Pubmed abstracts for Protein-Protein Interaction search result :

Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.86849491
VEGF binds and activates two tyrosine kinase receptors called VEGFR 1 and VEGFR 2 and neuropilin 1 . 0.86849491^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.50039805
VEGF A binds and activates two receptors , VEGFR 1 ( Flt 1 ) and VEGFR 2 ( KDR / Flk 1 ) . 0.50039805^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.79766284
VEGF A , the prototype VEGF ligand , binds and activates two tyrosine kinase receptors : VEGFR 1 ( Flt 1 ) and VEGFR 2 ( KDR / Flk 1 ) . 0.79766284^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.82986897
In this study , we demonstrate that Flt 1 encodes for a 180 kDa glycoprotein , binds VEGF with high affinity , undergoes autophosphorylation but does not generate any mitogenic response in transfected NIH3T3 fibroblasts . 0.82986897^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :1.1158296
Flt 1 and Flk 1 have been shown to play key roles in vascular development ; these two receptors bind and are activated by vascular endothelial growth factor ( VEGF ) . 1.1158296^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.72514533
While VEGF binds to two receptor protein tyrosine kinases , VEGFR 1 ( Flt 1 ) and VEGFR 2 ( KDR ) , most biological functions of VEGF are mediated via VEGFR 2 , and the role of VEGFR 1 is currently unknown . 0.72514533^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.57716219
CONCLUSIONS : Exogenous VEGF interacts with VEGFR 1 and VEGFR 2 on the surface and regulates the proliferation of injured alveolar lining epithelial cells in an autocrine or paracrine fashion . . 0.57716219^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.54365532
VEGF binds to the tyrosine kinase receptors VEGFR 1 and VEGFR 2 , and loss of VEGF or its receptors results in abnormal angiogenesis and lethality during development . 0.54365532^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :1.0253867
VEGF binds and activates two tyrosine kinase receptors , VEGFR 1 ( Flt 1 ) and VEGFR 2 ( KDR / Flk 1 ) , and stimulates endothelial cell growth , survival , and vascular permeability . 1.0253867^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.73173705
The function of VEGFR 1 was evaluated by treating two representative cell lines with VEGF B , a selective ligand for VEGFR 1 , and / or a specific anti VEGFR 1 antibody and assessing the effects on signaling , migration , invasion , and proliferation . 0.73173705^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.5245277
Flt 1 is one of the specific receptors for VEGF , and soluble flt 1 ( sflt 1 ) binds to VEGF and competitively inhibits it from binding to the receptors . 0.5245277^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.62888186
VEGF interacts with three subtypes of VEGF receptors occurring on the cellular membrane known as VEGFR 1 ( Flt 1 ) , VEGFR 2 ( Flk 1 / KDR ) , and VEGFR 3 ( Flt 4 ) . 0.62888186^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Design of a variant of vascular endothelial growth factor A ( VEGF A ) antagonizing KDR / Flk 1 and Flt 1 . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
RESULTS : Human neovascular membranes consistently expressed VEGF A , B , and C ; PlGF ; and VEGFR 1 and 2 . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Moreover , two VPF receptors , kdr and flt 1 , are overexpressed by papillary dermal microvascular endothelial cells . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
So far , two VEGF / VPF receptors ( VEGF / VPF R ) have been identified ( KDR , and flt 1 ) . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Two receptors for VEGF , KDR and flt 1 , were also demonstrated in most of the tumors examined . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Furthermore , RECs were shown to express mRNA encoding the VEGF receptor flt 1 by northern blot analysis . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Flt was recently shown to be the receptor for the endothelial cell mitogen vascular endothelial growth factor ( VEGF ) . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
VEGF possesses two high affinity receptors , KDR and FLT 1 . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
The specific receptors of VEGF , flt 1 and KDR were also detected in gastric mucosa . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Two receptors , KDR and Flt 1 , have been identified for VEGF . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
VEGF D does not bind to VEGFR 1 . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Reverse transcriptase PCR of Caco 2 cell RNA showed mRNA for the VEGF receptor flt 1 . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Supernatant VEGF levels were measured using a flt 1 receptor capture ELISA . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
These cells express transcripts of the two VEGF receptors KDR and Flt 1 . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
The VEGF isoforms bind with two tyrosine kinase receptors , KDR / flk 1 and flt 1 . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
IHC analysis of the VEGF receptor flt 1 showed strong tumour cell staining in M006XLo glioma cells . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
VEGF acts via two tyrosine kinase family receptors : VEGFR 1 ( Flt 1 ) and VEGFR 2 ( KDR / Flk 1 ) . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
The different VEGF ' s recognize signalling tyrosine kinase receptors ( Flt 1 , Flk 1 and Flt 4 ) and accessory receptors . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
We have recently found evidence indicating the neuropilins form complexes with another VEGF receptor , VEGFR 1 . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
This can be explained by the upregulation of the VEGF receptors ( KDR & flt 1 ) by thrombin treatment . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Other gels were supplemented with VEGF ( 165 ) or FLT 1 soluble receptor fusion protein to bind VEGF . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
The soluble form of VEGF receptor , FLT 1 ( sFLT 1 ) , is a potent antagonist of VEGF . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
In microvessels , the VEGF receptor VEGFR 1 ( flt 1 ) could be visualised as well . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
VEGF initiates signaling by dimerizing the receptors VEGFR 1 and VEGFR 2 . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
SU 5416 is a competitive inhibitor of VEGF receptor subtypes VEGFR 1 and VEGFR 2 and stem cell factor receptor c kit . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
The biological effects of VEGF are mediated by two tyrosine kinase receptors , Flt 1 ( VEGFr 1 ) and KDR ( VEGFR 2 ) . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Here we demonstrate that VEGF and its receptors VEGFR 1 and VEGFR 2 are expressed in human surgical samples of HD . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
A neoangiogenesis and blood cell gene cluster included LCK , HCK , FGR , MMP 9 , CSFR 1 , VEGF , FLT 1 , and KDR . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
The soluble form of flt 1 VEGF receptor inhibits VEGF activity in a dominant negative manner . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Plasma VEGF and the soluble form of its receptor Flt 1 ( sFlt 1 ) were measured by ELISA . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
VEGF interacts with its cognate receptors , KDR / Flk 1 and Flt 1 , and exerts its angiogenic effect . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Plasma vascular endothelial growth factor , soluble VEGF receptor FLT 1 , and von Willebrand factor in glaucoma . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
PCPV ( VR 634 ) VEGF bound VEGF receptor 2 ( VEGFR 2 ) but did not bind VEGFR 1 or VEGFR 3 . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Soluble VEGF receptor Flt 1 : the elusive preeclampsia factor discovered . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
We report here that VEGF secreted by RPE cells upregulates PEDF expression via VEGFR 1 in an autocrine manner . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Both VEGF receptors ( flt 1 and flk 1 ) were identified on endothelial cells of epicardial arteries and veins . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
The VEGFR 1 ligand VEGF B did not promote either angiogenesis or lymphangiogenesis . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
VEGF mediates its actions through activation of two receptor tyrosine kinases , VEGFR 1 and VEGFR 2 . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
PTC express high levels of vascular endothelial growth factor ( VEGF ) and VEGF receptor ( Flt 1 ) . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
There was expression of VEGF R ( Flt 1 ) on the surfaces of both HL 60 and Raji cells . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
In addition , the VEGF receptors neuropilin 1 and flt 1 were also upregulated . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
However , bFGF induced dose dependent phosphorylation of Flt 1 and another VEGF receptor , KDR , in HUVECs but not SMCs . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
The mRNA for two VEGF receptors , Flt 1 and Flk 1 , was present in rMVECs and cMVECs . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
VEGF receptors VEGFR 2 ( KDR , flk 1 ) and VEGFR 1 ( flt 1 ) could be immunostained on osteoclasts and osteoblasts . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Systemic delivery of a VEGF antagonist , soluble Flt 1 , also inhibited the VEGF induced increase in CECs . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
We also performed immunohistochemical staining for the VEGF receptors Flt 1 and KDR . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
In contrast , VEGF A binds VEGFR 1 and VEGFR 2 and is an essential hemangiogenic factor . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
This indicates that , unlike in VEGF , glycosylation plays an important role in Flt 1 binding . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
The tyrosine kinases Flt 1 ( VEGFR 1 ) and Flk 1 / KDR ( VEGFR 2 ) are high affinity VEGF receptors . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
VEGF receptor Flt 1 and Flk 1 play key roles in angiogenesis . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
VEGF C and flt 1 were expressed by hepatic cells from weeks 7 to 12 of gestation . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
VEGF receptor 1 ( Flt 1 ) protein and mRNA were not changed by stretch . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Vascular endothelial growth factor ( VEGF ) and soluble VEGF receptor FLT 1 in diabetic nephropathy . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
This finding is consistent with the altered expression of vegf , flt 1 , kdr , and tie 1 in the mutant embryos . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Moreover , VEGF receptor Flt 1 levels increased , whereas KDR / Flk 1 decreased . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Expression of VEGF receptors Flt 1 and Flk 1 / KDR was heterogeneous across the cell lines . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
The gray scales of VEGF , Flt , KDT proteins in both A 549 cell lines and HUVEC 304 cell lines decreased ( P = 0 . 05 ) . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Soluble vascular endothelial growth factor , soluble VEGF receptor Flt 1 and endothelial function in healthy smokers . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
The tyrosine kinases Flt 1 ( VEGFR 1 ) and Flk 1 / KDR ( VEGFR 2 ) are high affinity VEGF receptors . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Somewhat unexpectedly , FLT 1 is the responsible VEGF receptor . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
We have shown that VEGF induces osteoclast differentiation through its action on VEGFR 1 . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Expression of VEGF and VEGF receptors , VEGFR 1 and VEGFR 2 , was also examined by RT PCR . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Moreover , VEGF receptors KDR and / or Flt 1 were decreased in BPD . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
This was associated with decreased VEGF levels as well as VEGFR 2 but not VEGFR 1 expression . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Prostate cancer cells expressed the mRNA for VEGF receptor ( VEGFR ) neuropilin 1 but not the VEGFRs Flt 1 or KDR . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
These effects were not observed in cells lacking Flt 1 , another VEGF receptor . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
RESULTS : VEGF , VEGFR 1 and VEGFR 2 positive cells were immunodetected in E 14 and E 18 bladders . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Bioactivity of TNF in tissues was confirmed by overexpression of Icam 1 , Sele , Vegfa , Flt 1 and Kdr . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Vascular endothelial growth factor A ( here referred to as VEGF ) is an endothelium specific growth factor that binds to two distinct receptor tyrosine kinases , designated Flt 1 and KDR / Flk 1 . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
We have used the natural antagonist of vascular endothelial growth factor A ( VEGF A ) , sflt 1 ( soluble form of flt 1 ) , to determine whether the edematous and proliferative effects of E 2 in the uterus are mediated by VEGF A . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
When we focus on the VEGF receptors , recent findings suggest a role of VEGFR 1 as a functional receptor for placenta growth factor ( PlGF ) and vascular endothelial growth factor A ( VEGF ) A in pericytes and vascular smooth muscle cells in vivo rather than in endothelial cells , and strongly suggest involvement of pericytes in early phases of angiogenesis . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
BACKGROUND AND OBJECTIVES : In tumors , vascular endothelial growth factor A ( VEGF A ) stimulates angiogenesis and vascular permeability by activating the tyrosine kinase receptor 2 ( VEGFR 2 or KDR / Flk 1 ) and 1 ( VEGFR 1 or Flt 1 ) . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Vascular endothelial growth factor A ( VEGF A ( 165 ) ) exerts multiple effects upon binding to the fms like tyrosine kinase 1 ( Flt 1 ) and the kinase insert domain containing receptor ( KDR ) . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
OBJECTIVE : To analyze the expression of vascular endothelial growth factor A ( VEGF A ) and receptors ( VEGFR 1 and VEGFR 2 ) in endometrial blood vessels , as well as microvascular density ( MVD ) , in endometrial biopsy samples from idiopathic menorrhagia patients . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Vascular endothelial growth factor A ( VEGF A ) and its 2 transmembrane tyrosine kinase receptors , VEGFR 1 and VEGFR 2 , constitute a ligand receptor signaling system that is crucial for developmental angiogenesis . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
The vascular endothelial growth factor A ( VEGF A ) is a cytokine that promotes angiogenesis through the activation of two tyrosine kinase receptors , VEGFR 1 and VEGFR 2 , on vascular endothelial cells . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Baseline vitreous and aqueous and serial postoperative aqueous samples were analysed for vascular endothelial growth factor A ( VEGF A ) , pigment epithelium derived Factor ( PEDF ) and other factors ( pg / ml ) including hepatocyte growth factor , MMP 9 , soluble flt 1 Receptor , and TGF beta 1 by ELISA . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Vascular endothelial growth factor A ( VEGF ) , which binds to both VEGF receptor 1 ( Flt 1 ) and VEGFR 2 ( KDR / Flk 1 ) , requires nitric oxide ( NO ) to induce angiogenesis in a cGMP dependent manner . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
To this end , expression of Vascular Endothelial Growth Factor A ( VEGF A ) , its receptors VEGFR 1 and 2 and microvessel density ( MVD ) were evaluated by quantitative immunohistochemistry in pretherapeutic biopsies of 40 patients with Ewing ' s sarcoma treated within standardised neoadjuvant protocols . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Expression of flt complementary DNA in COS cells conferred specific , high affinity binding of vascular endothelial growth factor , also known as vascular permeability factor ( VEGF VPF ) , a factor that induces vascular permeability when injected in the guinea pig skin and stimulates endothelial cell proliferation . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Vascular permeability factor receptor mRNA was strongly expressed in endothelial cells of small vessels adjacent to malignant tumor cells in DCIS , infiltrating ductal carcinoma , and metastatic ductal carcinoma . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Recently , the gene encoding vascular permeability factor ( VPF ) or vascular endothelial growth factor ( VEGF ) and the genes encoding its receptors ( kinase insert domain containing receptor ( KDR ) and fms like tyrosine kinase 1 [ fit 1 ] ) have been cloned . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Uterine smooth muscle cells express functional receptors ( flt 1 and KDR ) for vascular permeability factor / vascular endothelial growth factor . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Expression of vascular endothelial growth factor ( VEGF ) , also known as vascular permeability factor ( VPF ) , and its receptors Flt 1 and KDR ( Flk 1 in mouse ) and their localization in the human testis were analyzed by means of reverse transcriptase polymerase chain reaction ( RT PCR ) , Western blotting and immunohistochemistry . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
In an effort to obtain a more integrated understanding of vascular stroma formation in breast carcinoma , we examined expression of the angiogenic factor vascular permeability factor ( VPF ) / vascular endothelial growth factor ( VEGF ) ; the VPF / VEGF receptors flt 1 and KDR ; thrombospondin 1 , which has been reported to inhibit angiogenesis ; and the stromal components collagen type 1 , total fibronectin , ED A+ fibronectin , versican , and decorin by mRNA in situ hybridization on frozen sections of 113 blocks of breast tissue from 68 patients including 28 sections of breast tissue without malignancy , 18 with in situ carcinomas , 56 with invasive carcinomas , and 8 with metastatic carcinomas . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Dysregulation of Flt 1 , a major receptor for vascular permeability factor ( VPF ) , may provide a mechanism for the development of proteinuria in minimal change nephropathy ( MCN ) . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Vascular permeability factor ( VPF ) / vascular endothelial growth factor ( VEGF ) achieves its multiple functions by activating two receptor tyrosine kinases , Flt 1 ( VEGF receptor 1 ) and KDR ( VEGF receptor 2 ) , both of which are selectively expressed on primary vascular endothelium . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Endothelial cell stimulation by Tat occurs in the absence of activation of FLT 1 , another VEGF A tyrosine kinase receptor . . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
In addition , the soluble form of VEGFR 1 specifically inhibited polarization and migration induced by Tat and VEGF A , thus confirming the common use of this receptor . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Like VEGF A , VEGF E was found to bind with high affinity to VEGF receptor 2 ( KDR ) resulting in receptor autophosphorylation and a biphasic rise in free intracellular Ca2+ concentration , whilst in contrast to VEGF A , VEGF E did not bind to VEGF receptor 1 ( Flt 1 ) . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
This may reflect the different binding properties of VEGFs to VEGFRs , in that VEGF A binds to VEGFR 1 and 2 , whereas VEGF C acts through VEGFR 3 , whose expression becomes restricted to lymphatics and certain veins during development . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
While KDR expressing PAE / KDR cells responded to VEGF A stimulation with a significant elevation of intracellular cGMP already after 2 min , Flt 1 expressing PAE / Flt 1 cells did not show any signal in this RIA based cGMP assay . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
One of the key molecules promoting angiogenesis is the endothelial cell specific mitogen , vascular endothelial growth factor ( VEGF or VEGF A ) , which acts through two high affinity receptor tyrosine kinases ( VEGFR ) , VEGFR 1 ( or Flt 1 ) and VEGFR 2 ( or KDR / Flk 1 ) . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
VEGF A stimulates many actions of endothelial cells including proliferation , migration , and nitric oxide release via binding to and activation of the two primarily endothelial specific receptor tyrosine kinases KDR and Flt 1 . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Although treatment of KS 483 cells with soluble FLT 1 , an agent that blocks binding of VEGF A and B to VEGFR 1 , did not inhibit nodule formation , this observation does not exclude involvement of VEGFR 2 in the regulation of osteoblast differentiation . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
The chemotactic response of individual monocyte preparations to VEGF A , as mediated via Flt 1 , was quantitatively assessed using a modified Boyden chamber . ^^^ The VEGF A inducible kinase activity of Flt 1 , as assessed by in vitro kinase assays , remained intact in monocytes from diabetic patients . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
A possible contribution of VEGF A to the increased formation under hypoxic conditions is unlikely because there was no increased VEGF A expression detected under hypoxic conditions , and the hypoxia induced tube formation by FGF 2 and TNF alpha was not inhibited by soluble VEGFR 1 ( sVEGFR 1 ) , or by antibodies blocking VEGFR 2 . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Tyrosine phosphorylation of phospholipase Cgamma ( PLCgamma ) , tyrosine phosphatase SHP 2 , Crk , and extracellular regulated kinases 1 and 2 ( Erk1 / 2 ) was registered in response to VEGF A treatment of the PAE / VEGFR 1 cells . ^^^ PAE cells expressing the mutated VEGFR 1 Y1242F and Y1333F receptors mediated increased tyrosine phosphorylation of PLCgamma in response to VEGF A stimulation . ^^^ However , these two mutant VEGFR 1 failed to mediate increased mitogenesis and were unable to stimulate increased tyrosine phosphorylation of SHP 2 , Crk , and Erk1 / 2 , indicating that the mutations lead to a perturbation in VEGF A induced signal transduction . . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Correspondingly , the VEGF C specific receptor flt 4 and the VEGF A receptors flt 1 and flk 1 were up regulated in a temporal sequence similar to that of their agonist proteins in the cortical ring lesion and the region at risk . ^^^ This study suggests that VEGF C and its receptor flt 4 may cooperate with VEGF A and its receptors flt 1 and flk 1 to promote early angiogenesis after stroke , which may in turn contribute to spontaneous reperfusion in this focal thromboembolic stroke model . . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
These cells were found to express VEGF receptor 1 ( Flt 1 ) protein , and signaling by recombinant VEGF A ( 165 ) was demonstrated for two of the PEL cell lines , indicating the potential for autocrine , as well as paracrine , effects of viral cytokine induced VEGF . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Previously , we demonstrated that TNX binds to vascular endothelial growth factors A and B ( VEGF A and B ) and that VEGF B in combination with TNX induces DNA synthesis in endothelial cells via increased signals mediated by the VEGFR 1 receptor . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
VEGF A is a ligand for the two receptor tyrosine kinases VEGFR 1 ( Flt 1 ) and VEGFR 2 ( KDR / Flk 1 ) . ^^^ Most biological functions of VEGF A are mediated via VEGFR 2 , whereas the role of VEGFR 1 is largely unknown . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Involvement of VEGFR 2 ( kdr / flk 1 ) but not VEGFR 1 ( flt 1 ) in VEGF A and VEGF C induced tube formation by human microvascular endothelial cells in fibrin matrices in vitro . ^^^ Different forms of vascular endothelial growth factor ( VEGF ) and their cellular receptors ( VEGFR ) are associated with angiogenesis , as demonstrated by the lethality of VEGF A , VEGFR 1 or VEGFR 2 knockout mice . ^^^ Here we have used an in vitro angiogenesis model , consisting of human microvascular endothelial cells ( hMVEC ) cultured on three dimensional ( 3D ) fibrin matrices to investigate the roles of VEGFR 1 and VEGFR 2 in the process of VEGF A and VEGF C induced tube formation . ^^^ Soluble VEGFR 1 completely inhibited the tube formation induced by the combination of VEGF A and TNF alpha ( VEGF A / TNF alpha ) . ^^^ Blocking monoclonal antibodies specific for VEGFR 2 , but not antibodies specifically blocking VEGFR 1 , were able to inhibit the VEGF A / TNF alpha induced as well as the VEGF C / TNF alpha induced tube formation in vitro . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
The aim of this study was to examine immunohistochemically the presence of the VEGF family ( VEGF A to D ) and their receptors ( Flt 1 , Flk 1 , and Flt 4 ) in the surgically resected AVM nidus . ^^^ Formalin fixed , paraffin embedded specimens were stained immunohistochemically by the labeled streptavidin biotin method with antibodies against VEGF A to D , as well as Flt 1 , Flk 1 , and Flt 4 . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
METHODS : In retinas of 27 eyes of diabetic donors , 18 eyes of nondiabetic control donors , and 4 monkey eyes injected with PBS or VEGF A , expression patterns of VEGFR 1 , 2 , and 3 in relation to leaky microvessels , as identified by the marker pathologische anatomie Leiden endothelium ( PAL E ) were studied by immunohistochemistry . ^^^ In VEGF A injected monkey eyes , VEGFR 1 , 2 , and 3 and PAL E were expressed in retinal microvessels . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
An in vitro kinase assay failed to demonstrate activation of VEGFR 2 upon stimulation with either VEGF E or VEGF A , consistent with the idea that the effect of VEGF A on primary human osteoblasts is mediated via VEGFR 1 . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
VEGF A ( 165 ) , B , C , Flt 1 , and KDR were analyzed . ^^^ At protein level , VEGF B and C , Flt 1 , and KDR were not detectable in tissue lysates , whereas VEGF A was increased in stages 3 and 4 . ^^^ CONCLUSIONS : VEGF A ( 165 ) is one of the major angiogenesis regulators among the ligands ' family of VEGF , whereas its receptors KDR , and most probably Flt 1 , may contribute to a poor prognosis ( angiogenic ) phenotype , as indicated by their upregulated MRNA levels in stage 3 neuroblastoma . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Invasive cytotrophoblasts in early gestation expressed VEGF A , VEGF C , placental growth factor ( PlGF ) , VEGFR 1 , and VEGFR 3 and , at term , VEGF A , PlGF , and VEGFR 1 . ^^^ In severe preeclampsia and hemolysis , elevated liver enzymes , and low platelets syndrome , immunolocalization on tissue sections showed that cytotrophoblast VEGF A and VEGFR 1 staining decreased ; staining for PlGF was unaffected . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
To assess the contribution of NRP 1 to VEGF A mediated EC proliferation , migration , and PAF synthesis , we used porcine aortic EC ( PAEC ) recombinantly expressing Flt 1 , NRP 1 , KDR or KDR and NRP 1 . ^^^ Cells were stimulated with VEGF A , which binds to Flt 1 , KDR and NRP 1 , and VEGF C , which binds to KDR only . ^^^ In contrast , stimulation of PAEC Flt 1 and PAEC NRP 1 with VEGF A or VEGF C did not induce proliferation , migration , or PAF synthesis . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Co expression of VEGFR 1 , 2 , and 3 was observed in microvessels adjacent to epithelia in the eye , gastrointestinal mucosa , liver , kidney , and hair follicles , which is in line with the reported preferential expression of VEGF A in some of these epithelia . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Effect of hypoxia and endothelial loss on vascular smooth muscle cell responsiveness to VEGF A : role of flt 1 / VEGF receptor 1 . ^^^ METHODS AND RESULTS : Exposure to hypoxia induced a potentiation of cultured cell proliferation in response to either the agonist for the VEGF receptor 1 ( flt 1 ) placental growth factor ( PlGF 1 ) or to VEGF A . ^^^ CONCLUSION : These findings demonstrate that experimental conditions mimicking pathological vascular injury can make VSMCs responsive to VEGF A through the induction of functional flt 1 receptors . . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
The aim of this study was to localize members of the VEGF family ( VEGF A , B and C ) , and their receptors ( VEGFR 1 , 2 and 3 ) in human endometrial blood vessels . ^^^ Arterioles expressed VEGF B , VEGFR 1 , 2 , and 3 moderately and VEGF A variably . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
These data suggest a pivotal role for PlGF and VEGFR 1 in regulating VEGF A dependent angiogenesis under pathological conditions . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Structural modelling also revealed that a groove seen in the VEGF A homodimer and believed to play a role in its binding to VEGFR 1 is blocked in the viral VEGFs . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
VEGF A is a multifunctional cytokine that is widely expressed by tumor cells and that acts through receptors ( VEGFR 1 , VEGFR 2 , and neuropilin ) that are expressed on vascular endothelium and on some other cells . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Soluble VEGFR 1 , a receptor for VEGF A and B , inhibited tube formation by 87 % , a finding consistent with that of VEGF B inhibition . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
We measured the gene expression of VEGF ligands ( VEGF A , VEGF B , VEGF C , and VEGF D ) and their receptors ( VEGFR 1 , VEGFR 2 , and VEGFR 3 ) , in normal colorectal tissues ( n = 20 ) , adenomas ( n = 10 ) , and in CRC ( n = 71 ) representing different Duke ' s stages using ribonuclease protection assay , semi quantitative relative reverse transcriptase polymerase chain reaction , together with the pattern of their expression by immunohistochemistry . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
To investigate the mechanisms responsible for survival of blood vessels in the developing retina , we characterized two VEGF A receptors , VEGF receptor 1 ( VEGFR 1 , also known as Flt 1 ) and VEGF receptor 2 ( VEGFR 2 , also known as Flk 1 ) . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Although VEGF A binds two receptors , VEGFR 1 and 2 , a newly isolated ligand VEGF E ( Orf virus derived VEGF ) binds and activates only VEGFR 2 . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Neovascularization in the human heart is associated with expression of VEGF A and its receptors Flt 1 ( VEGFR 1 ) and KDR ( VEGFR 2 ) . ^^^ The transplanted muscle appeared severely degenerated and showed no immunoreactivity for von Willebrandt factor ( vWF ) and for VEGF A nor for its receptors KDR and Flt 1 . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
In compromised pregnancies , elevated levels of VEGF A and VEGFR 1 mRNAs may reflect the hypoxic status of the placenta . ^^^ Preeclampsia was associated with low levels of circulating PlGF and increased levels of total VEGF A and soluble VEGFR 1 . ^^^ The first one is related to an overproduction of competitive soluble VEGFR 1 that may lead to suppression of VEGF A and PlGF effects . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Fms like tyrosine kinase 1 ( Flt 1 ) / vascular endothelial growth factor ( VEGF ) receptor 1 , a receptor for VEGF A and placental growth factor , is expressed in the spongiotrophoblast layer that segregates the maternal and fetal vasculature in the mouse placenta . ^^^ Fms like tyrosine kinase 1 ( Flt 1 ) / vascular endothelial growth factor ( VEGF ) receptor 1 , a receptor for VEGF A and placental growth factor , is expressed in the spongiotrophoblast layer that segregates the maternal and fetal vasculature in the mouse placenta . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
After VEGF A treatment , the pre existing iris vasculature showed increased permeability , hypertrophy , and activation , as demonstrated by increased staining of CD 31 , PAL E , tPA , uPA , uPAR , Glut 1 , and alphavbeta 3 and alphavbeta 5 integrins , VEGF receptors VEGFR 1 , 2 and 3 , and Tie 2 in endothelial cells , and of NG 2 proteoglycan , uPA , uPAR , integrins and VEGFR 1 in pericytes . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Acute hypoxia selectively enhances at least three splice variants of VEGF A , and also selectively up regulates VEGFR 1 ( flt 1 ) . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Presence of GMP was associated with increased expression in tumour endothelium of the VEGF A receptors KDR , FLT 1 and neuropilin 1 , as well as VEGF D protein . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
We determined whether the expression of VEGF A and C and their receptors , Flt 1 and Flt 4 , are associated with primary tumor size , regional lymph node metastasis , distant metastasis and prognosis in patients with tongue carcinoma . ^^^ The expression of VEGF A and C , and their receptors , Flt 1 and Flt 4 , in biopsy specimens taken from 73 patients with tongue carcinoma were examined by immunohistochemical staining . ^^^ In terms of the overall 5 year survival rate , there was no significance correlation between the overall 5 year survival rate and expression of VEGF A , Flt 1 and Flt 4 expression , whereas there was a significant difference between VEGF C positive and VEGF C negative cases ( VEGF C positive , 51 . 7 % vs VEGF C negative , 94 . 2 % ) . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Regulation of VEGF A , VEGFR 1 , thrombospondin 1 , 2 , and 3 expression in a human pituitary cell line ( HP 75 ) by TGFbeta 1 , bFGF , and EGF . ^^^ We used the human pituitary cell line ( HP 75 ) to examine the effects of the growth factors TGFbeta 1 , bFGF , and EGF on cell growth , and on the regulation of the pro angiogenic growth factor VEGF A and the VEGFR 1 and the anti angiogenic molecules thrombospondin ( TSP ) TSP 1 and TSP 2 along with TSP 3 . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Angiopoietins ( Ang 1 and Ang 2 ) modulate the activity of the endothelial cell ( EC ) specific receptor tyrosine kinase Tie 2 , which together with vascular endothelial growth factor ( VEGF A ) and its EC specific receptors , VEGFR 1 and VEGFR 2 , regulate normal physiological vessel development . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
CONCLUSION ( S ) : The data confirm that CO 2 pneumoperitoneum is a cofactor in adhesion formation and demonstrate that VEGFR 1 plays a role in pneumoperitoneum enhanced adhesions , which is consistent with a role of placental growth factor , VEGF A , and VEGF B in pneumoperitoneum enhanced adhesions . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
In vivo angiogenesis assay using chick chorioallantoic membrane ( CAM ) showed that MCP 1 induced angiogenesis was as potent as that induced by VEGF A ( 165 ) and completely inhibited by a VEGF inhibitor , Flt ( 2 11 ) . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
We show here that the expression of VEGF A is dominant in the anterior portion of the embryo , whereas VEGFR 1 and VEGFR 2 are expressed in the posterior portion of the embryo . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
However , it is unknown whether VEGF A ( 165 ) is mediating PGI ( 2 ) synthesis through VEGF receptor 1 ( VEGFR 1 ) and / or VEGF receptor 2 ( VEGFR 2 ) activation and whether the coreceptor NRP 1 potentiates VEGF A ( 165 ) activity . ^^^ Treatment of BAEC with VEGF analogs , VEGF A ( 165 ) ( VEGFR 1 , VEGFR 2 and NRP 1 agonist ) and VEGF A ( 121 ) ( VEGFR 1 and VEGFR 2 agonist ) ( up to 10 ( 9 ) m ) , increased PGI ( 2 ) synthesis by 70 and 40 fold within 15 min . ^^^ Pretreatment with a VEGFR 2 inhibitor abrogated PGI ( 2 ) release mediated by VEGF A ( 165 ) and VEGF A ( 121 ) , and pretreatment of BAEC with antisense oligomers targeting VEGFR 1 or VEGFR 2 mRNA reduced PGI ( 2 ) synthesis mediated by VEGF A ( 165 ) and VEGF A ( 121 ) up to 79 % . ^^^ In summary , our data demonstrate that the activation of VEGFR 1 and VEGFR 2 heterodimer ( VEGFR 1 / R 2 ) is essential for PGI ( 2 ) synthesis mediated by VEGF A ( 165 ) and VEGF A ( 121 ) , which can not be reproduced by the parallel activation of VEGFR 1 and VEGFR 2 homodimers with corresponding agonists . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
We characterized , at the mRNA and protein levels , the expression of VEGF A and VEGF D and their cognate receptors , VEGFR 1 , VEGFR 2 , and VEGFR 3 in early and advanced stage prostate cancer specimens . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Treatment of the human CRC cell lines HT 29 and SW 480 with VEGF A ( a ligand for both VEGFR 1 and 2 ) or VEGF B ( a ligand specific for VEGFR 1 ) led to activation of Erk 1 / 2 , SAPK / JNK , and translocation of the p 65 subunit of nuclear factor kappaB into the nucleus . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
In this study , we first examined the expression of VEGF family ( VEGF A , B , C and D ) , VEGF A isoforms ( VEGF 120 , 164 , 188 ) , and VEGF specific receptors ( VEGFR 1 : Flt 1 ; VEGFR 2 : Flk 1 and VEGFR 3 : Flt 4 ) by quantitative RT PCR in lungs from young and old mice . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Inhibition of VEGFR 1 abrogated multiple myeloma cell proliferation and motility , suggesting that the functional interaction of VEGF A with its cognate receptor is essential for the growth of primary multiple myeloma cells . ^^^ Collectively , our results suggest that stromal dependent paracrine and intracrine VEGF A / VEGFR 1 signaling contributes to human primary multiple myeloma cell growth and therefore , VEGFR 1 blockade is a potential therapeutic strategy for the treatment of multiple myeloma . . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
We examined the expression of VEGF A and VEGFR 1 in human adult mesenchymal stem cells derived from trabecular bone ( hTBCs ) . ^^^ In addition , transcripts for VEGF A and the corresponding receptor VEGFR 1 were upregulated under hypoxic conditions in undifferentiated hTBCs . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
We used reverse transcriptase PCR to measure mRNA expression for VEGF A isoforms ( VEGF A ( 120 ) , ( 144 ) , ( 164 ) , and ( 188 ) ) and VEGF A receptors , Flt 1 and Flk 1 . ^^^ With advancing development , mRNA content increased only for VEGF A ( 188 ) ( p < 0 . 05 ) and for Flt 1 ( p < 0 . 02 ) and Flk 1 ( p < 0 . 005 ) . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Effects of recombinant VEGF A ( rcVEGF A ) , soluble flt 1 ( sFlt 1 ) receptor plus rcVEGF A , and control conditioned media were assessed in TCDD explants , while effects of TCDD conditioned media was assessed in control explants . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Formalin fixed , paraffin embedded sections of laryngeal squamous papillomas from the 12 patients with a diagnosis of RRP and the 5 control patients were examined by in situ hybridization for the presence of messenger RNA ( mRNA ) for VEGF A and vascular endothelial growth factor receptor 1 ( VEGFR 1 ) and vascular endothelial growth factor receptor 2 ( VEGFR 2 ) . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
These findings are consistent with the idea that the chemotactic effect of VEGF A on MPC is mediated via VEGFR 1 , and that VEGF A and PlGF 1 , have a functional role for recruitment of osteoprogenitor cells in the course of endochondral bone formation or remodeling . . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
These phenotypes depend strongly on p110gamma rather than on p85alpha and were associated with decreased expression of Ang 1 , VCAM 1 , connexin 40 , and ephrinB 2 but increased expression of Ang 2 , VEGF A , VEGFR 1 , and VEGFR 2 . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Current evidence suggests that the fenestrae of fully differentiated glomerular endothelium can be lost within hours if the VEGF A stimulus is removed , and that the glomerular endotheliosis , loss of glomerular filtration rate ( GFR ) and proteinuria observed in preeclampsia are due to the circulating inhibitor of VEGF A , soluble VEGF receptor 1 ( VEGFR 1 ) . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Unlike VEGF A , which binds to the two receptor tyrosine kinases VEGFR 1 ( Flt 1 ) and VEGFR 2 ( Flk 1 / KDR ) , VEGF B binds only to VEGFR 1 and the functional significance of VEGFR 1 signalling has remained problematic . ^^^ More recently , however , evidence has emerged suggesting a key role for VEGFR 1 signalling in pathological angiogenesis and this has raised the possibility that , like VEGF A , VEGFR 1 specific ligands such as VEGF B may provide for novel therapeutic strategies and / or represent new therapeutic targets . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Breast cancer cells express VEGF A , VEGF B , VEGF C and their receptors VEGFR 1 ( Flt 1 ) , VEGFR 2 ( Flk 1 / KDR ) and neuropilin ( NP 1 / NP 2 ) . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
However , the mechanism of PlGF and VEGFR 1 mediated angiogenesis has remained unclear and some in vitro studies suggest that VEGF A / VEGFR 2 signaling may also play a role in PlGF mediated angiogenesis . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Our treatment of the human pancreatic cancer cell line L3 . 6pl with the VEGFR 1 ligands VEGF A and VEGF B led to morphologic changes characteristic of EMT , including loss of polarity , increased intercellular separation , and the presence of pseudopodia . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Selective activation of Flt by placental growth factor demonstrated the importance of this receptor / kinase in the VEGF A responsiveness of pMC . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Loss of SPARC mediated VEGFR 1 suppression after injury reveals a novel antiangiogenic activity of VEGF A . ^^^ Excess VEGF A increased CNV before injury because VEGFR 1 activation was silenced by secreted protein , acidic and rich in cysteine ( SPARC ) . ^^^ The transient decline of SPARC after injury revealed a temporal window in which VEGF A signaling was routed principally through VEGFR 1 . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
In the present study , the expression of all VEGFs ( VEGF A , B , C , and D ) and their receptors ( VEGFR 1 , 2 , and 3 ) has been assessed in 39 primary human brain tumours . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Here , we examined the expression of VEGF A and its receptors VEGFR 1 , VEGFR 2 , NP 1 , and NP 2 in conditionally immortalized mouse podocytes cultured in undifferentiated and differentiated conditions using RT PCR and Western analysis . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
VEGF B is closely related to VEGF A and placenta growth factor ( PlGF ) , but unlike VEGF A , which binds to two receptor tyrosine kinases VEGFR 1 ( Flt 1 ) and VEGFR 2 ( Flk 1 / KDR ) , VEGF B and PlGF bind to VEGFR 1 and not VEGFR 2 . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
In addition to VEGF A , the cells expressed mRNAs for various members of the VEGF family and for their receptors , including VEGF B , C , D , flt 1 , and KDR . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Quail embryos were injected with soluble vascular endothelial growth factor ( VEGF ) receptors R 1 ( Flt 1 ) , R 2 ( Flk 1 ) , R 3 ( Flt 4 ) , VEGF Trap ( a chimera of R 1 and R 2 ) , or neutralizing antibodies to VEGF A , VEGF B , or fibroblast growth factor ( FGF ) 2 . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Cellular GSH levels , GSH : GSSG ratios , VEGF A mRNA and protein expression , as well as VEGF A secretion , and VEGFR 1 and VEGFR 2 receptor expression were determined . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
For example , levels of proangiogenic VEGF A , VEGF B , neuropilin 1 , VEGFR 1 , and VEGFR 2 were reduced and the levels of antiangiogenic thrombospondin 1 and retinoblastoma like 2 were increased . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
The perivascular CD 68 ( + ) cells expressed VEGFR 1 and VEGF A . ^^^ CONCLUSIONS : Heightened stromal hemangiogenesis as marked by infiltration of proangiogenic VEGFR 1 ( + ) CD 68 ( + ) VEGF A ( + ) cells and their paracrine cross talk with neovasculature appears to be a distinct feature of aggressive lymphoma , providing novel targets for antiangiogenic therapy , whereas alpha SMA ( + ) stromal vascular network may be differentially targeted in CLL / SLL . . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Secondly , we identified significantly increased levels of the potent VEGF A inhibitor , the soluble form of the VEGF receptor VEGFR 1 ( sVEGFR 1 ) in non healing wounds when compared to healing wounds . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
In addition , two VPF / VEGF receptors , flt 1 and kdr , are overexpressed by endothelial cells that line the microvessels that supply these tumors / inflammatory reactions . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Moreover , the VPF / VEGF receptors , flt 1 and KDR , were up regulated in endothelial cells in superficial dermal microvessels . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
In contact reactions , mRNAs for two VPF / VEGF vascular endothelial cell receptors , flt 1 and KDR , were also strikingly overexpressed . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
In situ hybridization studies that were performed on joint tissues from patients with active RA revealed that synovial lining macrophages strongly expressed VPF / VEGF mRNA , and that microvascular endothelial cells of nearby blood vessels strongly expressed mRNA for the VPF / VEGF receptors , flt 1 and KDR . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Endothelial cells did not express detectable VPF mRNA , but did express high levels of mRNA for the VPF receptors flt 1 and KDR indicating that the endothelial cell staining likely reflects binding of VPF secreted by adjacent tumor cells . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
The endothelial cells of nearby stromal blood vessels also stained for VPF by immunohistochemistry and in addition expressed mRNAs encoding the VPF receptors flt 1 and kdr as determined by in situ hybridization . ^^^ Endothelial cells away from the tumor did not stain for VPF and no definite mRNA expression for flt 1 or kdr was detected by in situ hybridization . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
