , are readily accessible to the soluble tissue inhibitor of matrix metalloproteinase 2 ( TIMP 2 ) and blood borne inhibitors . ^^^ Specialized surface protrusions of invasive cells , invadopodia and lamellipodia , have differential MT 1 MMP , MMP 2 , and TIMP 2 localization . ^^^ We show here that TIMP 2 co localizes with MT 1 MMP and MMP 2 at lamellipodia but not with that of invadopodia . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Expression and localization of TIMP 1 , TIMP 2 , MMP 13 , MMP 2 , and MMP 9 in early and advanced experimental lung silicosis . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Identification of the TIMP 2 binding site on the gelatinase A hemopexin C domain by site directed mutagenesis and the yeast two hybrid system . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
The expression of MMP 2 and TIMP 2 in patients with primary colorectal adenocarcinoma : correlation with liver metastasis . ^^^ The expression of MMP 2 and TIMP 2 was examined immunohistochemically in a total of 36 colorectal cancer patients with synchronous liver metastasis at the time of surgery , and the serum MMP 2 and TIMP 2 levels were also measured in a total of 58 colorectal cancer patients with / without liver metastasis following primary colorectal surgical resection for serological comparison . ^^^ Although MMP 2 exhibited a significant expression immunohistochemically in the primary colorectal cancer ( p < 0 . 05 ) and TIMP 2 in the synchronous liver metastasis ( p < 0 . 01 ) , there was no relationship between the absence / presence of liver metastasis and serum MMP 2 and TIMP 2 levels , respectively . ^^^ These results suggest that it can be considered difficult to use serum MMP 2 and TIMP 2 levels to predict the status of liver metastasis following primary resection in patients with colorectal adenocarcinoma . . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Expression of metalloproteinases ( MMP 1 , MMP 2 , and MMP 9 ) and their inhibitors ( TIMP 1 and TIMP 2 ) in schistosomal portal fibrosis . ^^^ Focal extracellular matrix degradation morphologically identified in human portal pipestem fibrosis due to Schistosoma mansoni did not express immunohistochemical reactivity for metalloproteinases ( MMP 1 , MMP 2 , and MMP 9 ) and their inhibitors ( TIMP 1 and TIMP 2 ) . ^^^ However , when active schistosomal periovular granulomas were present , a strong reactivity for MMP 1 , MMP 2 , TIMP 1 , and TIMP 2 was observed . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Northern blot hybridization showed that messenger RNA for several MMPs and TIMPs , including gelatinase A , MT 1 MMP , stromelysin 3 , MMP 19 , TIMP 1 , TIMP 2 , and TIMP 3 , were present at detectable levels in the mouse ovary . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
It was the aim of this study to determine whether the expression of MMP 1 , MMP 2 , and TIMP 2 correlates with survival in patients with surgically treated endometrial cancer . ^^^ RESULTS : MMP 1 , MMP 2 , and TIMP 2 were detected by immunohistochemistry in 95 % ( 98 / 103 ) , 87 % ( 89 / 103 ) , and 80 % ( 82 / 103 ) of the tumour samples , respectively . ^^^ Correlation coefficients for MMP 1 / MMP 2 , MMP 1 / TIMP 2 , MMP 2 / TIMP 2 were 0 . 28 ( p = 0 . 004 ) , 0 . 05 ( p = 0 . 6 ) , and 0 . 03 ( p = 0 . 73 ) , respectively . ^^^ In the univariate analysis , the expression of MMP 1 ( log rank test , p = ns ) , MMP 2 ( log rank test , p = ns ) , and TIMP 2 ( log rank test , p = ns ) were not associated with overall survival . ^^^ CONCLUSION : MMP 1 , MMP 2 , and TIMP 2 , detected by immunohistochemistry are not helpful in predicting the prognosis of endometrial cancer patients . . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
In this study , the expressions of MT 1 MMP , MMP 2 and TIMP 2 were evaluated in 10 enchondromas , 34 conventional chondrosarcomas , 5 clear cell chondrosarcomas , 7 mesenchymal chondrosarcomas and 8 dedifferentiated chondrosarcomas . ^^^ The extent scores of MT 1 MMP , MMP 2 and TIMP 2 in grade 2 chondrosarcoma were significantly higher than those in either enchondroma or grade 1 chondrosarcoma ( P < 0 . 05 ) . ^^^ In conventional chondrosarcoma , significant correlations were found between the extent scores of MT 1 MMP and MMP 2 ( P < 0 . 001 ) , MT 1 MMP and TIMP 2 ( P < 0 . 01 ) , and MMP 2 and TIMP 2 ( P < 0 . 01 ) . ^^^ In dedifferentiated chondrosarcoma , the extent scores of MT 1 MMP , MMP 2 and TIMP 2 in low grade cartilaginous components were not significantly different from those in conventional chondrosarcoma ; however , the high grade anaplastic components showed high extent scores for MT 1 MMP , MMP 2 and TIMP 2 , compared with the low grade cartilaginous components of dedifferentiated chondrosarcoma or conventional chondrosarcoma . ^^^ According to our results , the expression of MT 1 MMP as well as that of MMP 2 or TIMP 2 demonstrated a significant correlation with the tumor grade in human cartilaginous tumors . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Activity of MMP 2 and MMP 9 by gelatin zymography and expression of MMP 2 , MMP 9 , TIMP 1 , and TIMP 2 mRNAs were examined in 11 lung cancer cell lines which included six small cell lung cancer ( SCLC ) cell lines . ^^^ Localization of MMP 2 , MMP 9 , TIMP 1 , and TIMP 2 was examined by immunohistochemistry in 43 resected NSCLC ( 22 adenocarcinomas , 17 squamous cell carcinomas , 4 large cell carcinomas ) using specific anti human monoclonal antibodies . ^^^ Expression of MMP 2 mRNA was detected in 5 ( 100 % ) , MMP 9 in 1 ( 20 % ) , TIMP 1 in 4 ( 80 % ) , and TIMP 2 in 5 ( 100 % ) of 5 NSCLC cell lines examined . ^^^ In 43 patients , MMP 2 , MMP 9 , TIMP 1 , and TIMP 2 immunoreactivity was demonstrated in 19 ( 44 % ) , 9 ( 21 % ) , 15 ( 35 % ) , and 29 ( 67 % ) excised tumors , respectively . ^^^ Both cancer cell lines and NSCLC tumor samples frequently expressed MMP 2 , MMP 9 , TIMP 1 , and TIMP 2 ; MMP 2 in particular was highly expressed in malignant cells and surrounding fibroblasts . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
TIMP 2 binds specifically and noncovalently to the pro form of MMP 2 and inhibits its enzyme activity . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
This paper describes the development , validation and application of a quantitative RT PCR ( Q RT PCR ) assay that allows the detection and quantitation of mRNAs encoding genes of three MMPs ( MMP 1 , MMP 2 , MMP 3 ) , three TIMPs ( TIMP 1 , TIMP 2 , TIMP 3 ) and GAPDH simultaneously from small amounts of RNA ( < 4 microg ) . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
MMP 2 ( 72 kDa gelatinase ) and tissue inhibitors TIMP 1 and TIMP 2 were detectable in vessels throughout the cycle . ^^^ MMP 1 , MMP 2 , MMP 3 , MMP 9 , TIMP 1 , and TIMP 2 had differing distributions and patterns of expression . ^^^ MMP 2 ( 72 kDa gelatinase ) and tissue inhibitors TIMP 1 and TIMP 2 were detectable in vessels throughout the cycle . ^^^ MMP 1 , MMP 2 , MMP 3 , MMP 9 , TIMP 1 , and TIMP 2 had differing distributions and patterns of expression . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
The expression of the latent forms of matrix metalloproteinase 2 ( MMP 2 ) , MMP 3 and MMP 9 , urokinase plasminogen activator ( uPA ) , tissue inhibitor of metalloproteinase 1 ( TIMP 1 ) and TIMP 2 expression were observed in both tumour and normal tissue samples from breast and colorectal tissue ; however , expression was greater in the tumour tissue . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
These studies examine gelatinase A , MT 1 MMP , and TIMP 2 synthesis by HSCs during activation in vitro and the potential role of gelatinase A in promoting HSC proliferation . ^^^ Gelatinase A , MT 1 MMP , and TIMP 2 messenger RNA ( mRNA ) were all upregulated in HSCs activated on plastic over 5 to 14 days . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Measurement of TIMP 1 , TIMP 2 , TIMP 3 , MMP 1 , and MMP 2 mRNA demonstrated no detectable change , whereas determination of mRNA for tissue transglutaminase , an enzyme capable of cross linking many ECM components , showed an eightfold increase ( P < 0 . 01 ) . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Using primary cultures of human myometrial smooth muscle cells we found that these cells express MMP 1 , MMP 3 , TIMP 1 and TIMP 2 mRNA and protein , with significantly higher values of TIMP than MMP . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
In this study , we show that transient adherence to fibronectin induces the production of activated forms of matrix metalloproteinase 2 ( MMP 2 ) and MMP 9 , as well as downregulation of tissue inhibitor of metalloproteinase 2 ( TIMP 2 ) by T cell lines . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Moderate MMP 2 and TIMP 2 RNA levels remained constant up to 144 hours after PH , and MMP 1 and 13 RNA were always undetectable . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
To clarify the significance of the balance between matrix metalloproteinase 2 ( MMP 2 ) and tissue inhibitor of metalloproteinase 2 ( TIMP 2 ) in the progression of renal cell carcinoma , we transfected both the MMP 2 and TIMP 2 genes simultaneously into RenCa , a mouse renal cell carcinoma cell line that does not express detectable levels of either MMP 2 or TIMP 2 mRNAs , and established several clones with various MMP 2 : TIMP 2 expression ratios . ^^^ On the basis of the quantitative evaluation of the MMP 2 : TIMP 2 mRNA expression ratio by Northern blot analysis , we selected a clone overexpressing MMP 2 alone ( RenCa / M ) , a clone overexpressing TIMP 2 alone ( RenCa / T ) , and two kinds of clones overexpressing both , i . e . , one with a high ( RenCa / MTh ) and one with a low ( RenCa / MTl ) MMP 2 : TIMP 2 ratio , to compare the tumor cell phenotypes . ^^^ In an in vitro tumor cell invasion assay , the MMP 2 : TIMP 2 ratios of the RenCa sublines were directly correlated with their invasive potential . ^^^ The invasive abilities of the parental RenCa cells induced by conditioned media from RenCa sublines were also correlated with the MMP 2 : TIMP 2 ratios of the sublines . ^^^ Furthermore , when injected i . v . or into the renal subcapsule in syngeneic mice , RenCa sublines formed metastatic nodules in the lungs , and the number of nodules was correlated with the MMP 2 : TIMP 2 ratio of each clone . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
TGF beta , metalloproteinases ( MMP 1 and MMP 2 ) and their inhibitors ( TIMP 1 and TIMP 2 ) were immunolocalized within fibroblasts , chondrocytes , and osteoblasts throughout the interface , indicating that signals modulating the osteogenic cell phenotype at these sites are highly regulated . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Enhanced expression of the type 4 collagenases MMP 2 and MMP 9 , or lack of their inhibitors TIMP 1 and TIMP 2 , has been associated with tumour invasion and metastatic potential in several experimental models . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
In this study , we investigated the in vitro and in vivo expression patterns of MMP 1 , MMP 2 , MMP 3 , MMP 9 , TIMP 1 and TIMP 2 mRNA and protein in a previously described human melanoma xenograft model . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Matrix metalloproteinase 2 ( MMP 2 , gelatinase A ) and its tissue inhibitor ( TIMP 2 ) are mainly known for their roles in the ( patho ) physiological remodeling of the vasculature , angiogenesis , tissue repair , tumor invasion , inflammation , and atherosclerotic plaque rupture . ^^^ Matrix metalloproteinase 2 ( MMP 2 , gelatinase A ) and its tissue inhibitor ( TIMP 2 ) are mainly known for their roles in the ( patho ) physiological remodeling of the vasculature , angiogenesis , tissue repair , tumor invasion , inflammation , and atherosclerotic plaque rupture . ^^^ In perfused rat mesenteric arteries with an intact endothelium , inhibition of vascular MMP 2 with TIMP 2 reduced ( by 16 . 2+ / 4 . 2 % ) the vasoconstrictor effects of big ET 1 ( 50 pmol ) . ^^^ However , when the endothelium was mechanically removed , TIMP 2 abolished ( > 90 % ) the vasoconstriction of big ET 1 , and this effect was mimicked by an anti MMP 2 antibody . ^^^ These data implicate , for the first time , the endogenous MMP 2 / TIMP 2 system in the regulation of vascular reactivity . . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Exposure of HPTC to 25 mM D glucose also led to the induction of tissue inhibitor of metalloproteinases ( TIMP 1 and TIMP 2 ) and also gelatinase A . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Positive IHC staining was evident for MMP 1 and 9 in 60 % to 70 % of tumor cells , and for MMP 11 , 13 , and 14 and TIMP 2 and 3 in 70 % to 100 % of tumor cells . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
On the contrary , at low concentration , i . e . 1 10 microg / ml , heparins stimulated the secretion of both 72 kDa gelatinase ( 1 . 4 1 . 6 fold ) and particularly TIMP 2 ( > 4 fold ) . ^^^ At high doses , MMP 2 and TIMP 2 production by fibroblasts returned to basal levels . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Localization and quantification of mRNA for matrix metalloproteinase 2 ( MMP 2 ) and tissue inhibitor of matrix metalloproteinase 2 ( TIMP 2 ) in human benign and malignant prostatic tissue . ^^^ MMP 2 and its ratio to TIMP 2 have been associated with tumor recurrence and progression in a number of human malignancies . ^^^ METHODS : We examined the relationship between MMP 2 and TIMP 2 mRNA expression in 42 men with malignant ( n = 32 ) and benign ( n = 10 ) prostates using nonisotopic in situ hybridization and Northern blot analysis . ^^^ RESULTS : mRNA for MMP 2 and TIMP 2 was localized to the malignant epithelial cells of both high and low grade tumors in the periphery of the glands and in areas of extracapsular involvement , and to the glandular epithelium in the benign prostates . ^^^ Using Northern blot analysis , the mean MMP 2 to TIMP 2 ratio was approximately one in the benign prostates and low grade and stage cancers . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Gelatinase A and TIMP 2 expression in the fibrous sclera of myopic and recovering chick eyes . ^^^ METHODS : Total RNA was extracted from the posterior scleras of form deprived chick eyes , eyes recovering from deprivation myopia , and paired contralateral control eyes , and subjected to northern blot analysis analyses using cDNA probes to chicken gelatinase A and TIMP 2 . ^^^ The distribution of gelatinase A and TIMP 2 mRNAs was evaluated by in situ hybridization on frozen sections of chick scleras using 33P labeled RNA probes . ^^^ CONCLUSIONS : Changes in the steady state levels of gelatinase A and TIMP 2 mRNA lead to changes in gelatinase activity within the fibrous sclera and mediate , at least in part , the process of visually regulated ocular growth and scleral remodeling . . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
To develop a RT PCR assay for evaluating gene expression concerning renin ( REN ) , angiotensinogen ( ATG ) and the following ECM components : transforming growth factor beta 1 ( TGF beta 1 ) , fibronectin ( FN ) , matrix metalloproteinase 2 ( MMP 2 ) and tissue inhibitor of metalloproteinases 2 ( TIMP 2 ) ; 2 . ^^^ To assess the influence of renal mass reduction ( RMR ) caused by subtotal ( 5 / 6 ) or partial ( 2 / 6 ) nephrectomy on gene expression for TGF beta 1 , FN , MMP 2 and TIMP 2 ; 3 . ^^^ Quantitation of renal gene expression for REN , ATG , TGF beta 1 , FN , MMP 2 and TIMP 2 was performed using RT PCR assay and comparing amounts of respective gene mRNA with house keeping gene mRNA encoding L 19 ribosomal protein . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Our data suggest that NO decreases MMP 2 and MMP 9 activities and increases TIMP 2 secretion , and this shifts the balance of MMP activity , which may favor the inhibition of cell migration because of inhibition of extracellular matrix degradation . . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Also , its relationship with MMP 2 and its inhibitor , TIMP 2 , was studied . ^^^ MMP 2 antisense ODN also induced similar changes , but they were relatively less ; on the other hand TIMP 2 antisense ODN induced a mild increase in the size of explants . ^^^ Treatment of TIMP 2 antisense ODN to metanephroi exposed to MT 1 MMP / MMP 2 antisense notably restored the morphology of the explants . ^^^ These findings suggest that the trimacromolecular complex of MT 1 MMP : MMP 2 : TIMP 2 modulates the organogenesis of the metanephros , conceivably by mediating paracrine / juxtacrine epithelial : mesenchymal interactions . . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
OBJECTIVE : We sought to determine the clinical significance of serum levels of TIMP 2 and MMP 2 in patients with SSc . ^^^ The TIMP 2 and MMP 2 levels were determined by means of sandwich enzyme linked immunosorbent assays . ^^^ The balance of TIMP 2 and MMP 2 may play an important role in patients with SSc . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
The identity of this proteinase as MMP 2 is confirmed by Western blotting , zymography , and inhibition of its activity by a specific inhibitor , TIMP 2 . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Purified CT PCPE inhibited MMP 2 with an IC ( 50 ) value much greater than that of TIMP 2 . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
We also show that H ras mediated invasiveness is significantly inhibited when the expression of MMP 2 is down regulated , using an oligodeoxyribonucleotide complementary to the MMP 2 mRNA , or when MMP 2 activity is blocked by its inhibitor TIMP 2 ( tissue inhibitors of matrix metalloproteinase 2 ) . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
The results indicate that the mesothelial cells and macrophages express MMP 1 ( collagenase 1 ) , MMP 3 ( stromelysin 1 ) , TIMP 1 and TIMP 2 mRNA and protein at various levels , with significantly higher TIMPs than MMPs , and higher MMP 1 than MMP 3 ( p < 0 . 001 ) . ^^^ There was a significant increase in secreted MMP 1 and TIMP 2 by mesothelial cells following transforming growth factor beta 1 treatments in a time dependent manner ( p = 0 . 05 and p = 0 . 01 ) , without affecting the secretion of these proteins by macrophages . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Aortic arch MMP 9 , tissue inhibitor of matrix metalloproteinase ( TIMP ) 1 , and TIMP 2 mRNA levels were reduced 29 % to 39 % , and MMP 2 mRNA levels increased . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
ELISA was used to assay MMP 2 , MMP 9 , TIMP 1 and TIMP 2 levels in the amniotic fluid . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
This induction of MMP 2 activity was correlated with Northern blot analysis , showing in HUVECs that IGF 2 increased the expression of MMP 2 mRNA , while it did not affect that of TIMP 2 , a tissue inhibitor of MMP 2 . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
It was the aim of this study to determine whether the expression of MMP 1 , MMP 2 , and TIMP 2 correlates with survival in patients with surgically treated cervical cancer stage IB . ^^^ RESULTS : MMP 1 , MMP 2 , and TIMP 2 were detected by immunohistochemistry in 74 % ( 113 / 154 ) , 32 % ( 49 / 154 ) , and 80 % ( 107 / 154 ) of the tumor samples , respectively . ^^^ Correlation coefficients for MMP 1 / MMP 2 , MMP 1 / TIMP 2 , MMP 2 / TIMP 2 were 0 . 14 ( p = 0 . 12 ) , 0 . 37 ( p = 0 . 0001 ) , and 0 . 17 ( p : 0 . 005 ) , respectively . ^^^ The expression of MMP 1 ( log rank test , p = 0 . 6 ) , MMP 2 ( log rank test , p = 0 . 8 ) , and TIMP 2 ( log rank test , p = 0 . 15 ) were not correlated with overall survival . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Compared with squamous cell carcinoma ( SqCC ) , adenocarcinoma ( AdC ) more frequently overexpressed MMP 1 , 11 , 13 , 14 , and TIMP 2 , and TIMP 1 and / or TIMP 2 overexpression positively correlated with more advanced stage disease . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
BACKGROUND : To clarify whether circulating matrix metalloproteinases ( MMPs ) and their tissue inhibitors ( TIMPs ) can be used as serum markers of fibroproliferation in chronic liver diseases , we studied the expression of MMP 2 and MMP 9 in relation to TIMP 1 and TIMP 2 in peripheral blood mononuclear leukocytes ( MNLs ) and polymorphonuclear leukocytes ( PMLs ) , and compared this expression to circulating concentrations and hepatic histology in patients with chronic active hepatitis C ( CAH ) . ^^^ The MMP 2 / TIMP 1 and MMP 9 / TIMP 1 ratios were lower in Ci patients than in healthy controls ; the MMP 2 / TIMP 2 and MMP 9 / TIMP 2 ratios were not different . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Outer membranes of chronic subdural haematoma were immunostained for MMP 1 , MMP 2 , MMP 9 , TIMP 1 and TIMP 2 . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Altogether , these data suggest that : ( 1 ) the balance between MMP 2 and TIMP 2 is important in human brain tumors ; and ( 2 ) TIMP expression may be a valuable marker for tumor malignancy . . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
RESULTS : Compared with normal conjunctival fibroblasts from six subjects , conjunctivochalasis fibroblasts from eight patients showed markedly increased transcript expression of MMP 1 ( 5 to 32 fold ) and MMP 3 ( 4 to 30 fold ) , whereas that of MMP 2 , TIMP 1 , TIMP 2 , and uPA was similar between the two groups . ^^^ There was increased caseinolytic activity of MMP 3 and collagenolytic activity of MMP 1 ( 2 . 2 fold ) by conjunctivochalasis fibroblasts , whereas no difference was noted between these two types of fibroblasts in the protein and gelatinolytic activity of MMP 2 or expression of TIMP 1 and TIMP 2 proteins , although that of TIMP 1 transcript was slightly higher in some conjunctivochalasis fibroblasts . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
In this study , the levels of MMP 2 and MMP 9 and their common tissue inhibitors of metalloproteinases ( TIMP 1 and TIMP 2 ) were measured in the cerebrospinal fluid ( CSF ) from patients with various meningitides including aseptic , fungal and tuberculous ones . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Alteration in expression of MMP 1 and MMP 2 but not TIMP 1 and TIMP 2 in hereditary gingival fibromatosis is mediated by TGF beta 1 autocrine stimulation . ^^^ Addition of antibodies against transforming growth factor beta 1 ( TGF beta 1 ) , which is produced in greater amounts by HGF fibroblasts , resulted in a slight increase in MMP 1 and a decrease in MMP 2 expression , whereas TIMP 1 and TIMP 2 expressions were unaffected . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
During HAEC repair in the presence of PAO 1 virulence factors , enhanced MMP 2 activation was associated with decreased rates of its specific inhibitor TIMP 2 , whereas enhanced MMP 9 activation was independent of changes of its specific inhibitor TIMP 1 . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
We have determined MMP 2 and MMP 9 and tissue inhibitors of MMPs ( TIMP 1 and TIMP 2 ) in blood plasma and in renal tissue samples of patients with renal cell carcinoma ( RCC ) from cancerous and non cancerous parts of the same kidney . ^^^ In plasma , MMP 9 concentrations were significantly higher in RCC patients than in healthy controls , MMP 2 and TIMP 2 concentrations were higher in healthy controls and TIMP 1 concentrations were not different . . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Immunohistochemical examination of the SE revealed that MMP 1 , 2 , 9 and TIMP 2 were expressed in macrophages and / or interstitium , similar to the findings in atheromatous plaque or PAU . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
In the present study , the expression of MMP 2 , 9 , and 14 ( membrane type MMP 1 ) and the production of tissue inhibitors of metalloproteinases ( TIMP 1 and TIMP 2 ) by cultured human cytotrophoblast cells from 6 to 11 wk of gestation were investigated . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Primary cultures of rat brain microvascular endothelial cells as well as the rat brain ( GP8 / 3 . 9 ) and rat retinal endothelial ( JG2 / 1 ) cell lines constitutively expressed MMP 2 , 9 and TIMP 2 . ^^^ In vitro activation of CNS endothelium with the pro inflammatory cytokines , tumour necrosis factor alpha and interleukin 1beta , resulted in selective upregulation of MMP 9 activity , whereas no significant changes were seen in MMP 2 or TIMP 2 levels at 24 h . ^^^ Quantitative differences found between CNS and non CNS endothelial cells in the expression of both MMP 2 and 9 , and in the expression of TIMP 2 demonstrate that CNS vascular endothelium is functionally distinct from non CNS endothelium . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
To elucidate the implication of type 4 collagenases ( MMP 2 and MMP 9 ) and their tissue inhibitors ( TIMP 1 and TIMP 2 ) for placental development , we quantified their levels in the conditioned media of placental organ culture and primary culture of the trophoblast as well as in the tissue extracts of placentas from different stages of gestation using specific enzyme linked immunosorbent assays . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
OBJECTIVE : To determine the effects of sulfasalazine ( SASP ) and its metabolites sulfapyridine ( SP ) and 5 amino salicylic acid ( 5ASA ) on steady state mRNA levels of inflammatory cytokines [ interleukin 1beta ( IL 1beta ) , tumor necrosis factor alpha ( TNF alpha ) ] , matrix metalloproteinases [ collagenase ( MMP 1 ) , stromelysin ( MMP 3 ) , gelatinase 72 kDa ( MMP 2 ) ] , tissue inhibitors of metalloproteinase ( TIMP 1 and TIMP 2 ) , and the TNF alpha receptor in rheumatoid synovial fibroblasts . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
PURPOSE : To determine the ability of radiation to modulate kidney tubule epithelial cell expression of various molecules involved in regulating extracellular matrix accumulation ( collagen types 1 and 3 , fibronectin , plasminogen activator inhibitor 1 ( PAI 1 ) , TGF beta and tissue inhibitor of metalloproteinases 2 ( TIMP 2 ) ) and degradation ( plasminogen activators u PA or t PA , MMP 2 and MMP 9 ) . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Expression levels of matrix metalloproteinases ( MMP 1 , MMP 2 , MMP 3 , MMP 7 , MMP 9 , MMP 10 , MMP 11 , MMP 13 , MT 1 MMP ) and tissue inhibitors of MMP ( TIMP 1 , TIMP 2 ) were evaluated by semiquantitative RT PCR , substrate zymography and western blot . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
The study also sought to determine the principal toxicities of BAY 12 9566 , whether plasma BAY 12 9566 steady state concentrations ( C ( ss ) ) of biologic relevance could be sustained for prolonged periods , and whether BAY 12 9566 affected plasma concentrations of MMP 2 , MMP 9 , and tissue inhibitor of MMP 2 ( TIMP 2 ) . ^^^ Plasma was collected to study the range of BAY 12 9566 C ( ss ) values achieved , and exploratory studies were performed to assess the effects of BAY 12 9566 on plasma concentrations of MMP 2 , MMP 9 , and TIMP 2 . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
For this study we developed and utilised specific polyclonal antibodies to assess the presence of collagenase ( MMP 13 ) , stromelysin 1 ( MMP 3 ) , gelatinase A ( MMP 2 ) , gelatinase B ( MMP 9 ) and TIMP 2 in both freshly isolated neonatal mouse calvariae and tissues cultured with and without bone resorbing agents . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Upregulation of MMP 2 , 8 , 9 , 13 , and 14 and TIMP 1 and TIMP 2 was detected at different timepoints during HF progression . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Interestingly , the vasodilator effects of thrombin were inhibited by a tissue inhibitor of MMP 2 ( TIMP 2 , 10 pmol ) . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
This was tested by determining the gene expression of MMP 1 , MMP 2 , and MMP 9 and respective TIMP 1 and TIMP 2 in luteal tissue from sows at the early , midluteal , and late luteal phase ( Days 6 8 , Days 9 11 , and Days 13 15 of estrous cycle ) . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
These suggest that overexpressions of MMP 2 and MT 1 MMP , loss of TIMP 2 expression , and up regulation of beta catenin by AAC 11 transfection may contribute to the development of cervical cancer invasion . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Type 1 angiotensin 2 receptor mRNA expression also correlated with matrix metalloproteinase 1 promoter region , tissue inhibitor of matrix metalloproteinase 2 ( TIMP 2 ) and tissue inhibitor of matrix metalloproteinase 3 mRNA expression . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Limited value of preoperative serum analyses of matrix metalloproteinases ( MMP 2 , MMP 9 ) and tissue inhibitors of matrix metalloproteinases ( TIMP 1 , TIMP 2 ) in colorectal cancer . ^^^ PURPOSE : We studied whether preoperative serum levels of free MMP 2 , the MMP 2 / TIMP 2 complex , and total amounts of MMP 9 , TIMP 1 and TIMP 2 correlated to the tumor stage and prognosis in colorectal cancer . ^^^ The patients as compared to the control group had significantly higher levels of free MMP 2 and total amounts of MMP 9 , TIMP 1 and TIMP 2 , whereas the level of the MMP 2 / TIMP 2 complex was significantly lower . ^^^ CONCLUSION : Serum analyses of free MMP 2 the MMP 2 / TIMP 2 complex and total amounts of MMP 9 , TIMP 1 and TIMP 2 are of limited value for tumor staging and prognosis in colorectal cancer . . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Free MMP 2 and that bound to the inhibitor , the MMP 2 / TIMP 2 complex were measured separately using different ELISAs . ^^^ Serum protein levels for TIMP 1 , TIMP 2 and the MMP 2 / TIMP 2 complex differed significantly in patients with lung cancer when compared to controls . ^^^ TIMP 1 levels were found to be higher in lung cancer than in controls , whereas TIMP 2 and MMP 2 / TIMP 2 complex levels were lower in lung cancer than in the sera of the control subjects . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
RT PCR analysis from cDNA of mouse A NK cells revealed mRNA for MMP 2 , MMP 9 , MMP 11 , MMP 13 , MT 1 MMP , MT 2 MMP , TIMP 1 , and TIMP 2 . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
The results illustrate the duality of TIMP 2 function , i . e . at low concentrations , TIMP 2 exerts a `` catalytic ' ' or enhancing effect on cell mediated proMMP 2 activation , whereas at higher concentrations , TIMP 2 inhibits the activation and / or activity of MMP 2 . . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
In the present study , the expression of MMP 2 , MMP 9 , TIMP 1 and TIMP 2 was determined by immunohistochemistry in right ventricular endomyocardial biopsy samples from 16 patients with idiopathic DCM , and its clinical significance was evaluated by comparison with parameters of cardiac function . ^^^ The left ventricular ejection fraction ( LVEF ) significantly correlated with the expression of both MMP 2 ( r= 0 . 68 ) and TIMP 2 ( r= 0 . 58 ) . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
METHODS : Levels of MMP 1 , MMP 2 , MMP 3 , MMP 7 , MMP 8 , MMP 9 , MMP 13 , TIMP 1 , and TIMP 2 in SF aspirated from knee joints of 97 patients with RA and 103 patients with OA were measured by the corresponding one step sandwich enzyme immunoassays . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
We examined expression of MMP 2 , MMP 9 , TIMP 1 , and TIMP 2 using immunohistochemistry and in situ reverse transcriptase polymerase chain reaction ( RT PCR ) . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Irrespective of whether MMP 2 was from endogenous or exogenous source there was an excellent correlation between the MT 1 MMP expression and degree of MMP 2 activation , whereas the gene expression of TIMP 2 was not significantly altered by high glucose concentration or concanavalin A . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Activation of matrix metalloproteinase 2 ( MMP 2 ) is mediated by binding to the complex of membrane type matrix metalloproteinase 1 ( MT 1 MMP ) with tissue inhibitor of MMP 2 ( TIMP 2 ) on the cell surface . ^^^ Binding of MMP 2 to integrin alpha ( 5 ) beta ( 3 ) has been implicated in presenting activated MMP 2 on the cell surface of invasive cells , but interactions with the MT 1 MMP TIMP 2 system have not been considered . ^^^ Therefore , we studied the expression and interaction of MT 1 MMP , MMP 2 and TIMP 2 in the alpha ( 5 ) beta ( 3 ) negative melanoma cell line BLM and in its beta ( 3 ) transfected , alpha ( 5 ) beta ( 3 ) expressing counterpart BLM beta ( 3 ) , both on cell lines and in xenografts . ^^^ Total expression levels of MMP 2 , MT 1 MMP and TIMP 2 did not differ markedly between the alpha ( 5 ) beta ( 3 ) negative and alpha ( 5 ) beta ( 3 ) positive cells . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
This activation of MMP 2 may be caused by MT 1 MMP of the cancer cells , since it was inhibited by a series of MMP inhibitors , including ethylenediaminetetraacetic acid ( EDTA ) , the tissue inhibitor of metalloproteinase 2 ( TIMP 2 ) , and the MMP inhibitor CGS 27023A , but not by TIMP 1 . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
This activation involves coordinated expression of MMP 2 , membrane type 1 MMP ( MT 1 MMP ) , and TIMP 2 . ^^^ Reverse transcription polymerase chain reaction ( RT PCR ) analysis of RNA isolated from subcutaneous xenografts revealed MT 1 MMP and TIMP 2 mRNA expression in all lesions , whereas MMP 2 mRNA could be detected only in xenografts derived from the highly metastatic cell lines 1F6m , MV 3 , and BLM . ^^^ Furthermore , immunohistochemistry demonstrated a marked increase of MMP 2 and MT 1 MMP in MV 3 and BLM xenografts , whereas TIMP 2 expression showed no evident correlation with metastatic capacity . ^^^ In human cutaneous melanocytic lesions , MMP 2 , MT 1 MMP , and TIMP 2 mRNA were detectable by RT PCR in all lesions . ^^^ Double staining experiments and immunohistochemistry on serial sections from the same lesions demonstrated that all tumour cells expressing MMP 2 also expressed MT 1 MMP and TIMP 2 . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
ELISA was used to screen conditioned medium for the production of MIF , matrix metalloproteinase 2 ( MMP 2 ) and tissue inhibitor of metalloproteinase 2 ( TIMP 2 ) . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
The aim of this study was to characterize the temporal changes in mRNA profiles for rat collagenase [ matrix metalloproteinase 1 ( MMP 1 ) ] , gelatinase A ( MMP 2 ) , matrilysin ( MMP 7 ) , gelatinase B ( MMP 9 ) , and membrane type 1 MMP ( MT 1 MMP ) , as well as tissue inhibitor of metalloproteinases 1 ( TIMP 1 ) , TIMP 2 , and TIMP 3 during the inflammatory , granulation , and early remodeling phases of excisional skin repair . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Immunofluorescence and quantitative immunofluorescence with antibodies against alpha smooth muscle actin , desmin , matrix metalloproteinases 2 ( MMP 2 ) , MMP 9 , tissue inhibitor of metalloproteinases 1 ( TIMP 1 ) and 2 ( TIMP 2 ) , urokinase type plasminogen activator ( u PA ) and its inhibitor 1 ( PAI 1 ) were studied with confocal microscopy . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
METHODS : Proteinase ( matrix metalloproteinase 1 ( MMP 1 ) , MMP 2 , MMP 3 , MMP 9 , urokinase type plasminogen activator ( uPA ) , and tissue type PA ( tPA ) ) and inhibitor ( tissue inhibitor of metalloproteinases ; TIMP 1 and TIMP 2 ) expression and proteinase activity were compared using substrate zymography , western blotting , immunohistochemistry , and quenched fluorescent substrate hydrolysis . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Expression of transcripts and proteins of MMP 1 , MMP 2 , MMP 3 , MMP 9 , TIMP 1 , TIMP 2 , and uPA was determined by Northern hybridization , ELISA , and Western blotting , respectively . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Imbalance of TIMP 2 / MMP 2 was observed using immunoprecipitation analysis in a glioma cell line . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
The conditioned medium of eEnd . 1 cells stimulated the release of MMP 2 and MMP 1 ( interstitial collagenase , EC 3 . 4 . 24 . 7 ) by endothelial cells , but not of the inhibitor TIMP 2 . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
We investigated whether the expression of membrane type 1 matrix metalloproteinase ( MT 1 MMP ) , matrix metalloproteinase 2 ( MMP 2 ) and tissue inhibitor of metalloproteinase 2 ( TIMP 2 ) was consistent with the proposed roles of these proteins in promoting metastasis in colorectal cancer . ^^^ Significant correlations were found between detection of MT 1 MMP and MMP 2 in tumour cell cytoplasm ( P < 0 . 05 ) , of MT 1 MMP and TIMP 2 in tumour cell cytoplasm ( P < 0 . 01 ) , and of MMP 2 and TIMP 2 in tumour cell cytoplasm ( P < 0 . 01 ) . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
To evaluate the spectrum and regulation of matrix metalloproteinases ( MMPs ) in bacterial meningitis ( BM ) , concentrations of MMP 2 , MMP 3 , MMP 8 , and MMP 9 and endogenous inhibitors of metalloproteinases ( TIMP 1 and TIMP 2 ) were measured in the cerebrospinal fluid ( CSF ) of 27 children with BM . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Among the changes described , striking features include increased expression of gelatinase A ( MMP 2 ) and membrane type 1 MMP ( MT ( 1 ) MMP ; MMP 14 ) as well as TIMP 1 and TIMP 2 . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
