| Interacting proteins: P15884 and P35222 |
Pubmed |
SVM Score :0.53426917 |
| Hot spots in Tcf 4 for the interaction with beta catenin . 0.53426917^^^ |
|
| Interacting proteins: P15884 and P35222 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15884 and P35222 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15884 and P35222 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15884 and P35222 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15884 and P35222 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15884 and P35222 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15884 and P35222 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15884 and P35222 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15884 and P35222 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15884 and P35222 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15884 and P35222 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15884 and P35222 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15884 and P35222 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15884 and P35222 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15884 and P35222 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15884 and P35222 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15884 and P35222 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15884 and P35222 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15884 and P35222 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15884 and P35222 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15884 and P35222 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15884 and P35222 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15884 and P35222 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15884 and P35222 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15884 and P35222 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15884 and P35222 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15884 and P35222 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15884 and P35222 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15884 and P35222 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15884 and P35222 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15884 and P35222 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15884 and P35222 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15884 and P35222 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15884 and P35222 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15884 and P35222 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15884 and P35222 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15884 and P35222 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15884 and P35222 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15884 and P35222 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15884 and P35222 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15884 and P35222 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15884 and P35222 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15884 and P35222 |
Pubmed |
SVM Score :0.64436054 |
| Here we analyze the interaction between beta catenin and Tcf 4 and show that the N terminal 53 amino acids of Tcf 4 bind with high affinity to beta catenin . 0.64436054^^^ |
|
| Interacting proteins: P15884 and P35222 |
Pubmed |
SVM Score :0.51703966 |
| Phosphorylation of these residues does not modify the interaction of Tcf 4 with beta catenin but reduces its association to plakoglobin . 0.51703966^^^ |
|
| Interacting proteins: P15884 and P35222 |
Pubmed |
SVM Score :0.61347614 |
| Tcf 4 bound specifically to a glutathione S transferase ARDBD fusion protein and could be coimmunoprecipitated with beta catenin and transfected AR or endogenous AR in prostate cancer cells . 0.61347614^^^ |
|
| Interacting proteins: P15884 and P35222 |
Pubmed |
SVM Score :0.66064992 |
| Analysis of a 476 nucleotide segment of the 1490 nucleotide Myc genomic region upstream of the transcription start site demonstrated interactions between Tcf 4 and the Smad consensus binding region and associations of Smad 1 , beta catenin and Tcf 4 with oligo duplexes that encode the adjacent Tcf and Smad binding elements only in TgAlk3QD tissues . 0.66064992^^^ |
|
| Interacting proteins: P15884 and P35222 |
Pubmed |
SVM Score :0.58777283 |
| FGF 2 induced association of beta catenin with TCF 4 and up regulation of fibronectin in IBE cells , but not in KDFyn cells . 0.58777283^^^ |
|
| Interacting proteins: P15884 and P35222 |
Pubmed |
SVM Score :0.51101135 |
| We show that distinct residues of beta catenin are responsible for both binding and functional interactions with androgen receptor and with TCF 4 , thus allowing the introduction of missense mutations that selectively affect these interactions . beta Catenin and TIF2 / GRIP1 are each able to mediate binding between the other and androgen receptor in functional interactions that enhance ligand dependent transcription . 0.51101135^^^ |
|
| Interacting proteins: P15884 and P35222 |
Pubmed |
SVM Score :0.0 |
| Axin also suppressed Wnt 3a dependent activation of Tcf 4 which binds to beta catenin and acts as a transcription factor . ^^^ |
|
| Interacting proteins: P15884 and P35222 |
Pubmed |
SVM Score :0.0 |
| Phosphorylation of TCF 4 by NLK inhibited DNA binding by the beta catenin TCF 4 complex . ^^^ |
|
| Interacting proteins: P15884 and P35222 |
Pubmed |
SVM Score :0.0 |
| In addition , the human vimentin promoter was found to be up regulated by beta catenin and TCF 4 cotransfection . ^^^ |
|
| Interacting proteins: P15884 and P35222 |
Pubmed |
SVM Score :0.0 |
| Beta catenin and Tcf 4 are the downstream effectors of the Wnt signaling cascade . ^^^ |
|
| Interacting proteins: P15884 and P35222 |
Pubmed |
SVM Score :0.0 |
| We found that the gene for the basic helix loop helix transcription factor ITF 2 ( immunoglobulin transcription factor 2 ) was activated in rat E1A immortalized RK3E cells following neoplastic transformation by beta catenin or ligand induced activation of a beta catenin estrogen receptor fusion protein . ^^^ |
|
| Interacting proteins: P15884 and P35222 |
Pubmed |
SVM Score :0.0 |
| Using quantitative assays of gene expression , we found significantly elevated expression of the MMP 7 , CCND 1 ( Cyclin D 1 ) , CX 43 ( Connexin 43 ) , PPAR delta , and ITF 2 genes in OEAs with deregulated beta catenin . ^^^ Our findings indicate cyclin D 1 , MMP 7 , connexin 43 , PPAR delta , and ITF 2 , likely play important roles in the pathogenesis of those OEAs that manifest defects in beta catenin regulation . . ^^^ |
|
| Interacting proteins: P15884 and P35222 |
Pubmed |
SVM Score :0.0 |
| Upon translocation to the nucleus beta catenin serves as an activator of T cell factor ( Tcf ) dependent transcription leading to an increased expression of several specific target genes including c Myc , cyclin D 1 , MMP 7 , and ITF 2 . ^^^ |
|
| Interacting proteins: P15884 and P35222 |
Pubmed |
SVM Score :0.0 |