VEGF / VPF expression by cells of hypoxic tissues coincides with expression of its two receptors , KDR and flt 1 , by ECs in the same tissues . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
VPF / VEGF stimulates endothelial cell growth and increases microvascular permeability by interacting with two endothelial cell tyrosine kinase receptors , KDR and flt 1 . ^^^ We studied 16 cases of AIDS associated Kaposi ' s sarcoma ( KS ) , 2 cases of cutaneous angiosarcoma , and 6 cases of capillary hemangioma by in situ hybridization for expression of VPF / VEGF , KDR , and flt 1 mRNAs . ^^^ The strong expression of both KDR and flt 1 in small stromal vessels in and around tumors suggests that VPF / VEGF may be an important regulator of the edema and angiogenesis seen in these tumors . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
The purpose of the present study was to define the expression of VPF / VEGF and its receptors flt 1 and KDR in ovarian tumors . ^^^ In addition , microvascular endothelial cells strongly expressed mRNA of the VPF / VEGF receptors flt 1 and KDR and immunostained for VPF / VEGF protein in the majority of malignant and borderline tumors examined . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Histologic sections were subjected to in situ hybridization using 35S labeled riboprobes specific for VPF / VEGF and , in a subset of cases , riboprobes specific for the VPF / VEGF receptors flt 1 and KDR . ^^^ In contrast , no strong expression of VPF / VEGF , flt 1 , or KDR mRNA was observed in the seven examples of benign atrophic endometrium studied . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
The expression of the VPF / VEGF receptor Flt 1 was selectively induced on dermal microvessels in skin explant cultures and in dermal endothelial cell monolayer cultures under hypoxic conditions . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
The high affinity tyrosine kinase receptor for VEGF , flt , although not expressed in normal brain endothelium , is upregulated in tumour endothelial cells in vivo . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
The KDR receptor tyrosine kinase shares structural similarities with a recently reported receptor for VEGF , flt , in a manner reminiscent of the similarities between the alpha and beta forms of the PDGF receptors . . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
VEGF neutralizing chimeric proteins were constructed by joining the extracellular domain of either human ( Flt ) or mouse ( Flk ) high affinity VEGF receptors with IgG . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
We examined the temporal and spatial expression of VEGF and its receptors , Flk 1 and Flt 1 , in the mouse uterus during the peri implantation period ( days 1 8 ) using Northern and in situ hybridization to assess the involvement of VEGF in the process of implantation . ^^^ The expression of the VEGF receptors , Flk 1 and Flt 1 , was also examined . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
The two genes for VEGF receptors , kinase insert domain containing receptor ( kdr ) and fms like tyrosine kinase 1 ( flt 1 ) , were found to be constitutively expressed in endothelial cells , and their relative mRNA levels were ranked in that order . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Recently , the tyrosine kinase receptors encoded by the flt and KDR / flk 1 genes were found to function as VEGF 165 receptors . . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
We have previously suggested that tumor angiogenesis in human gliomas is regulated by a paracrine mechanism involving vascular endothelial growth factor ( VEGF ) and flt 1 ( VEGF receptor 1 ) . ^^^ VEGF , an endothelial cell specific mitogen , is abundantly expressed in glioma cells which reside along necrotic areas , whereas flt 1 , a tyrosine kinase receptor for VEGF , is expressed in tumor endothelial cells , but not in endothelial cells in normal adult brain . ^^^ We performed in situ hybridization for VEGF mRNA , flt 1 mRNA and KDR mRNA on serial sections of normal brain , low grade and high grade glioma specimens . ^^^ Since flt 1 and KDR are not expressed in endothelial cells in the normal adult brain , the coordinate up regulation of 2 receptors for VEGF appears to be a critical event which controls tumor angiogenesis . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
We investigated the expression of vascular endothelial growth factor ( VEGF ) , an endothelial cell specific mitogen which is also known to induce vascular permeability in vivo , and its high affinity tyrosine kinase receptors flt 1 and KDR . ^^^ Endothelial cells did not express detectable amounts of VEGF mRNA but coexpressed flt 1 and KDR . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Expression and subtype analysis of vascular endothelial growth factor ( VEGF ) and its receptor ( flt 1 ) in human ovarian tumors ] . ^^^ Expression of flt 1 , a receptor for VEGF was detectable by PCR but not by Northern blot analysis . ^^^ These results suggest that like other epithelial cell derived carcinomas , ovarian tumors use the VEGF / flt 1 system for tumor angiogenesis . . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
However , expression of the genes encoding vascular endothelial growth factor ( VEGF ) and the high affinity tyrosine kinase receptor for VEGF ( flt 1 ) in human corneal endothelial cells could be demonstrated for the first time . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
The vascular endothelial growth factor ( VEGF ) and its high affinity binding receptors , the tyrosine kinases Flt 1 and Flk 1 , are thought to be important for the development of embryonic vasculature . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
We describe here that cultured human retinal pigment epithelial ( HRPE ) cells express both KDR and flt 1 receptors , bind VAS / VEGF on two high affinity sites ( apparent Kd of 9 and 210 pM corresponding to 940 and 18 , 800 sites per cell ) and proliferate or migrate upon recombinant VAS / VEGF addition . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
We also examined the expression of the other VEGF receptor , flt 1 , during placental development . flk 1 , tek , and tie transcripts were detected sequentially at one half day intervals starting at E7 . 0 , suggesting that each of these RTKs play a unique role during vascularization of the mouse embryo . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
To elucidate the pathogenesis of thyroid gland hypervascularity in patients with Graves ' disease , we studied the expression of mRNAs for vascular endothelial growth factor ( VEGF ) and its receptor , Flt family , using human thyroid follicles in vitro and thiouracil fed rats in vivo . ^^^ The thyroid follicles produced VEGF mRNA but not flt 1 mRNA . ^^^ VEGF mRNA was also increased on day 3 , accompanied by an increase in flt family ( flt 1 and KDR / flk 1 ) mRNA expression . ^^^ VEGF , a secretable angiogenesis factor , subsequently stimulates Flt receptors on endothelial cells in a paracrine manner , leading to their proliferation and producing hypervascularity of the thyroid gland , as seen in patients with Graves ' disease . . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Immunohistochemical analyses using antibodies against VEGF , bFGF , their receptors ( KDR , flt 1 , bek , and flg ) , factor 8 , and proliferating cell nuclear antigen were carried out on archival specimens of 52 human colon carcinomas and 10 adenomas . ^^^ Expression of VEGF and KDR was higher in metastatic than in nonmetastatic neoplasms and directly correlated with the extent of neovascularization and the degree of proliferation , whereas expression of bFGF , flt 1 , bek , and flg did not differ among tumor types . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
We have recently shown that the fms like receptor tyrosine kinase ( flt ) is a receptor for VEGF . ^^^ The expression of flt in the endothelium of both developing and mature blood vessels suggests that VEGF might regulate endothelial differentiation , blood vessel growth , and vascular repair . . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
We used two transplantable rat glioma cells lines , C 6 and GS 9L , to analyze VEGF regulation in vitro and expression of VEGF and its high affinity tyrosine kinase receptors , flt 1 and flk 1 , in vivo . ^^^ In situ hybridization showed that VEGF is expressed in vivo in rat glioma cells which reside along necrotic areas and therefore closely mimicks the expression pattern of VEGF observed in human glioblastoma . flt 1 and flk 1 are specifically expressed in endothelial cells in the tumor and at the border between tumor and normal brain but are absent from endothelial cells in the normal brain proper . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Increased gene expression for VEGF and the VEGF receptors KDR / Flk and Flt in lungs exposed to acute or to chronic hypoxia . ^^^ We found that ex vivo perfusion of isolated lungs under hypoxic conditions ( when compared with normoxia ) caused an increase in lung tissue mRNA of VEGF and of the VEGF receptors KDR / Flk and Flt . ^^^ Chronic hypobaric hypoxia also increased lung tissue mRNA levels of VEGF , KDR / Flk , and Flt and the amount of VEGF protein . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
The known human receptors for VEGF , flt and KDR , are both cell surface tyrosine kinases and are expressed predominantly on endothelial cells . ^^^ METHODS : VEGF , flt , and KDR expression was localized by in situ hybridization and immunohistochemistry in frozen sections of primary tumors from five patients with ovarian carcinoma and from metastases of ovarian carcinoma from three different patients . ^^^ Reverse transcription followed by polymerase chain reaction ( RT PCR ) and an enzyme linked immunosorbent assay were used to analyze VEGF , flt , and KDR expression in six epithelial cell lines derived from ovarian carcinoma ascites from five additional patients . ^^^ RESULTS : Messenger RNAs ( mRNAs ) encoding VEGF , flt , and KDR were detected in primary ascitic cells and in three of four ovarian carcinoma cell lines examined by RT PCR . ^^^ VEGF immunoreactivity was concentrated in clusters of tumor cells and patches of stromal matrix . flt immunoreactivity was confined to tumor blood vessels , but flt mRNA was not detected by in situ hybridization . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Protein tyrosine kinases expressed in glomeruli and cultured glomerular cells : Flt 1 and VEGF expression in renal mesangial cells . ^^^ In addition , Flt 1 , an endothelial cell specific receptor for vascular endothelial growth factor ( VEGF ) , is expressed in renal mesangial cells and its gene expression is up regulated upon the stimulation of platelet derived growth factor ( PDGF ) with the concomitant up regulation of VEGF . ^^^ These data suggest that possible involvement of VEGF / Flt 1 system in cytokine induced mesangial cell proliferations . . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
VEGF receptor subtypes KDR and FLT 1 show different sensitivities to heparin and placenta growth factor . ^^^ Vascular endothelial growth factor ( VEGF ) is an angiogenic growth factor which binds to two structurally related tyrosine kinase receptors denoted KDR and FLT 1 . ^^^ Heparin augmented VEGF binding to KDR expressing cells ( ABAE and WM 35 ) , but inhibited VEGF binding to FLT 1 expressing cells ( SK MEL 37 and WM 9 ) . ^^^ The concentration of heparin required for half maximal stimulation of VEGF binding to KDR expressing cells ( 500 ng / ml ) was 25 times greater than that required for half maximal inhibition of binding to FLT 1 expressing cells ( 20 ng / ml ) . ^^^ PIGF 1 had a negligible or no effect on 125I VEGF binding to KDR expressing cells ( ABAE , WM 35 ) , but did complete for binding to FLT 1 expressing cells ( SK MEL 37 and WM 9 ) . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
YU ZAZ 6 human melanoma cells expressed the mRNA encoding the VEGF receptor flt 1 , but not the mRNA encoding the VEGF receptor KDR / flk 1 . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
This study is the first demonstration of the presence of flt on non endothelial cells in vivo and suggests a role for VEGF in the growth and differentiation of cytotrophoblast at implantation . . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
The expression of VEGF , the closely related placental growth factor ( PIGF ) , the newly cloned endothelial high affinity VEGF receptors KDR and FLT 1 , and the endothelial orphan receptors FLT 4 and Tie were analyzed by in situ hybridization in normal human brain tissue and in the following CNS tumors : gliomas , grades 2 , 3 , 4 ; meningiomas , grades 1 and 2 ; and melanoma metastases to the cerebrum . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Proteins of M ( r ) consistent with the VEGF receptors Flt 1 and Flk 1 / KDR were also tyrosine phosphorylated in stimulated cells . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
The FLT 4 gene encodes a tyrosine kinase receptor related to the two identified receptors for vascular endothelial growth factor ( VEGF ) , FLT 1 and FLK1 / KDR . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
We examined the ability of PIGF to bind to soluble VEGF receptors , Flt 1 and Flk 1 / KDR , and characterized the binding of PIGF to endothelial cells . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Vascular endothelial growth factor ( VEGF ) is a homodimeric peptide growth factor which binds to two structurally related tyrosine kinase receptors denoted Flt 1 and KDR . ^^^ Binding analyses using 125I VEGF revealed Kd values of 16 pM for Flt 1 and 760 pM for KDR . ^^^ The KDR expressing cells showed striking changes in cell morphology , actin reorganization and membrane ruffling , chemotaxis and mitogenicity upon VEGF stimulation , whereas Flt 1 expressing cells lacked such responses . ^^^ KDR was found to undergo ligand induced autophosphorylation in intact cells , and both Flt 1 and KDR were phosphorylated in vitro in response to VEGF , however , KDR much more efficiently than Flt 1 . ^^^ Members of the Src family such as Fyn and Yes showed an increased level of phosphorylation upon VEGF stimulation of cells expressing Flt 1 but not in cells expressing KDR . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Recently it has been shown that the FLT 1 and KDR / FLK 1 proteins function as high affinity receptors for vascular endothelial growth factor ( VEGF ) . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Vascular endothelial growth factor ( VEGF ) is a potent angiogenic factor which binds to two structurally similar receptor tyrosine kinases , KDR and FLT 1 . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
We isolated and determined the primary structure of the entire coding region of rat flt 1 ( fms like tyrosine kinase ) , a receptor for Vascular Endothelial Growth Factor ( VEGF ) . ^^^ The transcripts of another VEGF receptor KDR / Flk 1 structurally related to Flt 1 was also expressed specifically in sinusoidal endothelial cells . ^^^ Therefore two reciprocal communication systems , VEGF Flt receptor family and HGF Met receptor , may exist in hepatic tissue . . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Also , TGF beta treatment of endothelial cells , which express the FLT 1 and KDR / FLK 1 receptors for VEGF , did not cause VEGF induction . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Differential expression of the two VEGF receptors flt and KDR in placenta and vascular endothelial cells . ^^^ Recently the flt encoded tyrosine kinase was characterized as a receptor for VEGF . ^^^ Placenta growth factor ( PIGF ) , a growth factor significantly related to VEGF , is coexpressed with flt in placenta and human vascular endothelial cells . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
The newly cloned FLT 4 receptor tyrosine kinase gene encodes a protein related to the VEGF receptors FLT 1 and KDR / FLK 1 . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
The human transmembrane c fms like tyrosine kinase Flt 1 has recently been identified as a VEGF receptor . ^^^ VEGF and its receptors Flk 1 / KDR and Flt 1 may play a role in vascular development and regulation of vascular permeability . . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Recently , tyrosine kinase encoded by the flt gene was identified as a receptor for VEGF . ^^^ In these cases , expression of VEGF and its receptor ( flt ) mRNA was examined by in situ hybridization : sense and antisense probes for VEGF and flt mRNA were used . ^^^ The flt mRNA was expressed in AS cells in all 10 cases that were positive for VEGF mRNA . ^^^ Sense probe for VEGF and flt mRNA gave no positive reactions . ^^^ CONCLUSIONS : These findings suggest a presence of paracrine or autocrine mechanism of proliferation in AS through VEGF and its receptor flt . . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
To evaluate the role of VEGF receptor 1 / flt 1 ( VEGFR1 / flt 1 ) in the development of the vascular system , we have characterized the murine homolog of the human flt 1 gene and have analyzed its expression pattern during mouse embryogenesis . ^^^ Receptor binding studies using transfected COS cells revealed that the murine flt 1 gene encodes a high affinity receptor for VEGF . ^^^ The apparent Kd for VEGF binding , as determined by Scatchard analysis , was 114 pM , demonstrating that VEGFR1 / flt 1 has a higher affinity to VEGF than VEGF receptor 2 / flk 1 ( VEGFR2 / flk 1 ) . ^^^ The differential expression of the two VEGF receptors persisted in the developing placenta , where VEGFR1 / flt 1 mRNA was detected in the spongiotrophoblast layer , whereas VEGFR2 / flk 1 transcripts were present in the labyrinthine layer which is the site of VEGF expression . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
To analyze the molecular mechanisms underlying glioma angiogenesis , we studied the expression of vascular endothelial growth factor ( VEGF ) and its tyrosine kinase receptors VEGFR 1 and VEGFR 2 during normal brain development and glioma induced angiogenesis . ^^^ Our results suggest a paracrine control of angiogenesis and endothelial cell proliferation that is tightly regulated and transient in the embryonic brain , switched off in the normal adult brain , and turned on in tumor cells ( VEGF ) and the host vasculature ( VEGFR 1 and 2 ) during tumor progression . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Vascular endothelial cell growth factor ( VEGF ) is a ligand for the tyrosine kinase receptor Flk 1 / KDR and Flt 1 and is considered to be an endothelial cell specific mitogen that plays an important role in angiogenesis . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
The endothelial cell specific vascular endothelial growth factor ( VEGF ) and its cellular receptors Flt 1 and Flk 1 have been implicated in the formation of the embryonic vasculature . ^^^ However , because Flt 1 also binds placental growth factor , a VEGF homologue , the precise role of VEGF was unknown . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Also , the expression of VEGF and its two receptors , Flt 1 and Flk 1 / KDR , is related to the formation of blood vessels in mouse and rat embryos . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Migration of human monocytes in response to vascular endothelial growth factor ( VEGF ) is mediated via the VEGF receptor flt 1 . ^^^ Using Northern blot analysis , we further could show that human monocytes express only the gene for the VEGF receptor type , flt 1 , but not for the second known VEGF receptor , KDR . ^^^ The results presented here suggest that monocyte chemotaxis in response to VEGF and most likely to PlGF 152 is mediated by flt 1 and thus show a possible function for the VEGF receptor flt 1 . . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Angiogenesis , the sprouting of new blood vessels from pre existing ones , and the permeability of blood vessels are regulated by vascular endothelial growth factor ( VEGF ) via its two known receptors Flt 1 ( VEGFR 1 ) and KDR / Flk 1 ( VEGFR 2 ) . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Two high affinity receptors for VEGF , flt 1 and flk 1 , are expressed by endothelial cells both in normal islets and in the stages of tumorigenesis ; these receptors are not up regulated during this process . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
In this study , we examined gene expression of vascular endothelial growth factor ( VEGF ) ; its receptor , flt 1 ; basic fibroblast growth factor ; and transforming growth factors ( TGFs ) alpha and beta in 18 paired cases of human breast carcinomas and the adjacent nonneoplastic tissues . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
In first trimester placenta , VEGF immunoreactive protein was detected in cytotrophoblast shell suggesting a role in the regulation of cytotrophoblast growth and differentiation as they also expressed VEGF receptor ( flt 1 ) protein . ^^^ VEGF and flt 1 immunoreactive proteins were expressed in Hofbauer cells within the villous mesenchyme , macrophages and in maternal decidual cells while weak VEGF immunoreactive protein was seen in syncytiotrophoblast surrounding the placental villi in first and second trimester placentae . ^^^ At term , there was relatively weak VEGF and flt 1 immunostaining in the syncytiotrophoblast while intense VEGF immunostaining was seen in the Hofbauer and maternal decidual cells . ^^^ Extravillous trophoblast showed immunostaining for flt 1 but no staining for VEGF . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
To date , 3 structurally related cell surface receptors for VEGF , Flt 1 , Flt 4 and KDR , have been identified and shown to be human type 3 receptor tyrosine kinases . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
VEGF mediates endothelial cell proliferation , angiogenesis , vascular growth , and vascular permeability via the endothelial cell receptors , kinase insert domain containing receptor ( KDR ) / fetal liver kinase 1 ( Flk 1 ) and FLT 1 . ^^^ Furthermore , this mutational analysis implicates KDR , but not FLT 1 , in VEGF induction of endothelial cell proliferation . . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Vascular endothelial growth factor ( VEGF ) , a potent angiogenic factor , uses two receptor tyrosine kinases , FLK / KDR and FLT , to mediate its activities . ^^^ On the other hand , the 165 and 175 kDa complexes did not seem to contain FLK / KDR or FLT but instead appeared to contain novel VEGF receptors with relatively low molecular masses of approximately 120 and 130 kDa . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
The class 3 receptor tyrosine kinases KDR and Flt 1 are high affinity receptors for VEGF , while Flt 4 is a receptor for the recently identified VEGF C . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
In contrast , soluble FLT 1 receptor differentiated VEGF isoforms ( 165 , 165 / 110 , 110 , and 121 ) with apparent affinities of 10 , 30 , 120 , and 200 pM , respectively . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
The authors studied the hypoxic gene regulation of two known VEGF receptors ( KDR and Flt ) and its mechanism in cultured bovine retinal endothelial cells ( BREC ) . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Two distinct receptors for vascular endothelial growth factor ( VEGF ) , the tyrosine kinase receptors Flt 1 and Flk 1 / KDR , have been described . ^^^ In this study we show that monocytes , in contrast to endothelium , express only the VEGF receptor Flt 1 , and that this receptor specifically binds also the VEGF homolog placenta growth factor ( PlGF ) . ^^^ In contrast , endothelial cells expressing both the Flt 1 and the Flk 1 / KDR receptors produce more tissue factor upon stimulation with VEGF than after stimulation with PlGF . ^^^ These findings strongly suggest Flt 1 as a functional receptor for VEGF and PlGF in monocytes and endothelial cells and identify this receptor as a mediator of monocyte recruitment and procoagulant activity . . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
The final protein products were almost completely in the dimeric conformation , bound to VEGF receptor FLT 1 with a Kd of 30 pM , stimulated proliferation of human umbilical vein endothelial cells half maximally at a concentration of 30 pM , and induced in vivo neovascularization and vascular permeability on the chicken chorioallantoic membrane . . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Localization of VEGF and expression of its receptors flt and KDR in human placenta throughout pregnancy . ^^^ VEGF immunoreactivity tended to co localize with the staining for flt . ^^^ These results indicate that VEGF may exert an important role within both the placental villi and the maternal decidua in relation to the growth , differentiation and migration of trophoblast and that this is mediated primarily through the spatial and temporal regulation of the flt receptor rather than the KDR receptor . . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
VEGF exerts its effect through two known high affinity tyrosine kinase receptors , named kinase insert domain containing receptor ( KDR ) and the fms like tyrosine kinase ( Flt ) . ^^^ Hypoxia increased Flt gene expression in BRPCs ( 2 . 7 fold , P < 0 . 01 ) . 125I labeled VEGF binding analysis on BRPCs demonstrated two apparent high affinity receptor subtypes ( Kd = 14 and 215 pmol / l ) , with 2 . 9 10 10 ( 4 ) and 1 . 4 10 10 ( 5 ) receptors / cell , respectively . 125I VEGF affinity cross linking demonstrated VEGF specific binding complexes at 150 , 172 , 187 , and 200 kDa under reducing conditions . ^^^ These results suggest that two classes of high affinity VEGF receptors are present on BRPCs , at least one of which is analogous to Flt and is capable of intracellular protein phosphorylation . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Involvement of the VEGF Flt receptor family in angiogenesis ] . ^^^ Recently , endothelial cell specific growth factor ( VEGF ) and its receptors ( Flt family ) has been characterized , and this ligand tyrosine kinase receptor is considered to be one of the most important systems involved in angiogenesis . ^^^ Thus , the stimulation of VEGF Flt towards angiogenesis is through a paracrine mechanism . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
In contrast , expression of the gene encoding flt 1 , a receptor for VEGF , was only observed in dedifferentiated and not in differentiated human corneal endothelial cells . ^^^ Despite this expression of flt 1 , the dedifferentiated cells showed no mitogenic response to VEGF . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
VEGF receptors flt and KDR ( kinase domain receptor ) were also detected , suggesting autocrine regulation . ^^^ During the menstrual cycle , expression of flt was constant but that of KDR was increased in the luteal phase , at which time the cells migrated in response to VEGF . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
In venous and arterial endothelial cells , TNF alpha potently reduced mRNA transcripts of the two VEGF receptors ( KDR / flk 1 and flt 1 ) in a dose and time dependent fashion . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
To investigate the molecular mechanisms underlying neovascularization in human germ cell tumors ( GCTs ) , we analysed the expression of two angiogenic growth factors , vascular endothelial growth factor ( VEGF ) and placenta growth factor ( P1GF ) , and of their receptors ( FLT 1 ) and Flk 1 / KDR ) in a panel of testicular tumors . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Northern analysis showed increased expression for both VEGF receptors ( flk 1 , flt 1 ) as well as the bFGF receptor 1 ( FGFR 1 ) in the collateral perfused region compared with that in the normally perfused region . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
The endothelial cell mitogen vascular endothelial growth factor ( VEGF ) stimulates angiogenesis , and together with its two receptor tyrosine kinases VEGFR 1 ( FLT 1 ) and VEGFR 2 ( KDR ) , is up regulated during the malignant progression of gliomas . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
The expressions of vascular endothelial growth factor ( VEGF ) and its receptors , flt 1 and KDR / flk 1 , were studied by Northern blotting in isolated rat liver cells and 70 % resected rat liver . ^^^ VEGF was expressed in hepatocytes immediately after isolation , and both flt 1 and KDR / flk 1 were expressed in non parenchymal cells including sinusoidal endothelial cells . ^^^ VEGF , flt 1 , and KDR / flk 1 were also expressed in normal rat liver . ^^^ In 70 % resected rat liver , VEGF expression increased with a peak at 72 hr after the operation , followed by expressions of flt 1 and KDR / flk 1 increasing between 72 and 168 hr . ^^^ These results suggest that VEGF expression increases in regenerating hepatocytes , which may contribute to proliferation of sinusoidal endothelial cells of rat liver following partial resection , probably through flt 1 and KDR / flk 1 receptors upregulated on sinusoidal endothelial cells . . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
A soluble form of the vascular endothelial growth factor ( VEGF ) receptor FLT 1 was identified in conditioned culture medium of human umbilical vein endothelial cells . ^^^ The endogenous soluble FLT 1 ( sFLT 1 ) receptor is chromatographically and immunologically similar to recombinant human sFLT 1 and binds [ 125I ] VEGF with a comparable high affinity . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Although VEGF binds Flt family Flt 1 and KDR / Flk 1 tyrosine kinases at high affinity for signal transduction , biological activities and the receptors of PIGF have not been extensively studied . ^^^ Both PIGFs competed for the binding of 125I labeled VEGF to Flt 1 receptor but not to KDR / Flk 1 expressed on NIH3T3 cells . ^^^ These results indicate that Flt 1 , but not KDR / Flk 1 , is a receptor for PIGF , suggesting that the weak biological activities of PIGF are due to its use of only part of the available VEGF signaling . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
VEGF and flt . ^^^ Our study attempts to clarify the chronological expression of VEGF and its receptor ( flt ) system in experimental cerebral infarction . ^^^ The chronological expression of the VEGF / flt system was analyzed semiquantitatively by immunohistochemical means in infarcts with different time courses from 3 hours to 3 weeks . ^^^ RESULTS : VEGF and flt were expressed exclusively in the ischemic brain . ^^^ Both VEGF ( bound ) and flt were detected in endothelial cells along with the development of angiogenesis . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
The presence of mRNA for aFGF , bFGF , and three isoforms of VEGF , as well as their receptors , FGFR 1 , FGFR 2 , Flt 1 , and KDR , in vessel outgrowths and the parent vessel , as identified by RT PCR , strongly implicated aFGF , bFGF , and VEGF as having an important role in this neovascularization response . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Flt 1 is a high affinity binding receptor for the vascular endothelial cell growth factor ( VEGF ) and is primarily expressed in endothelial cells . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
The second immunoglobulin like domain of the VEGF tyrosine kinase receptor Flt 1 determines ligand binding and may initiate a signal transduction cascade . ^^^ Vascular endothelial growth factor ( VEGF ) is an angiogenic inducer that mediates its effects through two high affinity receptor tyrosine kinases , Flt 1 and KDR . ^^^ Here we show that deletion of the second domain of Flt 1 completely abolishes the binding of VEGF . ^^^ Introduction of the second domain of KDR into an Flt 1 mutant lacking the homologous domain restored VEGF binding . ^^^ We then created chimeric receptors where the first three or just the second Ig like domains of Flt 1 replaced the corresponding domains in Flt 4 , a receptor that does not bind VEGF , and analyzed their ability to bind VEGF . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Placenta growth factor ( PlGF ) is a growth factor which belongs to the vascular endothelial growth factor ( VEGF ) family and is known to bind to the fms like tyrosine kinase receptor ( flt 1 ) . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
VEGF , flk 1 , and flt 1 expression in a rat myocardial infarction model of angiogenesis . ^^^ Vascular endothelial growth factor ( VEGF ) is an endothelial cell mitogen that is thought to function by interacting with two high affinity receptors , flk 1 and flt 1 . ^^^ To determine the role played by VEGF , flk 1 , and flt 1 in this process in vivo , we studied the expression of the growth factor and its receptors in a rat infarct model . ^^^ After an acute myocardial infarction , we observed an initial rapid ( 1h ) rise in VEGF ( 275 % ) , flk 1 ( 375 % ) , and flt 1 ( 400 % ) mRNA expression throughout the entire heart . ^^^ Initial diffuse induction of VEGF , flk 1 , and flt 1 expression in the left ventricle was later replaced by an increase predominantly limited to perimyocardial infarction area where angiogenesis was taking place . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
However , the finding of dramatic up regulation of vascular endothelial growth factor ( VEGF ) , a potent endothelial cell growth factor with vascular permeability inducing activity , in stromal cells and the corresponding receptors , VEGFR 1 and VEGFR 2 , in tumor endothelial cells suggests that angiogenesis and cyst formation in hemangioblastomas may be regulated by this signaling pathway via a paracrine mechanism . . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Possible involvement of VEGF FLT tyrosine kinase receptor system in normal and tumor angiogenesis . ^^^ VEGF , which has recently been reported as a ligand for Flt 1 receptor , dramatically stimulated growth of endothelial cells in culture . ^^^ Many human tumors were found to express VEGF mRNA but not Flt 1 message , suggesting a paracrine mechanism for tumor angiogenesis . ^^^ Interestingly , VEGF could not stimulate proliferation of Flt 1 overexpressing NIH3T3 fibroblast cells . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
AIDS KS cells and primary tumor tissues also expressed high levels of Flt 1 and KDR , the receptors for VEGF , while the normal skin of the same patients did not show any expression . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Consequently , the in vivo regulation of VEGF , which might be modulated by induction of flt 1 receptor gene expression , differs from the in vitro cell culture situation and might be different from the EPO regulation in vivo . . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Tumor specimens from 109 patients with previously untreated squamous cell lung carcinomas were analyzed immunohistologically for the expression of vascular endothelial growth factor ( VEGF ) and its receptor Flt 1 . ^^^ VEGF staining was seen in 59 % and Flt 1 staining in 68 % of the cases . ^^^ No significant correlations were detected between VEGF or Flt 1 expression and stage or metastasis . ^^^ Combining VEGF and Flt 1 expressions did not improve the prognostic value . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
In this study , we have examined the expression of VEGF and its tyrosine kinase receptors ( flt and flk 1 ) in isolated rat islets of Langerhans in vitro . ^^^ Reverse transcriptase polymerase chain reaction revealed the presence of both flt and flk 1 in freshly isolated islets , and two VEGF isoforms , namely VEGF 120 and VEGF 164 . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Tumor specimens of non small cell lung carcinomas ( NSCLC ) from previously untreated patients ( n = 153 ) were analysed immunohistochemically for expression of vascular endothelial growth factor ( VEGF ) , VEGF receptor ( Flt 1 ) , basic fibroblast growth factor ( bFGF ) and FGF receptor ( FGFR 1 , ( Flg ) . ^^^ The data clearly demonstrate that a significant relationship exists between VEGF ( p < 0 . 001 ) and Flt 1 expression ( p < 0 . 01 ) and drug response . ^^^ The expression of VEGF and Flt 1 was lower in resistant than in sensitive tumors . ^^^ The data from this investigation clearly demonstrate that a significant interrelationship exists between the expression of VEGF , VEGF receptor Flt 1 and FGF receptor , FGFR 1 and the response of NSCLC to doxorubicin in vitro . . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Vascular Endothelial Growth Factor ( VEGF ) mediates its actions through the Flt 1 and KDR ( Flk 1 ) receptor tyrosine kinases . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
We obtained the following evidence that it is vascular endothelial growth factor ( VEGF ) that mainly mediates the angiogenic activities of AGE . ( 1 ) Quantitative reverse transcription polymerase chain reaction analysis of poly ( A ) + RNA from microvascular endothelial cells revealed that AGE BSA up regulated the levels of mRNAs for the secretory forms of VEGF in time and dose dependent manners , while endothelial cell expression of the genes encoding the two VEGF receptors , kinase insert domain containing receptor and fms like tyrosine kinase 1 , remained unchanged by the AGE treatment . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
The vascular endothelial growth factor ( VEGF ) receptor FLT 1 has been shown to be involved in vasculogenesis and angiogenesis . ^^^ Upon VEGF binding FLT 1 becomes phosphorylated , which has been thought to be preceded by receptor dimerization . ^^^ To further investigate high affinity binding of VEGF to FLT 1 and ligand induced receptor dimerization , we expressed in Sf 9 cells the entire extracellular domain comprising all seven Ig like loops : sFLT 1 ( 7 ) and several truncated mutants consisting of loop one , one and two , one to three , one to four , and one to five . ^^^ Dimerization of the extracellular domains of FLT 1 upon addition of VEGF was detected with all mutants containing the Ig like loop four . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
These findings indicate that VEGF expressed by RGCs under hypoxia or diabetic condition plays an integral role in the maintenance of retinal circulation via the VEGF VEGF receptor ( flt 1 ) interaction . . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Rabbit uteri were examined using Northern and in situ hybridization to assess the temporal and spatial expression of VEGF and its receptor ( Flk 1 , Flt 1 ) mRNAs during the pre and peri implantation periods ( Days 0 8 ) . ^^^ VEGF receptor mRNAs were expressed in the uterus at all stages examined , with high levels of Flk 1 and Flt 1 at estrus and again just before implantation , 6 3 / 4 days pregnant . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Vascular endothelial growth factor ( VEGF ) stimulated the tyrosine phosphorylation of multiple components in confluent human umbilical vein endothelial cells ( HUVECs ) including bands of Mr 205 , 000 , corresponding to the VEGF receptors Flt 1 and KDR , and Mr 145 , 000 , 120 , 000 , 97 , 000 , and 65 , 000 70 , 000 . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Role of VEGF receptor 1 ( Flt 1 ) in mediating calcium dependent nitric oxide release and limiting DNA synthesis in human trophoblast cells . ^^^ Vascular endothelial growth factor ( VEGF ) receptor KDR ( kinase insert domain containing receptor ) is linked to endothelial cell proliferation , and VEGF receptor Flt 1 ( fms like tyrosine kinase ) is essential for the organization of embryonic vasculature . ^^^ Herein we report that human trophoblast and endothelial cells contain functional Flt 1 receptors for VEGF that trigger the synthesis and release of nitric oxide ( NO ) by the activation of constitutive NO synthase ( cNOS ) . ^^^ VEGF neutralizing anti VEGF monoclonal antibody significantly inhibited VEGF mediated NO release ( p < 0 . 001 ) , and the addition of a neutralizing anti Flt 1 antibody inhibited the response by 79 . 6 % + / 7 . 59 % , an effect found to be reversible with higher concentrations of VEGF . ^^^ VEGF 165 stimulated NO release via phosphorylation of the Flt 1 receptor , indicating that VEGF may be an autocrine regulator of NO biosynthesis by aiding trophoblast penetration into spinal arterioles during the first trimester and preventing platelet aggregation within the placenta . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Vascular endothelial growth factor ( VEGF ) and its receptors , Flt 1 ( VEGFR 1 ) and Flk 1 ( VEGFR 2 ) , as well as Angiopoietin 1 and its receptor , Tie 2 , represent key signal transduction systems involved in the regulation of embryonic vascular development . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
A quantitative reverse transcription PCR study revealed that genes for VEGF receptors ( Flt 1 and KDR ) were expressed in HUVEC , but not in HOB , and that 1 , 25 ( OH ) 2D3 increased the levels of expression of VEGF receptor genes in endothelial cells only when cocultured with HOB . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
VEGF receptor tyrosine kinases , VEGF R 1 ( flt 1 ) and VEGF R 2 ( flk 1 ) , are induced in a tumour stage dependent manner during glioma progression and are exclusively expressed in tumour vascular endothelial cells . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
The vascular endothelial growth factor ( VEGF ) and its receptor ( Flt ) protein expression was immunologically studied individually and in combination with the microvascular density ( MVD ) determined by the factor 8 related antigen ( F8RA ) and their prognostic value in 118 patients with a pulmonary adenocarcinoma . ^^^ The presence of VEGF and Flt was associated with the MVD and prognosis . ^^^ A reduced expression of both of VEGF and Flt correlated with less tumor angiogenesis and a better prognosis . ^^^ Our findings suggest that VEGF and Flt expressions play an important role in tumor angiogenesis and that the VEGF and Flt coexpression promotes angiogenesis and metastasis in pulmonary adenocarcinoma . . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
MAIN OUTCOME MEASURE : mRNA levels of vascular endothelial growth factor ( VEGF ) and its receptor the fins like tyrosine kinase ( flt ) , were quantified in total RNA isolated from placental biopsies using the RNAse protection assay . ^^^ Results were expressed as arbitrary optical density units of VEGF / GAPDH and flt / GAPDH . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
In order to explore factors regulating such angiogenesis and cyst formation in pilocytic astrocytoma , we examined expression of VEGF and its receptors ( KDR and Flt 1 ) using in situ hybridization . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
FLT 4 represents a recently cloned member of class 3 receptor tyrosine kinases which include receptors for the angiogenic growth factor VEGF , namely FLT 1 and KDR . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Vascular endothelial growth factor ( VEGF ) is an angiogenesis factor for which two signaling protein tyrosine kinase receptors , Flt 1 and KDR , have been identified . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Vascular endothelial growth factor ( VEGF ) and its tyrosine kinase receptors VEGFR 1 ( flt 1 ) and VEGFR 2 ( flk 1 / KDR ) are key mediators of physiological and pathological angiogenesis . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Vascular endothelial growth factor ( VEGF ) and its two endothelial cell specific receptor tyrosine kinases , Flk 1 / KDR and Flt 1 , play a key role in physiological and pathological angiogenesis . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Flt 1 , a tyrosine kinase receptor for vascular endothelial growth factor ( VEGF ) , plays important roles in the angiogenesis required for embryogenesis and in monocyte / macrophage migration . ^^^ In Flt 1 overexpressing NIH3T3 cells , VEGF induced autophosphorylation of Flt 1 , tyrosine phosphorylation of PLCgamma and protein kinase C dependent activation of MAP kinase . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
VEGF binds to two different tyrosine kinase receptors , kinase domain receptor ( KDR ) and Fms like tyrosine kinase 1 ( Flt 1 ) , and a number of VEGF homologs are known with distinct patterns of specificity for these same receptors . ^^^ Mapping the receptor binding determinants on a multiple sequence alignment of VEGF homologs , suggests the differences in specificity towards KDR and Flt 1 may derive from both sequence variation and changes in the flexibility of binding loops . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Characterization of the VEGF / PIGF receptors , KDR and flt 1 , revealed the presence of flt 1 mRNA in isolated cytotrophoblast and in vitro differentiated syncytiotrophoblast . ^^^ In addition , presence of function flt 1 on normal trophoblast suggests that VEGF / PIGF functions in an autocrine manner to perform an as yet undefined role in trophoblast invasion , differentiation , and / or metabolic activity during placentation . . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Flt 1 tyrosine kinase , vascular endothelial growth factor ( VEGF ) receptor 1 , binds VEGF and a new VEGF related ligand , placenta growth factor , but KDR / Flk 1 ( VEGF receptor 2 ) binds only VEGF . ^^^ Soluble Flt 1 carrying the N terminal 1st to 3rd Ig region suppressed VEGF dependent endothelial proliferation in vitro to the same level as the larger forms of soluble Flt 1 , suggesting that the binding of one soluble Flt 1 molecule to one subunit of the VEGF homodimer may be sufficient to block the VEGF activity . . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Two VEGF receptors , fms like tyrosine kinase 1 ( FLT 1 ) and KDR , have been identified almost specifically on human endothelial cells . ^^^ Both mutant VEGF , which activates only KDR , and placenta growth factor , which activates only FLT 1 , were able to enhance FLT 1 expression . ^^^ VEGF stimulated FLT 1 mRNA expression was inhibited by actinomycin D . ^^^ These data suggest that VEGF itself is the main factor secreted by tumor cells that is able to enhance the expression of its receptor FLT 1 and of a soluble variant of FLT 1 in endothelial cells . . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Crystal structure at 1 . 7 A resolution of VEGF in complex with domain 2 of the Flt 1 receptor . ^^^ Vascular endothelial growth factor ( VEGF ) is a homodimeric hormone that induces proliferation of endothelial cells through binding to the kinase domain receptor and the Fms like tyrosine kinase receptor ( Flt 1 ) , the extracellular portions of which consist of seven immunoglobulin domains . ^^^ We show that the second and third domains of Flt 1 are necessary and sufficient for binding VEGF with near native affinity , and that domain 2 alone binds only 60 fold less tightly than wild type . ^^^ The crystal structure of the complex between VEGF and the second domain of Flt 1 shows domain 2 in a predominantly hydrophobic interaction with the `` poles ' ' of the VEGF dimer . ^^^ Based on this structure and on mutational data , we present a model of VEGF bound to the first four domains of Flt 1 . . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