In addition , Western blotting results confirmed a higher expression of MMP 2 associated with a lower expression of TIMP 2 in HaCaT ras compared with HaCaT . ^^^ These results suggest that Ha ras oncogene could be a stimulating factor of migration and might modified the balance between MMP 2 and TIMP 2 in keratinocyte cell lines . . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Identification of immunohistochemically reactive MMP 2 , MMP 9 and TIMP 2 in BM tissue samples revealed for the first time that MMP 2 was localized in the myeloid cells , erythroblasts and megakaryocytes , MMP 9 in the myeloid cells and megakaryocytes . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Defective extracellular matrix reorganization by chronic wound fibroblasts is associated with alterations in TIMP 1 , TIMP 2 , and MMP 2 activity . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Melanoma cells may express a number of matrix metalloproteinase family members ( MMP 1 , MMP 2 , MMP 9 , MMP 13 , and MT 1 MMP ) as well as their tissue inhibitors ( TIMP 1 , TIMP 2 , and TIMP 3 ) . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
For this purpose , the presence and enzymatic activity of matrix metalloproteinases ( MMP 2 , MMP 9 ) , tissue inhibitors of MMPs ( TIMP 1 , TIMP 2 ) , urokinase type ( uPA ) and tissue type ( tPA ) plasminogen activators ( PAs ) , and plasminogen activator inhibitor 1 ( PAI 1 ) were quantified by western blot and gelatin or plasminogen casein zymography . ^^^ In addition , MMP 2 , TIMP 1 , TIMP 2 , and PAI 1 levels were measured by ELISA . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
MMP 1 and MT 1 MMP were detected in epithelial cells , and MMP 2 and TIMP 2 were detected in epithelial cells and some mesenchymal cells in each stage . ^^^ MMP 1 and MT 1 MMP were detected in epithelial cells , and MMP 2 and TIMP 2 were detected in epithelial cells and some mesenchymal cells in each stage . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
To elucidate possible mechanisms of phorbol 12 myristate 13 acetate ( PMA ) induced in vitro invasiveness of glioblastoma cells , we examined expression levels of membrane type 1 matrix metalloproteinase ( MT 1 MMP ) , MMP 2 , MMP 9 and tissue inhibitor of metalloproteinase ( TIMP ) 1 and TIMP 2 using Western blotting and gelatin zymography assay , and found that PMA induced the secretion of MMP 9 , activated MMP 2 proenzyme to fully active form of 59 kDa , down regulated the TIMP 1 and TIMP 2 secretion , and increased MT 1 MMP on the cell surface . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Expression of MMP 2 , TIMP 1 , and TIMP 2 mRNA and protein was not influenced by IL 1beta or TNF alpha , and no difference was found in the gelatinolytic activity of MMP 2 between both cell types . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
The gene expression of the gelatinase , MMP 2 and MMP 9 , and their respective inhibitors , TIMP 1 and TIMP 2 , in STS was evaluated . ^^^ Following mRNA isolation , reverse transcription polymerase chain reaction was performed using primers for MMP 2 , MMP 9 , TIMP 1 , and TIMP 2 . ^^^ Ratios of MMP 9 / TIMP 1 and MMP 2 / TIMP 2 gene expression as well as MMP 2 protein activation ratio ( active / inactive enzyme determined by gelatin zymography ) were analyzed for correlation with DM and DFS . ^^^ Relative levels of MMP 2 and MMP 9 , MMP2 / TIMP 2 ratio , and MMP 9 / TIMP 1 ratio were not significantly correlated with DM . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
METHODS : Gelatin zymography ( MMP 2 and MMP 9 ) , enzyme linked immunosorbent assays ( MMP 1 , MMP 3 , TIMP 1 and TIMP 2 ) and quenched fluorescent substrate hydrolysis ( total MMP activity ) were employed in resection specimens from 50 patients , four with adenomas and 46 with colorectal cancer . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
TIMP 2 is particularly important for control of MMP 2 and both have been implicated in many pathological processes from arthritis to tumour invasion . ^^^ Analysis of purified canine MMP 2 and MMP 9 showed that TIMP 2 is associated , and co purifies with MMP 2 . ^^^ This work highlights the importance of TIMP 2 as the main inhibitor for MMP 2 and , therefore , opens the possibilities of targeting TIMP 2 for therapeutic intervention against connective amino acid tissue degradation in a range of diseases . . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
A strong interaction between the TIMP 2 C domain ( Glu ( 153 ) Pro ( 221 ) ) and the gelatinase A hemopexin C domain ( Gly ( 446 ) Cys ( 660 ) ) was demonstrated by the yeast two hybrid system . ^^^ The MT 1 MMP hemopexin C domain did not form homodimers nor did it bind the gelatinase A hemopexin C domain , the C tail of TIMP 2 , or full length TIMP 2 . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
RT PCR analysis for gene expression of gelatinases and their respective inhibitors showed : a small increase in MMP 9 by week 1 , no change in TIMP 1 and MMP 2 , and a significant decrease in TIMP 2 by week 4 . ^^^ The increase in MMP 2 could be due to a decrease in TIMP 2 expression . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Expression of matrix metalloproteinases ( MMP 2 , MMP 9 , MT 1 MMP ) and their inhibitors ( TIMP 1 , TIMP 2 ) in common epithelial tumors of the ovary . ^^^ In this study , we investigated the immunohistochemical expression of MMP 2 , MT 1 MMP , TIMP 2 , MMP 9 , and TIMP 1 in 114 epithelial ovarian tumors ( 14 adenomas , 22 borderline tumors , and 78 adenocarcinomas ) . mRNA expression of MMP 2 , MT 1 MMP , and TIMP 2 was determined by RT PCR in selected samples . ^^^ The diffuse positive rates of MMP 2 , MT 1 MMP , TIMP 2 , and MMP 9 in ovarian carcinomas were significantly higher than those in the borderline and in benign tumors . ^^^ The percentages of the cases with triple diffuse positive expression for MMP 2 , MT 1 MMP , and TIMP 2 within the same tumor was significantly higher in malignant tumors than those in borderline and in benign tumors . ^^^ Considerable levels of mRNA expression of MMP 2 , MT 1 MMP and TIMP 2 were detected in all selected samples that showed triple diffuse positive immunostaining , confirming the co expression of MMP 2 , MT 1 MMP , and TIMP 2 at the transcriptional level within the same tumor . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
We have investigated the effects of tumor necrosis factor alpha ( TNF alpha ) and interferon ( INF gamma ) , the potent Bacillus Calmette Guerin ( BCG ) induced cytokines on the production of MMP 2 , MMP 9 , TIMP 1 , TIMP 2 and MT 1 MMP in high grade human bladder cancer cell lines , T 24 , J 82 and HT 1376 cell lines . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Specimens were examined for deposition of interstitial collagenase ( MMP 1 ) , stromelysin 1 ( MMP 3 ) , gelatinase A ( MMP 2 ) , gelatinase B ( MMP 9 ) , and three tissue inhibitors of metalloproteinases ( TIMP 1 , TIMP 2 , and TIMP 3 ) . ^^^ RESULTS : The results showed that unlike normal retina , which constitutively expresses MMP 1 and TIMP 2 , a large proportion of PDR membranes ( > 62 % ) stained for MMP 1 , MMP 2 , MMP 3 , MMP 9 , TIMP 1 , TIMP 2 , and TIMP 3 . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Both cell lines reacted by inducing the expression of the mRNAs encoding for MMP 1 , MMP 2 , TIMP 1 , and TIMP 2 , while other mRNAs did not vary ( MT 1 MMP , TIMP 3 ) or were not expressed ( MMP 9 ) . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
HGF / SF stimulated the expression of MMP 1 , 9 and MT 1 MMP and had a slight effect on expression of the MMP inhibitor TIMP 1 but not TIMP 2 . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
MMP 2 , and TIMP 1 and TIMP 2 were identified by gelatin zymography and reverse zymography , respectively . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
These fibroblasts also expressed mRNA for collagenase 1 ( CL 1 , MMP 1 ) , membrane type 1 matrix metalloproteinase , gelatinase A , gelatinase B , stromelysin 1 , stromelysin 2 , TIMP 1 , and TIMP 2 . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Immunohistochemical expression of matrix metalloproteinases 1 and 2 ( MMP 1 and MMP 2 ) and tissue inhibitor of metalloproteinase 2 ( TIMP 2 ) in ruptured and non ruptured tubal ectopic pregnancies . ^^^ To evaluate the expression of MMP 1 , MMP 2 , and the tissue inhibitor of MMP 2 ( TIMP 2 ) along the invasive pathway of trophoblast in ruptured and non ruptured tubal ectopic pregnancies , we performed a retrospective immunohistochemical study . ^^^ METHODS : In 15 tissue specimens of patients with ruptured ( N = 7 ) and non ruptured ( N = 8 ) first trimester tubal ectopic pregnancies who underwent laparoscopic salpingectomy , immunohistochemical staining against MMP 1 , MMP 2 , and TIMP 2 was performed . ^^^ Mean percentages of positive stained areas by MMP 1 , MMP 2 , and TIMP 2 antibodies in the extravillous trophoblast were determined for ruptured and non ruptured tubal ectopic pregnancies and compared . ^^^ RESULTS : In our 15 tissue specimens of ectopic pregnancies MMP 1 and TIMP 2 were found to be more prominent in the immunohistochemical distribution pattern than MMP 2 . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
PCR Southern blot analysis showed that the high magnitude cyclic tensile load increased the mRNA level of MMP 1 , MMP 3 , MMP 9 , IL 1beta , TNF alpha and TIMP 1 in the cultured chondrocytes , while the mRNA level of MMP 2 and TIMP 2 was unchanged . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
METHODS : Arterial tissue from four patients with aortic aneurysms and four age matched aortic samples was examined for the production and expression of MMP 2 , TIMP 2 and MT 1 MMP protein using immunohistochemistry , in situ hybridization and in situ zymography . ^^^ RESULTS : All components of the MMP 2 TIMP 2 MT 1 MMP enzyme system were detected in the arterial wall of both aneurysm and control samples , specifically in the medial tissue . ^^^ The localization of the MMP 2 TIMP 2 MT 1 MMP enzyme system to the medial layer of the arterial wall gives support to the concept that this system may play an aetiological role in the pathogenesis of AAAs . . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
It has been demonstrated that MMP 2 activation is dependent on the complex ( membrane type 1 [ MT 1 ] MMP / tissue inhibitor of MMP 2 [ TIMP 2 ] ) receptor and MMP 2 . ^^^ Reverse transcription polymerase chain reaction experiments showed the expression in platelets of messenger RNA encoding for MMP 2 , MT 1 MMP , and TIMP 2 . ^^^ Flow cytometry analysis showed that MT 1 MMP , MMP 2 , and TIMP 2 expressions were enhanced at the activated platelet surface . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
METHODS : The expression of MMP 2 and tissue inhibitor of metalloproteinase 2 ( TIMP 2 ) in cultured rat HSC was detected by immunocytochemistry ( ICC ) . ^^^ The relative amount of MMP 2 and TIMP 2 in the culture supernatant was assayed by ELISA and the activity of MMP 2 in supernatant was examined by zymography . ^^^ RESULTS : The expression of MMP 2 was enhanced in hypoxia group as compared with that in control one ( t ' 3 . 4685 , P < 0 . 05 ) , while the expression of TIMP 2 was decreased ( t ' =2 . 5673 , P < 0 . 05 ) . ^^^ Comparing the varied duration of hypoxia , the relative amount of MMP 2 protein was the highest in 6 h group ( 6h vs 12h , t=4 . 0945 , P < 0 . 001 ; 6h vs 24h , t=2 . 4652 , P < 0 . 025 ) , with a contrary curve in TIMP 2 . ^^^ CONCLUSION : Hypoxia promotes the expression of MMP 2 and inhibits the expression of TIMP 2 in HSC . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Using a nude mouse model of colorectal liver metastasis , we have overexpressed the MMP inhibitor , tissue inhibitor of MMP 2 ( TIMP 2 ) in the liver prior to , or following , tumor challenge by metastatic LS174T cells in vivo . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Serum MMP 1 , MMP 2 , MMP 9 , MMP 14 , and TIMP 1 and TIMP 2 levels were determined , and synovial tissue was examined by immunohistology for the expression of MMP 2 and MMP 9 , and their molecular regulators . ^^^ MMP 14 , TIMP 2 , and MMP 2 were all detected in similar areas of the lining layer on consecutive histologic sections . ^^^ In contrast , the expression of TIMP 2 , an inhibitor of MMP 2 , was lower in the RA than in the non RA samples ( 25 + / 12 versus 39 + / 9 cells / high power field ; P = 0 . 01 ) . ^^^ Synovial tissue expressions of MMP 2 , MMP 14 , and TIMP 2 were virtually undetectable in normal synovial tissue samples . ^^^ This may reflect augmented activation of MMP 2 by the relatively high levels of MMP 14 and low levels of TIMP 2 seen in these tissues . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
METHODS : We compared the biological characteristics , that is , growth rate , motility , and invasive activity , of five ovarian cancer cell lines with the gene expression of various matrix proteases ( matrix metalloproteinase 1 [ MMP 1 ] , MMP 2 , MMP 9 , membrane type MMP type 1 [ MT 1 MMP ] , MT 2 MMP , MT 3 MMP , urokinase plasminogen activator [ uPA ] ) , their inhibitors ( tissue inhibitor of metalloproteinase type 1 [ TIMP 1 ] , TIMP 2 , plasminogen activator inhibitor type 1 , [ PAI 1 ] , and PAI 2 ) , and the potential transcriptional regulators E1AF and Ets 1 . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Preincubation of these cells with SDF 1 stimulated the secretion of MMP 2 and MMP 9 in BM and PB CD 34 ( + ) cells but of TIMP 1 and TIMP 2 only in PB CD 34 ( + ) cells . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Basement membrane alterations in psoriasis are accompanied by epidermal overexpression of MMP 2 and its inhibitor TIMP 2 . ^^^ Here , we show evidence of a new mechanism involving basement membrane alterations accompanied by keratinocyte overexpression of matrix metalloproteinase ( MMP ) 2 and tissue inhibitor of MMP 2 ( TIMP 2 ) in both uninvolved and involved psoriatic skin . ^^^ Western blots showed a marked increase in a MMP 2 and TIMP 2 in uninvolved and involved psoriatic skin although it was more pronounced in the latter . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
To evaluate the role of matrix metalloproteinases ( MMPs ) in parenchymal remodeling in idiopathic interstitial pneumonia ( IIP ) , we studied MMP 2 and 9 activity , in bronchoalveolar lavage fluid ( BALF ) by zymography and the expression of MMP 2 and 9 and TIMP 2 in lung tissue by immunohistochemistry . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
High levels of MMP 2 , MMP 9 , MT 1 MMP and TIMP 2 mRNA correlate with poor survival in ovarian carcinoma . ^^^ The object of this study was to analyze the potential association between the expression of MMP 2 , MMP 9 , MT 1 MMP and TIMP 2 , and disease outcome in advanced stage ovarian carcinomas . ^^^ Comparable findings were observed in peritumoral stromal cells with ISH for MMP 2 , MMP 9 and TIMP 2 mRNA . ^^^ In univariate analysis of metastatic lesions , mRNA levels of TIMP 2 in stromal cells ( P = 0 . 031 ) , as well as for MMP 2 ( P = 0 . 027 ) and MT 1 MMP ( P = 0 . 008 ) in tumor cells , correlated with poor outcome . ^^^ We conclude that MMP 2 , MMP 9 , MT 1 MMP and TIMP 2 are valid markers of poor survival in advanced stage ovarian carcinoma . . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Expression of the genes for collagen IValpha 2 , collagen 3 , matrix metalloproteinase 2 , tissue inhibitor of metalloproteinases ( TIMP ) 1 and TIMP 2 , tenascin and transforming growth factor ( TGF ) beta 1 was studied by quantitative reverse transcriptase polymerase chain reaction . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
MMP 2 and 9 , and TIMP 2 were detected in gelatin and reverse zymographs , respectively , from extracts of implantation sites on each day studied . ^^^ In tissue sections the primary decidual tissue area stained consistently for TIMP 2 and on Days 7 and 8 this area also had positive staining for MMP 1 in close proximity to the implanting embryo . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Type 4 collagen mediated pro MMP 2 activation did not involve either a transcriptional modulation of MMP 2 , MT 1 MMP , or TIMP 2 expression nor any alteration of MT 1 MMP protein synthesis or processing . ^^^ An inverse relationship between MMP 2 activation and the concentration of secreted TIMP 2 was observed . ^^^ This is consistent with our previous report that TIMP 2 degradation is probably linked to the MT 1 MMP dependent MMP 2 activation mechanism . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
TGFbeta was shown to slightly induce the secretion of MMP 2 together with the mRNA for TIMP 1 , TIMP 2 , and , to a greater extent , TIMP 3 . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
MT 1 MMP , TIMP 2 , and the alphavbeta 3 integrin exhibited a similar pattern of staining , with pericellular patches that colocalized with either MMP 2 or caveolin 1 . ^^^ The presence of MT 1 MMP and TIMP 2 in caveolae patches could be seen only after treatment with concanavalin A , which induced MMP 2 activation but had no noticeable effect on the pattern or intensity of MMP 2 immunostaining . ^^^ In contrast , MMP 9 and TIMP 1 staining showed a pattern completely different from that of MMP 2 and TIMP 2 , with positive spots uniformly distributed throughout the cell body . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
The protein content of MMP 2 , MMP 9 , tissue inhibitor of metalloproteinase 1 ( TIMP 1 ) , and TIMP 2 was increased in high flow vein tissue by 21 % , 126 % , more than 100 fold , and 86 % , respectively . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
This process involves tissue inhibitor of MMP 2 ( TIMP 2 ) , forming an MT 1 MMP / TIMP 2 / pro MMP 2 complex . ^^^ We examined expression of MMP 2 , MT 1 MMP , and TIMP 2 of graft vessels using immunohistochemistry and protein level by western blot analysis . ^^^ In the allografts harvested at Days 22 , 28 , 40 , and 41 , MT 1 MMP was expressed in the endothelial cells and smooth muscle cells ( SMCs ) in media of some arteries without histological change , accompanied by expression of MMP 2 and TIMP 2 . ^^^ In the severely thickened neointima of the allograft harvested at Day 95 , MMP 2 and faint MT 1 MMP were expressed in SMCs of severely thickened neointima and media ; TIMP 2 expression was seen only in noncollagenous tissue of severely thickened neointima . ^^^ MMP 2 protein was more intensely expressed in the allograft harvested at Day 95 than in the allograft harvest at Day 41 , while TIMP 2 protein level was almost same in the 2 samples . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
RT PCR analysis from cDNA of rat A NK cells revealed mRNA for MMP 2 , MMP 9 , MMP 7 , MMP 10 , MMP 11 , MMP 13 , MT 1 MMP , MT 2 MMP , TIMP 1 , and TIMP 2 . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
CONCLUSIONS : Correlation was made at the mRNA and protein levels of TIMP expression compared with that of MMPs in a murine model of retinal NV , which suggests a temporal role for MMP 2 and 9 , MT 1 MMP , and TIMP 2 in new vessel formation in response to hypoxic stimulation . . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
There was no difference in the transcript and protein expression of MMP 2 , TIMP 1 , TIMP 2 , and uPA among these groups . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
This immunohistochemical study investigated the distribution pattern of matrix metalloproteinases ( MMP 1 , 2 , 3 , and 9 ) and their inhibitors [ alpha 2 macroglobulin ( alpha 2 M ) , tissue inhibitors of MMPs ( TIMP ) 1 , and TIMP 2 ] in human AA and AL amyloid deposits . ^^^ Only MMP 1 , 2 , and 3 , and alpha 2 M were present in AA amyloid deposits , and only TIMP 1 and TIMP 2 were found in deposits of AL amyloid . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Muscle damage was induced by downhill running ; furthermore , the exercise was performed at both cold temperature ( 5 degrees C ) and room temperature ( 22 degrees C ) to study also the possible effect of environmental temperature on serum concentrations of matrix metalloproteinases MMP 2 and MMP 9 , tissue inhibitors of metalloproteinases TIMP 1 and TIMP 2 , and MMP 2 / TIMP 2 complex , and muscle damage monitored by serum creatine kinase measurements . ^^^ The present study demonstrates an acute increase in serum concentrations of MMP 9 , TIMP 1 , and MMP 2 / TIMP 2 complex , but no changes in serum MMP 2 concentrations in response to eccentric exercise . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Expression of MMP 1 , MMP 2 , MMP 9 , TIMP 1 and TIMP 2 was assessed in 44 stage 2 NSCLC . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
We use dot blotting to analyse the expression of MMP 2 , MMP 9 and TIMP 2 in ACC 2 , a cell line of ACC in palate and ACC M , a high lung metastasis clone which cloned from ACC 2 . ^^^ The result showed the expression of MMP 2 and MMP 9 were higher in ACC M than that in ACC 2 , but the expression of TIMP 2 were higher in ACC 2 than that in ACC M This suggest MMP 2 and MMP 9 can promote ACC metastasis , TIMP 2 can suppress ACC metastasis . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
RESULTS : The addition of PDGF or TGF beta to the culture medium of keratoconic corneal keratocytes had no significant effect on overall protein production , MMP 2 activity or on the amounts of TIMP 1 and TIMP 2 secreted . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
MMP 2 and TIMP 2 were also immunolocalized to the carcinoma cells in the carcinoma samples . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Astrocytes expressed MMP 1 , MT 1 MMP , MMP 2 , TIMP 1 , and TIMP 2 in normal and glaucomatous ONH . ^^^ MMP 2 , TIMP 1 , and TIMP 2 localized to RGCs and their axons . ^^^ Cultured astrocytes constitutively expressed MMP 2 , MT 1 MMP , TIMP 1 , and TIMP 2 , whereas MMP 3 , MMP 7 , MMP 9 , and MMP 12 were not detectable in tissues or in cultured astrocytes . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Tissue inhibitors of metalloproteinase ( TIMP ) 1 and TIMP 2 were known to inhibit MMP 9 and MMP 2 , respectively . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
This study assessed the prognostic role of MMP 2 , MMP 9 metalloproteinases , and of the metalloproteinase inhibitor TIMP 2 , related to disease free survival ( DFS ) , in soft tissue sarcoma ( STS ) patients . ^^^ MATERIALS AND METHODS : Level and distribution of MMP 2 , MMP 9 , and TIMP 2 expression were evaluated on 73 biopsies by immunohistochemistry and immunoblotting . ^^^ RESULTS : In terms of DFS , increased MMP 2 reactivity and lack of TIMP 2 expression were significant for poor prognosis in all samples ( P = 0 . 0005 and P = 0 . 006 respectively ) . ^^^ Lack of TIMP 2 expression was a poor prognostic factor for DFS in synovial sarcoma ( P = 0 . 009 ) , while MMP 2 and MMP 9 correlated with metastasis ( P = 0 . 008 and P = 0 . 005 , respectively ) and grade ( P = 0 . 001 and P = 0 . 04 respectively ) in liposarcoma . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
The recombinant TIMP 4 was a potent inhibitor of gelatinase A ( apparent K ( 1 ) [ Ki ( app . ) ] < or = 9 pM ; k ( on ) ( association rate constant ) , 4 . 57 + / 0 . 13 10 10 ( 6 ) M ( 1 ) s ( 1 ) ) and was less dependent upon hemopexin C domain interactions than TIMP 2 in its mode of binding and inhibition . ^^^ In concanavalin A stimulated homozygous Timp 2 / fibroblasts or unstimulated MT 1 MMP transfected Timp 2 / cells , which can not activate progelatinase A , activation was restored by the addition of 0 . 3 5 nM TIMP 2 but not by TIMP 4 , unequivocally showing the TIMP 2 dependency of MT 1 MMP induced activation of gelatinase A and the fact that TIMP 4 can not support activation . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
MMP 2 is activated by membranous type 1 MMP ( MT 1 MMP ) and type 2 tissue inhibitor of MMP ( TIMP 2 ) . ^^^ High LPA suppressed the activation of MMP 2 through down regulation of MT 1 MMP and TIMP 2 . ^^^ C 3 and Y 27632 reversed the suppression of the activation of MMP 2 and expression of MT 1 MMP and TIMP 2 , suggesting the involvement of the Rho ROCK pathway in ECM degradation . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Expression of claudin 5 not only replaced TIMP 2 in pro MMP 2 activation by MT 1 MMP but also promoted activation of pro MMP 2 mediated by all MT MMPs and MT 1 MMP mutants lacking the transmembrane domain ( DeltaMT 1 MMP ) . ^^^ In contrast to the stimulatory effect of TIMP 2 on pro MMP 2 activation by MT 1 MMP , activation of pro MMP 2 by DeltaMT 1 MMP in the presence of claudin 5 and proDeltaMMP 2 processing by MT 1 MMP were both inversely repressed by expression of exogenous TIMP 2 . ^^^ These results suggest that TIMP 2 is not involved in cluadin 5 induced pro MMP 2 activation by MT MMPs . ^^^ TIMP 2 enhanced cell surface localization of MMP 2 mediated by MT 1 MMP , and claudin 1 also stimulated it . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
From this work , we propose that TIMP 2 may be engineered to abrogate MMP 14 binding , whereas its binding properties for other MMPs , including MMP 2 , are maintained . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Expression of MMP 2 , MMP 9 , MT 1 MMP , TIMP 1 , and TIMP 2 mRNA in valvular lesions of the heart . ^^^ This study was undertaken to investigate the mRNA synthesis of MMP 2 , MMP 9 , membrane type 1 ( MT 1 ) MMP , and matrix metalloproteinase inhibitors TIMP 1 and TIMP 2 by in situ hybridization in a set of heart mitral and aortic valves operatively removed due to degenerative or inflammatory valvular diseases . ^^^ The samples were studied by in situ hybridization with specific probes for MMP 2 , MMP 9 , MT 1 MMP , TIMP 1 , and TIMP 2 . ^^^ The signals for MMP 2 , MMP 9 , TIMP 1 , and TIMP 2 mRNA were localized in endothelial cells and in fibroblast like cells expressing alpha smooth muscle actin , thus showing myofibroblast type differentiation . ^^^ The results show that matrix metalloproteinases MMP 2 and MMP 9 , and matrix metalloproteinase inhibitors TIMP 1 and TIMP 2 mRNAs are synthesized in diseased valves and suggest that they may contribute to matrix remodelling in valvular disease . . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
RESULTS : Quantitative polymerase chain reaction results demonstrated an increased mRNA expression for MMP 2 , MMP 9 , and MT 1 MMP and a decreased expression for TIMP 2 in prematurely ruptured membranes compared with preterm labor membranes . ^^^ Enzyme linked immunosorbent assay documented increases in the amniotic fluid concentrations of immunoreactive and bioactive MMP 2 and MMP 9 and immunoreactive MMP 3 and a decreased TIMP 2 concentration in fluids obtained from the premature rupture of membranes group compared with the preterm labor group . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Expression of MMP 1 , MMP 2 , MMP 3 , MMP 7 , MMP 9 , MMP 10 , MMP 11 , MMP 13 , MMP 14 , TIMP 1 , and TIMP 2 was simultaneously analyzed in tissue homogenates using semiquantitative reverse transcription polymerase chain reaction assay . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Patients were evaluated for toxicity and tumor response , and blood and urine samples were taken for pharmacokinetics , bone resorption markers , direct targets of the inhibitor [ matrix metalloproteinase 2 ( MMP 2 ) , MMP 8 , and MMP 9 ] , indirect targets [ tissue inhibitor of metalloproteinase 1 ( TIMP 1 ) , TIMP 2 , basic fibroblast growth factor , vascular endothelial growth factor , vascular cell adhesion molecule 1 , soluble urokinase plasminogen activator receptor , and cathepsins B and H ] and for a tumor necrosis factor alpha cytokine release assay . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
CONCLUSION : The tumour type specific expression of the MMP 2 precursor in adenocarcinoma and lung metastases suggests that MMP 2 in the absence of TIMP 2 correlates with aggressive tumour progression and may serve as an indicator for poor prognosis . . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
MMP 2 , membrane type 1 MMP ( MT 1 MMP ) , and tissue inhibitor of metalloproteinases type 2 ( TIMP 2 ) expression were determined by Northern analysis . ^^^ SMCs were isolated from IMV , and the production and expression of MMP 2 and TIMP 2 in the SMC lines were quantified . ^^^ Tissue homogenates and isolated inferior mesenteric SMCs from patients with aneurysms demonstrated significantly elevated MMP 2 levels , with no difference in TIMP 2 or MT 1 MMP . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
We quantified tissue concentrations of MMP 2 and TIMP 2 in 65 malignant colorectal lesions and corresponding normal mucosa by enzyme linked immunosorbent assay , western blotting , and in situ hybridization . ^^^ Therefore an increased ratio of MMP 2 to TIMP 2 is strongly associated with advanced tumor stages , but a decreased ratio was observed in metastases . ^^^ These findings suggest that the MMP 2 : TIMP 2 ratio may prove useful as a marker of local invasion but not of metastasis in colorectal cancer . . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Leptin ( 10 40 ng / ml ) induced proliferation of the human umbilical vein endothelial cells ( HUVECs ) and elevation of MMP 2 , MMP 9 , TIMP 1 , and TIMP 2 expression in a dose dependent manner . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
DESIGN : Pleural effusion fluid and plasma concentrations of MMP 1 , MMP 2 , MMP 3 , MMP 8 , MMP 9 , TIMP 1 and TIMP 2 were determined by ELISA in 21 patients with TB pleuritis . ^^^ TB pleural fluid concentrations of MMP 1 , MMP 2 , TIMP 1 , and TIMP 2 were higher compared to TB plasma . ^^^ In TB pleural fluid vs TB plasma , the respective MMP 1 , MMP 2 , TIMP 1 , and TIMP 2 ratios were increased ( 0 . 46+ / 0 . 10 vs 0 . 17+ / 0 . 02 ; 25 . 2+ / 2 . 8 vs 4 . 2+ / 0 . 9 ; 139 . 0+ / 28 . 7 vs 27 . 8+ / 8 . 2 ; 0 . 67+ / 0 . 13 vs 0 . 18+ / 0 . 04 , P < 0 . 0005 each ) . ^^^ CONCLUSION : Compartmentalized MMP 1 , MMP 2 , TIMP 1 , and TIMP 2 and , compared to CHF , a surplus of MMP 1 , MMP 2 , MMP 8 , and MMP 9 in the pleural space obviously contribute to the fibrotic reactions in TB pleuritis . . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Both cell lines produced similar amounts of MMP 2 , MMP 9 and TIMP 2 . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
The results of Northern hybridisations were supported by immunohistochemical stainings of the corresponding tumour areas for MMP 2 , MMP 14 and TIMP 2 , further suggesting that these may have prognostic value for determining whether individual chondrosarcomas are locally aggressive or have a probability of recurrence . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
We here demonstrate that membrane type 1 ( MT 1 ) MMP , a physiological activator of proMMP 2 under TIMP 2 control , is present within the testis together with MMP 2 and TIMP 2 . ^^^ We further showed that the two cell lineages ( somatic and germinal ) expressed MT 1 MMP and TIMP 2 , whereas MMP 2 was of somatic origin . ^^^ We found that follicle stimulating hormone enhanced the expression of MMP 2 and TIMP 2 but not of MT 1 MMP , and promoted the activation of proMMP 2 . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
RESULTS : Concentrations of MMP 9 and MMP 2 were statistically higher in serous adenocarcinomas than in serous adenomas ( p < 0 . 01 , p < 0 . 05 , respectively ) , while the concentrations of TIMP 1 and TIMP 2 showed no significant difference between adenocarcinomas and adenomas . ^^^ The molar ratio of TIMP 2 / MMP 2 was lower in adenocarcinomas than in adenomas ( p < 0 . 05 ) . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Serum TIMP 1 , TIMP 2 , and MMP 1 in patients with systemic sclerosis , primary Raynaud ' s phenomenon , and in normal controls . ^^^ OBJECTIVE : To measure serum levels of tissue inhibitors of metalloproteinases , TIMP 1 , TIMP 2 , and collagenase 1 ( MMP 1 ) , in patients with diffuse cutaneous systemic sclerosis ( dcSSc ) , limited cutaneous systemic sclerosis ( lcSSc ) , primary Raynaud ' s phenomenon ( RP ) , and in normal controls . ^^^ METHODS : Serum samples from patients with dcSSc ( n=83 ) , lcSSc ( n=87 ) , RP ( n=80 ) , and normal controls ( n=98 ) were analysed using enzyme linked immunosorbent assays ( ELISAs ) for total TIMP 1 , TIMP 2 , and MMP 1 . ^^^ Serum TIMP 2 and MMP 1 levels in dcSSc and lcSSc did not differ significantly from those in normal controls . ^^^ No significant differences in serum TIMP 1 , TIMP 2 , or MMP 1 levels were shown in the RP group compared with normal controls . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
RNA was extracted from the peripheral blood lymphocytes and reverse transcriptase polymerase chain reaction ( RT PCR ) carried out for MMP 2 and TIMP 1 , TIMP 2 gene expression determination . ^^^ Supplementation of the diet with quercetin did not alter the MMP 2 or TIMP 2 gene transcription or plasma protein levels of the healthy subjects in this study . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
MMP 2 is constitutively secreted in a latent zymogen by osteoblasts , and requires the process of activation mediated by membrane type matrix metalloproteinase 1 ( MT 1 MMP ) / tissue inhibitor of metalloproteinase ( TIMP 2 ) complex in the cell surface . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Investigation of this layer in every resorption area by gelatin histozymography and TIMP 2 histochemistry demonstrates the presence of an MMP whose histozymographic activity is inhibited by such a low dose of the inhibitor CT 1746 as to identify it as gelatinase A or B . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
To investigate the antifibrogenetic effect of interferon alpha ( IFN alpha ) treatment on factors regulating hepatic fibrosis , serum MMP 1 , MMP 2 , TIMP 1 and TIMP 2 levels were measured by the one step sandwich enzyme immunoassay in 27 patients with chronic hepatitis C and compared with the histological status of the patients before and at the end of treatment . ^^^ In the NIF group , however , the MMP 1 / TIMP 1 ratio tended towards a decrease ; moreover , there was not only a significant increase in TIMP 2 levels but also a tendency towards an increase in TIMP 1 levels . ^^^ CONCLUSION : These results suggested that an elevated MMP 1 / TIMP 1 ratio may ameliorate liver fibrosis by interferon in cases of chronic hepatitis C , whereas elevated levels of TIMP 1 and TIMP 2 might impede improvement . . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
AGS cells secreted gelatinolytic ( prominently 90 kDa ) and caseinolytic ( 110 kDa ) activity together with MMP 1 , MMP 3 , and TIMP 1 , TIMP 2 , and TIMP 3 isoforms . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
The aims of our study were to characterize the presence and molecular weight forms of MMP 2 , 8 , and 9 and their specific inhibitor , tissue inhibitor of metalloproteinases ( TIMP ) 2 , in lungs of preterm infants during the early postnatal period and to determine whether levels of these MMPs and TIMP 2 in tracheal aspirate fluid ( TAF ) are associated with acute or chronic lung morbidity of the preterm infant . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
The results showed that MMP 1 and 9 were decreased , whereas TIMP 1 and 3 were increased , but there was no change in MMP 2 and 3 and TIMP 2 and 4 after LVAD support . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Indeed , Tiam 1 mediated Rac activation induced transcriptional up regulation of tissue inhibitor of metalloproteinases 1 ( TIMP 1 ) and post transcriptional up regulation of TIMP 2 , whereas secretion and activity levels of their counterparts , matrix metalloproteinase 9 and matrix metalloproteinase 2 , respectively , were not affected . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Swollen and / or elongated type 2 alveolar epithelial cells showed strong immunoreactions for MMP 2 , MT 1 MMP , and TIMP 2 . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
RESULTS : CD68+ macrophage infiltration and the expression of MIP 1alpha , MCP 1 , MMP 1 , MMP 3 , TIMP 1 , and TIMP 2 were observed in synovial tissue of patients with early RA . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
A role for the 72 kDa gelatinase ( MMP 2 ) and its inhibitor ( TIMP 2 ) in human parturition , premature rupture of membranes and intraamniotic infection . ^^^ MMP 2 degrades Type 4 collagen and TIMP 2 is its specific inhibitor . ^^^ The objective of this study was to determine if human parturition , rupture of membranes ( term and preterm ) and microbial invasion of the amniotic cavity ( MIAC ) are associated with changes in the concentrations of MMP 2 and TIMP 2 in amniotic fluid . ^^^ MMP 2 and TIMP 2 concentrations in amniotic fluid were determined using sensitive and specific immunoassays . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