| The human T cell transcription factor 4 ( hTCF 4 ) interacts functionally with beta catenin in the Wnt signaling pathway , which regulates many developmental processes . ^^^ |
|
| Interacting proteins: P15884 and P35222 |
Pubmed |
SVM Score :0.0 |
| Beta catenin control of T cell transcription factor 4 ( Tcf 4 ) importation from the cytoplasm to the nucleus contributes to Tcf 4 mediated transcription in 293 cells . ^^^ In this study , we examined a constitutive active mutation , beta catenin ( T41A , S45A ) , for its potential as a nuclear import receptor for T cell transcription factor 4 in 293 cells . ^^^ |
|
| Interacting proteins: P15884 and P35222 |
Pubmed |
SVM Score :0.0 |
| Mutations of the genes encoding APC or beta catenin in colon carcinoma induce the constitutive formation of nuclear beta catenin / Tcf 4 complexes , resulting in activated transcription of Tcf target genes . ^^^ |
|
| Interacting proteins: P15884 and P35222 |
Pubmed |
SVM Score :0.0 |
| Mutations of APC cause aberrant accumulation of beta catenin , which then binds T cell factor 4 ( Tcf 4 ) , causing increased transcriptional activation of unknown genes . ^^^ Expression of c MYC was shown to be repressed by wild type APC and activated by beta catenin , and these effects were mediated through Tcf 4 binding sites in the c MYC promoter . ^^^ |
|
| Interacting proteins: P15884 and P35222 |
Pubmed |
SVM Score :0.0 |
| TCF 4 binds beta catenin and is expressed in distinct regions of the embryonic brain and limbs . ^^^ Tcf 4 expressing cells showed nuclear localization of beta catenin . ^^^ |
|
| Interacting proteins: P15884 and P35222 |
Pubmed |
SVM Score :0.0 |
| Tcf 4 is a member of the Tcf / Lef family of transcription factors that interact functionally with beta catenin to mediate Wnt signaling in vertebrates . ^^^ We have previously demonstrated that the tumor suppressor function of APC in the small intestine is mediated via regulation of Tcf 4 / beta catenin transcriptional activity . ^^^ |
|
| Interacting proteins: P15884 and P35222 |
Pubmed |
SVM Score :0.0 |
| The matrilysin promoter is upregulated as much as 12 fold by beta catenin in colon tumor cell lines in a manner inversely proportional to the endogenous levels of beta catenin / Tcf complex and is dependent upon a single optimal Tcf 4 recognition site . ^^^ |
|
| Interacting proteins: P15884 and P35222 |
Pubmed |
SVM Score :0.0 |
| Synergy between tumor suppressor APC and the beta catenin Tcf 4 target Tcf 1 . ^^^ Mutations in APC or beta catenin inappropriately activate the transcription factor Tcf 4 , thereby transforming intestinal epithelial cells . ^^^ Tcf 1 may act as a feedback repressor of beta catenin Tcf 4 target genes and thus may cooperate with APC to suppress malignant transformation of epithelial cells . . ^^^ |
|
| Interacting proteins: P15884 and P35222 |
Pubmed |
SVM Score :0.0 |
| Moreover , although LEF 1 and TCF 4 synergize with beta catenin and plakoglobin to activate OT , both suppress the signaling activity of cnxPg . ^^^ |
|
| Interacting proteins: P15884 and P35222 |
Pubmed |
SVM Score :0.0 |
| This leads to accumulation of beta catenin , which together with the DNA binding protein TCF 4 functions as a transcriptional activator . ^^^ Here we report the identification of the matrix metalloproteinase MMP 7 as another target gene of beta catenin / TCF 4 . ^^^ |
|
| Interacting proteins: P15884 and P35222 |
Pubmed |
SVM Score :0.0 |
| In APC deficient colon carcinoma cell lines , beta catenin accumulates and is constitutively complexed with nuclear Tcf 4 . ^^^ A proportion of APC wild type colon carcinomas and melanomas also contains constitutive nuclear Tcf 4 / beta catenin complexes as a result of dominant mutations in the N terminus of beta catenin that render it insensitive to downregulation by APC , GSK 3 beta , and Axin / Conductin . ^^^ Based on the established role for Tcf 4 in maintaining intestinal stem cells it is likely that deregulation of c myc expression as a result of constitutive Tcf 4 / beta catenin activity promotes uncontrolled intestinal cell proliferation . ^^^ |
|
| Interacting proteins: P15884 and P35222 |
Pubmed |
SVM Score :0.0 |
| Rod formation was prevented by coexpression of N cadherin , APC , and Tcf 4 , which bind to the armadillo repeats of beta catenin , but not by coexpression of alpha catenin , although alpha catenin expression did prevent accumulation of beta catenin in the nucleus . ^^^ Interestingly , when alpha catenin , beta catenin , and Tcf 4 were coexpressed they colocalized in the nucleus , and this correlated with a decrease in beta catenin / Tcf dependent transcriptional activity . ^^^ These results indicate that binding of beta catenin to Tcf 4 overrides the function of alpha catenin to sequester beta catenin in the cytoplasm and suggest that alpha catenin can regulate beta catenin signaling in the nucleus . . ^^^ |
|
| Interacting proteins: P15884 and P35222 |
Pubmed |
SVM Score :0.0 |
| This leads to the accumulation of nuclear beta catenin , which , together with the DNA binding protein TCF 4 , functions as a transcriptional activator . ^^^ |
|
| Interacting proteins: P15884 and P35222 |
Pubmed |
SVM Score :0.0 |
| Here , we identify a novel beta catenin interacting protein , ICAT , that was found to inhibit the interaction of beta catenin with TCF 4 and represses beta catenin TCF 4 mediated transactivation . ^^^ |
|
| Interacting proteins: P15884 and P35222 |
Pubmed |
SVM Score :0.0 |
| These differences may be attributable to an enhanced affinity of S37A beta catenin for LEF 1 and TCF 4 , as observed here by immunoprecipitation assays . ^^^ |
|
| Interacting proteins: P15884 and P35222 |
Pubmed |
SVM Score :0.0 |
| Potential invasion genes regulated by beta catenin and its DNA binding partner TCF 4 were identified by a computer search for the consensus DNA binding sequence in relevant promoter regions . ^^^ |
|
| Interacting proteins: P15884 and P35222 |
Pubmed |
SVM Score :0.0 |
| We assessed the importance of mutations in the regulatory domain , located within exon 3 of CTNNB 1 , in 103 rectal carcinomas and correlated these data with presence of microsatellite instability , somatic frame shift alterations of the TCF 4 gene , and APC gene mutations in the tumors . ^^^ |
|
| Interacting proteins: P15884 and P35222 |
Pubmed |
SVM Score :0.0 |
| Incubation of cell extracts with active DNA fragments containing the sequence CAAAG caused retardation of their mobilities on polyacrylamide gels , and Western blotting identified Tcf 4 , beta catenin , and E cadherin in the relevant DNA complexes in vitro . ^^^ |
|
| Interacting proteins: P15884 and P35222 |
Pubmed |
SVM Score :0.0 |
| Accordingly , 1alpha , 25 ( OH ) 2D ( 3 ) repressed beta catenin TCF 4 transcriptional activity . ^^^ Moreover , VDR activity was enhanced by ectopic beta catenin and reduced by TCF 4 . ^^^ Also , 1alpha , 25 ( OH ) 2D ( 3 ) inhibited expression of beta catenin TCF 4 responsive genes , c myc , peroxisome proliferator activated receptor delta , Tcf 1 , and CD 44 , whereas it induced expression of ZO 1 . ^^^ Our results show that 1alpha , 25 ( OH ) 2D ( 3 ) induces E cadherin and modulates beta catenin TCF 4 target genes in a manner opposite to that of beta catenin , promoting the differentiation of colon carcinoma cells . . ^^^ |