It was previously shown that VEGF 165 and VEGF 121 both bind to KDR / Flk 1 and Flt 1 but that VEGF 165 binds in addition to a novel receptor ( Soker , S . , Fidder , H . , Neufeld , G . , and Klagsbrun , M . ( 1996 ) J . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
In the present study , expression of VEGF and its receptors , fetal liver kinase 1 ( flk 1 ) and fms like tyrosine kinase 1 ( flt 1 ) , was examined in rat carotid arteries after balloon injury . ^^^ Although VEGF and flk 1 were not detectable , high levels of flt 1 mRNA and protein were expressed by smooth muscle cells ( SMCs ) in the neointima , as demonstrated by en face in situ hybridization and Western blotting . ^^^ These findings demonstrate that vascular SMCs express functional flt 1 receptors after arterial injury and that VEGF has synergistic effects with FGF 2 on SMC proliferation . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Upregulation of the angiogenic factors PlGF , VEGF and their receptors ( Flt 1 , Flk 1 / KDR ) by TSH in cultured thyrocytes and in the thyroid gland of thiouracil fed rats suggest a TSH dependent paracrine mechanism for goiter hypervascularization . ^^^ Since goiters of the thyroid gland are extremely hypervascular , we investigated the expression of PlGF , VEGF and their receptors , Flt 1 and Flk 1 / KDR , in a small panel of human goiters from patients with Graves ' s disease , in an animal model of thyroid goitrogenesis and in in vitro cultured thyroid cells . ^^^ In vivo studies demonstrated that in the thyroid gland of thiouracil fed rats , increased mRNA and protein expression of PIGF , VEGF , Flt 1 and Flk 1 / KDR occurred subsequent to the rise in the serum thyroid stimulating hormone ( TSH ) levels and in parallel with thyroid capillary proliferation . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
In addition , the expression of vascular endothelial growth factor ( VEGF ) and its receptors , KDR , Flt 1 and Flt 4 in oral SCCs was examined in relation to the vessel density and lymph node metastasis . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
The following study investigated the expression of VEGF and its receptor flt 1 mRNA and protein in the pituitary gland of sheep . ^^^ Immunocytochemistry with a flt 1 antibody confirmed the expression of this high affinity receptor for VEGF in endothelial cells across the pituitary gland . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Recently , two endothelial cell surface receptors , flk 1 and flt 1 , have been shown to mediate the angiogenic activities of VEGF . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Flt 1 is one of two receptor tyrosine kinases through which the angiogenic factor vascular endothelial growth factor ( VEGF ) functions . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Vascular endothelial growth factor ( VEGF ) is a multifunctional cytokine , which has recently been reported to enhance the activation and migration of monocytes through the flt receptor in vitro , which are key events in granuloma formation of granulomatous disorders and in sarcoidosis . ^^^ Since activated macrophages and monocytes are known to be involved in sarcoid granuloma formation in sarcoidosis , we investigated the expression of VEGF and its receptor flt in 33 paraffin embedded lung tissue biopsies of patients with pulmonary sarcoidosis . ^^^ VEGF mRNA was localized by nonradioactive in situ hybridization , VEGF and flt expression were visualized immunohistochemically . ^^^ We found an increased transcription and protein production of VEGF and an overexpression of flt in activated alveolar macrophages , in epitheloid cells , and in multinuclear giant cells of pulmonary sarcoid granulomas . . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
VEGF binds to the related receptor tyrosine kinases Flt 1 and KDR / Flk 1 with high affinity , whereas PlGF binds only to Flt 1 . ^^^ We examined VEGF and PlGF for their capacity to stimulate signal transduction in porcine aortic endothelial cells expressing Flt 1 or KDR . ^^^ VEGF had essentially no effect on Flt 1 expressing cells , but induced DNA synthesis and migration of KDR expressing cells . ^^^ In agreement , MAP kinase , examined as a marker for DNA synthesis , was activated both by VEGF stimulation of the KDR cells and by PlGF stimulation of the Flt 1 cells . ^^^ In contrast , phospholipase C gamma ( PLC gamma ) , was tyrosine phosphorylated only in VEGF stimulated KDR cells , and not in the PlGF stimulated Flt 1 cells , which is in agreement with a role for PLC gamma in cellular migration . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
We studied the expression and function of VEGF and its receptors fms like tyrosine kinase ( Flt 1 ) and fetal liver kinase ( designated as kinase insert domain containing receptor , KDR in the human ) in the human epididymis . ^^^ VEGF may act as a paracrine effector to influence the permeability of lymphatic vessels via Flt 1 , and of blood vessels via KDR . . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
The expression of Flk 1 , and flt 1 ( the two VEGF receptors ) and also of tie 2 , which is crucial for blood island formation , was detected as early as day 4 , and also on days 8 and 21 . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
METHODS : Immunohistochemical staining of ERMs was done for PDGF A , PDGF B , basic fibroblast growth factor ( bFGF ) , three isoforms of transforming growth factor beta ( TGF beta ) , and vascular endothelial growth factor ( VEGF ) and its receptors , flt 1 and flk 1 / KDR . ^^^ The receptors for VEGF , flt 1 , and flk 1 / KDR were also identified on cells in ERMs and on cultured RPE cells . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Here we report the unexpected finding that treatment with truncated soluble Flt 1 receptors , which inhibit vascular endothelial growth factor ( VEGF ) bioactivity , resulted in virtually complete suppression of CL angiogenesis in a rat model of hormonally induced ovulation . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
The possible participation of VEGF and its high affinity tyrosine kinase receptors , flk 1 and flt 1 , in early background diabetic retinopathy was studied in the streptozotocin induced diabetic rat model of experimental retinopathy using in situ hybridization , blotting techniques , and immunohistochemistry . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Genomic organization of the flt 1 gene encoding for vascular endothelial growth factor ( VEGF ) receptor 1 suggests an intimate evolutionary relationship between the 7 Ig and the 5 Ig tyrosine kinase receptors . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
We found that the mRNA for the VEGF and VEGF C binding VEGFR 2 ( KDR / Flk 1 ) was stimulated by IL 1beta in human umbilical vein endothelial cells , whereas the mRNA levels of VEGFR 1 ( Flt 1 ) and VEGFR 3 ( Flt 4 ) were not altered . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Ang 2 itself had no effect on cell growth , tube formation , or mRNA levels of VEGF and tms like tyrosine kinase ( Flt 1 ) in BRECs . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Vascular endothelial growth factor ( VEGF ) , a major regulator of angiogenesis , binds to two receptor tyrosine kinases , KDR / Flk 1 and Flt 1 . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
The number of binding sites for VEGF decreased during DC maturation in vitro associated with decreased levels of mRNA for Flt 1 . ^^^ Thus , NF kappa B plays an important role in maturation of HPCs to DC , and VEGF activation of the Flt 1 receptor is able to block the activation of NF kappa B in this system . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Cultured RMC expressed the high affinity tyrosine kinase VEGF receptors , flt and flk / KDR , and the levels were not modulated by incubation in normal or high glucose media . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
On the basis of the knowledge that solid tumor growth beyond a small volume is critically dependent on angiogenesis , and that adenovirus ( Ad ) vectors can mediate efficient in vivo gene transfer and expression , we hypothesized that Ad mediated transfer of a secreted form of the extracellular domain of the flt 1 VEGF receptor ( Adsflt ) would suppress tumor growth on a regional basis . ^^^ We conclude that Ad mediated in vivo regional delivery of a secreted form of the extracellular domain of the flt 1 VEGF receptor can effectively inhibit regional tumor growth , a strategy that may provide a means to control tumor growth within the treated organ without the risk of systemic antiangiogenesis . . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
The endothelium was in most cases positive for bFGF , PDGF AA , PDGF BB , VEGF , PDGF alpha R , PDGF beta R , and TGF beta 1 but TGF beta / was negative in most cases and TGF alpha , EGFR , Flt 1 , Flk 1 / KDR , TGF beta R 1 , and TGF beta R 2 were variably expressed . ^^^ The most interesting possible auto / paracrine loops , as demonstrated on serial sections and by fluorescence double staining , were the TGF alpha / EGFR , TGF beta s / TGF beta R , VEGF / Flt 1 , and the VEGF / Flk 1 combinations . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Five growth factors and their receptors : platelet derived growth factor A chain ( PDGF AA ) and PDGF alpha receptor ( PDGF alpha R ) , PDGF BB and PDGF beta receptor ( PDGF beta R ) , transforming growth factor alpha ( TGF alpha ) and its receptor epidermal growth factor receptor ( EGFR ) , and vascular endothelial growth factor ( VEGF ) and its receptors ( Flt 1 and Flk 1 / KDR ; two growth inhibiting factors : transforming growth factor beta 1 ( TGF beta 1 ) and TGF beta 2 and their receptor couple TGF beta R 1 and TGF beta R 2 ; and basic fibroblast growth factor ( bFGF ) were stained in 45 cases of invasive breast cancer by standard immunohistochemistry on frozen sections . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Vascular endothelial growth factor ( VEGF ) binds to its receptor tyrosine kinase Flt 1 and KDR / Flk 1 and stimulates their autophosphorylation . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
A DNA fragment representing mRNA encoding the VEGF receptor , flt 1 , was identified in FRTL 5 cells by reverse transcription PCR analysis , and the abundance of this fragment was increased in FRTL 5 cells cultured in the medium containing TSH ( 1 mU / ml ) or fibroblast growth factor ( FGF ) 2 ( 25 ng / ml ) . ^^^ These results indicated that VEGF and one of its receptors , Flt 1 , are present in epithelial cells of the thyroid , and that VEGF could contribute to the regulation of development and function of thyroid epithelial cells . . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Fetal and newborn explants had a highly complex network of branched vessels that immunoexpressed the flt 1 VEGF receptor , and flk 1 VEGF receptor expression was determined by reverse transcription PCR . ^^^ VEGF infusions produced both a remarkable localized neovascularization and , unexpectedly , the expression of flt 1 on reactive astrocytes but not on endothelial cells . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
We provide evidence for a novel pregnancy associated soluble variant of the VEGF receptor Flt 1 . ^^^ Soluble Flt 1 may be important in regulating the actions of VEGF in angiogenesis and trophoblast invasion and may have therapeutic implications in diseases with inappropriate angiogenesis such as proliferative retinopathies and cancer . . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
In humans , the cellular actions of VEGF depend on binding to two specific receptors : Flt 1 and KDR . ^^^ The aims of this study were : ( 1 ) to localize VEGF receptor proteins in human renal ontogenesis ; ( 2 ) to quantify VEGF binding in human fetal and adult kidney ; and ( 3 ) to dissect the binding into its two known components : the KDR and Flt 1 receptors . ^^^ The latter aim was achieved by competitive binding of VEGF and placenta growth factor 2 , which only binds to Flt 1 . ^^^ A coexpression of both VEGF binding sites could be shown , with Flt 1 demonstrating the most abundant VEGF receptor binding sites in the kidney . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
In contrast , activation of VEGF receptor 1 ( Flt 1 ) by its specific ligand placenta growth factor ( PlGF ) had no effect on the tyrosine phosphorylation of cadherins and catenins . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
A paradox of Flt 1 , a tyrosine kinase receptor for vascular endothelial growth factor ( VEGF ) , is that the ligand can not activate the receptor to stimulate growth of cells that exogenously overexpress the receptor . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
The FLT 1 and KDR genes encode transmembrane tyrosine kinases which function as high affinity receptors for vascular endothelial growth factor ( VEGF ) . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
The binding of VEGF to its two known receptors , KDR and Flt 1 , is modulated by cell surface associated heparin like glycosaminoglycans and exogenous heparin or heparan sulfate . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
The aim of this study was to explore the possible androgenic regulation of the angiogenic factor vascular endothelial growth factor ( VEGF ) and its receptors flt 1 and flk 1 / KDR in the rat ventral prostate ( VP ) and Dunning R 3327 PAP adenocarcinoma . ^^^ VEGF , flt 1 , and flk 1 / KDR mRNA levels were determined using competitive RT PCR . ^^^ RESULTS : VEGF 121 , VEGF 165 , and VEGF 189 together with flt 1 and flk 1 / KDR mRNA were detected . ^^^ The VEGF , but not flt 1 mRNA levels were significantly decreased in the VP after castration . ^^^ The flt 1 mRNA level in the tumor increased after castration , while the VEGF mRNA levels were unchanged . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
These mice demonstrated an increased density of tortuous cutaneous blood capillaries with elevated expression levels of the high affinity VEGF receptors , VEGFR 1 and VEGFR 2 , most prominently during the neonatal period . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
The VEGF receptors , kinase domain region ( KDR ) and Fms like tyrosine kinase ( Flt 1 ) , also were upregulated in the tumor vasculature of glioblastoma multiforme , anaplastic oligodendrogliomas , and ependymomas with necrosis , whereas the astrocytomas grade 2 , anaplastic astrocytomas , and oligodendroglioma tumors tended to express a weak to nondetectable signal . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
RNAse protection from cells activated in vivo demonstrated biphasic induction of flt 1 and flk 1 mRNAs , receptors for vascular endothelial growth factor ( VEGF ) . ^^^ Culture activation of stellate cells was associated with increased [ 125I ] VEGF binding and Flt 1 and Flk 1 receptor protein . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
To block VEGF dependent angiogenesis , tumor cells were transfected with cDNA encoding the native soluble FLT 1 ( sFLT 1 ) truncated VEGF receptor which can function both by sequestering VEGF and , in a dominant negative fashion , by forming inactive heterodimers with membrane spanning VEGF receptors . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
The aptamers potently inhibit the binding of VEGF to the human VEGF receptors , KDR and Flt 1 , expressed by transfected porcine aortic endothelial cells . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Receptor tyrosine kinases Flt 1 and Flk 1 / KDR , and their ligand , the vascular endothelial growth factor ( VEGF ) , were shown to be essential for angiogenesis in the mouse embryo by gene targeting . ^^^ Flt 1 differs from Flk 1 in that it displays a higher affinity for VEGF but lower kinase activity , suggesting the importance of its extracellular domain . ^^^ However , VEGF induced macrophage migration was strongly suppressed in flt 1 ( TK / ) mice . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
It binds to the flt 1 VEGF receptor but not to the KDR / flk 1 receptor which is thought to mediate most of the angiogenic and proliferative effects of VEGF . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
In the present study , the localization and magnitude of VEGF , VEGF receptor 1 ( VEGFR 1 ) , and VEGF receptor 2 ( VEGFR 2 ) gene expression were examined in the eye of streptozotocin induced diabetic rats using quantitative in situ hybridization . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Vascular endothelial growth factor ( VEGF ) is a homodimeric glycoprotein that promotes angiogenesis and vascular hyperpermeability and interacts with two receptors , fms like tyrosine kinase ( Flt 1 ) and kinase domain containing region ( KDR ) . ^^^ In situ localization in the pregnant human uterus revealed that VEGF mRNA is expressed primarily by the maternal decidua , whereas the receptor Flt 1 is expressed primarily by chorionic vascular endothelium and trophoblast cells in particular , the extravillous trophoblast ( EVT ) . ^^^ Results of immunocytochemical and reverse transcription polymerase chain reaction analysis revealed that both protein and mRNA of VEGF , Flt 1 , and KDR were expressed by cultured normal EVT cells as well as their premalignant derivative produced by SV 40 Tag immortalization , and BeWo choriocarcinoma cells . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Expression of vascular endothelial growth factor ( VEGF ) and its receptors ( Flt 1 and Flk 1 ) following permanent and transient occlusion of the middle cerebral artery in the rat . ^^^ The present report describes the immunohistochemical distribution of VEGF and its 2 receptors , Flt 1 and Flk 1 at day 1 and 3 following permanent and transient middle cerebral artery occlusion ( MCAO ) in the rat . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
The effects of coronary artery disease ( CAD ) on human coronary microvascular responses to vascular endothelial growth factor ( VEGF ) and the alterations of the myocardial expressions of VEGF and its flk 1 and flt 1 receptors were examined in 48 patients . ^^^ CAD is associated with reduced vascular responses to both VEGF and HGF ; this is not likely due to a reduced expression of VEGF or flt 1 or flk 1 receptors and not due to a generalized endothelium dysfunction despite the presence of mild hypercholesterolemia in these patients with CAD . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
VEGF receptors 1 and 2 ( flt 1 and flk 1 ) as well as the recently identified angiopoietin receptors ( tie 1 and tie 2 ) are receptor tyrosine kinases specifically expressed in endothelial cells . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
VEGF stimulated activity in monocytes is mediated by the VEGF receptor flt 1 . ^^^ To explore the signal transduction pathway for the effect of VEGF on SMCs , we studied the expression of 2 high affinity VEGF receptors , the kinase insert domain containing receptor ( KDR ) and flt 1 , in human SMCs . ^^^ Immunoprecipitation followed by immunoblotting illustrated phosphorylation of the flt 1 receptor after VEGF treatment . ^^^ These data suggest the role of flt 1 in mediating VEGF stimulated MMP expression of SMCs . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Evidence for upregulation and redistribution of vascular endothelial growth factor ( VEGF ) receptors flt 1 and flk 1 in the oxygen injured rat retina . ^^^ In this study we probed for the two VEGF receptors ( VEGFRs ) known to have highest affinity in the rat flt 1 and flk 1 . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
These vascular defects resemble those in mice lacking the vascular specific endothelial cell growth factor VEGF or its receptor Flt 1 , both of which are expressed in MEF2C mutant embryos . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
These results indicate that Sck binds to KDR and Flt 1 via its SH 2 domain and might play an important role in VEGF signal transduction . . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
To elucidate the mechanisms of sinusoidal endothelial cell regeneration in vivo , mRNA expression of VEGF and its receptors , flt 1 and KDR / flk 1 , were studied in rat livers . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
METHODS : Non radioactive in situ hybridization was performed to examine the expression of VEGF mRNA and its receptors , flt 1 and KDR / flk 1 , in a rat model of nephrosis induced by intraperitoneal injection of bovine serum albumin ( BSA ) . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
The biological activities of VEGF in vivo and in its target cells in culture are mediated through two receptor protein tyrosine kinases , KDR / Flk 1 and Flt 1 . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
To further examine the mechanism of the potentiation of VEGF effect by rhEPO , we analyzed the mRNA expression of the VEGF receptors KDR / flk 1 and flt 1 . ^^^ The results disclosed that BAEC pretreatment with rhEPO upregulated the expression of both KDR / flk 1 and flt 1 , therefore providing a structural basis for the aforementioned positive interactions between VEGF and rhEPO . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Characterization of VEGF receptor ( VEGFR ) expression revealed that BME , BAE , and BLE cell lines express VEGFR 1 and 2 , whereas of the three cell lines assessed , only BAE cells express VEGFR 3 . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Furthermore , VEGF E bound KDR / Flk 1 ( VEGFR 2 ) and induced its autophosphorylation to almost the same extent as VEGF 165 , but did not bind Flt 1 ( VEGFR 1 ) nor induce autophosphorylation of Flt 1 . ^^^ Vascular endothelial growth factor ( VEGF ) mediates endothelial cell proliferation , angiogenesis , and vascular permeability via the endothelial cell receptors , KDR / Flk 1 and Flt 1 . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
VEGF expression as well as the expression of 2 VEGF receptors , flt 1 and Flk 1 , were studied with immunohistochemical techniques in these samples at the different stages of human coronary atherosclerosis progression . ^^^ CONCLUSIONS : These results demonstrate distinct expression of VEGF and its receptors ( flt 1 and Flk 1 ) in atherosclerotic lesions in human coronary arteries . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Archival specimens of 51 intestinal type and 38 diffuse type human gastric carcinomas were examined for tumor vessel counts , angiogenic factor expression , and the presence or absence of angiogenic factor receptors on tumor endothelium using antibodies against vascular endothelial growth factor ( VEGF ) and its receptors ( KDR and flt 1 ) , basic fibroblast growth factor ( bFGF ) and its receptors ( bek and flg ) , and factor 8 ( endothelial cells ) . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
We investigated expression of the vascular endothelial growth factor ( VEGF ) gene and its receptor gene ( flt 1 ) in 30 patients with transitional cell carcinoma ( TCC ) of the urinary bladder by Northern blot hybridization analysis . ^^^ The expression level of flt 1 gene significantly correlated with that of the VEGF gene in the tumor . ^^^ These results indicate that the VEGF gene is frequently overexpressed in TCC of the urinary bladder , especially in muscle invasive tumors , and that a paracrine system including VEGF and flt 1 exists between the TCC cells and the adjacent endothelial cells so as to regulate the angiogenesis in this tumor . . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Here we show by in situ hybridization that the trophoblast contains the mRNA encoding a soluble version of the VEGF receptor known as Flt 1 ( sFlt 1 : initially described by Kendall and Thomas , PNAS 90 : 10705 10709 ) . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
The diverse biological activities of VEGF are mediated through at least two receptor protein tyrosine kinases , KDR / Flk 1 and Flt 1 . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
To characterize molecular and cellular systems that may play a role in regulating blood vessel maturation , we have ( a ) analyzed the spatiotemporal expression of vascular endothelial growth factor ( VEGF ) and its receptors VEGF R 1 ( Flt 1 ) and VEGF R 2 ( Flk 1 ) throughout the ovarian cycle , ( b ) examined the recruitment of pericytes during vessel maturation , and ( c ) quantitatively measured the ratio of angiopoietin 2 ( Ang 2 ) to angiopoietin 1 ( Ang 1 ) throughout the ovarian cycle . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Intriguingly , the expression of VEGF receptor 1 ( VEGFR 1 ) was detected in certain spermatogenic cells in addition to vascular endothelium , and both VEGFR 1 and VEGFR 2 were also found in the Leydig cells of the testis . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Furthermore , in addition to VEGF itself , expression of VEGF receptor 1 ( VEGFR 1 ) , but not VEGFR 2 , was induced by hypoxia in endothelial cells of lung , heart , brain , kidney , and liver . ^^^ In these tissues , up or down regulation of VEGF and VEGFR 1 during hypoxia may influence their oxygenation after angiogenesis or modulate vascular permeability . . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
The various VEGF forms bind to two tyrosine kinase receptors , VEGFR 1 ( flt 1 ) and VEGFR 2 ( KDR / flk 1 ) , which are expressed almost exclusively in endothelial cells . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
VEGF mRNA and its receptor flt 1 are expressed in reactive astrocytes following neural grafting and tumor cell implantation in the adult CNS . ^^^ In addition , VEGF and its receptor flt 1 protein expression were detected immunohistochemically . ^^^ In all three models we found unexpectedly that only reactive astrocytes , not endothelium , expressed the VEGF receptor flt 1 , VEGF mRNA , and VEGF protein in a spatiotemporal manner , suggesting that activated astroglia may have a direct role in the induction of angiogenesis or permeability in mature brain . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Vascular endothelial growth factor ( VEGF ) and placental derived growth factor ( PlGF ) stimulate cell proliferation and differentiation by binding to their specific receptors , Flk 1 / KDR and Flt 1 respectively . ^^^ The aim of this study was to directly evaluate the importance of VEGF , PlGF / Flt 1 and Flk 1 / KDR receptor ligand interactions in regulating normal and malignant human haemopoiesis . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
This study aimed to investigate whether VEGF released from hypoxia exposed Hep G 2 cells alters expression of the two distinct receptors , kinase insert domain containing receptor ( KDR ) and fms like tyrosine kinase 1 ( flt 1 ) , in human umbilical venous endothelial cells ( HUVEC ) . ^^^ When the VEGF release from hypoxia exposed Hep G 2 cells was blocked by its antisense oligodeoxynucleotide , the endothelial flt 1 mRNA upregulation elicited by the hypoxia CM was still maintained . ^^^ These results suggest that hypoxia exposed Hep G 2 cells not only produce VEGF but also evolve paracrine induction of flt 1 through VEGF independent mechanisms . . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Peptides that inhibit binding of vascular endothelial growth factor ( VEGF ) to its receptors , KDR and Flt 1 , have been produced using phage display . ^^^ The peptides bind to a region of VEGF known to contain the contact surface for Flt 1 and the functional determinants for KDR binding . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
The interface between the peptide and VEGF contains a subset of the residues recognized by a neutralizing Fab fragment and overlaps partially with the binding site for the Flt 1 receptor . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Vascular endothelial growth factor ( VEGF ) and its specific receptors Flt 1 ( VEGFR 1 ) and KDR ( VEGFR 2 ) compose potent ligand receptor systems involved in angiogenesis and microvascular hyperpermeability . ^^^ Immunohistochemical localization of vascular endothelial growth factor ( VEGF ) and its two specific receptors , Flt 1 and KDR , in the porcine placenta and non pregnant uterus . ^^^ In the present immunohistochemical study , VEGF , Flt 1 and KDR were localized in uterus of cyclic non pregnant pigs and in the porcine epitheliochorial placenta throughout gestation . ^^^ In non pregnant pigs , VEGF , Flt 1 and KDR exhibited moderate to intense staining in uterine luminal epithelium and smooth muscle cells of the vessel walls . ^^^ In the fetal part of the placenta , VEGF , Flt 1 and KDR immunostaining displayed moderate to intense reactivity in the trophoblast throughout gestation , except during the second half of early gestation ( days 21 30 p . c . ) . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Five of the 12 cell lines were also found to express the Flt 1 receptor for VEGF at a moderate to strong level , suggesting an autocrine pathway . ^^^ Plasma cells in the bone marrow from patients diagnosed with multiple myeloma were found to express VEGF , whereas both the Flt 1 and KDR high affinity VEGF receptors were observed to be markedly elevated in the normal bone marrow myeloid and monocytic cells surrounding the tumor . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
We identified vascular endothelial growth factor ( VEGF ) and its receptor fms like tyrosine kinase ( FLT 1 ) to be under the regulatory control of exogenously applied NO in these cells . ^^^ Using S nitroso glutathione ( GSNO ) as an NO donating agent , VEGF expression was strongly induced , whereas expression of its FLT 1 receptor simultaneously decreased . ^^^ Expressional regulation of VEGF and FLT 1 mRNA was transient and occurred rapidly within 1 3 h after GSNO treatment . ^^^ The inflammatory cytokine interleukin 1beta mediated a moderate increase in VEGF expression after 24 h and had no influence on FLT 1 expression . ^^^ In contrast , platelet derived growth factor BB and basic fibroblast growth factor had no effect on VEGF expression , but strongly induced FLT 1 mRNA levels . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
In crossbreeding experiments , hemizygous ablation of the tumor suppressor genes Rb and p 53 had no significant effect on the expression of VEGF , flt 1 , flk 1 , tie 1 , and tie 2 . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
VEGF and PIGF exert their biological actions by means of a common receptor VEGFR 1 . ^^^ In the first trimester trophoblast cells , PIGF 1 increased the association of phosphorylated extracellular signal related kinase ( ERK ) with VEGFR 1 immunoprecipitates while both PIGF 1 and PIGF 2 also potentiated endogenous VEGF mediated association of phosphorylated extracellular related kinase ( ERK ) with VEGFR 2 ( KDR ) . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
VEGF is present in neuroblastoma but not vascular endothelial cells , whereas the corresponding VEGF receptors ( Flt 1 and Flk 1 / KDR ) are expressed in endothelial but not neuroblastoma cells . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
ORFV 2 VEGF was found to bind and induce autophosphorylation of VEGFR 2 and was unable to bind or activate VEGFR 1 and VEGFR 3 , but bound the newly identified VEGF 165 receptor neuropilin 1 . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Abundance of VEGF receptor mRNAs ( Flt 1 and KDR / Flk ) decreased in the late time points of the study ( 48 hours ) , possibly secondary to the loss of endothelial cells . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
VEGF , VEGFR 1 , VEGFR 2 , microvessel density and endothelial cell proliferation in tumours of the ovary . ^^^ VEGF , VEGFR 1 , VEGFR 2 and EC proliferation were examined immuno histochemically , and in situ hybridisation ( ISH ) studies of VEGF mRNA expression were performed and assessed in regions of high ( HVD ) and average ( AVD ) vessel density . ^^^ VEGF immunostaining did not differ between tumour types ; however , the percentage of VEGFR 1 and VEGFR 2 positive vessels was significantly lower in mucinous tumours . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Alternative splicing of vascular endothelial growth factor ( VEGF ) R 1 ( FLT 1 ) pre mRNA is important for the regulation of VEGF activity . ^^^ On day 11 of pregnancy , sFLT 1 mRNA was undetectable but FLT 1 readily apparent , and by day 17 sFLT 1 mRNA was abundant but FLT 1 barely detectable . sFLT 1 was identified in conditioned medium of cultured placenta from day 17 pregnant mice and likely to be present in the circulation , as there is a substantial increase of VEGF binding activity in the serum from day 13 of pregnancy , which coincides with the abundant sFLT 1 expression in placenta . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Treatment with either hypoxia or VEGF under normoxic conditions induced a twofold increase in VEGF binding sites and VEGF receptor 1 ( Flt 1 ) mRNA expression in BMEC . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
To investigate whether angiogenesis contributes to the pathogenesis of human colonic angiodysplasia , we examined the expression of basic fibroblast growth factor ( bFGF ) and vascular endothelial growth factor ( VEGF ) , and its endothelial cell receptors flt 1 and KDR . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
We also investigated the expression of vascular endothelial growth factor ( VEGF ) , placenta growth factor ( PlGF ) and their receptor FLT 1 . ^^^ These results suggest that KDR , FLT 1 , PlGF and TIE 1 mRNAs are present in the mesenchymal cells of RCC , while VEGF and FGFR 4 genes are expressed in RCC cells themselves in vivo . . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Castration induced regression and testosterone stimulated regrowth of the prostatic vasculature have here been used to study the involvement of the angiogenic factor vascular endothelial growth factor ( VEGF ) and its receptors flt 1 and flk 1 / KDR in the regulation of the prostatic vasculature . ^^^ MATERIALS AND METHODS : VEGF , flt 1 , and flk 1 / KDR levels were quantified in the rat ventral prostate following castration and testosterone replacement . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
The immunolocalization and gene expression of vascular endothelial growth factor ( VEGF ) and its cognate tyrosine kinase receptors , Flt 1 and KDR , has been studied in ocular melanomas and retinoblastomas using in situ hybridization and immunohistochemistry . ^^^ Flt 1 and KDR gene expression and immunolocalization occurred in VEGF expressing ganglion , Mller and amacrine cells in normal eyes . ^^^ Within the intra ocular tumours , VEGF receptor gene expression and protein was evident in the endothelial cells and also in cells close to the vessels , while in the adjacent retina , Flt 1 and KDR levels were elevated over normal , especially in the blood vessels . ^^^ This study suggests that VEGF , Flt 1 and KDR are expressed by neural , glial and vascular elements within normal human retina . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Fifth , pericytes expressed the gene for fms like tyrosine kinase 1 ( flt 1 ) as the predominant form of VEGF receptor , and tyrosine phosphorylation of this receptor protein was enhanced when pericytes were exposed to hypoxia , as it was when cells were exposed to VEGF . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
We obtained evidence that it is mainly vascular endothelial growth factor ( VEGF ) that mediates the angiogenic activity of insulin as follows . ( 1 ) Insulin upregulates the level of mRNA coding for secretory forms of VEGF , while the expression of the two VEGF receptor genes , kinase insert domain containing receptor ( kdr ) and fms like tyrosine kinase 1 ( flt 1 ) , was essentially unchanged by exposure to insulin . ( 2 ) A monoclonal antibody against human VEGF can completely neutralize both the proliferation and the tube formation of EC induced by insulin . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
If hepatic stellate cells can express FLT tyrosine receptor family , flt 1 and KDR / flk 1 , their function might be regulated by VEGF in an autocrine manner . ^^^ We conclude that VEGF may inhibit contraction of hepatic stellate cells appearing during activation by culture , probably through attenuation of smooth muscle alpha actin expression via upregulated VEGF receptor , flt 1 . . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
The aim of this study was to quantify and localize the mRNA expression of the vascular endothelial growth factor ( VEGF ) receptors Flt 1 , KDR and sflt , in human endometrium throughout the menstrual cycle . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Vascular endothelial growth factor B ( VEGF B ) is structurally closely related to VEGF and binds one of its receptors , VEGFR 1 . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
We found that the 2 VEGF receptors , KDR / Flk 1 and Flt 1 , were expressed in cardiac myocytes and that KDR / Flk 1 was significantly tyrosine phosphorylated on VEGF stimulation . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Data generated in our laboratory have shown that adenovirus mediated regional anti VEGF therapy using a gene encoding a soluble form of flt 1 ( one of the VEGF receptors ) can be used for regional control of tumor growth . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Production of VEGF and expression of the VEGF receptors Flt 1 and KDR in primary cultures of epithelial and stromal cells derived from breast tumours . ^^^ Production of vascular endothelial growth factor ( VEGF ) and expression of its receptors Flt 1 and KDR was determined in primary cultures of separated epithelial and stromal enriched cultures derived from ten primary human breast carcinomas . ^^^ Thus , production of VEGF and expression of VEGF receptors Flt 1 and KDR by breast cancer epithelial and stromal cells suggests that VEGF may fulfil not only an angiogenic role , but also play a fundamental role as an autocrine / paracrine regulator in breast cancer , thereby facilitating tumour proliferation and subsequent invasion . . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
To determine the role of VEGF in endochondral bone formation , we inactivated this factor through the systemic administration of a soluble receptor chimeric protein ( Flt ( 1 3 ) IgG ) to 24 day old mice . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Vascular endothelial growth factor ( VEGF ) and its receptors Flt 1 and KDR play important roles in physiological and pathological angiogenesis . ^^^ Ribozymes targeting Flt 1 or KDR mRNA sites reduced VEGF induced proliferation of cultured human vascular endothelial cells and specifically lowered the level of Flt 1 or KDR mRNA present in the cells . ^^^ Anti Flt 1 and KDR ribozymes also exhibited anti angiogenic activity in a rat corneal pocket assay of VEGF induced angiogenesis . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
We used the middle cerebral artery occlusion model ( MCAO ) in the rat to investigate VEGF mRNA and protein localization , and VEGFR 1 mRNA and VEGFR 2 mRNA expression in cerebral ischemia . ^^^ By nonradioactive in situ hybridization we observed upregulation of VEGF mRNA and VEGFR 1 mRNA , but not of VEGFR 2 mRNA in the hemisphere ipsilateral to MCA occlusion . ^^^ The spatial and temporal correlation between the induction of angiogenesis with VEGF and VEGFR 1 expression suggests that the ischemic upregulation of VEGF represents a physiological response of the brain to counterbalance hypoxia / ischemia in order to protect neuroectodermal tissue . . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Expression of vascular endothelial growth factor / vascular permeability factor ( VEGF / VPF ) and its receptors ( flt 1 and flk 1 ) during the peri implantation period ( days 3 , 4 , 5 , 6 and 7 post coitus ) in the golden hamster was investigated by in situ hybridization , immunohistochemistry and the reverse transcription / polymerase chain reaction ( RT PCR ) . ^^^ At day 4 , the subepithelial stroma and embryo displayed immunoreactivity for VEGF and flt 1 , whereas endothelial cells expressed both flt 1 and flk 1 . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
A375v human melanoma cells , which express at least the angiogenic growth factors VEGF , VEGF C , and Ang 1 , were stably transfected to overexpress the extracellular ligand binding domains of the endothelium specific receptor tyrosine kinases fms like tyrosine kinase 1 ( Flt 1 ) , Flt 4 , Tie 1 , and Tie 2 , respectively . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Expression of vascular endothelial growth factor ( VEGF ) and its corresponding receptors ( flt 1 and flk 1 ) in the bovine oviduct . ^^^ Reverse transcription polymerase chain reaction ( RT PCR ) and ribonuclease protection assay ( RPA ) were used to show that the bovine oviduct expresses VEGF and its two receptors flk 1 and flt 1 . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Vascular endothelial growth factor B ( VEGF B ) is expressed in various tissues , especially strongly in the heart , and binds selectively to one of the VEGF receptors , VEGFR 1 . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
METHODS : Normal and inflamed pleura were examined immunohistochemically for the presence of FLT 1 ( the fms like tyrosine kinase receptor of VEGF ) . ^^^ RESULTS : Immunohistochemical staining of normal and diseased pleura demonstrated the presence of the FLT 1 VEGF receptor on human mesothelial cells . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
To investigate the role of vascular endothelial growth factor ( VEGF ) in fibrogenesis , the distribution patterns of the VEGF receptors Flt 1 and Flk 1 were studied by immunohistochemistry , double immunofluorescence , and immunoelectron microscopy in normal ( n=2 ) and bleomycin treated ( n=21 ) adult rats . ^^^ Flt 1 , Flk 1 , and VEGF immunoreactivity localised predominantly to the pulmonary epithelium . ^^^ In control lungs , Flt 1 immunoreactivity was present in ciliated bronchial epithelium and type 2 pneumocytes , Flk 1 in Clara cells , and VEGF in Clara cells and type 2 pneumocytes . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Osteoclasts predominantly expressed VEGF receptor 1 ( VEGFR 1 ) , and activity of recombinant human placenta growth factor 1 on osteoclast recruitment was comparable to that of rhVEGF , showing that the VEGF signal is mediated through VEGFR 1 . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
The expression of VEGF and its two receptors , Flk 1 and Flt 1 , is pivotal for the proper formation of blood vessels in embryogenesis as shown by gene targeting experiments . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Immunolocalisation of the VEGF receptors FLT 1 , KDR , and FLT 4 in diabetic retinopathy . ^^^ AIM : To determine the spatial and temporal changes in the staining pattern of the VEGF receptors FLT 1 , KDR , and the putative receptor FLT 4 during the pathogenesis of diabetic retinopathy . ^^^ METHODS : Immunohistochemical localisation of VEGF receptors , using antibodies against FLT 1 , FLT 4 , and KDR , was carried out on specimens of normal human retina ( n = 10 ) , diabetic retinas ( a ) with no overt retinopathy ( n = 12 ) , ( b ) with intraretinal vascular abnormalities but no proliferative retinopathy ( n = 5 ) , ( c ) with active proliferative retinopathy ( n = 6 ) , and ( d ) with no residual proliferative retinopathy after scatter photocoagulation therapy ( n = 14 ) , and surgically excised diabetic fibrovascular membranes ( n = 11 ) . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
To interfere with the action of VEGF , a nuclease stabilized ribozyme , ANGIOZYME , has been developed against VEGF receptor subtype Flt 1 mRNA . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Constitutive expression of VEGF , VEGFR 1 , and VEGFR 2 in normal eyes . ^^^ PURPOSE : The expression of vascular endothelial growth factor ( VEGF ) and its high affinity receptors VEGFR 1 and VEGFR 2 was investigated in normal eyes . ^^^ Ocular VEGF , VEGFR 1 , and VEGFR 2 expression was studied using a combination of in situ hybridization , northern blot analysis , immunohistochemistry , immunoassay , and reverse transcription polymerase chain reaction . ^^^ CONCLUSIONS : VEGF , VEGFR 1 , and VEGFR 2 are constitutively expressed in the vascularized tissues of normal eyes . . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Quantitative reverse transcription polymerase chain reaction analysis reveals a time and dose dependent up regulation of mRNA for VEGF receptors ( KDR and flt 1 ) . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
In addition , all cell lines and primary tumours expressed the mitogenic VEGF receptor FLK 1 , whilst the non mitogenic receptor FLT 1 was less frequently positive , suggesting that the tyrosine kinase FLK 1 is involved in malignant transformation of neuroblastoma cells . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Reverse transcriptase polymerase chain reaction analysis demonstrated the presence of both KDR and flt mRNA , two known VEGF receptors , in SMC cultures . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
The mRNA levels for both types of VEGF receptors , Flk 1 and Flt 1 were low but detectable in FRC cells by RT PCR and were not changed by OP 1 . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
VEGF binds to two cell surface tyrosine kinase receptors , KDR ( kinase domain region ) and Flt 1 ( fms like tyrosine kinase 1 ) . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
The HEEC were very responsive to VEGF growth stimulation likely due to elevated affinity , or increased levels of , KDR and FLT 1 on the cell surface . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Characterization of the VEGF binding site on the Flt 1 receptor . ^^^ The angiogenic growth factor VEGF binds to the receptor tyrosine kinases Flt 1 and KDR / Flk 1 . ^^^ Immunoglobulin ( Ig ) like loop 2 of Flt 1 is involved in binding VEGF , but the contribution of other Flt 1 Ig loops to VEGF binding remains unclear . ^^^ We tested the ability of membrane bound chimeras between the extracellular domain of Flt 1 and the cell adhesion molecule embigin to bind VEGF . ^^^ VEGF bound as well to receptors containing Flt 1 loops 1 2 or 2 3 as it did to the entire Flt 1 extracellular domain . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
VEGF exerts its angiogenic and pro tumorigenic properties by way of two high affinity receptors , fms like tyrosine kinase 1 ( FLT 1 ) and fetal liver kinase 1 ( FLK 1 ) . ^^^ RESULTS : PCa cells expressed the VEGF receptor FLT 1 in 100 % of specimens evaluated . ^^^ The expression of FLT 1 and FLK 1 on tumor cells themselves suggests a potential autocrine function for VEGF ( such as regulating tumor cell proliferation ) . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