In parallel to the development of LVH at B , gene expression was increased significantly for MMP 1 , MMP 2 , MMP 3 , and MMP 9 and for TIMP 1 and TIMP 2 and decreased significantly for TIMP 3 . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Altered expression of matrix metalloproteases ( MMPs ) and their inhibitors , the tissue inhibitors of matrix metalloproteases ( TIMPs ) , has been demonstrated in various tumour tissues . mRNA expression patterns of MMP 1 , MMP 2 , MMP 3 , MMP 9 , MMP 11 , MMP 12 , MMP 14 and TIMP 1 , TIMP 2 , TIMP 3 and TIMP 4 were evaluated by quantitative reverse transcriptase polymerase chain reaction ( RT PCR ) in 30 renal cell carcinomas ( RCC ) , as well as in the surrounding tissues . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Cellular activation of MMP 2 ( gelatinase A ) by MT 2 MMP occurs via a TIMP 2 independent pathway . ^^^ Untransfected cells did not activate endogenous or exogenous TIMP 2 free MMP 2 unless both TIMP 2 and concanavalin A ( ConA ) were added . ^^^ Transfected cells expressing hMT 2 MMP efficiently activated both endogenous and exogenous MMP 2 ( within 4 h ) via the 68 kDa intermediate in the absence of TIMP 2 and ConA . ^^^ In contrast , activation of MMP 2 by Timp 2 / cells expressing recombinant hMT 1 MMP occurred more slowly ( 12 h ) and required the addition of 0 . 3 27 nm TIMP 2 . ^^^ Addition of TIMP 2 or TIMP 4 did not enhance MMP 2 activation by MT 2 MMP at any concentration tested ; furthermore , activation was inhibited by both TIMPs at concentrations > 9 nm , consistent with the similar association rate constants ( k ( on ) ) calculated for the binding of TIMP 4 and TIMP 2 to MT 2 MMP ( 3 . 56 10 10 ( 5 ) m ( 1 ) s ( 1 ) and 6 . 52 10 10 ( 5 ) m ( 1 ) s ( 1 ) , respectively ) . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
EXPERIMENTAL DESIGN : Five microm , formalin fixed , paraffin embedded tissue sections from 153 RCCs were immunostained using specific antibodies against MMP 2 , MMP 9 , ( Novocastra , Burlingame , CA ) TIMP 1 , and TIMP 2 ( NeoMarkers , Fremont , CA ) proteins . ^^^ RESULTS : The rates of increased expression of MMPs and TIMPs in RCC were as follows : MMP 2 , 67 % ; MMP 9 , 43 % ; TIMP 1 , 46 % ; and TIMP 2 , 73 % . ^^^ CONCLUSION : Increased expression of MMP 2 , MMP 9 , TIMP 1 , and TIMP 2 proteins in RCCs correlate with poor prognostic variables including shortened patient survival . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Ovarian carcinoma cells in serous effusions show altered MMP 2 and TIMP 2 mRNA levels . ^^^ Sixty six effusions and 96 tissue sections were stained for MMP 1 , MMP 2 and MMP 9 applying immunohistochemistry ( IHC ) and analysed for MMP 2 , MMP 9 and TIMP 2 expression using mRNA in situ hybridisation ( ISH ) . ^^^ The levels were consistently higher in malignant cells , significantly so for MMP 1 ( P=0 . 016 ) and MMP 2 ( P=0 . 036 ) proteins , as well as for TIMP 2 mRNA ( P=0 . 008 ) . ^^^ In tissue sections , MMP 1 , MMP 2 and MMP 9 protein expression was mostly localised to tumour cells , while MMP 2 , MMP 9 and TIMP 2 mRNA were predominantly detected in stromal cells . ^^^ Adenocarcinoma cells in effusions showed a significant upregulation of MMP 2 expression compared with primary tumours , with a concomitant downregulation of TIMP 2 . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Type 4 collagenases matrix metalloproteinase 2 ( MMP 2 ) and MMP 9 and their related proteins , MT 1 MMP , tissue inhibitor of metalloproteinases 1 ( TIMP 1 ) , TIMP 2 , and TIMP 3 , are expressed during kidney morphogenesis and nephrogenesis , but the renal ontogeny of these proteins is only partially known , and their persistence in the adult remains controversial . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
KE 298 blocked this IL 1beta induced pro MMP 2 activation and MT 1 MMP expression , but did not affect IL 1beta induced tissue inhibitor of metalloproteinase 2 ( TIMP 2 ) secretion from rheumatoid synovial cells . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Furthermore , we have assessed the degradation pathway of collagen by measuring the mRNA and activity of myocardial MMP 2 and tissue inhibitor of metalloproteinase 2 ( TIMP 2 ) as well as the protein level of TIMP 2 . ^^^ It increased the mRNAs of colligin , collagen 1 and 3 , and of MMP 2 and TIMP 2 as well as MMP 2 activity in two phases : In the initial phase , at 3 and 4 days , the mRNA of colligin and of collagen 1 and 3 was elevated predominantly in the LV , MMP 2 and TIMP 2 mRNA , as well as TIMP 2 protein and MMP activity were increased in both ventricles . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
RESULTS : TNFalpha treatment increased MMP 1 , 3 , and 9 and TIMP 1 expression , whereas MMP 2 expression was not affected and TIMP 2 expression decreased . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Stromelysin 1 , gelatinase B , and collagenase 3 are induced during the early phase of acute liver injury associated with inflammation and increased necrosis / apoptosis , whereas gelatinase A , membrane type MMP , stromelysin 3 , metalloelastase , TIMP 1 , and TIMP 2 are essentially detectable during the recovery phase of liver injury corresponding to hepatocyte regeneration . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Recent evidence suggests that a complex of membrane type MMP ( MT 1 MMP ) and tissue inhibitor of metalloptroteinase 2 ( TIMP 2 ) participate in the activation of alphavbeta 3 associated MMP 2 . ^^^ We investigated whether alphavbeta 3 and MMP 2 are associated on the membranes of a human cell line , SiHa , and the possible involvement of MT 1 MMP and TIMP 2 in the modulation of MMP 2 activity . ^^^ Immunoblot assays show the expression of MT 1 MMP on SiHa cell membranes and secreted TIMP 2 and pro MMP 2 in the medium . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Gelatinase A TIMP 1 and TIMP 2 mRNA were not found in both cerebral aneurysms and normal cerebral arteries . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
In culture medium from cocultures we detected active forms of the matrix metalloproteinases MMP 1 , MMP 3 and MMP 9 accompanied by induction of gene expression of MMP 1 , membrane type 1 MMP ( MT 1 MMP ) and tissue inhibitor of metalloproteinase 2 ( TIMP 2 ) in chondrocytes . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
The expression of MMP 2 , MMP 9 , TIMP 1 and TIMP 2 was examined by immunohistochemistry and RT PCR . ^^^ RESULTS : MMP 2 , TIMP 1 and TIMP 2 immunoreactivities were expressed in normal corneas , predominantly in the corneal epithelium . ^^^ On day 35 , MMP 2 , TIMP 1 and TIMP 2 staining was seen only in corneal epithelium and vascular endothelial cells . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Localization of MMP 1 and its inhibitors , tissue inhibitor of metalloproteinase ( TIMP ) 1 and TIMP 2 , were investigated in lung tissue obtained from infants who died during different phases of BPD development . ^^^ In all studied cases ( n = 50 ) type 2 pneumocytes were found to be immunoreactive for MMP 1 , TIMP 1 , and TIMP 2 . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
MMP 2 co localized with MT 1 MMP ( its activating enzyme ) and TIMP 2 in neoplastic cells . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
The expression of colligin , collagen 1 and 3 , matrix metalloproteinases 2 ( MMP 2 ) , and tissue inhibitor of matrix metalloproteinase 2 ( TIMP 2 ) was examined by ribonuclease protection assay ( RPA ) and Western blotting . ^^^ The mRNA abundance of colligin , collagen 1 and 3 , MMP 2 , and TIMP 2 started to increase after 5 and 10 h , respectively , reaching a maximum after 20 30 h and decreasing thereafter . ^^^ The increase in collagen 3 and MMP 2 mRNA abundance and elevation of collagen 1 and 3 , and TIMP 2 protein was diminished by RpcAMPS . ^^^ The increase in collagen 1 , collagen 3 , MMP 2 , TIMP 2 , and colligin evoked by serum depletion was also diminished by PKA inhibition . . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Our investigation showed a net decrease of the matrix metalloproteinase MMP 2 expression and activity and an increase of its endogenous inhibitor TIMP 2 expression . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
There was only low expression of matrix metalloproteinase ( MMP ) 2 , which is mainly inhibited by TIMP 2 ; no expression of MMP 1 and MMP 3 in reactive lymphoid tissue ; and no expression of these MMPs in H / R S cells . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
METHODS : Reverse transcription polymerase chain reaction based measurements of the mRNA levels of MMP 2 , MMP 7 , MT 1 MMP , MT 3 MMP , TIMP 1 , and TIMP 2 in relation to the house keeping gene glyceraldehyde phosphate dehydrogenase were performed in cancerous and non cancerous prostatic tissue samples , in primary cell cultures of epithelial cells , in both fibroblasts , and smooth muscle cells as stromal cells , and in the human malignant prostatic cell lines DU 145 , LNCaP , and PC 3 . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Ginsenoside Rb 2 suppressed the expression and activity of MMP 2 , but did not alter the expression of tissue inhibitors of metalloproteinase ( TIMP ) 1 and TIMP 2 in the cells . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Precoating of the implant surface with type 1 collagen did not extensively affect proliferation , the activity of alkaline phosphatase , collagen synthesis , calcium accumulation , or the mRNA levels for collagen 1 alpha 1 , osteopontin , osteocalcin , MMP 2 , and TIMP 2 . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
OBJECTIVE : To investigate the relationship between the messenger RNA ( mRNA ) levels of matrix metalloproteinases ( MMP 9 , MMP 2 ) and tissue inhibitor of metalloproteinase ( TIMP 1 , TIMP 2 ) and malignant transformation of hydatidiform mole . ^^^ The mRNA expression of MMP 9 , MMP 2 , TIMP 1 , TIMP 2 genes were determined by RT PCR . ^^^ RESULTS : The differences of the mRNA expression of MMP 9 , MMP 2 , TIMP 1 , TIMP 2 genes in the tissues of hydatidiform mole and normal preganacy villus were not significant ( P > 0 . 05 ) . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Culture supernatants were assayed for MMP 1 , 2 , 3 , 7 , 9 , and 13 , and for tissue inhibitor of metalloproteinase 1 ( TIMP 1 ) and TIMP 2 , by enzyme linked immunosorbent assay . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
We have previously shown that synthetic MMP inhibitors work synergistically with TIMP 2 in the promotion of pro MMP 2 activation by MT 1 MMP in a process that depends on the affinity of the inhibitor toward MT 1 MMP . ^^^ Thus , in contrast to broad spectrum MMP inhibitors , the dithiols are less likely to promote MT 1 MMP dependent pro MMP 2 activation in the presence of TIMP 2 , while maintaining their ability to inhibit active MMP 2 effectively . . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Serum concentrations of interstitial collagenase ( MMP 1 ) , stromelysin 1 ( MMP 3 ) , gelatinase B ( MMP 9 ) , TIMP 1 and TIMP 2 were measured with an ELISA technique . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Western blots indicated that total MMP 2 secretion was inhibited and that TIMP 2 secretion was increased by DEX . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
An imbalance between the proteolytic activity of matrix metalloproteinase 2 ( MMP 2 ) and the tissue inhibitor of MMP 2 ( TIMP 2 ) is responsible for degradation of extracellular matrix ( ECM ) components and plays a critical role in tumor invasion and in metastasis formation . ^^^ Clinical role of MMP 2 / TIMP 2 imbalance in hepatocellular carcinoma . ^^^ We investigated the expression of MT 1 MMP in tissues , whereas both MMP 2 and TIMP 2 were evaluated in the sera and tissues ( primary and metastatic nodules ) of HCC patients with and without metastasis , whose clinical outcome was followed over a 2 year period . ^^^ Furthermore , we found a strict correlation between tissue and serum levels of TIMP 2 , this suggesting that a MMP 2 / TIMP 2 imbalance and in particular TIMP 2 levels , could represent an important prognostic factor in patients with HCC . . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Effect of avocado and soybean unsaponifiables on gelatinase A ( MMP 2 ) , stromelysin 1 ( MMP 3 ) , and tissue inhibitors of matrix metalloproteinase ( TIMP 1 and TIMP 2 ) secretion by human fibroblasts in culture . ^^^ In this study , the in vitro effects of avocado and soybean unsaponifiable residues ( ASU ) , their fractions ( avocado unsaponifiable [ ASF ] or soy unsaponifiable [ SSF ] ) on MMP 2 and MMP 3 , and the activity and secretion of their inhibitors TIMP 1 and TIMP 2 were investigated using cultured human gingival fibroblasts . ^^^ TIMP 1 , TIMP 2 , MMP 2 , and MMP 3 were also evidenced by dot blotting and quantified by image analysis . ^^^ As anticipated , IL 1beta induced a marked release of MMP 2 , MMP 3 , and TIMP 1 , but no variation for TIMP 2 was seen . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
On the other hand , tissue inhibitors of gelatinase activity ( TIMP 2 ) , are involved both in the MMP 2 activation process ; in concert with membrane type 1 MMP ( MT 1 MMP ) , and in the inhibition of gelatinolytic activity . ^^^ Therefore proteolysis , the central step for cancer metastasis , should occur as a result of an imbalance between MMP 2 and TIMP 2 . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
METHODS : Proteoglycan ( glycosaminoglycan ) and lactate quantification , Western immunoblot analyses of aggrecan degradation products , reverse transcription polymerase chain reaction analyses of aggrecanase 1 , aggrecanase 2 ( ADAM TS 4 , ADAM TS 5 , respectively ) , MMP 1 , MMP 3 , MMP 13 , tissue inhibitor of metalloproteinases 1 ( TIMP 1 ) , TIMP 2 , and TIMP 3 messenger RNA ( mRNA ) expression in articular cartilage explant cultures , and electrophoretic mobility shift assay analysis of nuclear factor of activated T cells ( NF AT ) transcription factor activation were used . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
METHODS : The expressions of MMP 2 , tissue inhibitor of matrix metalloproteinase 2 ( TIMP 2 ) and membrane type matrix metalloproteinase 1 ( MT 1 MMP ) in cultured rat HSC were detected by immunocytochemistry ( ICC ) and in situ hybridization ( ISH ) . ^^^ The contents of MMP 2 and TIMP 2 in culture supernatant were detected with ELISA and the activity of MMP 2 in supernatant was revealed by zymography . ^^^ In the situation of hyperoxia for 12h , the contents ( A ( 450 ) ) of MMP 2 and TIMP 2 in supernatant were both higher than those in the control , especially the TIMP 2 ( hyperoxia group : 0 . 0499 + / 0 . 0144 , n = 16 ; control : 0 . 0219 + / 0 . 0098 , n = 14 ; P < 0 . 01 ) , and so was the activity of MMP 2 ( hyperoxia group : 5 . 252 + / 0 . 771 , n = 14 ; control : 4 . 304 + / 1 . 083 , n = 12 ; P < 0 . 05 ) , and the expression of MT 1 MMP was increased . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
The expression of MMP 2 , MMP 9 , TIMP 2 , and type 4 collagen was investigated by immunohistochemical staining . ^^^ There was no significant correlation between phenotypes and expression of MMP 2 , TIMP 2 , or type 4 collagen . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
METHODS : RT PCR was used to assay the level of MMP 2 , MT 1 MMP , MMP 7 , TIMP 2 , TIMP 3 mRNA in 28 cases of colorectal cancer , including tumor tissue and surrounding normal tissue . ^^^ RESULTS : MMP 2 , MT 1 MMP , TIMP 2 and TIMP 3 were over expressed in tumor and normal tissues , and MMP 7 was strong expressed in tumor tissue but was weak expressed only in one case of normal tissue . ^^^ Inducing TIMP 2 , TIMP 3 or suppressing MMP 2 , MT 1 MMP , MMP 7 mRNA ' s expressions may inhibit the invasion and metastasis of cancer . . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Northern blot and nuclear run off assay showed that bFGF not only stimulated the expression of migration related matrix metalloproteinase 2 ( MMP 2 ) and tissue inhibitor of metalloproteinase 2 ( TIMP 2 ) but also enhanced the transcription of proliferation related osteopontin ( OPN ) and c jun genes in the quiescent VSMC . bFGF could moderately increase the expression of OPN and c jun , but had no significant effect on the expression of MMP 2 and TIMP 2 in the proliferating cells under the same conditions . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
However , AT2R mRNA directly correlated with transforming growth factor beta ( TGF beta ) ( r ( s ) 0 . 59 : p = 0 . 044 ) , matrix metalloproteinase 1 ( MMP 1 ) ( r ( s ) 0 . 83 ; p = 0 . 001 ) , tissue inhibitor of metalloproteinase 2 ( TIMP 2 ) ( r ( s ) 0 . 74 ; p = 0 . 001 ) and TIMP 3 ( r ( s ) 0 . 80 ; p = 0 . 001 ) mRNA expression . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Normal and reverse zymography , Western blot , and immunogold analyses of the vesicles showed two gelatinases , MMP 2 and MMP 9 , in both the active and proenzyme forms , the MT 1 MMP proenzyme located on the external side of the vesicle membrane and the two inhibitors TIMP 1 and TIMP 2 . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
METHODS : The tissue distribution of MMP 2 , MMP 9 , TIMP 1 , TIMP 2 and fibronectin was analysed by immunohistochemistry . ^^^ RESULTS : Apligraf expressed MMP 2 and MMP 9 and contained immunohistochemically detectable amounts of TIMP 1 and TIMP 2 . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Total cytoplasmic RNA was extracted from the cells , and the expression of MMP 2 and the tissue inhibitor of metalloproteinase 2 ( TIMP 2 ) mRNA was examined by Northern blot analysis . ^^^ Mechanical stretch also enhanced the expression of MMP 2 and TIMP 2 mRNA in scleral fibroblasts significantly . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
To examine the contribution of AT 1 receptor in the expression of matrix metalloproteinase 1 ( MMP 1 ) and its tissue inhibitor ( TIMP 2 ) in lipid deposited arterial tissues , New Zealand white rabbits were given high cholesterol chow ( with losartan 25 mg / d or vehicle ) for 10 weeks . ^^^ Losartan also significantly decreased the enhanced mRNA expression of MMP 1 and TIMP 2 in aortas of rabbits with high cholesterol diet . ^^^ Losartan treated rabbits revealed a reduction in immunohistochemical expression of MMP 1 , whereas TIMP 2 expression became localized to the intima . ^^^ These observations demonstrate that AT 1 receptor blockade with losartan reduces lipid deposition and exerts potent inhibitory effects on NF kappa B activation and modulates the expression of MMP 1 and TIMP 2 in hypercholesterolemic rabbits . . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
METHODS : levels of matrix metalloproteinases ( MMP 2 and MMP 9 ) and tissue inhibitor of metalloproteinases ( TIMP 1 and TIMP 2 ) were measured in the walls of medium sized ( 5 7 cm in diameter ) ruptured AAA ( rAAA ) ( n =30 ) and large ( > or = 7 cm in diameter ) asymptomatic AAA ( aAAA ) ( n=30 ) . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Protein expression of MMP 1 , MMP 12 , membrane type 1 MMP ( MT 1 MMP ) , tissue inhibitor of MMP 1 ( TIMP 1 ) , TIMP 2 , TIMP 3 , alpha actin , and beta actin was analyzed with electrophoresis on sodium dodecyl sulfate gels and immunoblotting . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Plasma levels of MMP 1 , MMP 2 , tissue inhibitor of MMP ( TIMP ) 1 and TIMP 2 were measured by enzyme linked immunosorbent assay . ^^^ Plasma MMP 2 / TIMP 2 ratio and MMP 2 activity in the coronary sinus showed significant increases 4 and 24 h after PTCA . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Matrix metalloprotease 2 ( MMP 2 ) and MMP 9 are proteolytic enzymes balanced by tissue inhibitor of MMP 2 ( TIMP 2 ) , commonly involved in cancer metastasis . ^^^ Immunostaining showed that in male cancer tissues MMP 2 and MMP 9 staining was more intense and diffuse than in female cancer tissues , while no differences were observed regarding TIMP 2 . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
We cloned two new Xenopus MMPs , gelatinase A ( MMP 2 ) and MT 3 MMP ( MMP 16 ) , and the MMP inhibitor TIMP 2 . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Binding to collagen 4 induced the expression and activation of matrix metalloproteinases A and B ( MMP 2 , MMP 9 ) , with a concomitant increase at the protein level of tissue specific inhibitors of metalloproteinases ( TIMP 1 , TIMP 2 ) . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
In support of this , blockade of MMP 2 activity by injection of TIMP 2 does not perturb circulation but does prevent glomerular assembly . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Inhibition of p38MAPK by the specific inhibitor SB 203580 resulted in downregulation of MMP 2 mRNA and protein levels as well as gelatinolytic activity , while expression levels of TIMP 1 and TIMP 2 mRNAs were not changed . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
METHODS : We analyzed the invasive activities of cervical carcinoma cell lines ( CAC 1 , CaSki , and SiHa ) and the gene expression of various matrix proteinases ( matrix metalloproteinase 1 [ MMP 1 ] , MMP 2 , MMP 9 , membrane type MMP type 1 [ MT 1 MMP ] , MT 2 MMP , and MT 3 MMP ) and their inhibitors ( tissue inhibitor of metalloproteinase type 1 [ TIMP 1 ] and TIMP 2 ) . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Expression of matrix metalloproteases ( MMP 2 , MT 1 MMP ) and their tissue inhibitor ( TIMP 2 ) by rat sertoli cells in culture : implications for spermatogenesis . ^^^ Here we report an analysis of MMP 2 , MT 1 MMP and TIMP 2 expression by rat Sertoli cells in culture using RT PCR and zymographic techniques . ^^^ In the case of TIMP 2 and MT 1 MMP , the respective transcripts were augmented up to three fold after 24 h of treatment , and MMP 2 transcripts increased by four times in the same period . ^^^ The results of this study suggest that : ( 1 ) at the surface of Sertoli cells pro MMP 2 activation mediated by MT 1 MMP may occur , involving TIMP 2 as a receptor ; and ( 2 ) the expression of these molecules is not constitutive in this cell type , but may be modulated by FSH and is therefore implicated in spermatogenesis . . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
This is evidenced by the fact that TENS at low magnitude : ( 1 ) inhibits recombinant human ( rh ) IL 1beta dependent induction of cyclooxygenase 2 ( COX 2 ) mRNA expression and production of prostaglandin estradiol ( PGE 2 ) ; ( 2 ) inhibits rhIL 1beta dependent induction matrix metalloproteinase 1 ( MMP 1 ) and MMP 3 synthesis by suppressing their mRNA expression ; ( 3 ) abrogates rhIL 1beta induced suppression of tissue inhibitor of metalloprotease 2 ( TIMP 2 ) expression ; and ( 4 ) reverses IL 1beta dependent suppression of osteocalcin and alkaline phosphatase synthesis . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
OBJECTIVE : ( 1 ) To investigate the effects of endothelin 1 ( ET 1 ) on cell proliferation of cultured human renal interstitial fibroblasts ( hRIFs ) , and the mRNA expression of type 1 Collagen ( Col 1 ) , transforming growth factor beta ( TGF beta ) , matrix metalloproteinase 1 ( MMP 1 ) and tissue inhibitors of metalloproteinase 1 , 2 ( TIMP 1 , TIMP 2 ) by hRIFs . ( 2 ) To investigate the changes of above effects after the hRIFs were pretreated with selective endothelin receptor type A antagonist ( ETaRA ) . ^^^ METHODS : ( 1 ) The proliferation of hRIFs was determined by MTT method . ( 2 ) The mRNA expression of Col 1 , TGF beta , MMP 1 , TIMP 1 and TIMP 2 was detected semiquantitatively with reverse transcription polymerase chain reaction ( RT PCR ) . ^^^ CONCLUSION : The stimulating effects of ET 1 on hRIFs proliferation and the mRNA expression of Col 1 , TGF beta , MMP 1 , TIMP 1 and TIMP 2 , and the inhibiting effects of ETaRA on the above effects suggest that ET 1 may participate in the process of renal interstitial fibrosis under pathological condition . . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
METHODS : In Dahl salt sensitive rats with hypertension , in which a stage of concentric , compensated left ventricular hypertrophy ( LVH ) at 11 weeks is followed by a distinct stage of congestive heart failure ( CHF ) with LV enlargement and dysfunction at 17 weeks , we determined protein and messenger ribonucleic acid ( mRNA ) levels of LV myocardial TIMP 2 and 4 and MMP 2 , as well as their concomitant activities . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Tissue inhibitor of MMP 2 ( TIMP 2 ) was identified as an inhibitor of MMP 2 and MT 1 MMP . ^^^ However , TIMP 2 was reported to be essential for cell mediated activation of MMP 2 , and thus the contribution of TIMP 2 to tumor invasion has remained controversial . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
As the tissue inhibitor of MMP 2 ( TIMP 2 ) is a regulator of gelatinase A activity , its concentration was measured by ELISA . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
AIMS AND METHODOLOGY : To examine the serial changes in the expression and activity of MMP 2 and expression of MT 1 MMP and tissue inhibitor of matrix metalloproteinase 2 ( TIMP 2 ) in rats after induction of pancreatitis by intraductal infusion of oleic acid , and to determine protein concentrations by Western blot analysis and localization of type 4 collagen by immunostaining . ^^^ TIMP 2 mRNA expression was significantly higher than that at time 0 throughout the study period , and gelatinolytic activity of active MMP 2 increased from day 3 to day 42 . ^^^ CONCLUSION : Our findings indicate that long term increases of gelatinolytic activity of active MMP 2 cause continuous disorganization of type 4 collagen in basement membranes during progressive atrophy of pancreatic gland in oleic acid induced pancreatitis , and that MT 1 MMP and TIMP 2 work as activating factors during proMMP 2 activation . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
The level of MMP 2 mRNA ( Northern blot ) and protein ( zymography , ELISA ) and the mRNA of its activator ( MT 1 MMP ) and inhibitor ( TIMP 2 ) were analyzed . ^^^ Short ( 6 hour ) hypoxia inhibited the mRNA expression of MMP 2 , MT 1 MMP , and TIMP 2 , culminating in reduced latent and active MMP 2 protein . ^^^ Prolonged ( 24 hour ) hypoxia further suppressed MT 1 MMP and TIMP 2 mRNA , whereas it enhanced MMP 2 mRNA and enzyme secretion ( after 48 hour hypoxia ) . ^^^ Reoxygenation did not influence the inhibited TIMP 2 but upregulated MMP 2 and MT 1 MMP mRNA expression , leading to enhanced secretion of active MMP 2 protein . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
We investigated whether PGF ( 2alpha ) also influenced the endogenous protein levels of tissue inhibitors of metalloproteinases , TIMP 1 and TIMP 2 , and matrix metalloproteinases , MMP 2 and MMP 9 , all of which have been implicated in remodeling of the extracellular matrix ( ECM ) . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Inhibition by anti MMP 2 and TIMP 2 define a functional role for MMP 2 in addition to the previously described function of MMP 9 . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
We identified and quantified proteases in the culture media of explants : MMP 1 ( collagenase 1 ) , MMP 2 and MMP 9 ( gelatinases A and B ) , MMP 3 ( stromelysin 1 ) , MMP 7 ( matrilysin 1 ) , and tissue inhibitors of metalloproteinases , TIMP 1 and TIMP 2 . ^^^ These data are in favor of an altered balance in anetodermic patients between MMP 2 and TIMP 2 . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
In situ hybridization showed that on day 1 4 of pregnancy , both MMP 2 and TIMP 2 mRNAs were evidently localized in the basal stromal cells . ^^^ On day 5 , MMP 2 mRNA signals were decreased in the basal stromal cells and mRNA for TIMP 2 was expressed in the epithelial cells and subepithelial stromal cells . ^^^ The weak mRNAs for MMP 2 , 9 , TIMP 1 , and 3 but not TIMP 2 were also observed in the ectoplacental cone / trophoblastic cells of the implanting embryos . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Activity of MMP 2 ( 72 kDa , gelatinase A ) and MMP 9 ( 92 kDa , gelatinase B ) was determined in the fluids by zymography and secretion of TIMPs ( 27 30 kDa ; TIMP 1 , glycosylated TIMP 3 and TIMP 4 , 24 kDa ; unglycosylated TIMP 3 , 21 kDa ; TIMP 2 ) by reverse zymography and quantified by densitometric analysis . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
We therefore determined serum concentrations of MMP 1 , MMP 3 , MMP 2 , MMP 9 and their inhibitors ( tissue inhibitors of metalloproteinase ) TIMP 1 , TIMP 2 and alpha 2 macroglobulin ( AMG ) in the serum of patients during the icteric stage of self limiting acute viral hepatitis . ^^^ Serum levels of TGF beta , IL 10 , MMP 1 , MMP 3 , MMP 2 , MMP 9 , TIMP 1 and TIMP 2 were assessed by ELISA . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
The expression of MT 1 MMP ( involved in MMP 2 activation ) was studied by Western blot analysis and RT PCR and the secretion of TIMP 2 by ELISA and RT PCR . ^^^ Myofibroblasts expressed high levels of MMP 2 , MT 1 MMP and TIMP 2 . ^^^ The hepatoma cell lines HepG 2 , HuH 7 and Hep3B did not express MMP 2 and weakly expressed TIMP 2 and MT 1 MMP . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
OBJECTIVE : To investigate the dynamic expression and its relation of gelatinase A ( MMP 2 ) , its natural inhibitor ( TIMP 2 ) and DNA index ( DI ) changes during carcinogenesis , invasion and metastasis in Wistar rats . ^^^ Immrno histochemistay ( IHC ) and in situ hybridigation were used in the monitor of MMP 2 , TIMP 2 proteins and mRNA expression during invasion and metastasis of lung cancer in these rats , DNA index ( DI ) value was measured by guantitatove image analysis on feulgen stained sections . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
On mRNA level , we observed high numbers of MMP 2 and TIMP 2 mRNA expressing iDC in MS . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
This study examined the effect of the preovulatory gonadotropin surge on localization and regulation of MMP 2 , MMP 14 , and tissue inhibitor of metalloproteinases 2 ( TIMP 2 ) mRNA and MMP 2 and TIMP 2 activity in bovine preovulatory follicles and new corpora lutea ( CL ) . ^^^ Messenger RNA for TIMP 2 and MMP 14 increased within 6 and 24 h of the gonadotropin surge , respectively , whereas MMP 2 mRNA was constitutively expressed . ^^^ Activity for MMP 2 in follicular fluid and follicle homogenates was not changed , but follicular fluid TIMP 2 activity increased in response to the gonadotropin surge . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
TIMP 2 is a potent inhibitor of MMPs , but it has also been implicated in a unique cell surface activation mechanism of latent MMP 2 / gelatinase A / type 4 collagenase ( proMMP 2 ) , through its binding to the hemopexin domain of proMMP 2 on the one hand and to a membrane type MMP activator on the other . ^^^ The present crystal structure of the human proMMP 2 / TIMP 2 complex reveals an interaction between the hemopexin domain of proMMP 2 and the C terminal domain of TIMP 2 , leaving the catalytic site of MMP 2 and the inhibitory site of TIMP 2 distant and spatially isolated . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
A series of reverse transcription polymerase chain reactions ( RT PCR ) were performed using MMP ( MMP 2 , MMP 7 , MMP 9 ) primers and TIMP ( TIMP 1 , TIMP 2 ) primers . ^^^ RESULTS : There is an increase in MMP 2 : TIMP 2 mRNA expression ratio in the normal and tumor margin tissue compared to the tumor . ^^^ CONCLUSIONS : Alterations exist in MMP 2 : TIMP 2 : expression at the margin , and all of the MMPs in the draining lymph nodes . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Evidence that MMP 2 and TIMP 2 are at play in the FSH induced changes in Sertoli cells . ^^^ Cell shape ( phase contrast microscopy ) and levels for MMP 2 ( gelatin zymography ) and its inhibitor TIMP 2 ( Northern and Western blot ) were monitored . ^^^ Cytochalasin D mimicked FSH , and MMP 2 production and TIMP 2 gene expression were augmented . ^^^ Interestingly , TNFalpha reversed FSH and cytochalasin D induced effects both on cell shape and on MMP 2 and TIMP 2 . ^^^ MMP 2 and TIMP 2 are likely involved in the FSH induced changes in Sertoli cells . . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Zymography of culture media was used to assess MMP activity , and Western blot analysis was used for quantification of MMP 2 and tissue inhibitor of MMP 2 ( TIMP 2 ) secretion . ^^^ CONCLUSION : Dexamethasone inhibits rat aortic smooth muscle cell migration , MMP 2 activity , and MMP 2 secretion and increases TIMP 2 secretion . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
In addition , we evaluated the changes in the levels of MMP 2 and MMP 9 as well as tissue inhibitors of MMPs ( TIMP 1 and TIMP 2 ) released by cultured human umbilical vein endothelial cells in response to VEGF ( 0 . 1 10 ng / mL ) . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
METHODS : Using primary bronchial epithelial cells MMP 2 , MT 1 MMP and TIMP 2 mRNA expression were assessed by Northern blotting and RT PCR . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
The aim of this study was to investigate quantitative mRNA expression of matrix metalloproteinases MMP 1 , MMP 2 , MMP 9 , and their inhibitors , the tissue inhibitors of metalloproteinases TIMP 1 , TIMP 2 and TIMP 3 , in vaginal wall tissue from women with stress urinary incontinence compared to continent controls . ^^^ Quantitative competitive reverse transcription ( QC RT PCR ) was carried out with oligonucleotide primers to quantify MMP 1 , MMP 2 , MMP 9 , TIMP 1 , TIMP 2 and TIMP 3 mRNA expression . ^^^ There was no difference in TIMP 2 , TIMP 3 , MMP 2 or MMP 9 mRNA expression between stress incontinent women and controls . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
This study investigated the direct effects of human OPG on cathepsin K , MMP 9 , MMP 2 , and tissue inhibitors of metalloproteinases ( TIMP 1 and TIMP 2 ) expressed by purified rabbit osteoclasts . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
We determined the levels of MMP 2 , MMP 9 , TIMP 1 and TIMP 2 in the bone marrow of 37 patients with acute myelogenous leukaemia ( AML ) and 18 patients with acute lymphoblastic leukaemia ( ALL ) before chemotherapy . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
The present study examined the expression and activation of two specific members of MMPs ( MMP 2 and MMP 9 ) and their related inhibitors ( TIMP 1 and TIMP 2 ) in an experimental retinal ischemia reperfusion injury . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Pericellular activation of gelatinase A ( MMP 2 ) proceeds via the formation of a complex involving its inhibitor , TIMP 2 , its activator ( s ) , MT MMPs and alphavbeta 3 integrin forming a docking system . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
They are secreted in a latent form and activated at the cellular surface by a membrane type 1 MMP ( MT 1 MMP ) and a tissue inhibitor of MMP 2 ( TIMP 2 ) that is also responsible for striking a balance between the proteolytic enzymes and TIMP 2 . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
TIMP 2 expression is high in SHEP / Bcl 2 cells , which likely inhibits MMP 2 activity , and absent in SHEP / N Myc / Bcl 2 cells , allowing MMP 2 activity . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
The 72 kDa protease activity has been found to be inhibited by tissue inhibitor of metalloprotease 2 ( TIMP 2 ) , indicating that the protease is the matrix metalloprotease 2 ( MMP 2 ) . ^^^ Under both basal and ONOO triggered conditions , the MMP 2 activity and the Ca2+ ATPase activity were also inhibited by EGTA , 1 : 10 phenanthroline , and TIMP 2 . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Plasma and tissue were analyzed for cAMP , MMP 2 and MMP 9 , and TIMP 1 and TIMP 2 . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
RESULTS : ( 1 ) Tripterine suppressed the development of proteinuria , decreased the level of serum anti dsDNA antibodies , reduced the expressions of collagen type 4 , fibronectin , TIMP 1 , TIMP 2 , TGF beta ( 1 ) and improved the expressions of MMP 1 , 2 in the murine kidney . ( 2 ) The use of Tripterine before occurrence of proteinuria had more obvious protective effects than its use after the occurrence of proteinuria . ( 3 ) No significant difference was found between the 3 mg / kg / week Tripterine treated group and the 6 mg / kg / week Tripterine treated group . ( 4 ) No obvious change was observed in the expression of MMP 3 . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
OBJECTIVES : To detect the level of matrix metalloproteinase 2 ( MMP 2 ) mRNA and the tissue inhibitor of metalloproteinase 2 ( TIMP 2 ) mRNA in bladder transitional cell carcinoma ( BTCC ) , and to estimate the prognosis for bladder tumor based on the quality and quantity of MMP 2 and TIMP 2 mRNA . ^^^ The positive rates of MMP 2 and TIMP 2 mRNA were 71 . 4 % and 65 . 7 % in BTCC , and 66 . 7 % and 60 . 0 % in the normal bladder wall . ^^^ Similarly , the MMP 2 to TIMP 2 ratio also showed statistically significant difference between normal bladder tissue and bladder carcinoma ( P < 0 . 01 ) . ^^^ The MMP 2 to TIMP 2 ratio may play a more significant role in the determination of the aggressiveness and prognosis of bladder tumor . . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
In addition , PMA induced downregulation of TIMP 1 and TIMP 2 secretion and upregulation of the membrane type 1 MMP , a major activator of MMP 2 on the cell surface , were reversed by SB 203580 in these cells ; the PMA induced increase of invasion in vitro decreased when SB 203580 was added to the top compartment of a modified Boyden chamber ; and the inhibitor also reduced the MMP secretion and PMA induced in vitro invasion in various glioma cell lines . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
The concentrations of MMP 1 , MMP 2 ( pro ) , MMP 2 ( active ) , MMP 8 , MMP 9 and TIMP 2 were measured in detergent extracts of the biopsy homogenates using ELISAs and gelatin zymography . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