|
| Interacting proteins: P15884 and P35222 |
Pubmed |
SVM Score :0.0 |
| To investigate how PS 1 might regulate beta catenin signaling , we determined whether PS 1 interacts with other elements of the beta catenin signaling cascade , such as the Tcf 4 transcription factor . ^^^ |
|
| Interacting proteins: P15884 and P35222 |
Pubmed |
SVM Score :0.0 |
| A fragment of beta catenin and a peptide encoding the NH 2 terminus of Tcf 4 that block the interaction between beta catenin and Tcf 3 stimulate beta catenin degradation , indicating this interaction normally plays an important role in regulating beta catenin turnover . ^^^ |
|
| Interacting proteins: P15884 and P35222 |
Pubmed |
SVM Score :0.0 |
| Tcf 4 can specifically recognize beta catenin using alternative conformations . ^^^ Because Tcf 4 is the predominant Tcf factor present in colon cancer cells , drugs that specifically disrupt the beta catenin Tcf 4 complex could be useful in treating colon cancers . ^^^ Here we report the crystal structure of a beta catenin Tcf 4 complex at 2 . 0 A resolution . ^^^ Our structural and mutagenesis studies show that Tcf 4 docks specifically to beta catenin using several distinct conformations in its essential central region . ^^^ These conformations allow different glutamate residues in the central region of Tcf 4 to form a salt bridge with the same critical charged button , Lys 312 of beta catenin . ^^^ |
|
| Interacting proteins: P15884 and P35222 |
Pubmed |
SVM Score :0.0 |
| In order to assess the status of the Wnt signaling components in CRC with MSI , mutational analyses of the beta catenin , APC , Axin 1 , and T cell factor 4 ( TCF 4 ) genes were performed . ^^^ |
|
| Interacting proteins: P15884 and P35222 |
Pubmed |
SVM Score :0.0 |
| LEF 1 beta cat transactivation in vitro was also repressed by inhibitor of beta catenin and Tcf 4 ( ICAT ) , a physiological inhibitor of Wnt / Wg signaling that interacts with ARM repeats 11 and 12 , and by the nonsteroidal anti inflammatory compound , sulindac . ^^^ |
|
| Interacting proteins: P15884 and P35222 |
Pubmed |
SVM Score :0.0 |
| Electrophoresis mobility shift assay demonstrated that TCF 4 and beta catenin form a complex and have DNA binding activity . ^^^ |
|
| Interacting proteins: P15884 and P35222 |
Pubmed |
SVM Score :0.0 |
| Finally , genetic aberrations of several components of the beta catenin pathways , eg , Frizzled ( Frz ) , AXIN , and TCF 4 , may potentially contribute to colorectal carcinogenesis . ^^^ |
|
| Interacting proteins: P15884 and P35222 |
Pubmed |
SVM Score :0.0 |
| The frizzled 2 receptor , Apc , beta catenin , groucho , and a novel isoform of TCF 4 ( officially named Tcf7l2 ) were identified in developing pituitary libraries . ^^^ |
|
| Interacting proteins: P15884 and P35222 |
Pubmed |
SVM Score :0.0 |
| To test if Tcf was necessary to confer beta catenin induced survival , loss of function studies were carried out with a dominant negative Tcf 4 transgene lacking the beta catenin binding domain , Tcf 4 ( N 31 ) . ^^^ Indeed , loss of Tcf 4 activity abolished beta catenin induced survival . ^^^ We further postulated that beta catenin and Tcf promoted cell cycle progression by activating cyclin D 1 , a target gene of Tcf 4 . ^^^ Beta catenin activated cyclin D 1 , and this activation was partially blocked with loss of Tcf 4 . ^^^ In conclusion , beta catenin and Tcf 4 play a dual role in vascular remodeling by inhibiting VSMC apoptosis and promoting proliferation . . ^^^ |
|
| Interacting proteins: P15884 and P35222 |
Pubmed |
SVM Score :0.0 |
| Frequent alterations of the beta catenin and TCF 4 genes , but not of the APC gene , in colon cancers with high frequency microsatellite instability . ^^^ To better understand the molecular characteristics of colon cancers with MSI H , we analyzed these cancers for alterations of genes , such as APC , beta catenin , and TCF 4 genes , involved in the Wnt signaling pathway . ^^^ In total , 78 % of MSI H tumors and 84 % of the remaining tumors had at least one alteration in APC , beta catenin , or the TCF 4 genes . ^^^ |
|
| Interacting proteins: P15884 and P35222 |
Pubmed |
SVM Score :0.0 |
| Not only did expression of CLDN 1 decrease significantly in response to reduction of intracellular beta catenin by adenovirus mediated transfer of wild type APC into the APC deficient colon cancer cells , but also two putative Tcf 4 binding elements in the 5 ' flanking region of CLDN 1 were confirmed to be responsible for activating its transcription . ^^^ |
|
| Interacting proteins: P15884 and P35222 |
Pubmed |
SVM Score :0.0 |
| TCF 4 and beta catenin form a transcription complex , which is important for both maintenance of normal epithelium and development of colorectal tumors . ^^^ |
|
| Interacting proteins: P15884 and P35222 |
Pubmed |
SVM Score :0.0 |
| We have recently identified ICAT , a beta catenin interacting protein that interferes with the interaction between beta catenin and TCF 4 , thereby negatively regulating Wnt signaling . ^^^ |
|
| Interacting proteins: P15884 and P35222 |
Pubmed |
SVM Score :0.0 |
| We investigated 37 malignant melanomas ( 15 primary tumors and 22 metastases ) for alterations of 4 genes encoding members of this pathway , i . e . , CTNNB 1 ( beta catenin gene , 3p22 . 1 ) , APC ( adenomatous polyposis coli gene , 5q22 . 2 ) , BTRC ( beta transducin repeat containing protein gene , 10q24 . 3 ) and ICAT ( inhibitor of beta catenin and Tcf 4 , 1p36 . 2 ) . ^^^ |
|
| Interacting proteins: P15884 and P35222 |
Pubmed |
SVM Score :0.0 |
| Although activation of Wnt signaling by inhibition of GSK 3 activity or ectopic expression of dominant stable beta catenin blocks apoptosis , inhibition of Wnt signaling through expression of dominant negative TCF 4 increases apoptosis . ^^^ |
|
| Interacting proteins: P15884 and P35222 |
Pubmed |
SVM Score :0.0 |
| Loss of E cadherin beta catenin adhesion is an important step in the progression of many epithelial malignancies . beta catenin plays also a role in intracellular signaling and can function as an oncogene when binds to the T cell factor 4 ( Tcf 4 ) binding site in the promotor region of cyclin D 1 and transactivates genes after translocation to the nucleus . ^^^ |
|
| Interacting proteins: P15884 and P35222 |
Pubmed |
SVM Score :0.0 |
| However , addition of 20 microM H ( 2 ) O ( 2 ) directly to HEK 293 cells transiently transfected with wild type or mutant beta catenin constructs and TCF 4 had no significant effect on beta catenin / TCF 4 reporter activity or beta catenin expression levels . ^^^ In contrast , 2 25 microM EGCG inhibited beta catenin / TCF 4 reporter activity in a concentration dependent fashion and there was a concomitant reduction in beta catenin protein levels in the cell lysates without changes in TCF 4 expression . ^^^ The results indicate that physiologically relevant concentrations of tea and EGCG inhibit beta catenin / TCF 4 reporter activity in HEK 293 cells due to reduced expression of beta catenin and that this is unlikely to be an artifact of H ( 2 ) O ( 2 ) generation under the assay conditions used here . ^^^ |