To investigate the role of VEGF in maintaining the absorptive function of the intramembranous microvessels , the present study was undertaken to determine the gestational change in gene expression of VEGF and its receptors , kinase insert domain containing receptor ( KDR ) and fms like tyrosine kinase ( Flt 1 ) , in ovine placenta , chorion , and amnion . ^^^ The relative abundance of VEGF , KDR , and Flt 1 mRNA was determined by Northern blot analysis , and VEGF molecular forms expressed were identified by reverse transcriptase polymerase chain reaction . ^^^ In the placenta , KDR was the primary VEGF receptor expressed , although Flt 1 was also detected at very low levels . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
The percentages of tumours with higher expression compared to the mean values of normal pleural tissues were 31 . 5 % ( 17 / 54 ) for VEGF , 66 . 7 % ( 36 / 54 ) for VEGF C , 20 . 4 % ( 11 / 54 ) for fms like tyrosine kinase ( flt ) 1 , 42 . 6 % ( 23 / 54 ) for kinase insert domain containing recepter ( KDR ) and 59 . 3 % ( 32 / 54 ) for flt 4 . ^^^ Significant positive correlations were found between VEGF C and flt 4 , VEGF and KDR , VEGF and flt 1 in tumour tissues . ^^^ There were also significant correlations between LVD and flt 4 , and VD and VEGF . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Finally , we investigated the expression of both VEGF tyrosine kinase receptors ( Flt 1 and Flk 1 / KDR ) in tumor specimens by RT PCR . ^^^ Expression of ( Flt 1 and Flk 1 / KDR ) was low in tissue specimens derived from NPA tumors , but was found enhanced in NPA VEGF tumors ; conversely , the expression of VEGF receptors was high in tissue specimens derived from ARO tumors but was decreased in tumors derived from VEGF depleted ARO cells . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
VEGF via VEGF receptor 1 ( Flt 1 ) mimics preeclamptic plasma in inhibiting uterine blood vessel relaxation in pregnancy : implications in the pathogenesis of preeclampsia . ^^^ Vascular endothelial growth factor ( VEGF ) is an angiogenic growth factor that acts via two high affinity receptors ( KDR and Flt 1 ) , and its production is increased in preeclampsia . ^^^ Here we report that VEGF and its Flt 1 receptor may play a pivotal role in the altered vascular function of preeclampsia . ^^^ However , in the presence of an anti Flt 1 receptor antibody , VEGF or plasma from women with preeclampsia no longer attenuated the endothelium dependent relaxation ( p < 0 . 05 ) . ^^^ The changes observed with VEGF and plasma from women with preeclampsia and their subsequent blockade with anti VEGF antibody and anti Flt 1 receptor antibody strongly suggest that VEGF acting through the Flt 1 receptor is pivotal in the pathogenesis of this disease . . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Levels of Tie 2 , angiopoietin ( Ang ) 1 , vascular endothelial growth factor ( VEGF ) , and CD 31 mRNAs were higher in cancers than in adjacent noncancerous tissues , in contrast to the fms like tyrosine kinase ( Flt ) 1 , Flt 4 , Tie 1 , thrombin receptor , endoglin , and VEGF C , for which no differences were evident . ^^^ Significant positive correlations were found between mRNA expression of Ang 1 and those of Tie 2 and CD 31 , and that of VEGF and those of Flt 1 and CD 31 . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
The expression of vascular endothelial growth factor ( VEGF ) and its receptors Flt 1 and Flk 1 in the rat kidney was examined during ontogeny using Northern blot analysis and immunocytochemistry . ^^^ In prevascular embryonic kidneys ( embryonic day 14 [ E 14 ] ) , immunoreactive Flt 1 and Flk 1 were observed in isolated angioblasts , whereas VEGF was not detected . ^^^ In late fetal kidneys ( E 19 ) , immunoreactive VEGF was detected in glomerular epithelial and tubular cells , whereas Flt 1 and Flk 1 were expressed in contiguous endothelial cells . ^^^ VEGF induced upregulation of Flk 1 and Flt 1 expression , as assessed by Western blot analysis . ^^^ Flt 1 , Flk 1 , and angiotensin converting enzyme containing cells were detected in VEGF treated explants , whereas control explants were negative . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Apart from the expected expression of VEGF receptors on endothelial cells we observed a tumor cell specific localization of FLT 1 in 29 tumors and KDR in 16 of 37 tumors analyzed . ^^^ Our data suggest a potential negative regulatory loop for VEGF and FLT 1 when tumor cells have an insufficient blood supply . . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
VEGF is expressed in glioma cells and its receptors ( Flt 1 and KDR ) are expressed in the same gliomas . ^^^ KDR is a receptor for the various VEGF isoforms and for VEGF C ; Flt 1 is a receptor for the various isoforms . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Consequently , we sought to evaluate by antisense knockdown of gene expression the contribution of VEGF receptors ( Flt 1 and Flk 1 ) on these events . ^^^ The capacity to inhibit the protein synthesis of Flt 1 and Flk 1 by antisense oligonucleotides provides a new approach to block VEGF pathological effects in vivo . . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
A specific receptor for VEGF , fms like tyrosine kinase ( Flt 1 ) , is expressed in vascular endothelial cells that mediates the action of VEGF . ^^^ We examined the localization and expression of VEGF and Flt 1 , using an immunohistochemical technique and RT PCR analysis , in human follicles and corpora lutea during the normal menstrual cycle and early pregnancy . ^^^ RT PCR analysis demonstrated that messenger ribonucleic acids encoding VEGF 121 , VEGF 165 , and Flt 1 were expressed in the CL . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
We examined VEGF mRNA expression , VEGF isoform pattern and VEGF receptor ( flt 1 and KDR ) by RT PCR analysis in 30 osteosarcomas . ^^^ All 30 osteosarcomas expressed VEGF mRNA . 17 osteosarcomas ( 57 % ) expressed flt 1 mRNA , whilst 20 ( 67 % ) expressed KDR mRNA . 6 / 30 ( 20 % ) osteosarcomas were positive for VEGF 121 only , 8 ( 27 % ) for VEGF 121 + VEGF 165 , and 16 ( 53 % ) for VEGF 121 + VEGF 165 + VEGF 189 . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
The effects of VEGF are mediated mainly through two distinct receptors , flt 1 and KDR / Flk 1 . ^^^ In a xenograft study , tumor augmentation induced by VEGF overexpression was almost abolished by means of KDR / Flk 1mAb treatment , with accompanying inhibition of angiogenesis , KDR / Flk 1 autophosphorylation , but not interference of flt 1 activation . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
The expression patterns of vascular endothelial growth factor ( VEGF ) and its two receptors , flt 1 and KDR , were assessed in normal human melanocytes , transformed melanocytes expressing the simian virus 40 Tgene ( SV40T ) , and melanoma cells derived from primary and metastatic lesions . ^^^ Constitutive expression of VEGF , flt 1 , and KDR mRNA and proteins was observed in the majority of primary and metastatic melanoma cell lines , and in SV40T transformed melanocytes . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
The extracellular portion of the VEGF and PlGF receptor , Flt 1 ( or VEGFR 1 ) , consists of seven immunoglobulin like domains . ^^^ Solution structure of the VEGF binding domain of Flt 1 : comparison of its free and bound states . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
The fourth immunoglobulin like loop in the extracellular domain of FLT 1 , a VEGF receptor , includes a major heparin binding site . ^^^ To examine whether FLT 1 , a VEGF receptor , interacts with heparin and which domain of FLT 1 binds to heparin , various extracellular domains of FLT 1 were expressed in a baculovirus / insect cell system : sFLT 1 ( 1 7 ) , sFLT 1 ( 1 4 ) , sFLT 1 ( 1 3 ) , and sFLT 1 ( 1 2 ) containing immunoglobulin ( Ig ) like loop one to seven , one to four , one to three , and one to two , respectively . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Endothelial cell tyrosine kinase receptors , KDR and Flt 1 , have been implicated in VEGF responses including cellular migration , proliferation , and modulation of vascular permeability . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Furthermore , VEGF treatment increased eNOS expression in a KDR but not in an Flt 1 receptor transfected porcine aorta endothelial cell line . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
The VEGF receptors fms like tyrosine kinase 1 ( Flt 1 ) / VEGFR 1 and kinase insert domain containing receptor ( KDR ) / VEGFR 2 are up regulated on the surface of endothelial cells ( ECs ) in gliomas . ^^^ These properties prompted us to investigate Ets 1 expression in 32 human astroglial tumors of WHO grades 1 4 and to correlate the data with the expression pattern of VEGF , Flt 1 , and KDR . ^^^ Our data suggest that VEGF secreted by glioma cells induces Ets 1 in adjacent microvascular ECs , which subsequently transactivates the VEGF receptor Flt 1 . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Vascular endothelial growth factor ( VEGF ) is a potent angiogenic polypeptide that activates 2 distinct high affinity tyrosine kinase receptors , flk 1 / KDR and flt 1 . ^^^ In the present study , we characterized the expression of VEGF and its receptors flk 1 / KDR and flt 1 in the normal human pancreas and in human pancreatic cancer tissues and cell lines . ^^^ VEGF , flk 1 / KDR and flt 1 mRNA levels were elevated in cancer tissues compared with normal pancreas . ^^^ By immuno histochemistry , VEGF , flk 1 / KDR and flt 1 immunoreactivity co localized in many of the cancer cells within the tumor mass . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Reduced tissue oxygen tension triggers VEGF expression , and increased protein and mRNA levels for VEGF and its receptors ( Flt 1 , Flk 1 / KDR ) occur in the ischemic rat brain . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Reverse transcription polymerase chain reaction analysis demonstrated the presence of VEGF receptor mRNA ( flt 1 and KDR ) in HMCs . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Using immunohistochemistry , the results demonstrated that the tissue recombinants typically exhibited an overexpression of EGF , EGF R , bFGF , TGF beta ( 1 ) together with a concurrent downregulation of TGF alpha , IGF 1 , IGF 2 , and VEGF receptors ( flk 1 , flt 1 ) . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Co expression of vascular endothelial growth factor ( VEGF ) and its receptors ( flk 1 and flt 1 ) in hormone induced mammary cancer in the Noble rat . ^^^ The action of VEGF is mediated by two high affinity receptors with ligand stimulated tyrosine kinase activity : VEGFR 1 / flt 1 and VEGFR 2 / flk 1 , which are expressed mainly in vascular endothelial cells . ^^^ Intense reactivities to VEGF , flt 1 and flk 1 were observed in mammary cancer cells , especially in invasive mammary carcinoma . ^^^ To further verify this hypothesis , we investigated the correlation between cellular proliferation and the expression of VEGF , flt 1 and flk 1 . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
There was differential expression of the VEGF receptors , Flt 1 and Flk 1 , in hypoxic T 6 cells . ^^^ Hypoxia stimulates cell signaling in stellate cells , culminating in the rapid induction of VEGF and Flt 1 mRNA expression and VEGF secretion . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Vascular endothelial growth factor ( VEGF ) is one of the key factors in tumor neoangiogenesis , acting through its receptors KDR ( VEGFR 2 ) and fit 1 ( VEGFR 1 ) expressed on endothelial cells . ^^^ Concerning the functional significance of VEGF receptor expression , VEGF treatment of VEGFR 1 expressing tumor cells induced the inhibition of cell proliferation by 25 to 55 % and the inhibition of tumor cell migration by 29 to 55 % . ^^^ Thus our data indicate that the coexpression of VEGF and VEGFR 1 in tumor cells could have an inhibitory effect on tumor cell proliferation and migration , a mechanism possibly induced as a response to a deficiency in nutrient and oxygen supply . . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
The spatial and temporal immunoexpression of the intermediate filament ( IF ) protein nestin and its relationship to glial fibrillary acidic protein ( GFAP ) , vascular endothelial growth factor ( VEGF ) , and its receptor flt 1 ( VEGF R 1 ) in reactive astroglia was examined following stab wounds or transplants of fetal CNS tissue into the adult brain . ^^^ Nestin colocalized with VEGF in some astrocytes ( 7 % ) but far more prominently with the VEGF flt 1 receptor ( 25 % ) . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
VEGF , flt 1 , and KDR / flk 1 as prognostic indicators in endometrial carcinoma . ^^^ Vascular endothelial growth factor ( VEGF ) , an important regulator of tumor angiogenesis in the endometrium , flt 1 , and KDR / flk 1 are good markers of vascular proliferation . ^^^ Being that angiogenesis is a precursor to the development of progressive disease , we hypothesize that quantifying VEGF , flt 1 , and KDR / flk 1 expression in uterine malignancies is a superior predictor of metastatic potential and survival than is FIGO grade of tumor , depth of invasion , and histology . ^^^ Immunohistochemistry staining was performed for VEGF , flt 1 , and KDR / flk 1 . ^^^ The impact of VEGF , flt 1 , and KDR / flk 1 as well as stage , grade , depth of invasion , and nodal status on the incidence of metastases , recurrence , and survival was determined using logistic regression analysis and product limit life system survival analysis , respectively . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
A series of substituted 4 anilinoquinazolines and related compounds were synthesized as potential inhibitors of vascular endothelial growth factor ( VEGF ) receptor ( Flt and KDR ) tyrosine kinase activity . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
We conducted the present study to determine , by immunocytochemistry and computerized image analysis of the functionalis , the expression and modulation of the receptors Flk 1 / KDR and Flt 1 , which mediate VEGF effects on endothelial mitogenicity , chemotaxis , and capillary permeability . ^^^ Finally , strong expression of Flt 1 , but not Flk 1 / KDR , was observed on dilated capillaries during the premenstrual period and the late proliferative phase , suggesting preferential association of Flt 1 with nonproliferating capillaries at those times ; activation of this receptor by VEGF could be involved in premenstrual vascular hyperpermeability , edema , and extravasation of leukocytes . ^^^ We conclude that Flt 1 and Flk 1 / KDR in the functionalis are modulated in parallel or independently according to the phase of the cycle , and that these changes are responsible for VEGF actions on endometrial vascular growth and permeability . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
The VEGF receptor 1 ( Flt 1 ) shows the same alternative splicing in humans and dogs and is overexpressed in the majority of tumors in both species . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Immunohistochemical detection of vascular endothelial growth factor ( VEGF ) and VEGF receptors Flt 1 and KDR / Flk 1 in the vestibule of guinea pigs . ^^^ We performed immunohistochemical analysis using specific antibodies to VEGF and to both known VEGF receptors Flt 1 and KDR / Flk 1 on paraffin sections of temporal bones from guinea pigs ( n=5 ) . ^^^ Immunoreactivity of VEGF , Flt 1 and KDR / Flk 1 was detectable in a subpopulation of vestibular ganglion cells . ^^^ These findings give evidence that VEGF , Flt 1 and KDR / Flk 1 are constitutively expressed in the vestibule . . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Compared with controls , the MA and BO groups showed : ( 1 ) diminished placental trophoblastic VEGF immunoreactivity ; ( 2 ) weaker VEGFR 1 and 2 immunoreactivity in decidual vascular endothelium ; ( 3 ) reduced placental trophoblastic Tie 1 receptor immunoreactivity ; and ( 4 ) reduced decidual vascular endothelial Tie 1 and 2 receptor immunoreactivity . ^^^ Interestingly , placental villi from the BO group presented blood vessel like structures negative for von Willebrand factor , but positive for VEGF , VEGFR 1 , 2 , 3 , Tie 1 and Tie 2 receptor . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
In addition to its mitogenic activities , VEGF has also been found to stimulate migration in macrophages via the flt 1 VEGF receptor . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Angiogenic and vasculogenic factors include VEGF , which signals through cognate receptors flk and flt , while novel anti angiogenic factors include EMAP II . . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Coexpression of VEGF and its receptor flt 1 has been reported in different types of malignant tumors . ^^^ OBJECTIVE : In the present study we investigated the expression of VEGF and flt 1 in 90 cases of diffuse malignant pleural mesotheliomas . ^^^ METHODS : VEGF and flt 1 expression was analyzed by immunohistochemistry and non radioactive in situ hybridization . ^^^ Results : VEGF expression was visualized immunohistochemically in tumor cells . flt 1 expression correlated with histological differentiation ( p < 0 . 013 ) . ^^^ VEGF and flt 1 expression were confirmed by in situ hybridization . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
ZD 4190 , a substituted 4 anilinoquinazoline , is a potent inhibitor of KDR and Flt 1 RTK activity , and VEGF stimulated HUVEC proliferation in vitro . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Seventeen of 52 leukemia and lymphoma cell lines investigated expressed sFlt 1 mRNA , and 16 of the sFlt 1 expressing cells also expressed VEGF and membrane bound Flt 1 ( mFlt 1 ) . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
METHODS : We assessed amniotic fluid from the second ( n = 35 , gestational weeks 14 19 ) and third ( n = 29 ) trimesters of healthy women , and from the third trimesters of preeclamptic ( n = 22 ) and diabetic women with ( n = 11 ) or without preeclampsia ( n = 34 ) and from women with fetal growth restriction ( FGR ) ( n = 14 ) for soluble VEGF receptor 1 ( VEGFR 1 ) by enzyme linked immunosorbent assay . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Vegf , Vegf B , Vegf C and their receptors KDR , FLT 1 and FLT 4 during the neoplastic progression of human colonic mucosa . ^^^ Because the crucial role of angiogenesis has been demonstrated in tumor growth and metastasis , the present study was undertaken to characterize the relative expression of vascular endothelial growth factors VEGF ( vascular endothelial growth factor ) , VEGF B , VEGF C , and their receptors KDR ( kinase insert domain containing receptor ) , FLT 1 ( fms like tyrosine kinase ) , and FLT 4 in human colonic cancers , in relation to the Astler Coller pathological classification , and to prognosis . ^^^ In contrast to KDR and FLT 4 , the expression of VEGF C and FLT 1 genes increased in some pathological tissues . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Using porcine endothelial cells expressing one or the other type of the VEGF receptors , VEGFR 1 or VEGFR 2 , or human umbilical vein endothelial cells pretreated with a VEGFR 2 neutralizing antibody , we show that VEGFR 2 is responsible for VEGF induced activation of the stress activated protein kinase 2 / p38 ( SAPK2 / p38 ) , phosphorylation of focal adhesion kinase ( FAK ) , and enhanced migratory activity . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Although VEGF receptor 1 ( VEGFR 1 ) and VEGFR 2 are known to be high affinity receptors for VEGF , it is not clear which of the VEGFRs are responsible for the transmission of the diverse biological responses of VEGF . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Homeostatic modulation of cell surface KDR and Flt 1 expression and expression of the vascular endothelial cell growth factor ( VEGF ) receptor mRNAs by VEGF . ^^^ Regulation of the receptor tyrosine kinases , KDR and Flt 1 , to which VEGF binds on endothelial cells is incompletely understood . ^^^ Chronic incubation with tumor conditioned medium or VEGF diminished ( 125 ) 1 VEGF binding to human umbilical vein endothelial cells , incorporation of ( 125 ) 1 VEGF into covalent complexes with KDR and Flt 1 , and immunoreactive KDR in cell lysates . ^^^ Preincubation with VEGF or tumor conditioned medium down regulated cell surface receptor expression but up regulated KDR and Flt 1 mRNAs , an effect abrogated by a neutralizing VEGF antibody . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Despite much progress in recent years , the precise signalling events triggered by the vascular endothelial growth factor ( VEGF ) receptors , fms like tyrosine kinase ( Flt 1 ) and kinase insert domain containing receptor ( KDR ) , are incompletely defined . ^^^ It has also been difficult to demonstrate VEGF induced phosphorylation of Flt 1 , which has led to speculation that KDR may be the more important receptor for the mitogenic action of VEGF on endothelial cells . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Overexpression of VEGF 121 in immortalized endothelial cells causes conversion to slowly growing angiosarcoma and high level expression of the VEGF receptors VEGFR 1 and VEGFR 2 in vivo . ^^^ The angiosarcomas generated from MS 1 VEGF cells demonstrated up regulation of the VEGF receptors VEGFR 2 and VEGFR 1 ( Flt 1 ) in vivo compared with benign hemangiomas generated from MS 1 cells . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
As shown by immunocytochemistry or RT PCR , myeloma cells did not express or weakly expressed the VEGF receptors FLT 1 and FLK 1 / KDR , indicating that autocrine stimulation is unlikely . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
It mediates its activity mainly via two tyrosine kinase receptors , VEGFR 1 ( flt 1 ) and VEGFR 2 ( flk 1 / KDR ) , although other receptors , such as neuropilin 1 and 2 , can also bind VEGF . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
MATERIALS AND METHODS : We have investigated the expression ( 4 grade values , blinded assay by two observers ) of NOS 1 3 together with those of VEGF , VEGF R ( Flt 1 ) , EGF R 1 , von Willebrand factor ( VWF ) and a pan macrophage marker ( Ki M1P ) immunohistochemically in tumor specimens from 220 patients and performed tumor volume morphometry by image analysis in a subgroup of 32 cases to test for any correlation with the peritumoral oedema volumes . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
MATERIALS AND PATIENTS : Cryosections ( N = 96 ) of metastatic resections were investigated immunohistologically using a 4 step grading evaluation for the expression of NOS 1 3 , VEGF receptor FLT 1 , a pan macrophage marker Ki M1P , and capillary vessel presence by endothelial Von Willebrand Factor staining . ^^^ The VEGF receptor FLT 1 could be detected in 70 % of them , 24 % in higher expression 2 and 3 . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
We constructed an adenovirus ( AdVEGF ExR ) expressing the entire extracellular domain of the human vascular endothelial growth factor ( VEGF ) receptor ( flt 1 ) fused to the Fc portion of human IgG . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
VEGF functions via binding to the VEGF receptors Flk 1 and Flt 1 . ^^^ We hypothesized that this might occur via reduced VEGF , TGF beta ( 1 ) , bFGF , Flk 1 , and Flt 1 gene expression at rest and after exercise . ^^^ VEGF , TGF beta ( 1 ) , bFGF , Flk 1 , and Flt 1 mRNA were analyzed from the left gastrocnemius by quantitative Northern blot . ^^^ Exercise increased VEGF mRNA 4 . 8 fold , TGF beta ( 1 ) mRNA 1 . 6 fold , and Flt 1 mRNA 1 . 7 fold but did not alter bFGF or Flk 1 mRNA measured 1 h after exercise . ^^^ Captopril did not affect the rest or exercise levels of VEGF , TGF beta ( 1 ) , bFGF , and Flt 1 mRNA . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
We examined the relationships between VEGF , the type 1 VEGF receptor ( FLT 1 ) and clinical outcome for a group of thyroid cancers in children and young adults . ^^^ The expression of VEGF and FLT 1 were determined by immunohistochemistry using archival , paraffin embedded thyroid tissue blocks and compared with the retrospective clinical outcome for each patient . ^^^ FLT 1 expression was greater in PTC ( mean intensity 2 . 8 + / 0 . 17 ) than FTC ( 1 . 9 + / 0 . 25 , p = 0 . 015 ) and benign lesions ( 1 . 7 + / 0 . 32 , p = 0 . 002 ) ; and correlated with PTC size ( r = 0 . 41 , p = 0 . 01 ) as well as VEGF expression ( r = 0 . 52 , p = 0 . 002 ) . ^^^ We conclude that the expression of VEGF and FLT 1 are directly correlated with the size of PTC in children and young adults . . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Two distinct VEGF receptors , KDR / Flk 1 and Flt 1 , were detectable in osteoclasts at the gene and protein levels , and VEGF induced tyrosine phosphorylation of proteins in osteoclasts . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Roles of two VEGF receptors , Flt 1 and KDR , in the signal transduction of VEGF effects in human vascular endothelial cells . ^^^ VEGF expresses its effects by binding to two VEGF receptors , Flt 1 and KDR . ^^^ However , properties of Flt 1 and KDR in the signal transduction of VEGF mediated effects in endothelial cells ( ECs ) were not entirely clarified . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Biologically active VEGF was expressed along a time course that paralleled the expression of two specific VEGF receptors , Flk 1 and Flt 1 , and the progression of joint disease . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Two VEGF tyrosine kinase receptors have been reported fms like tyrosine kinase 1 ( Flt 1 ) and kinase domain region ( KDR ) . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Regulation of placental vascular endothelial growth factor ( VEGF ) and placenta growth factor ( PIGF ) and soluble Flt 1 by oxygen a review . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
OBJECTIVE : The aim of this study has been to evaluate the clinical significance of expression of VEGF and its receptors , Flt 1 , KDR , and Flt 4 , in endometrial carcinomas . ^^^ The overall positive rates in the 86 carcinoma specimens were 66 % for VEGF , 51 % for Flt 1 , 38 % for KDR , and 57 % for Flt 4 . ^^^ Survival curves determined by the Kaplan Meier method and univariate analysis showed VEGF , Flt 1 , and Flt 4 overexpression to be related to poor prognosis of patients with endometrial carcinomas . ^^^ However , multivariate analysis revealed that Flt 4 overexpression correlated independently with poor survival , similar to a value for myometrial invasion at one half or more and that for retroperitoneal lymph node metastasis , whereas VEGF and Flt 1 overexpression did not . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Vascular endothelial growth factor ( VEGF ) acts primarily as an endothelial cell mitogen via the `` endothelial cell specific ' ' receptors VEGFR 1 ( flt 1 ) and VEGFR 2 ( flk 1 / KDR ) . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
To confirm the specificity of these observations , mice were pretreated with intranasal administration of an adenovirus vector expressing a truncated soluble form of the VEGF receptor flt 1 ( Adsflt ) . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
In addition , exogenous soluble human Flt 1 used as an inhibitor of endogenous VEGF binding also inhibited ascites recurrence . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Here we report in vitro binding experiments that shed light on the interaction between VEGF 165 and NP 1 , as well as a previously unknown interaction between NP 1 and one of the VEGF receptor tyrosine kinases , VEGFR 1 or Flt 1 . ^^^ We also show that the NP 1 ECD binds with high affinity ( K ( d ) = 1 . 8 nm ) to domains 3 and 4 of Flt 1 and that this interaction inhibits the binding of NP 1 to VEGF 165 . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Flt 1 ( VEGF receptor 1 ) and KDR / Flk 1 ( VEGF receptor 2 ) are the high affinity receptors for the angiogenesis factor , vascular endothelial growth factor ( VEGF ) . ^^^ These results suggest that there would be an angiogenesis mechanism via VEGF / Flt 1 or VEGF / KDR in HCC , and the VEGF / KDR system would take a more important role . . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Vascular endothelial growth factor ( VEGF ) , a potent mitogen , is expressed in podocytes in the glomerulus , and VEGF receptors ( flt 1 , KDR , and neuropilin 1 ) are present on endothelial cells and other cell types . ^^^ In conclusion , flt 1 , KDR , and neuropilin 1 are present on cultured HMC , and VEGF ( 165 ) induces HMC proliferation . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Properties of two VEGF receptors , Flt 1 and KDR , in signal transduction . ^^^ The properties of two VEGF receptors , Flt 1 and KDR , in the signal transduction of VEGF in human umbilical vein endothelial cells ( HUVECs ) were investigated by using two newly developed blocking monoclonal antibodies ( mAbs ) against Flt 1 and KDR . ^^^ VEGF stimulated the expression of transcription factor Ets 1 as well as matrix metalloproteinase 1 ( MMP 1 ) and Flt 1 in HUVECs . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
The aim of the present study was to evaluate the effectiveness of gene therapy against cancer , including peritoneal metastasis , using a cDNA encoding a soluble type of Flt 1 , one of the VEGF receptors . ^^^ In conclusion , this study demonstrated that the peritoneal metastases of some cancers were largely dependent on VEGF , and that the repeated intraperitoneal transduction of a soluble flt 1 gene using HVJ cationic liposomes suppressed peritoneal metastases , thereby contributing to a longer survival period . . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Vascular endothelial growth factor ( VEGF ) is a pleiotropic factor that exerts a multitude of biological effects through its interaction with two receptor tyrosine kinases , fms like tyrosine kinase ( Flt 1 ) or VEGF receptor 1 and kinase insert domain containing receptor ( KDR ) or VEGF receptor 2 . ^^^ Based on a comprehensive mutational analysis of the receptor binding site of VEGF , an Flt 1 selective variant was created containing four substitutions from the wild type protein . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
A superimposition of this dimer onto VEGF in its complex with the receptor Flt 1 places the binding sites on SCF in positions of topographical and electrostatic complementarity with the Kit counterparts of Flt 1 , and a similar model can be made for the complex of PDGF with its receptor . . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
VEGF receptors , the tyrosine kinases Flt 1 and KDR , are expressed on vascular endothelial cells and initiate angiogenesis upon activation by VEGF . 1 Anilino ( 4 pyridylmethyl ) phthalazines , such as CGP 79787D ( or PTK 787 / ZK 222584 ) , reversibly inhibit Flt 1 and KDR with IC ( 50 ) values < 0 . 1 microM . ^^^ Modification of the 1 anilino moiety afforded derivatives with higher selectivity for the VEGF receptor tyrosine kinases Flt 1 and KDR compared to the related receptor tyrosine kinases PDGF R and c Kit . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
In the present study , the expression of vascular endothelial growth factor ( VEGF ) , an endothelial cell specific angiogenic mitogen , and its receptors , fms like tyrosine kinase ( Flt 1 ) and kinase insert domain containing receptor ( KDR ) , in the lungs of five cases with PH , were examined . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Plasma VEGF and soluble VEGF receptor FLT 1 in proliferative retinopathy : relationship to endothelial dysfunction and laser treatment . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
VEGF receptor ( VEGFR 1 and 2 ) expression was determined using reverse transcription polymerase chain reaction and fluorescence activated cell sorting , and affinity was measured using Scatchard analysis . ^^^ Pretreatment with TGF beta 2 ( 10 ng / ml ) was associated with increased expression of the VEGFR 1 in RPE cells and increased receptor affinity for VEGF detected by Scatchard analysis . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Expression of the VEGF receptor Flt 1 in benign , premalignant and malignant prostate tissues . ^^^ PURPOSE : Vascular endothelial growth factor ( VEGF ) is one of the most potent regulators of angiogenesis and has been shown to act upon two tyrosine kinase family receptors : c fms like tyrosine kinase ( Flt 1 ) and fetal liver kinase . ^^^ RESULTS : VEGF receptor Flt 1 specific anti sera revealed significant staining of prostatic endothelial cells , but the reactivity was not restricted to endothelial cells . ^^^ CONCLUSIONS : Widespread distribution of VEGF receptor Flt 1 in BPH , PIN and prostate cancer specimens suggests that VEGF function in prostate is not restricted to endothelial cells and angiogenesis . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
The potential acute toxicity of a ribozyme ( ANGIOZYME ) targeting the flt 1 vascular endothelial growth factor ( VEGF ) receptor mRNA was evaluated in cynomolgus monkeys following i . v . infusion or s . c . injection . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
This study examined the expression of vascular endothelial growth factor ( VEGF ) isoforms and VEGF receptors , Flt 1 , KDR and neuropilin 1 , in RA and osteoarthritis ( OA ) synovia , and studied the relationship between their expression and the synovial angiogenesis . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
The addition of acidic Fibroblast Growth Factor ( aFGF ) , basic FGF ( bFGF ) or Vascular Endothelial Growth Factor ( VEGF ) substantially improved proliferation of TdMEC ; and kidney carcinoma derived endothelial cells were more responsive to FGFs , whereas glioblastoma derived endothelial cells greatly responded to VEGF TdMEC expressed high levels of the VEGF receptors , KDR / flk 1 and Flt 1 , as shown by northern blot analysis . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Vascular endothelial growth factor ( VEGF ) has two highly homologous tyrosine kinase receptors : Flt 1 ( VEGFR 1 ) and KDR ( VEGFR 2 ) . ^^^ KDR is strongly phosphorylated on tyrosines and can transmit mitogenic and motogenic signals following VEGF binding , while Flt 1 is markedly less effective in mediating such functions . ^^^ When the juxtamembrane region of Flt 1 is replaced by that of KDR , Flt 1 becomes competent to mediate endothelial cell migration and phosphatidylinositol 3 ' kinase activation in response to VEGF . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
The aim of our present study was to investigate the expression of p125FAK and the coexpression with PYK2 / CAK beta , VEGF receptor FLT 1 , NOS isozymes NOS 1 3 , capillary density and the histology in 130 specimens of resected cerebral metastatic tumours . ^^^ MATERIALS AND METHODS : Cryosections ( N = 130 ) of metastatic resections were investigated immunohistologically using a 4 step scoring evaluation for the expression of NOS 1 3 , VEGF receptor FLT 1 , and capillary vessel presence by endothelial Von Willebrand Factor ( VWF ) staining . ^^^ The VEGF receptor FLT 1 could be detected in 70 % of them , 24 % at higher expression values 2 and 3 . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
After 1 week in culture the cells showed a clear expression of endothelial cell markers , including von Willebrand factor ( vWF ) , CD 144 ( VE cadherin ) , CD 105 ( endoglin ) , acetylated low density lipoprotein ( AC LDL ) receptor , CD 36 ( thrombospondin receptor ) , FLT 1 , which is vascular endothelial cell growth factor ( VEGF ) receptor 1 , and , to a weaker extent , KDR ( VEGF receptor 2 ) . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
METHODS : An adenovirus vector encoding a soluble VEGF receptor / flt 1 ( Adflt ExR ) was constructed . ^^^ CONCLUSIONS : Adenovirus mediated gene transfer of a soluble form of VEGF receptor ( flt 1 ) gene inhibited the growth of the experimental eyelid malignant melanoma . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
PURPOSE : To help further define the possible role of vascular endothelial growth factor ( VEGF ) in the pathogenesis of corneal neovascularization , the expression of VEGF and of its receptors Flt 1 and Flk 1 was investigated in various inflammatory corneal diseases . ^^^ Expression of both VEGF receptors , Flt 1 and Flk 1 , was increased on endothelial cells of newly formed vessels in the stroma of inflamed corneas compared with limbal vessels of normal control corneas . ^^^ CONCLUSIONS : These results demonstrate that VEGF , Flt 1 , and Flk 1 are strongly expressed in inflamed and vascularized human corneas and , thus , may play an important role in corneal neovascularization . . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Expression of another VEGF receptor flt 1 was consistent in all cells including HUVECs , HUVECs / E6 E 7 and HUVECs / E6 E7 / ras . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Like VEGF , it is a potent angiogenic factor ; however , PlGF homodimers interact with the VEGF receptor Flt 1 ( fms like tyrosine kinase ) , but not with the kinase domain containing region ( KDR ) . ^^^ Expression of mRNA for the receptors of VEGF and PlGF ( KDR and flt 1 ) was determined using the reverse transcriptase polymerase chain reaction . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
We investigated the properties of two VEGF receptors , Flt 1 and KDR , by using two newly developed blocking monoclonal antibodies ( mAbs ) , i . e . , anti human Flt 1 mAb and anti human KDR mAb . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
To block VEGF activity , animals were treated after arthritis onset with a soluble form of the Flt 1 VEGF receptor ( sFlt ) , which was polyethylene glycol ( PEG ) linked to increase its in vivo half life . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Vascular endothelial growth factor ( VEGF ) intracellular signaling in endothelial cells is initiated by the activation of distinct tyrosine kinase receptors , VEGFR 1 ( Flt 1 ) and VEGFR 2 ( Flk 1 / KDR ) . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
To investigate the mechanism of neuroprotection by VEGF , the expression of known target receptors for VEGF was measured by Western blotting , which showed that HN 33 cells expressed VEGFR 2 receptors and neuropilin 1 , but not VEGFR 1 receptors . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
At the molecular level , brief exposure of endothelial cells to thrombin causes an upregulation of the receptors ( KDR and Flt 1 ) of VEGF , the key angiogenic mediator . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
VEGF also enhanced tube formation in dog endothelial cells and increased the expression of two VEGF receptors , Flt 1 and KDR / Flk 1 . ^^^ VEGF also increases the expression of the receptors , KDR and Flt 1 , and activates the p44 / 42 MAP kinase pathway . . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Vascular endothelial growth factor ( VEGF ) and its receptors , VEGF receptor 1 ( VEGF R 1 ; FLT 1 ) and VEGF R 2 ( KDR ) , have been shown to play a major role in tumor angiogenesis . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
VEGF and its receptors flk 1 and flt 1 are the main actors of the morphogenic phase whereas PDGF BB and its receptor PDGF R beta , TGF beta 1 and its receptors ALK 5 and ALK 1 , angiopoietin 1 and its receptor tie 2 are implied in the vascular maturation phase . . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
VEGF receptor 2 [ VEGFR 2 ( KDR / Flk 1 ) ] mediates endothelial cell mitogenesis and permeability increases , whereas the role of VEGF receptor 1 [ VEGFR 1 ( Flt 1 ) ] has not been clearly defined . ^^^ In the present study , a monoclonal antibody , 2C3 , is shown to block the interaction of VEGF with VEGFR 2 but not with VEGFR 1 through ELISA , receptor binding assays , and receptor activation assays . 2C3 blocks the VEGF induced vascular permeability increase in guinea pig skin . 2C3 has potent antitumor activity , inhibiting the growth of newly injected and established human tumor xenografts in mice . ^^^ These findings demonstrate the usefulness of 2C3 in dissecting the pathways that are activated by VEGF in cells that express both VEGFR 1 and VEGFR 2 , as well as highlighting the dominant role of VEGFR 2 in mediating VEGF induced vascular permeability increase and tumor angiogenesis . . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
VEGF activated the tyrosine receptor phosphorylation of KDR and Flt 1 and increased the binding of phosphatidylinositol 3 kinase ( PI 3 kinase ) p 85 subunit to KDR and Flt 1 , both of which could mediate CTGF gene induction . ^^^ These data suggest that VEGF can increase CTGF gene expression in bovine retinal capillary cells via KDR or Flt receptors and the activation of PI 3 kinase Akt pathway independently of PKC or Ras ERK pathway , possibly inducing the fibrosis observed in retinal neovascular diseases . . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Using immunohistochemical and in situ hybridization methods we have here demonstrated that VEGF and its receptors Flk 1 , Flt 1 and Neuropilin 1 mRNAs and proteins are induced after incisions in the rat spinal cord . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Although BREC expressed Flt 1 and Flk 1 as VEGF receptors at similar levels , VEGF stimulation preferentially enhanced the activity of Flt 1 tyrosine kinase . ^^^ These results demonstrate that , in addition to Ras / MAPK pathways , the Flt 1 / Tyk2 / Stat6 pathway is important in VEGF signaling in BREC . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Raised levels of plasma vascular endothelial growth factor ( VEGF ) are found in some cancers , diabetes , and certain other conditions , but levels of its receptor , soluble Flt 1 ( sFlt 1 ) , in these diseases have yet to be reported . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
By inserting VEGF receptor Flt 1 ( 1 3 loop ) cDNA into Pichia pastoris expression vector pPIC9K containing AOX 1 promotor and the sequences of alpha secreting signal peptides , the expression plasmid pPIC9K / Flt 1 ( 1 3 ) was constructed and transformed into GS 115 . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
METHODS : VEGF , KDR / flk 1 , and flt 1 expression were examined by immunohistochemistry , in situ hybridization , reverse transcription polymerase chain reaction , enzyme linked immunosorbent assay , and receptor phosphorylation . ^^^ VEGF expression was observed in all human pancreatic tumor cell lines examined , and the KDR / flk 1 and flt 1 receptor was detected in 2 cell lines . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Vascular endothelial growth factor ( VEGF ) , through activation of its endothelial receptors VEGFR 1 and VEGFR 2 , is an important positive modulator of tumor angiogenesis and edema in solid tumors such as malignant astrocytomas . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Immunohistochemistry revealed that VEGF was localized in luteal cells and both flt 1 and KDR were also localized in luteal cells , in addition to vascular endothelial cells . ^^^ Messenger RNA ( mRNA ) expression of VEGF , flt 1 , and KDR remained constant in the CL during the luteal phase and was lower in the regression phase . ^^^ In the pregnant CL , VEGF mRNA expression was higher compared with that in the midluteal phase , and mRNA expression of both flt 1 and KDR was the same as that in the midluteal phase . ^^^ Western blot analyses revealed that the change in protein expression of VEGF , flt 1 , and KDR was similar to that in their mRNA expression . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
OBJECTIVE : We wanted to study whether free or bound VEGF is present in human milk and whether it and its receptors ( VEGFR 1 and 2 ) are expressed in lactating breast or newborn intestinal tissue . ^^^ VEGFR 1 and 2 immunoreactivity was seen in the glandular epithelial cells of the newborn intestine and lactating breast , whereas VEGF was present only in breast glandular epithelium . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Vascular endothelial growth factor ( VEGF ) specific Flk 1 and Flt 1 tyrosine phosphorylation was observed at Day 4 . 5 by receptor immunoprecipitation and requisite immunoblotting . ^^^ Likewise , neither VEGF ( 5 10 10 ( 4 ) to 10 ( 15 ) M ) nor PlGF ( 10 ( 6 ) to 10 ( 8 ) M ) , which selectively binds Flt 1 , served to increase FITC Dextran 40 efflux at Day 5 . 0 . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
The angiogenesis / vasculogenesis pathway is required for embryonic survival and includes several receptors ( VEGFR 1 , VEGFR 2 , Tie 2 ) and ligands ( VEGF , Ang 1 , Ang 2 , neuropillin ) . ^^^ VEGFR 1 may be primarily responsible for binding of VEGF to yolk sac and embryonic tissues , as masking VEGFR 2 did not reduce VEGF binding in those areas , and it is interesting that major structural defects were also not found in those regions . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
We report here the expression and modulation of VEGF and its receptors , Flk 1 / KDR and Flt 1 , in the functionalis throughout the menstrual cycle . ^^^ The localization of VEGF on the endothelium correlates with the presence of Flt 1 and Flk 1 / KDR receptors on vascular structures , including capillary strands that have not yet formed a lumen and that have been previously described in tumors as angiogenic capillaries . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
METHODS : VEGF , kinase domain region ( KDR ) / fetal liver kinase 1 ( flk 1 ) , and flt 1 expression were examined by immunohistochemistry and in situ hybridisation in 15 human HCC tissues . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
The TNX binding site of VEGF B was located in the N terminal 115 amino acid region . mTNX / FNIII13 25 did not prevent the interaction of VEGF B with VEGFR 1 ( VEGF receptor 1 ) , and VEGF B could simultaneously bind to both mTNX / FNIII13 25 and VEGFR 1 . ^^^ A conditioned medium from transfected 293T cells coexpressing full length TNX and VEGF B could promote DNA synthesis in bovine endothelial cells in which VEGFR 1 were expressed . ^^^ VEGFR 1 phosphorylation triggered by VEGF B 186 were increased in cells plated with mTNX / FNIII13 25 or full length mTNX , compared with cells plated with VEGF B 186 alone . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