BACKGROUND : In malignant melanoma , recent studies have demonstrated an important role of matrix metalloproteinase 2 ( MMP 2 ) , its co activating enzyme membrane type matrix metalloproteinase 1 ( MT 1 MMP ) , and the endogenous inhibitor of MMP 2 , tissue inhibitor of matrix metalloproteinase 2 ( TIMP 2 ) . ^^^ MMP 2 , TIMP 2 and MT 1 MMP are differentially expressed in lesional skin of melanocytic nevi and their expression is modulated by UVB light . ^^^ OBJECTIVE : To clarify the role of MMP 2 , TIMP 2 and MT 1 MMP in UVB irradiated vs . non irradiated melanocytic nevi . ^^^ METHODS : Immunohistochemical comparison of the MMP 2 , TIMP 2 and MT 1 MMP expression pattern . ^^^ The reduction of TIMP 2 expression in melanocytic cells by UV light together with the enhanced expression of MMP 2 in the adjacent epidermis may promote basement membrane degradation . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
In 38 patients with MS , MMP 2 and TIMP 1 levels were similar before and after 9 months of IFN beta therapy , whereas MMP 9 levels significantly decreased and TIMP 2 levels significantly increased in comparison to values obtained before treatment . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
MMP 1 , MMP 9 and TIMP 2 tended to be positive only in the atheromatous lesions . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
In addition , the balance of activity between MMP 2 , MMP 9 , and TIMP 2 may be relevant to carcinoma invasion and metastasis , including liver metastases in pancreatic and colon carcinoma . . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Elevated glucose concentrations resulted in decreased expression of MMP 9 and MMP 2 , whereas an increase in TIMP 1 and a decrease in TIMP 2 expression were observed . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
We hypothesized that there is an imbalance between the expression of TIMP 2 and the expression of MMP 2 and MT 1 MMP that favors activation of MMP 2 in malignant colon tumors compared to normal colonic tissue . ^^^ MMP 2 , MT 1 MMP , and TIMP 2 RNA transcripts were measured in each sample using a quantitative reverse transcriptase polymerase chain reaction assay . ^^^ In addition , the TIMP 2 : MMP 2 ratio was twofold lower ( P = 0 . 001 ) and the TIMP 2 : MT 1 MMP ratio was 1 . 5 fold lower ( P = 0 . 003 ) in tumors compared to normal mucosa . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
The activation process requires multimolecular complex formation involving pro MMP 2 , membrane type 1 MMP ( MT 1 MMP ) , and tissue inhibitor of metalloproteinases 2 ( TIMP 2 ) . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Melanoma cells expressing alpha 5 beta 3 , but not those expressing alpha IIb beta 3 or no beta 3 integrin , produced the active form of metalloproteinase MMP 2 and expressed elevated mRNA levels of MT 1 MMP and TIMP 2 . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
RESULTS : MMP 2 , MMP 3 , MMP 7 , MMP 9 , MMP 13 , MT 1 MMP and TIMP 2 mRNA was detected in the tibial articular chondrocytes of STR / ort mice at all ages ( 12 , 18 , 24 , 30 and 35 weeks ) . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Migration in 3D collagen was decreased by TIMP 2 ( an inhibitor of MT 1 MMP ) , but not by TIMP 1 ( a poor inhibitor of MT 1 MMP , but an efficient inhibitor of MMP 2 ) . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Because TIMP 2 inhibits MMP 2 preferentially , we then examined glioma growth in MMP 2 deficient mice and observed essentially identical consequences . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
The goal of the study was to evaluate the plasma blood concentration of VEGF , bFGF , MMP 1 , MMP 2 , MMP 3 , MMP 8 , MMP 9 , TIMP 1 , and TIMP 2 in patients with cancer and in normal subjects . ^^^ In conclusion , predominance of MMP 2 over TIMP 2 and TIMP 1 over MMP 1 as well as increased concentration of bFGF in peripheral blood are common features in patients with thyroid cancer . . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
The disproportional increase in MT 1 MMP and TIMP 2 might contribute to MMP 2 activation . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Since 72 kDa is the molecular mass of MMP 2 and since in our present study the 72 kDa protease in the gelatin containing zymogram is inhibited by matrix metalloprotease inhibitors , EGTA and TIMP 2 , it may be suggested that the 72 kDa protease is the MMP 2 . ^^^ Adding MMP 2 to the smooth muscle plasma membrane suspension caused an increase in Ca2+ATPase activity and pretreatment with TIMP 2 prevents the increase in Ca2+ATPase activity . ^^^ Pretreatment with TIMP 2 prevents the increase in Ca2+ATPase activity elicited by the low doses of MMP 2 and / or t buOOH . . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
The results showed that MMP 2 mRNA was only expressed at the early stages of pregnancy ; TIMP 2 mRNA was highly expressed at the early and late pregnancy and day 1 postpartum , but could not be detected during the mid phase of pregnancy ; TIMP 3 mRNA expression was abundant during early pregnancy and peaked at day 7 , but was absent from other time points examined . ^^^ Taken together , our results suggest that MMP 2 and TIMP 3 may have functional roles in rat luteal formation , while TIMP 2 may be implicated in both formation and regression of the pregnant CL . . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
RESULTS : After the hemodialysis process , MMP 9 , MMP 2 and TIMP 2 levels were 124 + / 72 , 706 + / 242 and 248 + / 90 ng / mL , significantly different from the pre HD values ( 187 + / 148 , 759 + / 304 and 43 + / 14 ng / mL ) . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
METHODS : Immunohistochemistry was used to determine the expression of proteinases ( MMP 1 , MMP 2 , MMP 3 , MMP 9 , urokinase type PA , and tissue type PA ) and inhibitors ( TIMP 1 and TIMP 2 ) in 44 patients with breast cancer . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
RESULTS : ( 1 ) An important release of inhibitors over low levels of active enzymes was observed in controls independently of age and sex except for higher TIMP 1 levels in males . ( 2 ) In cancer patients , increased pro MMP 9 and active MMP 2 with reduced TIMP 2 levels correlated with higher stages and histological grades . ( 3 ) Conversely , reduced MMP 9 and lipocalin levels were initial hallmarks of clinical relapses . ^^^ CONCLUSIONS : The imbalance between increased MMP 2 , 9 and decreased TIMP 2 levels appears to be linked to tumor stage and grade and , more importantly , to clinical events . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
OBJECTIVE : To investigate expression of matrix metalloproteinase 2 ( MMP 2 ) , membranous type 1 matrix metalloproteinase ( MT 1 MMP ) , and tissue inhibitor of metalloproteinase 2 ( TIMP 2 ) in ectopic and eutopic endometrium from women with and without endometriosis throughout the menstrual cycle . ^^^ RESULT ( S ) : Uterine endometrium from women with endometriosis expressed higher levels of MMP 2 and MT 1 MMP and lower levels of TIMP 2 than did endometrium from normal women . ^^^ CONCLUSION ( S ) : Eutopic endometrium from patients with endometriosis may be more invasive and prone to peritoneal implantation because of greater expression of MMP 2 and MT 1 MMP and lower expression of TIMP 2 messenger RNA , compared with endometrium from women without endometriosis . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Tissue inhibitor of metalloproteinase ( TIMP ) 4 binds pro matrix metalloproteinase ( MMP ) 2 and efficiently inhibits MT 1 MMP , but unlike TIMP 2 neither forms a trimolecular complex nor supports pro MMP 2 activation . ^^^ Both C TIMP 4 and C TIMP 2 bound pro MMP 2 and blocked concanavalin A induced cellular activation of the enzyme . ^^^ Measurement of k ( on ) rates revealed that the inhibition of MMP 2 by TIMP 4 is preceded by a C domain docking interaction , but in contrast to TIMP 2 , this is not enhanced by a C terminal tail interaction and so occurs at a slower rate . ^^^ Indeed , the binding stability of C TIMP 4 was unaltered by deletion of the C terminal tail , but replacement with the tail of TIMP 2 increased its affinity for pro MMP 2 by approximately 2 fold , as did substitution with the TIMP 2 C terminal tail acidic residues in the tail of C TIMP 4 ( V193E / Q194D ) . ^^^ Conversely , substitution of the C terminal tail of C TIMP 2 with that of TIMP 4 reduced pro MMP 2 binding by approximately 75 % , as did reduction of its acidic character by mutation to the corresponding TIMP 4 amino acid residues ( E192V / D193Q ) . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Furthermore , TSA inhibits cytochalasin D induced activation of gelatinase A , but TSA does not affect other members of the gelatinase A activation complex , MT 1 MMP and TIMP 2 . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
OBJECTIVE : To examine the in vitro invasiveness of synoviocytes obtained from inflamed joints of patients with arthritis in relation to the expression of MMP 1 14 , 17 , 19 , cathepsin K , the tissue inhibitors of matrix metalloproteinases TIMP 1 and TIMP 2 by FLS . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
MMP 1 , MMP 2 , TIMP 1 , TIMP 2 , and urokinase type PA levels in the distribution of the LAD were higher in the laser group than in the control or sham group . ^^^ MMP 1 , MMP 2 , TIMP 1 , TIMP 2 , and urokinase type PA levels in the distribution of the LAD were higher in the laser group than in the control or sham group . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
The invasive front was graded for malignancy , and immunohistochemical staining with MMP 2 , MMP 9 , TIMP 1 and TIMP 2 antibodies was performed . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
The purpose of this study was to quantify MMP 2 , MMP 9 , TIMP 1 and TIMP 2 in human seminal plasma and to evaluate their association with sperm . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
METHODS : The expression of MMP 2 , tissue inhibitor of matrix metalloproteinase 2 ( TIMP 2 ) and membrane type matrix metalloproteinase 1 ( MT 1 MMP ) in cultured rat HSC under hypoxic or hyperoxic conditions were detected with immunocytochemistry ( LSAB method ) , the contents of MMP 2 , TIMP 2 in culture supernatant with ELISA and the activity of MMP 2 in supernatant with zymography . ^^^ RESULTS : ( 1 ) In the situation of hypoxia for 12 h , the expression of MMP 2 increased ( hypoxia group positive indexes : 5 . 7 + / 2 . 0 ; control : 3 . 2 + / 1 . 0 ; P < 0 . 01 ) , while TIMP 2 decreased ( hypoxia group positive indexes : 2 . 5 + / 0 . 7 ; control : 3 . 6 + / 1 . 0 ; P < 0 . 05 ) in HSC , and the activity of MMP 2 in supernatant declined obviously ( hypoxia group : 7 . 334 + / 1 . 922 ; control : 17 . 277 + / 7 . 424 ; P < 0 . 01 ) . ^^^ At the different time courses of hypoxia , the change of expression and activity of MMP 2 was most notable at 6 h . ( 2 ) In the situation of hyperoxia for 12 h , the protein contents of MMP 2 , TIMP 2 in supernatant were both higher than those of the control , especially the TIMP 2 ( hyperoxia group A ( 450 ) : 0 . 050 + / 0 . 014 ; control : 0 . 022 + / 0 . 010 ; P < 0 . 01 ) , and so was the activity of MMP 2 ( hyperoxia group total A : 5 . 252 + / 0 . 771 ; control : 4 . 304 + / 1 . 083 ; P < 0 . 05 ) . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Reverse transcriptase polymerase chain reaction , zymography and reverse zymography for MMP 2 , MMP 9 , TIMP 1 , TIMP 2 , and TIMP 3 demonstrated a shift of the proteolytic antiproteolytic balance upon addition of VSMCs . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Activation of the latent MMP 2 zymogen involves its binding to the cell surface MT 1 MMP * TIMP 2 ( membrane type 1 matrix metalloproteinase / tissue inhibitor of matrix metalloproteinase 2 ) complex with subsequent cleavage of proMMP 2 by TIMP 2 free adjacent MT 1 MMP . ^^^ To observe the MMP 2 activation pathway in more detail , proMMP 2 deficient MCF 7 breast carcinoma cells expressing MT 1 MMP were incubated with excess proMMP 2 to saturate the available MT 1 MMP * TIMP 2 surface receptors . ^^^ The proteolytically competent mature MMP 2 moiety alone , but not in its complex with TIMP 2 , was released from the cells . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Expression of MMP 2 , TIMP 2 protein and the ratio of MMP 2 / TIMP 2 in gallbladder carcinoma and their significance . ^^^ AIM : To study the correlation between expression of MMP 2 , TIMP 2 protein and the ratio of MMP 2 / TIMP 2 and clinical pathological parameters of patients with gallbladder carcinoma . ^^^ METHODS : Carcinomas ( n=45 ) and polypoid lesions ( n=15 ) of the gallbladder were studied for the expression of MMP 2 and TIMP 2 protein by immunohistochemical avidin biotin complex method and image analysis . ^^^ RESULTS : There was significant difference between the average level ( 1 . 123+ / 0 . 108 vs 1 . 030+ / 0 . 054 , P=0 . 002 ) of MMP 2 , the ratio ( 1 . 050+ / 0 . 013 vs 0 . 937+ / 0 . 078 , P=0 . 003 ) of MMP 2 / TIMP 2 in gallbladder carcinomas and in polypoid lesions of the gallbladder . ^^^ MMP 2 / TIMP 2 ratio did not correlate with histological type , grade of differentiation and liver invasion , but significant differences were found between MMP 2 / TIMP 2 ratio and different Nevin stage , infiltration level and lymph node involvement in patients with carcinoma of gallbladder . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Modulation of monocytes matrix metalloproteinase 2 , MT 1 MMP and TIMP 2 by interferon gamma and beta : implications to multiple sclerosis . ^^^ In the present study , the effects mediated by IFN gamma and IFN beta on the mRNA and protein expression of MMP 2 , its physiological activator , MT 1 MMP and its endogenous inhibitor , TIMP 2 , by monocytes were evaluated in vitro . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Expression of MMP 2 , MMP 9 , and MMP 1 and their endogenous counterregulators TIMP 1 and TIMP 2 in postmortem brain tissue of Parkinson ' s disease . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Samples were assayed for plasma MMP 2 , tissue inhibitors of matrix metalloproteinase 2 ( TIMP 2 ) and IL 6 concentration . ^^^ Differential regulation of MMP 2 , TIMP 2 and IL 6 in valve replacement versus CABG patients . ^^^ CONCLUSION : Plasma MMP 2 concentrations reflect the state of the left ventricle , and changes in plasma MMP 2 and TIMP 2 concentrations during CPB may play an important role in LV remodeling after cardiac surgery . . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
The results showed that DEP exposure induced : 1 ) a net dose dependent decrease in CSK stiffness through actin fibers , 2 ) a concomitant specific reduction of both alpha ( 3 ) and beta ( 1 ) integrin subunits extensively expressed on the HBE cell surface , 3 ) a decrease in the level of CD 44 , which is a major HBE cell cell and HBE cell matrix adhesion molecule ; and 4 ) an isolated decrease in MMP 1 expression without any change in tissue inhibitor of matrix metalloproteinase ( TIMP ) 1 or TIMP 2 tissue inhibitors . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
E . coli expression of TIMP 4 and comparative kinetic studies with TIMP 1 and TIMP 2 : insights into the interactions of TIMPs and matrix metalloproteinase 2 ( gelatinase A ) . ^^^ Molecular interactions of TIMPs and MMPs investigated by IAsys biosensor analysis highlighted different modes of interaction between proMMP 2 TIMP 2 ( or TIMP 4 ) and active MMP 2 TIMP 2 ( or TIMP 4 ) complexes . ^^^ The observation that both active MMP 2 and inactive MMP 2 ( with the active site blocked either by the propeptide or a hydroxamate inhibitor ) have essentially identical affinities for TIMP 2 suggests that there are two TIMP binding sites on the hemopexin domain of MMP 2 : one with high affinity that is involved in proMMP 2 or hydroxamate inhibited MMP 2 ; and the other with low affinity involved in formation of the complex of active MMP 2 and TIMP 2 . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
This study was designed to investigate the expression of MMP 2 , MMP 9 and TIMP 1 , TIMP 2 in human squamous cell carcinomas of uterine cervix , and the association with invasion and metastasis of human squamous cell carcinoma of uterine cervix . ^^^ RESULTS : Immunohistochemical staining of tumor cells for MMP 2 , MMP 9 , TIMP 1 , and TIMP 2 were noted 82 . 5 % , 70 . 0 % , 62 . 5 % , and 97 . 5 % in carcinomas , 44 . 8 % , 62 . 1 % , 86 . 21 % , and 96 . 55 % in CIN lesions respectively , for MMP 2 , MMP 9 , TIMP 1 in 25 . 0 % normal cervices , but for TIMP 2 in 56 . 25 % normal cervices . ^^^ The positive rates of MMP 2 and MMP 9 in lymph node metastasis were obviously higher than that in without lymph node metastasis ( P < 0 . 01 ) , but for TIMP 2 was contrary and there was no significant difference for TIMP 1 . ^^^ CONCLUSIONS : The over expression of MMP 2 , MMP 9 , TIMP 1 , and TIMP 2 may play a key role in invasion and lymph node metastasis of in squamous carcinoma of the cervix . . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
METHODS : Expression of MMPs and their tissue inhibitors ( TIMPs ) in human aortic , mitral , tricuspid and pulmonary valves from unused donor or transplant recipient hearts was determined by immunohistochemical staining using antibodies against human MMP 1 , MMP 2 , MMP 3 and MMP 9 and their inhibitors TIMP 1 , TIMP 2 , TIMP 3 . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
RESULTS : CsA simultaneously stimulated TGF beta 1 expression and production and inhibited expression of MMP 1 and MMP 2 by human gingival fibroblasts , whereas CsA has a slight effect on TIMP 1 and TIMP 2 expression . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
METHODS : Reverse transcription polymerase chain reaction ( RT PCR ) with specific primers for MMP 1 , MMP 2 , MMP 9 , TIMP 1 and TIMP 2 was performed on tissue specimens obtained from patients with cataract , rhegmatogenous retinal detachment , glaucoma , or diabetes mellitus . ^^^ MMP 2 , TIMP 1 and TIMP 2 were detected in 25 ( 96 . 2 % ) of the 26 samples with GAPDH expression , while MMP 1 and MMP 9 were detected with a lower percentage ( 34 . 6 and 19 . 2 % , respectively ) . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
RESULTS : Northern analysis revealed overexpression of MMP 2 , MMP 9 , and TIMP 2 mRNAs in subcutaneous tumors of HPDt hamsters as compared with normal pancreatic tissue . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
METHODS : Immunocytochemistry and zymography techniques were used to measure the MMP 2 , MMP 9 , TIMP 1 , TIMP 2 protein levels and activities in endometrial carcinoma tissue of 37 patients and control group composed of 7 normal postmenstrual endometrial samples . ^^^ RESULTS : The MMP 2 , MMP 9 , TIMP 1 and TIMP 2 proteins mainly expressed in endometrial carcinoma cells , glandular cells and endothelial cells . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
This study was designed to evaluate the relationship between the expression of MMP 2 , MMP 9 , and TIMP 2 and biological behavior of ovarian cancer . ^^^ METHODS : The expression of MMP 2 , MMP 9 , and TIMP 2 in 128 patients with ovarian tumor was determined using immunohistochemical methods , and their relationship to many clinicopathological parameters was analyzed . ^^^ RESULTS : Immunohistochemical studies revealed a diffuse granular distribution of MMP 2 , MMP 9 , and TIMP 2 in the cytoplasm of the carcinoma cells . ^^^ The expression of MMP 2 , MMP 9 , and TIMP 2 was stronger in ovarian adenocarcinoma tissue than in ovarian benign tumor tissue ( P < 0 . 01 ) . ^^^ TIMP 2 expression was contrary to the expression of MMP 2 and MMP 9 on that three respects in ovarian carcinomas . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
RESULTS : Treatment with TNFalpha increased MMP 1 , 3 , and 9 and TIMP 1 , whereas expression of MMP 2 was not affected and expression of TIMP 2 was decreased . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Among MMP 1 , 2 , 3 , 7 , 8 , 9 , and 13 , TIMP 1 and 2 measured by their sandwich enzyme immunoassay systems , the production of MMP 1 and MMP 3 but not TIMP 1 or TIMP 2 was significantly enhanced by the treatment of cultured OA chondrocytes with VEGF ( P < 0 . 05 ) , whereas no such effect was obtained with cultured NOR chondrocytes . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
In this group of patients there were reductions in MMP : TIMP ratios , in particular the MMP 1 : TIMP 1 ratio in the LL ( p = 0 . 05 ) , MMP 3 : TIMP 1 ratio in the LL ( p = 0 . 05 ) and SL ( p = 0 . 008 ) , and MMP 3 : TIMP 2 ratio in the LL ( p = 0 . 04 ) and SL ( p = 0 . 08 ) . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
The aggressive mechanism of pituitary adenomas was studied through investigating the expression of MMP 9 , MMP 2 , TIMP 1 , and TIMP 2 in both invasive and non invasive adenomas . ^^^ Immunohistochemistry staining ( SP method ) was used to detect the expression of MMP 9 , MMP 2 , TIMP 1 , and TIMP 2 in two groups . ^^^ RESULTS : Immunohistochemical staining of tumor cells for MMP 9 , TIMP 1 , MMP 2 , and TIMP 2 were noted 95 . 9 % ( 47 / 49 ) , 57 . 1 % ( 28 / 49 ) , 75 . 5 % ( 37 / 49 ) and 89 . 8 % ( 44 / 49 ) in invasive adenomas , and 100 % ( 12 / 12 ) , 91 . 7 % ( 11 / 12 ) , 66 . 7 % ( 8 / 12 ) , and 91 . 7 % ( 11 / 12 ) in non invasive adenomas , respectively . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
These proteins existed as complexes of proMMP 9 / TIMP 1 , proMMP 2 / TIMP 2 , MMP 2 / TIMP 2 , free TIMP 1 , and TIMP 2 in human seminal plasma . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Therefore , experiments were designed to compare aggregation , dense granular ATP release , expression of MMPs ( MMP 2 , MMP 9 , and MMP 14 ) and tissue inhibitors of metalloproteinase ( TIMP 1 and TIMP 2 ) in circulating platelets from sexually mature ( 7 mo old ) gonadally intact and ovariectomized ( 4 wk ) female pigs . ^^^ In platelet lysates , active MMP 2 increased , whereas MMP 14 significantly decreased , after ovariectomy ; the expression of TIMP 1 , TIMP 2 , and P selectin did not change . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Western blotting was used to identify a specific MMP , MMP 2 ( a gelatinase that digests basement membrane collagen and the dominant MMP observed on zymography ) and a specific TIMP , TIMP 2 . ^^^ The effect of ethanol and acetaldehyde on MMP 2 and TIMP 2 secretion was also assessed by this method . ^^^ Ethanol and acetaldehyde induced secretion of both MMP 2 and TIMP 2 by PSCs . ^^^ CONCLUSIONS : Pancreatic stellate cells have the capacity to synthesise a number of matrix metalloproteinases , including MMP 2 , MMP 9 , and MMP 13 and their inhibitors TIMP 1 and TIMP 2 . ^^^ Both ethanol and its metabolite acetaldehyde increase MMP 2 as well as TIMP 2 secretion by PSCs . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
OBJECTIVE : To 1 ) determine serum levels of matrix metalloproteinase 2 ( MMP 2 ) , MMP 9 , tissue inhibitor of metalloproteinase 1 ( TIMP 1 ) , and TIMP 2 in patients with secondary progressive ( SP ) MS ; 2 ) determine the relationship between these serum levels and MRI activity ; and 3 ) evaluate the effect of interferon ( IFN ) therapy on these measures . ^^^ METHODS : Monthly Gd+ brain MRI and measures of serum MMP 2 , MMP 9 , TIMP 1 , and TIMP 2 at 3 month intervals were performed for up to 3 years in 33 patients with SPMS participating in a phase 3 study of IFNbeta 1b . ^^^ The ratio of MMP 9 / TIMP 1 predicted new Gd+ lesion on the concurrent scan ( OR = 2 . 23 , 95 % CI 0 . 99 to 4 . 99 , p = 0 . 052 ) and on the following scan ( OR = 2 . 16 , 95 % CI 1 . 01 to 4 . 63 , p = 0 . 048 ) , whereas levels of MMP 2 / TIMP 2 did not . ^^^ IFNbeta did not influence levels of MMP 2 and TIMP 2 . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
METHODS AND RESULTS : Plasma levels of MMP 2 , MMP 9 , MMP 8 , TIMP 1 , TIMP 2 , TNF alpha , TNFR 1 , and TNFR 2 were measured by enzyme linked immunosorbent assay kits . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Levels of MMP 2 , MMP 9 , TIMP 1 , and TIMP 2 were determined in bronchoalveolar lavage by enzyme linked immunosorbent assay . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
We found that EMMPRIN did not regulate MMP 1 , MMP 9 , membrane type 1 MMP ( MT 1 MMP ) expression and had also no effect on the production of the specific tissue inhibitors of MMPs ( TIMPs ) , TIMP 1 and TIMP 2 . ^^^ We also demonstrated that tumor derived EMMPRIN stimulated MMP 1 , 2 , and 3 without modification of MMP 9 , MT 1 MMP , TIMP 1 and TIMP 2 production in human umbilical vein endothelial cells ( HUVEC ) . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
These cells were positive for MMP 2 and MMP 9 as well as TIMP 2 and TIMP 3 . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
When the levels of MMP 1 , MMP 2 , MMP 9 , TIMP 1 , and TIMP 2 were measured , the levels of MMP 2 and TIMP 1 were significantly reduced . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
The levels of interleukin ( IL ) 1alpha , IL 1beta , IL 6 , IL 8 , matrix metalloproteinase ( MMP ) 1 , MMP 2 , MMP 9 , tissue inhibitors of matrix metalloproteinase ( TIMP ) 1 , TIMP 2 , granulocyte elastase , and fetal fibronectin in cervical diluted specimens were measured by immunoassay , and the uterine cervix was assessed by transvaginal ultrasonography . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Imbalance between matrix metalloproteinases ( MMP 9 and MMP 2 ) and tissue inhibitors of metalloproteinases ( TIMP 1 and TIMP 2 ) in acute respiratory distress syndrome patients . ^^^ Concerning the quantity of MMP 2 and the ratio MMP 2 / TIMP 2 , there was no statistical difference between the three groups throughout the study . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
The membrane type 1 matrix metalloproteinase ( MT 1 MMP ) has been identified as a major activator of MMP 2 a process involving the formation of a trimolecular complex with TIMP 2 . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
The ratio of MMP 2 to its principal inhibitor TIMP 2 was balanced in cataract samples , but was decreased in samples from patients with PEXG , resulting in an excess of TIMP 2 over MMP 2 . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
MMP 2 protein in invasive breast cancer and the impact of MMP 2 / TIMP 2 phenotype on overall survival . ^^^ MMP 2 was studied along with clinicopathological parameters ( tumor size , histological type , nuclear and histological grade , stage , lymph node status , ER , and PR ) , patients ' survival and tissue inhibitor metalloproteinase 2 ( TIMP 2 ) , Ki 67 , and p 53 proteins . ^^^ In tumor stromal cells a strong parallel association was observed between the expression of MMP 2 and TIMP 2 ( p = 0 . 015 ) , while an inverse correlation was found between MMP 2 and both Ki 67 and p 53 ( p = 0 . 033 and p = 0 . 034 respectively ) . ^^^ Finally a statistically significant association was revealed using Kaplan Meier and Cox ' s proportional hazard regression model between the MMP 2 / TIMP 2 phenotype and patients ' better survival ( p = 0 . 021 ) . ^^^ Our results point out the strong relation between MMP 2 and TIMP 2 and the effect of the MMP 2 / TIMP 2 phenotype in the patients ' overall survival . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Expression of MMP 1 , MMP 2 , MMP 3 , MMP 9 , MMP 11 , MMP 13 , and MMP 14 and TIMP 1 , TIMP 2 , and TIMP 3 was evaluated by immunohistochemistry on 23 samples of PNC of the skin . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
MAIN OUTCOME MEASURE ( S ) : Activity of MMP 2 and MMP 9 and expression of MMP 1 , TIMP 1 , and TIMP 2 was measured in follicular fluid of the leading follicles by using gel zymography and immunoblot analysis . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
MMP 9 predominates in acute MS lesions and is inhibited by TIMP 1 , while MMP 2 may participate in the remodeling of the extracellular matrix ( ECM ) such as in chronic disease and is inhibited by TIMP 2 . ^^^ We found the MMP 2 / TIMP 2 ratio in serum to show higher values in secondary progressive ( SP , p=0 . 02 ) and primary progressive ( PP , p=0 . 01 ) MS than short disease duration ( SDD ) relapsing remitting ( RR ) MS , but not different from the healthy control ( HC ) group . ^^^ These results show that an increase in MMP 2 / TIMP 2 ratio marks chronic progression in MS , but it is as high as in HC , and also confirm that high MMP 9 activity characterizes short duration relapsing and active forms of the disease . . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Thus the C terminal tail residues of TIMP 2 are important determinants for stable trimolecular complex formation between TIMP 2 , proMMP 2 and MT 1 MMP and play an important role in MT 1 MMP mediated processing to the intermediate and final active forms of MMP 2 at the cell surface . . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Monocytes express MMP 2 , MMP 9 , TIMP 2 , and TIMP 3 . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Formalin fixed paraffin embedded tissue sections from 138 prostatic adenocarcinomas ( PACs ) were immunostained by a combined automated / manual method using monoclonal antibodies against MMP 2 and TIMP 2 . ^^^ One hundred five of 138 ( 76 % ) and 113 / 138 ( 82 % ) PACs expressed MMP 2 and TIMP 2 , respectively . ^^^ In conclusion , MMP 2 and TIMP 2 are co expressed in a majority of PACs and correlate with prognostic variables . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Prognostic significance of the immunoexpression of matrix metalloproteinase MMP 2 and its inhibitor TIMP 2 in laryngeal cancer . ^^^ MATERIAL / METHODS : The immunoexpression of matrix metalloproteinase MMP 2 and its inhibitor TIMP 2 was investigated in 40 laryngeal carcinomas , in order to estimate the prognostic significance of these factors . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Other members of the MMP family ( MMP 9 and MMP 2 ) and the tissue inhibitor of metalloproteinase 2 ( TIMP 2 ) were found to be expressed at similar levels in both populations . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
In normal control eyes retinal ganglion cells expressed MMP 2 , TIMP 1 and TIMP 2 constitutively , and the proteins were detected in their axons . ^^^ At the site of transection , MT 1 MMP , MMP 1 , MMP 2 , TIMP 1 and TIMP 2 were expressed by reactive astrocytes . ^^^ Constitutive expression of MMP 2 , TIMP 1 and TIMP 2 in retinal ganglion cells suggests a role in maintenance of synaptic integrity and plasticity and maintenance of the periaxonal space . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
The gene expression of MMP 1 ( collagenase 1 ) and TIMP 1 , the protein expression of MMP 1 , MMP 2 ( gelatinase A ) , TIMP 1 and TIMP 2 as well as the enzyme activity of MMP 1 and MMP 2 were examined . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
BACKGROUND : The purpose of this study was to quantify the amount of matrix metalloproteinases such as MMP 1 , MMP 2 , MMP 3 , MMP 7 , MMP 9 , and MMP 13 and tissue inhibitors of metalloproteinases TIMP 1 and TIMP 2 expressed by human gingival explants in culture media and the area fraction ( AA % ) of gingival collagen fibers according to the degree of inflammation , to investigate a possible correlation between these enzymes and collagen loss . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
The expression of MMP 9 and MMP 2 and TIMP 1 and TIMP 2 was determined by immunohistochemistry . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Glomeruli were isolated from these and underwent total mRNA extraction followed by RT PCR ELISA to assess transforming growth factor beta 1 , collagen 3 , TIMP 1 , TIMP 2 , and matrix metalloproteinase 2 expression . ^^^ Both had comparable increases in TIMP 1 and matrix metalloproteinase 2 but only rapamycin patients developed a significant increase in TIMP 2 . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
METHODS : We performed gelatin zymography for MMPs and Western blot for MMP 9 , MMP 2 , TIMP 1 , TIMP 2 , TIMP 3 , and TIMP 4 . ^^^ Neither MMP 2 nor TIMP 2 was detected in BAVMs or control brain specimens . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
The aim of this study was to determine whether circulating levels of MMP 2 , MMP 9 , TIMP 1 and TIMP 2 are elevated in the plasma of AD patients . ^^^ Plasma levels of MMP 2 , TIMP 1 and TIMP 2 were unchanged . ^^^ There were no significant changes of MMP 2 , MMP 9 , TIMP 1 and TIMP 2 levels in PD and ALS samples . ^^^ ApoE genotyping of plasma samples showed that levels of MMP 2 , TIMP 1 and TIMP 2 and MMP 9 were not significantly different between the ApoE subgroups . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
METHODS AND RESULTS : MMP 2 , MMP 9 , tissue inhibitor of metalloproteinase ( TIMP ) 1 , and TIMP 2 were immunolocalized in coronary arteries from children with fatal acute KD and controls . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Molecular indicators related to CIA pathology were analysed by measuring the serum levels of matrix metalloproteinase 2 ( MMP 2 ) , tissue inhibitor of matrix metalloproteinase 2 ( TIMP 2 ) and the anti inflammatory cytokines interleukin ( IL ) 4 and IL 10 . ^^^ In the serum of PG 201 treated mice , the level of TIMP 2 and the ratio of TIMP 2 to MMP 2 were significantly elevated , and the level of IL 4 , but not of IL 10 , was increased . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
The 72 kDa protease activity was found to be inhibited by EGTA , 1 : 10 phenanthroline , a 2 macroglobulin and tissue inhibitor of metalloprotease 2 ( TIMP 2 ) indicating that the Ca2+ dependent 72 kDa protease is the MMP 2 . ^^^ Western immunoblot studies of the membrane suspension with polyclonal antibodies of MMP 2 and TIMP 2 revealed that MMP 2 and TIMP 2 , respectively , are the ambient matrix metalloprotease and the corresponding tissue inhibitor of metalloprotease in the membrane . ^^^ Both basal and H2O2 stimulated MMP 2 activity and Ca2+ATPase activity were inhibited by the general inhibitors of matrix metalloproteases : EGTA , 1 : 10 phenanthroline , a 2 macroglobulin and also by TIMP 2 ( the specific inhibitor of MMP 2 ) indicating that H2O2 increased MMP 2 activity and that subsequently stimulated Ca2+ATPase activity in the plasma membrane . ^^^ This was further confirmed by the following observations : ( 1 ) adding low doses of MMP 2 or H2O2 to the smooth muscle membrane suspension caused submaximal increase in Ca2+ATPase activity , and pretreatment with TIMP 2 prevents the increase in Ca2+ATPase activity ; ( 2 ) combined treatment of the membrane with low doses of MMP 2 and H2O2 augments further the Ca2+ATPase activity caused by the respective low doses of either H2O2 or MMP 2 ; and ( 3 ) pretreatment with TIMP 2 prevents the increase in Ca2+ATPase activity in the membrane caused by the combined treatment of MMP 2 and H2O2 . . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Some eyes were homogenized and used to evaluate production of MMP 2 , MMP 9 , TIMP 1 , and TIMP 2 protein , by zymography and reverse zymography . ^^^ Administration of anti IL 1beta just before injury and without bacterial infection resulted in a significant reduction in expression of MMP 2 ( at 8 hours ) , MMP 9 ( at 8 hours ) , TIMP 1 ( at 8 and 72 hours ) , and TIMP 2 ( at 8 hours ) . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
MMP 2 was colocalized with MT 1 MMP and TIMP 2 , which are an activator and an activation enhancing factor , respectively , for proMMP 2 . ^^^ CONCLUSIONS : These data demonstrate that proMMP 2 is efficiently activated in the fibrovascular tissues of PDR , probably through interaction with MT 1 MMP and TIMP 2 , and suggest the possibility that the activity of MMP 2 and MT 1 MMP is involved in the formation of the fibrovascular tissues . . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Fifty five primary and metastatic FIGO stage 3 4 ovarian carcinomas were analyzed for the expression of alpha 5 and beta 1 integrin subunits , the matrix metalloproteinases MMP 2 , MMP 9 , and MT 1 MMP , the MMP inhibitor TIMP 2 , vascular endothelial growth factor ( VEGF ) , basic fibroblast growth factor ( bFGF ) , interleukin 8 ( IL 8 ) , PEA 3 and Ets 1 using mRNA in situ hybridization . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
This suggests an altered MT 1 MMP dependent processing of proMMP 2 into active MMP 2 due to a diminished TIMP 2 level in acute kidney rejection . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
To seek evidence that the nonhuman primate arterial wall , as it ages in the absence of atherosclerosis , exhibits alterations in pathways that are involved in the pathogenesis of experimental atherosclerosis , we assessed aortic matrix metalloproteinase 2 ( MMP 2 ) and its regulators , ie , membrane type 1 of matrix metalloproteinase ( MT 1 MMP ) and tissue inhibitor of matrix metalloproteinase 2 ( TIMP 2 ) , and the expression of angiotensin 2 ( Ang 2 ) , angiotensin converting enzyme ( ACE ) , and chymase in young ( 6 . 4+ / 0 . 7 years ) and old ( 20 . 0+ / 1 . 9 years ) male monkeys . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
An imbalance in MMP 2 / TIMP 2 ratio was noted at P 21 , with elevations occurring in the low and high Dex treated groups . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