|
| Interacting proteins: P15884 and P35222 |
Pubmed |
SVM Score :0.0 |
| This possibility was supported by the finding that a dominant negative mutant of the transcription factor TCF 4 , designed to inhibit beta catenin signaling , also inhibited HMEC 1 cell growth . ^^^ |
|
| Interacting proteins: P15884 and P35222 |
Pubmed |
SVM Score :0.0 |
| Gel shift assays revealed binding of Tcf 4 as the only Lef / Tcf family member and of beta catenin to the Lef / Tcf site in the cyclin D 1 promoter . ^^^ |
|
| Interacting proteins: P15884 and P35222 |
Pubmed |
SVM Score :0.0 |
| In the normal looking intestinal mucosa of Apc ( min ) and A 33 ( delta N beta cat ) mice , white tea plus sulindac treatment markedly attenuated the expression of beta catenin protein , and this was recapitulated in vitro in cells transiently transfected with beta catenin plus Tcf 4 and treated with tea or the major tea polyphenol epigallocatechin 3 gallate ( EGCG ) . ^^^ |
|
| Interacting proteins: P15884 and P35222 |
Pubmed |
SVM Score :0.0 |
| The key components of this pathway , beta catenin and its partner TCF 4 / LEF 1 , exert their effects on transcription by entering the nuclei , where they associate with the TCF 4 / LEF 1 DNA motif positioned in the promoters of several important genes . ^^^ A mutant variant of TCF 4 with no binding site for beta catenin was able to down regulate LTR transcription , suggesting that beta catenin may not be directly involved in the observed regulatory events . ^^^ |
|
| Interacting proteins: P15884 and P35222 |
Pubmed |
SVM Score :0.0 |
| A reporter gene assay using the 5 ' flanking region of APCDD 1 indicated that transfection of beta catenin together with wild type Tcf 4 into HeLa cells increased the reporter activity through two putative Tcf / lymphoid enhancer factor binding motifs upstream of the transcription start site , indicating that APCDD 1 is one of the direct targets of this transcription complex . ^^^ |
|
| Interacting proteins: P15884 and P35222 |
Pubmed |
SVM Score :0.0 |
| The activation of the pathway was mainly due to the mutation of adenomatous polyposis coli ( APC ) or beta catenin , and Tcf 4 was highly expressed in these cell lines with upregulated signaling . ^^^ |
|
| Interacting proteins: P15884 and P35222 |
Pubmed |
SVM Score :0.0 |
| Given the recent work identifying an AR / beta catenin interaction , and from our finding that liganded AR does not prompt gross changes in the constitutive nuclear localization of TCF 4 or mutant beta catenin , we hypothesized that transcription factor ( i . e . ^^^ Interestingly , TCF 4 over expression potently antagonizes AR function ; however , this inhibition may occur independently of beta catenin / TCF4 interaction . ^^^ These results from TCF 4 over expression analyses , taken together , provide further evidence that AR mediated suppression of CRT is a consequence of limiting amounts of beta catenin , and not AR target gene expression . ^^^ |
|
| Interacting proteins: P15884 and P35222 |
Pubmed |
SVM Score :0.0 |
| Loss of functional APC protein activates the Wnt signal transduction pathway , allowing the nuclear accumulation of beta catenin , which then binds to T cell factor 4 ( Tcf 4 ) , causing increased transcriptional activation of downstream target genes . ^^^ Mutant beta catenin expression up regulated the COX 2 promoter activity and the endogenous COX 2 mRNA expression in HuH 7 , hepatocellular carcinoma cell line , which is partially abrogated by cotransfection with a dominant negative Tcf 4 expression vector . ^^^ |
|
| Interacting proteins: P15884 and P35222 |
Pubmed |
SVM Score :0.0 |
| ICAT , an inhibitor of T cell factor 4 ( TCF 4 ) , and E cadherin binding to beta catenin also blocked binding of the androgen receptor LBD . ^^^ We also demonstrated cross talk between the WNT and androgen receptor signaling pathways because excess androgen receptor could interfere with WNT signaling and excess TCF 4 inhibited the interaction of beta catenin and androgen receptor . ^^^ |
|
| Interacting proteins: P15884 and P35222 |
Pubmed |
SVM Score :0.0 |
| When TCF 4 was bound to this element , TGF beta dissociated beta catenin and repressed the transcriptional activity of the c myc promoter . ^^^ However , TGF beta could not dissociate beta catenin and could not repress c myc transcription when LEF 1 was bound to the element instead of TCF 4 . ^^^ |
|
| Interacting proteins: P15884 and P35222 |
Pubmed |
SVM Score :0.0 |
| Moreover , messenger RNA of beta catenin and Tcf 4 , but not GSK 3beta , was found to be overexpressed in HCC . ^^^ On analyzing the relationship between alterations of beta catenin or Tcf 4 and C myc or Cyclin D 1 expression , we found that mutations of beta catenin , as well as overexpression of beta catenin or the Tcf 4 gene were independently correlated with C myc gene overexpression in HCC . ^^^ CONCLUSION : Our present findings strongly suggest that mutations of beta catenin , as well as overexpression of beta catenin and the Tcf 4 gene , independently activate the Wnt pathway in HCC , with the target gene most likely to be C myc . . ^^^ |
|
| Interacting proteins: P15884 and P35222 |
Pubmed |
SVM Score :0.0 |
| In this study , we aim to investigate the expression pattern of various components of the Wnt pathway including b catenin and its partners LEF 1 / TCF 4 , GSK 3beta and their nuclear target genes such as c myc and cyclin D 1 during mouse brain development . ^^^ |
|
| Interacting proteins: P15884 and P35222 |
Pubmed |
SVM Score :0.0 |
| Sumoylation is involved in beta catenin dependent activation of Tcf 4 . ^^^ Although PIASy did not affect the interaction of Tcf 4 with beta catenin or DNA , Tcf 4 , SUMO 1 and PIASy were co localized in the nucleus and present in a complex in the PML body . ^^^ PIASy enhanced beta catenin dependent transcriptional activity of Tcf 4 , whereas Axam inhibited it . ^^^ Furthermore , beta catenin and PIASy activated Tcf 4 ( K297R ) , in which Lys 297 was changed to arginine , less than wild type Tcf 4 . ^^^ These results suggest that sumoylation of Tcf 4 is involved in beta catenin dependent and Tcf 4 mediated gene expression in the Wnt signaling pathway . . ^^^ |
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| Interacting proteins: P15884 and P35222 |
Pubmed |
SVM Score :0.0 |
| The human T cell factor 4 gene splicing isoforms , Wnt signal pathway , and apoptosis in renal cell carcinoma . beta Catenin and transcriptional factor TCF 4 ( human T cell factor 4 ) genes comprise the Wnt signal . ^^^ We hypothesize that the beta catenin and TCF 4 gene and Wnt signal are important in the progression of renal cell carcinoma ( RCC ) . ^^^ To test this hypothesis , we investigated TCF 4 splicing isoforms , beta catenin , and Wnt signal pathway ( cyclin D 1 , c myc , c jun , and MMP 7 ) in three RCC cell lines ( A 498 , Caki 1 , and Caki 2 ) , 38 primary RCCs , and 29 normal kidney samples . ^^^ In samples in which beta catenin was not overexpressed , the target genes of Wnt signal were regulated through TCF 4 splicing isoforms . ^^^ |