The VEGF family members PIGF and VEGF B with exclusive binding capacities to the VEGFR 1 can influence monocyte activation and differentiation . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Vascular endothelial growth factor ( VEGF ) and its receptors VEGFR 1 and VEGFR 2 , fibroblast growth factor 2 ( FGF 2 ) and its receptors FGFR 1 and FGFR 2 , as well as epidermal growth factor ( EGF ) and its receptor EGFR are believed to be important in the control of angiogenesis in the human endometrium . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Vascular development in the implants was evaluated by mRNA expression of Flt 1 and KDR , two receptors for vascular endothelial growth factor ( VEGF ) . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Endothelial growth is influenced by a variety of soluble factors , and several of these are regulated by oxygen , for example , vascular endothelial growth factor ( VEGF ) , angiopoietin 2 , and soluble flt ( a VEGF antagonist ) . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Here we show that VEGF receptor 1 ( VEGFR 1 ) negatively regulates VEGFR 2 mediated proliferation via nitric oxide ( NO ) in an epithelial cancer cell line ECV 304 . ^^^ In contrast , VEGF ( 50 ng / ml ) stimulated NO release in a manner that was inhibited by functionally neutralizing anti VEGFR 1 antibody . ^^^ Cell cycle analysis showed that inhibition of VEGFR 1 increased the transition from G ( 1 ) to S phase whereas inhibition of VEGFR 2 blocked the VEGF mediated transition from G ( 1 ) to S phase . ^^^ Finally , the addition of NO donors suppressed both VEGF mediated proliferation and the increase in growth after blockade of VEGFR 1 . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Flt 1 , also known as vascular endothelial growth factor receptor 1 ( VEGFR 1 ) , is a high affinity tyrosine kinase receptor for VEGF and is expressed almost exclusively on vascular endothelial cells . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
The pharmacokinetics and tolerability of a chemically stabilized synthetic ribozyme ( ANGIOZYME ) targeting the Flt 1 VEGF receptor mRNA were evaluated in healthy volunteers . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
The expression of the signalling VEGF receptors R 1 ( flt 1 ) and R 2 ( flk 1 ) was restricted to the endothelial cells of the cortex whereas neuropilin 1 was expressed by both endothelial and steroidogenic cells . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Measurement of VEGF B together with other angiogenic factors may identify a poor prognostic patient group , which may benefit from anti VEGF receptor therapy targeted to flt 1 ( VEGFR 1 ) as well as kdr ( VEGFR 2 ) . . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
The molecular regulation of these distinct mechanisms is discussed in respect to the most important positive regulators , vascular endothelial growth factor ( VEGF ) and its receptors flk 1 ( KDR ) and flt 1 , the Angiopoietin / tie system and the ephrin B / EpH B system . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
The expression of VEGF and flt in the specimen of laryngeal carcinoma were determined by standardized immunohistochemistry ( Elite ABC method ) . ^^^ The immunohistochemical staining showed that the expressions of VEGF and flt were detected both in carcinoma cells and endothelial cells . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Because systemic hypertension increases vascular stretch , we evaluated the expression of VEGF , VEGF R 2 ( kinase insert domain containing receptor [ KDR ] ) , and VEGF R 1 ( fms like tyrosine kinase [ Flt ] ) in bovine retinal endothelial cells ( BRECs ) undergoing clinically relevant cyclic stretch and in spontaneously hypertensive rat ( SHR ) retina . ^^^ VEGF reacted similarly , but Flt expression did not change . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
SU 5416 , a potent inhibitor of the VEGF receptor 1 ( VEGFR 1 ) and VEGFR 2 receptor tyrosine kinases , did not abolish the inhibitory effect of VEGF , indicating that the VEGF effect is mediated by a mechanism unrelated to VEGFR 1 or VEGFR 2 tyrosine kinase activity . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
To elucidate the sequence of molecular events intricate with angiogenesis and the initiation and progression prostate cancer , the temporal and spatial expression patterns of platelet endothelial cell adhesion molecule 1 ( PECAM1 / CD31 ) , hypoxia induced factor 1alpha ( HIF 1alpha ) , vascular endothelial growth factor ( VEGF ) , and the cognate receptors VEGFR 1 and VEGFR 2 were characterized . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
VEGF interacts with the endothelium via two membrane spanning receptors , fms like tyrosine kinase ( Flt ) 1 and kinase domain receptor . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Recently VEGF B and VEGF C , two new VEGF family members , have been identified that bind to the tyrosine kinase receptors flt 1 ( VEGFR 1 ) , KDR ( VEGFR 2 ) , and flt 4 ( VEGFR 3 ) . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
No significant differences in the in vitro or in vivo expression of angiogenesis associated genes ( flt 1 , flt 4 , TIE 1 , TIE 2 , and VEGF ) was found between K7M2 and K 12 . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Various growth factors and their receptors , such as transforming growth factor alpha ( TGF alpha ) , heparin binding epidermal growth factor like growth factor ( HB EGF ) , TGF beta 2 , TG beta 3 , insulin like growth factor ( IGF ) 1 and 2 , vascular endothelial growth factor ( VEGF ) , EGF receptor ( EGFR ) , erb B 4 , TGF betaRI and 2 , Flt and Flk 1 and IGF receptor Ibeta , were found in epithelial cells and / or in some lymphoid cells . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Having recently shown increased expression of cellular VEGF in the leukemic cells of patients with chronic lymphocytic leukemia ( CLL ) we decided to investigate the expression of angiogenic receptors Flt 1 and Tie 1 . ^^^ Levels of Flt 1 but not Tie 1 correlated with levels of cellular VEGF . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
The three loci , designated Mmtg 1 3 , are unlinked to the angiogenic genes Fgf 2 , Flt 1 , Flk 4 , Flk 1 , Vegf , and Vegfc , as well as the precursors of the endogenous antiangiogenic molecules angiostatin and endostatin . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Vascular endothelial growth factor ( VEGF ) and its receptors , Flt 1 and flk 1 ( KDR ) , constitute an important angiogenic pathway which , under hypoxic conditions , is up regulated in many solid tumours . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
METHOD : We examined immunohistochemically expressions of VEGF and its corresponding receptors Flt 1 and Flk 1 in a series of 50 astrocytic tumours . and correlated their expressions with the degree of angiogenesis , brain edema and prognosis . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
The immunohistological analysis of the leptin treated rat cornea showed definitive rises in Ob R , MMPs and TIMPs expression as well as those of VEGF receptor ( VEGFR 1 ) . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
We studied the expression of VEGF and its receptors Flt 1 and KDR / Flk 1 mRNA by Northern and that of VEGF protein by Western blot . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
METHODS : Expression of VEGF protein from the synovial tissue of 10 patients with RA was monitored by ELISA and visualized by immunocytochemistry , and by double staining with the VEGFR 1 / flt 1 . ^^^ RESULTS : VEGF protein was strongly increased in rheumatoid synovium and localized at the synovial surface , whereas the VEGF receptor flt 1 ( VEGFR 1 ) was visualized on microvessels in close vicinity . ^^^ The presence of VEGF 121 may explain induction of the VEGFR 1 on infiltrating blood vessels near the synovial surface . . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
In this study , we tested whether vascular smooth muscles cells ( VSMCs ) can express VEGF receptors , such as flk 1 , flt 1 , and neuropilin ( NP ) 1 , and respond to VEGF in vitro . ^^^ Since VEGF E is a specific activator of flk 1 while PlGF specifically activates only flt 1 , SMC migration induced by VEGF ( 165 ) is likely to be mediated primarily through the flk 1 receptor . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Previous studies have demonstrated the expression of VEGF and its receptors , flt 1 or KDR / flk 1 , in hemangiosarcomas by immunohistochemical staining and in situ hybridization . ^^^ In the present study , however , we demonstrated that tumor cells of the hemangiosarcoma cell line ISO HAS express mRNA of VEGF and its two receptors , flt 1 and KDR , and secrete VEGF protein . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
The expression of genes coding for beta galactosidase ( beta gal ) , enhanced green fluorescent protein ( EGFP ) , vascular endothelial growth factor ( VEGF ) , and soluble Flt 1 ( s Flt ) was recorded and measured with regard to dose , time course , and bioactivity . ^^^ Moreover , corneal injection of plasmid containing the cDNA for the soluble form of the VEGF receptor Flt 1 effectively prevented corneal neovascularization . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
VEGF receptor tyrosine kinases , KDR / Flk 1 and Flt 1 , have been thought to be expressed exclusively by endothelial cells . ^^^ Analysis of ( 125 ) 1 VEGF ( 165 ) binding to NbE 1 and FB 2 cells indicated that only motile FB 2 cells expressed the VEGF receptor Flt 1 . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Additionally , we summarize the properties of 2C3 , a novel monoclonal anti VEGF antibody that blocks VEGF from binding to VEGFR 2 but not VEGFR 1 . 2C3 may be utilized as both an anti angiogenic agent by inhibiting VEGFR 2 activity and potentially as a vascular targeting agent by binding to blood vessels that express the VEGF VEGFR 1 complex . . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
We examined the regulation of the expression of vascular endothelial growth factor ( VEGF ) and its specific receptors , fetal liver kinase receptor ( Flk 1 ) , and fms like tyrosine kinase receptor ( Flt 1 ) during formation of the capillary network in the developing rat lung . ^^^ Immunostaining also revealed the colocalization of VEGF , Flt 1 , and Flk 1 in endothelial cells of the lung capillaries at the ultrastructural level . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Endothelial nitric oxide synthase is strongly expressed in malignant mesothelioma but does not associate with vascular density or the expression of VEGF , FLK 1 or FLT 1 . ^^^ AIMS : To investigate endothelial nitric oxide synthase ( eNOS ) expression in malignant mesothelioma and its association with expression of vascular endothelial growth factor ( VEGF ) , its receptors FLK 1 and FLT 1 , and vascular density . ^^^ METHODS AND RESULTS : eNOS , VEGF , FLK 1 and FLT 1 were studied in 36 histological mesothelioma samples by immunohistochemistry . ^^^ VEGF , FLK 1 and FLT 1 expression was found in 17 ( 45 % ) , 24 ( 69 % ) and 25 ( 71 % ) cases , respectively . ^^^ No significant association was found between FLK 1 , FLT 1 , VEGF and eNOS immunoreactivity and vascular density . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Two VEGF receptors , KDR ( kinase domain region ) and Flt 1 ( fms like tyrosine kinase ) are co expressed by glomerular and peritubular capillary endothelial cells . ^^^ In the present work we focused on the tubulo interstitial compartment ; by reverse transcription / polymerase chain reaction ( RT / PCR ) we evaluated the expression of VEGF , KDR , Flt 1 and the relationship between the two main type of VEGF isoforms , VEGF 121 and VEGF 165 in the tubulo interstitium of type 2 diabetic patients . ^^^ VEGF 121 isoform exceed VEGF 165 while Flt 1 was significantly lower in glomeruli . ^^^ CIII patients had the lowest tubulo interstitial level of VEGF and Flt 1 mRNAs . ^^^ Furthermore , at least in CIII patients , down regulation of the VEGF Flt 1 system could be involved in the mechanisms leading to tubulointerstitial diabetic lesions . . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Therefore , we examined the effects of exercise training ( treadmill running at the same absolute intensity ) in normoxia and hypoxia ( inspired O ( 2 ) fraction = 0 . 12 ) on rat skeletal muscle capillarity and on resting and postexercise gene expression of VEGF , its major receptors ( flt 1 and flk 1 ) , TGF beta ( 1 ) , and bFGF . ^^^ Rats trained in hypoxia exhibited an attenuated VEGF mRNA response to exercise ( 1 . 8 fold compared 3 . 4 fold with normoxic training ; P < 0 . 05 ) , absent TGF beta ( 1 ) and flt 1 mRNA responses to exercise , and an approximately threefold ( P < 0 . 05 ) decrease in bFGF mRNA levels . flk 1 mRNA levels were not significantly altered by either normoxic or hypoxic training . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
MATERIALS AND METHODS : Tumors from 45 patients were assessed by immunohistochemistry for expression patterns of five growth factors and their receptors ; platelet derived growth factor A chain ( PDGF AA ) and PDGF receptor alpha ( PDGFalphaR ) , PDGF BB and PDGFbetaR , transforming growth factor alpha ( TGFalpha ) and its receptor epidermal growth factor receptor ( EGFR ) and vascular endothelial growth factor ( VEGF ) and its receptors vascular endothelial growth factor receptor 1 ( FLT 1 ) and vascular endothelial growth factor receptor 2 ( FLK 1 / KDR ) , two growth inhibiting factors ; transforming growth factor beta 1 ( TGFbeta 1 ) and TGFbeta 2 and their receptor couple TGFbeta receptor 1 ( TGFbetaR 1 ) and TGFbetaR 2 , and basic fibroblast growth factor ( bFGF ) . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Vascular endothelial growth factor ( VEGF ) is known to be a highly specific mitogen for endothelial cells through two high affinity tyrosine kinase receptors , VEGFR 1 and VEGFR 2 , which are almost specifically expressed in endothelial cells . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
In the present study , we have expressed human VEGF receptors in insect cells using the baculovirus expression system , identified a major autophosphorylation site on the VEGF receptor fms like tyrosine kinase 1 ( Flt 1 ) by HPLC electrospray ionization ( ESI ) MS , and characterized in vitro interactions between Flt 1 and phosphatidylinositol 3 ' kinase ( PI 3 kinase ) . ^^^ Infection of High 5 insect cells with Flt 1 recombinant virus resulted in the expression of a 170 kDa glycoprotein , which bound VEGF with a K ( d ) of 2 10 10 ( 10 ) M in intact insect cells . ^^^ These results suggest that phosphorylated Flt 1 associates with PI 3 kinase at pTyr ( 1213 ) to mediate the activation of this pathway in VEGF signalling . . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Moreover , enhanced endothelial cell proliferation induced by conditioned medium of macrophages isolated from the ascites of patients with SBP is abolished by anti VEGF antibody , and peritoneal tissue of cirrhotic patients expresses both VEGF receptors , Flt 1 and KDR . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
However , it is unknown whether VEGFR 1 ( Flt 1 ) is an inert decoy or a signaling receptor for VEGF during physiological or pathological angiogenesis . ^^^ Here we report that VEGF stimulated nitric oxide ( NO ) release is inhibited by blockade of VEGFR 1 and that VEGFR 1 via NO negatively regulates of VEGFR 2 mediated proliferation and promotes formation of capillary networks in human umbilical vein endothelial cells ( HUVECs ) . ^^^ VEGF stimulated NO release from VEGFR 1 but not VEGFR 2 transfected endothelial cells and placenta growth factor 1 stimulated NO release in HUVECs . ^^^ Blockade of VEGFR 1 increased VEGF mediated HUVEC proliferation that was inhibited by NO donors , and potentiated by NO synthase inhibitors . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
We recently have shown that certain leukemias not only produce VEGF but also selectively express functional VEGF receptors ( VEGFRs ) , such as VEGFR 2 ( Flk 1 , KDR ) and VEGFR 1 ( Flt 1 ) , resulting in the generation of an autocrine loop . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Although SPARC derived peptide showed an angiogenic effect , intact SPARC itself inhibited the mitogenic activity of vascular endothelial growth factor ( VEGF ) for ECs by the inhibiting phosphorylation of flt 1 ( VEGF receptor 1 ) and subsequent ERK activation . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
More interestingly , all of the cell lines examined were found to express the mRNA and protein for fms like tyrosine kinase 1 ( Flt 1 ) , which is one of the receptors for VEGF . ^^^ In clinical specimens , expression of VEGF and Flt 1 mRNAs was detected in all ( 100 % ) of 11 and 8 ( 73 % ) of 11 ATL patients , respectively . ^^^ Cytofluorometric analysis revealed that VEGF effectively bound only to Flt 1 expressing cells . ^^^ Collectively , these data lead us to propose a possible autocrine mechanism involving VEGF operating by way of Flt 1 , in which ATL cells up regulate their own chemotaxis to facilitate their invasion into various organs . . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Vascular endothelial growth factor ( VEGF ) binds to and mediates its activity mainly through two tyrosine kinase receptors , VEGF receptor 1 [ or fms like tyrosine kinase receptor ( Flt 1 ) ] and VEGF receptor 2 [ or kinase insert domain containing receptor ( KDR ) ] . ^^^ Here we constructed a recombinant bifunctional diabody that is capable of blocking both Flt 1 and KDR from binding to their ligands , including VEGF and placenta growth factor ( PlGF ) . ^^^ The diabody retained the capacity to bind both KDR and Flt 1 and effectively blocked interaction between KDR and VEGF , Flt 1 and VEGF , and Flt 1 and PlGF . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
OBJECTIVE : To evaluate the expression of mRNA of vascular endothelial growth factor ( VEGF ) , its receptors Flt 1 and KDR / Flk 1 , and Ets 1 in human corpora lutea . ^^^ MAIN OUTCOME MEASURE ( S ) : Expression of VEGF , Flt 1 , KDR / Flk 1 , and Ets 1 in human corpora lutea on northern blot analysis or immunohistochemistry . ^^^ CONCLUSION ( S ) : Levels of mRNA of VEGF and its receptors Flt 1 and KDR / Flk 1 in human luteal cells may be related to luteal function . . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
METHODS : Concentrations of VEGF and its soluble receptor , soluble fms like tyrosine kinase 1 ( sFlt 1 ) , were measured by enzyme linked immunosorbent assay in serum samples from patients with early ( < 2 years from onset ) arthritic symptoms in the peripheral joints , namely early rheumatoid arthritis ( RA ) , self limiting arthritis ( viral , reactive , and idiopathic inflammatory arthritis ) , or psoriatic arthritis . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
The VEGF receptor , flt 1 , was associated with ganglion cells and the inner nuclear layer of normal and experimental mice and it was also demonstrated around areas of necrosis . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Altered placental and circulating levels of vascular endothelial growth factor ( VEGF ) and its receptor ( flt 1 ) may be associated with pre eclampsia and intrauterine growth restriction ( IUGR ) . ^^^ The aim of this study was to determine whether chorionic villous VEGF or flt 1 mRNA are altered at early gestation in pregnancies subsequently found to be complicated by abnormal fetal growth . ^^^ Quantitative reverse transcription polymerase chain reaction was performed on chorionic villous samples for VEGF and flt 1 using an internal RNA standard . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
In the present study , we analyzed the expression of VEGF and its receptors ( Flk 1 and Flt 1 ) in a rabbit model of collateral artery growth after femoral artery occlusion . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
In order to study basic factors related to angiogenesis and phenotypic changes of the capillaries located in tumor bearing alveolar walls , we examined 37 primary lung adenocarcinomas with electron microscopy and confocal laser scanning microscopy with antibodies for TM , vWf , vascular endothelial growth factor ( VEGF ) , and its receptors ( KDR and Flt 1 ) , and proliferating markers ( Ki 67 / proliferating cell nuclear antigen ) . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
The vascular endothelial growth factor ( VEGF ) receptor Flt 1 has been shown to be involved in vasculogenesis and angiogenesis . ^^^ The minimal extracellular domains of Flt 1 to achieve VEGF binding were screened using the yeast two hybrid system . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Increases in vascular endothelial growth factor ( VEGF ) receptors Flt 1 and Flk 1 / KDR , angiopoietin receptors Tie 1 , Tie 2 , and platelet derived growth factor receptor beta ( PDGF Rbeta ) , modest increases in epidermal growth factor receptor ( EGF R ) , and no increase in hepatocyte growth factor receptor ( c Met ) or fibroblast growth factor receptors ( FGF R ) were observed in isolated membranes during EC proliferation . ^^^ Although Flt 1 was phosphorylated on isolated hepatocytes , vascular endothelial growth factor ( 165 ) ( VEGF ( 165 ) ) did not induce a proliferative or motogenic response . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
The aim of this study was to investigate the expression of vascular endothelial growth factor ( VEGF ) receptors , the fms like tyrosine kinase ( flt 1 ) and kinase insert domain containing region ( KDR ) , in corpora lutea obtained at different stages of the oestrous cycle and during pregnancy in rats . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Vascular endothelial cell growth factor ( VEGF ) binds to 2 related receptor tyrosine kinases , known as kinase insert domain containing receptor ( KDR ) and fms like tyrosine kinase ( Flt 1 ) . ^^^ The Flt 1 receptor has been suggested to mediate VEGF stimulated endothelial branching morphogenesis , a process whereby endothelial cells , in the presence of a 3D milieu composed of extracellular matrix components and a mixture of growth factors , undergo a morphological transition into a tubular network with many lumina . ^^^ In the present study , we have used 2 independent endothelial cell tube formation models and highly selective VEGF mutants for the KDR and Flt 1 receptors . ^^^ In addition , we demonstrate a modulatory role for Flt 1 in VEGF mediated tube formation . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
METHODS : The expression and localization of the mRNA of VEGF and its receptors , flt 1 and flk 1 , were analyzed in the kidneys of puromycin aminonucleoside injected rats by use of Northern blotting and in situ hybridization . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
We studied expression of the genes encoding EPAS 1 , VEGF , VEGFR 1 , and 2 , Tie 2 , and its ligands , angiopoietin ( Ang ) 1 and 2 . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Blockade of in vivo VEGF mediated angiogenesis by antisense gene therapy : role of Flk 1 and Flt 1 receptors . ^^^ Vascular endothelial growth factor ( VEGF ) is a mitogenic and chimiotactic factor capable of inducing angiogenesis through the activation of its receptors , fetal liver kinase 1 ( Flk 1 ) and fms like tyrosine kinase 1 ( Flt 1 ) , expressed on endothelial cells . ^^^ The purpose of the present study was to assess if a treatment with antisense ( AS ) oligonucleotides directed against VEGF receptors Flk 1 or Flt 1 mRNA could prevent VEGF mediated angiogenesis . ^^^ With the use of miniosmotic pumps , phosphate buffered saline , VEGF , or VEGF combined with AS Flk 1 , AS Flt 1 , or AS scrambled oligonucleotides were released in mouse testis for 14 days . ^^^ The combination of AS Flk 1 or AS Flt 1 ( 200 microg ) to VEGF ( 2 . 5 microg ) reduced by 87 and 85 % the formation of new blood vessels , respectively , and the expression of their corresponding proteins . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Recent studies employing systemic or local administration of anti angiogenic drugs ( TNP 470 or fumagillin ) or specific VEGF antagonists ( VEGF antibody or soluble VEGFR 1 ) demonstrate a vital role for normal angiogenesis and VEGF action in follicle development , ovulation , or corpus luteum function . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Assessment of angiogenesis may yield important information for an effective antiangiogenic treatment for hepatocellular carcinoma ( HCC ) because HCC is characteristically hypervascular We examined the relationship of microvessel density ( MVD ) , vascular endothelial growth factor ( VEGF ) , and VEGF receptors Flt 1 and Flk 1 / KDR in 50 patients with HCC and in 3 hepatoma cell lines . ^^^ Tumor Flt 1 mRNA significantly correlated with tumor VEGF mRNA levels . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Here we demonstrate that both nonselective ( indomethacin ) and COX 2 selective ( NS 398 ) NSAIDs inhibit hypoxia induced in vitro angiogenesis in gastric microvascular endothelial cells via coordinated sequential events : 1 ) increased expression of the von Hippel Lindau ( VHL ) tumor suppressor , which targets proteins for ubiquitination leading to 2 ) reduced accumulation of hypoxia inducible factor 1alpha ( HIF 1alpha ) and , as a result , 3 ) reduced expression of vascular endothelial growth factor ( VEGF ) and its specific receptor Flt 1 . ^^^ Because HIF 1alpha is the major trigger for hypoxia induced activation of the VEGF and Flt 1 genes , this could explain how NSAIDs inhibit hypoxia induced angiogenesis . ^^^ Taken together , these results indicate that NSAIDs inhibit hypoxia induced angiogenesis in endothelial cells by inhibiting VEGF and Flt 1 expression through increased VHL expression and the resulting ubiquitination and degradation of HIF 1alpha . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
VEGF exerts its biologic activities through 2 transmembrane tyrosine kinase receptors : the fms like tyrosine kinase receptor ( Flt 1 , or VEGFR 1 ) and kinase insert domain containing receptor ( KDR or VEGFR 2 ) . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Although much is known about the biology of VEGF and its cognate receptors , VEGFR 1 and VEGFR 2 , the role of a recently identified co receptor for VEGF , neuropilin 1 , is not well understood . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
This paper studies the immunohistochemical expression of vascular endothelial growth factor ( VEGF ) and its receptors Flt 1 and Flk 1 , and basic fibroblast growth factor ( bFGF ) in BOOP and UIP . ^^^ The expression of Flt 1 and Flk 1 was in agreement with the expression of VEGF . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
CONCLUSIONS : ( 1 ) Vascular lesion of spleen is one part of portal hypertensive vascular lesions . ( 2 ) The mechanisms of these lesions are due to the changes of microcirculation and hemodynamics , immune disorders , increasing growth factors such as VEGF , Flt 1 and bFGF both at protein and transcription levels . . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Vascular endothelial growth factor ( VEGF ) mRNA becomes markedly up regulated by Nox 1 both in cultured cells and in tumors , and VEGF receptors ( VEGFR 1 and VEGFR 2 ) are highly induced in vascular cells in Nox 1 expressing tumors . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
The animals were euthanized at 1 , 2 , and 4 weeks after stenting , and western blot and immunohistochemical analysis were performed using VEGF , fms like tyrosine kinase ( flt ) 1 , and platelet derived growth factor ( PDGF ) antibodies . ^^^ The expressions of VEGF and flt 1 protein in the neointima were observed as early as 1 week after stenting and remained for up to 4 weeks , while re endothelialization was complete at 2 weeks . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Both cell clones expressed EC markers , which include factor 8 , VEGF receptors ( Flt 1 and KDR / Flk 1 ) , and CD 34 , and endocytosed acetylated low density lipoproteins . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
BACKGROUND : Recent studies show that vascular endothelial growth factor ( VEGF ) and its receptors Flt 1 and KDR , and a series of other angiogenic molecules , are upregulated in advanced but not low stage human neuroblastoma . ^^^ The mRNA expression of VEGF ( 165 ) and its receptors Flt 1 , KDR , NRP 1 , and NRP 2 was evaluated by real time reverse transcription polymerase chain reaction . ^^^ RESULTS : VEGF ( 165 ) mRNA was upregulated in Stage 3 and 4 and Flt 1 and KDR gene expression was increased in Stage 3 , while NRP 1 and 2 mRNA and protein levels were higher in Stages 1 4 vs . controls ( P < 0 . 05 ) . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Monoclonal antibodies to vascular endothelial growth factor ( VEGF ) and the VEGF receptor , FLT 1 , inhibit the growth of C 6 glioma in a mouse xenograft . ^^^ Monoclonal antibodies raised to peptide sequences of vascular endothelial growth factor ( VEGF ) and the VEGF receptor , FLT 1 , inhibited the growth of C 6 tumors growing subcutaneously in nude mice . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
TSA inhibited in a dose dependent and reversible fashion the VEGF induced expression of VEGF receptors , VEGFR 1 , VEGFR 2 , and neuropilin 1 . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Vascular endothelial growth factor ( VEGF ) is a peptide growth factor specific for the tyrosine kinase receptors VEGF receptor 1 and 2 ( VEGFR 1 and R 2 ) . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Angiogenic vascular endothelial growth factor ( VEGF ) , a likely mediator of these events , acts on the endothelium via a specific receptor , Flt 1 . ^^^ We hypothesized that patients with different manifestations of atherosclerosis , and others with diabetes , would have altered plasma levels of VEGF and Flt 1 compared with healthy individuals . ^^^ VEGF and soluble Flt 1 ( sFlt 1 ) were measured by ELISA . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
METHODS AND RESULTS : We investigated the expression of VEGF and its receptors ( flt 1 , KDR ) in acute KD tissues at 7 days to 5 weeks of illness . ^^^ Abundant expression of VEGF and flt 1 was documented immunohistochemically in many organs from acute KD , including heart and lung . ^^^ VEGF and flt 1 were colocalized in all vessels that showed edema . ^^^ Compared with controls , coronary vessels of acute KD had upregulation of VEGF and flt 1 but not KDR or neuropilin 1 . ^^^ CONCLUSIONS : These findings suggest that VEGF and flt 1 are upregulated in blood vessels in many organs of acute KD . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Vascular endothelial growth factor ( VEGF ) , transforming growth factor beta ( 1 ) ( TGF beta ( 1 ) ) , basic fibroblast growth factor ( bFGF ) , Flt 1 , and Flk 1 mRNA were analyzed from the left gastrocnemius by quantitative Northern blot . ^^^ Ethanol increased VEGF , TGF beta ( 1 ) , bFGF , and Flt 1 mRNA at rest and after acute exercise . ^^^ These findings suggest that 1 ) ethanol can increase skeletal muscle angiogenic growth factor gene expression and 2 ) the mechanisms responsible for the ethanol induced increases in VEGF , TGF beta ( 1 ) , and Flt 1 mRNA appear to be different from those responsible for exercise induced regulation . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
In this study , we investigated the expression of VEGF related factor genes ( VEGF , VEGF B , and VEGF C ) and their receptor genes ( VEGFR 1 and VEGFR 2 ) in renal cell carcinoma ( RCC ) . ^^^ There were significant differences in the expression level of VEGF , VEGFR 1 and VEGFR 2 between RCC and the corresponding normal renal tissue . ^^^ The expression level of VEGF in the tumor tissue significantly correlated with those of VEGFR 1 and VEGFR 2 . ^^^ A moderate to high protein expression for VEGF , VEGFR 1 , and VEGFR 2 was observed in both the tumor cells and the endothelial cells , whereas the protein expression was low for VEGF B and VEGF C . ^^^ The present results suggested that VEGF and its receptors VEGFR 1 and VEGFR 2 cooperates to play a crucial role in the angiogenesis of RCC , while VEGF B and VEGFR C may not . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
The flt 1 gene encodes for both the full length receptor Flt 1 ( VEGFR 1 ) and a soluble form designated sFlt 1 . sFlt 1 carries the VEGF binding domain of Flt 1 as well as a 31 amino acid stretch derived from an intron and tightly binds VEGF , suppressing its angiogenic activity . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Soluble vascular endothelial growth factor receptor 1 ( sVEGFR 1 ) , a naturally occurring soluble form of VEGFR 1 , is a negative counterpart of the vascular endothelial growth factor ( VEGF ) signaling pathway , which has been characterized as one of the most important endothelial regulators in human tumor angiogenesis . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Three classes of disulfide constrained peptides that antagonize binding of the VEGF dimer to its receptors , KDR and Flt 1 , were identified previously using phage display methods . ^^^ The v 107 binding site on VEGF overlaps partially with the binding site of KDR and is similar to that for domain 2 of Flt 1 . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Specifically , 1 ) VEGF / Flt and Ang 1 / Tie 2 may promote trophoblast growth , 2 ) VEGF / KDR and Ang 1 / Tie 2 may support fetoplacental vascular development and stabilization , 3 ) sFlt may balance VEGF actions , and 4 ) Ang 2 / Tie 2 may remodel the maternal vasculature . . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
We blocked VEGF activity in vivo by transfecting with plasmid DNA encoding the murine soluble FLT 1 ( sFLT 1 ) gene into thigh muscle . ^^^ Soluble FLT 1 can suppress VEGF activity both by sequestering VEGF and by functioning as a dominant negative inhibitor of VEGF receptors . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
The angiogenic growth factor vascular endothelial growth factor ( VEGF ) and its receptor fms like tyrosine kinase receptor ( Flt 1 ) were significantly decreased in CLD . ^^^ These data suggest that CLD impairs lung microvascular development and that a possible mechanism is disruption of VEGF and Flt 1 expression . . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
We have previously shown that 4 anilinoquinazolines can be potent inhibitors of vascular endothelial growth factor ( VEGF ) receptor ( Flt 1 and KDR ) tyrosine kinase activity . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Immunohistochemistry for vascular endothelial growth factor ( VEGF ) and its receptors , fms like tyrosine kinase ( flt 1 ) and kinase insert domain containing region ( KDR ) , was performed on human endometrium obtained from patients with normal menstrual cycles , patients given oestrogen and progesterone , and women in early pregnancy . ^^^ Endometrial stromal cells isolated from proliferative phase endometrium were incubated with oestrogen ( 10 ( 8 ) mol l 1 ) and medroxyprogesterone acetate ( MPA ; 10 ( 6 ) mol l 1 ) for 18 days to study the regulation of VEGF , flt 1 and KDR in endometrial stromal cells by oestrogen and progesterone . ^^^ Expression of VEGF and KDR mRNAs was increased significantly by oestrogen and MPA , accompanied by decidualization , whereas flt 1 mRNA expression was not affected . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Mice lacking the vascular endothelial growth factor ( VEGF ) receptor flt 1 die of vascular overgrowth , and we are interested in how flt 1 normally prevents this outcome . ^^^ Our results support a model whereby aberrant endothelial cell division is the cellular mechanism resulting in vascular overgrowth , and they suggest that VEGF dependent endothelial cell division is normally finely modulated by flt 1 to produce blood vessels . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Vascular endothelial growth factor ( VEGF ) acts primarily as an endothelial cell mitogen via the specific receptors Flk 1 and Flt 1 . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Blood retinal barrier breakdown is markedly increased with acute intensive insulin therapy but can be reversed by treating animals with a fusion protein containing a soluble form of the VEGF receptor Flt ; a control fusion protein has no such protective effect . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
The receptors , VEGFR 1 ( FLT 1 ) and VEGFR 2 ( KDR / Flk 1 ) , are upregulated on vascular endothelial cells in tumors by hypoxia and by the increased local concentration of VEGF . ^^^ A promising monoclonal antibody is 2C3 , which blocks VEGF from binding to VEGFR 2 but not VEGFR 1 . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
A significant inhibition of the angiogenic effect of PAF was achieved by anti VEGF antibodies or soluble VEGF receptors such as KDR and flt 1 but not by antibodies against tumor necrosis factor alpha , interleukin 1alpha , or basic fibroblast growth factor . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Although the production levels of vascular endothelial growth factor ( VEGF ) in pancreatic cancer cell lines are different , anti angiogenesis gene therapy using a soluble form of VEGF receptor ( flt 1 ) has been demonstrated to be a promising strategy for pancreatic cancer . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
To investigate the effects of stable expression of the soluble form of Flt 1 VEGF receptor ( sFlt 1 ) , a known endogenous inhibitor of VEGF , on the malignant ascites tumors , we cotransduced RMG 1 human ovarian cancer cells with adeno associated virus vectors carrying the sFlt 1 cDNA and Neo gene or Neo gene alone and isolated both the sFlt 1 expressing clone and the Neo expressing clone . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
In the course of a 24 hr in vitro maturation procedure ( IVM ) , expression of the smaller VEGF transcripts and their specific receptors flt and flk changed remarkably in a time dependent manner as observed by RT PCR . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Immunohistochemical results and semi quantitative reverse transcriptase polymerase chain reaction indicated that the VEGF receptors flk 1 and flt 1 were up regulated in response to the infusion trauma ; flt 1 was localized to reactive astroglia , while flk 1 was expressed in vascular endothelium but predominantly in neuronal somata and processes adjacent to the delivery site . mRNA for the VEGF ( 121 ) , VEGF ( 165 ) and VEGF ( 188 ) isoforms was also increased after delivery of the recombinant protein . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
We investigated the expression pattern of NP 1 / NP 2 , their co receptors , vascular endothelial growth factor receptor 1 and 2 ( VEGFR 1 , VEGFR 2 ) , and their ligands , class 3 semaphorins , VEGF and PLGF , following experimental cerebral ischemia in mice . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Both VEGF and PlGF , acting through the tyrosine kinase receptors VEGFR 1 and VEGFR 2 , have been implicated in playing a role in ovine placental vascular development . ^^^ Furthermore , alterations in VEGF , VEGFR 1 and VEGFR 2 mRNA expression , during the period of maximal placental growth , may contribute to the development of placental insufficiency , and ultimately intrauterine growth restriction . . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
The levels of vascular endothelial growth factor ( VEGF ) , its receptors VEGFR 1 and VEGFR 2 , and angiopoietin 1 mRNA tended to be higher in the mammary fat pad tumors than in the cranial tumors ( 1 . 5 , 1 . 5 , 3 , and 2 fold , respectively ) . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Tumors removed at 1 week intervals up to week 6 were probed by immunohistochemistry ( n = 3 11 samples ) for vascular endothelial growth factor ( VEGF ) , placental growth factor ( PlGF ) , flk 1 and flt 1 , angiopoietin 1 and 2 , and Tie 1 and 2 . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Immunohistochemistry on paraffin sections was performed with anti human factor 8 antibody to study the microvascular density ( MVD ) , and with antibodies to VEGF , Flt 1 , and KDR to investigate the expression of these three proteins in different cellular compartments . ^^^ Survival analysis showed that the high MVD group was associated with a high risk of death . ( 3 ) There was no significant difference between low MVD and high MVD groups in VEGF expression in tumor cells , but in endothelial cells both Flt 1 and KDR were correlated with high microvessel count . ( 4 ) The high expression of VEGF in tumor cells was correlated with that in stromal fibroblasts . ^^^ The level of expression in both cells was consistent . ( 5 ) In both tumor cells and endothelial cells , high co expression of VEGF and KDR was consistent , but that of VEGF and Flt 1 showed inconsistency . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
It was hypothesized that , upon binding to VEGF , Flt 1 tyrosine kinase underwent dimerization and initiated the signal transduction in VEGF / VEGF receptor system . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Renal ischemia reperfusion increases endothelial VEGFR 2 without increasing VEGF or VEGFR 1 expression . ^^^ Expression of the angiogenic growth factor vascular endothelial growth factor ( VEGF ) and its receptors ( VEGFR 1 and VEGFR 2 ) is up regulated by hypoxia in a variety of organs and cell lines . ^^^ RESULTS : VEGFR 2 mRNA and protein expression were up regulated without an increase in VEGF or VEGFR 1 expression . ^^^ CONCLUSION : VEGF and VEGFR 1 expression are not increased by renal ischemia and ischemia reperfusion injury . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Using quantitative RT PCR , VEGF and its receptors , Flk 1 and Flt 1 , were detected in CK MVECs . ^^^ CK+ MVECs expressed VEGF and Flt 1 mRNA , but were devoid of Flk 1 transcripts . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Small molecules that selectively inhibit the VEGF receptor associated tyrosine kinase activities of Flk 1 ( KDR ) and Flt 1 have been developed . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Vascular Endothelial Growth Factor ( VEGF ) through interaction with its receptors VEGFR 2 and VEGFR 1 expressed on endothelial and hematopoietic stem cells promote recruitment of these cells into the sites of tissue injury accelerating vascular healing . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Vascular endothelial growth factor ( VEGF ) , an important angiogenic factor , regulates cell proliferation , differentiation , and apoptosis through activation of its tyrosine kinase receptors , such as Flt 1 and Flk 1 / Kdr . ^^^ Inactivation of the VEGF signal transduction pathway by expression of soluble form of Flt 1 inhibited Flk 1 / Kdr activation and HMC proliferation induced by SV 40 early genes . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
To explore the therapeutic potential of blocking vascular endothelial growth factor ( VEGF ) and angiopoietin ( Ang ) pathways in IBC , established vectors encoding soluble Flt 1 ( sFlt 1 ) and soluble Tie 2 ( sTie 2 ) were injected directly into WIBC 9 . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
OCs and pOCs in the arthritic joints express VEGF and VEGF receptor type 1 ( Flt 1 ) . ^^^ Raw cells also express Flt 1 , and VEGF treatment stimulated chemotaxis , cell proliferation , the association of Flt 1 with focal adhesion kinase ( FAK ) , and the tyrosine phosphorylation of FAK in Raw cells . ^^^ Our results suggest the possible involvement of the VEGF Flt 1 FAK pathway in inflammatory disease induced joint destruction . . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Many cellular effects of thrombin on endothelial cells can contribute to the angiogenic action of thrombin . ( 1 ) Exposure of endothelial cells to thrombin cause a time and dose dependent decrease in the attachment of these cells to basement membrane components , with a concomitant increase in matrix metalloproteinase 2 activation . ( 2 ) Thrombin upregulates the expression of integrin alphavbeta 3 , the marker of the angiogenic phenotype of endothelial cells . ( 3 ) Thrombin has chemotactic and aptotactic effects on endothelial cells and upregulates the expression of the vascular endothelial growth factor ( VEGF ) receptors ( KDR and Flt 1 ) . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Among the known stem cell active chemokines , the angiogenic factor VEGF through interaction with its receptors , VEGFR 2 and VEGFR 1 expressed on endothelial and hematopoietic stem cells , promotes mobilization and recruitment of these cells into the neo angiogenic sites , thereby accelerating the revascularization process . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
In cloning tyrosine kinase genes in dog prostate cells , a fragment of the vascular endothelial growth factor ( VEGF ) receptor 1 or Flt 1 was sequenced . ^^^ That the major Flt 1 tyrosine phosphorylated protein was likely VEGF R 1 and part of VEGF signaling pathways was shown by enhanced level of only this protein when PC 3 cells were exposed to VEGF . ^^^ Accordingly specific cell surface receptor complexes , displaced by VEGF but not EGF and compatible with Flt 1 in size , were revealed by chemical cross linking after 125I VEGF binding . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Using a quantitative solid phase radioimmunoassay ( RIA ) , we measured the cellular protein levels of Bcl 2 , cyclin D 1 , PCNA , ATM , Fas , Bax , retinoic acid receptor alpha ( RARalpha ) , retinoic acid receptor beta ( RXRbeta ) , Flt 1 , VEGF , and cellular beta 2 microglobulin in 230 samples of CLL . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Here we demonstrate the presence of the related VEGF receptors fms like tyrosine kinase 1 and kinase insert domain region on human platelets . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
VEGF has 2 receptor tyrosine kinases , KDR and Flt 1 . ^^^ The KDR selective mutant induced vascular endothelial cell proliferation comparable to VEGF , whereas the Flt 1 selective mutant had no effect on proliferation . ^^^ Similarly , VEGF and KDR selective mutant induced more potent vasorelaxation than Flt selective mutant or placenta growth factor that binds Flt 1 only ( P < 0 . 01 ) , and the vasorelaxation to KDR selective mutant was not significantly different at low concentrations but less than that to VEGF at high concentrations . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
To inhibit vascular endothelial growth factor ( VEGF ) , a soluble combined truncated form of the fms like tyrosine kinase ( Flt ) and kinase insert domain containing receptor ( KDR ) receptor fused to IgG ( VEGF Trap R1R2 ) was administered for 10 d during the follicular phase of the cycle . ^^^ Effects on gene expression of VEGF and its receptors , Flt and KDR , were studied by in situ hybridization . ^^^ VEGF expression in granulosa and thecal cells increased after treatment , whereas Flt and KDR expressions in thecal endothelial cells were markedly decreased . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Gene expression of adhesion molecules , interleukin 1alpha ( IL 1alpha ) , IL 1beta , monocyte chemoattractant protein 1 , macrophage inflammatory protein 1alpha ( MIP 1alpha ) , MIP 2 , transforming growth factor beta 1 ( TGF beta 1 ) connective tissue growth factor ( CTGF ) , vascular endothelial growth factor ( VEGF ) , Flt 1 , and Flk 1 was enhanced at the wound site . ^^^ In KO mice , an enhancement in angiogenesis , collagen content , and reepithelialization was accelerated with the increased gene expression of TGF beta 1 , CTGF , VEGF , Flt 1 , and Flk 1 at the wound sites , resulting in accelerated wound healing compared with WT mice . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