The DNA methyltransferase inhibitor 5 azacytidine ( 5 AzaC ) redresses this TIMP 2 / MMP imbalance , reconstituting TIMP 2 expression , without effecting that of MMP 2 , by stimulating TIMP 2 transcription and inhibiting in vitro invasivity of SH SY5Y cells . 5 AzaC stimulated transcription from a nonmethylated TIMP 2 promoter reporter gene construct consistent with regulation of a TIMP 2 transactivator . ^^^ The data highlight a novel indirect methylation mediated mechanism for regulating the TIMP / MMP equilibrium in NB cells , involving repression of TIMP 2 relative to MMP 2 expression , dependent upon suboptimal NF Y transcription factor function , which can be reversed by methyltransferase inhibition . . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Using p47phox / VSMCs , this increase in MMP 2 mRNA was completely blunted . mRNA expression of tissue inhibitor of MMP 2 TIMP 1 and TIMP 2 and membrane type 1 MMP was unaffected by mechanical stretch . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
TIMP 2 ( tissue inhibitor of metalloproteinase 2 ) regulates MMP 2 ( matrix metalloproteinase 2 ) activity in the extracellular environment after pro MMP 2 activation by MT 1 ( membrane type 1 ) MMP . ^^^ A complex of active MT 1 MMP and TIMP 2 binds pro MMP 2 forming a ternary complex , which permits pro MMP 2 activation by a TIMP 2 free neighbouring MT 1 MMP . ^^^ It remains unclear how MMP 2 activity in the pericellular space is regulated in the presence of TIMP 2 . ^^^ To address this question , the effect of TIMP 2 on MMP 2 activity in the extracellular space was investigated in live cells , and their isolated plasma membrane fractions , engineered to control the relative levels of MT 1 MMP and TIMP 2 expression . ^^^ We show that both free and inhibited MMP 2 is detected in the medium , and that the net MMP 2 activity correlates with the level of TIMP 2 expression . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
No induction of MMP 1 , 3 and 7 or variation of the levels of their inhibitors , TIMP 1 and TIMP 2 , were detected . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Each gene studied [ MMP 2 , MMP 3 , MMP 7 , MMP 9 , MMP 12 , MMP 13 , membrane type 1 ( MT 1 ) MMP , TIMP 1 , TIMP 2 , GAPDH , cyclophilin and beta actin ] increased in late pregnancy ( day 21 ) . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
MMPs and TIMPs expressed throughout the cycle ( MMP 2 , MT 1 MMP , MT 2 MMP , MMP 19 , TIMP 1 , and TIMP 2 ) ; 3 ) . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
RNA was isolated from the remaining wounds and skin , and a reduced cycle , primer specific , reverse transcriptase polymerase chain reaction was performed to semiquantitatively determine relative gene expression of MMP 1 , MMP 2 , MMP 7 , MMP 9 , and MMP 14 and of TIMP 1 , TIMP 2 , and TIMP 3 . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
We investigated whether EGCg may affect matrix metalloproteinase 2 ( MMP 2 ) binding to native and denatured type 1 collagen , and binding to the tissue inhibitor of metalloproteinase 2 ( TIMP 2 ) . ^^^ Epigallocatechin 3 gallate binding to MMP 2 inhibits gelatinolytic activity without influencing the attachment to extracellular matrix proteins but enhances MMP 2 binding to TIMP 2 . ^^^ EGCg had no effect on the MMP 2 binding to immobilized native and denatured type 1 collagen , but significantly enhanced pro and activated MMP 2 binding to TIMP 2 , as assessed by immunoprecipitation . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Effects of a vegetable extract from Lupinus albus ( LU 105 ) on the production of matrix metalloproteinases ( MMP 1 , MMP 2 , MMP 9 ) and tissue inhibitor of metalloproteinases ( TIMP 1 , TIMP 2 ) by human gingival fibroblasts in culture . ^^^ Thus LU 105 seems to offer an opportunity to restore a correct balance between MMP 2 , MMP 9 , MMP 1 , and their natural inhibitors , i . e . , TIMP 1 and TIMP 2 in human inflamed gingiva . . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
OBJECTIVE : A novel study has been carried out to characterize the amount and activity levels of metalloproteinases ( i . e . , MMP 1 , MMP 2 , MMP 3 , MMP 8 , MMP 9 and MMP 13 ) and of their inhibitors ( i . e . , TIMP 1 and TIMP 2 ) in synovial fluid from patients ( n = 56 ) with different degrees of either chondral lesions or knee arthritis identified and classified by arthroscopy . ^^^ RESULTS : Analysis of data obtained demonstrates that the degree of cartilage degradation , as seen by arthroscopy , is strictly related to the activity of some synovial MMPs , in particular MMP 2 and MMP 13 and on reduced inhibitory effect of MMP 2 by TIMP 2 ; in addition , a serine protease weighing about 125 kDa appears only in patients with severe cartilage degradation , i . e . , with knee arthritis . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
We used Western blot and immunohistochemistry to determine whether MMP 2 and associated proteins TIMP 2 and membrane type 1 matrix metalloproteinase ( MT 1 MMP ) are present in the olfactory epithelium of mice . ^^^ After injury induced neural degeneration , MMP 2 and MT 1 MMP levels decreased while TIMP 2 levels increased . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Both , TIMP 2 and MMP 2 were increased in the RV only after 14 days ( p < 0 . 05 ) . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
RESULTS : Western blot analysis demonstrated the presence of MMP 1 , MMP 2 , MMP 9 , and MMP 12 , as well as small quantities of tissue inhibitor of metalloproteinases ( TIMP ) 1 and TIMP 2 in protein isolates from normal and varicose veins . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Three laboratory techniques were performed : enzyme linked immunosorbent assays ( MMP 1 , MMP 3 , TIMP 1 , TIMP 2 ) , gelatinase activity assays ( MMP 2 , MMP 9 ) , and quenched fluorescent substrate hydrolysis ( total MMP activity ) . ^^^ Significant negative ( MMP 9 , MMP 3 , MMP 8 , TIMP 2 , total MMP activity ) and positive ( MMP 2 , TIMP 1 ) correlations were observed with the postoperative day , e . g . , total MMP 9 : day 1 , median , 121 ( range , 12 189 ) ng / ml ; day 3 , 46 ( 8 179 ) ; day 5 , 31 ( 0 155 ) , day 7 , 20 ( 6 58 ) . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Decorin infection resulted in decreased protein levels of MMP 1 and MMP 3 whereas MMP 2 and TIMP 2 secretion was increased . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
MMP 2 , MMP 9 , MMP 8 , TIMP 1 and TIMP 2 were detected by immunohistochemistry and the Western blot technique . ^^^ At day 14 p . i . , MMP 2 , 8 and 9 activities were upregulated , and TIMP 2 was expressed . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Finally , we observed a decrease of MMP 1 , MMP 3 , MMP 9 , and MT 1 MMP , both at the mRNA and at the protein levels , in E cadherin positive clones whereas no changes in MMP 2 , TIMP 1 , or TIMP 2 were observed when compared with control clones . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Serum levels of IL 6 , interstitial collagenase ( MMP 1 ) , stromelysin 1 ( MMP 3 ) , gelatinase B ( MMP 9 ) , TIMP 1 and TIMP 2 were assessed with an ELISA technique in 30 RA patients . ^^^ We observed the association between IL 6 and MMP 1 ( p < 0 . 001 ) , MMP 3 ( p < 0 . 05 ) , MMP 9 ( p < 0 . 001 ) , TIMP 1 ( p < 0 . 01 ) and TIMP 2 ( p < 0 . 05 ) . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Organ cultures were performed using explants from the skin of three patients from the same family with recessive dystrophic epidermolysis bullosa to reveal and quantify the expression of MMP 1 ( collagenase 1 ) , MMP 2 and MMP 9 ( gelatinases A and B ) , MMP 3 ( stromelysin 1 ) , TIMP 1 , and TIMP 2 , and to compare the results with those obtained with two human control skins , with the same experimental conditions . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
The invasive potential of Ad p 16 transduced cells was evaluated using a Matrigel invasion assay , and trimolecular complex ( MMP 2 / MT1 MMP / TIMP 2 ) synthesis was proven by zymography and Western blotting . ^^^ However , we were unable to demonstrate any differences in the constitutive productions and secretions of MMP 2 , MT 1 MMP , and TIMP 2 , among the mock treated , Ad lacZ transduced , and Ad p 16 transduced cells . p 16 expression caused an enlargement of all cells , and these were morphologically similar to senescent cells . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
MMP 2 and MT 1 MMP , associated with tissue inhibitor of metalloprotease 2 ( TIMP 2 ) and integrin ( alpha ( 5 ) beta ( 3 ) ) or CD ( 44 ) , is mainly involved in such invasive program . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Hematoxylin and eosin staining , safranin O staining , and immunostaining [ matrix metalloprotease 1 ( MMP 1 ) and tissue inhibitor of metalloprotease 2 ( TIMP 2 ) ] were used for the histological evaluations of transplanted cartilage . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Pro MMP 9 activation by the MT 1 MMP / MMP 2 axis and MMP 3 : role of TIMP 2 and plasma membranes . ^^^ Since pro MMP 2 is activated on the cell surface by MT 1 MMP in a process that requires TIMP 2 , we investigated the role of the MT 1 MMP / MMP 2 axis and TIMPs in mediating pro MMP 9 activation . ^^^ We show that TIMP 2 by regulating pro MMP 2 activation can also act as a positive regulator of pro MMP 9 activation . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
In this study , paraffin embedded material from 84 human pituitary adenomas ( acromegaly n=18 , Cushing ' s disease n=21 , prolactinoma n=18 , thyroid stimulating hormone secreting adenoma n=1 , nonsecreting adenoma n=26 ) and 9 nontumourous anterior pituitary lobes ( obtained from patients with prostate cancer ) was immunohistochemically analysed for expression of MMP 2 , MMP 9 , tissue inhibitor of metalloproteinases 2 ( TIMP 2 ) , urokinase type plasminogen activator ( uPA ) , uPA receptor ( uPAR ) , tissue type plasminogen activator ( tPA ) , plasminogen activator inhibitor 1 ( PAI 1 ) , and interleukin 6 ( IL 6 ) . ^^^ In pituitary adenomas , reactions were positive ( diffuse expression ) to MMP 2 ( 74 % of cases ) , MMP 9 ( 49 % ) , TIMP 2 ( 88 % ) , uPA ( 89 % ) , uPAR ( 90 % ) , tPA ( 69 % ) , and PAI 1 ( 87 % ) . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
We used quantitative RT PCR , Western blot , gelatin zymography and immunohistochemistry to study the expression of VEGF , VEGFR 1 , VEGFR 2 , sVEGFR 1 , neuropilin 1 , MT 1 MMP , MMP 2 , MMP 9 and TIMP 2 in 20 human GBMs and 5 normal brains . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
In this study of 16 subjects with CJD and 16 age , and sex matched controls , we determined the presence of MMP 2 and MMP 9 in their active and proenzyme forms , the relative levels of MMP 3 and four inhibitors of MMP activity ( TIMP 1 , TIMP 2 , TIMP 3 and TIMP 4 ) , and the concentration of 4 3 3 protein . ^^^ The results indicate that , compared with controls , CJD patients have a significantly higher positive frequency of pro MMP 9 and of the active form of MMP 2 , along with significantly higher levels of TIMP 1 and TIMP 2 , classical inhibitors of MMP 9 and MMP 2 , respectively . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
OBJECTIVE : To investigate the expression and significance of transforming growth factor beta ( 1 ) ( TGF beta ( 1 ) ) , matrix metalloproteinase 2 ( MMP 2 ) and tissue inhibitor of metalloproteinase 2 ( TIMP 2 ) in human Lens epithelial cells ( LEC ) of diabetic cataract . ^^^ METHODS : Immunohistochemical staining was used to detect the expression of TGF beta ( 1 ) , MMP 2 and TIMP 2 in the diabetic LEC ( 23 cases ) and the normal LEC ( 7 cases ) . ^^^ CONCLUSION : The imbalance induced by TGF beta ( 1 ) between the expression of MMP 2 and TIMP 2 may play a critical role in the pathological fibrosis of anterior and posterior subcapsular during the development of diabetic cataract . . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Results show that troglitazone , but not pioglitazone and rosiglitazone , stimulated MMP 1 secretion and mRNA expression in both human umbilical vein and aortic endothelial cells , but had no effect on TIMP 1 and TIMP 2 secretion . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Immunostaining for TGF beta , PDGF , TPA , PAI 1 , MMP 2 and TIMP 2 was observed in all allografts at the time of rejection . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
We measured the levels of the vascular endothelial growth factor ( VEGF ) , matrix metalloproteinases type 2 and type 9 ( MMP 2 and MMP 9 ) and tissue inhibitors of matrix metalloproteinase 1 and 2 ( TIMP 1 and TIMP 2 ) in the plasma of patients with ovarian carcinoma ( n=40 ) , in other gynaecological pathologies ( n=30 ) and in the plasma of healthy volunteers ( n=26 ) . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
METHODS : We serially measured the serum levels of MMP 2 , MMP 3 , MMP 9 , MMP 13 , TIMP 1 , TIMP 2 , and laminin in 50 patients with acute ischemic stroke using zymography or enzyme linked immunosorbent assay . ^^^ RESULTS : MMP 2 and MMP 9 increased during the course of ischemia , whereas intact laminin and TIMP 2 decreased significantly ( P < 0 . 05 ) . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Serum concentrations of TNF alpha , interstitial collagenase ( MMP 1 ) , stromelysin 1 ( MMP 3 ) , gelatinase B ( MMP 9 ) , TIMP 1 and TIMP 2 were measured by ELISA in 34 patients with RA . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Expression and clinical significance of MMP 2 , MMP 9 , TIMP 1 , and TIMP 2 in breast carcinoma ] . ^^^ The aim of this study was to investigate the expression of MMP 2 , MMP 9 , TIMP 1 , and TIMP 2 in breast carcinomas and to seek their relationship with breast cancer invasion , metastasis , and prognosis . ^^^ The mRNA expression of TIMP 2 , MMP 2 were determined using in situ hybridization . ^^^ The protein expression of MMP 2 , MMP 9 , TIMP 1 , and TIMP 2 were determined using immunohistochemistry . ^^^ RESULTS : The positive expression rates of TIMP 2 mRNA , MMP 2 mRNA and MMP 2 , MMP 9 , TIMP 1 , and TIMP 2 protein were 66 . 7 % ( 44 / 66 ) , 65 . 2 % ( 43 / 66 ) and 71 . 2 % ( 47 / 66 ) , 68 . 2 % ( 45 / 66 ) , 40 . 9 % ( 27 / 66 ) , 69 . 7 % ( 46 / 66 ) , respectively . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Type 1 collagen induced MMP 2 activation coincides with up regulation of membrane type 1 matrix metalloproteinase and TIMP 2 in cardiac fibroblasts . ^^^ The collagen induced MMP 2 activation coincided with up regulated cellular levels of both membrane type 1 matrix metalloproteinase ( MT 1 MMP ) and TIMP 2 . ^^^ A fraction of cellular membrane prepared from cells embedded in the collagen lattice containing active MT 1 MMP and TIMP 2 was capable of activating pro MMP 2 , and exogenous TIMP 2 had a biphasic effect on this membrane mediated MMP 2 activation . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
In the present study , we assessed the differences in the levels of MMP 2 , MMP 9 , TIMP 1 and TIMP 2 in the normal ovary and ovarian tumours of different histology , including clear cell carcinoma , using specific enzyme linked immunosorbent assays . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
To assess proteolytic cascade imbalance in malignancy progression , the enzymatic activity of MMP 2 and MMP 9 and the expression and serum level of their inhibitors , TIMP 2 and TIMP 1 respectively , was evaluated in selected patients with high risk soft tissue sarcoma ( STS ) . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
We hypothesized that LH or progesterone ( P ) regulate interstitial collagenase ( MMP 1 ) , the gelatinases ( MMP 2 and 9 ) , TIMP 1 , and TIMP 2 in the CL . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Immunohistochemical localization of MMP 2 , MMP 9 , TIMP 1 , and TIMP 2 in the forming rat incisor . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
In this study , serum concentrations of MMP 1 , MMP 2 , MMP 9 , TIMP 1 , and TIMP 2 were determined in 22 patients ( five women and 17 men ; average age , 67 + / 11 years ) with Dupuytren ' s disease using an enzyme linked immunosorbent assay . ^^^ There were no significant differences in the TIMP 2 , MMP 1 , MMP 2 , and MMP 9 serum concentrations between patients with palmar fibromatosis and the control group . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
MAIN OUTCOME MEASURE ( S ) : The expression of MMP 1 , MMP 2 , MMP 9 , TIMP 1 , TIMP 2 , and TIMP 3 in preimplantation embryos . ^^^ The MMP 1 , MMP 9 , and TIMP 2 mRNAs were detected in only a minority of human embryos studied . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Tissue inhibitor of metalloproteinases ( TIMP ) 2 was also elevated in monocytes from multiple sclerosis patients , providing a mechanism for the reported activation of MMP 2 by MMP 14 and TIMP 2 . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
The MMP 2 inhibitory activity of TIMP 1 was clearly elevated 1 and 3 days after exercise , and the MMP 2 inhibitory activity of TIMP 2 rose 1 day after loading . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
The effect of IL 4 on metalloproteinase 2 ( MMP 2 ) and tissue inhibitor of metalloproteinase 2 ( TIMP 2 ) production and gene expression was evaluated . ^^^ IL 4 decreased MMP 2 protein and mRNA levels , and did not alter TIMP 2 production . ^^^ CONCLUSIONS : IL 4 positively regulates procollagen 1 ( alpha 1 ) transcription by direct promoter activation and increases the TIMP 2 / MMP 2 ratio , thereby supporting the profibrotic effect of this cytokine . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Collectively , our studies indicate that elevated levels of TIMP 2 modulate decreased angiogenesis in aged tissues , most likely via TIMP 2 mediated inhibition of MMP 2 and MT 1 MMP . . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Zymographic analysis showed that the levels of MMP 2 and TIMP 2 in melanoma cells were not altered in non contact cocultures when compared with those in individual cultures . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Recent studies have strongly indicated the presence of a functional complex consisting of alpha ( 5 ) beta 3 integrin , membrane type 1 metalloproteinase 2 ( MT 1 MMP ) , and tissue inhibitor of metalloproteinase 2 ( TIMP 2 ) on tumor cell surface , which helps the activation of MMP 2 . ^^^ The association of MMP 2 with alpha ( 5 ) beta 3 integrin and the expression of membrane type MT 1 MMP strongly indicate the role of alpha ( 5 ) beta3 / MT1 MMP / TIMP 2 complex in the activation of MMP 2 in cervical cancer tissue membrane fraction . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
This study compared the expression of TGF beta , collagen , fibronectin , metalloproteinases ( MMP 2 , 9 ) , tissue inhibitors of metalloproteinases ( TIMP 2 ) and osteopontin in renal biopsies obtained from renal transplant recipients treated with either CsA or Tac as primary immunosuppressive agents . ^^^ METHODS : Using RT PCR , intrarenal expression of TGF beta , collagen , fibronectin , MMP 2 , MMP 9 and TIMP 2 were studied in renal biopsies from patients with histological diagnosis of CsA or Tac nephrotoxicity and acute rejection . ^^^ RESULTS : Intrarenal expression of TGF beta , collagen , fibronectin , MMP 2 , TIMP 2 , and osteopontin were significantly increased in patients treated with Tac nephrotoxicity compared with CsA nephrotoxicity . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Selective involvement of TIMP 2 in the second activational cleavage of pro MMP 2 : refinement of the pro MMP 2 activation mechanism . ^^^ A tissue inhibitor of metalloproteinases 2 ( TIMP 2 ) independent mechanism for generating the first activational cleavage of pro matrix metalloproteinase 2 ( MMP 2 ) was identified in membrane type 1 MMP ( MT 1 MMP ) transfected MCF 7 cells and confirmed in TIMP 2 deficient fibroblasts . ^^^ In contrast , the second MMP 2 activational step was found to be TIMP 2 dependent in both systems . ^^^ We propose that the intermediate species of MMP 2 forms the well established trimolecular complex ( MT 1 MMP / TIMP 2 / MMP 2 ) for further TIMP 2 dependent autocatalytic cleavage to the fully active species . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Three micrometer thick , formalin fixed , paraffin embedded tissue sections were immunostained using specific antibodies against MMP 2 , MMP 9 , TIMP 1 , and TIMP 2 . ^^^ RESULTS : MMP 2 expression was detected in 30 tumors ( 71 % ) , and TIMP 2 expression was detected in 31 tumors ( 74 % ) . ^^^ MMP 2 and TIMP 2 expression levels were very low ( 10 % and 0 % , respectively ) in noninvasive tumors but were very high ( 91 % and 97 % , respectively ) in invasive tumors . ^^^ In thymic epithelial tumors , the more progressive the clinical stage of tumor , the higher the strongly positive rate of MMP 2 and TIMP 2 expression . ^^^ Twenty five percent of Type AB thymomas and 50 % of Type B 1 thymomas expressed MMP 2 and TIMP 2 . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
MRL healing is associated with the upregulation of the metalloproteinases MMP 2 and MMP 9 and the downregulation of their inhibitors TIMP 2 and TIMP 3 , both present in inflammatory cells such as neutrophils and macrophages . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
MMP 2 and TIMP 2 expression were not altered by TGF beta 1 . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
In particular , TIMP 2 is involved in a functional complex with the membrane type 1 ( MT 1 ) MMP to convert the secreted MMP 2 progelatinase into the fully active proteolytic enzyme . ^^^ We used the human , androgen sensitive prostate carcinoma cell line LNCaP in coculture with the human osteosarcoma cell line OHS to experimentally address the possibility of androgen dependent regulatory effects on the functional MT 1 MMP / TIMP 2 / MMP 2 complex upon interaction between prostate carcinoma and osteoblastic cells in metastasis of prostate cancer to bone . ^^^ However , R 1881 treatment of the LNCaP / OHS coculture model did not seem to change the overall proteolytic activity of the MT 1 MMP / TIMP 2 / MMP 2 complex . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Single nucleotide polymorphisms identified in the promoters of MMP 2 ( 1306C > T ) and TIMP 2 ( 418G > C ) abolish the Sp 1 binding site and thus may down regulate expression of the genes . ^^^ MMP 2 genotypes were determined by PCR based denaturing high performance liquid chromatography analysis and TIMP 2 genotypes identified by a PCR RFLP method in 462 breast cancer patients and 509 frequency matched control women . ^^^ These findings suggest that the presence of the variant allele in the promoter of MMP 2 or TIMP 2 may be a protective factor for the development of breast cancer . . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Additionally , the effect of tissue inhibitor of MMP 1 ( TIMP 1 ) and TIMP 2 on SAS cell growth was investigated . ^^^ Dose dependent inhibitory effects of TIMP 2 , but not TIMP 1 , against MMP 2 activation were observed in SAS . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
METHODS : Immunoblot and zymography were used to detect the presence or absence of MMP 1 and 7 , tissue inhibitor of metalloproteinase 1 and 2 ( TIMP 1 , TIMP 2 ) , plasminogen activator activity and plasminogen activator inhibitor 1 ( PAI 1 ) . ^^^ RESULTS : We detected MMP 7 , urokinase plasminogen activator ( uPA ) and PAI 1 in both Matrigels , TIMP 2 was detected only in regular Matrigel and no MMP 1 or TIMP 1 was detected in either matrix . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Quantitative PCR measurements of MMP 2 and MMP 9 , TIMP 1 and TIMP 2 , and MxA were performed in peripheral mononuclear cells from clinically stable multiple sclerosis patients with relapsing remitting disease course after short term and long term treatment with IFN beta . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
We have studied the expression of MMP 2 , MMP 9 tissue inhibitor of metalloprotein 1 ( TIMP 1 ) , TIMP 2 and membrane type 1 MMP ( MT 1 MMP ) in the wall of small AAAs ( 30 45 mm ) , large AAAs ( > 45 mm ) and controls ( < 25 mm ) . ^^^ We investigated the relationship among expressions of MMP 2 , TIMP 2 and MT 1 MMP in the walls . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
We demonstrate for the first time in vascular endothelial cells : ( 1 ) the involvement of signaling intermediates in the PAF PAF receptor system in the induction of TIMP 2 and MT 1 MMP expression , resulting in the coordinated proteolytic activation of MMP 2 , and ( 2 ) a receptor mediated signal transduction cascade for the tyrosine phosphorylation of FAK by PAF . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Moreover , IL 18 could significantly increase MMP 9 but not MMP 2 production at both mRNA and / or protein level , slightly up regulate TIMP 1 mRNA , and clearly induce TIMP 2 mRNA secretion . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Exogenous addition of comparable levels of purified TIMP 2 to parental U87MG cells increased MMP 2 activation and invasion . ^^^ Similarly , U87MG cells engineered to overexpress TIMP 2 at the same levels as U 87 C1 cells also demonstrated increased MMP 2 activation , indicating that an increase in physiological levels of TIMP 2 can promote MMP 2 activation and invasion in glioblastoma cells . ^^^ However , exogenous administration or recombinant overexpression of higher amounts of TIMP 2 in U87MG cells resulted in inhibition of MMP 2 activation . ^^^ These results demonstrate that the complex balance between TIMP 2 and MMP 2 is a critical determinant of glioblastoma invasion , and indicate that increasing TIMP 2 in glioblastoma patients may potentially cause adverse effects , particularly in tumors containing high levels of MT 1 MMP and MMP 2 . . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
In this study we examined the expression by gingival mast cells of matrix metalloproteinases , MMP 1 , MMP 2 , MMP 8 and the tissue inhibitors of metalloproteinases , TIMP 1 and TIMP 2 . ^^^ METHODS : Gingival specimens from 12 human immunodeficiency virus negative ( HIV ) and 15 HIV positive ( HIV+ ) patients with chronic marginal periodontitis ( CMP ) , and from 10 HIV and four HIV+ controls with clinically healthy gingiva ( HG ) were examined after double immunofluorescence staining for mast cell tryptase , combined with antibodies for MMP 1 , MMP 2 , MMP 8 or their inhibitors TIMP 1 and TIMP 2 . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Both primary and metastatic tumors express the matrix metalloproteinases ( MMP 2 ) , MT 1 MMP ( MMP 14 ) and tissue inhibitor of metalloproteinases ( TIMP 2 ) . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
OBJECTIVE : To explore the expression and clinical prognostic significance of matrix metalloproteinase 2 mRNA ( MMP 2 mRNA ) , tissue inhibitor of matrix metalloproteinase 2 mRNA ( TIMP 2 mRNA ) , matrix metalloproteinase 2 protein ( MMP 2 ) , matrix metalloproteinase 9 protein ( MMP 9 ) , tissue inhibitor of matrix metalloproteinase 1 protein ( TIMP 1 ) , and tissue inhibitor of matrix metalloproteinase 2 protein ( TIMP 2 ) in the hepatocellular carcinomas ( HCCs ) . ^^^ METHODS : Fifty six specimens of HCCs from 56 patients , who were followed up , were investigated by in situ hybridization with specific probes for MMP 2 , TIMP 2 , and by immunohistochemistry with anti MMP 2 , MMP 9 and anti TIMP 1 , TIMP 2 monoclonal antibody . ^^^ The positive expression of MMP 2mRNA , TIMP 2mRNA , MMP 2 protein , MMP 9 protein , TIMP 1 protein and TIMP 2 protein in the 56 HCCs cases were 48 ( 85 . 7 % ) , 35 ( 62 . 5 % ) , 44 ( 78 . 6 % ) , 41 ( 73 . 2 % ) , 30 ( 53 . 6 % ) , and 38 ( 68 % ) , respectively . 2 . ^^^ There was a positive association between TIMP 2mRNA and TIMP 2 protein expression , and between MMP 2 mRNA and MMP 2 protein in HCCs , respectively ( r = 0 . 316 , P < 0 . 05 ; r = 0 . 356 , P < 0 . 05 ) . 4 . ^^^ These results were further confirmed by multivariate analysis in which MMP 2 protein and MMP 9 protein emerged as independent prognostic factors for poor survival regardless of the age , tumor size , tumor grades , TNM classification and expression of MMP 2mRNA , TIMP 2mRNA , TIMP 1 protein and TIMP 2 protein . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
In this study , we have analyzed the expression of two matrix metalloproteinases ( MMPs ) : MMP 2 ( 72 kDa ) and MMP 9 ( 92 kDa ) and their specific tissue inhibitors TIMP 1 and TIMP 2 in primary human osteoblastic cells . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
In an attempt to investigate potential markers for bone marrow remodelling processes , we investigated plasma levels of total , free and complexed TIMP 1 , TIMP 2 , MMP 2 and MMP 9 in a patient cohort comprising 17 with myelofibrosis ( MF ) , 17 with polycythaemia vera ( PV ) , 15 with essential thrombocythaemia ( ET ) , 1 with a transitional MPD and 30 controls . ^^^ No statistical significant difference between TIMP 2 and MMP 2 levels was observed between patients and controls . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
The inhibitor was also found to be sensitive to the activated 72 kDa gelatinase TIMP 2 complex and also active human interstitial collagenase . ^^^ Bovine pulmonary artery smooth muscle possesses the tissue inhibitor of matrix metalloproteinase 2 ( TIMP 2 ) as revealed by Western immunoblot study of its cytosol fraction with bovine polyclonal TIMP 2 antibody . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Remarkably , active MMP 2 was restricted to this cell line , whereas TIMP 2 and membrane type ( MT ) 1 MMP expression was comparable in all three of the spontaneously metastasizing melanoma cell lines investigated . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
METHODS : MMP 2 and MMP 9 were analyzed in 35 cholangiocarcinomas and 4 normal livers with fresh tissue using zymography and real time reverse transcription polymerase chain reaction ( RT PCR ) , and expressions of MMP 2 , MMP 9 , TIMP 1 , and TIMP 2 were evaluated in 76 cholangiocarcinomas by immunohistochemistry . ^^^ The balanced expressions of MMP 2 / TIMP 2 and MMP 9 / TIMP 1 were significantly decreased in the MF and PI types , compared to the IG type . ^^^ CONCLUSIONS : The activities of MMP 2 and loss of balanced expressions of MMP 2 / TIMP 2 and MMP 9 / TIMP 1 are suggested as playing important roles in invasive growth related to the gross type of cholangiocarcinoma . . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
The protein expression level of secreted MMP 2 , MMP 9 , TIMP 1 , TIMP 2 and intracellular expression level of p 53 , p 16 , PCNA , CD 44 ( V 6 ) isomeride , E cadherin , CK 18 , nm 23 H1 as well as the mRNA expression level of MMP 2 , MMP 9 , TIMP 1 , TIMP 2 , VEGF were compared through Western blot . ^^^ Semi quantitative RT PCR analysis was used to determine the intracellular mRNA expression of MMP 2 , MMP 9 , TIMP 1 , TIMP 2 and VEGF . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
To investigate the roles of matrix metalloproteinases ( MMPs ) in thymic epithelial tumors , we examined the expression of MMP 2 , 7 , and 9 ; membrane type 1 ( MT 1 ) MMP ; and tissue inhibitor of metalloproteinase 2 ( TIMP 2 ) in 57 tumors by immunohistochemistry and in selected 15 cases by in situ hybridization . ^^^ Expression of MT 1 MMP and TIMP 2 correlated with that of MMP 2 , indicating a proteolytic activation of the latter . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Analogous to the complex of the hemopexin like domain of gelatinase A and TIMP 2 ( Olson , M . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
RESULTS : The resected lateral rectus muscle had significantly smaller amounts of TIMP 1 , TIMP 2 and BMP 4 but larger amounts of MMP 2 compared to the medial rectus muscle ( P < 0 . 001 , Mann Whitney U test ) . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Isolated cell membranes from cell lines with a reduced S100A4 level contained less MT 1 MMP , MMP 2 and TIMP 2 compared to the control cells . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Matrix metalloproteinase 2 ( MMP 2 ) , which degrades Type 4 collagen , is produced as proMMP 2 . proMMP 2 is activated in part through its interactions with membrane Type 1 MMP ( MT 1 MMP ) and tissue inhibitor of matrix metalloproteinase 2 ( TIMP 2 ) . ^^^ Matrix metalloproteinase 2 ( MMP 2 ) , which degrades Type 4 collagen , is produced as proMMP 2 . proMMP 2 is activated in part through its interactions with membrane Type 1 MMP ( MT 1 MMP ) and tissue inhibitor of matrix metalloproteinase 2 ( TIMP 2 ) . ^^^ We show that PAF increases mRNA levels for MT 1 MMP and TIMP 2 , followed by increased temporal conversion of latent proMMP 2 to MMP 2 . ^^^ Finally , we demonstrate that the ratio of MT 1 MMP to TIMP 2 in membrane preparations from PAF stimulated HUVEC is 1 . 6 : 1 , approximating the hypothesized ideal ratio of 2 : 1 necessary for the conversion of proMMP 2 to MMP 2 . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
METHODS : Using growth inhibition , Zymograms assays and reverse transcription polymerase chain reaction ( RT PCR ) , we examined cell growth rates , activities of matrix metalloproteinases 2 ( MMP 2 ) and 9 ( MMP 9 ) , and expression of metalloproteinases 1 ( TIMP 1 ) and 2 ( TIMP 2 ) in SGC 7901 cells after the treatment with beta ionone for 24 h and 48 h , respectively . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Binding of tissue inhibitor of metalloproteinase 2 ( TIMP 2 ) to pro MMP 2 and mature membrane type 1 MMP ( MT 1 MMP ) on the cell surface is required for activation of MMP 2 . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
LA CVF 1 significantly correlated with LA dimension and TIMP 2 to MMP 2 ratio . ^^^ CONCLUSIONS : Atrial extracellular matrix remodeling manifested by the selective downregulation of TIMP 2 along with upregulation of MMP 2 and CVF 1 in the atrium is associated with the development of sustained atrial fibrillation in patients with cardiomyopathy and heart failure . . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Furthermore , both significant suppression of matrix metalloproteinases ( MMPs ) activity such as MMP 2 and significant activation of tissue inhibitors of MMP ( TIMPs ) such as TIMP 2 observed in the 5 / 6Nx rats were almost completely prevented by fluvastatin , resulting in a significant prevention of glomerular ECM accumulation . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
MMP 1 , 3 , and 9 and TIMP 2 were either not detected or their secretion was not consistently influenced by selenium treatment . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
MMP 2 , MMP 9 and their inhibitors TIMP 2 and TIMP 1 production by human monocytes in vitro in the presence of different forms of hydroxyapatite particles . ^^^ In the present work , we have evaluated the effect of HA particles characteristics ( size , shape and sintering temperature ) on the MMP 2 , 9 and their respective inhibitors TIMP 2 , 1 production . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
The expression of MMP 2 , tissue inhibitor of metalloproteinase 2 ( TIMP 2 ) , and membrane type MMP 1 ( MT 1 MMP ) in PC 3 parental and transfected cells under serum free conditions was determined by zymography , immunoblotting , immunofluorescent microscopy , Northern blotting , and / or reverse transcriptase polymerase chain reaction ( RT PCR ) . ^^^ The molecular basis for the low level of processing of pro MMP 2 by PC 3 cells may be due to an overabundance of TIMP 2 and / or a low level of cell surface active MT 1 MMP . . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Membrane type 1 MMP ( MT 1 MMP ) and tissue inhibitor of metalloproteinase 2 ( TIMP 2 ) are essential factors for the activation of pro MMP 2 . ^^^ Expressions of MMP 2 , MMP 9 , MT 1 MMP , and TIMP 2 were examined using immunohistological methods on the sections of tumor biopsy samples . ^^^ RESULTS : The expression score of MMP 2 correlated with that of MMP 9 ( r = 0 . 291 ; P = 0 . 036 ) , MT 1 MMP ( r = 0 . 286 ; P = 0 . 039 ) , and TIMP 2 ( r = 0 . 257 ; P = 0 . 050 ) . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Serum concentrations of interstitial collagenase ( MMP 1 ) , stromelysin 1 ( MMP 3 ) , gelatinase B ( MMP 9 ) , TIMP 1 , and TIMP 2 were measured by ELISA prior to infusion at Weeks 0 , 2 , 6 , 14 , 38 , and 62 . ^^^ RESULTS : The initial infusion of infliximab downregulated serum levels of MMP 1 ( p < 0 . 001 ) , MMP 3 ( p < 0 . 001 ) , MMP 9 ( p < 0 . 001 ) , TIMP 1 ( p < 0 . 01 ) , and TIMP 2 ( p < 0 . 05 ) . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
OBJECTIVE : To investigate matrix metalloproteinase 2 ( MMP 2 ) and tissue inhibitor of metalloproteinase 2 ( TIMP 2 ) expressions in the cultured rat mesangial cells ( MsC ) transfected with Smad 7 vector and to elucidate the mechanism of Smad 7 in blocking tissue fibrosis . ^^^ The expressions of MMP 2 and TIMP 2 were determined by Western blot , RT PCR and zymography assay . ^^^ CONCLUSIONS : It is possible that Smad 7 can alleviate the development of tissue fibrosis by upregulating the expression of MMP 2 and downregulating the expression of TIMP 2 in mesangial cells . . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