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| Interacting proteins: P15884 and P35222 |
Pubmed |
SVM Score :0.0 |
| T cell factor 4N ( TCF 4N ) , a novel isoform of mouse TCF 4 , synergizes with beta catenin to coactivate C / EBPalpha and steroidogenic factor 1 transcription factors . ^^^ |
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| Interacting proteins: P15884 and P35222 |
Pubmed |
SVM Score :0.0 |
| A key component of the pathway is beta catenin that , in association with TCF 4 , directly regulates the expression of Wnt responsive genes . ^^^ |
|
| Interacting proteins: P15884 and P35222 |
Pubmed |
SVM Score :0.0 |
| APC 3 10 15 beta catenin binding domain potentiates beta catenin association to TBP and upregulates TCF 4 transcriptional activity . ^^^ Association of this fragment prevents the interaction of beta catenin with E cadherin but not with TCF 4 . ^^^ Transfection of this fragment to several cell lines increases the transcriptional activity of the beta catenin TCF 4 complex and promotes the translocation of beta catenin to the nucleus . ^^^ Therefore , APC 3 10 15 domain plays a positive role in the control of transcriptional activity of beta catenin TCF 4 and can contribute to explain the role of the truncated forms of APC in colon tumorigenesis . . ^^^ |
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| Interacting proteins: P15884 and P35222 |
Pubmed |
SVM Score :0.0 |
| We investigated the mRNA expression of beta catenin and Tcf 4 in N , T and M in 12 cell lines and in tissues samples of 14 patients . ^^^ |
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| Interacting proteins: P15884 and P35222 |
Pubmed |
SVM Score :0.0 |
| We determined Beta catenin dependent TCF 4 transcriptional activity by measuring the activity of the luciferase gene placed under the control of TCF 4 regulatory sequences . ^^^ The IC 50 values for Beta catenin / TCF 4 signaling inhibition by NO ASA were : o , 2 . 6 + / 0 . 4 ; m , 15 + / 5 ; p , 1 . 1 + / 0 . 1 microM ; and for ASA , > 5 , 000 microM . ^^^ NO ASA disrupted the association of Beta catenin and TCF 4 in the nucleus , whereas ASA did not affect it . ^^^ Thus , NO ASA inhibits Beta catenin mediated TCF activity by preventing the formation of the Beta catenin / TCF 4 complex . ^^^ |
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| Interacting proteins: P15884 and P35222 |
Pubmed |
SVM Score :0.0 |
| Upregulation of Wnt 1 resulted in beta catenin mediated activation of Tcf 4 , leading to increased beta TrCP 1 expression and NF kappaB activity . ^^^ Furthermore , VSMCs harboring a Tcf 4 mutant lacking a beta catenin binding domain exhibited a significant reduction in beta TrCP 1 expression along with abolishment of NF kappaB activity . ^^^ |
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| Interacting proteins: P15884 and P35222 |
Pubmed |
SVM Score :0.0 |
| This is consistent with our earlier finding that inhibition of the beta catenin : T cell factor 4 ( Tcf 4 ) pathway by the adenomatous polyposis coli protein down regulates survivin expression and with recent evidence that sulindac induces beta catenin degradation , which would reduce Tcf 4 activation . ^^^ This suggests that the beta catenin : Tcf 4 : survivin mechanism may be a useful target for therapy or chemoprevention of colon cancer . . ^^^ |
|
| Interacting proteins: P15884 and P35222 |
Pubmed |
SVM Score :0.0 |
| We reconstructed a protein protein network system of human beta catenin and TCF 4 , in Saccharomyces cerevisiae . beta Catenin and TCF 4 proteins form a complex and transactivate reporter genes . ^^^ Co expressed wild type APC with beta catenin and TCF 4 inhibit the transcriptional activity of the beta catenin / TCF4 complex in yeast , as well as in mammals . ^^^ |
|
| Interacting proteins: P15884 and P35222 |
Pubmed |
SVM Score :0.0 |
| Expression of BAMBI in colorectal tumor cell lines was repressed by a dominant negative mutant of TCF 4 or by an inhibitor of beta catenin TCF interaction , suggesting that beta catenin is responsible for the aberrant expression of BAMBI in colorectal tumor cells . ^^^ |
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| Interacting proteins: P15884 and P35222 |
Pubmed |
SVM Score :0.0 |
| Alterations of beta catenin and Tcf 4 instead of GSK 3beta contribute to activation of Wnt pathway in hepatocellular carcinoma . ^^^ METHODS : We analyzed the alterations of three key components of Wnt pathway , beta catenin , glycogen synthase kinase 3beta ( GSK 3beta ) and T cell factor 4 ( Tcf 4 ) , in 34 samples of hepatocellular carcinoma ( HCC ) and paracancerous normal liver by immunohistochemistry , polymerase chain reaction single strand conformation polymorphism ( PCR SSCP ) , direct sequencing , semi quantitative reverse transcription polymerase chain reaction ( RT PCR ) and in situ hybridization . ^^^ Moreover , mRNA of beta catenin and Tcf 4 but not GSK 3beta was found to be over expressed in HCCs . ^^^ On analyzing the relationship between alterations of beta catenin or Tcf 4 and C myc or Cyclin D 1 expression , we found that the mutations of beta catenin as well as over expression of beta catenin or Tcf 4 gene were independently correlated with C myc gene over expression in HCCs . ^^^ CONCLUSIONS : Our present findings strongly suggest mutations of beta catenin as well as over expression of beta catenin and Tcf 4 gene activate the Wnt pathway in HCC independently with the target gene most likely to be C myc . . ^^^ |
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| Interacting proteins: P15884 and P35222 |
Pubmed |
SVM Score :0.0 |
| In the search for efficient treatment and prevention of malignancies associated with increased levels of cytoplasmic beta catenin , we created chimeric F box fusion proteins by replacing the WD 40 repeat of beta TrCP with the beta catenin binding domains of Tcf 4 and E cadherin . ^^^ |
|
| Interacting proteins: P15884 and P35222 |
Pubmed |
SVM Score :0.0 |
| To identify compounds that inhibit association between Tcf 4 and beta catenin , we screened libraries of natural compounds in a high throughput assay for immunoenzymatic detection of the protein protein interaction . ^^^ |
|
| Interacting proteins: P15884 and P35222 |
Pubmed |
SVM Score :0.0 |
| We hypothesized that LRP 6 is a critical mediator governing the regulation of the canonical Wnt / beta catenin / T cell factor 4 ( Tcf 4 ) cascade in the vasculature . ^^^ This hypothesis was based on our previous work demonstrating a role for the beta catenin / Tcf 4 pathway in vascular remodeling as well as work in other cell systems establishing a role for LRP family members in the Wnt cascade . ^^^ LRP 6 induced stimulation of Tcf was blocked in VSMCs harboring constitutive expression of a dominant negative Tcf 4 transgene lacking the beta catenin binding domain , suggesting that LRP 6 induced activation of Tcf was mediated through a beta catenin dependent signal . ^^^ |