However , blocking VEGF by administering a soluble VEGFR 1 , which acts extracellularly , induced only minor effects . ^^^ Not only ligands selective for VEGF and VEGFR 2 but also VEGFR 1 agonists rescued survival and repopulation of VEGF deficient HSCs , revealing a function for VEGFR 1 signalling during haematopoiesis . . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
As VEGF exerts its effects via two receptors , VEGF receptor 1 ( VEGFR 1 ) and VEGFR 2 , we evaluated the significance of VEGFR 1 and VEGFR 2 protein levels in AML and myelodysplastic syndrome ( MDS ) , and their relationship to VEGF protein levels . ^^^ We found a significant correlation between VEGF and VEGFR 2 levels in both AML and MDS ( P < 0 . 0000001 andP < 0 . 0002 respectively ) but not between VEGF and VEGFR 1 levels . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
We investigated the vascular endothelial growth factor ( VEGF ) receptor [ fms like tyrosine kinase ( Flt 1 and fetal liver kinase 1 ( Flk 1 ) ] response to acute exercise . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
In both models , expression of VEGF correlated with expression of mRNAs encoding other angiogenic cytokines ( angiopoietin 1 and angiopoietin 2 ) , endothelial cell receptor tyrosine kinases ( Flt 1 , KDR , Tie 1 ) , and endothelial cell adhesion molecules ( VE cadherin , PECAM 1 ) . ^^^ Relative to control , in dermis highly stimulated by VEGF , the Ang 2 mRNA transcript numbers increased 35 fold , PECAM 1 and VE cadherin increased 10 fold , Tie 1 increased 8 fold , KDR and Flt 1 each increased 4 fold , and Ang 1 increased 2 fold . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
VEGF exerts its effect via the tyrosine kinase receptors Flt 1 and KDR / Flk 1 . ^^^ Physiologic and histologic changes were studied in addition to the mRNA expression of VEGF and its receptors Flt 1 and KDR / Flk 1 by Northern blot , and the protein expression of VEGF by Western blot . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
We performed immunohistochemical analysis using specific antibodies to VEGF and to the known VEGF receptors , VEGFR 1 and VEGFR 2 , on paraffin sections of human nasal polyps ( n=11 ) and controls of human mucosa of the normal middle turbinates ( n=6 ) . ^^^ These findings support a role for VEGF and its receptors , VEGFR 1 and VEGFR 2 , in the development and perpetuation of edema and angiogenesis in nasal polyps . . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
The aim of the present study was to evaluate the expression of VEGF and of its two high affinity tyrosine kinase receptors ( KDR and Flt 1 ) in neuroblastoma surgical samples and cell lines . ^^^ PROCEDURE : The VEGF , KDR , and Flt 1 mRNA expression in neuroblastoma surgical samples and cell lines was studied by RT PCR . ^^^ It was also possible to demonstrate that the SK N BE cell line expressed VEGF , KDR , and Flt 1 mRNAs as well as biologically active receptors : The cells bound [ ( 125 ) 1 ] VEGF , and their growth was stimulated by exogenous VEGF . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
VEGF induced capillary like structure formation was inhibited by sequestration of VEGF via addition of soluble Flt 1 fusion protein or anti VEGF antibodies . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Human lactiferous mammary gland cells produce vascular endothelial growth factor ( VEGF ) and express the VEGF receptors , Flt 1 AND KDR / Flk 1 . ^^^ MEC was also expressed VEGF receptors , flt 1 and Flk 1 / KDR . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
OBJECTIVE : To investigate the expression of vascular endothelial cell growth factor ( VEGF ) and its receptor ( Flt 1 ) in breast carcinoma and the relationship of such expression with angiogenesis , tumor invasion and metastasis . ^^^ METHODS : In situ hybridization and immunohistochemistry technique were used to test the expression of mRNA and protein expression of VEGF and flt 1 in 48 specimens of breast carcinoma . 48 specimens of tissues near cancer , 3 specimens of fibroadenoma of breast , and 3 specimens of normal breast were used as controls . ^^^ The microvascular density ( MVD ) was significantly greater in VEGF and Flt 1 higher expression groups than in lower groups ( P < 0 . 01 ) . ^^^ Both VEGF expression and Flt 1 expression were well correlated with the histological grade and lymph node metastasis . ^^^ Test of the expression of VEGF and Flt 1 may act as a reliable index to determine angiogenesis , malignancy , invasion and metastasis . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Although SU 5614 was a potent inhibitor of the VEGF induced endothelial cell sprouting in vitro , the sensitivity of leukemic cells toward the growth inhibitory activity of the compound was determined by the c kit , but not by the VEGFR 1 / 2 expression . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Since factors regulating angiogenesis in tendons are largely unknown , we analyzed the expression of the vascular endothelial growth factor ( VEGF ) and its receptors VEGFR 1 ( flt 1 ) and VEGFR 2 ( KDR ) in human fetal and adult tendon tissue by immunohistochemical , biochemical , and molecular biology methods . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
The most abundant isoform , VEGF 165 , binds to vascular endothelial growth factor receptors 1 ( Flt 1 ) and 2 ( KDR / Flk 1 ) with picomolar affinity . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
VEGF acts via two high affinity receptors : KDR and FLT 1 . ^^^ There was no significant correlation between serum VEGF and s FLT 1 . ^^^ CONCLUSION : We conclude that s FLT 1 , a natural antagonist of VEGF , is detectable in the sera of cancer patients . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
At different stages of tumor growth , the histological aspects were described and sections were immunostained for VEGF , Ang 2 and their receptors VEGFR 1 , VEGFR 2 and Tie 2 . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Three anti VEGF agents were tested : an anti human VEGF ( 165 ) RNA based fluoropyrimidine aptamer ; a monoclonal anti human VEGF antibody ; and VEGF Trap , a composite decoy receptor based on VEGFR 1 and VEGFR 2 fused to an Fc segment of IgG 1 . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
During early fetal angiogenesis , vascular endothelial growth factor ( VEGF ) and its receptors kinase insert domain containing receptor ( KDR ) and fms like tyrosine kinase 1 ( Flt 1 ) are required for the development of the systemic vasculature . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Targeted disruption of vascular endothelial growth factor ( VEGF ) or its receptor kdr ( flk 1 , VEGFR 2 ) in mouse embryos results in a severe reduction of all blood vessels , while the complete loss of flt 1 ( VEGFR 1 ) leads to an increased number of hemangioblasts and a disorganized vasculature . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Here , developmental changes in mRNA expression for four splicing variants of vascular endothelial growth factor ( VEGF ) and for two distinct VEGF receptors ( Fms like tyrosine kinase ( Flt 1 ) and kinase insert domain containing receptor / fetal liver kinase 1 ( KDR / Flk 1 ) ) were studied in rat masseter . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Our prior studies show that multiple myeloma ( MM ) cell lines and patient cells express high affinity vascular endothelial growth factor ( VEGF ) receptor ( VEGFR ) Flt 1 but not Flk 1 / KDR . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
AMF exposure stimulated the Flt 1 expression on human umbilical vein endothelial cells ( HUVECs ) surface and this AMF treated cells exhibited high responsibility against VEGF . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
The VEGF receptors fetal liver kinase 1 ( Flk 1 ) and Fms like tyrosine kinase 1 ( Flt 1 ) become highly expressed in epithelial tumor and endothelial cells in the mammary carcinomas , suggesting a potential autocrine effect for VEGF on tumor cell growth . ^^^ However , mRNA levels of a variety of proangiogenic factors ( VEGF , VEGF receptors Flk 1 and Flt 1 , angiopoietin 2 , Tie 1 , cadherin 5 and PECAM ) were significantly decreased in the endostatin treated group compared with controls . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Expression of mRNA for the different VEGF splice forms and for VEGF receptors KDR and FLT 1 was analysed by reverse transcription polymerase chain reaction ( RT PCR ) . ^^^ The results of our study suggest that the splice forms VEGF 121 and VEGF 165 and the receptors KDR and FLT 1 of the known angiogenetic peptide VEGF play a role in process of endochondral ossification . . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
OBJECTIVE : To document the pattern of expression of the two angiogenic growth factors , vascular endothelial growth factor ( VEGF ) B and VEGF C , and of their receptors , VEGFR 1 , VEGFR 2 , and VEGFR 3 , in order to investigate their possible role in the regulation of angiogenesis in normal cycling human endometrium . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
We investigated the interaction between angiogenic and osteogenic factors in bone formation and bone healing with ex vivo gene therapy using muscle derived stem cells genetically engineered to express human bone morphogenetic protein 4 ( BMP 4 ) , VEGF , or VEGF specific antagonist ( soluble Flt 1 ) . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Vascular endothelial growth factor ( VEGF ) and its receptors , kinase insert domain containing region ( KDR ) and fms like tyrosine kinase ( Flt ) mRNA , were localized and quantified in in situ hybridization . ^^^ No major differences were found in expression of VEGF , Flt , or KDR in these CL . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
A semiquantitative evaluation of the labeling revealed an induction of genes encoding EPAS 1 , VEGF , VEGFR 1 , VEGFR 2 , endothelin receptor , type B ( ETB ) and endothelin receptor , type A ( ETA ) in malignant pheochromocytomas as compared to benign tumors . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
LtV NLS / LacZ transduced HUVECs showed gene expression of endothelial markers including CD 34 and flt 1 and KDR / flk 1 of vascular endothelial growth factor ( VEGF ) receptors and had angiogenic potential as efficiently as primarily cultured HUVECs in a Matrigel assay . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Vascular endothelial growth factor ( VEGF ) is a key regulator for placental angiogenesis and vascular functions via activating two high affinity tyrosine kinase receptors , VEGF receptor 1 ( VEGFR 1 ) and 2 ( VEGFR 2 ) . ^^^ Using PCR analysis , we also identified partial sequences of multiple VEGF isoforms ( VEGF 188 , 183 , 164 , and 120 ) as well as VEGFR 1 , VEGFR 2 , and neuropilin 2 ( NP 2 ) from the OFPAE cell cDNA library . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Autocrine paracrine VEGF loops potentiate the maturation of megakaryocytic precursors through Flt 1 receptor . ^^^ The functional role of VEGF release on Mk maturation is indicated by 2 series of observations . ( 1 ) Molecules preventing the VEGF Flt 1 interaction on the precursor membrane ( eg , soluble Flt 1 receptors ) significantly inhibit Mk polyploidization . ( 2 ) Addition of exogenous VEGF or placenta growth factor ( PlGF ) markedly potentiates Mk maturation . ^^^ Altogether , our results suggest that in the hematopoietic microenvironment an autocrine VEGF loop contributes to optimal Mk maturation through Flt 1 . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
A chemically defined culture of VEGFR2+ cells derived from embryonic stem cells reveals the role of VEGFR 1 in tuning the threshold for VEGF in developing endothelial cells . ^^^ Delayed expression of VEGFR 1 correlated with increased dose dependency of VEGF . ^^^ These results suggested that greater requirement of VEGF in the maintenance than induction of ECs was due to the activity of VEGFR 1 sequestering VEGF from VEGFR 2 signal . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
OBJECTIVE : This study evaluated the relative roles of the vascular endothelial growth factor ( VEGF ) receptors KDR and Flt 1 in the mediation of altered gene expression elicited by VEGF . ^^^ METHODS AND RESULTS : We used mutants of VEGF selective for the KDR and Flt 1 receptors to differentiate gene expression patterns mediated by wild type VEGF ( VEGFwt ) in human umbilical vein endothelial cells . ^^^ Furthermore , in human umbilical vein endothelial cells , the Flt 1 receptor appears to act as a decoy receptor , tempering the response to lower concentrations of VEGF . . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Western blot analysis also indicated that there is no difference in the levels of proteins which are directly involved in angiogenesis , such as VEGF , Flt 1 , and Flk 1 , between cells transfected with vector , p33ING1 , and antisense p33ING1 . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
AIM : The expression of vascular endothelial growth factor ( VEGF ) and its receptors KDR and Flt 1 by gastric carcinoma tissues and different gastric carcinoma cell lines was detected to elucidate the molecular mechanism of this growth factor in promoting tumor growth . ^^^ METHODS : The expression of VEGF , Flt 1 and KDR was determined by reverse transcription polymerase chain reaction ( RT PCR ) in gastric cancer cell lines RF 1 , RF 48 , AGS 1 , NCI N 87 , NCI SNU 1 , NCI SNU 5 , NCI SNU 16 and KATO 3 . ^^^ RESULTS : All 8 gastric cancer cell lines analyzed expressed VEGF ( 121 ) and VEGF ( 165 ) and six of them expressed both Flt 1 and KDR , while cell line NCI SNU 5 expressed Flt 1 only and cell line KATOIII expressed neither Flt 1 nor KDR . ^^^ CONCLUSION : VEGF and its receptors KDR and Flt 1 were expressed widely in gastric carcinoma cells and the VEGF stimulated KDR positive tumor cell growth directly . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Granulosa cells and thecal tissues in small ( diameter , < 4 mm ) , medium ( diameter , 4 5 mm ) , or large ( diameter , > 5 mm ) individual follicles were collected for detection of mRNA expression of vascular endothelial growth factor ( VEGF ) 120 , VEGF 164 , basic fibroblast growth factor ( bFGF ) , and epidermal growth factor ( EGF ) in granulosa cells and fms like tyrosine kinase ( Flt 1 ) , fetal liver kinase ( Flk 1 ) or the murine homologue of kinase domain region ( KDR ) , bFGF receptor ( bFGF R ) , and EGF receptor ( EGF R ) in thecal tissue by semiquantitative reverse transcription polymerase chain reaction . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Li cells was observed after infection with Ad antisense VEGF ; Effect of recombinant soluble VEGF receptor protein ( 3 ' delta Flt 1 ) on proliferation of ( human umbilical vascular endothelial cell ) HUVEC induced by VEGF was investigated ; Effect of both antisense VEGF and sflt 1 on neovascularization were tested after Ad anti VEGF or Ad sflt 1 injected in chorion of chicken embryo . ^^^ Li by recombinant Ad antisense VEGF is only 15 % of that from control group ( P < 0 . 01 ) ; the proliferation of HUVEC induced by VEGF in condition medium containing 3 ' delta Flt 1 is significantly reduced , and negatively related with dose within certain range ; Both sflt 1 and antisense VEGF nucleotide obviously inhibited formation of new blood vessel , even caused dead embryo . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
The vascular endothelial growth factor ( VEGF ) receptor family in mammals contains three members , VEGFR 1 ( Flt 1 ) , VEGFR 2 ( KDR / Flk 1 ) and VEGFR 3 ( Flt 4 ) , which directly regulate the formation of blood vessels and lymphatic vessels . ^^^ Recently , some novel findings on the phylogenetical conservation of VEGF receptor genes in animals were reported : the conservation of the VEGFR1 / soluble VEGFR 1 gene in birds , and the conservation of the VEGFR PDGFR like receptor gene in nonvertebrates . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
We have investigated the expression patterns of VEGF , placental growth factor ( PlGF ) , and their receptors fms like tyrosine kinase ( Flt 1 ) and kinase insert domain containing region ( KDR ) in placentas with histopathological changes . ^^^ Positive immunostaining for VEGF , PlGF , and Flt 1 was observed in infiltrated neutrophils and macrophages in the placentas with chorioamnionitis ( CAM ) . ^^^ These findings suggested that in the hypoxic / ischemic regions , VEGF and KDR expression is increased within the villous vessels by paracrine regulation , whereas the expression of PlGF and Flt 1 is enhanced in villous trophoblasts by autocrine regulation . ^^^ Furthermore , the results indicated that VEGF and PlGF stimulate inflammatory cell migration by autocrine regulation via the Flt 1 receptor in the CAM placenta . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
The data were insufficient to determine the prognostic value of VEGF in SCLC and that of its two receptors Flt 1 and KDR , with 1 , 2 and 1 published studies respectively . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
OBJECTIVE : To study the expression of vascular endothelial growth factor ( VEGF ) and its receptors , the fms like tyrosine 1 ( flt 1 ) and kinase insert domain containing receptor ( KDR ) in endometrial carcinoma and investigate the functions of VEGF and its receptors for endometrial carcinoma angiogenesis and its relation to the grade of tumor . ^^^ METHODS : Immunocytochemistry and in situ hybridization technique were used to measure the level of VEGF , flt 1 , KDR protein and mRNA in endometrial carcinoma tissue from 23 patients and endometrial samples from 6 normal menopausal women . ^^^ CONCLUSIONS : The expression pattern of VEGF , flt 1 and KDR protein and mRNA increased with the increase of tumor grade in endometrial carcinoma indicates that VEGF and its receptors contribute to the neovascularization of tumors and is one of the factors that relate to rapid tumor growth of endometrial carcinoma . . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
In the present study , we demonstrate the expression of mRNA for all four VEGF isoforms ( mVEGF ( 120 , 144 , 164 , 188 ) ) and for Flk 1 / KDR and Flt 1 receptors in the mouse adrenal in vivo . ^^^ Suppression of the pituitary adrenocortical axis by dexamethasone ( 0 . 5 mg 10 100 g body wt ( 1 ) 10 day ( 1 ) during 6 days ) induced a decrease in corticosterone levels , adrenal weights by 50 % ( P < 0 . 001 ) , VEGF ( 188 ) mRNA , and Flk 1 / KDR mRNA , whereas Flt 1 remained consistent during steroid treatment . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Interaction between vascular endothelial growth factor ( VEGF ) and its cognate receptors , KDR / Flk 1 and Flt 1 , of vascular endothelial cells is expected to induce an angiogenesis `` switch ' ' in tumors and other angiogenesis associated diseases . ^^^ In this study , we first observed that SU 5416 inhibited Flt 1 tyrosine kinase activity at similar doses , in addition to inhibiting KDR / Flk 1 tyrosine kinase activity in response to VEGF . ^^^ SU 5416 inhibited cell migration of human vascular endothelial cells expressing both Flt 1 and KDR in response to VEGF and also inhibited the cell migration in response to placenta growth factor ( PIGF ) , a specific ligand for Flt 1 . ^^^ The antiangiogenic effects by this VEGF receptor targeting compound appeared to be mediated through interference not only with KDR / Flk 1 but also with Flt 1 . ^^^ Cell migration of vascular endothelial cells and monocytic cells through Flt 1 , thus , might play a key role in VEGF induced tumor angiogenesis in concert with KDR / Flk 1 . . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Here we report that the human natural killer cell lines , NK 92 and YT , express the mRNA message and protein product for VEGF B and its receptor , VEGFR 1 / Flt 1 . ^^^ While stimulation of these cells by the potent angiogenic factor VEGF A 165 , which also binds to VEGFR 1 , does not alter the proliferation of the cells , it does increase adhesion to a model basement membrane like extracellular matrix , Matrigel . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
We report that vascular endothelial growth factor ( VEGF ) , a major angiogenic factor , is also arequisite autocrine factor for breast carcinoma invasion in vitro and that the VEGF receptor Neuropilin 1 but not Flt 1 is essential for this function . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
The biological significance of VEGF , EGF , and bFGF is mediated by their receptors , which belong to the family of tyrosine kinase receptors : Flt 1 ( VEGFR 1 ) , KDR ( VEGFR 2 ) , EGFR , FGFR 1 , FGFR 2 , FGFR 3 , and FGFR 4 . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Immunohistochemical detection of the vascular endothelial growth factor ( VEGF ) receptor Flt 1 ( VEGFR 1 ) was performed in paraffin embedded sections . ^^^ Finally , systemic adenoviral expression of a soluble Flt 1 / Fc construct was used to study the effect of inhibition of VEGF bioactivity on the appearance of goblet cells and neovascularization . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
The present study was conducted to examine changes of mRNAs encoding vascular endothelial growth factor ( VEGF ) and its receptors ( KDR / Flk 1 and Flt 1 ) , and CD 34 , which is known to be a specific marker for endothelial cells , during the development and maintenance of the caprine corpora lutea ( CL ) . ^^^ These results suggest that rise of KDR / Flk 1 and VEGF ( 165 ) mRNAs during the caprine CL development may be associated with enhanced angiogenesis and that increment of VEGF ( 165 ) and Flt 1 mRNAs during the CL maintenance may play nonangiogenic roles . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
RESULTS : In this report , we demonstrate that Src preferentially associates with KDR / Flk 1 rather than Flt 1 in human umbilical vein endothelial cells ( HUVECs ) , and that VEGF stimulation resulted in an increase of Src activity associated with activated KDR / Flk 1 . ^^^ CONCLUSIONS : Thus , Src preferentially associates with KDR / Flk 1 , rather than with Flt 1 , upon VEGF stimulation in endothelial cells . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
CONCLUSION : The peptide F 56 is an effective antagonist of VEGF binding to Flt 1 and has a potent utility in antiangiogenesis and inhibiting tumor growth . . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
We evaluated the ex vivo expression of IGF 1 , VEGF , and their receptors ( IGF IR , Flt 1 , and KDR ) in human penile cavernosal smooth muscle cells ( HCSMCs ) to identify cellular and molecular pathways involved in the regulation of penile tissue vascularity . ^^^ To evaluate gene expression of VEGF , Flt 1 , and KDR , total RNA was extracted from cavernosal cells and subjected to reverse transcriptase polymerase chain reaction ( RT PCR ) using custom synthesized primers . ^^^ RT PCR evaluation revealed the expression of four splice variants of VEGF messenger RNA ( VEGFs 121 , 145 , 165 , and 189 ) and two of its receptors ( Flt 1 and KDR ) . ^^^ VEGF 165 and VEGF 121 were the most abundant forms of messenger RNA and Flt 1 appeared to be the most prominent receptor type in these cells . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
This study was designed to explore the gene coexpression pattern of VEGF and its receptors ( Flt 1 and KDR ) in variety of human malignant cell lines . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Aplidine , a new anticancer agent of marine origin , inhibits vascular endothelial growth factor ( VEGF ) secretion and blocks VEGF VEGFR 1 ( flt 1 ) autocrine loop in human leukemia cells MOLT 4 . ^^^ This cell line is characterized not only by the ability to secrete VEGF , but also for the presence on its surface of the VEGF receptor 1 ( VEGFR 1 ) . ^^^ Here , we report the ability of aplidine to block the VEGF / VEGFR 1 loop . ^^^ We found that aplidine blocked VEGF secretion that was temporally followed by a decrease in both VEGF and VEGFR 1 production . ^^^ These data demonstrate that aplidine inhibits the growth and induces apoptosis in MOLT 4 cells through the inhibition of VEGF secretion which blocks the VEGF / VEGFR 1 autocrine loop necessary for the growth of these cells . . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Training produced initial three to sixfold increases in VEGF , VEGF receptors ( KDR and Flt ) , the angiopoietin receptor ( Tie 2 ) , and endothelial nitric oxide synthase mRNA , which dissipated before the increase in capillarity , and a substantial ( 30 to 50 fold ) but transient upregulation of monocyte chemoattractant protein 1 mRNA . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Efforts to therapeutically stimulate or inhibit vessel growth have been primarily focused on vascular endothelial growth factor ( VEGF ) and its receptor VEGFR 2 ( Flk 1 ) , while little attention has been devoted to the therapeutic potential for angiogenic disorders of placental growth factor ( PlGF ) , a VEGF family member , and its receptor VEGFR 1 ( Flt 1 ) . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
VEGF is a secreted growth factor that mediates its biological effects by binding to two transmembrane tyrosine kinase receptors , VEGFR 1 and VEGFR 2 . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
To understand the molecular basis of the biological activity of VEGF E , we have functionally mapped residues important for interaction of VEGF E with VEGFR 2 by exchanging the domains between VEGF E ( NZ 7 ) and PlGF , which binds only to VEGFR 1 ( Flt 1 ) . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
BACKGROUND : To clarify the clinical significance of vascular endothelial growth factor ( VEGF ) and its receptors , VEGFR 1 ( Flt 1 ) and VEGFR 2 ( Flk 1 ) , in esophageal cancers , we evaluated the relationships between the expression of VEGF and its receptors , tumor microvessel density ( MVD ) , clinicopathological factors and prognosis . ^^^ Although VEGF expression correlated significantly with the MVD ( p < 0 . 05 ) , the correlations between VEGFR 1 and VEGFR 2 expression and the MVD were not significant . ^^^ There were , however , significant intercorrelations in expression between VEGF , VEGFR 1 and VEGFR 2 . ^^^ However , although VEGF expression correlates significantly with coexpression of its receptors , VEGFR 1 and VEGFR 2 , these receptors do not appear to contribute directly to tumor progression . ^^^ Expression of vascular endothelial growth factor ( VEGF ) and its receptors ( Flt 1 and Flk 1 ) in esophageal squamous cell carcinoma . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Cloning and expression of VEGF receptor Flt 1 gene in S . lividans TK 24 ] . ^^^ Using RNA extracted from human umbilical vein endothelium cell as a template , the gene VEGF receptor Flt 1 was amplified by RT PCR . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Expression and prognostic relevance of vascular endothelial growth factor ( VEGF ) and its receptor ( FLT 1 ) in nephroblastoma . ^^^ AIMS : To investigate the prognostic relevance of vascular endothelial growth factor ( VEGF ) and its receptor Flt 1 in nephroblastoma and whether tumour microvessel density ( MVD ) immunoreactivity , determined by the CD 31 antigen , is related to the expression of VEGF and Flt 1 . ^^^ METHODS : The expression of VEGF and Flt 1 and MVD were investigated by means of immunohistochemical analysis in 62 Wilms ' s tumours . ^^^ RESULTS : In general , VEGF and Flt 1 were expressed in normal kidney parenchyma and to a variable extent in the three main components of Wilms ' s tumour , namely : the blastemal , epithelial , and stromal cells . ^^^ In tumour tissue , 52 % and 47 % of blastemal cells were positive for VEGF and Flt 1 , respectively . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Selective activation of VEGF receptor 1 ( VEGFR 1 ) stimulated hepatocyte but not endothelial proliferation in vivo and reduced liver damage in mice exposed to a hepatotoxin . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
After removing the urinary bladder of each rat , expression of VEGF and VEGF receptor FLT 1 , and new capillaries were examined by immunohistochemical staining . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Lung vascular endothelial growth factor ( VEGF ) protein and VEGF receptor 1 ( VEGFR 1 ) protein content were not different between the study groups . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
To account for reproductive failure induced by surgical deletion of paternal accessory sex glands in the golden hamster in vivo , we studied expression of vegf , FLT 1 ( VEGF R 1 ) , FLK 1 ( VEGF R 2 ) , MMP and TGF beta in endometrium of the dam and sired embryos during 5 7 days post coitum by immunohistochemistry , in situ hybridisation , semiquantitative RT PCR and enzyme linked immunosorbent assay . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
VEGFR 1 selective VEGF homologue PlGF is arteriogenic : evidence for a monocyte mediated mechanism . ^^^ Therefore , we used the VEGF homologue placenta growth factor ( PlGF ) , which only binds to VEGFR 1 and VEGF E , which only recognizes VEGFR 2 . ^^^ Evaluation of collateral growth by determining collateral conductance and angiographic scores demonstrated that the VEGFR 1 specific PlGF contributed significantly more to arteriogenesis than the VEGFR 2 specific VEGF E . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Vascular endothelial growth factor ( VEGF ) and its specific receptors FLT 1 and FLK 1 represent an important ligand receptor system involved in angiogenesis and permeability . ^^^ A complete VEGF system was found in endometrial tissue using RT PCR detecting the main VEGF isoform 188 aa , FLT 1 and FLK 1 . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Among these is vascular endothelial growth factor ( VEGF ) , which acts on endothelial cells by binding with 2 specific receptors , VEGFR 1 and VEGFR 2 . ^^^ The aims of this study were to evaluate the expression of VEGF , VEGFR 1 , and VEGFR 2 in digestive endocrine tumors and to examine its correlation with MVD and malignancy . ^^^ A total of 84 specimens from endocrine neoplasms and normal gut and pancreatic tissue were immunohistochemically studied using specific antibodies directed against VEGF , VEGFR 1 , VEGFR 2 , endothelial antigens , and gastroenteropancreatic hormones . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
MATERIAL AND METHODS : We have measured the expression of angiogenesis regulating genes angiopoietin 1 ( Ang 1 ) , angiopoietin 1 ( Ang 2 ) and their receptor Tie 2 , vascular endothelial growth factor ( VEGF ) and its receptors VEGFR 1 and VEGFR 2 in sorted population of CD34+ and CD34+ / CD133+ cells from human cord blood and bone marrow , and in their differentiating progeny , using real time reverse transcriptase polymerase chain reaction . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Expression of vascular endothelial growth factor ( VEGF ) isoforms and its receptors , Flt 1 and KDR , was investigated during the period of peri implantation in mink , a species that displays obligate embryonic diapause . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
In contrast , it was recently reported by two laboratories that Neuropilins can form complexes with the second tyrosine kinase receptor of VEGF , VEGFR 1 . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Here , we confirm that placental soluble fms like tyrosine kinase 1 ( sFlt 1 ) , an antagonist of VEGF and placental growth factor ( PlGF ) , is upregulated in preeclampsia , leading to increased systemic levels of sFlt 1 that fall after delivery . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
There were no significant differences in mRNA expression for VEGF , Flt 1 , angiopoietin 1 , or angiopoietin 2 between BIO TO 2 and F1B control . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
OBJECTIVE : To detect the expression of the vascular endothelial growth factor ( VEGF ) and its receptors , the fms like tyrosine ( flt 1 ) , kinase insert domain containing receptor ( KDR ) in normal human endometrium during menstrual cycle and investigate the functions of VEGF and its receptors for development and differentiation of human endometrium . ^^^ METHODS : Immunohistochemistry and in situ hybridization techniques were used to measure the level of VEGF , flt 1 and KDR protein and mRNA in normal endometrium from 50 women . ^^^ The level of VEGF , protein and mRNA , as well as flt 1 in mid secretory and menstrual phase were highest ( P < 0 . 05 ) . ^^^ CONCLUSIONS : VEGF , flt 1 and KDR , including both protein and mRNA , showed a pronounced menstrual cycle dependent expression in cycling endometrium . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
The aim of the present study was to investigate if the mRNA expression of vascular endothelial growth factor ( VEGF ) , Fms like tyrosine kinase 1 receptor ( Flt 1 ) and kinase insert domain containing receptor ( KDR ) is regulated by shear stress . ^^^ The mRNA expression of VEGF , Flt 1 and KDR was measured with RT PCR . eNOS served as positive control and actin as housekeeping gene . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
BACKGROUND : Vascular endothelial growth factor ( VEGF ) is involved in angiogenesis , wound healing , and inflammation and exerts its effect via tyrosine kinase receptors , fms like tyrosine kinase ( Flt 1 ) and fetal liver kinase ( Flk 1 or KDR ) . ^^^ Physiologic and histologic changes were studied in addition to the mRNA expression of VEGF and its receptors Flt 1 and KDR / Flk 1 by Northern blot and the protein expression of VEGF by Western blot and immunohistochemical staining . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Reverse transcriptase polymerase chain reaction ( RT PCR ) is utilized to measure the VEGF receptors flt 1 , KDR / flk 1 , flt 4 , neuropilin 1 ( NRP 1 ) , and neuropilin 2 ( NRP 2 ) . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
The objective of this study was to elucidate the correlation between the expression of FGF 2 , VEGF , and their receptors FGF receptor 1 ( FGFR 1 ) and VEGF receptor 1 ( Flt 1 ) with microvessel density in synovial tissues of the TMJ . ^^^ These results suggest that the expression of the VEGF / Flt 1 system is involved in angiogenesis in inflamed synovial tissue in the TMJ . . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
VEGF interacts with its receptors , VEGFR 2 and VEGFR 1 , expressed on endothelial and hematopoietic stem cells , and thereby promotes recruitment of these cells to neo angiogenic sites , accelerating the revascularization process . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Expression of VEGF and its receptors Flt 1 and Flk 1 / KDR is altered in lambs with increased pulmonary blood flow and pulmonary hypertension . ^^^ To determine whether this was associated with alterations in VEGF signaling , we investigated vascular changes in expression of VEGF and its receptors , Flt 1 and KDR / Flk 1 , in the lungs of shunted and age matched control lambs during the first 8 wk of life . ^^^ Western blot analysis demonstrated that VEGF , Flt 1 , and KDR / Flk 1 expression was higher in shunted lambs . ^^^ VEGF and Flt 1 expression was increased at 4 and 8 wk of age ( P < 0 . 05 ) . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Vascular permeability factor / vascular endothelial growth factor ( VPF / VEGF ) functions by activating two receptor tyrosine kinases , Flt 1 ( VEGF receptor ( VEGFR ) 1 ) and KDR ( VEGFR 2 ) , both of which are selectively expressed on primary vascular endothelium . ^^^ KDR is responsible for VPF / VEGF stimulated endothelial cell proliferation and migration , whereas Flt 1 down modulates KDR mediated endothelial cell proliferation . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Double staining combined with confocal laser scanning microscopy was used to simultaneously detect the distribution of VEGF receptors ( flt 1 and flk 1 ) in the hippocampal section and isolated neuron . ^^^ Results showed that flt 1 positive staining , but not flk 1 , could be observed on the membrane of the hippocampal neuron in both preparations , suggesting the presence of neuronal membrane VEGF flt 1 receptors in the hippocampus . ^^^ To investigate if the inhibition by VEGF on 1 ( K ) is related to the presence of flt 1 receptors , we further did flt 1 receptor immunostaining for the recorded neurons , which was labeled with Lucifer Yellow during the recording . ^^^ Among nine recorded cells , five showing the inhibition by VEGF had detectable signals for flt 1 receptors on their membrane , whereas the other four showing no inhibition had no flt 1 receptors either . ^^^ The results suggest that VEGF can acutely inhibit 1 ( K ) in the hippocampal neurons probably related to the presence of membrane flt 1 receptors in the neurons . . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Cardiac myofibroblasts : a novel source of vascular endothelial growth factor ( VEGF ) and its receptors Flt 1 and KDR . ^^^ VEGF actions are mediated via two major receptors , Flt 1 and KDR , and hence the expression of these receptors was investigated . ^^^ Flt 1 and KDR expression in myoFb was detected by RT PCR , RNA transcripts were confirmed by northern blot hybridization while western blot confirmed the presence of VEGF , Flt 1 and KDR proteins in myoFb . ^^^ In this study AngII upregulated VEGF and Flt 1 expression in myoFb , but not KDR ; this was mediated predominantly by AT 1 receptor . ^^^ We report for the first time that cardiac myoFb , isolated from the site of infarction express VEGF , its receptors , Flt 1 and KDR , with modulation of VEGF and Flt 1 expression by AngII . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
The specific action of the VEGF on the endothelial cells is mainly regulated by two types of RTK of the VEGF family , VEGFR 1 , or Flt 1 , and VEGFR 2 , or KDR / Flk 1 . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
We performed mutational analysis of FLT 3 , c kit , c fms , vascular endothelial growth factor ( VEGF ) receptors ( Flt 1 , KDR [ kinase domain receptor ] ) , and ras genes in a group of 91 pediatric patients with AML treated on Children ' s Cancer Group clinical trial CCG 2891 . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
To further understand the role of VEGF in mediating angiogenesis during human early pregnancy , we employed a rhesus monkey early pregnancy model to study the temporal and spatial expression of VEGF and its receptors , fms like tyrosine kinase ( Flt ) 1 , and kinase insert domain containing receptor ( KDR ) mRNAs and proteins in the uteri on day 12 , 18 , and 26 of pregnancy using in situ hybridization , RT PCR , and immunohistochemistry . ^^^ The localization of VEGF in the endothelium correlates with the presence of Flt 1 and KDR receptors on vascular structure . ^^^ Expression of VEGF , Flt 1 , and KDR in the epithelial cells also hints some additionally functional roles of VEGF during early pregnancy . . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
In contrast , cytokines and mutants which exclusively bind VEGFR 1 ( human VFM 17 and human VEGF B ) had no effect on invasion and tube formation or on the regulation of gene expression . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
This synergy has been hypothesized to be due to a combination of the following : signaling by PlGF through VEGFR 1 and displacement of VEGF from VEGFR 1 to VEGFR 2 by PlGF , causing increased signaling through VEGFR 2 . ^^^ Synergistic effects observed in that experiment thus appear unlikely to be due to VEGF displacement but to a shift from VEGF VEGFR 1 to PlGF VEGFR 1 complexes and an increase in total VEGFR 1 complexes . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Ets 1 upregulates expression of the vascular endothelial growth factor ( VEGF ) receptor , Flt 1 and we detected increased Flt 1 mRNA levels in ECs co cultured with tumor cells following E 2 treatment . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
VEGF and its receptors , the Fms like tyrosine kinase receptor ( FLT 1 , VEGFR 1 ) and the kinase insert domain containing receptor KDR ( VEGFR 2 / FLK 1 ) were detected immunohistochemically . ^^^ The VEGF receptors FLT 1 and KDR could be detected on endothelial cells of blood vessels . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Placental growth factor ( PlGF ) and vascular endothelial growth factor ( VEGF ) are involved in placental angiogenesis through interactions with the VEGFR 1 and VEGFR 2 receptors . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Sections of testes fixed in Bouin solution and embedded in paraffin were subjected to immunofluorescent staining with specific antibodies against VEGF , and its receptors , VEGFR 1 ( Flt 1 ) and VEGFR 2 ( Flk 1 ) . ^^^ Thus , VEGF may play a potential role in regulating the initial stages of the process of spermatogonial proliferation through VEGFR 2 and spermiogenesis through VEGFR 1 . . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
The biological effects of VEGF are mediated by two receptor tyrosine kinases ( RTKs ) , VEGFR 1 and VEGFR 2 , which differ considerably in signaling properties . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
To determine the involvement of vascular endothelial growth factor ( VEGF ) and its receptors Flk 1 and Flt 1 in capillary growth in ischaemic skeletal muscle , extensor digitorum longus muscles from hindlimbs of Sprague Dawley rats were studied at 1 , 2 and 5 week intervals after iliac artery ligation . ^^^ During more sustained ischaemia ( femoral ligation 3 weeks after iliac ligation ) , VEGF protein level at 5 weeks was even higher , but Flt 1 and Flk 1 were unchanged from control levels and no capillary growth occurred . ^^^ Intermittent electrical stimulation ( 10 Hz , 7x15 min / day ) of these ischaemic muscles between weeks 3 5 did not elevate VEGF further , but increased Flk 1 by 32 % , decreased Flt 1 by 71 % , and led to significant capillary growth . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Tissues adjacent to the infusion / wound site were analyzed for specific vascular and astroglial protein markers and proliferation , necrosis / apoptosis ( via TUNEL staining ) , VEGF , the VEGF receptors flt 1 and flk 1 , and bFGF expression using immunohistochemistry and semi quantitative RT PCR . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
OBJECTIVE : To study the expression of vascular endothelial cell growth factor ( VEGF ) and its receptor FLT 1 , FLK 1 mRNA in breast cancer tissues and their correlation with clinicopathological factors . ^^^ METHODS : The expression of VEGF and and its receptor FLT 1 , FLK 1 mRNA were analyzed by reverse transcription polymerase chain reaction ( RT PCR ) in the specimens from 47 patients with breast cancer and 11 patients with benign breast disease . ^^^ Moreover , no correlation was observed between the expression of VEGF 121 , VEGF 165 , FLT 1 , FLK 1 mRNA in breast cancer tissues and patients ' age , tumor size , lymph node metastasis , tumor stages , estrogen or progesterone receptor status . ^^^ CONCLUSION : The expression of VEGF and its receptor FLT 1 , FLK 1 mRNA were up regulated in breast cancer tissues , suggesting its important role in angiogenesis of breast cancer . . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Role of PlGF in the intra and intermolecular cross talk between the VEGF receptors Flt 1 and Flk 1 . ^^^ Here we report that placental growth factor ( PGF , also known as PlGF ) regulates inter and intramolecular cross talk between the VEGF RTKs Flt 1 and Flk 1 . ^^^ Activation of Flt 1 by PGF resulted in intermolecular transphosphorylation of Flk 1 , thereby amplifying VEGF driven angiogenesis through Flk 1 . ^^^ Even though VEGF and PGF both bind Flt 1 , PGF uniquely stimulated the phosphorylation of specific Flt 1 tyrosine residues and the expression of distinct downstream target genes . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
The aim of the present study was to evaluate vascular endothelial growth factor ( VEGF ) , fms like tyrosine kinase 1 ( flt 1 ) , and fetal liver kinase ( flk 1 ) expression in the heart of experimental diabetic rats . ^^^ RT PCR was also performed for VEGF receptors flk 1 and flt 1 . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
PTK787 / ZK 222584 ( PTK / ZK ) is an oral potent and selective inhibitor of the vascular endothelial growth factor ( VEGF ) mediated Flt 1 and KDR receptor tyrosine kinases . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Stimulation by either 50 ng / ml VEGF A 165 or by 24 h of anoxia resulted in an altered pattern of receptor expression for VEGF R 2 ( Flk 1 ) , VEGF R 3 ( Flt 4 ) , neuropilin 1 , and neuropilin 2 , whereas VEGF R 1 ( Flt 1 ) remained unexpressed . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
We then measured levels of tie 2 , vascular endothelial growth factor ( VEGF ) , another factor associated with angiogenesis , and the soluble VEGF receptor Flt 1 ( sFlt 1 ) in 75 patients with coronary artery disease [ 25 with acute myocardial infarction ( AMI ) , 25 with acute coronary syndromes ( ACS ) and 25 with stable angina ] and 25 healthy controls . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
In addition , to confirm extraexpression of the VEGF gene after injection , we assessed the expression of two isoforms of VEGF ( VEGF 120 and VEGF 164 ) in granulosa cells and expression of fms like tyrosine kinase ( Flt 1 ) , expression of fetal liver kinase ( Flk 1 ) , and density of capillary networks in theca cells . ^^^ Granulosa cells and thecal tissues in the antral follicles ( diameter , > 4 mm ) were collected to detect the mRNA expression of VEGF isoforms in the granulosa cells and of Flt 1 and Flk 1 in the thecal tissues by semiquantitative reverse transcription polymerase chain reaction . ^^^ The Flt 1 , but not the Flk 1 , mRNA expression show a tendency toward increasing in the thecal tissues of antral follicles in the ovaries injected with VEGF gene fragments . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
We challenged this thesis by employing both VEGF ( 165 ) and vascular endothelial growth factor receptor 1 ( VEGFR 1 ) ( for competitive inhibition of VEGF signal transduction ) in different experimental settings of an ischemic rat flap model . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
In the present study , green tea extract ( GTE ) was tested for its ability to inhibit cell viability , cell proliferation , cell cycle dynamics , vascular endothelial growth factor ( VEGF ) and expression of VEGF receptors fms like tyrosine kinase ( Flt 1 ) and fetal liver kinase 1 / Kinase insert domain containing receptor ( Flk 1 / KDR ) in vitro using human umbilical vein endothelial cells ( HUVECs ) . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