OBJECTIVE : To explore the effect of transforming growth factor ( TGF ) beta1 / Smad signaling pathway on the expression and enzymatic activity of matrix metalloproteinase 2 ( MMP 2 ) and tissue inhibitor of metalloproteinase 2 ( TIMP 2 ) in cultured rat mesangial cells ( MsC ) . ^^^ The expression and enzymatic activity of MMP 2 and TIMP 2 were determined by Western blot , zymography or reverse zymography assay . ^^^ RESULTS : MsC transfected with Smad 2 gene showed slightly increased expression and enzymatic activity of both MMP 2 and TIMP 2 , which was more obvious upon stimulation by TGF beta 1 . ^^^ On the other hand , MsC transfected with Smad 7 gene showed a decrease in MMP 2 and TIMP 2 expression and enzymatic activity , which was especially obvious after stimulation by TGF beta 1 . ^^^ CONCLUSIONS : TGF beta1 / Smad signaling pathway may play an important role in the pathogenesis of glomerulosclerosis , probably via MMP 2 and TIMP 2 expression and the associated enzymatic activity . . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
OBJECTIVE : To observe the changes in matrix metalloproteinase 2 , 7 ( MMP 2 , 7 ) and tissue inhibitor of metalloproteinase 2 ( TIMP 2 ) in deep partial thickness burn during the process of wound healing , and the effects of bFGF on wound healing . ^^^ Changes in MMP 2 , 7 and TIMP 2 expressions were an important process of wound repair , which was closely related to the acceleration of wound healing by the application of bFGF . . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
AhR / MEF had lower levels of matrix metalloproteinase 2 ( MMP 2 ) activity , which could not be attributable to MMP 2 mRNA downregulation or MMP inhibitors Timp 1 and Timp 2 overexpression . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
METHODS : MMP 2 , MMP 2 activator , MT 1 MMP , and its inhibitor ( TIMP 2 ) were immunostained in 84 primary epithelial ovarian carcinomas ( EOCs ) ( 35 endometrioid adenocarcinomas [ ECs ] and 49 serous adenocarcinomas [ SCs ] ) . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Regulation of the balance of matrix metalloproteinase 2 ( MMP 2 ) and its tissue inhibitor ( TIMP 2 ) by thioredoxin ( Trx ) was investigated in human dermal fibroblasts . ^^^ Incubation with recombinant Trx or TR decreased TIMP 2 activity and increased MMP 2 activity . ^^^ In conclusion , thioredoxin secreted by UVA irradiation is involved in the regulation of MMP 2 and TIMP 2 activities through its redox activity in human dermal fibroblasts . . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Human tricuspid aortic valves with and without calcific aortic stenosis were studied by immunohistochemistry for MMP 2 , MMP 9 and TIMP 2 . ^^^ Staining intensities for MMP 2 and TIMP 2 were elevated in stenotic valves as compared to controls . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
CONCLUSIONS AND CLINICAL RELEVANCE : Healthy calves have detectable amounts of the gelatinases MMP 2 and 9 in BALF Endogenous inhibitors of MMPs were detected in BALF ( ie , TIMP 1 , all calves ; TIMP 2 , 4 calves ) . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
RT PCR analysis indicated that MMP 2 , membrane type 1 MMP ( MT 1 MMP ) and tissue inhibitor of metalloproteinase 2 ( TIMP 2 ) mRNA levels were elevated in HSCs cultured on type 1 collagen gel . ^^^ These results indicate that the native fibrillar ( polymerized ) but not monomeric form of type 1 collagen induced pro MMP 2 production and activation through MT 1 MMP and TIMP 2 in cultured HSCs , suggesting an important role of HSCs in ECM remodeling in the hepatic perisinusoidal spaces . . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Quantitative reverse transcription PCR was used to determine the expression levels of MMP 1 , MMP 2 , MMP 3 , MMP 7 , MMP 11 , MMP 12 , MMP 13 , MMP 14 and the inhibitors TIMP 1 , TIMP 2 and TIMP 3 . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
The production of MMP 2 , MMP 9 , TIMP 1 and TIMP 2 was assessed by gelatin zymography and enzyme linked immunoabsorbent assay before and after interleukin 1alpha ( IL 1alpha ) and interleukin 6 ( IL 6 ) stimulation ; cell invasiveness was measured by Matrigel invasion assay . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Here we present the first theoretical model of the biochemical network describing the proteolysis of collagen 1 by matrix metalloproteinases 2 ( MMP 2 ) and membrane type 1 matrix metalloproteinases ( MT 1 MMP ) in the presence of the tissue inhibitor of metalloproteinases 2 ( TIMP 2 ) in a bulk , cell free , well stirred environment . ^^^ We show that pro MMP 2 activation , a process that involves a trimer formation of the proenzyme with TIMP 2 and MT 1 MMP , is suppressed at high inhibitor levels and paradoxically attains maximum only at intermediate TIMP 2 concentrations . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Following FTS treatment , the MMP 2 and MMP 9 induced collagenolytic activity and TIMP 2 expression , were increased in FTS compared to PBS treated rats . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Plasma MMP 9 and MMP 2 levels correlated with emboli number ( r=0 . 544 [ p=0 . 003 ] and r=0 . 412 , [ p=0 . 033 ] , respectively ) , while TIMP 1 and TIMP 2 levels did not . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
The assay included mixing of MMP 2 with antibody Ab 2 adsorbed to colloidal gold with a diameter of about 20nm and injection into the flowcell of the SPR instrument containing immobilized TIMP 2 . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
MMP 2 and TIMP 2 increased similarly in all infarcted groups . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
The increase in TGF beta isoforms correlated with that of the mRNA expression of collagens , MMP 2 and TIMP 2 . . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
RT PCR and Western blotting were used to detect the expressions of mRNA and protein of TIMP 1 , TIMP 2 , MMP 2 , and MMP 9 in the kidney at different time points . ^^^ In the control groups , TIMP 1 and TIMP 2 Both MMP 2 mRNA and MMP 9 mRNA were expressed in both control groups , without significant differences between these 2 control groups . ^^^ The MMP 2 activity and MMP 9 activity at different time points of the 2 UUO groups were all significantly lower than those of the control groups of the same age , and those of the 26 month old were significantly lower than those of the 3 month old rats The MMP 2 activity and MMP 9 activity were negatively correlated with the expressions of TIMP 2 and TIMP 1 . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Similar to 25 mM glucose , addition of rhCTGF increased MMP 2 , TIMP 1 , and TIMP 3 mRNA by 2 . 5 , 2 . 1 , and 1 . 6 fold , respectively ( P < 0 . 05 ) but had no effect on membrane type ( MT ) 1 MMP or TIMP 2 . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
In HD patients , with and without cardiovascular disease ( CVD ) , and in controls , we compared pre dialysis levels of MMP 2 , MMP 9 , TIMP 1 , TIMP 2 ; the markers of extrinsic coagulation pathway tissue factor ( TF ) and its inhibitor ( TFPI ) , prothrombin fragment F1+2 ( F1+2 ) ; a marker of SOX Cu / Zn superoxide dismutase ( Cu / Zn SOD ) and a surrogate of atherosclerotic disease intima media thickness ( IMT ) . ^^^ Multiple stepwise regression analysis showed that low TIMP 2 followed by increased Cu / Zn SOD and MMP 2 levels independently and significantly predicted elevated IMT on maintenance HD patients . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
On the contrary , the ratio of TIMP 2 to MMP 2 significantly increased in the IL 4 DNA treated group ( P < 0 . 01 ) . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Tissue levels of extracellular matrix inducer protein ( EMMPRIN ) , MMP activator protein ( MT 1 MMP ) , MMP 1 , MMP 2 , and MMP 9 , as well as tissue inhibitors of MMPs ( TIMP 1 and TIMP 2 ) were assessed by immunoblotting and yielded a significant decrease in MMP 9 , EMMPRIN , and MT 1 MMP levels in hypertension . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Cytokine ( TNF alpha , IL 1beta , IL 6 and IL 10 ) and metalloproteinase ( MMP 2 and MMP 9 ) release as well as their inhibitors ( TIMP 1 and TIMP 2 ) were assessed after time course incubation with different amount of nanoparticles . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
PATIENTS AND METHODS : To assess the proteolytic cascade imbalance in malignancy progression , tissue expression and serum levels of MMP 2 , MMP 9 and of their inhibitors TIMP 2 and TIMP 1 respectively were evaluated in 42 selected patients with high grade osteosarcoma ( OS ) . ^^^ MMP 2 , MMP 9 , TIMP 2 and TIMP 1 were studied in biopsies by immunohistochemistry and in serum by ELISA test . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Pretreatment with superoxide dismutase ( SOD ) and tissue inhibitor of metalloproteinase ( TIMP 2 ) ( 50 microg ml ( 1 ) ) , preserved the increase in MMP 2 activity , Ca2+ ATPase activity and also ATP dependent Ca2+ uptake in the microsomes . ^^^ O2 * induced changes in MMP 2 activity , ATP dependent Ca2+ uptake and Na+ dependent Ca2+ uptake , were not reversed upon pretreatment of the microsomes with a low dose ( 5 microg ml ( 1 ) ) of TIMP 2 which , on the contrary , reversed MMP 2 ( 1 microg ml ( 1 ) ) mediated alteration on these parameters . ^^^ Treatment of TIMP 2 ( 5 microg ml ( 1 ) ) with the O2 * generating system abolished the inhibitory effect of TIMP 2 ( 5 microg ml ( 1 ) ) on MMP 2 ( 1 microg ml ( 1 ) ) ( measured by ( 14 ) C gelatin degradation ) . ^^^ Overall , the present study suggests that O2 * inactivated TIMP 2 , the ambient inhibitor of MMP 2 , leading to activation of the ambient proteinase , MMP 2 , which subsequently stimulated Ca2+ ATPase activity and ATP dependent Ca2+ uptake , but inhibited Na+ dependent Ca2+ uptake , resulting in a marked decrease in Ca2+ uptake in the smooth muscle microsomes . . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
We have previously indicated that bovine pulmonary artery smooth muscle plasma membrane possesses a complex of 72 kDa gelatinase and TIMP 2 ( MMP 2 / TIMP 2 complex ) [ Mol . ^^^ Isolation of MMP 2 from MMP 2 / TIMP 2 complex : characterization of the complex and the free enzyme in pulmonary vascular smooth muscle plasma membrane . ^^^ In this paper , we described isolation of MMP 2 from the MMP 2 / TIMP 2 complex , characterizations of the isolated MMP 2 and also the complex . ^^^ MMP 2 / TIMP 2 complex was purified from bovine pulmonary vascular smooth muscle plasma membrane using a combination of purification steps . ^^^ Heparin sepharose ( 100 mM NaCl eluate ) purified preparation contained the MMP 2 / TIMP 2 complex . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
No change in MMP 2 secretion ( 111 . 6 + / 5 . 2 % ; P > 0 . 05 ) was observed but both TIMP 1 and TIMP 2 expression were increased to 136 . 3 + / 6 . 4 % and 144 . 0 + / 27 . 5 % , respectively ( both P < 0 . 05 ) . ^^^ This was paralleled by increased secretion of MMP 2 to 145 . 8 + / 7 . 8 % ( P < 0 . 01 ) , increased TIMP 2 expression to 208 . 0 + / 21 . 3 % ( P < 0 . 005 compared with high glucose ) but no change in TIMP 1 expression ( 155 . 1 + / 14 . 6 % ) compared with high glucose alone . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
PDGF elevated the levels of MMP 1 or TIMP 1 protein as well as mRNA , whereas this cytokine had no influence on the expression of type 1 collagen , FN , or TIMP 2 . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Expression and localization of matrix metalloproteinases ( MT 1 MMP , MMP 2 ) and tissue inhibitor of metalloproteinase 2 ( TIMP 2 ) during synepitheliochorial placentation of goats ( Capra hircus ) . ^^^ In the present study , expression patterns of MT 1 MMP , MMP 2 and TIMP 2 mRNAs as well as the encoded proteins in the endometrium and the placenta were examined on Days 35 , 75 , and 100 of pregnancy , representing roughly the 1st , 2nd and 3rd trimesters of caprine gestation , by means of quantitative RT PCR analysis , in situ hybridization , immunoblotting , gelatin zymography and immunohistochemistry . ^^^ Curiously , however , in the placentomes , the relative expression levels of MT 1 MMP mRNA increased linearly from Day 35 to Day 100 , while those of MMP 2 and TIMP 2 were clearly down regulated in Day 100 placentae . ^^^ The co localization of MT 1 MMP , MMP 2 and TIMP 2 in binucleate trophoblast cells , the cotyledonal trophoblast cells and the subsyncytial stromal cells is likely to reflect the functional coordination of the 3 proteins in these cells during trophoblastic invasion and the placental tissue remodeling in the placentome . . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Here , we examined the immunohistochemical expression of MMP 2 , MMP 3 , MMP 11 , tissue inhibitor metalloproteinase ( TIMP ) 1 and TIMP 2 in endometrium from women with ( n=9 ) or without endometriosis ( n=18 ) in comparison with peritoneal ( n=20 ) , ovarian ( n=20 ) and colorectal endometriosis ( n=20 ) . ^^^ These results suggest that MMP 2 and 3 and TIMP 2 may be involved in the pathogenesis of endometriosis . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
These findings indicate that the histologic heterogeneity of lung adenocarcinomas is partly related to the cancer cell stromal interaction , in particular through BM preservation or destruction , which was mainly affected by cancer cell expression of MMP 2 and TIMP 2 in lung adenocarcinomas . . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
CONCLUSIONS : MMP 9 , MMP 2 , MMP 7 , TIMP 2 , TIMP 3 are over expressed in ovarian malignant tissues . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
RESULTS : Immunohistochemical staining revealed that MMP 2 and tissue inhibitors of metalloproteinase 2 ( TIMP 2 ) were expressed more strongly in the neointima and media of the control CAV animals than in the ONO CAV animals . ^^^ The animals given ONO 4817 exhibited a significant decrease in the percentage of affected vessels , in the percentage of intimal proliferation , in the intima to media ratio , and in the expression of MMP 2 and TIMP 2 . ^^^ CONCLUSION : These results suggest that MMP 2 and TIMP 2 play an important role in the development of CAV and that the use of an MMP inhibitor ( ONO 4817 ) may prevent neointimal proliferation in patients with CAV . . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
There was no effect on gene expression of collagen 1 , MMP 2 , or TIMP 2 . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
However , the zymogen of MMP 2 ( pro MMP 2 ) mostly occurs in tissues as a complex with the tissue inhibitor of MMPs ( TIMP 2 ) . ^^^ TIMP 2 complex is also mediated by LRP , because addition of receptor associated protein ( RAP ) , a natural LRP ligand antagonist , inhibited endocytosis and lysosomal degradation of ( 125 ) 1 pro MMP 2 . ^^^ Both TIMP 2 and the pro MMP 2 collagen binding domain independently competed for endocytosis of ( 125 ) 1 pro MMP 2 . ^^^ Surface plasmon resonance studies indicated that pro MMP 2 , TIMP 2 , and pro MMP 2 . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Effects of puerarin on renal function , expressions of MMP 2 and TIMP 2 in diabetic rats ] . ^^^ AIM : To investigate the effect of puerarin on expressions of MMP 2 and TIMP 2 in the kidney of diabetic rats . ^^^ Using in situ hybridization and immunohistochemistry to detect MMP 2 , TIMP 2 mRNA expressions and MMP 2 , TIMP 2 , collagen 4 and Laminin expressions in diabetic kidneys with image analysis system , Flow cytometry was used to detect the expressions of TGFbeta 1 , MMP 2 and TIMP 2 . ^^^ RESULTS : Compared with those in kidneys of control group , expressions of MMP 2 mRNA and proteins were lower , while the expressions of both TGFbeta 1 and TIMP 2 were higher in the diabetic kidney ( P < 0 . 05 ) . ^^^ The level of MMP 2 expression was advanced , while expression of TIMP 2 was reduced by puerarin treatment ( P < 0 . 05 ) . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Among the numerous proteolytic enzymes of this group the key role is played by metalloproteinase 2 ( MMP 2 ) and tissue inhibitor of metalloproteinase 2 ( TIMP 2 ) . ^^^ The aim of the study was to evaluate the concentration of MMP 2 and TIMP 2 in blood serum of patients with benign and malignant thyroid tumours and the effect of surgical treatment on these parameters in the postoperative period as well as assessment whether to MMP 2 and TIMP 2 serum concentration in patients with thyroid cancer positively correlates with the clinical staging classification of the International Union Against Cancer ( UICC ) . ^^^ Additionally , a significant effect of radical surgical treatment on mean concentration of MMP 2 and TIMP 2 in patients with papillary and follicular thyroid cancer was demonstrated . ^^^ CONCLUSIONS : MMP 2 and its tissue inhibitor TIMP 2 apparently play a significant role in the pathogenesis of thyroid cancer . ^^^ Serum MMP 2 and TIMP 2 concentrations in patients with thyroid cancer did not significantly correlate with the clinical staging of thyroid cancer . . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Urine was collected preoperatively and for 24 days after surgery and tested for MMP 2 , MMP 9 , TIMP 1 , and TIMP 2 using substrate gel electrophoresis ( zymography ) and Western blot analysis . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Furthermore , the presence of MMP 1 and MMP 2 and their tissue inhibitors TIMP 1 and TIMP 2 was revealed by immunohistochemistry . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
By incubation of MMP 2 TIMP 2 complex with equal molar rhMMP 7 , MMP 2 was dissociated from the complex and activated in a time dependent manner , suggesting that TIMP 2 helps to keep the latency of MMP 2 . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
The aim of the present study was to determine MMP 2 , MMP 9 , TIMP 1 and TIMP 2 protein expression in decidual endometrium during the first trimester of pregnancy ( 22 42 days post coitus ) with special attention to their expression patterns in endometrial compartments . ^^^ We conclude that ( 1 ) MMP 2 and MMP 9 and their inhibitors TIMP 1 , and TIMP 2 determine the invasive behavior of trophoblast into the endometrium , and in particular , ( 2 ) MMP 2 may be the key regulator of trophoblast invasion in early human pregnancy . . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Although genes for TIMP 1 , 2 and 3 tended to be upregulated in AAA , relative expression levels of MMP 1 to TIMP 1 , MMP 1 to TIMP 2 , MMP 1 to TIMP 3 , and MMP 3 to TIMP 2 were still higher in AAA than in non dilated regions ( 1 . 12 + / 0 . 63 versus 0 . 10 + / 0 . 03 , 4 . 13 + / 1 . 12 versus 0 . 43 + / 0 . 11 , 1 . 61 + / 0 . 59 versus 0 . 14 + / 0 . 03 , and 7 . 81 + / 1 . 60 versus 2 . 56 + / 0 . 76 , respectively , P < 0 . 05 ) . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
MMP 2 activation by Actinobacillus actinomycetemcomitans supernatant in human PDL cells was corresponded with reduction of TIMP 2 . ^^^ Gelatin zymography , RT PCR and Western blot analysis were used to detect the activation of MMP 2 , expression of MT 1 MMP and TIMP 2 mRNA and the proteins , respectively . ^^^ CONCLUSION : The supernatant from A . actinomycetemcomitans and P . gingivalis could induce the activation of MMP 2 possibly through the imbalance of MT 1 MMP and TIMP 2 in HPDL cells but by different mechanisms . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
The transcripts levels of MMP 1 , MMP 2 , MMP 9 , TIMP 1 , and TIMP 2 relative to that of glyceraldehyde phosphate dehydrogenase were determined . ^^^ RESULTS : The transcripts levels of MMP 1 , MMP 2 , MMP 9 , TIMP 1 , and TIMP 2 were not correlated with age in the control group . ^^^ There were differences in the transcripts expression of MMP 2 ( P < 0 . 001 ) , MMP 9 ( P = 0 . 017 ) , TIMP 1 ( P < 0 . 001 ) , and TIMP 2 ( P = 0 . 001 ) among the control , primary open angle glaucoma , and primary angle closure glaucoma groups ( Kruscal Wallis H test ) . ^^^ Comparing with the controls , open angle glaucoma had decreased transcripts levels of MMP 2 ( P = 0 . 001 ) and TIMP 1 ( P = 0 . 006 ) while angle closure glaucoma had decreased transcripts levels of MMP 2 ( P < 0 . 001 ) , MMP 9 ( P = 0 . 004 ) , TIMP 1 ( P < 0 . 001 ) , and TIMP 2 ( P < 0 . 001 ) ( Mann Whitney U tests ) . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
We showed that treatment of cells with ConA activated pro MMP 2 , and that this activation concurred with elevated levels of cellular MT 1 MMP and TIMP 2 . ^^^ Inhibition of furin in ConA treated cells attenuated pro MT 1 MMP processing and the cellular TIMP 2 level , plus it reduced cell released active MMP 2 in a time dependent manner . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
METHODS : Male Sprague Dawley rats were randomly divided into normal , 4 d , 2 wk , 4 wk , 9 wk and 11 wk groups , and the model rats were fed with a mixture of alcohol by gastric infusion at the designed time , respectively , then decollated and their livers were harvested for the examination of MMP 2 , MMP 3 , MMP 9 , MMP 13 , TIMP 1 and TIMP 2 by immunohistochemistry , zymograghy and Western blotting , respectively . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
METHODS : Cell surface or / and intracellular expression of MMP 2 , 7 , 9 and MT 1 MMP and TIMPs ( TIMP 2 and 4 ) was determined in gastric epithelium and infiltrative mucosal lymphocytes ( IML ) in single endoscopic biopsies from H . pylori infected ( n = 25 ) and uninfected ( n = 15 ) patients . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
METHODS : Immunohistochemical expression of MMP 1 , MMP 2 , MMP 3 , MMP 7 , MMP 9 , TIMP 1 , and TIMP 2 was investigated in 33 human RMSs , 12 alveolar , and 21 embryonal histologic subtypes ( 12 high risk and 9 low / standard risk ) . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
MMP 2 , MMP 9 , MT 1 MMP , and extracellular matrix metalloproteinase inducer ( EMMPRIN ) , TIMP 1 , TIMP 2 , TIMP 3 , and TIMP 4 mRNA were quantitated by real time RCR . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
In this study , we investigated the synthesis of enzymes of collagen metabolism like collagen prolyl 4 hydroxylase ( P4H ) , matrix metalloproteinases ( MMP 2 and MMP 9 ) and their regulatory factors MT 1 MMP , TIMP 1 and TIMP 2 in HT 1080 fibroblasts under the influence of hypoxia . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
OBJECTIVES : This study aimed to analyze the expression of matrix metalloproteinases 2 ( MMP 2 ) and 9 ( MMP 9 ) and their tissue inhibitors TIMP 1 and TIMP 2 in low malignant potential ( LMP ) ovarian tumors and to compare these values with those recorded for benign and malignant ovarian neoplasms . ^^^ METHODS : A total of 53 ovarian tumors ( 16 benign , 15 LMP and 22 malignant ) were evaluated by immunohistochemistry for the expression of MMP 2 , MMP 9 , TIMP 1 and TIMP 2 . ^^^ CONCLUSION : The data suggest that , in relation to the expression of MMP 2 , MMP 9 , TIMP 1 and TIMP 2 , LMP ovarian tumors are more similar to benign than to malignant ovarian tumors . . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Expression of MMP 1 , MMP 2 , and MMP 9 and tissue inhibitors of metalloproteinases ( TIMP 1 and TIMP 2 ) were assessed by aortic tissue microarray immunostaining . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
The ratio of MMP 2 to TIMP 2 in hilar cholangiocarcinoma : a semi quantitative study . ^^^ The aim of this study was to evaluate the roles of matrix metalloproteinases 2 ( MMP 2 ) and its tissue inhibitor of metalloproteinase 2 ( TIMP 2 ) in tumor invasion or as a prognostic factor in patients with human hilar cholangiocarcinoma . ^^^ METHODS : The expressions of MMP 2 and TIMP 2 were investigated in patients . ^^^ The expressions of MMP 2 and TIMP 2 were examined immunohistochemically . ^^^ Image analysis with image pro plus analysis software was used to semiquantitatively determine the ratio of MMP 2 to TIMP 2 . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
The results clearly showed increased cell surface expression of MMP 2 , 7 , and 9 , MT 1 MMP , and TIMP 2 and 4 in both tumor cells and TIL during the development of invasion and / or metastasis of gastric carcinoma . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
In a rat tail suspension experimental model , we show that after fourteen days of non weight bearing there is increased expression of MMP 2 in the atrophic soleus and gastrocnemius and decreased expression of TIMP 2 . ^^^ In conclusion , an MMP 2 / TIMP 2 imbalance could have a role in the mechanism underlying muscle disuse atrophy ; more studies are needed to expand our molecular knowledge on this issue and to explore the possibility of targeting the proteolytic imbalance with MMP inhibitors . . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Clinical relevance of MMP 9 , MMP 2 , TIMP 1 and TIMP 2 in colorectal cancer . ^^^ Ninety five CRCs and their paired normal tissues were investigated to detect total levels of MMP 9 , MMP 2 , and the tissue inhibitors TIMP 1 and TIMP 2 . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Western blotting and immunohistochemistry revealed that MMP 1 , 2 , and 9 levels were higher and TIMP 1 and TIMP 2 levels were lower in regenerating compared to nonregenerating ON and retinae . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
SCH 66336 also inhibited the levels of secreted pro MMP 2 and pro MMP 9 as well as the release of their inhibitors TIMP 1 and TIMP 2 . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
We found an up regulation of TIMP 1 , TIMP 2 and MMP 2 RNA in DMD muscle . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
There was a positive correlation between MMP 2 and TIMP 2 ( r=0 . 399 , p < 0 . 001 ) , MMP 9 and TIMP 1 ( r=0 . 45 , p < 0 . 001 ) and between TIMP 1 and TIMP 2 ( r=0 . 275 , p=0 . 008 ) . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Plasma MMP 2 and MMP 9 activity , but not TIMP 2 , were significantly higher in patients than in controls ( median MMP 2 , 270 vs . 214 ng / mL , P=0 . 006 ; MMP 9 16 . 3 vs . 8 . 7 ng / mL , P < 0 . 0001 ) . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Coil occlusion , with or without recanalization , was followed by decreased expression of vWf , VE Cad , eNOS , VCAM 1 , MMP 2 , TIMP 1 , and TIMP 2 ; stable expression of PECAM 1 , SMA+ , and TIMP 3 ; and overexpression of Ang 1 and Ang 2 , MMP 9 , MMP 14 , and TIMP 4 . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
EXPERIMENTAL DESIGN : In this study all of the major members of the matrix metalloproteinase ( MMP 1 , MMP 2 , MMP 3 , MMP 7 , MMP 9 , MMP 13 , MT 1 MMP and MT 2 MMP ) / tissue inhibitor of matrix metalloproteinase ( TIMP 1 , TIMP 2 , and TIMP 3 ) system have been investigated by immunohistochemistry in a series ( n = 90 ) of stage 3 ( Dukes ' C ) colorectal cancers . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
CPL also down regulated the expression of urokinase type plasminogen activator ( uPA ) , one of key proteins associated with invasion and metastasis of tumor cells in a concentration dependent fashion , while CPL did n ' t significantly affect the expression of matrix metalloproteinase 2 ( MMP 2 ) and tissue inhibitor of metalloproteinase 2 ( TIMP 2 ) by reverse transcriptase polymerase chain reaction ( RT PCR ) . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Inflammatory markers ( tumour necrosis factor alpha , interleukin 8 , polymorphonuclear elastase ) , fibrinolytic system variables ( tissue plasminogen activator ( PA ) , urokinase PA ( u PA ) , plasminogen activation inhibitor ( PAI ) 1 , PAI 2 ) , and several MMPs ( MMP 1 , MMP 2 , MMP 8 , MMP 9 ) and TIMPs ( TIMP 1 , TIMP 2 ) were determined by ELISA in plasma and pleural fluid . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Genes such as gelatinase A ( MMP 2 ) , cathepsin L , tissue inhibitor metalloproteinases 2 ( TIMP 2 ) and 3 ( TIMP 3 ) , plasminogen activator inhibitor 1 ( PAI 1 ) , tissue type plasminogen activator ( tPA ) , urokinase plasminogen activator ( tPA ) , endothelin 1 , calponin , carboxypeptidase D and calponin acidic were down regulated . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
MMP 2 and TIMP 2 concentrations were measured by ELISA assays . ^^^ CONCLUSION : TIMP 2 is elevated in glaucomatous process over MMP 2 , which support and further extend the conjuncture that the ECM accumulation rather than degradation predominates in the pathogenesis of POAG and EXG . . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Aortic levels of MMP 2 , MMP 9 , TIMP 1 , and TIMP 2 were measured using ELISA . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
After 24 h , culture supernatants were obtained and assayed for MMP 2 , MMP 9 , tissue inhibitor of metalloproteinase ( TIMP ) 1 and TIMP 2 levels by ELISA . ^^^ RESULTS : FEX at more than 350 ng / mL inhibited the production of MMP 2 and MMP 9 from both PF and MF in response to TNF alpha stimulation , whereas TIMP 1 and TIMP 2 production was scarcely affected by FEX . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Although TIMP 2 secretion was enhanced in the Cel B group , MMP 2 / TIMP 2 ratios were unaffected . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Gene expression levels for furin , urokinase plasminogen activator , MMP 2 , MMP 9 , MT 1 MMP , and TIMP 2 were determined by real time PCR . ^^^ RESULTS : cPAF triggered significantly increased MT 1 MMP , MMP 2 , MMP 9 , and TIMP 2 mRNA expression , followed by increased active MT 1 MMP protein expression at 12 hours , whereas TIMP 2 protein increased at 24 hours . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
We demonstrated that normal thyrocytes constitutively express MMP 1 , MMP 2 , MMP 10 , MMP 14 , and TIMP 1 , TIMP 2 , TIMP 3 , and TIMP 4 , and this pattern of expression is profoundly modified in all thyroid tumor derived cell lines . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
RT PCR confirmed an increased levels of MMP 2 , MMP 9 , MMP 7 , MMP 14 , MMP 16 , and TIMP 1 , TIMP 2 in the spleen of HTS group compared to sham groups , whereas in lungs transcriptional levels of only TIMP 3 and TIMP 1 were significantly changed . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
METHOD : The expression of matrix metalloproteinase 2 , 9 ( MMP 2 , MMP 9 ) , tissue inhibitor 1 , 2 of matrix metalloproteinase ( TIMP 1 , TIMP 2 ) were determined in 60 patients with benign and malignant parotid gland tumors by ABC immunohistochemical methods , their correlation of clinical data was analysed . ^^^ RESULT : ( 1 ) Down regulation of TIMP 2 was accompanied by lymph node metastasis and distant metastasis in malignant parotid tumor ( P < 0 . 01 ) . ( 2 ) The expression of MMP 2 was higher in patients more than 60 years old in benign parotid tumor ( P < 0 . 01 ) . ( 3 ) There was a close positive relationship between the expression of MMP 2 and MMP 9 , MMP 9 and TIMP 1 , MMP 2 and TIMP 2 ( P < 0 . 05 ) . ^^^ CONCLUSION : The imbalance of MMP 2 and TIMP 2 may be more important in malignant parotid gland tumor , down expression of TIMP 2 may be an early biomarker of metastasis and invasion of tumor . . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Levels of MMP 2 , MMP 9 , MT 1 MMP , TIMP 1 and TIMP 2 in the kidneys were decreased in ICGN mice in comparison with normal ICR mice . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
The induction was time dependent , with effects primarily observed after 36 h ; under these conditions , MMP 2 , MMP 14 , and tissue inhibitor of MMP 1 ( TIMP 1 ) , TIMP 2 , and TIMP 3 were not induced . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Among the MMPs , the 72 kDa type 4 collagenase MMP 2 ( gelatinase A ) is activated in a membrane associated manner by an activation complex composed of membrane type 1 matrix metalloproteinase ( MT 1 MMP ) , tissue inhibitor of matrixmetalloproteinase 2 ( TIMP 2 ) , and pro MMP 2 in the presence of alphavbeta 3 integrin receptor . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Here we measured the expression and activity of MMPs and TIMPs , and tested the hypothesis that increased MMP activity plays a proapoptotic role in beta adrenergic receptor ( beta AR ) stimulated apoptosis of adult rat ventricular myocytes ( ARVMs ) . beta AR stimulation ( isoproterenol , 24 h ) increased mRNA levels of MMP 2 and TIMP 1 while it decreased TIMP 2 mRNA levels as analyzed by real time PCR . ^^^ Inhibition of MMPs using GM 6001 ( a broad spectrum inhibitor of MMPs ) , SB3CT ( inhibitor of MMP 2 ) , and purified TIMP 2 inhibited beta AR stimulated apoptosis as determined by TdT mediated dUTP nick end labeling staining . ^^^ These data suggest the following : 1 ) beta AR stimulation increases MMP 2 expression and activity and inhibits TIMP 2 expression ; 2 ) inhibition of MMPs , most likely MMP 2 , inhibits beta AR stimulated apoptosis ; and 3 ) the apoptotic effects of MMP 2 may be mediated , at least in part , via its interaction with beta 1 integrins and poly ADP ribose polymerase cleavage . . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
MEK inhibitors ( PD 98059 and U 0126 ) inhibited the production of MMP 2 , MMP 9 and high MW uPA , and upregulated TIMPs ( TIMP 1 , TIMP 2 and TIMP 3 ) . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
In this study , mRNA expression levels of MMP 1 , MMP 9 , TIMP 1 , and TIMP 2 were evaluated in patients with resected NSCLC , and their associations with disease progression and prognosis were determined . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Upregulation of endogenous tissue inhibitors of MMP ( TIMPs ) also provided ambiguous results , since TIMPs possess biologic functions in addition to MMP inhibition , for example , TIMP 2 is a main player in the MMP 2 activation cascade . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
We examined the effects of a high concentration of glucose and then focused on following four critical points : 1 ) the production of HGF from HPMCs exposed to a high concentration of glucose , 2 ) the expression of c Met on HPMCs , 3 ) the viability of those cells , and 4 ) matrix metalloproteinase 2 ( MMP 2 ) and tissue inhibitors of metalloproteinase 2 ( TIMP 2 ) . ^^^ The following significant changes are described herein : high glucose solution and TGF beta 1 1 ) decreased HGF production from HPMCs and 2 ) up regulated expression of c Met on HPMCs , and addition of HGF 3 ) restored viability of HPMCs damaged by glucose , 4 ) suppressed TGF beta 1 production by HGF , and 5 ) induced up regulation of MMP 2 and decreased TIMP 2 production by HPMCs . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
OBJECTIVE : To investigate the role of matrix metalloproteinase 2 , 9 ( MMP 2 , MMP 9 ) and tissue inhibitor of matrix metalloproteinases 2 , 1 ( TIMP 2 , TIMP 1 ) in human amniochorionic membrane . ^^^ Messenger ribonucleic acid expression for MMP 2 , MMP 9 , and their specific inhibitors TIMP 2 and TIMP 1 were studied with reverse transcriptase polymerase chain reaction . ^^^ CONCLUSIONS : In PROM group , the expression of MMP 2 , MMP 9 and their inhibitors TIMP 2 , TIMP 1 is imbalanced , leading to increased extracellular matrix degradation , and weakening of the fetal membranes , and eventually premature rupture of the membranes . . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
In the 1 yr high concentration group , gene expression of matrix metalloproteinase 2 ( MMP 2 ) and type 1 collagen mRNA in the rat lungs increased , while tissue inhibitors of metalloproteinase 2 ( TIMP 2 ) decreased . ^^^ The 2 yr high concentration group increased in message level of type 1 collagen and TIMP 2 but not that of MMP 2 . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
MMP 2 and TIMP 2 were determined to be the ambient protease and corresponding antiprotease of the microsomes . ^^^ However , H2O2 ( 1 mM ) caused changes in MMP 2 activity and Na+ dependent Ca2+ uptake were not reversed upon pretreatment of the microsomes with a low concentration of 5 microg / ml of TIMP 2 which otherwise reversed MMP 2 ( 1 microg / ml ) mediated increase in 14C gelatin degradation and inhibition of Na+ dependent Ca2+ uptake . ^^^ Direct treatment of TIMP 2 ( 5 microg / ml ) with H2O2 ( 1 mM ) abolished the inhibitory effect of the inhibitor on 14C gelatinolytic activity elicited by 1 microg / ml of MMP 2 . ^^^ Thus , one of the mechanisms by which H2O2 activates MMP 2 could be due to inactivation of TIMP 2 by the oxidant . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Both MMP 2 and MMP 9 were diminished , but no change was detected in TIMP 1 or TIMP 2 . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
The aim of this study was to investigate , by immunohistochemistry , the expression of the endothelial marker vWF , of angiogenic bFGF , VEGF and IL 8 , of collagenases MMP 2 and MMP 9 and their inhibitors TIMP 1 and TIMP 2 , in tissue samples from radiation induced chronic dermal wounds and healthy control skin . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
The expression of IL 1beta , IL 6 , TGF beta isoforms , ANF , and components of the extracellular matrix ( pro collagen 1 and 3 , colligin , MMP 2 and TIMP 2 ) was measured by ribonuclease protection assay . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
TIMP 1 , TIMP 2 , and MMP 2 were synthesized by the valvular interstitial cells in primary culture . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Active MMP 2 and TIMP 2 ( but not pro MMP 2 or pro or active MMP 9 ) levels are higher in the aorta than in the IMA ( P < . 001 ; P < . 05 ) . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
On the other hand , we conducted investigations on metalloproteinases ( MMP 2 , MMP 9 , MMP 7 ) and their specific tissue inhibitors TIMP 1 and TIMP 2 to verify their possible involvement in the etiopathogeny of SVAS and WBS . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Expression of PCNA , Ki 67 , MMP 2 and TIMP 2 proteins of GBC SD cells was determined by streptavidin biotin complex method . ^^^ With the increase in TIMP 2 expression , the MMP 2 to TIMP 2 ratio was decreased significantly ( P < 0 . 05 ) . ^^^ Its anti invasive activity may be the result of decrease in MMP 2 to TIMP 2 ratio and reduced cell motility . . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
