| Interacting proteins: P15918 and P09429 |
Pubmed |
SVM Score :0.0 |
| This complex includes the proteins RAG 1 , RAG 2 , HMG 1 or the closely related HMG 2 protein , and the components of the DNA dependent protein kinase . ^^^ |
|
| Interacting proteins: P15918 and P09429 |
Pubmed |
SVM Score :0.0 |
| In addition , HMG box containing proteins HMG 1 and HMG 2 enhance RAG 1 and RAG 2 mediated cleavage of substrates containing 23 RSS but not of substrates containing only 12 RSS . ^^^ |
|
| Interacting proteins: P15918 and P09429 |
Pubmed |
SVM Score :0.0 |
| Hence , it became obvious that the initial steps of the reaction are carried out by the lymphocyte specific proteins RAG 1 and RAG 2 ( recombination activating genes ) , with the help of members of the high mobility group protein family of DNA binding proteins , HMG 1 or HMG 2 . ^^^ |
|
| Interacting proteins: P15918 and P09429 |
Pubmed |
SVM Score :0.0 |
| The RAG 1 and RAG 2 proteins , as well as the DNA bending protein HMG 1 , are needed for efficient formation of this complex . ^^^ |
|
| Interacting proteins: P15918 and P09429 |
Pubmed |
SVM Score :0.0 |
| Under physiologic conditions , we found that the complex of RAG 1 , RAG 2 , and HMG 1 can successfully recapitulate the 12 / 23 rule with the same specificity as that seen intracellularly and in crude extracts . ^^^ |
|
| Interacting proteins: P15918 and P09429 |
Pubmed |
SVM Score :0.0 |
| The RAG 1 homeodomain recruits HMG 1 and HMG 2 to facilitate recombination signal sequence binding and to enhance the intrinsic DNA bending activity of RAG 1 RAG2 . 5 ( D ) J recombination is initiated by the specific binding of the RAG 1 RAG2 ( RAG1 / 2 ) complex to the heptamer nonamer recombination signal sequences ( RSS ) . ^^^ Here we show that the RAG 1 homeodomain directly interacts with both HMG boxes of HMG 1 and HMG 2 ( HMG 1 , 2 ) . ^^^ |
|
| Interacting proteins: P15918 and P09429 |
Pubmed |
SVM Score :0.0 |
| In addition to RAG 1 and RAG 2 , the protein HMG 1 is known to stimulate the efficiency of the cleavage reaction . ^^^ |
|
| Interacting proteins: P15918 and P09429 |
Pubmed |
SVM Score :0.0 |
| Here we report that the high mobility group protein , HMG 1 , is necessary for the correct nicking of the 23 bp recombination signal sequence ( 23 RSS ) by the recombination [ corrected ] activating gene ( RAG ) proteins , RAG 1 and RAG 2 . ^^^ |
|
| Interacting proteins: P15918 and P09429 |
Pubmed |
SVM Score :0.0 |
| All 39 5 ( H ) elements ( with their adjacent heptamer / nonamer signal ) were tested for site specific cleavage with purified human core RAG 1 and RAG 2 , and HMG 1 proteins in a 12 / 23 coupled cleavage reaction . ^^^ |
|
| Interacting proteins: P15918 and P09429 |
Pubmed |
SVM Score :0.0 |
| In contrast , 23 RSS complexes containing both RAG proteins and either HMG 1 or HMG 2 exhibited enhanced cleavage activity and displayed an altered distribution of cleavage products compared to 23 RSS complexes containing only RAG 1 and RAG 2 . ^^^ |
|
| Interacting proteins: P15918 and P09429 |
Pubmed |
SVM Score :0.0 |
| In the absence of HMG 1 , 1 found that two discrete RAG 1 / RAG 2 complexes are detected by mobility shift assay on all RSS substrates tested . ^^^ The addition of HMG 1 supershifts both complexes without altering the RAG protein stoichiometry . 1 find that 12 / 23 regulated recombination signal synapsis and cleavage are only supported in a protein DNA complex containing HMG 1 and a RAG 1 / RAG 2 tetramer . ^^^ Interestingly , the RAG 1 / RAG 2 tetramer also supports transposition , but HMG 1 is dispensable for its activity . . ^^^ |
|
| Interacting proteins: P15918 and P09429 |
Pubmed |
SVM Score :0.0 |
| Synapsis of recombination signal sequences located in cis and DNA underwinding in 5 ( D ) J recombination . 5 ( D ) J recombination requires binding and synapsis of a complementary ( 12 / 23 ) pair of recombination signal sequences ( RSSs ) by the RAG 1 and RAG 2 proteins , aided by a high mobility group protein , HMG 1 or HMG 2 . ^^^ |
|
| Interacting proteins: P15918 and P09429 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15918 and P09429 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15918 and P09429 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15918 and P09429 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15918 and P09429 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15918 and P09429 |
Pubmed |
SVM Score :0.0 |
| NA |
|