We have previously reported the locoregional ( i . p . ) delivery of adenovirus mediated antiangiogenic soluble FLT 1 ( sFLT 1 ; a naturally encoded potent VEGF antagonist ) gene therapy to inhibit VEGF action in a murine ovarian carcinoma model . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
The levels of mRNA for Flt 1 , a VEGF specific receptor , and those for ORP 150 , a chaperone essential for the secretion of mature VEGF , increased in whole plantaris muscles ( 108 and 92 % , respectively , P < 0 . 05 ) . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Vascular endothelial growth factor receptor 1 ( VEGFR 1 ) is a tyrosine kinase receptor for several growth factors of the VEGF family . ^^^ Vascular endothelial growth factor receptor 1 ( VEGFR 1 ) is a tyrosine kinase receptor for several growth factors of the VEGF family . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
In contrast to VEGF and its receptor VEGFR 2 , PlGF and its receptor VEGFR 1 have been largely neglected and therefore their potential for therapy has not been previously explored . ^^^ In this review , we describe the molecular properties of PlGF and VEGFR 1 and how this translates into an important role for PlGF in the angiogenic switch in pathological angiogenesis , by interacting with VEGFR 1 and synergizing with VEGF . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
The action of VEGF is mediated by two different high affinity receptors VEGF receptor 1 ( VEGFR 1 ; flm like tyrosine kinase 1 ; flt 1 ) and VEGFR 2 ( kinase insert domain containing receptor ; flk 1 ) predominantly located on the vascular endothelium . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Of the three VEGF receptors ( VEGFR ) , VEGFR 1 and 2 are expressed on blood vessels ; VEGFR 2 is found also on lymphatic vessels . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Vascular endothelial growth factor ( VEGF ) and its receptors VEGFR 1 and VEGFR 2 have been implicated as key players in vascular remodelling and placentation . ^^^ Implantation in marmosets occurs at day 11 of pregnancy ; hence , these time points were chosen so that the peri implantation period and very early pregnancy could be studied . mRNAs for VEGF , VEGFR 1 and VEGFR 2 , angiopoietin 1 , angiopoietin 2 and their receptor Tie 2 were localized and quantified by in situ hybridization . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
METHODS : 70 surgical samples of central nervous system cavernous hemangiomas and 20 normal brain vessel samples from patients of epilepsy and open craniocerebral trauma were stained immunohistochemically with CD 34 , a SMA ; VEGF , Flt 1 ; and TGFa , Ki 67 respectively . ^^^ VEGF , Flt 1 and TGFa were not expressed obviously in the normal brain tissues . 47 and 50 out of the 70 cavernous hemangioma specimens were positively stained for CD 34 and a SMA respectively , and their expression was less continuous . 68 and 44 out of the 70 cavernous hemangioma specimens were positively stained for VEGF and Flt 1 respectively with diffuse distribution . 68 cavernous hemangioma specimens were positively stained for TGF a . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Here , we report that the gene expression of the VEGF receptor Flt 1 ( vascular endothelial growth factor receptor 1 ) was down regulated by the reversible proteasome inhibitor MG 262 in explant cultures of the developing chicken pecten oculi , a vascular organ consisting of endothelial cells , pericytes , and macrophages . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Immunohistochemistry for VEGF and Flt 1 was similar in fetuses , preterm infants , and term infants : for VEGF mostly in bronchial epithelium and alveolar macrophages , and for Flt 1 mostly in vascular endothelial cells and bronchial epithelial cells . ^^^ In patients with BPD , and PPHN , staining for VEGF and Flt 1 appeared also in Type 2 pneumocytes . ^^^ The persistent expression of VEGF and Flt 1 during the fetal and neonatal period supports a physiological role for VEGF in human lung development . ^^^ In PPHN , that more cell types express VEGF and Flt 1 , and the tendency toward a higher concentration of pulmonary VEGF may represent enhanced production of VEGF in response to impaired endothelial function . . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
BACKGROUND : It has been shown that expression of the potent angiogenic factor , vascular endothelial growth factor ( VEGF ) , and its receptors , flt 1 ( VEGFR 1 ) and KDR / Flk 1 ( VEGFR 2 ) , increased during the development of liver fibrosis . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Tumour cells are the main source of VEGF in GBMs whereas VEGF receptors ( VEGFR 1 , its soluble form sVEGFR 1 , VEGFR 2 and neuropilin 1 ) are expressed predominantly by endothelial cells . ^^^ We used quantitative RT PCR , Western blot , gelatin zymography and immunohistochemistry to study the expression of VEGF , VEGFR 1 , VEGFR 2 , sVEGFR 1 , neuropilin 1 , MT 1 MMP , MMP 2 , MMP 9 and TIMP 2 in 20 human GBMs and 5 normal brains . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Samples were analyzed for VEGF , VEGFR 1 and VEGFR 2 mRNA expression using real time PCR and protein expression using Western Blot analysis . ^^^ Muscles from both extremities showed significantly increased expression of VEGF and its cognate receptors VEGFR 1 / 2 . ^^^ Interestingly VEGF and VEGFR 1 levels at the non distracted side were significantly higher than at the distracted side . ^^^ These findings indicate that callus distraction results not only in locally increased expression of VEGF and its receptors , but leads also to increased VEGF and VEGFR 1 / 2 levels at distant sides and might therefore be responsible for the observed growth phenomena during callus distraction . . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Such an autocrine loop is supported by constitutive VEGF receptor ( Flk 1 ) tyrosine phosphorylation , Flk 1 and Flt 1 nuclear localization , and mitogen activated protein kinase activation . beta catenin was also found to exhibit significant nuclear localization and constitutively associate with Flk 1 and Flt 1 in EOMA cells but much less so in HUVEC , and immunoprecipitated Flk 1 was able to phosphorylate purified beta catenin in an immune complex kinase assay . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
The VEGF receptor 1 ( VEGFR 1 / Flt 1 ) mediates monocyte migration towards VEGF and regulates the levels of available VEGF through ligand entrapment . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
In situ hybridization demonstrated presence of mRNA for VEGF receptors Flt 1 , fetal liver kinase 1 , neuropilin 1 and 2 in several important cellular compartments of the spinal cord . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
METHODS : Paraffin embedded tissues of 39 NSGCTs ( 19 organ confined NSGCTs , pathological stage 1 and 20 NSGCTs with metastasis , pathological stage 2 ) were immunohistochemical stained with antibodies for vascular endothelial growth factor ( VEGF ) with the ligands flt and flk , for the angiogenetic factor ets 1 as well as for endothelial markers CD 34 and CD 105 . ^^^ There was no significant difference in the expression of VEGF , flt or flk in metastatic vs . organ confined tumors and their different histological subtypes seen . ^^^ DISCUSSION : VEGF and its receptors flt and flk seem not be involved in the progress of metastatic development of NSGCTs . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
The vascular endothelial growth factor ( VEGF ) tyrosine kinase receptors KDR and Flt 1 are targets of current interest in anticancer drug research . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Expression of the VEGF receptor Flk 1 , but not Flt 1 , was increased in colonic tissue of azoxymethane treated rats compared with control rats . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
We investigated whether acute systemic exercise increases vascular endothelial growth factor ( VEGF ) , VEGF receptor ( KDR and Flt 1 ) mRNA , and VEGF protein in sedentary humans . ^^^ Acute exercise significantly increased VEGF mRNA at 2 and 4 h and increased KDR and Flt 1 mRNA at 4 h postexercise . ^^^ The sustained increase in VEGF mRNA through 4 h and the increases in KDR and Flt 1 at 4 h are different from their respective time course responses in rats . ^^^ These results provide evidence in humans that 1 ) VEGF , KDR , and Flt 1 mRNA are increased by acute systemic exercise ; 2 ) the time course of the VEGF , KDR , and Flt 1 mRNA responses are different from those previously reported in rats ( Gavin TP and Wagner PD . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Several growth factors and specific receptors , e . g . vascular endothelial growth factor ( VEGF ) , PlGF ( placenta growth factor ) and the soluble VEGF 1 receptor ( VEGFR 1 ) play essential roles in the process . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Vascular endothelial growth factor ( VEGF ) is known to play a predominant role in tumor angiogenesis and metastasis formation that is mediated by its interactions with two tyrosine kinase receptors , VEGFRI ( Flt 1 ) and VEGFRII ( KDR ) . ^^^ A novel peptide , SP5 . 2 , which selectively binds Flt 1 and inhibits a broad range of VEGF mediated events , was identified using a phage display library screening . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Therefore , VEGF and its receptors VEGFR 1 and VEGFR 2 are prime targets for anti angiogenic intervention which is thought to be one of the most promising approaches in cancer therapy . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
We have shown a cyclic regulation of the soluble VEGF receptor , sflt , in human endometrium and have detected the expression of the transmembraneous VEGF receptors , Flt 1 and kinase insert domain containing receptor ( KDR ) throughout the menstrual cycle . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
VEGF and VEGF receptor , Flt 1 , expression was observed in periprosthetic tissues surrounding loosened total joint implants . ^^^ RESULTS : Double immunofluorescent staining showed that VEGF and Flt 1 co localized with cells positive for the macrophage marker , CD11b , in the periprosthetic tissues . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Expression and localization of mRNAs for vascular endothelial growth factor ( VEGF ) , VEGF receptor 1 ( Flt ) and VEGF receptor 2 ( KDR ) ( VEGFR 1 and VEGFR 2 , respectively ) were investigated in pig corpora lutea . ^^^ Northern blot analysis of total RNA indicated hybridization of pig VEGF , VEGFR 1 and VEGFR 2 cDNA probes to mRNA transcripts of approximately 3 . 9 , 7 . 0 and 5 . 0 kb , respectively . ^^^ Relative hybridization signals of expression of VEGF mRNA appeared to be constant ; however , expression of VEGFR 1 mRNA was low on day 4 , increased on day 7 , and was higher on days 10 , 13 and 15 ( P < 0 . 05 , compared with day 4 ) . ^^^ In situ hybridization revealed that VEGF mRNA was localized predominantly in large luteal cells , whereas both VEGFR 1 and VEGFR 2 were localized to small cells . ^^^ Although the expression of VEGF mRNA was unchanged during the luteal phase , variations in the expression of VEGFR 1 and VEGFR 2 mRNAs indicate that differential regulation of expression of the VEGF receptors may play a role in the control of VEGF mediated vascular growth at different phases of development and maturation of the pig corpus luteum . . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Using immunohistochemical techniques , we assessed the vascular density and distribution of angiogenesis ( FVIII ) and vascular endothelial growth factor ( VEGF ) expression as well as the expression of 2 VEGF receptors , Flt 1 and Flk 1 , in 55 nonrheumatic and 6 control aortic valves . ^^^ Diseased valves showed distinct VEGF , Flt 1 , Flk 1 , and eNOS positivity of activated endothelial , stromal fusiform myofibroblastic , and histocytic cells . ^^^ A similar tendency was also observed for VEGF , but not for Flt 1 . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
To gain insight into how this bacteria induces angiogenesis in vivo , we performed in situ hybridization of clinical specimens of verruga peruana for the angiogenesis factors vascular endothelial growth factor ( VEGF ) , its receptors VEGFR 1 and VEGFR 2 , and angiopoietin 2 . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
In the present study immunohistochemical analysis showed that , in normoperfused mouse hindlimb , VEGF and its receptors Flk 1 and Flt 1 were expressed mostly in quiescent satellite cells . ^^^ At day 3 and day 7 after the induction of ischemia , Flk 1 and Flt 1 were expressed in regenerating muscle fibers and VEGF expression by these fibers was markedly enhanced . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Plasma angiopoietin 1 , angiopoietin 2 and Tie 2 in breast and prostate cancer : a comparison with VEGF and Flt 1 . ^^^ These growth factors include angiopoietin 1 ( Ang 1 ) , angiopoietin 2 ( Ang 2 ) and vascular endothelial growth factor ( VEGF ) , which act through receptors Flt 1 and Tie 2 . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
To dissect the molecular basis of this process , we analyzed localization of proangiogenic vascular endothelial growth factor ( VEGF ) , its receptor vascular endothelial growth factor receptor 1 ( VEGFR 1 , Flt 1 ) , of downstream VEGF effectors matrix metalloproteinase 1 ( MMP 1 ) and connective tissue growth factor ( CTGF ) , and of VEGF interacting antiangiogenic thrombospondin 1 and independent angiostatin in brains of patients who died with CM and controls by immunohistochemistry and Western blotting experiments . ^^^ Most prominently , we detected more VEGF ( + ) astrocytes in CM patients and deposition of Flt 1 in Drck ' s granulomas . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
We studied the expression of VEGF and its receptors ( VEGFR 1 or Flt 1 and VEGFR 2 or Flk 1 / KDR ) by myeloma cell lines and plasma cells isolated from patients , using different methods . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
We report here that Leydig cell tumours are characterised by an increased level of vascular endothelial growth factor ( VEGF ) in testicular veins , the presence of VEGF mRNA and of its receptor , KDR , and an absence of detectable VEGF receptor Flt 1 , in blood vessels of tumour marginal zones and of peri tumour areas . ^^^ These results suggest an autocrine role of VEGF on endothelial cells of tumour blood vessels in a region specific manner and implicate that VEGF interactions with KDR , in the absence of Flt 1 , may be involved in vascular destabilisation . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Vascular endothelial growth factor ( VEGF ) regulates blood vessel formation by binding to the receptor tyrosine kinases VEGF receptor 1 ( Flt 1 ) or VEGF receptor 2 ( KDR ) and to the structurally unrelated neuropilins . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
A naturally occurring soluble VEGFR 1 has been discovered in the supernatant from endothelial cells and at present appears to be the key regulator for the availability of VEGF secreted from different cells and tissues . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
The localization of VEGF bound to the endothelium correlates with the presence of flt 1 and flk / KDR receptors on vascular structures , including capillary strands which have not yet formed a lumen , present during the mid secretory period , which corresponds to a high estroprogestin influence and to implantation . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Immunohistochemical techniques were used to evaluate VEGF protein localization with rabbit polyclonal anti rat VEGF , VEGF receptor ( VEGFR ) expression with rabbit polyclonal antibodies to VEGFR 1 and 2 , microvascular density with mouse monoclonal anti rat CD 31 , and astrocytic reactivity with polyclonal anti glial fibrillary acidic protein , in cerebral cortical tissue of the right middle cerebral artery territory . ^^^ Consistent with the VEGF protein expression findings , up regulation of VEGFR 1 but not VEGFR 2 expression on endothelial cells in the model brains was observed . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Our data reveal that the EMT results in the increased expression of both VEGF and Flt 1 , a tyrosine kinase VEGF receptor , and that the survival of these cells depends on a VEGF / Flt 1 autocrine pathway . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Blockade of vascular endothelial growth factor receptor 1 ( VEGF RI ) , but not VEGF RII , suppresses joint destruction in the K / BxN model of rheumatoid arthritis . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
The aim of this study was to evaluate the expression of VEGF mRNA and receptor flt 1 mRNA ( VEGF R 1 ) in a clinical material of RCCs compared with clinicopathological variables and serum VEGF levels . ^^^ Expression of mRNA for VEGF 121 , VEGF 165 and flt 1 were analysed using quantitative RT PCR . ^^^ Papillary RCC had significantly lower VEGF 121 and flt 1 mRNA levels compared with conventional RCC ( p=0 . 001 ) . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
This study compares the mRNA expression of vascular endothelial growth factor ( VEGF ) and its receptors ( KDR and flt 1 ) in the hydrosalpinx with that in the healthy oviduct . ^^^ RESULTS : mRNA expression of VEGF and its receptor flt 1 in the hydrosalpinx was significantly higher than that in the healthy oviduct , but no significant difference was demonstrated for the KDR receptor . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Immunostaining with vascular endothelial growth factor ( VEGF ) and anti flt 1 / VEGF receptor 1 antibodies showed distinct tissue patterns . ^^^ VEGF immunoreactivity occurred mainly in the processes of the tumor astrocytes in PA ; the opposite was observed in AA . flt 1 / VEGFR 1 was detected in the tumor cells of AA but not in those of PA . ^^^ The expression of both flt 1 and VEGF by AA tumor cells also suggests a possible autocrine growth promoting function for VEGF in addition to its role as paracrine pro angiogenic growth factor for activated ECs , thus making anti angiogenesis an attractive therapeutic target in the treatment of AA . . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
STUDY DESIGN : We measured mRNA expression of vascular endothelial growth factor ( VEGF ) , angiopoietin 1 and 2 ( Ang 1 and Ang 2 ) , their receptors VEGFR 1 , VEGFR 2 , Tie 2 , fibroblast growth factor 2 ( FGF 2 ) , and its receptor FGF 2R in placental tissue of diabetes type 1 patients , in normal term placenta , and endometrium of non pregnant women by real time reverse transcriptase PCR . ^^^ We did not detect a significant difference in the expression of Ang 1 , Ang 2 , Tie 2 , VEGF , VEGFR 1 , VEGFR 2 , FGF 2 , and FGF 2R in normal and diabetes type 1 placenta . ^^^ CONCLUSION : These data show differential expression of Ang 2 , VEGFR 1 , VEGF , and VEGFR 2 in placenta and endometrium . ^^^ The expression of Ang 1 , Ang 2 , Tie 2 , VEGF , VEGFR 1 , VEGFR 2 , FGF 2 , and FGF 2R was not different in normal and type 1 diabetes placenta . . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Prognostic significance of VEGF receptors , VEGFR 1 ( Flt 1 ) and VEGFR 2 ( KDR / Flk 1 ) in breast carcinoma ] . ^^^ OBJECTIVES : The aim of this study was to investigate the prognostic value of vascular endothelial growth factor ( VEGF ) receptors , VEGFR 1 ( Flt 1 ) and VEGFR 2 ( KDR / Flk 1 ) in breast carcinoma . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
METHODS : Normal and arterialized vein graft segments were evaluated by reverse transcription polymerase chain reaction ( RT PCR ) for expression of VEGF R 1 ( flt ) , VEGF R 2 ( KDR ) , and neuropilin 1 . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Vascular endothelial growth factor ( VEGF 165 ) exhibits multiple effects via the activation of two distinct endothelial receptor tyrosine kinases : Flt 1 ( fms like tyrosine kinase 1 ) and KDR ( kinase insert domain containing receptor ) . ^^^ KDR shows strong ligand dependent tyrosine phosphorylation in comparison with Flt 1 and mainly mediates the mitogenic , angiogenic , and permeability enhancing effects of VEGF 165 . ^^^ A receptor binding assay revealed that snake venom VEGFs bound to KDR IgG with high affinity ( Kd = approximately 0 . 1 nm ) as well as to VEGF 165 but did not interact with Flt 1 , Flt 4 , or neuropilin 1 at all . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
In the present study , we tested the antiangiogenic and antitumor effects of soluble VEGF receptor 1 [ soluble Flt ( sFlt ) 1 ] on the growth of follicular thyroid carcinoma ( FTC ) . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Northern blot analyses of basic fibroblast growth factor , VEGF , Flt 1 and Flk 1 mRNA expression supported these findings and showed a dose dependent decrease in EtOH treated cultures compared to controls . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
The aim of this study is to determine the localization of VEGF and its receptors , Flt 1 and KDR , and bFGF expression in the rat ovary and to evaluate their distributions throughout the different follicular stages . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
The IR levels of HIF 1alpha and VEGF reached plateau at 4 weeks , and the IRs of VEGFR 1 and VEGFR 2 were strongly positive in the hypoxic group . ^^^ Hence , HIF 1 may activate the expression of VEGF and VEGFR 1 in the CB and the expression of VEGF in the chemoreceptors may play a paracrine role in the vascular remodeling during chronic hypoxia . . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Chimeric protein , of which the extracellular domain of VEGF receptor 1 ( VEGFR 1 ) fused to immunoglobulin Fc , inhibited FGF 2 induced cell extension , resulting in decreased capillary morphogenesis . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Plasma concentrations of von Willebrand factor ( vWf , a marker of endothelial damage / dysfunction ) , vascular endothelial growth factor ( VEGF , associated with angiogenesis ) , soluble VEGF receptor Flt 1 ( sFlt 1 ) , and tissue factor ( TF , a key component of coagulation ) were measured by an enzyme linked immunosorbent assay . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Additional studies demonstrated similar levels of both vascular endothelial growth factor ( VEGF ) mRNA and protein and enhanced levels of VEGF receptor 1 ( Flt 1 ) mRNA in PAI 1 deficient cells relative to wild type cells . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
We studied the effects of VEGF on human aortic endothelial cells ( HAECs ) , which express VEGFR 1 and VEGFR 2 , but not VEGFR 3 , in the absence or presence of ZM 323881 . ^^^ Inhibition of VEGFR 2 blocked activation of extracellular regulated kinase , p 38 , Akt , and endothelial nitric oxide synthetase ( eNOS ) by VEGF , but did not inhibit p 38 activation by the VEGFR 1 specific ligand , placental growth factor ( PIGF ) . ^^^ Inhibition of VEGFR 2 thus blocked all VEGF induced endothelial cellular responses tested , supporting that the catalytic activity of VEGFR 2 is critical for VEGF signaling and / or that VEGFR 2 may function in a heterodimer with VEGFR 1 in human vascular endothelial cells . . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Both ERK activation and fibronectin secretion appear to be mediated through the VEGF receptor flt 1 , as evidenced by the effects of the flt 1 specific ligand placenta growth factor . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Major findings were upregulation of several genes associated with cell cycle progression , including cyclin E and D ; downregulation of cyclin dependent kinase inhibitors p 21 and p 27 , cyclin dependent kinases 2 , 5 , and 6 , and monocyte chemoattractant protein 1 ; and upregulation of growth factors , including vascular endothelial growth factor ( VEGF ) and vascular endothelial growth factor receptor 1 ( VEGFRI ) , endothelial growth factor ( EGF ) and hepatic derived growth factor ( HDGF ) . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Immature B cell malignancies synthesize VEGF , VEGFR 1 ( Flt 1 ) and VEGFR 2 ( KDR ) . ^^^ We now show that transcripts of VEGF , and its receptors VEGFR 1 and VEGFR 2 are concomitantly expressed in both ALL cell lines and primary pre B ALL . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
The biological activity of VEGF is mediated by two different receptor tyrosine kinases : VEGFR 2 and VEGFR 1 . ^^^ The soluble form of VEGFR 1 is likely to be a negative regulator of VEGF availability , but the physiological role of sVEGFR 2 is still unclear . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Because gene knock outs of VEGF and its receptors flk 1 and flt 1 result in early embryonic lethality , determining roles for VEGF in CNS development has been particularly difficult . ^^^ Antisense oligodeoxynucleotides to flk 1 , but not flt 1 , inhibited neuritic outgrowth , whereas inhibitors of the signaling pathways MEK 1 and P 13 AKT both abrogated VEGF induced growth . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
TGF beta 1 potently induces VEGF receptor 1 ( VEGFR 1 ) expression in endothelial cells and thereby prevents oxygen induced vessel loss in vivo . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
As vascular endothelial growth factor ( VEGF ) is a potent mitogen for vascular endothelial cells , here we have investigated the mRNA and protein expression of VEGF and the mRNA expression of its two receptors ( Flt 1 and Flk 1 / KDR ) in pheochromocytomas tissue . ^^^ Flk 1 / KDR , and Flt 1 levels of mRNA were also increased markedly in tumors and correlated with levels of VEGF mRNA . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
SUMMARY : The angiogenic role of vascular endothelial growth factor ( VEGF ) receptors , flk 1 and flt 1 , and their downstream signaling pathways , MAPK / ERK and PI 3 kinase , were examined in a fetal rat cortical explant model after exposure to exogenous VEGF . ^^^ Treatment with VEGF resulted in substantial neovascularization characterized by increased vascular flk 1 receptor expression , whereas flt 1 receptor protein expression was absent . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
MATERIALS AND METHODS : Using RT PCR we examined the expression of VEGF and its receptors , KDR , Flt 1 , NP 1 and NP 2 , in 8 human prostate cancer cell lines and then correlated the expression patterns with the ability of these cell lines to invade and migrate through membranes in a modified Boyden Chamber assay in the presence and absence of VEGF . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Neutralizing VEGF bioactivity with a soluble chimeric VEGF receptor protein flt ( 1 3 ) IgG inhibits testosterone stimulated prostate growth in castrated mice . ^^^ METHODS : Adult male mice were castrated and after 1 week treated with testosterone and vehicle , or with testosterone and a soluble chimeric VEGF receptor flt ( 1 3 ) IgG protein . ^^^ The testosterone stimulated increase in epithelial cell proliferation was unaffected by flt ( 1 3 ) IgG , but endothelial and epithelial cell apoptosis were increased in the anti VEGF compared to the vehicle treated groups . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
VEGF , Flt 1 , Flk 1 . ^^^ To estimate the dependence transcriptional activity of Flt 1 and Flk 1 on VEGF gene expression Spearman ' s correlation rank was performed . ^^^ RESULTS : The comparison of transcriptional activity of VEGF and its receptors revealed significant correlation between increase in the number of Flt 1 mRNA copies and enhanced mRNA expression of VEGF 121 ( p < 0 . 05 ) , VEGF 145 ( p < 0 . 05 ) , VEGF 165 ( p < 0 . 05 ) , VEGF 183 ( p < 0 . 05 ) , VEGF 189 ( p < 0 . 05 ) , and VEGF 206 ( p < 0 . 05 ) . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Here we report the crystal structure of PlGF bound to the second immunoglobulin like domain of VEGFR 1 at 2 . 5 A resolution and compare the complex to the closely related structure of VEGF bound to the same receptor domain . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
The aim of the present study was to investigate the expression of the VEGF receptors VEGFR 1 , VEGFR 2 , and VEGFR 3 on B CLL cells , singly and combined . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Expression of VEGF receptors ( flt 1 , flk 1 ) , endoglin and hypoxia inducible factor 1alpha ( HIF 1alpha ) was assessed by quantitative RT PCR and for endoglin also by immunohistochemistry . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Placental growth factor ( PlGF ) is a member of the vascular endothelial growth factor ( VEGF ) family that binds specifically to Flt 1 . ^^^ These findings show for the first time that VEGF and PlGF induce mRNA expression of the transcription factors FosB and c Fos , and suggest that these factors may play a role in the biological responses mediated by PlGF and Flt 1 . . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
VEGF 164 ( 165 ) as the pathological isoform : differential leukocyte and endothelial responses through VEGFR 1 and VEGFR 2 . ^^^ VEGF ( 164 ) was also more potent at inducing the chemotaxis of monocytes , an effect that was mediated by VEGFR 1 . ^^^ In an immortalized human leukocyte cell line , VEGF ( 165 ) was found to induce tyrosine phosphorylation of VEGFR 1 more efficiently . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
The corneas of the C57BL / 6J mice demonstrated 1 . 8 , 1 . 5 , and 1 . 7 fold increased mRNA levels for Flt 1 , VEGF , and bFGF , respectively ( P < 0 . 02 ; P < 0 . 04 ; P < 0 . 02 ) ; however , TSP 1 expression was increased 2 . 4 fold compared with 129S3 / SvIM ( P < 0 . 0004 ) . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
METHODS : Expression of VEGF and its receptors ( VEGFR 1 and VEGFR 2 ) was examined in passive Heymann nephritis ( PHN ) and puromycin aminonucleoside nephrosis ( PAN ) , by immunohistochemistry , in situ hybridization , Northern and Western blotting . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Immunohistochemical analysis of the cultures demonstrated the presence of VEGFR 2 after VEGF treatment , as well as the expression of the VEGF receptor VEGFR 1 ( flt 1 ) . ^^^ Treatment of the cultures with antisense oligonucleotides against VEGFR 2 , but not against VEGFR 1 , abolished the effect of VEGF on the length of Map 2 ( + ) processes . ^^^ RT PCR analyses of Map 2 and VEGFR 1 indicated that mRNAs of these two genes are upregulated in the presence of VEGF . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Primary varicose veins : altered transcription of VEGF and its receptors ( KDR , flt 1 , soluble flt 1 ) with sapheno femoral junction incompetence . ^^^ Our aim was to examine transcription of genes for VEGF ( VEGF ( 121 ) / VEGF ( 165 ) ) and its receptors ( KDR , flt 1 , s . flt 1 ) in VVs , in relation to underlying venous incompetence . ^^^ RESULTS : VEGF ( 121 ) / ( 165 ) , KDR and flt 1 transcription was elevated in VVs overall ( p < 0 . 001 ) , and in VVs with an incompetent SFJ ( p < 0 . 001 ) , but not when the SFJ was functional ; s . flt 1 was unaltered . ^^^ Position below the SFJ correlated with increased transcription of s . flt 1 when the SFJ was incompetent ( p < 0 . 04 ) , and s . flt 1 and VEGF ( 121 ) when the segment was incompetent ( p < 0 . 03 ) . ^^^ Altered VEGF ( 121 ) and s . flt 1 transcription may be an early event in varicogenesis . . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
We investigated the genetic basis of VEGF expression in patients with type 1 ( onset before age 40 y ) chronic plaque psoriasis compared to healthy controls and also measured plasma levels of VEGF and its receptors flt 1 and KDR . ^^^ Plasma levels of VEGF and flt 1 were significantly detectable in patients with psoriasis compared with controls ( p < 0 . 001 ) ; by contrast , mean plasma levels of KDR in psoriatic patients were comparable with controls . ^^^ VEGF , flt 1 , and KDR could provide attractive targets for future psoriasis therapy . . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
RESULT ( S ) : Immunohistochemical study localized VEGF R , both KDR and flt 1 , in the oviduct luminal epithelium , smooth muscle cells as well as blood vessels within the oviduct . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Changes of mRNA levels of VEGF , Flt 1 , interleukin 6 , and osteoclast differentiation factor ( ODF / TRANCE / RANKL ) in bone tissue were measured by reverse transcriptase polymerase chain reaction . ^^^ VEGF mRNA , Flt 1 mRNA , interleukin 6 mRNA , and RANKL mRNA levels in bone tissue were elevated in OVX mice over that in sham operated mice . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Two distinct receptors for VEGF , the tyrosine kinase receptors VEGFR 1 ( Flt 1 ) and VEGFR 2 ( Flk 1 / KDR ) , have been identified . ^^^ Functions of the VEGF receptor 1 ( FLT 1 ) in the vasculature . ^^^ Transfection studies could demonstrate biological activities for the Flk 1 / KDR , but not for the Flt 1 receptor , which led to the hypothesis that Flt 1 is a decoy receptor for VEGF . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Elevated levels of the soluble vascular endothelial growth factor receptor 1 , which binds and inhibits circulating forms of VEGF were identified in patients with preeclampsia ; rats injected with this soluble receptor develop hypertension , endotheliosis and proteinuria , similar to the lesion seen in podocyte specific haploinsufficient VEGF mice . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Combined systemic treatment with blocking antibodies against VEGF receptor 1 ( VEGFR 1 ) and VEGFR 2 potently inhibited inflammation and also decreased lymphatic vessel size . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Limited data suggest that excess circulating soluble fms like tyrosine kinase 1 ( sFlt 1 ) , which binds placental growth factor ( PlGF ) and vascular endothelial growth factor ( VEGF ) , may have a pathogenic role . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Western blot analyses were used to evaluate HIF 1alpha , HIF 1beta , HIF 2alpha , von Hippel Lindau protein , VEGF , Flt 1 , enolase , and GAPDH . ^^^ Placental expression of HIF 1 mediated proteins VEGF , Flt 1 , enolase , and GAPDH were unchanged at high vs . low altitude . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Interestingly , levels of VEGF , VEGF receptors ( KDR , Flt 1 ) , endothelial nitric oxide synthase , phosphorylated Akt ( pAkt ) , estrogen receptor alpha , aromatase , and capillary density in sham operated SHRSP were remarkably down regulated , whereas androgen receptor levels were up regulated , compared with age matched genetic control , Wistar Kyoto rats . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Furthermore , using mouse wound healing models , we demonstrated that adenovirus mediated delivery of EPAS 1 gene significantly induced the expression of VEGF , Flt 1 , Flk 1 , and Tie 2 mRNA at the wound site and promoted mature angiogenesis . ^^^ In conclusion , EPAS 1 promotes Flt 1 gene expression and induces mRNA expression of VEGF , Flk 1 , and Tie 2 , leading to enhancement of mature angiogenesis in vivo . ^^^ Thus , EPAS 1 may contribute to the construction of mature vessels by modulating the coordinated expressions of VEGF , Flt 1 , Flk 1 , and Tie2 . . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
To address angiogenesis of endochondral ossification in the condyle , we examined the appearance of vascular endothelial growth factor ( VEGF ) and its receptor Flt 1 in condylar cartilage of the growing rat . ^^^ From the findings of Flt 1 immunoreactivity , the VEGF produced by the chondrocytes of the hypertrophic zone should contribute to the promotion of endothelial cell proliferation and to stimulate migration and activation of osteoclasts in condylar cartilage , resulting in the invasion of these cells into the mineralized zone . . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
In this study , the expression of vascular endothelial growth factor ( VEGF ) , basic fibroblast growth factor ( bFGF ) , and VEGF receptors , fms like tyrosine kinase ( Flt 1 ) and kinase insert domain containing region ( KDR ) , and bFGF receptor 1 ( Flg ) were examined in the endometrium of rhesus monkey on Day 5 , 10 , 16 , 20 , 25 of menstrual cycle and on Day 19 of early pregnancy . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
We demonstrate here that human lymphoma cells secrete vascular endothelial growth factor ( VEGF ) and express VEGF receptor 1 ( VEGFR 1 ) and VEGFR 2 . ^^^ Proliferation of non Hodgkin lymphoma ( NHL ) cells under serum free conditions was enhanced by the addition of VEGF and was blocked by VEGFR 1 and VEGFR 2 specific antibodies . ^^^ To differentiate between VEGF mediated autocrine and paracrine effects on lymphoma growth , NOD / SCID mice engrafted with human diffuse large B cell lymphoma ( DLBCL ) were treated with species specific antibodies against human VEGFR 1 ( 6 . 12 ) , human VEGFR 2 ( IMC 1C11 ) , murine VEGFR 1 ( MF 1 ) , or murine VEGFR 2 ( DC 101 ) . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
OBJECTIVE : Soluble vascular endothelial growth factor receptor 1 ( sVEGFR 1 ) , which antagonizes VEGF functions , has been implicated in the pathophysiology of preeclampsia . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
We used old C57BL / 6J mice that are affected by rapid and severe age related hearing loss , and analyzed the expression pattern of vascular endothelial growth factor ( VEGF ) , a prototypical angiogenic cytokine , and its receptors Flt 1 and Flk 1 in the inner ear . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Biweekly treatment with vehicle or the VEGF Trap , a high affinity soluble decoy receptor incorporating regions of VEGFR 1 and VEGFR 2 , was begun 1 week later ( 100 or 500 micrograms / dose , n=20 in each group ) . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
The gene transfer of soluble VEGF type 1 receptor ( Flt 1 ) attenuates peritoneal fibrosis formation in mice but not soluble TGF beta type 2 receptor gene transfer . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
To investigate whether VEGF and VEGF receptor [ kinase insert domain containing receptor ( KDR ) and Flt 1 ] expression are lower with obesity , vastus lateralis muscle biopsies were obtained from eight obese and eight lean young sedentary men before and 2 h after a 1 h submaximal aerobic exercise bout for the measurement of VEGF , KDR , Flt 1 , and skeletal muscle fiber and capillary characteristics . ^^^ Exercise increased VEGF ( 10 fold ) , KDR ( 3 fold ) , and Flt 1 ( 5 fold ) mRNA independent of group . ^^^ There were no differences in VEGF , KDR , or Flt 1 protein between groups . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
During the first week of treatment , vascular endothelial growth factor ( VEGF ) , VEGF receptor 1 ( Flt 1 ) , and basic fibroblast growth factor proteins were higher in the treated group , whereas VEGF receptor 2 ( Flk 1 ) , angiopoietin 1 , and angiopoietin 2 were not affected by treatment . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
OBJECTIVE : To explore the expression of vascular endothelial growth factor ( VEGF ) and its receptors ( flt 1 and flk 1 ) in the retina of retinopathy of prematurity ( ROP ) , and its relation to the alteration of retinal blood vessels . ^^^ The expression of VEGF , flt 1 and flk 1 in the retina was measured by immunohistochemistry . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
VEGF binds VEGFR 1 as well , but the importance of VEGFR 1 signaling in vascular permeability has been largely neglected . ^^^ TfsvVEGF bound VEGFR 1 and induced its autophosphorylation to almost the same extent as VEGF ( 165 ) , but bound VEGFR 2 weakly and induced its autophosphorylation almost 10 fold less effectively than VEGF ( 165 ) . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
VEGF receptor , a naturally occurring soluble form of the VEGFR 1 ( sVEGFR 1 . flt 1 ) , is produced by endothelial cells by differential splicing of the flt 1 gene , and is a negative counterpart of the VEGF signaling pathway . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
METHODS : The tissue samples of 42 primary ovarian epithelial carcinoma , 29 benign ovarian tumor and 11 normal ovarian tissue were used to determine the expression of VEGF , VEGFR 1 , VEGFR 2 , P STAT 1 , P STAT 3 , P STAT 5 and P STAT 6 proteins by immunohistochemical staining . ^^^ However , in ovarian carcinoma cells , expressions of VEGF , VEGFR 1 and VEGFR 2 were significantly correlated with P STAT 3 and P STAT 5 , but not with P STAT 1 and P STAT 6 . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
METHODS : Rats were exposed to alternating cycles of high and low levels of oxygen for 14 days immediately after birth ; thereafter , they were intravitreally injected with an adenoviral vector expressing a secreted form of the VEGF receptor flt 1 ( Ad . sflt ) , which acts by sequestering VEGF . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Biological effects of VEGF are mediated by endothelial cell surface receptors , KDR and Flt 1 . ^^^ Alternative Flt 1 RNA processing , involving retention of intron 13 and the use of intronic cleavage polyadenylation signals , produces a secreted form of Flt 1 , `` sFlt 1 , ' ' that binds VEGF with high affinity and can inhibit VEGF signaling . ^^^ The reciprocity between sFlt 1 and Flt 1 mRNA expression suggests a novel post transcriptional mechanism by which sFlt 1 protein production and , thereby , responsiveness to VEGF , may be modulated . . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
However , the presence of FGF 2 in the medium up regulated the expression of both VEGF receptors ( VEGFR 1 and VEGFR 2 ) , whereas oestradiol or VEGF treatment showed no effect on the expression of these receptors . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Candidate cervical cancer specific TSPs include promoters of the genes for secretory leukoprotease inhibitor ( SLPI ) , cyclooxygenase 2 ( COX 2 ) , Midkine ( MK ) , vascular endothelial growth factor receptor type 1 ( flt 1 ) , vascular endothelial growth factor ( VEGF ) , Survivin and the receptor for chemokine SDS 1 ( CXCR 4 ) . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
OBJECTIVE : To compare the mRNA expression of vascular endothelial growth factor ( VEGF ) and its receptors ( KDR and flt 1 ) in the implantation and nonimplantation sites of the human oviduct with ectopic gestation . ^^^ RESULT ( S ) : The mRNA expression of VEGF and its receptors , both KDR and flt 1 , was significantly higher in the implantation site of the human oviduct with ectopic gestation compared with the nonimplantation site . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Transfection with the murine soluble Flt 1 ( sFlt 1 ) gene to block VEGF in vivo in mice inhibited early inflammation and later neointimal formation . ^^^ The sFlt 1 gene transfer did not affect plasma lipid levels but attenuated increased expression of VEGF , Flt 1 , Flk 1 , monocyte chemoattractant protein 1 , and other inflammation promoting factors . ^^^ CONCLUSIONS : Inflammatory changes mediated by VEGF and Flt 1 signals play an important role in the pathogenesis of neointimal formation after cuff induced periadventitial injury . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
METHODS AND RESULTS : C57BL / 6J mice ( n=28 ) were subjected to unilateral hindlimb ischemia after intravenous injection of recombinant adenoviruses ( 10 ( 9 ) plaque forming units ) encoding the ligand binding ectodomain of VEGF receptor 1 ( VEGFR1 / Ad Flt 1 ) , VEGF receptor 2 ( VEGFR2 / Ad Flk 1 Fc ) , a control murine IgG2alpha Fc fragment ( Ad Fc ) , or vehicle ( phosphate buffered saline ) . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Induction of VEGF and its Flt 1 receptor after sciatic nerve crush injury . ^^^ Immunohistochemical staining revealed strikingly different distribution of VEGF , Flt 1 , and Flk 1 in lumbar motor neurons . ^^^ VEGF was observed both in the nuclei and perikarya , while Flk 1 had cytoplasmic and Flt 1 perinuclear localization . ^^^ Real time RT PCR showed a significant increase in the expression of VEGF and Flt 1 on the injured side of the lumbar spinal cord . ^^^ Here we show that lumbar motor neurons increase the expression of VEGF and Flt 1 in response to injury . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
To address this , we explored the effect of oxygen tension on the expression of VEGF , placenta growth factor ( PlGF ) , and their antagonist , soluble fms like tyrosine kinase 1 ( sFlt 1 ) using ELISA and real time PCR in a primary CT cell culture . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Expression of vascular endothelial growth factor ( VEGF ) and its receptors ( VEGF R 1 [ Flt 1 ] and VEGF R 2 [ KDR / Flk 1 ] ) in tumorlets and in neuroendocrine cell hyperplasia of the lung . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
In a Matrigel plug assay , neither Ang 1 nor VEGF alone induced angiogenesis but together stimulated infiltration of non endothelial cells that were CD 31 negative , vimentin positive and also positive for VEGFR 1 and Tie 2 . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
VEGF signaling proceeds via autophosphorylation of VEGFR 1 and activation of the phosphatidylinositol 3 kinase ( PI3K ) pathway . ^^^ Thus , podocyte derived VEGF operates in an autocrine loop , likely through VEGFR 1 and PI3K , to stimulate alpha 3 ( 4 ) collagen production . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
The role of the vascular endothelial growth factors ( VEGF ) receptors ( KDR and Flt 1 ) and their characteristics in VEGF induced vasodilation in human vessels is unclear . ^^^ The cumulative concentration ( 12 to 8 log10M ) relaxation curves were established for VEGF , KDR SM , Flt 1 SM , and placenta growth factor ( PlGF ) in the absence or presence of indomethacin ( INDO , 7 microM ) , N nitro L arginine ( L NNA , 300 microM ) , L NNA + oxyhemoglobin ( HbO , 20 microM ) , or INDO + L NNA + HbO . ^^^ In the endothelium intact vessel rings , VEGF ( 63 . 2 + / 3 . 9 % ) induced significantly more ( P < 0 . 001 ) relaxation than Flt 1 SM ( 28 . 5 + / 4 . 3 % , 95 % CI 18 . 1 51 . 3 % ) , and PlGF ( 26 . 0 + / 4 . 7 % , 95 % CI 17 . 6 56 . 8 % ) . ^^^ The maximal relaxation induced by KDR SM ( 53 . 0 + / 4 . 0 % ) was only slightly less than that by VEGF ( P = 0 . 075 ) but significantly more than that by Flt 1 SM ( P = 0 . 001 , 95 % CI 7 . 8 41 . 1 % ) . ^^^ INDO + L NNA + HbO completely inhibited the relaxation by VEGF , KDR SM , or Flt 1 SM . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
The expression of both VEGF receptor 1 ( VEGFR 1 ) and VEGFR 2 was upregulated both in the satellite cells of the damaged muscles and during myotube formation in vitro ; the VEGF effect was mediated by the VEGFR 2 , since the transfer of PlGF , a VEGF family member interacting with the VEGFR 1 , was ineffective . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Our previous study found that a novel low molecular weight compound , VGA 1155 , inhibited binding between radioisotope labelled VEGF and cells overexpressing its two receptors , Flt 1 and KDR / Flk 1 , that is , NIH3T3 Flt 1 and NIH3T3 KDR , respectively . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Together with flt 1 ( VEGF receptor 1 ) and flk 1 ( VEGF receptor 2 ) , neuropilin ( NP ) is frequently described as being involved in the neuroprotective effects of VEGF . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