In this study , we determined the levels of MMP 2 and MMP 9 and their inhibitors TIMP 1 and TIMP 2 in 19 patients who had malignant PE that was confirmed by histology or cytology and 30 patients who had nonmalignant , autoreactive PE compared with pericardial fluid of 19 patients who had preserved left ventricular function and who underwent aortocoronary bypass surgery for control . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
MMP 2 and TIMP 2 showed no significant differences at 5 weeks of age . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
During reperfusion , there was a gradual reversal of the MMP 2 and MMP 9 levels and a strong inhibition of TIMP 1 and TIMP 2 at the protein and mRNA levels . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Expression of MMPs ( MMP 2 , MMP 7 , MMP 9 , MT 1 MMP , MT 2 MMP , MT 3 MMP ) and tissue inhibitors of metalloproteinase ( TIMP : TIMP 1 , TIMP 2 ) was investigated on cultured hepatocellular carcinoma ( HCC ) cells and surgically resected HCC tissues . ^^^ The cultured cells and tissues expressed MMPs and TIMPs at various degrees , and high expression was observed for MMP 2 , MMP 9 , MT 1 MMP and TIMP 2 . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
TIMP 2 is also involved in the activation of MMP 2 on the cell surface . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
The levels of VEGF , bFGF , MMP 2 , MMP 9 , TIMP 1 and TIMP 2 were measured using a sandwich enzyme immunoassay kit . ^^^ The levels of VEGF , bFGF , MMP 2 , MMP 9 , TIMP 1 and TIMP 2 did not change significantly after gefitinib treatment , even in responders . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
The serum MMP 2 , MMP 8 , MMP 9 and TIMP 2 levels were not different in all groups . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Human cardiac myocytes and human cardiac fibroblasts also express MMP 1 , 2 , 3 and 9 , and TIMP 2 constitutively . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Associated changes in the transcription of MMP 2 , TIMP 2 , and CTGF were assessed by semiquantitative PCR . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
RESULTS : All patients who died from their disease process had significantly greater MMP 2 expression in their lymph nodes relative to TIMP 2 expression . ^^^ In contrast , patients with prolonged disease free survival had decreased nodal MMP 2 / TIMP 2 expression ( P = 0 . 001 ) . ^^^ Patients with relatively higher MMP 2 / TIMP 2 expression in their tumors also had a worse prognosis ( P = 0 . 06 ) . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
MMP 9 , MMP 2 , tissue inhibitor of MMP ( TIMP ) 1 , TIMP 2 , Bax , and BCl 2 proteins in retinal extracts were determined by Western blot analysis . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
We tested the effect of depsipeptide ( DP ) on UM cell migration and the level and activity of MMP 2 , MMP 9 , MT 1 / MMP and tissue inhibitors of matrix metalloproteinases 1 and 2 ( TIMP 1 and TIMP 2 ) . ^^^ Enzyme linked immunosorbent assays ( ELISAs ) were used to quantify the level of MMP 2 , MMP 9 , MT 1 / MMP , TIMP 1 and TIMP 2 after the cells had been exposed to DP ( 0 , 1 , 5 and 10 nmol / l ) for 24 h . ^^^ We detected a dose dependent : ( 1 ) decrease in the protein level of MMP 2 , MMP 9 and MT 1 / MMP ; ( 2 ) decrease in the activity of MMP 2 , MMP 9 and MT 1 / MMP ; and ( 3 ) increase in the protein level of TIMP 1 and TIMP 2 . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
AIM : To explore the relationship between matrix metalloproteinase 2 ( MMP 2 ) and tissue inhibitor of metalloproteinase 2 ( TIMP 2 ) in the development of colorectal carcinoma and to provide a valuable marker for clinical diagnosis . ^^^ Western blot and ABC immunohistochemical staining were used to detect the expression levels of MMP 2 and TIMP 2 in normal and colorectal carcinoma tissues . ^^^ Western blot and ABC immunohistochemical staining demonstrated that the expression level of MMP 2 in colorectal carcinoma tissues was much higher than that in normal tissues ( P < 0 . 05 , t = 9 . 429 ) , but the expression level of TIMP 2 in colorectal carcinoma tissues was much lower than that in normal tissues ( P < 0 . 05 , t = 7 . 329 ) . ^^^ The MMP 2 / TIMP 2 ratio of colorectal carcinoma was much higher than that of normal tissues . ^^^ With the progression of invasion depth , lymph node metastasis and tumor Duke ' s stage , the activity and expression level of MMP 2 and TIMP 2 gradually increased , but the MMP 2 / TIMP 2 ratio gradually decreased . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
RPM treatment was associated with increased activation of pro MMP 9 , a significant decrease in MMP 2 tissue levels , and corresponding increases in TIMP 2 and TIMP 3 expression . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
The apparent lack of MMP 2 autoactivation with or without calcium , likely resulted from the coexistence of its bound endogenous inhibitor , TIMP 2 . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
CONCLUSION : TIMP 2 gene transfer locally in atherosclerotic plaque could inhibit the activities of MMP 2 and MMP 9 in the lesion , increase the thickness of fibrous cap and the amount of collagen of the lesion , but may have no effect on the degree of the stenosis . . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
The mRNA content of collagen type 1 , collagen type 3 , MMP 2 , TIMP 1 , TIMP 2 , TIMP 3 and TIMP 4 was measured by semi quantitative reverse transcription polymerase chain reaction ( RT PCR ) and normalized to beta actin or GAPDH . ^^^ The mRNA of MMP 2 was significantly correlated with the mRNA of TIMP 2 ( r = 0 . 326 , P = 0 . 006 ) , the mRNA of collagen type 1 ( r = 0 . 322 , P = 0 . 006 ) , left atrial dimension ( r = 0 . 300 , P = 0 . 011 ) and AF duration ( r = 0 . 300 , P = 0 . 010 ) . ^^^ CONCLUSION : The increased level of collagen type 1 associated with selective downregulation of TIMP 2 and upregulation of MMP 2 gene expression in atrium could be one of the molecular mechanisms of atrial fibrosis during atrial fibrillation , which correlates with the initiation and maintenance of AF . . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Based on previous MMP cloning studies from our laboratory , the current study has focused on the expression of membrane type 1 metalloproteinase ( MT 1 MMP ) , gelatinase A ( MMP 2 ) and endogenous tissue inhibitor 2 of metalloproteinases ( TIMP 2 ) during fin regeneration in adult zebrafish . ^^^ Matrix metalloproteinase expression and function during fin regeneration in zebrafish : analysis of MT 1 MMP , MMP 2 and TIMP 2 . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
However , CFs secreted comparatively lower levels of MMP 1 , MMP 2 and TIMP 1 , and nearly equal levels of TIMP 2 . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
METHODS : DNA samples from 812 unrelated white subject ( AAA , n = 387 ; controls , n = 425 ) were genotyped for 14 polymorphisms in 13 different candidate genes : MMP 1 ( nt 1607 ) , MMP 2 ( nt 955 ) , MMP 3 ( nt 1612 ) , MMP 9 ( nt 1562 ) , MMP 10 ( nt+180 ) , MMP 12 ( nt 82 ) , MMP 13 ( nt 77 ) , TIMP 1 ( nt+434 ) , TIMP 1 ( rs 2070584 ) , TIMP 2 ( rs 2009196 ) , TIMP 3 ( nt 1296 ) , TGFB 1 ( nt 509 ) , ELN ( nt+422 ) , and COL3A1 ( nt+581 ) . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Correlation between expression of MMP 2 , MMP 9 , TIMP 2 , TIMP 1 and metastasis of neuroblastoma ] . ^^^ OBJECTIVE : To study MMP 2 , MMP 9 , TIMP 2 and TIMP 1 expression , and their association to invasion and metastasis of neuroblastoma ( NB ) . ^^^ METHODS : The staining status was compared of MMP 2 , MMP 9 , TIMP 2 and TIMP 1 in cryostat sections of tumor tissue in 35 NB patients by immunohistochemistry . ^^^ CONCLUSION : MMP 2 , MMP 9 expression may be related to metastasis and progression of neuroblastoma , while TIMP 2 may have an inhibitory effect . . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Blood samples were drawn before tissue plasminogen activator treatment and an MMP array ( multiplex enzyme linked immunosorbent assay [ ELISA ] ) was performed including gelatinases ( MMP 2 and MMP 9 ) , collagenases ( MMP 1 , MMP 8 , and MMP 13 ) , stromelysines ( MMP 3 and MMP 10 ) , and MMP endogen inhibitors ( TIMP 1 and TIMP 2 ) . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Cultured fibroblasts from the dermis of normal subjects and of Pseudoxanthoma elasticum ( PXE ) patients were analysed for enzyme activity , protein and mRNA expression of metalloproteases ( MMP 2 , MMP 3 , MMP 9 , MT 1 MMP ) and of their specific inhibitors ( TIMP 1 , TIMP 2 and TIMP 3 ) . ^^^ MMP 2 mRNA was significantly more expressed in PXE than in control cell lines , whereas MT 1 MMP , TIMP 1 and TIMP 2 mRNAs appeared unchanged . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Transient exposure to low shear stress upregulated TGF beta 1 , MMP 2 , 14 , and TIMP 2 gene expression while MMP 9 expression was downregulated . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Protein expressions of interstitial collagenase ( MMP 1 ) , gelatinase ( MMP 2 ) and tissue inhibitors of matrix metalloproteinases 2 ( TIMP 2 ) were assessed by Western blot . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
CAPE subcutaneous administration ( 15 micromol / kg / day ) improved cardiac cytoarchitecture , decreased the levels and the expression of MMP 2 , and increased those of TIMP 2 proteins . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Cell surface tTG activity was reduced in TSP 2 null cells ( 0 . 50 + / 0 . 05 arbitrary units versus 0 . 84 + / 0 . 07 for wild type ; P < or = 0 . 05 ) , and addition of MMP 2 to the culture medium of wild type cells caused a 35 % reduction in cell surface tTG activity . tTG was susceptible to proteolysis by MMP 2 in vitro , and addition of the MMP inhibitor TIMP 2 to TSP 2 null cells restored tTG activity ( 0 . 3 + / 0 . 08 for untreated cells ; 0 . 71 + / 0 . 09 with TIMP 2 ) . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Immunohistochemical staining of MMP 2 , MMP 9 , TIMP 1 , TIMP 2 , and urokinase type plasminogen activator were performed . ^^^ RESULTS : The depth of submucosal invasion was not significantly correlated with the expression of MMP 2 , MMP 9 , TIMP 1 , TIMP 2 , or urokinase type plasminogen activator , but the microvessel count was significantly correlated with the absolute depth of invasion ( r=0 . 312 , p < 0 . 05 ) . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
The results showed that cyanidin 3 glucoside and cyanidin 3 rutinoside treatments could decrease the expressions of matrix matalloprotinase 2 ( MMP 2 ) and urokinase plasminogen activator ( u PA ) in a dose dependent manner and enhance the expression of tissue inhibitor of matrix matalloprotinase 2 ( TIMP 2 ) and plasminogen activator inhibitor ( PAI ) . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
We detected the mRNA expressive levels of MMP 2 , MMP 9 and their natural inhibitors TIMP 1 , TIMP 2 in Caco 2 cells by reverse transcription polymerase chain reaction ( PCR ) technique . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Prognostic value of stromal immunolabelling by MMP 2 , MT 1 MMP and TIMP 2 in clinically localized prostate cancer ] . ^^^ The objective of this study was to determine the prognostic value of stromal immunolabelling with MMP 2 , MT 1 MMP and TIMP 2 in clinically localized prostate cancer . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Regulation of TIMP 2 , MT 1 MMP , and MMP 2 expression during C2C12 differentiation . ^^^ To investigate the role of MMP mediated proteolysis during myogenesis , the regulation of TIMP 2 , MT 1 MMP , and MMP 2 expression was investigated during differentiation of the mouse myoblastic C2C12 cell line . ^^^ The more diffuse spatial distribution of TIMP 2 relative to MT 1 MMP and MMP 2 suggests that TIMP 2 may exert MMP independent functions during myogenesis . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Membrane type 1 matrix metalloproteinase ( MT 1 MMP ) and the diffusible matrix metalloproteinase MMP 2 , in the presence of the tissue inhibitor of metalloproteinases TIMP 2 , constitute a system of proteins that play an important role during the proteolysis of collagen type 1 matrices . ^^^ The cell expresses MMP 2 in its proenzyme form , pro MMP 2 , as well as MT 1 MMP and TIMP 2 . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
The concentrations of MMP 1 , 2 , 3 , 8 , 9 , 13 , TIMP 1 and TIMP 2 were quantified using ELISA . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Decreased TIMP 2 scores in patients with a direct inguinal hernia , compared with both the indirect inguinal hernia group and the control group , explain the reason for the increase in matrix metalloproteinase 2 ( MMP 2 ) that has been proposed in some studies . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
OBJECTIVE AND DESIGN : To clarify the role of ovarian steroids in the differential regulation of MMP 1 , MMP 3 , MMP 7 , MMP 8 , MMP 10 , TIMP 1 , TIMP 2 , and TIMP 3 mRNAs , we compared their variations in the cycling endometrium in vivo with their response to hormone addition or withdrawal in corresponding explants . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
EGCG upregulated the expression of tissue inhibitor of MMP 2 ( TIMP 2 ) protein . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Regression analysis showed a very high R 2 between MMP 2 and TIMP 2 . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Matrix metalloproteinases and their tissue inhibitors in basal cell and metatypical cancer of the skin ] . 10 cases of ulcerative nodular basal cell carcinoma and 10 cases of metatypical carcinoma of the skin were studied immunohistochemically for immunoexpression of matrix metalloproteinases ( MMP 1 , MMP 9 ) and their endogenic tissue inhibitors ( TIMP 1 , TIMP 2 ) in combination with PCNA , p 53 tumor complexes . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Moreover , latanoprost led to a delayed up regulation of MMP 1 mRNA , whereas the expression of MMP 2 , MMP 9 , TIMP 1 , and TIMP 2 remained unchanged . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
In order to establish whether the higher metastatic potential of ARMS cells may depend on differential expression of specific matrix metalloproteinases ( MMPs ) and angiogenesis related factors , we studied the expression of MMP 2 , MT 1 MMP , TIMP 2 , VEGF and VEGF receptors in four ARMS ( RH 30 , RH 4 , RH 18 , RH 28 ) , three ERMS ( RD , RH 36 , SMS CTR ) and one undifferentiated sarcoma cell line ( A 204 ) . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Expression and activity of MMP 1 , MMP 2 , MMP 3 , MMP 9 , TIMP 1 , TIMP 2 and MT 1 MMP were analysed in those cells cultured in contact with one of the relevant protein component of the dermal matrix , collagen type 1 as well as in monolayer . ^^^ Expression of MMP 1 , TIMP 1 , TIMP 2 and MT 1 MMP was similar in all cells , in both culture conditions , whereas expression of MMP 3 was absent in dermal papilla cells . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Given the role of MT 1 MMP and TIMP 2 in MMP 2 activation , and the upregulation of MMP 9 , these data suggest an important role for matrix remodeling , particularly basement membrane , in this progression . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Levels of MMP 2 ( p < 0 . 0005 ) and TIMP 2 ( p < 0 . 01 ) , were elevated in ischemic samples . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Quantitative estimation of matrix metalloproteinases 2 and 7 ( MMP 2 , MMP 7 ) and tissue inhibitors of matrix metalloproteinases 1 and 2 ( TIMP 1 , TIMP 2 ) in colorectal carcinoma tissue samples . ^^^ Matrix metalloproteinases ( MMPs ) and tissue inhibitors of matrix metalloproteinases ( TIMPs ) , in particular MMP 2 , MMP 7 , TIMP 1 and TIMP 2 , play an important role in the process of ECM and BM degradation in connection with tumor invasion . ^^^ The aim of our study was to assess the levels of MMP 2 , MMP 7 , TIMP 1 and TIMP 2 mRNA expression in colorectal carcinoma tissue samples and to correlate them with the stage of the disease . ^^^ The expression levels of mRNA MMP 2 , MMP 7 , TIMP 1 , TIMP 2 and glyceraldehyde 3 phosphate dehydrogenase ( GAPDH ) , as housekeeping gene , were quantified in tissue samples using the method of reverse transcription real time PCR . ^^^ In addition the presence of mRNA MMP 2 , MMP 7 , TIMP 1 and TIMP 2 in tumor tissue samples in these parameters was significantly higher than in the control tissue ( p < 0 . 003 , p < 0 . 0001 , p < 0 . 0001 and p < 0 . 05 ) . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
The organ culture media were analyzed using Western blottings ( MMP 1 , MMP 2 , MMP 3 , MMP 7 , MMP 9 , MMP 13 , TIMP 1 , and TIMP 2 ) and gelatine zymography . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Cardiac mRNA expression of TIMP 1 and TIMP 2 was significantly increased in AS compared with control patients ( mRNA transcript levels normalized to GAPDH : TIMP 1 , 0 . 67+ / 0 . 1 in AS versus 0 . 37+ / 0 . 08 in control patients ; TIMP 2 , 9 . 5+ / 2 . 6 in AS versus 1 . 6+ / 0 . 4 in control patients ; P < 0 . 05 for both ) but did not differ significantly for MMP 1 , 2 , or 9 . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
After 24 hours , the culture supernatants were obtained and assayed for MMP 2 , MMP 9 , TIMP 1 , and TIMP 2 levels by ELISA . ^^^ TR at more than 5 10 10 ( 5 ) M inhibited the production of MMP 2 and MMP 9 from NF in response to TNF alpha stimulation , whereas TIMP 1 and TIMP 2 production was scarcely affected . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
RESULTS : MMP 2 , TIMP 1 , and TIMP 2 ELISA concentrations were increased in CA pleural fluid vs . ^^^ CONCLUSIONS : MMP 2 , TIMP 1 , and TIMP 2 accumulate in the pleural compartment in CA and CHF , probably reflecting an unspecific pleural reaction . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
OBJECTIVE : In the present study , the significance of circulating matrix metalloproteinases 2 and 9 ( MMP 2 , MMP 9 ) , as well as their tissue inhibitors 1 and 2 ( TIMP 1 , TIMP 2 ) in ovarian cancer were studied to assess the possibility of using them in clinical decision making . ^^^ No statistically significant correlation was found between the circulating gelatinases ( MMP 2 , MMP 9 ) or TIMP 2 and the clinicopathological factors . ^^^ However , a tendency for better survival with high serum concentration of TIMP 2 or MMP 2 was observed . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Exposure of neonatal mice to chronic hypoxia ( 10 % O 2 ) , a stimulus that leads to an arrest of lung development , resulted in upregulation of MMP 2 with a concomitant downregulation of TIMP 2 . ^^^ MMP 2 , TIMP 2 , and TIMP 3 may be key regulatory enzymes during lung development , possibly through their complex action on ECM components , membrane receptor ectodomain shedding , and growth factor bioactivity . . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Helicobacter pylori infection , the invasion and metastasis of the gastric carcinoma , and the expression of MMP 2 and TIMP 2 ] . ^^^ OBJECTIVE : To explore the relationship among Helicobacter pylori ( H . pylori ) infection , the invasion and metastasis of the gastric carcinoma , and the MMP 2 and TIMP 2 expression . ^^^ The expression of MMP 2 and TIMP 2 in 22 patients with gastric carcinoma infected by H . pylori and 22 patients with gastric carcinoma but not infected were detected by immunohistochemistry method . ^^^ The positive rates of MMP 2 expression in the invasive group ( 96 . 6 % ) and in the metastasis group ( 100 . 0 % ) were significantly higher than that in the non invasive group ( 40 . 0 % ) and the non metastasis group ( 50 . 0 % ) , whereas the positive rates of TIMP 2 expression in the invasive group ( 48 . 3 % ) and in the metastasis group ( 45 . 8 % ) were significantly lower than that in the non invasive group ( 86 . 7 % ) and the non metastasis group ( 80 . 0 % ) . ^^^ The ratios of MMP 2 / TIMP 2 in the invasive group ( 6 . 86 ) and in the metastasis group ( 7 . 94 ) were significantly higher than that in the non invasive group ( 0 . 87 ) and the non metastasis group ( 1 . 07 ) . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
OBJECTIVE : To detect the expression of MMP 2 , MMP 9 and TIMP 2 in pituitary tumors and to explore the relationship among the expressions of MMP 2 , MMP 9 , TIMP 2 and invasiveness of pituitary adenomas . ^^^ METHODS : The expressions of MMP 2 , MMP 9 and TIMP 2 were determined in invasive pituitary adenomas ( n=20 ) and non invasive pituitary adenomas ( n=10 ) by using immunohistochemical method and comparative reverse transcription polymerase chain reaction ( RT PCR ) . ^^^ The correlation between expressions of MMP 2 , MMP 9 , TIMP 2 and tumor invasiveness were studied . ^^^ There was a reverse correlation between MMP 2 and TIMP 2 at mRNA level . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
The aim of the present study was to evaluate the effect of latanoprost monotherapy on the aqueous humour concentrations of TGF beta 1 , MMP 2 , TIMP 2 , MMP 9 and gelatinolytic activity in patients treated for exfoliative glaucoma ( XFG ) . ^^^ The concentrations of TGF beta 1 , MMP 2 , TIMP 2 , MMP 9 and gelatinase activity were determined by commercial immunoassays . ^^^ Latanoprost monotherapy has a marked effect on the aqueous concentration of TGF beta 1 , MMP 2 and TIMP 2 in XFG patients . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
The aim of this study is to investigate the expression of matrix metalloproteinase 2 ( MMP 2 ) and the tissue inhibitor of metalloproteinase 2 ( TIMP 2 ) in oral squamous cell carcinoma ( OSCC ) and adjacent normal tissues , and explore the role of MMP 2 and TIMP 2 in carcinoma metastasis and invasion . ^^^ Expression of MMP 2 and TIMP 2 was evaluated in 40 cases with semi quantitative reverse transcriptase polymerase chain reaction ( RT PCR ) and immunohistochemical techniques . ^^^ The values of MMP 2 / beta actin and TIMP 2 / beta actin in OSCC were significantly higher than those in adjacent normal tissues 2 cm and > or = 5 cm from carcinoma tissues ( p < 0 . 05 ) . ^^^ The results of immunohistochemical analysis show that the positive expression of MMP 2 and TIMP 2 protein in tumor tissues ( 60 . 0 and 52 . 5 % ) was higher than that in adjacent normal tissues 2 cm ( 30 . 0 and 22 . 5 % ) and 5 cm ( 22 . 5 and 25 . 0 % ) from cancer tissues , and the difference was significant p < 0 . 05 ) . ^^^ The expression of MMP 2 and TIMP 2 was related to the differentiation degree of tumor cells , metastatic lymph node status and stage of carcinoma , but not related to the age and gender of patients , or location of the carcinoma . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
In this study , we investigated : ( 1 ) whether the level of matrix degradation correlated with the duration of the symptomatic disease , ( 2 ) roles of matrix metalloproteinase 2 ( MMP 2 ) and tissue inhibitor of matrix metalloproteinases 2 ( TIMP 2 ) in intervertebral disc degeneration . ^^^ Alterations in biochemical components of extracellular matrix in intervertebral disc herniation : role of MMP 2 and TIMP 2 in type 2 collagen loss . ^^^ Nucleus pulposus of intervertebral discs were obtained from 22 patients and analysed for collagen and proteoglycan contents , and pro MMP 2 , TIMP 2 levels . ^^^ Pro MMP 2 and TIMP 2 levels negatively correlated in herniated discs samples ( r = 0 . 855 , P < 0 . 01 ) . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
The expression of MMP 2 , MMP 3 , MMP 9 , TIMP 1 , and TIMP 2 was examined at the messenger RNA and protein levels . ^^^ RESULTS : In SS patients the MMP 3 / TIMP 1 ratio was higher than 1 and the MMP 9 / TIMP 1 ratio was much higher than 1 whereas the MMP 2 / TIMP 2 ratio nearly equaled 1 , suggesting elevated proteolytic activity due mainly to MMP 9 . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
TIMP 2 , tissue inhibitor of MMP 2 , produced a similar increase in FAK phosphorylation , whereas treatment of ARVMs with purified active MMP 2 significantly inhibited FAK phosphorylation . ^^^ Furthermore , inhibition of MMP 2 using SB3CT and TIMP 2 attenuated beta AR stimulated decreases in mitochondrial membrane potential . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
In addition to the effect on MMP secretion / activation , VEGF stimulated secretion of TIMP 2 ; and blocking TIMP 2 activity by an anti TIMP 2 MAb significantly increased VEGF ( 80 ng / ml ) induced DOV 13 invasion ( p < 0 . 05 ) , suggesting that VEGF may regulate MMP 2 activity in DOV 13 conditioned medium through TIMP 2 . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
The activities of MMP 2 and MMP 9 and the abundance of TIMP 1 and TIMP 2 in LV tissue were increased at 18 weeks of age , and pravastatin also prevented these changes . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
We therefore investigated the effects of Ang 2 dependent hypertension on MMP 2 , MMP 9 , TIMP 1 , and TIMP 2 in isolated glomeruli of 8 week old homozygous male rats overexpressing the mouse Ren 2 gene [ TGR ( mRen 2 ) 27 ] . ^^^ Quantification of mRNA levels in isolated glomeruli by real time polymerase chain reaction showed a significant increase of TGF beta 1 , a 2 . 3 and a 2 . 6 fold increase of MMP 2 and TIMP 1 in Ren 2 compared with SD ( p < 0 . 01 , respectively ) and no strain differences for TIMP 2 . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
RESULTS : Enzymography and immunoblotting revealed increased MMP 2 as well as increased levels of TIMP 1 and TIMP 2 . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
INTRODUCTION : Alteration in the expression of extracellular matrix metalloproteinase inducer ( EMMPRIN ) , matrix metalloproteinase 2 ( MMP 2 ) , tissue inhibitors of matrix metalloproteinases ( TIMP 2 ) and platelet derived growth factor ( PDGF AA ) may contribute to poor healing in venous leg ulcers . ^^^ AIM : The aim of this study is to determine the expression of EMMPRIN , MMP 2 , TIMP 2 and PDGF AA in the ulcer exudates and perivascular tissue of healing and non healing chronic venous ulcers . ^^^ Immuno histochemistry using monoclonal antibodies to PDGF AA , MMP 2 , TIMP 2 and EMMPRIN was carried out on paraffin embedded punch biopsy skin specimens from the ulcer edge . ^^^ Enzyme linked immunosorbent assay for PDGF , MMP 2 and TIMP 2 were carried out on wound fluids collected from patients . ^^^ RESULTS : No significant difference was seen in the expression of TIMP 2 , MMP 2 and EMMPRIN between the two groups . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
The endothelium of all vessels and the arterial smooth muscle cells expressed MMP 1 , 2 , 9 , TIMP 2 and 3 , but not TIMP 1 , at all developmental stages . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Purpose of this study is to investigate the spatial localization or regulation of matrix metalloproteinases ( MMP 2 , 7 9 , MT 1 MMP ) and their inhibitors ( TIMP 2 and 4 ) on myofibroblasts from malignant ascites in 20 patients with gastric carcinoma . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Sebum also contains MMP 1 , MMP 13 , TIMP 1 , and TIMP 2 , as assessed by ELISA and western blot , but only MMP 13 was decreased following treatment with isotretinoin . ^^^ The origin of MMP and TIMP in sebum is attributed to keratinocytes and sebocytes , since we found that HaCaT keratinocytes in culture secrete proMMP 2 , proMMP 9 , MMP 1 , MMP 13 , TIMP 1 , and TIMP 2 . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
METHODS : Competitive RT PCR was used to measure the levels of mRNA for MT 1 MMP , MMP 2 , MMP 3 , TIMP 1 , TIMP 2 , and TIMP 3 in the scleras of tree shrews that had received either 1 , 2 , 4 , or 11 days of monocular 5 D lens treatment , or 11 days of 5 D lens treatment followed by 2 or 4 days of recovery . ^^^ CONCLUSIONS : The observed changes in MT 1 MMP , MMP 2 , TIMP 2 , and TIMP 3 mRNA are consistent with visually modulated MT 1 MMP activation of MMP 2 and with MT 1 MMP degradation of scleral extracellular matrix components . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
One set of genes consistently upregulated in anterior segment tissues from different patients with PEX comprised latent transforming growth factor binding proteins ( LTBP 1 and 2 ) , which are structural components of elastic microfibrils , the cross linking enzyme transglutaminase 2 ( TGase 2 ) , tissue inhibitor of matrix metalloproteinase 2 ( TIMP 2 ) , A kinase anchor protein 2 ( AKAP 2 ) , apolipoprotein D , and the adenosine receptor A 3 ( AdoR A 3 ) . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
We have tested the hypothesis that progressive cell clustering controls the proteolytic cascade for activation of gelatinase A / matrix metalloproteinase 2 ( MMP 2 ) , which involves formation of an intermediate ternary complex of membrane type 1 MMP ( MT 1 MMP / MMP 14 ) , tissue inhibitor of metalloproteinase 2 ( TIMP 2 ) , and pro MMP 2 at the cell surface . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
On this line , we examined the influence of CAPE on the gene expression of MMPs ( MMP 2 , MMP 9 , MT 1 MMP ) , tissue inhibitor of metalloproteinase 2 ( TIMP 2 ) and in vitro invasiveness of human fibrosarcoma cells . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
As expected , pressure overload hypertrophy was associated with interstitial fibrosis ( collagen : 4 . 37+ / 1 . 23 % BVh versus 1 . 23+ / 0 . 44 % SVh , P < 0 . 05 ) , which was abolished by sympathectomy ( 2 . 55+ / 1 . 31 % , P=not significant versus SSx ) but left unchanged by beta blockade ( 4 . 11+ / 1 . 23 % , P < 0 . 05 versus both SBb and BSx ) . beta blockade , but not sympathectomy , was also associated with an increased TIMP 2 / MMP 2 ratio ( P < 0 . 05 ) , indicating reduced interstitial collagenolytic activity . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Gelatin zymography and Western blot analyses showed that latent and activated MMP 2 and MMP 9 collagenases , and tissue inhibitor of metalloproteinase 2 ( TIMP 2 ) were present in all malignant fluids . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
We aimed to evaluate whether in cultured human vascular smooth muscle cells ( hVSMC ) CRP modulates the synthesis and release of metalloproteinase 2 ( MMP 2 ) , which is deeply involved in plaque instabilization and vascular remodeling , and of the tissue inhibitor of metalloproteinase 2 ( TIMP 2 ) . ^^^ Both in supernatants and in cell lysates of cultured hVSMC exposed to CRP ( 0 10 mg / L ) , we evaluated MMP 2 activity ( gelatin zymography ) , MMP 2 and TIMP 2 protein synthesis ( immunoblotting ) , MMP 2 and TIMP 2 mRNA expression ( reverse transcription polymerase chain reaction ) . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Molecular analysis using reverse transcription polymerase chain reaction , Western immunoblotting , gelatin zymography , and reverse zymography were performed to study the expression and activation of MMP 2 and MMP 9 , and tissue inhibitors of MMP ( TIMP ) 1 and TIMP 2 . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
After ganglion neuron axotomy , both MMP 2 activity and protein level increased , whereas the level of its mRNA was unchanged , suggesting prominent MMP 2 posttranslational regulation . mRNA and protein levels of the enzymes involved in the MMP 2 activation pathway , the membrane type 1 MMP ( MT 1 MMP ) , and the tissue inhibitor of metalloproteinase 2 ( TIMP 2 ) also increased after injury with a time course that correlated with that of MMP 2 activation . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
TIMP 2 and TIMP 3 expression were enhanced in CTX ( TIMP 2 , 195 % + / 42 % of CON , P = . 02 ; TIMP 3 , 118 % + / 3 % of CON , P = . 002 ) , and correspondingly , gelatinase MMP 2 activity was reduced ( P < . 05 ) . ^^^ The TIMP : MMP ratio was increased in CTX hearts ( TIMP 2 to MMP 2 , 410 % + / 134 % of CON , P = . 04 , and TIMP 3 to MMP 9 , 205 % + / 47 % of CON , P = . 03 ) , reflecting a reduced capacity for matrix degradation . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
METHODS : Of 48 laryngeal squamous cell carcinoma ( LSCC ) biopsies and 10 HNSCC cell lines , mRNA was isolated , reversely transcribed , and subjected to polymerase chain reaction ( PCR ) amplifying MMP 1 , MMP 2 , MMP 9 , MMP 10 , TIMP 1 , and TIMP 2 . ^^^ RESULTS : Densitometric analysis revealed MMP 2 expression and lymph node metastases to be positively and TIMP 1 and TIMP 2 to be negatively correlated with lymph node metastases . ^^^ CONCLUSIONS : Our results suggest that MMP 2 expression enhances , whereas TIMP 1 and TIMP 2 both suppress , cancer spread in LSCC . . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
In this study , we examined the influence of DA 125 , a new anthracyclin analog , on the gene expression of MMPs ( MMP 2 , MMP 9 and MT 1 MMP ) , tissue inhibitor of metalloproteinases ( TIMP 1 and TIMP 2 ) and in vitro invasiveness of human fibrosarcoma cells . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Single nucleotide polymorphisms identified in the promoters of MMP 2 ( 1306C > T ) and TIMP 2 ( 418G > C ) abolish the Sp 1 binding site and thus may down regulate expression of the genes . ^^^ MMP 2 genotypes were determined by PCR based allele specific refractory mutation analysis and TIMP 2 genotypes identified by PCR RFLP in a panel of HNSCC cell lines and in 239 head and neck cancer patients and 250 frequency matched controls in an ethnic Thai population . ^^^ Furthermore , the polymorphisms in both genes showed some additive effect and the highest risk for head and neck cancer was observed in those with MMP 2 CC genotype and TIMP 2 variant GC or CC genotype ( OR , 2 . 34 ; 95 % CI , 1 . 31 4 . 18 ) . ^^^ These findings suggest that the genetic polymorphisms in the promoters of MMP 2 and TIMP 2 may be associated with the development and aggressiveness of HNSCC . . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Significantly higher expression levels of MMP 2 , 11 , 13 , 14 , 15 , and 24 , and TIMP 1 and 3 were found in the brain and spinal cord 1 week after birth compared to later time points , while MMP 9 and TIMP 2 upregulation was restricted to the brain . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
We characterized the role of inflammatory mediators and extracellular signal regulated kinases ( ERKs ) in the control of arterialized vein graft expression of MMP 9 , MMP 2 , and membrane type 1 MMP ( MT 1 MMP ) and of the tissue inhibitor of metalloproteinases 2 ( TIMP 2 ) . ^^^ Vein graft arterialization resulted in rapid and sustained ( 8 h to 28 days ) upregulation of vein graft associated MMP 9 , MMP 2 , MT 1 MMP , thrombin activity , and TNF alpha levels with concomitant TIMP 2 downregulation . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Induction of myopia leads to a decreased glycosaminoglycan synthesis and smaller collagen fibrillar diameters , increased levels of gelatinase A ( MMP 2 ) and decreased amounts of tissue inhibitor of matrix metalloproteinase 2 ( TIMP 2 ) in the fibrous sclera of both chicks and tree shrews . ^^^ Changes in scleral MMP 2 , TIMP 2 and TGFbeta 2 mRNA expression after imposed myopic and hyperopic defocus in chickens . ^^^ The aim of the current study was to measure MMP 2 , TIMP 2 and TGFbeta 2 mRNA expression changes separately in the two scleral layers of chicks , following myopic and hyperopic defocus . ^^^ Real time PCR was used to measure MMP 2 , TIMP 2 and TGFbeta 2 mRNA levels . ^^^ Few changes in MMP 2 and TIMP 2 mRNA levels were measured following treatment with plus and minus lenses for up to 3 days . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Notably , none of the cultures altered the secretion of TIMP 2 , or MMP 1 and 2 , in response to MMC . hmECs cultured within collagen gels elongated significantly after exposure to MMC , a response the authors concluded was mediated by TIMP 1 , because elongation could be inhibited completely with a function blocking antibody to TIMP 1 . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Pretreatment of the smooth muscle with SOD ( the O 2 * scavenger ) and TIMP 2 ( MMP 2 inhibitor ) prevented the increase in MMP 2 activity and PKC activity , and reversed the inhibition of Na+ dependent Ca2+ uptake in the microsomes . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
The mRNA levels of preproET 1 , endothelin converting enzyme ( ECE 1 ) , ETa receptor and ETb receptor , matrix metalloproteinase ( MMP 2 ) , tissue inhibitor of MMP ( TIMP 2 ) , and heat shock protein ( HSP 70 ) were determined by quantitative real time RT PCR . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Expression levels of MMP 2 , 9 , MT 1 MMP and TIMP 2 were examined immunohistochemically in samples from 47 patients with osteosarcoma . ^^^ Co localization of MMP 2 , MT 1 MMP and TIMP 2 was determined by double staining with fluorescence conjugated antibodies . ^^^ MMP 2 was expressed strongly in 60 % of cases ( 28 / 47 ) , and MMP 9 , MT 1 MMP and TIMP 2 was strongly positive in 61 % ( 29 / 47 ) , 45 % ( 21 / 47 ) , and 91 % ( 43 / 47 ) , respectively . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Expression of flk , flt 1 , the matrix metalloproteinase mt 1 mmp , and the tissue inhibitor of metalloproteinase timp 2 , but not of mmp 2 , mmp 9 , timp 1 , or timp 3 , correlates with inner retinal vascularization . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Moreover , R ( + ) MA increased the mRNA and protein expression of MMP 1 , MMP 3 , MMP 9 , and TIMP 1 but not that of MMP 2 and TIMP 2 . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