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| Interacting proteins: P15884 and P35222 |
Pubmed |
SVM Score :0.0 |
| Here , we show that the transcription factor activator protein ( AP ) 2alpha inhibited a beta catenin / TCF responsive reporter in human embryonic kidney 293 cells and in two human colorectal cancer lines , despite the fact that beta catenin and TCF 4 protein levels were unchanged in the nucleus . ^^^ Co immunoprecipitation studies revealed that AP 2alpha formed a complex with APC and beta catenin and that AP 2alpha disrupted beta catenin / TCF 4 interactions in the nucleus . ^^^ |
|
| Interacting proteins: P15884 and P35222 |
Pubmed |
SVM Score :0.0 |
| Associated Wnt signaling components , including low density lipoprotein receptor related protein 5 ( LRP 5 ) , kremen 1 , dickkopf 1 ( Dkk 1 ) , secreted Frizzled related peptide ( sFRP ) 2 , sFRP 3 , sFRP 4 , Disheveled ( Dvl ) , GSK 3beta , adenomatous polyposis coli ( APC ) , beta catenin , T cell factor ( TCF ) 1 , and TCF 4 , were also identified . ^^^ |
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| Interacting proteins: P15884 and P35222 |
Pubmed |
SVM Score :0.0 |
| Gal 3 binds to beta catenin / Tcf complex , colocalizes with beta catenin in the nucleus , and induces the transcriptional activity of Tcf 4 as determined by the TOP / FOPFLASH reporter system . ^^^ |
|
| Interacting proteins: P15884 and P35222 |
Pubmed |
SVM Score :0.0 |
| This induction was completely blocked by the expression of dominant negative TCF 4 , suggesting that beta catenin and TCF 4 complex formation is required for Rac 1 mediated transcription . ^^^ We observed that Rac 1 co immunoprecipitates with beta catenin and TCF 4 only in its active GTP bound form . ^^^ |
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| Interacting proteins: P15884 and P35222 |
Pubmed |
SVM Score :0.0 |
| However , they associate to distinct Tcf 4 subdomains causing opposing effects on Tcf 4 binding to DNA : whereas beta catenin does not affect this binding , plakoglobin prevents it . ^^^ Interaction of Tcf 4 , as well as other desmosomal or adherens junction components , with beta catenin or plakoglobin takes place through the central armadillo domain . ^^^ Moreover the specificity of the interaction of beta catenin and plakoglobin with different subdomains in Tcf 4 or with other junctional components resides within the terminal tails and not in the armadillo domain . ^^^ |
|
| Interacting proteins: P15884 and P35222 |
Pubmed |
SVM Score :0.0 |
| Gene and protein expression analysis revealed that the members downstream of Wnt in this signaling pathway including beta catenin , GSK 3beta , Axin , and TCF 4 were significantly down regulated as ES cells differentiated into hematopoietic progenitors . ^^^ |
|
| Interacting proteins: P15884 and P35222 |
Pubmed |
SVM Score :0.0 |
| TCF 4 mediates cell type specific regulation of proglucagon gene expression by beta catenin and glycogen synthase kinase 3beta . ^^^ Furthermore , both TCF 4 and beta catenin bind to the glu gene promoter , as detected by chromatin immunoprecipitation . ^^^ |
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| Interacting proteins: P15884 and P35222 |
Pubmed |
SVM Score :0.0 |
| A specific DNA element within the EDN 1 promoter is required for activation , and is associated with beta catenin ' s cognate DNA binding partner , TCF 4 , in vivo . ^^^ |
|
| Interacting proteins: P15884 and P35222 |
Pubmed |
SVM Score :0.0 |
| Systematic peptide array based delineation of the differential beta catenin interaction with Tcf 4 , E cadherin , and adenomatous polyposis coli . ^^^ To identify selective beta catenin binding hot spots of Tcf 4 , E cadherin , and APC , array technology with peptides of up to 53 amino acids length was used . ^^^ |
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| Interacting proteins: P15884 and P35222 |
Pubmed |
SVM Score :0.0 |
| We show that activation of canonical signaling by Wnt 1 or ectopic expression of active beta catenin , TCF 4 or LRP 6 mutants induces transcription of the human DKK 1 gene . ^^^ |
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| Interacting proteins: P15884 and P35222 |
Pubmed |
SVM Score :0.0 |
| The ERK pathway was however , activated by beta catenin transfection , which was abolished by co transfection with dominant negative Tcf 4 . ^^^ Therefore , ERK pathway activation by Wnt signaling could occur at multiple levels , including beta catenin independent direct signaling resulting from a Wnt3a and beta catenin / Tcf 4 dependent post gene transcriptional event . ^^^ |
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| Interacting proteins: P15884 and P35222 |
Pubmed |
SVM Score :0.0 |
| Electrophoretic mobility shift and supershift assays revealed specific binding of human TCF 4 and beta catenin to oligonucleotides corresponding to a potential TCF binding site in the versican promoter . ^^^ Co transfection of TCF 4 with versican Luc did not increase promoter activity , but addition of beta catenin and TCF 4 significantly stimulated basal versican promoter activity . ^^^ |
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| Interacting proteins: P15884 and P35222 |
Pubmed |
SVM Score :0.0 |
| Immunoprecipitation analysis also showed that the binding of beta catenin to Tcf 4 was also disrupted by quercetin . ^^^ Western blot analysis proved these decreased bindings resulted from decreased level of beta catenin and Tcf 4 product in nucleus caused by quercetin . ^^^ Together , we suggest that quercetin is an excellent inhibitor of beta catenin / Tcf signaling in SW 480 cell lines , and the reduced beta catenin / Tcf transcriptional activity is due to the decreased nuclear beta catenin and Tcf 4 proteins . . ^^^ |
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| Interacting proteins: P15884 and P35222 |
Pubmed |
SVM Score :0.0 |
| Inhibition of beta catenin / Tcf signaling by ectopic expression of dominant negative Tcf 4 resulted in significant inhibition of growth in squamous cell carcinoma cells . ^^^ |
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| Interacting proteins: P15884 and P35222 |
Pubmed |
SVM Score :0.0 |
| An unselected series of 310 colorectal carcinomas , stratified according to microsatellite instability ( MSI ) and DNA ploidy , was examined for mutations and / or promoter hypermethylation of five components of the WNT signaling cascade [ APC , CTNNB 1 ( encoding beta catenin ) , AXIN 2 , TCF 4 , and WISP 3 ] and three genes indirectly affecting this pathway [ CDH 1 ( encoding E cadherin ) , PTEN , and TP 53 ] . ^^^ |
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| Interacting proteins: P15884 and P35222 |
Pubmed |
SVM Score :0.0 |
| Upregulation of TCF 4 expression as a transcriptional target of beta catenin / p300 complexes during trans differentiation of endometrial carcinoma cells . ^^^ Our previous study indicated that nuclear beta catenin accumulation provides an initial signal for trans differentiation toward the squamoid phenotype of endometrial carcinoma ( Em Ca ) cells in a TCF 4 dependent manner , which makes this a possible factor for a positive prognosis . ^^^ We show here that beta catenin can directly induce transcription from the TCF 4 promoter , the effect being enhanced by the p 300 coactivator . ^^^ In clinical cases , nuclear beta catenin accumulation was found to frequently overlap with TCF 4 immunoreactivity in morules and surrounding glandular carcinoma lesions , showing a significant positive correlation ( r = 0 . 82 , P < 0 . 0001 ) , in contrast to areas of squamous metaplasia ( SqM ) within Em Cas . ^^^ In cases with coexistence of two squamoid features in trans differentiated areas , loss of nuclear beta catenin and TCF 4 immunoreactivity was closely related to change in the morphology from the morular to the SqM phenotype . ^^^ |
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| Interacting proteins: P15884 and P35222 |
Pubmed |
SVM Score :0.0 |
| Western blot analysis suggested that no change in the amount of cytosolic and membranous beta catenin in a cell occurred ; however , nuclear beta catenin and Tcf 4 proteins were markedly reduced by inhibitors and this lead to the diminished association of beta catenin with Tcf 4 and to the reduced binding to the consensus DNA . ^^^ In the present study , we demonstrate that curcumin and its derivative are excellent inhibitors of beta catenin / Tcf signaling in all tested cancer cell lines and the reduced beta catenin / Tcf transcriptional activity is due to the decreased nuclear beta catenin and Tcf 4 . . ^^^ |
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| Interacting proteins: P15884 and P35222 |
Pubmed |
SVM Score :0.0 |
| Surprisingly , these TCF 4 frameshift mutants lost transcriptional activity with beta catenin and down regulate the target gene expression . ^^^ |
|
| Interacting proteins: P15884 and P35222 |
Pubmed |
SVM Score :0.0 |
| As a result , our data reveal that the association of beta catenin and Tcf 4 is disrupted and the amount of beta catenin product in the nucleus is decreased by ionomycin in a concentration dependent manner . ^^^ |
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| Interacting proteins: P15884 and P35222 |
Pubmed |
SVM Score :0.0 |
| METHODS : The effects of K ras ( Val 12 ) on VEGF and T cell factor 4 ( TCF 4 ) promoter activity , nuclear levels of beta catenin and beta catenin / TCF 4 complexes , glycogen synthase kinase 3beta ( GSK 3beta ) phosphorylation , and GSK 3beta kinase activity were measured . ^^^ K ras ( Val 12 ) increased the stability of beta catenin , the levels of nuclear beta catenin , and the formation of nuclear beta catenin / TCF 4 complexes , and these effects were also blocked by LY 294002 . ^^^ |
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| Interacting proteins: P15884 and P35222 |
Pubmed |
SVM Score :0.0 |
| Aberrant transactivation of these TCF 4 regulated genes by beta catenin protein plays a crucial role in early intestinal carcinogenesis , and the transcriptional machinery of the TCF 4 / beta catenin complex is likely to contain targets for molecular therapy . ^^^ We explored the molecular composition of the TCF 4 / beta catenin transcriptional complex by means of proteomics . ^^^ PARP 1 physically interacted with TCF 4 and augmented the transcriptional activity of the beta catenin / TCF 4 complex . ^^^ CONCLUSIONS : In this study , we identified PARP 1 as a novel coactivator of the beta catenin / TCF 4 complex . ^^^ |
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| Interacting proteins: P15884 and P35222 |
Pubmed |
SVM Score :0.0 |
| Most sporadic colorectal cancers are initiated by activating Wnt pathway mutations , characterized by the stabilization of beta catenin and constitutive transcription by the beta catenin / T cell factor 4 ( Tcf 4 ) complex . ^^^ |
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| Interacting proteins: P15884 and P35222 |
Pubmed |
SVM Score :0.0 |
| Transactivation of MCP 1 / CCL2 by beta catenin / TCF 4 in human breast cancer cells . ^^^ In addition , the MCP 1 promoter was activated in BT 549 breast cancer cells transfected with beta catenin and TCF 4 cDNAs . ^^^ |
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| Interacting proteins: P15884 and P35222 |
Pubmed |
SVM Score :0.0 |
| Here we show that phosphorylated c Jun interacts with the HMG box transcription factor TCF 4 to form a ternary complex containing c Jun , TCF 4 and beta catenin . ^^^ Chromatin immunoprecipitation assays revealed JNK dependent c Jun TCF 4 interaction on the c jun promoter , and c Jun and TCF 4 cooperatively activated the c jun promoter in reporter assays in a beta catenin dependent manner . ^^^ Therefore , the phosphorylation dependent interaction between c Jun and TCF 4 regulates intestinal tumorigenesis by integrating JNK and APC / beta catenin , two distinct pathways activated by WNT signalling . . ^^^ |
|
| Interacting proteins: P15884 and P35222 |
Pubmed |
SVM Score :0.0 |
| As a result , our data revealed that the beta catenin distribution and the levels of nuclear beta catenin and Tcf 4 proteins were unchanged after naringenin treatment . ^^^ |
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| Interacting proteins: P15884 and P35222 |
Pubmed |
SVM Score :0.0 |
| Expression of a dominant negative Tcf 4 protein inhibited proliferation of RWP 1 cells but not in other lines lacking beta catenin dependent reporter activity , supporting the functional relevance of this mutation . ^^^ |
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| Interacting proteins: P15884 and P35222 |
Pubmed |
SVM Score :0.0 |
| TIS 7 regulation of the beta catenin / Tcf 4 target gene osteopontin ( OPN ) is histone deacetylase dependent . 12 O Tetradecanoylphorbol 13 acetate induced sequence 7 ( TIS 7 ) acts as a transcriptional co repressor interacting with SIN 3 , the histone deacetylase containing complex . ^^^ Osteopontin ( OPN ) , a known beta catenin / T cell factor 4 ( Tcf 4 ) downstream target gene , is up regulated in tumors and in cells with increased motility such as muscle cells . ^^^ TIS 7 has the capacity to down regulate beta catenin / Tcf 4 transcriptional activity . ^^^ We propose that TIS 7 down regulates the beta catenin / Tcf 4 transcriptional activity via its interaction with histone deacetylase containing complex thereby inhibiting the expression of beta catenin downstream target genes such as c Myc and OPN . ^^^ We hypothesize that TIS 7 as a negative regulator of transcriptional activity represses expression of OPN and beta catenin / Tcf 4 target genes , which are involved in myogenesis , muscle maintenance , and regeneration in a histone deacetylase dependent manner . . ^^^ |