In addition , we clearly detected the messenger ribonucleic acids ( mRNA ) of four matrix metalloproteinases ( MMP 2 , MMP 3 , MMP 10 , and MMP 13 ) and of two tissue inhibitors of matrix metalloproteinases ( TIMP 1 and TIMP 2 ) in hepatocytes from both normal and cirrhotic rats by RT PCR and by in situ hybridization . ^^^ Finally , we demonstrated MMP 2 , MMP 3 , and MMP 13 and TIMP 1 and TIMP 2 proteins in the same hepatocyte preparations by immunostaining . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Plasma levels of VEGF , MMP 2 , TIMP 1 , TIMP 2 and C reactive protein ( CRP ) were measured in these 12 patients 12 months after discharge during a silent clinical phase , in 12 age and sex matched patients with stable angina ( SA ) and coronary artery disease , and in 12 age and sex matched patients with normal coronary arteries ( NCA ) . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
MATERIALS AND METHODS : Serum levels of MMP 2 , MMP 9 , TIMP 1 , and TIMP 2 were measured in 23 women with PCOS [ age ( mean + / sd ) , 30 . 5 + / 6 . 7 yr ; body mass index , 35 . 8 + / 7 . 5 kg / m2 ] and 22 healthy , regularly menstruating women ( age , 29 . 4 + / 5 . 6 ; body mass index , 31 . 7 + / 9 . 2 kg / m2 ) . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Aortic smooth muscle cell release of matrix metalloproteinase 2 ( MMP 2 ) and tissue inhibitor of metalloproteinase 2 ( TIMP 2 ) has been implicated in aortic aneurysm pathogenesis , but proximal modulation of release is poorly understood . ^^^ We hypothesized that nucleotides modulate MMP 2 and TIMP 2 release from human aortic smooth muscle cells ( HASMCs ) via distinct purinergic receptors and signaling pathways . ^^^ MMP 2 and TIMP 2 released into supernatant were assessed using ELISA and Western blotting . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
METHODS : The expression of MMP 2 , MMP 9 , MMP 13 , tissue inhibitors of metalloproteinase ( TIMP ) 1 and TIMP 2 was analyzed by immunohistochemistry in the lesional skin of BP patients in comparison with that in normal human skin . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
In the present study , we have demonstrated that CCL 23 enhances the expression of MMP 2 mRNA and protein levels in endothelial cells in a dose dependent manner , but has no effect on the expression levels of MMP 9 , TIMP 1 , TIMP 2 , and MT 1 MMP . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
A significant increase in pro MMP 2 expression was seen in vaginal tissue from women with prolapse ( P < 0 . 05 ) but not activated MMP 2 , MMP 9 and TIMP 2 . ^^^ Significant correlations exist in expression of pro MMP 2 , activated MMP 2 , MMP 9 and TIMP 2 in vaginal tissue with that in uterosacral ligaments . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
RESULTS : In normal endometrium , epithelial expression of MMP 2 ( P = 0 . 0007 ) , MMP 7 ( P = 0 . 0002 ) and TIMP 2 ( P = 0 . 0004 ) was increased during the proliferative phase of the menstrual cycle . ^^^ MMP 2 expression correlated negatively with TIMP 2 expression ( P = 0 . 001 , rho = 0 . 702 ) . ^^^ In hyperplastic endometrium , the presence of atypia was associated with lower TIMP 2 expression ( P = 0 . 005 ) and was also associated with a trend towards higher MMP 2 expression . ^^^ In endometrial carcinomas , MMP 2 expression increased ( P = 0 . 0004 ) and TIMP 2 expression decreased ( P = 0 . 0005 ) with the histological grade . ^^^ Strong MMP 2 and weak TIMP 2 expression were the most potent markers of endometrial malignancies with a high risk of local and distant spread . . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Staining for MMP 2 and TIMP 2 did not change significantly . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
RT PCR was performed to determine the mRNA levels of matrix metalloproteinase 2 ( MMP 2 ) and tissue inhibitor metalloproteinase 2 ( TIMP 2 ) . ^^^ In addition , after treatment with L NAME , expression of MMP 2 mRNA was significantly decreased ( t = 71 . 238 , P < 0 . 01 ) and that of TIMP 2 mRNA was markedly increased ( t = 13 . 020 , P < 0 . 01 ) . ^^^ CONCLUSION : L NAME exerts anti invasive and anti metastatic effects on SL 174T cell line via downregulating MMP 2 mRNA expression and upregulating TIMP 2 mRNA expression . . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Tissue and circulating immunoreactive protein for MMP 2 and TIMP 2 in head and neck squamous cell carcinoma tissue immunoreactivity predicts aggressive clinical course . ^^^ In this study , the clinical significance of tumor tissue and circulating immunoreactive proteins for MMP 2 and TIMP 2 was assessed in HNSCC . ^^^ Expression of MMP 2 and TIMP 2 was examined in paraffin embedded tumor sections by immunohistochemical methods using specific antibodies . ^^^ The pretreatment serum levels of MMP 2 , TIMP 2 and MMP 2 : TIMP 2 complex were quantitatively measured by ELISA assay . ^^^ Serum levels of MMP 2 , TIMP 2 or MMP 2 : TIMP 2 complex failed to associate with the clinical behavior of HNSCC in this material . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
We evaluated the location , origin and activity of MMP 2 in cervical squamous cell carcinomas in comparison with MT 1 MMP ( MMP 14 ) , TIMP 2 and extracellular matrix metalloproteinase inducer ( EMMPRIN ) . ^^^ The MMP 2 staining of TBS tumors correlated significantly with the presence of TIMP 2 and MT 1 MMP , proteins involved in docking MMP 2 to the cell surface and essential for MMP 2 activation . ^^^ This pro MMP 2 could subsequently be activated on tumor cells via the presence of MT 1 MMP and TIMP 2 . ^^^ In conclusion , the colocalization of EMMPRIN , MT 1 MMP and TIMP 2 in human cervical carcinomas seems to be involved in a specific distribution pattern of tumor cell bound MMP 2 , which is related with local proteolytic activity and therefore might be associated with worse prognosis of the patients . . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
METHODS AND RESULTS : Six widely invasive follicular carcinomas ( WIFCs ) , 15 minimally invasive follicular carcinomas ( MIFCs ) , 19 follicular adenomas ( FAs ) and 10 adenomatous goitres ( AGs ) were analysed immunohistochemically for MMP 1 , MMP 2 , MMP 7 , MMP 9 , membrane type 1 MMP ( MT 1 MMP ) and tissue inhibitor of matrix metalloproteinase 2 ( TIMP 2 ) . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Serum MMP 9 / TIMP 1 and MMP 2 / TIMP 2 ratios in multiple sclerosis : relationships with different magnetic resonance imaging measures of disease activity during IFN beta 1a treatment . ^^^ We studied the relationships between serum MMP 9 , MMP 2 , TIMP 1 and TIMP 2 and different magnetic resonance imaging ( MRI ) measures of disease activity in MS patients during treatment with rIFNbeta 1a . ^^^ Serum MMP 9 , MMP 2 , TIMP 1 and TIMP 2 were measured , MMP 9 to TIMP 1 and MMP 2 to TIMP 2 ratios were calculated and the numbers of Gd SD , Gd TD , new Gd SD , new Gd TD and new T 2 lesions counted . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Twenty four hours after labeling , MMP 1 , 2 , and 3 and TIMP 2 secretion increased ( P < 0 . 001 ) with RT PCR confirmation at 12 hours , whereas TIMP 1 did not . ^^^ CONCLUSIONS : After labeling with these maghemite nanoparticles , HGF increased secretion of IL 1beta at D 1 , probably inducing the increase of MMP 1 , 2 , and 3 and TIMP 2 . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
The cell surface and / or intracellular localization of MMP 2 , 7 , and 9 , membrane type 1 ( MT 1 ) MMP and TIMP 2 and 4 was quantitatively determined by flow cytometry in living ( non permeabilized ) and permeabilized tumor cell lines . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
The importance of MT 1 MMP , in particular , is highlighted by its ability to activate pro MMP 2 at the cell surface through the formation of a trimolecular complex comprised of MT 1 MMP / tissue inhibitor of metalloproteinase 2 ( TIMP 2 ) / pro MMP 2 . ^^^ Exogenous TIMP 2 and N TIMP 3 inhibited this activation , whereas TIMP 3 containing matrix from wild type MEFs did not rescue the enhanced MMP 2 activation in Timp 3 ( / ) cells . ^^^ Increased processing of MMP 2 did not arise from increased expression of MT 1 MMP , MT 2 MMP , or MT 3 MMP or altered expression of TIMP 2 and MMP 2 . ^^^ To test whether increased MMP 2 processing in Timp 3 ( / ) MEFs is dependent on TIMP 2 , double deficient Timp 2 ( / ) / 3 ( / ) MEFs were used . ^^^ In these double deficient cells , the cleavage of pro MMP 2 to its intermediate form was substantially increased , but the subsequent cleavage of intermediate MMP 2 to fully active form , although absent in Timp 2 ( / ) MEFs , was detectable with combined Timp 2 ( / ) / 3 ( / ) deficiency . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
BMP 2 also reduced the secretion of MMP 9 into the culture media , but did not affect the secretion of MMP 2 , Timp 1 , Timp 2 or Timp 3 . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
We investigated whether a mitogenic bovine whey extract enriched with growth factors modulated the expression and activity of MMP 2 and 9 , and the tissue inhibitor of MMP 2 ( TIMP 2 ) in chronic leg ulcers . ^^^ Immunohistochemical analysis of ulcer biopsies for MMP 2 , 9 , and TIMP 2 expression showed a reduction in the number of MMP 2 positive dermal fibroblasts in the mitogenic bovine whey extract treated ulcers compared with pretreatment biopsies ( p < 0 . 05 ) that persisted over the course of the study . ^^^ These results show that topical application of mitogenic bovine whey extract was able to modulate the expression of MMP 2 , 9 , and TIMP 2 in chronic leg ulcers and that its constituent growth factors may have the potential to redress the proteolytic imbalance observed in nonhealing chronic ulcers . . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
This effect was associated with a reduced expression of MMP 2 and u PA , together with an enhanced expression of TIMP 2 and PAI 1 . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
The expression levels of MMP 1 , MMP 2 , MMP 14 , and TIMP 1 markedly exceeded the control levels at 1 . 0g / cm ( 2 ) of compressive force , whereas the expression levels of MMP 3 , MMP 13 , TIMP 2 , TIMP 3 , TIMP 4 , tPA , uPA , and PAI 1 markedly exceeded the control levels at 3 . 0g / cm ( 2 ) . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
After 24 h , the culture supernatants were obtained and assayed for MMP 1 , MMP 2 , MMP 3 , MMP 13 , TIMP 1 and TIMP 2 by ELISA . mRNA expression for MMPs and TIMPs in 4 h cultured cells were examined by real time polymerase chain reaction . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Gelatin zymography , Western blot , and real time PCR analysis showed that TGF beta 1 enhanced the expression and secretion of MMP 2 , TIMP 1 , and , to a lesser degree , MMP 9 but not membrane type 1 MMP and TIMP 2 . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
A much lower level of mRNA expression of TIMP 1 and TIMP 2 was detected in the atherosclerotic plaque lesions of MMP 2 ( / ) : apoE ( / ) mice than in those of MMP 2 ( + / + ) : apoE ( / ) mice . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Retinal pigment epithelium protection from oxidant mediated loss of MMP 2 activation requires both MMP 14 and TIMP 2 . ^^^ Supernatants and cell lysates were collected for analysis of MMP 2 , MMP 14 , and TIMP 2 activity , mRNA and protein expression . ^^^ In contrast , overexpression of MMP 14 together with the addition of exogenous TIMP 2 prevented the reduction of MMP 2 activation . ^^^ CONCLUSIONS : Loss of MMP 2 activity after oxidant injury is caused by the downregulation of MMP 14 and TIMP 2 . ^^^ Overexpression of either MMP 14 or TIMP 2 alone before oxidant injury is not enough to prevent loss of MMP 2 activity . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Both MMP 2 and MMP 9 and TIMP 1 and TIMP 2 grade decreased with time ( P < . 01 ) in all groups , with no differences between groups . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Analysis of gene expression by microarray and Northern blot analysis revealed the MEK / ERK target genes clusterin , matrix metalloproteinase 2 ( Mmp 2 ) , peptidylpropyl isomerase C associated protein , syndecan 4 , Timp 2 and Thbs 1 to be repressed in the HRAS transformed FE 8 cells in a MEK / ERK and methylation dependent manner . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
IFN beta suppressed the TNF alpha induced levels of secreted MMP 9 and MMP 2 , while enhancing the expression of TIMP 1 and TIMP 2 mRNA . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
To better understand the mechanisms responsible for the pathological processes of osteoarthritis ( OA ) and to potentially identify a profile of changes that could be predictive of early OA , matrix metalloproteinase 1 ( MMP 1 ) and tissue inhibitor of matrix metalloproteinase 2 ( TIMP 2 ) in the synovial fluid and serum of normal and osteoarthritic dogs were examined . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
In situ gene expression and localization of metalloproteinases MMP 1 , MMP 2 , MMP 3 , MMP 9 , and their inhibitors TIMP 1 and TIMP 2 in human renal cell carcinoma . ^^^ In this study the expression pattern of MMP 1 , MMP 2 , MMP 3 , MMP 9 , and their inhibitors TIMP 1 , and TIMP 2 were investigated at mRNA and protein levels in human renal cell carcinoma ( RCC ) . ^^^ MMP 3 mRNA and antigen were expressed moderately to strong in tumour epithelium and focally in stroma cells . mRNA or TIMP 1 and TIMP 2 mRNA and antigen were also predominantly expressed in tumour epithelium ; only few samples showed positive expression in stroma cells . mRNA expression could be generally correlated to the protein expression in our study group , except for MMP 1 ( mRNA expression was only expressed in two cases ) . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
The stroke group had lower MMP 2 concentrations and MMP 2 / TIMP 2 ratios ( p < 0 . 001 ) but higher TIMP 2 ( p < 0 . 001 ) than controls . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
The mRNA expression of MMP 2 , MT 1 MMP and TIMP 2 were analyzed by real time reverse transcription polymerase chain reaction . ^^^ Correlation of mRNA expression of MMP 2 , MT 1 MMP and TIMP 2 was investigated in tumors with different histological subtypes and clinical stages . ^^^ RESULTS : There were no significant differences in the expressions of MMP 2 , MT 1 MMP and TIMP 2 mRNA between 1 and 2 stage or 3 and 4 stage thymomas ( P > 0 . 05 ) . ^^^ Expression levels of MT 1 MMP and TIMP 2 mRNA were correlated with pro MMP 2 activation ratio ( Spearman rank correlation : r = 0 . 7235 , r = 0 . 7647 , P < 0 . 005 ) . ^^^ CONCLUSIONS : MMP 2 , MT 1 MMP and TIMP 2 mRNA expression levels are correlated with the histologic subtypes and clinical stages of thymoma . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Matrix metalloproteinase 2 ( MMP 2 ) and its tissue inhibitor ( TIMP 2 ) are prognostic factors in cervical cancer , related to invasive disease but not to high risk human papillomavirus ( HPV ) or virus persistence after treatment of CIN . ^^^ OBJECTIVE : Matrix metalloproteinase 2 ( MMP 2 ) and its tissue inhibitor ( TIMP 2 ) are important regulators of cancer invasion and metastasis . ^^^ Matrix metalloproteinase 2 ( MMP 2 ) and its tissue inhibitor ( TIMP 2 ) are prognostic factors in cervical cancer , related to invasive disease but not to high risk human papillomavirus ( HPV ) or virus persistence after treatment of CIN . ^^^ MATERIALS AND METHODS : As part of our HPV PathogenISS study , a series of 150 CCs and 152 CIN lesions were examined using immunohistochemical ( IHC ) staining for MMP 2 and TIMP 2 and tested for HPV using PCR with 3 primer sets ( MY09 / 11 , GP5+ / GP6+ , SPF ) . ^^^ Thus , the MMP 2 : TIMP 2 ratio increased with progressive CIN , exceeding the value 1 . 0 only in invasive disease . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
The HUVECs were treated with various concentrations of BDNF ( 25 400 ng / ml ) for different ( 6 48 hours ) , reverse transcriptase polymerase chain reaction ( RT PCR ) was used to assay MMP 2 , MMP 9 , TIMP 1 , and TIMP 2 mRNA in HUVECs , and the conditioned medium was analyzed for MMP and uPA activity by gelatin zymography and fibrin zymography , respectively . uPA , plasminogen activator inhibitor ( PAI ) 1 , tissue inhibitors of metalloproteinase ( TIMP ) 1 , and TIMP 2 were quantified by western blotting analysis . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
In fact , gene array analyses demonstrated that the proapoptotic cytokine tumour necrosis factor related apoptosis inducing ligand ( TRAIL ) inhibited mRNA expression of VEGF , along with those of matrix metalloproteinase 2 ( MMP 2 ) , its inhibitor tissue inhibitor of matrix metalloproteinases 2 ( TIMP 2 ) , as well as the tumour invasiveness related gene secreted protein acid rich in cysteine ( SPARC ) in different human glioblastoma cell lines . ^^^ Moreover , the expression of MMP 2 , its inhibitor TIMP 2 and the tumour invasiveness related protein SPARC were effectively inhibited by TRAIL in glioblastoma cell lines . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
The arterial endothelium and medial smooth muscle expressed MMP 2 , 9 and TIMP 2 in both CDH and control lungs . ^^^ In small arteries ( < 100 microm in diameter ) , the positive surface area of MMP 2 , 9 and TIMP 2 was significantly larger in CDH lungs than in controls . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
The effects of As ( 2 ) O ( 3 ) on MMP 2 , MMP 9 , TIMP 1 and TIMP 2 gene expressions and protein expressions of tumor cells were determined by RT PCR and ELISA , respectively . ^^^ As ( 2 ) O ( 3 ) inhibited mRNA and protein expressions of MMP 2 , MMP 9 and TIMP 2 of tumor cells . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
CK 5 correlated with tissue inhibitor of metalloproteinase ( TIMP ) 1 , but not matrix metalloproteinase ( MMP ) 1 , MMP 2 , extracellular matrix metalloproteinase inducer , TIMP 2 or plasminogen activator inhibitor , indicating that antiproteolytic stimuli might be preponderant in these neoplasms . . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Stomachs were assessed for macroscopic injury ( computed planimetry ) , and gastric mucosal MMP production was assessed by gelatin zymography , in situ zymography , and Western analysis for MMP 2 , MT 1 MMP , and TIMP 2 . ( n > or = 4 / group ; ANOVA ) . ^^^ RESULTS : Aminoguanidine treatment decreased LPS induced macroscopic gastric injury as well as MMP 2 and MT 1 MMP protein production while having no effect on TIMP 2 protein levels . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
OBJECTIVE : This study evaluated the secretions of zymogen and active forms of matrix metalloproteinase ( MMP ) 9 and MMP 2 and their specific inhibitors , TIMP 1 and TIMP 2 by fetal membranes stimulated with group B Streptoccocci ( GBS ) . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Serum alanine aminotransferase , histologic changes , gelatinolytic activity , MMP 2 and MMP 9 activities , tissue inhibitor of metalloproteinase 2 ( TIMP 2 ) messenger RNA ( mRNA ) levels , and mRNA and serum levels of tumor necrosis factor alpha ( TNFalpha ) and interleukin 1beta ( IL 1beta ) were measured in both groups . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Collectively , these results demonstrate that submicromolar concentrations of methylselenol increase both prometastasis MMP 2 and MMP 9 and antimetastasis TIMP 1 and TIMP 2 expression . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Plasma levels of MMP 2 and 9 and TIMP 1 and TIMP 2 were measured by ELISA methods . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
The present study used an in vitro flow perfused BBB model to analyze the individual contributions of flow , cytokine levels , and circulating blood leukocytes on the release / activity of MMP 9 , MMP 2 , and their endogenous inhibitors , the tissue inhibitors of MMPs ( TIMPs ) , TIMP 1 , and TIMP 2 . ^^^ The presence of circulating blood leukocytes under normoxic / normoglycemic flow cessation / reperfusion significantly increased the luminal levels of MMP 9 and activity of MMP 2 , accompanied by partial reduction of TIMP 1 , complete reduction of TIMP 2 and increased BBB permeability . ^^^ The addition of anti IL 6 or anti TNF alpha antibody in the lumen before reperfusion suppressed the levels of MMP 9 and activity of MMP 2 , had no effect on TIMP 1 , and completely restored TIMP 2 and BBB integrity . ^^^ Injection of TIMP 2 in the lumen before reperfusion prevented the activation of MMP 2 and BBB permeability . ^^^ These data indicate that blood leukocytes and loss of flow are major factors in the activation of MMP 2 , and that cytokine mediated differential regulation of TIMP 1 and TIMP 2 may contribute significantly to BBB failure . . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Furthermore , the expression of matrix metalloproteinase 2 ( MMP 2 ) and tissue inhibitor of metalloproteinase 2 ( TIMP 2 ) was detected by RT PCR in these cells . ^^^ Moreover , the expression of MMP 2 mRNA was significantly reduced in the SSTR 2 expressing cells and conversely the expression of TIMP 2 mRNA was significantly increased in the SSTR 2 expressing cells when compared with the Adv GFP control and mock control ( P < 0 . 01 ) . ^^^ The expression of reintroduced human SSTR 2 gene in BXPC 3 cells by Adv GFP SSTR 2 had the anti migratory and anti invasive effects , and the mechanisms involved in this effect may be due to the down regulated expression of MMP 2 and up regulated expression of TIMP 2 . . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
However , the effects of these mediators on matrix metalloproteinase 2 ( MMP 2 ) and on tissue inhibitor of metalloproteinase 2 ( TIMP 2 ) in proximal tubule cells have yet to be fully examined within the context of early stage diabetic nephropathy . ^^^ METHODS : In this study , we attempted to characterize changes in MMP 2 and TIMP 2 in streptozotocin induced diabetic rats . ^^^ To further examine the molecular mechanisms involved , we evaluated the effects of high glucose ( 30 mM ) or Ang 2 on MMP 2 , TIMP 2 and collagen synthesis in proximal tubule cells , and investigated whether MMP 2 and TIMP 2 are regulated via the TGF beta 1 pathway . ^^^ Urinary protein excretion also showed a significant positive correlation with glomerular and tubular TIMP 2 protein expressions , and a negative correlation with MMP 2 expression . ^^^ In cultured cells , both high glucose and Ang 2 induced significant increases in TGF beta 1 , TIMP 2 , and in collagen synthesis , and significant decreases in MMP 2 gene expression and activity , and thus disrupted the balance between MMP 2 and TIMP 2 . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
In human choriocarcinoma cells ( BeWo ) , resistin ( 10 100 ng / ml ) enhanced both MMP 2 protein and mRNA expression , significantly reduced TIMP 1 and TIMP 2 and increased trophoblast like cell invasiveness . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
The present study was undertaken to investigate the activity of matrix metalloproteinase 2 ( MMP 2 ) , the expression of MMP 2 and tissue inhibitor of metalloproteinase 2 ( TIMP 2 ) in kidney tissues of diabetic rats , and to explore the degradative pathway of type 4 collagen ( 4 C ) and the renal protective effects of ACE inhibition benazepril . ^^^ The levels of MMP 2 , TIMP 2 and collagen 4 ( 4 C ) protein in the kidney tissue were assessed by immunohistochemistry . ^^^ The gene expression of MMP 2 and TIMP 2 was measured by reverse transcription polymerase chain reaction ( RT PCR ) . ^^^ In the DM group , the mRNA , enzymatic activity and proteins of MMP 2 decreased , but the expressions of 4 C and TIMP 2 increased . ^^^ CONCLUSIONS : The changes of MMP 2 and TIMP 2 expressions in kidney tissues of diabetes rats may contribute to the occurrence and progression of diabetic nephropathy . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
METHODS : We performed gelatin zymography , northern blot analysis and immunohistochemistry to compare the expression levels of MT 1 MMP , MMP 2 , matrix metalloproteinase 9 ( MMP 9 ) and the tissue inhibitors of MMPs 1 and 2 ( TIMP 1 and TIMP 2 ) in the rat uterus 18 h , 36 h and 5 days after parturition with their expression levels during pregnancy ( day 20 ) . ^^^ CONCLUSION : These expression patterns indicate that MT 1 MMP , MMP 2 , MMP 9 , TIMP 1 and TIMP 2 may play key roles in uterine postpartum involution and subsequent functional regenerative processes . . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Finally , the role of MMP 2 and TIMP 2 in AoSMC migration was confirmed silencing MMP 2 and TIMP 2 in young and aged AoSMCs , respectively ; therefore , in this study we showed that these enzymes play a pivotal role in the regulation of the AoSMC migration during in vitro aging . . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Baseline MMP 2 and TIMP 2 serum levels were lower and MMP 9 and TIMP 1 serum levels higher in all patients compared to age matched controls . ^^^ Eight days after IVIG treatment , MMP 2 , TIMP 2 , and TIMP 1 serum levels increased , while MMP 9 serum levels decreased , indicating tissue repair . ^^^ After 60 days , MMP 9 levels increased , MMP 2 approached normal levels , while TIMP 1 and TIMP 2 serum levels were below day 8 levels , indicating relapsing tissue damage . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
The influence of MMP 14 , TIMP 2 and MMP 2 expression on breast cancer prognosis . ^^^ METHODS : MMP 2 , MMP 14 and TIMP 2 expression has been evaluated by 35S mRNA in situ hybridization on paraffin material of 539 breast cancers without distant metastasis at diagnosis and with a median follow up of 9 . 2 years . ^^^ RESULTS : MMP 2 and MMP 14 mRNA was detected primarily in reactive stromal cells whereas TIMP 2 mRNA was expressed by both stromal and cancer cells . ^^^ Furthermore , when all three components were analyzed together , the survival was worst for patients with high MMP 2 / high MMP 14 / low TIMP 2 ( 5 year survival = 60 % ) and best with low MMP 2 / low MMP 14 / high TIMP 2 ( 5 year survival = 74 % ) , but the difference did not reach statistical significance ( p = 0 . 3285 ) . ^^^ CONCLUSION : Of the MMP 14 / TIMP 2 / MMP 2 complex , MMP 14 was the factor most significantly associated with the outcome of breast cancer and was an independent factor of poor overall survival when adjusted for clinical prognostic factors , but not for certain ancillary markers . . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
MMP 2 and TIMP 2 levels were analyzed by enzyme linked immunosorbent assay . ^^^ RESULTS : Enzyme linked immunosorbent assay measurements of both MMP 2 and TIMP 2 were statistically significantly increased in the USG samples relative to POAG and cataracts ( P=0 . 002 ) . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
In addition , we transfected a TIMP 2 plasmid into the gynecologic cancer cell lines SKOV 3 , 2774 , and HeLa and then assayed cell growth , apoptosis , and MMP 2 activation . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Immunohistochemistry with paraffin blocks and real time RT PCR analysis for MMP 2 , MMP 9 , TIMP 1 , TIMP 2 and vascular endothelial growth factor ( VEGF ) were performed using stored frozen tissues . ^^^ MMP 2 , TIMP 1 , TIMP 2 and VEGF were weakly expressed or not expressed in both microcystic and non microcystic meningiomas . ^^^ The expression of MMP 2 ( 0 . 72 + / 1 . 20 vs . 2 . 54 + / 3 . 01 , p = 0 . 01 ) and TIMP 2 ( 1 . 22 + / 1 . 67 vs . 1 . 61 + / 1 . 82 , p = 0 . 02 ) was higher in the control group . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
MMP 9 and MMP 2 gelatinases and TIMP 1 and TIMP 2 inhibitors in breast cancer : correlations with prognostic factors . ^^^ We evaluated the activity and the expression levels of MMP 9 , MMP 2 , TIMP 1 and TIMP 2 in malignant versus benign fresh breast tumor extracts . ^^^ In addition , MMP 9 / TIMP 1 and MMP 2 / TIMP 2 ratio values obtained by us were significantly different in malignant tumors compared to benign tumors . ^^^ We suggest that the abnormal MMP 9 / TIMP 1 balance plays a role in the configuration of breast invasive carcinoma of no special type and also in tumor growth , while altered MMP 2 / TIMP 2 ratio value could be associated with lymph node invasion and used as a prognostic marker in correlation with Nottingham Prognostic Index . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Expression of MT 1 MMP , MMP 2 , MMP 9 and TIMP 2 mRNAs in ductal carcinoma in situ and invasive ductal carcinoma of the breast . ^^^ We investigated the expression of membrane type 1 ( MT 1 ) MMP , MMP 2 , MMP 9 and TIMP 2 mRNAs and their roles in ductal carcinoma in situ ( DCIS ) and T 1 and T 2 invasive ductal carcinoma of the breast . ^^^ MT 1 MMP , MMP 2 , MMP 9 and TIMP 2 mRNA were expressed in both DCIS and invasive ductal carcinomas . ^^^ Expression rates of MT 1 MMP , MMP 2 , MMP 9 and TIMP 2 mRNAs were not statistically different between DCIS and invasive ductal carcinomas , nor did they differ statistically when grouped by tumor size , histologic grade or nuclear grade of invasive ductal carcinoma . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
In ATA , MMP 2 , MMP 9 , TIMP 1 , TIMP 2 , TIMP 3 , and osteopontin expression in SMCs was higher than in inflammatory cells ( median score : 3 versus 0 , P < . 0001 ; 4 versus 1 , P < . 0005 ; 2 versus 0 , P < . 001 ; 5 versus 2 , P < . 0001 ; 2 versus 0 , P < . 005 ; and 5 versus 1 . 5 , P < . 0001 , respectively ) , when both inflammatory cells of the media and the adventitia were considered together . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
The specimens were analyzed by immuno histochemistry and ELISA for MMP 2 , MMP 9 , TIMP 1 and TIMP 2 . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
METHODS : In this study the concentrations of MMP 1 , MMP 2 , MMP 3 , MMP 9 , their inhibitors TIMP 1 , TIMP 2 , and the MMP1 / TIMP1 complex ( MTC 1 ) were quantified in blood plasma with the sandwich enzyme linked immunosorbent assay ( ELISA ) . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
MMP 2 and MMP 9 levels were examined using gelatin zymography and Western blotting ; their endogenous inhibitors , tissue inhibitor of metalloproteinase 2 ( TIMP 2 ) and tissue inhibitor of metalloproteinase 1 ( TIMP 1 ) , were assessed by Western blotting . uPA , uPAR and PAI 1 were examined using enzyme linked immunosorbent assay ( ELISA ) . ^^^ RESULTS : In both colon and rectal tumors , MMP 2 , MMP 9 and TIMP 1 protein levels were higher than in corresponding paired normal mucosa , while TIMP 2 level in tumors was significantly lower than in normal mucosa . ^^^ CONCLUSION : These findings suggest that uPA may be expressed differentially in colon and rectal cancers , however , the activities or protein levels of MMP 2 , MMP 9 , TIMP 1 , TIMP 2 , PAI 1 and uPAR are not affected by tumor location in the colon or the rectum . . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
In adenocarcinoma areas , positive activity was observed with both primary antibodies and probes for matrix metalloproteinase 2 ( MMP 2 ) in 11 / 50 patients ( 22 % ) , membrane type 1 matrix metalloproteinase ( MT 1 MMP ) in 39 / 50 patients ( 78 % ) and tissue inhibitor of metalloproteinase 2 ( TIMP 2 ) in 49 / 50 patients ( 98 % ) . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Moreover , antioxidants reversed the suppression of MMP 2 expression by upregulation of MT 1 MMP and downregulation of TIMP 2 . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Latanoprost increased MMP 1 ( in four of five cultures ) , MMP 3 ( in four of five cultures ) , MMP 17 ( in three of five cultures ) , MMP 24 ( in all five cultures ) , TIMP 2 , 3 , and 4 expression ( in three of five cultures ) ; MMP 11 and 15 were downregulated . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Supernatants and cell homogenates were collected to assess MMP 2 and TIMP 2 activity by zymography and reverse zymography , proteins by Western blot , and type 4 collagen accumulation by ELISA and immunostaining . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
A quantitative enzyme linked immunosorbent assay was performed for total proMMP 2 , proMMP 2 tissue inhibitor of metalloproteinase 2 ( TIMP 2 ) complex , and free active MMP 2 . ^^^ Interestingly , the tumor tissue expression of MMP 2 had an inverse correlation with proMMP 2 TIMP 2 complex levels in the serum . ^^^ This is further supported by the inverse correlation of the proMMP 2 TIMP 2 complex and MMP 2 tissue expression in the tumor . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
OBJECTIVES : To identify a relationship between clinical symptoms and matrix metalloproteinase ( MMP ) 2 and MMP 9 , tissue inhibitor of metalloproteinase ( TIMP ) 1 and TIMP 2 , and membrane type MMP 1 . ^^^ METHODS : Tumor samples from 232 patients with renal cell carcinoma with no distant metastasis were immunohistochemically stained for MMP 2 and MMP 9 , TIMP 1 and TIMP 2 , and membrane type MMP 1 . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Fatty streak , fibroatheroma with hemorrhage and calcification , and fully occluded lesions were enriched in MMP 2 , MMP 9 , and TIMP 1 and TIMP 2 , respectively . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
The objective of this study was to determine MMP 2 , MMP 7 , and MMP 9 and tissue inhibitors of metalloproteinases ( TIMP ) 1 and TIMP 2 expression in endometrial malignancies and their relation to clinical and histologic parameters . ^^^ MMP 2 expression increased and TIMP 2 expression fell as the histologic grade increased ( P = 0 . 0007 , P < 0 . 0001 , respectively ) . ^^^ MMP 2 expression correlated with lymph node metastasis ( P = 0 . 04 ) , while TIMP 2 expression correlated with the depth of myometrial invasion ( P = 0 . 01 ) , vasculolymphatic space involvement ( P = 0 . 02 ) , and lymph node metastasis ( P = 0 . 0003 ) . ^^^ High MMP 2 and low TIMP 2 expression were the most potent markers of endometrial tumors with a high risk of local and distant spread . . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
In cellular infiltrate we found expression of all the MMPs and TIMPs , the most distinct for MMP 1 , MMP 2 , MMP 10 and for TIMP 2 . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Furthermore , Ca2+ channel blockers specifically increased the proteolytic activity of the 72 kDa type 4 collagenase as shown by gelatin zymography and inhibited the transcription of tissue inhibitor of metalloproteinases 2 . . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Simian virus 40 transformation induced collagenase 1 gene expression ; in contrast , stromelysin 1 , 72 kDa gelatinase ( gelatinase A ) , tissue inhibitor of metalloproteinases 1 , and tissue inhibitor of metalloproteinases 2 genes were repressed . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
We have generated a C terminally truncated form of recombinant tissue inhibitor of metalloproteinases 2 ( designated rTIMP 2 delta ) in which the region of the inhibitor extending from residue 128 to 194 and including 3 of the 6 disulfide bonds is deleted . rTIMP 2 and rTIMP 2 delta had similar inhibitory activities toward interstitial collagenase and inhibited the activation of the precursor form of matrix metalloproteinase 2 ( proMMP 2 ) . rTIMP 2 also bound with high affinity ( Kd 0 . 99 nM ) to HT 1080 human fibrosarcoma cells treated with 12 O tetradecanoyl phorbol 13 acetate . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
This is true also , to a lesser extent , of cathepsin D , matrix metalloproteinase 2 combined with activators or tissue inhibitor of metalloproteinases 2 , CD 44 , E cadherin , p 53 and cripto . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Synthetic MMP inhibitors as well as recombinant tissue inhibitor of metalloproteinases 2 and 3 suppress the invasion of collagen gels and restore the capacity of C 7 caMEK1 cells to form cysts , thereby implicating the membrane type 1 MMP / MMP 2 proteolytic system in epithelial cell invasiveness and loss of multicellular organization . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
The chromatography of serum revealed a single peak at the position of about 90 kDa corresponding to an MMP 2 complexed with tissue inhibitor of metalloproteinases 2 . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
We previously reported that broad spectrum MMP inhibitors like GM 6001 enhance MT 1 MMP dependent activation of pro MMP 2 in the presence of tissue inhibitor of metalloproteinases 2 . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
Cruciate ligament metabolism was determined by measuring the following markers of collagen turnover : matrix metalloproteinase 2 ( MMP 2 ) , tissue inhibitor of metalloproteinases 2 , C terminal type 1 procollagen propeptide ( PICP ) , and the immature collagen derived crosslink dihydroxylysinonorleucine ( DHLNL ) . ^^^
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA
Interacting proteins: P16035 and P08253	Pubmed	SVM Score :0.0
NA