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| Interacting proteins: P15884 and P35222 |
Pubmed |
SVM Score :0.0 |
| Immunoprecipitation studies demonstrated association of beta catenin with AR and T cell factor 4 ( TCF 4 ) in the presence of androgens . ^^^ |
|
| Interacting proteins: P15884 and P35222 |
Pubmed |
SVM Score :0.0 |
| Because nuclear complex consisting of TCF / LEF ( TCF 1 , TCF 3 , TCF 4 or LEF 1 ) , beta catenin , PYGO ( PYGO 1 or PYGO 2 ) and Legless ( BCL 9 or BCL9L ) binds to the TCF / LEF binding site to up regulate WNT / beta catenin target genes , FGF 16 gene was characterized as the evolutionarily conserved target of the WNT / beta catenin signaling pathway , just like FGF 18 and FGF 20 genes . ^^^ |
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| Interacting proteins: P15884 and P35222 |
Pubmed |
SVM Score :0.0 |
| Implications for the regulation of beta catenin / Tcf 4 dependent transcription . ^^^ Alzheimer disease linked Presenilin 1 ( PS 1 ) is a negative modulator of beta catenin / Tcf 4 activity . ^^^ |
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| Interacting proteins: P15884 and P35222 |
Pubmed |
SVM Score :0.0 |
| Nuclear translocations of beta catenin and TCF 4 in gastric cancers correlate with lymph node metastasis but probably not with CD 44 expression . ^^^ Interaction of nuclear beta catenin and TCF 4 is the end point of canonical Wnt signaling , which is believed to trigger the transcription of multiple cancer associated genes , including CD 44 . ^^^ So far , the combined status of beta catenin and TCF 4 and its relevance for lymph node metastasis and CD 44 expression have not been well studied in gastric cancers ( GCs ) . ^^^ Frequent TCF 4 up regulation and nuclear translocation of beta catenin were found in both primary and metastatic tumors . ^^^ The frequency of variant CD 44 expression increased in parallel with stepwise gastrocarcinogenesis and tumor spread , but the rates of detection did not match that of nuclear beta catenin and TCF 4 , especially in the premalignant and noncancerous samples . ^^^ |
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| Interacting proteins: P15884 and P35222 |
Pubmed |
SVM Score :0.0 |
| To characterize the specificity of Wnt / beta catenin driven thyrocyte proliferation , we transfected primary thyrocytes and FTC 133 cells with dominant negative TCF 4 to block Wnt dependent pathways or with dominant negative CREB to inhibit the TSH / cAMP cascade . ^^^ In cells transfected with dominant negative CREB lithium stimulated proliferation was unchanged whereas blocking Wnt / beta catenin by dominant negative TCF 4 reduced proliferation by approx . 50 % . ^^^ |
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| Interacting proteins: P15884 and P35222 |
Pubmed |
SVM Score :0.0 |
| Androgen receptor ( AR ) interacts with beta catenin and can suppress its coactivation of T cell factor 4 ( Tcf 4 ) in prostate cancer ( PCa ) cells . ^^^ Pin 1 expression in LNCaP cells enhanced beta catenin / Tcf4 transcriptional activity , as assessed using Tcf 4 regulated reporter genes , and increased expression of endogenous Tcf 4 and c myc . ^^^ |
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| Interacting proteins: P15884 and P35222 |
Pubmed |
SVM Score :0.0 |
| RAS induced activation of the ERK pathway was reduced by the dominant negative form of TCF 4 , indicating that the ERK pathway regulation by APC / beta catenin signaling is , at least , partly caused by effects on beta catenin / TCF4 mediated gene expression . ^^^ |
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| Interacting proteins: P15884 and P35222 |
Pubmed |
SVM Score :0.0 |
| Src dependent activation of beta catenin was prevented by SKI 606 , a novel Src family kinase inhibitor , which also abrogated beta catenin nuclear function by impairing its binding to the TCF 4 transcription factor and its trans activating ability in colorectal cancer cells . ^^^ |
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| Interacting proteins: P15884 and P35222 |
Pubmed |
SVM Score :0.0 |
| The regulation of beta catenin activity is thought to occur mainly on the level of protein degradation , but it has been suggested that beta catenin nuclear localization and hence its transcriptional activity may additionally be regulated via nuclear import by TCF 4 and BCL 9 and via nuclear export by APC and axin . ^^^ We show that TCF 4 and BCL9 / Pygopus recruit beta catenin to the nucleus , and APC , axin and axin 2 enrich beta catenin in the cytoplasm . ^^^ TCF 4 , APC , axin and axin 2 move more slowly than beta catenin in their respective compartment , and concomitantly decrease beta catenin mobility . ^^^ |
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| Interacting proteins: P15884 and P35222 |
Pubmed |
SVM Score :0.0 |
| A 17 , 700 compounds subset of the Pharmacia corporate collection was docked to this hot spot with the QXP program ; 22 of the best scoring compounds were put into a biophysical ( NMR and ITC ) screening funnel , where specific binding to beta catenin , competition with Tcf 4 and finally binding constants were determined . ^^^ |
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| Interacting proteins: P15884 and P35222 |
Pubmed |
SVM Score :0.0 |
| HIC 1 attenuates Wnt signaling by recruitment of TCF 4 and beta catenin to the nuclear bodies . ^^^ This appears to be due to the ability of HIC 1 to associate with TCF 4 and to recruit TCF 4 and beta catenin to the HIC 1 bodies . ^^^ |
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| Interacting proteins: P15884 and P35222 |
Pubmed |
SVM Score :0.0 |
| Furthermore , different B progenitor maturation stages showed differential expression of Wnt receptors and co receptors , beta catenin , plakoglobin , LEF 1 and TCF 4 mRNAs , suggesting canonical Wnt signaling as a regulator of early B lymphopoiesis . ^^^ |
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| Interacting proteins: P15884 and P35222 |
Pubmed |
SVM Score :0.0 |
| Here we show that Tcf 1 and Tcf 4 are expressed in osteoblasts during development and after birth ; stabilization of beta catenin , an essential component of canonical Wnt signaling , in differentiated osteoblasts results in high bone mass while its deletion from differentiated osteoblasts leads to osteopenia . ^^^ |
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| Interacting proteins: P15884 and P35222 |
Pubmed |
SVM Score :0.0 |
| Nuclear extract from HCT 116 , HepG 2 , or DLD 1 cells bound to NOS 2 TBE1 or TBE 2 oligonucleotides in electrophoretic mobility shift assays and the specific protein DNA complexes were supershifted with anti beta catenin or anti Tcf 4 antibody . ^^^ Overexpression of beta catenin and Tcf 4 significantly increased both basal and cytokine induced NOS 2 promoter activity ( P < 0 . 01 ) , and the induction was dependent on intact TBE sites . ^^^ Overexpression of beta catenin or Tcf 4 increased NOS 2 mRNA and protein expression in HCT 116 cells . ^^^ |
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