METHODS : Colonic specimens were taken from 42 patients with ulcerative colitis ( UC ) , 37 with Crohn ' s disease ( CD ) , 8 with non IBD colitis , and 21 normal controls . ( 1 ) Expression of vascular endothelial growth factor ( VEGF ) , VEGF receptors ( Flt 1 , KDR ) , and Ets 1 proteins in colonic mucosa was immunohistochemically examined using specific antibodies . ( 2 ) Expression of Ets 1 protein or VEGF , Flt 1 , KDR , and Ets 1 mRNA in colonic mucosa was measured by Western blot or RT PCR . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
This study was designed to test the hypothesis that ( a ) the cervix of pregnant rats expresses vascular endothelial growth factor ( VEGF ) and components of the angiogenic signaling pathway [ VEGF receptors ( Flt 1 , KDR ) , activity of protein kinase B , Akt ( phosphorylated Akt ) , and endothelial nitric oxide synthase ( eNOS ) ] and von Willebrand Factor ( vWF ) and that these molecules undergo changes with pregnancy , and ( b ) bilateral pelvic neurectomy ( BLPN ) alters levels of VEGF concentration in the cervix . ^^^ VEGF , VEGF receptor 1 ( Flt 1 ) , eNOS , and vWF immunoreactivities ( ir ) were localized in the microvasculature of cervical stroma . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
RESULTS : In 107 AML patients , the positive mRNA expression rate of VEGF , KDR and Flt 1 was 56 . 1 % , 49 . 5 % , and 2 . 8 % , respectively ; for bFGF and FGFR 1 4 , the positive rate was 21 . 5 % , 35 . 5 % , 9 . 4 % , 35 . 5 % and 23 . 4 % , respectively . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Compared with normal mice , 1 year old ApoE ( / ) mice fed on a high fat diet ( HFD ) had about 30 % less myocardial capillary ( P < 0 . 001 ) and arteriolar ( P < 0 . 03 ) densities , associated with decreased VEGF ( 55 % ) , VEGFR 1 ( 56 % ) and VEGFR 2 ( 78 % ) mRNA expressions and myocardial endothelial nitric oxide synthase ( eNOS ) production ( 58 % ) . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
METHODS : Ovine left ( LV ) and right ventricle ( RV ) samples were obtained at four time points : 95 days ' and 140 days ' gestation ( term = 145 days ) and 7 days and 8 weeks postnatally . mRNA and protein levels of vascular endothelial growth factor ( VEGF ) , its respective receptors ( Flk 1 and Flt 1 ) , basic fibroblast growth factor ( bFGF ) , transforming growth factor beta 1 ( TGF beta 1 ) , and endothelial nitric oxide synthase ( eNOS ) were measured at these different time points . ^^^ CONCLUSION : Developmentally regulated ventricle specific expression of VEGF , Flt 1 , Flk 1 , TGF beta 1 , bFGF , and eNOS was demonstrated in the ovine myocardium . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
To evaluate a possible contribution of the VEGF receptors , we performed immunohistochemical stainings of VEGF R 1 ( flt ) , R 2 ( KDR , flk ) , and Neuropilin 1 . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
EXPERIMENTAL DESIGN : Real time quantitative reverse transcriptase PCR was used to measure levels of mRNA of tumor angiogenesis and lymphangiogenesis related factors [ vascular endothelial growth factor ( VEGF ) A , VEGF C , VEGF D , Flt 1 , KDR , Flt 4 , Ang 1 , Ang 2 , Tie 1 , Tie 2 , cyclooxygenase 2 , fibroblast growth factor 2 ( FGF 2 ) , Egr 1 , Prox 1 , and LYVE 1 ] in tumor specimens of 16 inflammatory breast cancer and 20 noninflammatory breast cancer patients . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Urinary gonadotrophins but not recombinant gonadotrophins reduce expression of VEGF 120 and its receptors flt 1 and flk 1 in the mouse uterus during the peri implantation period . ^^^ RESULTS : Urinary gonadotrophin treatment caused lower levels of VEGF 120 , flt 1 and flk 1 mRNA levels , reduced the size of the embryo implantation site , delayed implantation and prolonged the gestational period . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Vascular endothelial growth factor ( VEGF ) regulates vasculogenesis and angiogenesis by using two tyrosine kinase receptors , VEGFR 1 and VEGFR 2 . ^^^ In VEGFR 1 ( TM TK ) ( / ) mice with growth arrest , membrane targeted VEGF was reduced , resulting in the suppression of VEGFR 2 phosphorylation . ^^^ These results strongly suggest that the membrane fixed ligand binding region of VEGFR 1 traps VEGF for the appropriate regulation of VEGF signaling in vascular endothelial cells during early embryogenesis . . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Immunohistochemical staining for vascular endothelial growth factor ( VEGF ) , its receptors Flk 1 and Flt 1 , ephrin B 2 , MIB 1 and proliferating cell nuclear antigen ( PCNA ) was performed using standard immunohistochemical techniques . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Animal models with mutations in the genes coding for placental growth factor ( PlGF ) , a member of vascular endothelial growth factor ( VEGF ) family , or the tyrosine kinase domain of the PlGF receptor ( Flt 1 ) have revealed differences between normal physiological angiogenesis and pathological angiogenesis associated with conditions such as tumor growth , arthritis and atherosclerosis . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Exposure of wild type , VEGFR 1 ( / ) , or VEGFR 2 ( / ) embryonic stem cells to exogenous VEGF or the VEGFR 1 specific ligand , placental growth factor , revealed distinct roles of VEGF receptors . ^^^ VEGFR 1 is the primary mediator of the VEGF inhibition of DC maturation , whereas VEGFR 2 tyrosine kinase signaling is essential for early hemopoietic differentiation , but only marginally affects final DC maturation . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Although much is known about the biology of vascular endothelial growth factor ( VEGF ) and its cognate receptors ( VEGFRs ) , VEGFR 1 and VEGFR 2 , little is known about the roles of the VEGFRs neuropilin ( NP ) 1 and NP 2 in the primate endometrium . ^^^ A recent study showed that blockade of VEGF action can inhibit luminal epithelial cell proliferation , but there is no evidence of VEGFR 1 and VEGFR 2 expression in these cells . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
The expression of fms like tyrosine kinase ( Flt ) 1 , a vascular endothelial growth factor ( VEGF ) receptor , was enhanced in AMF expressing tumors dependent on protein kinase C and phosphatidylinositol 3 kinase ( PI3K ) activation ; meanwhile kinase insert domain containing receptor , another receptor of VEGF , was not . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
VEGF activity is mediated by two receptors , VEGFR 1 ( Flt 1 ) and VEGFR 2 ( Flk 1 / KDR ) , which are expressed primarily in vascular endothelial cells . ^^^ Reduced cycle , specific primer , reverse transcriptase polymerase chain reaction was performed to determine the expression of VEGF and its receptors , VEGFR 1 and VEGFR 2 . ^^^ VEGF and its receptors , VEGFR 1 and VEGFR 2 , increase expression during skin development and dermal differentiation . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
ROP eyes were further analyzed for changes in VEGF and VEGF receptor ( Flt 1 and Flk 1 ) protein content following rapamycin treatment . ^^^ Quantitative analysis of total protein via enzyme linked immunosorbent assay ( ELISA ) confirmed an increase in Flt 1 and VEGF , following drug treatment , with no effect on Flk 1 . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
C / CR gene expression ( interleukin [ IL ] 7 , IL 10 , IL 12p40 , IL 15 , IL 18 , IL 7Ralpha , IL 10R1 , IL 15Ralpha , interferon gamma [ IFN gamma ] , vascular epithelial growth factor [ VEGF ] , Flt 1 , and transforming growth factor beta ) was quantified by real time reverse transcriptase polymerase chain reaction and correlated with protein expression by immunofluorescence . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
We examined the radiological and histological features of , and the influences of the expression of VEGF and its two major receptors , Flt 1 and Flk 1 , on the development of peritumoral brain edema ( PTBE ) in patients with intracranial meningiomas . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
The discovery of the vascular endothelial growth factor ( VEGF ) family members VEGF , VEGF B , placental growth factor ( PlGF ) , VEGF C and VEGF D and their receptors VEGFR 1 , 2 and 3 has provided tools for studying the vascular system in development as well as in diseases ranging from ischemic heart disease to cancer . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Differential gene expression analysis using DNA microarrays revealed that overexpression of STAT3beta down regulates the VEGF receptors Flt 1 , neuropilins 1 and 2 , and the inhibitor of DNA binding / differentiation ( Id 2 ) gene product involved in the neoplastic transformation . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Concentrations of VEGF and VEGFR 1 in paired tumor arteries and veins in patients with rectal cancer . ^^^ The aim was to investigate the contribution of the tumor to plasma concentrations of VEGF and VEGF receptor 1 ( VEGFR 1 ) . ^^^ Plasma concentrations of VEGF and VEGFR 1 were determined by ELISA . ^^^ There was a significant efflux of neutrophils to the tumor , but none of the observed changes in plasma VEGF or VEGFR 1 levels correlated to changes in counts of white blood cells or platelets ( sVEGF : 0 . 33 < P < 0 . 73 and sVEGFR 1 : 0 . 32 < P < 0 . 98 ) . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Association between intratumoral free and total VEGF , soluble VEGFR 1 , VEGFR 2 and prognosis in breast cancer . ^^^ Vascular endothelial growth factor ( VEGF ) receptors consist of three cell membrane type receptors ( VEGFR 1 , VEGFR 2 and VEGFR 3 ) , and soluble form of VEGFR 1 ( sVEGFR 1 ) , an intrinsic negative counterpart of the VEGF . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Vascular endothelial growth factor ( VEGF ) and its receptors VEGFR 1 ( Flt 1 ) and VEGFR 2 ( KDR / Flk 1 ) could play a role in tissue remodelling and angiogenesis in COPD . ^^^ METHODS : The cellular expression pattern of VEGF , Flt 1 , and KDR / Flk 1 was examined by immunohistochemistry in central and peripheral lung tissues obtained from ex smokers with COPD ( forced expiratory volume in 1 second ( FEV ( 1 ) ) < 75 % predicted ; n = 14 ) or without COPD ( FEV ( 1 ) > 85 % predicted ; n = 14 ) . ^^^ RESULTS : VEGF , Flt 1 , and KDR / Flk 1 immunostaining was localised in vascular and airway smooth muscle ( VSM and ASM ) cells , bronchial , bronchiolar and alveolar epithelium , and macrophages . ^^^ Pulmonary endothelial cells expressed Flt 1 and KDR / Flk 1 abundantly but not VEGF . ^^^ CONCLUSIONS : VEGF and its receptors Flt 1 and KDR / Flk 1 may be involved in peripheral vascular and airway remodelling processes in an autocrine and / or paracrine manner . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
The actions of VEGF are mediated through receptors possessing tyrosine kinase activity : VEGFR 1 ( Flt 1 ) , VEGFR 2 ( Kdr / Flk 1 ) and VEGFR 3 ( Flt 4 ) . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Additionally , expression of transforming growth factor beta ( TGF beta ) , vascular endothelial growth factor ( VEGF ) , flt 1 , endothelial nitric oxide synthase ( eNOS ) , endothelin 1 ( ET 1 ) , endothelin A receptor ( ETA ) receptor , and alpha estrogen receptor was assessed using immunohistochemistry . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
To determine the significance of VEGF Flt 1 ( VEGFR 1 ) signaling in ischemic preconditioned ( PC ) myocardium , we used heterozygous Flt 1 knockout ( KO ) mice to dissect the pathway and identify candidate genes involved in VEGF signaling . ^^^ This study attempts to address an important clinical issue by identifying potential candidates of VEGF signaling through Flt 1 receptor that trigger angiogenic signal under ischemic stress . ^^^ This observation may be due to downregulation of several important genes such as growth regulated oncogene 1 ( Gro 1 ) , heat shock proteins ( HSP ) , 1 kappa B kinase beta ( IKK beta ) , colony stimulating factor 1 ( CSF 1 ) and annexin A 7 , suggesting the importance of VEGF Flt 1 receptor signaling during PC . . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
The present objectives were to determine the concentration of VEGF and the high affinity receptor VEGFR 1 in VS homogenates and to examine a possible correlation with symptom duration , tumor size , or growth rate . ^^^ ELISA was used for determination of the VEGF and VEGFR 1 concentration in tumor homogenates . ^^^ RESULTS : All tumor homogenates contained VEGF and VEGFR 1 . ^^^ A significant correlation existed between the concentration of both VEGF and VEGFR 1 and tumor growth rate but not symptom duration or tumor size . ^^^ CONCLUSION : The concentration of VEGF and VEGFR 1 in VS homogenates correlates with tumor growth rate but not with tumor size or symptom duration . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Protein expression of the most potent angiogenic factor , vascular endothelial growth factor ( VEGF ) , and its specific natural inhibitor , soluble Flt 1 , as well as vessel architecture , including vessel density , area , and diameter , was evaluated in human malignant glioma samples ( 24 glioblastomas , 13 anaplastic astrocytomas ) . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
BACKGROUND AND AIMS : Recombinant erythropoietin upregulates the expression of the vascular endothelial growth factor ( VEGF ) receptors , Flt 1 ( VEGFR 1 ) and KDR / Flk 1 ( VEGFR 2 ) , in endothelial cells . ^^^ As albuminuria / proteinuria is a hallmark of diabetic nephropathy , we examined cross sectionally in 35 type 1 and 37 type 2 diabetic patients with various degrees of renal dysfunction and albuminuria whether there was an interrelationship between intrinsic erythropoietin ( EPO ) and VEGF / Flt 1 . ^^^ No correlation between VEGF or Flt 1 and EPO was apparent . ^^^ CONCLUSION : Our data suggest that in vivo EPO does not affect the functionality and / or production of components of the VEGF / Flt 1 system in diabetics with normal or reduced renal function . . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Messenger RNA expressions of Flt 1 , VEGF and PlGF were determined by RT PCR . ^^^ The increased mRNA expressions for Flt 1 and VEGF and the decreased mRNA expression for PlGF in TCs were consistent with the protein productions under hypoxia condition . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Finally , we showed that VEGF induced TAL 1 expression in the NGM which resulted in the formation of ectopic structures was mediated by Flk 1 but not Flt 1 signaling . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
The mRNA for angiogenesis related factors , including hypoxia inducible factor 1alpha ( HIF 1alpha ) , vascular endothelial growth factor ( VEGF ) , kinase insert domain containing receptor / fetal liver kinase 1 ( KDR / Flk 1 ) , fms like tyrosine kinase ( Flt 1 ) , angiopoietin 1 and tyrosine kinase with Ig and epidermal growth factor ( EGF ) homology domain 2 ( Tie 2 ) , was analysed using a semi quantitative reverse transcriptase polymerase chain reaction ( RT PCR ) . ^^^ These findings indicate that reduction in the expression of mRNAs in the VEGF / KDR / Flk 1 and Flt 1 as well as angiopoietin 1 / Tie 2 signal pathways might be one of the reasons for the capillary regression during chronic denervation . . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Relative to human VEGF 165 , the binding affinity of Pm VEGF to the VEGFR 1 was 1 . 7 fold higher while affinity to the VEGFR 2 was 17 fold lower . ^^^ Structural comparison among VEGF proteins from various viper venoms revealed that the two subfamilies of vipers ( Crotalinae and Viperinae ) have evolved with distinct receptor specificities for VEGFR 1 and VEGFR 2 , respectively . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
To determine the individual roles of VEGF receptors ( VEGFR ) 2 and 1 on postnatal lung growth , neonatal mice were treated with neutralizing antibodies to VEGFR 2 ( DC 101 ) or VEGFR 1 ( MF 1 ) in the perinatal period . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
AIM : To observe the effect of Chinese traditional herbal decoction Weikang ning ( WKN ) on cell growth and expression of VEGF and its receptors KDR and Flt 1 in gastric cancer cell line MGC 803 . ^^^ The expression of mRNA of VEGF and its receptors KDR and Flt 1 was detected with RT PCR . ^^^ CONCLUSION : The decoction of WKN suppresses the growth of gastric cancer cell MGC 803 and decreases the expression of mRNA of both VEGF and its receptors KDR and Flt 1 . . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Vascular endothelial growth factor ( VEGF ) , its receptors ( VEGFR 1 , VEGFR 2 ) and neuropillin 1 ( NRP 1 ) are expressed at variable levels in bone marrow . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
The aim of this study was to investigate the prognostic value of vascular endothelial growth factor ( VEGF ) receptors , VEGFR 1 ( Flt 1 ) and VEGFR 2 ( KDR / Flk 1 ) , and Tie2 / tek receptor tyrosine kinase in breast carcinoma . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Trx overexpressed HT 1080 cells induced migration of endothelial cells through Flt 1 vascular endothelial growth factor ( VEGF ) receptor type 1 . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
CONCLUSION : Serum VEGF and VEGFR 1 might be useful markers for gauging the clinical effect of various treatments on patients . . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
The non angiogenic role of vascular endothelial growth factor ( VEGF ) , and its receptors flt 1 and flk 1 , together with downstream signaling pathways were examined in fetal and postnatal rat cerebral cortical organotypic explants . ^^^ The VEGF receptor flt 1 was observed on most , though not all astrocytes , while flk 1 receptor immunoexpression was absent . ^^^ Treatment with antisense oligonucleotides ( AS ODNs ) to flt 1 resulted in a dramatic decrease in GFAP and nestin immunoreactivity , which further confirmed the role of flt 1 in mediating VEGF ' s gliotrophic effects , while AS ODNs to flk 1 had no effect . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
A longer unique antisense oligo against a different region of pICln mRNA was found to also enhance EC growth and tube formation and to decrease mRNAs for soluble Flt 1 and neuropilin 1 vascular endothelial growth factor ( VEGF ) receptors , the angiostatic factors that are generated by alternative RNA splicing . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
In addition , 11 , 11 ' dideoxyverticillin decreased VEGF secretion by MDA MB 468 breast cancer cells , and significantly suppressed VEGF induced tyrosine phosphorylation of Flt 1 and KDR / Flk 1 . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
LSAB immunohistochemical staining was used to determine the expression of VEGF , VEGFR 1 , VEGFR 2 and activated STATS ( P STAT 1 , P STAT 3 , P STAT 5 , P STAT 6 ) proteins . ^^^ In cancer cells , VEGF , VEGFR 1 and VEGFR 2 were all significantly correlated with P STAT 3 and P STAT 5 ( P = 0 . 000 ) , but not with P STAT 1 or P STAT 6 . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Expression of VEGF and the VEGF receptors VEGFR 1 ( Flt 1 ) and VEGFR 2 ( Flk 1 ) were studied by in situ hybridization and immunohistochemistry . ^^^ It was also noted that Flt 1 / Flk 1 and VEGF positive vessels often were negative for SMI 71 , a marker for vessels in areas with blood brain barrier ( BBB ) . ^^^ In conclusion , we have demonstrated that TBI leads to an upregulation of VEGF , Flt 1 , and Flk 1 mRNA and protein in and around the lesion . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Soluble FLT 1 suppressed these activities in TCL 1 cells , indicating that signals involving VEGF axis are essential for endovascular differentiation . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
The inhibition of FGFR 3 expression by ribozymes was associated with decreased vascular endothelial growth factor ( VEGF ) expression and upregulation of Flt 1 but not of the KDR receptor . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Our data lend support to the hypothesis that VEGF is essential for pregnancy establishment and that trophoblast derived VEGF , acting via its specific receptors Flt 1 and KDR , is necessary for blastocyst implantation . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
The expressions of VEGF and its functional receptor , fms like tyrosine kinase ( Flt ) 1 , were quantified in AMs by real time RT PCR and , further , the level of VEGF in BAL fluid was determined by ELISA . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Recently , the soluble form of VEGFR 1 ( sVEGFR 1 ) , an antagonist to VEGF and PIGF , has been implicated in the pathophysiology of pre eclampsia . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
The expression of P 53 , VEGF , Flt decreased with the dosage increasing of JPDZF . ^^^ CONCLUSION : Traaditional Chinese medicine JPDZF can inhibit the growth of gastric carcinoma cells and the expression of P 53 , VEGF , Flt . . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Vascular endothelial growth factor receptor 1 ( VEGFR 1 ) and receptor 2 ( VEGFR 2 ) , are the high affinity VEGF receptors , which play an important role in de novo blood vessel formation and hematopoietic cell development . ^^^ Vascular endothelial growth factor receptor 1 ( VEGFR 1 ) and receptor 2 ( VEGFR 2 ) , are the high affinity VEGF receptors , which play an important role in de novo blood vessel formation and hematopoietic cell development . ^^^ The aim of our study was to compare the concentration of VEGF , VEGFR 1 and VEGFR 2 in the serum of 83 , never treated B CLL patients in different stage of disease according to Rai classification , and 20 healthy volunteers . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
It has been shown that the interaction between the potent angiogenic factor ; the vascular endothelial growth factor ( VEGF ) and its receptors ( VEGFR 1 and VEGFR 2 ) , plays a pivotal role in tumor development , including hepatocellular carcinoma ( HCC ) . ^^^ We examined the signaling cascades of VEGFR 1 and VEGFR 2 in the VEGF mediated HCC development in combination with a retroviral tetracycline ( tet ) regulated ( Retro Tet ) gene expression system , which can manipulate the gene expression in vivo by providing tet in the drinking water , as well as VEGFR 1 and VEGFR 2 specific neutralizing monoclonal antibodies ( R 1mAb and R 2mAb , respectively ) . ^^^ These results suggested that both VEGFR 1 and VEGFR 2 play important roles , and lie in the different signaling cascades by which VEGF augments HCC development and angiogenesis . . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Breast cancer metastases to bone were immunohistochemically stained for VEGF , its receptors VEGFR 1 and 2 ( vascular endothelial growth factor receptor 1 and 2 ) , demonstrating that breast cancer metastases express VEGF strongly and that surrounding osteoclasts express both VEGFR 1 and VEGFR 2 . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
PURPOSE : The purpose of this study was to develop antagonists specific for the vascular endothelial growth factor receptor 1 ( VEGFR 1 ) and to investigate the effects of the antagonists on the VEGF induced endothelial cell functions and tumor progression . ^^^ EXPERIMENTAL DESIGN : Hexapeptides that inhibit binding of VEGFR 1 and VEGF were identified through screening of synthetic peptide library . ^^^ RESULTS : Here , we report on a short peptide that binds to VEGFR 1 and prevents binding of VEGF . ^^^ The anti Flt 1 peptide shows specificity toward binding to VEGFR 1 and it inhibits binding of VEGF , placental growth factor ( PlGF ) , and VEGF / PlGF heterodimer to VEGFR 1 . ^^^ Accordingly , VEGF induced migration and capillary formation are mediated through VEGFR 1 , and these processes may play an important role in the growth and metastasis of VEGF secreting tumors . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
In thyroids from three RAd treated animals , there was marked , significant inhibition of Tie 2 , FGFR 1 , VEGFR 1 , FGF 2 , and VEGF expression , compared with control goiters . ^^^ VEGF and VEGFR 1 expression was reduced in these thyroids . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
This vascular effect follows binding to two receptors , VEGF receptor 1 ( VEGFR 1 ) and VEGF receptor 2 ( VEGFR 2 ) , the latter of which is thought to be the main receptor responsible for the vasorelaxing effect of VEGF . ^^^ VEGF vasorelaxation may not only occur through binding to VEGFR 2 , since PlGF , the specific VEGFR 1 agonist , also causes vasodilation . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
By immunohistochemical staining , VEGF was not detected in normal uterine smooth muscle , but VEGF receptor 1 ( flt 1 ) and VEGF receptor 2 ( flk 1 ) were observed in 14 and 4 of 14 normal smooth muscles , respectively . ^^^ Of 39 sarcomas , 25 expressed VEGF , and 38 and 34 sarcomas expressed flt 1 and flk 1 at various intensities , respectively . ^^^ The staining intensity of VEGF , flt 1 , and flk 1 was significantly higher in sarcoma than in normal uterine smooth muscle , but that of phospho flt 1 ( p flt 1 ) was significantly lower in sarcoma than in normal uterine smooth muscle . ^^^ In conclusion , VEGF and its receptors are suggested to be involved in progression of uterine sarcoma , but only the p flt 1 level significantly affected the survival of leiomyosarcoma patients . . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
We studied protein expression of basic fibroblast growth factor ( bFGF ) , vascular endothelial growth factor ( VEGF ) A , endothelin ( ET ) 1 , and VEGF C , and their receptors bFGF R 1 , Flt 1 , KDR , ET ( A ) R , ET ( B ) R , and Flt 4 immunohistochemically in 200 DCIS ( pure DCIS : n=96 ; DCIS adjacent to an invasive component : n=104 ) using self constructed tissue microarrays . ^^^ Basic fibroblast growth factor R 1 , VEGF C , Flt 4 , and ET ( A ) R were expressed in the tumour cells in the majority of cases , whereas bFGF and Flt 1 expression was rarely observed . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
The EMT also induces autocrine signaling involving VEGF and Flt 1 that enable invasive cells to become ' self sufficient ' for survival . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Flt 1 intraceptors inhibit hypoxia induced VEGF expression in vitro and corneal neovascularization in vivo . ^^^ METHODS : Human corneal epithelial cells , transfected with plasmids encoding Flt23K or Flt24K ( the VEGF binding domains of the Flt 1 receptor coupled with the endoplasmic reticulum retention peptide KDEL ) , were subjected 2 days after transfection to 5 % hypoxia for 24 hours . ^^^ RESULTS : Hypoxia induced VEGF by human corneal epithelial cells was sequestered by both Flt23K and Flt24K ; Flt 1 23K suppressed VEGF secretion as well . ^^^ CONCLUSIONS : Flt 1 intraceptors , which are endoplasmic reticulum retention signal coupled VEGF receptors , significantly suppress hypoxia induced VEGF secretion by corneal epithelial cells in vitro . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Surgical specimens and tumor cells in primary culture of salivary pleomorphic adenomas were used for immunohistochemistry for CD 31 , vascular endothelial growth factor ( VEGF ) and its receptors Flk 1 and Flt 1 , as well as for hypoxia markers , such as hypoxia inducible factor 1alpha ( HIF 1alpha ) and lactate dehydrogenase 1 ( LDH ) . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
This increase was inhibited by the VEGF receptor 2 inhibitor ZD 6474 as well as the VEGF inhibitor soluble Flt 1 . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
VEGF and its receptors ( flt 1 and KDR / flk 1 ) as prognostic indicators in endometrial carcinoma . ^^^ We investigated the expression and clinical significance of VEGF and its receptors , flt 1 and KDR / flk 1 , in patients with uterine endometrial carcinoma . ^^^ In endometrioid endometrial carcinomas , the positive immunostaining rate was 39 % for VEGF , 65 % for flt 1 and 68 % for KDR / flk 1 . ^^^ VEGF , flt 1 and KDR / flk 1 positive immunostainings were not related to poor prognosis . ^^^ We conclude that VEGF , flt 1 and KDR / flk 1 expressions are not useful prognostic markers for overall survival in patients with endometrioid endometrial carcinoma . . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Although mRNA for VEGF 121 , VEGF 165 , VEGFR 1 and NRP 2 was widely expressed , mRNA expression for VEGF 189 , VEGFR 2 and NRP 1 was linked to a malignant phenotype such as local invasion . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
OBJECTIVE : To study the distribution of isoforms of vascular endothelial growth factor ( VEGF ) and its receptors VEGFR 1 and VEGFR 2 in pterygia and to compare it with that in healthy conjunctivas . ^^^ MAIN OUTCOME PARAMETERS : Vascular endothelial growth factor and VEGFR 1 and VEGFR 2 were analyzed to indentify the splice variants of VEGF as well as the distribution and amount of VEGF and both receptors in pterygia and the control tissues . ^^^ In addition to VEGF , VEGFR 1 and VEGFR 2 were detected in pterygia and conjunctivas and immunostained within the epithelium of pterygia and conjunctivas and on intrapterygial and intraconjunctival endothelial cells . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
It is shown that bevacizumab potently neutralizes VEGF and blocks its signal transduction through both the VEGFR 1 and VEGFR 2 receptors , as demonstrated by the inhibition of VEGF induced cell proliferation , survival , permeability , nitric oxide production , as well as migration and tissue factor production . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
The mRNA species analyzed include VEGF and its receptors , VEGFR 1 and VEGFR 2 , and ANG 1 , ANG 2 and their receptor , Tie 2 . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
After induction of follicular growth by the eCG treatment , each mRNA expression of VEGF 120 , VEGF 164 , Neuropilin 1 and Flt 1 significantly increased . ^^^ Our findings suggest that ovarian vascular formation during the periovulatory period including preovulatory follicles , ovulation and CL formation may develop via crosstalk of the VEGF Flt 1 and Ang Tie 2 systems . . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
INTRODUCTION : Vascular endothelial growth factor ( VEGF ) and its receptors VEGFR 1 and 2 are considered to play a major in tumor angiogenesis , which is a prerequisite for growth of solid tumors . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
AIM : To determine plasma levels of angiopoietin 1 and angiopoietin 2 ( Ang 1 , Ang 2 ) , their soluble receptor Tie 2 , vascular endothelial growth factor ( VEGF ) , its soluble receptor Flt 1 ( as indices of angiogenesis ) , and von Willebrand factor ( vWf , marking endothelial damage / dysfunction ) in sickle cell disease ( SCD ) patients with proliferative sickle retinopathy ( PSR ) , with non proliferative retinopathy ( NPR ) , or no retinopathy ( NR ) and in control subjects with normal haemoglobin ( AA subjects ) . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
It has been shown that a soluble fragment of VEGF receptor Flt 1 ( sFlt 1 ) has anti angiogenic properties by way of its antagonist activity against VEGF . ^^^ The complex of sFlt 1 gene with PEI g PEG RGD conjugate efficiently inhibited the proliferation of cultured endothelial cells , representing that expressed sFlt 1 predominantly bound to exogenous VEGF and blocked the binding of VEGF to the full length Flt 1 receptor . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Vascular endothelial growth factor ( VEGF ) was labeled with radioiodine and evaluated in vitro as well as in vivo , using A 2058 , a melanoma cell line overexpressing VEGFR 1 and 2 . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
There are nine proteins in the immediate VEGF family : VEGFA , VEGFB , VEGFC , VEGFD , VEGF 3 , placental growth factor ( PGF ) , VEGF receptor ( VEGFR ) 1 , VEGFR 2 , and VEGFR 1 related . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Notably , APLD has been shown to have antiangiogenic properties which include : inhibition of VEGF secretion , reduction in the synthesis of the VEGF receptor ( FLT 1 ) , and blockade of matrix metalloproteinase production by endothelial cells . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Furthermore , by using DCs derived from Id 1 ( / ) mice that are defective in Flt 1 signaling , we demonstrated that the inhibitory function of VEGF on DC function is most likely mediated by Flt 1 . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
VEGF receptor 2 ( VEGFR 2 ) is mainly responsible for the dilator response to VEGF through nitric oxide ( NO ) release , whereas VEGFR 1 may sequestrate the ligand . ^^^ We speculate that sequestration by VEGFR 1 may also limit , to some extent , the vascular protecting effect of VEGF , thus contributing to the pathophysiologic changes seen in neonatal hypoxia induced pulmonary hypertension . . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Furthermore , VEGF receptor fms like tyrosine kinase 1 ( Flt 1 ) was elevated , whereas kinase insert domain containing receptor / fetal liver kinase 1 ( KDR ) was diminished in endothelial , but not epithelial , cells . ^^^ Like VEGF , FG 4095 and DMOG increased angiogenesis in vitro , both in 95 % and 21 % O 2 , an effect that could be blocked through either Flt 1 or KDR . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
At the early stage ( day 6 ) , glomerular expression of VEGF and receptors flk 1 and flt 1 as well as Ang 1 , and receptor Tie 2 were increased , and glomerular monocyte infiltration and the expression of angiopoietin 2 ( Ang 2 ) , a natural antagonist of Ang 1 , were reduced . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Soluble vascular endothelial growth factor receptors 1 , 2 ( Flt 1 , KDR ) are the negative counterpoint to the vascular endothelial growth factor ( VEGF ) signaling pathway , which has been characterized as one of the most important endothelial regulator in human angiogenesis . ^^^ In the present work , we tested the differential prognostic relevance of soluble vascular endothelial growth factor ( VEGF ) , their receptors 1 ( Flt 1 ) , 2 ( KDR ) , and the ratio between sVEGF / sFlt 1 in 43 patients with acute myeloid leukemia ( AML ) . sVEGF and its soluble receptors were assessed using an ELISA . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
RESULTS : There were no significant differences in the mRNA expression of VEGF and its receptors , both KDR and flt 1 , between the five study groups and the control group . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
We have shown here that adeno associated virus ( AAV ) mediated expression of sFlt 1 , a soluble form of the Flt 1 VEGF receptor , was maintained for up to 8 and 17 months postinjection in mice and in monkeys , respectively . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
We now report that normal human melanocytes ( Mc ) maintained in serum free , hormone , and growth factor supplemented medium lacking phorbol ester and choleragen constitutively express VEGF receptor 1 ( VEGFR 1 ) , VEGFR 2 , and neuropilin 1 . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
This current opinion will focus on the temporal and spatial expression of VEGF and its receptors VEGFR 1 and VEGFR 2 , and the angiopoietin receptors Tie 1 and Tie 2 in parallel to vascular maturation in human placental villi during very early stages of placental development . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Soluble VEGFR 1 binds to VEGF with a high affinity and therefore works to modulate VEGF and VEGF signaling pathway . ^^^ Differential splicing of the flt 1 mRNA generates soluble variant of vascular endothelial growth factor ( VEGF ) receptor 1 ( sVEGFR 1 , also known as sFlt 1 ) . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Here , we tested a recombinant adenovirus , AdsFlt 1 3 , that encodes an antagonistically acting fragment of the VEGF receptor 1 ( Flt 1 ) , for systemic antitumor effects in pre established subcutaneous CRC tumors in mice . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
OBJECTIVE : To examine the expression of vascular endothelial growth factor ( VEGF ) and its receptors ( VEGFR 1 , VEGFR 2 ) in transdifferentiated human proximal tubular epithelial ( HK 2 ) cell induced by transforming growth factor beta 1 ( TGFbeta 1 ) . ^^^ The cellular VEGF , VEGFR 1 , and VEGFR 2 were measured with Western blot . ^^^ CONCLUSIONS : Increased expression of VEGFR 1 and VEGFR 2 and two phase change in VEGF expression occurred in the process of tubular epithelial transdifferentiation induced by TGFbeta 1 . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Semi quantitative RT PCR analysis showed that VEGF receptor Flk 1 mRNA was also time dependently upregulated in the deafferented hippocampus with its maximal elevation at 7 15 days postlesion while the Flt 1 mRNA levels remained unchanged at any time point we examined . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
However , transcripts for VEGF 189 , VEGF 165 , and VEGF receptors ( Fms like tyrosine kinase 1 [ Flt 1 ] , kinase insert domain receptor [ KDR ] / fetal liver kinase 1 [ Flk 1 ] , and neuropilin 1 ) were suppressed in the BPD models . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
OBJECTIVE : To investigate the relationship of neonatal platelet count and preeclampsia to levels of soluble fms like tyrosine kinase 1 ( sFlt 1 ) , placental growth factor ( PlGF ) , and vascular endothelial growth factor ( VEGF ) in the cord blood of preterm infants . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
The VEGF receptors VEGFR 1 ( flt 1 ) and VEGFR 2 ( KDR ) , typically present on endothelial cells , have also been identified in human glioblastoma tissues and cell lines . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Bacterial antigen induced release of soluble vascular endothelial growth factor ( VEGF ) and VEGFR 1 before and after surgery . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Flt 1 , an `` fms like tyrosine kinase ' ' receptor , has been suggested to play an active role in vascular endothelial growth factor ( VEGF ) mediated autocrine signaling of tumor growth and angiogenesis . ^^^ Treatment with anti VEGF and anti Flt 1 antibodies inhibited ERK1 / 2 activation , down regulated bcl 2 , and reversed the hypoxia mediated drug resistance to etoposide . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
VEGF and its receptors , VEGFR 1 and VEGFR 2 , have been found to be expressed on subsets of normal and malignant hemopoietic cells , but the role of the individual receptors in hemopoiesis requires further study . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Vascular endothelial growth factor ( VEGF ) is a potent signalling molecule that acts through two tyrosine kinase receptors , VEGFR 1 and VEGFR 2 . ^^^ Exogenous VEGF addition resulted in extracellular signal regulated kinase 1 / 2 phosphorylation and cell proliferation , and both were reduced by VEGFR 1 blockade . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Plasma levels of vascular endothelial growth factor ( VEGF ) , basic fibroblast growth factor ( bFGF ) , interleukin 1 receptor alpha ( IL 1Ralpha ) , IL 6 , IL 8 , VEGF receptors VEGFR 1 and VEGFR 2 , and thrombopoietin ( TPO ) were measured in plasma samples of 95 patients by enzyme linked immunosorbent assay ( ELISA ) . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Vascular endothelial growth factor and VEGF receptors ( flt 1 and flk 1 ) were evaluated by Western blot , reverse transcriptase polymerase chain reaction ( RT PCR ) and real time RT PCR . ^^^ Remarkably , in diabetic patients , VEGF mRNA and protein levels were significantly higher , whereas flt 1 , flk 1 mRNA , and protein were lower when compared with non diabetic patients . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Treatment with vascular endothelial cell growth factor ( VEGF ) gave rise to endothelial like cells , expressing Flt 1 , Flk 1 , von Willebrand Factor ( vWF ) , CD 31 , acetylated low density lipoprotein internalization , and the ability to form endothelial like cell chains . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
OBJECTIVE : This study was undertaken to analyze prospectively circulating vascular endothelial growth factor ( VEGF ) and its soluble receptor , ( s ) Flt 1 , throughout normotensive and preeclamptic pregnancies and to assess the importance of these proteins in the development of preeclampsia . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
To assess antiangiogenesis therapy , tumor cells were administered 1 d after pneumonectomy and 1 d later , 5 10 10 ( 8 ) plaque forming units of Adsflt ( an Ad vector expressing the extracellular portion of the flt 1 vascular endothelial growth factor [ VEGF ] receptor ) was administered . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Using real time PCR , we found that VEGF , at 20 ng / ml , significantly increased the expression of VEGFR 1 and VEGFR 2 by approximately 4 fold and 31 fold , respectively , compared to untreated control ( p < 0 . 05 ) . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
However , only the combination of VEGF with LH , E 2 , and P 4 upregulated mRNA for Ace 1 and Ang 2 release , but not VEGF alone . ( 2 ) Treatments of LH , with E 2 and / or P 4 increased the mRNA expression for VEGF , Flk 1 and Flt 1 , and ( 3 ) VEGF itself downregulated the expression of mRNA for VEGF , and LH , E 2 , and P 4 enhanced this downregulatory effect . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Recent studies have suggested that excess secretion of a naturally occurring anti angiogenic molecule of placental origin referred to as soluble fms like tyrosine kinase 1 ( sFlt 1 , also referred to as sVEGFR 1 ) may contribute to the pathogenesis of preeclampsia . sFlt 1 acts by antagonizing two pro angiogenic molecules vascular endothelial growth factor ( VEGF ) and placental growth factor ( PlGF ) . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Expressions of nuclear HIF 1alpha , vascular endothelial growth factor ( VEGF ) and fms like tyrosine kinase 1 ( flt 1 ) were measured by Western blot and liver blood flow by a laser Doppler . ^^^ VEGF and flt 1 peaked at 120 and 12 h , respectively . ^^^ HIF 1alpha expression was preceded by VEGF and flt 1 expression and thus may be related to sinusoidal endothelial reconstruction . . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
BACKGROUND : Vascular endothelial growth factor ( VEGF ) and its tyrosine kinase family of receptors ( VEGFR ) ( Flt 1 , Flk 1 , Flt 4 ) have been implicated in vascular angiogenesis and remodelling in cerebral arteriovenous malformations ( CAVM ) . ^^^ METHODS : Surgical specimens from 26 patients undergoing CAVM resection were studied for HIF 1alpha , VEGF , Flt 1 , Flk 1 and Flt 4 . ^^^ RESULTS : VEGF , Flt 1 and Flt 4 were expressed in all specimens . ^^^ HIF 1alpha expression was significantly associated with VEGF , Flt 1 and Flk 1 expression ( p < 0 . 05 ) CONCLUSIONS : HIF 1alpha is expressed in human CAVM . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Skeletal muscle biopsies were obtained from the vastus lateralis before and at 4 h after a submaximal exercise bout for the measurement of morphometry , capillarization , VEGF , KDR , and Flt 1 in seven aged ( mean age 65 yr ) and eight young ( mean age 21 yr ) sedentary men . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
VEGF receptor 1 ( VEGFR 1 / Flt 1 ) is a high affinity tyrosine kinase ( TK ) receptor for VEGF and regulates angiogenesis as well as monocyte / macrophage functions . ^^^ To examine which VEGFR is important and to clarify how colony stimulating factor 1 ( CSF 1 ) and VEGF signals interact in osteoclastogenesis , we introduced a VEGFR 1 signaling deficiency ( Flt 1 ( TK ) / ) into op / op mice . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
The abundance of mRNAs for endothelial NO synthase ( eNOS ) , vascular endothelial growth factor ( VEGF ) , the VEGF receptor Flt 1 , and basic fibroblast growth factor ( bFGF ) in the myocardium was markedly reduced in vehicle treated DS rats compared with controls . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Feasibility of using siRNA for treatment of choroidal neovascularization has been demonstrated using siRNA directed against vascular endothelial growth factor ( VEGF ) or VEGF receptor 1 ( VEGFR 1 ) , and both of these approaches are being tested in clinical trials . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Because prostate cancer metastasis involves hematogenous and lymphatic routes , we also evaluated expression of the vascular endothelial growth factor ( VEGF ) and receptors ( VEGFR 1 , VEGFR 2 , and VEGFR 3 ) in the metastatic deposit by immunohistochemistry . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Immunohistochemical staining for vascular endothelial growth factor ( VEGF ) , its receptors Flk 1 and Flt 1 , transforming growth factor alpha ( TGFalpha ) , basic fibroblast growth factor ( bFGF ) , hypoxia inducible factor 1alpha ( Hif 1alpha ) , MIB 1 and proliferating cell nuclear antigen ( PCNA ) was performed using standard immunohistochemical techniques . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
VEGF binds two major VEGF receptors : VEGFR 1 ( flt 1 ) and VEGFR 2 ( flk 1 / KDR ) . ^^^ RESULTS : The expression of VEGF , VEGFR 1 and VEGFR 2 were significantly up regulated during allograft rejection as compared to isografts . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
We used immunohistochemistry to demonstrate expression of Neuropilin 1 in 63 malignant and 35 benign ovarian tumours and compared it to VEGF , Flt , Flk , SST expression and tumour microvessel density ( MVD ) . ^^^ VEGF , Flt , Flk and SST were expressed more highly in the epithelium of malignant and the vessels of benign lesions . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
The amounts of vascular endothelial growth factor ( VEGF ) , placenta growth factor ( PlGF ) and their antagonist , soluble fms like tyrosine kinase 1 ( sFlt 1 ) released in CT culture media were measured using ELISA . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Effects of oestradiol and tamoxifen on VEGF , soluble VEGFR 1 , and VEGFR 2 in breast cancer and endothelial cells . ^^^ Vascular endothelial growth factor ( VEGF ) , acting via the receptors VEGFR 1 and VEGFR 2 , is a key mediator of tumour angiogenesis . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Vascular endothelial growth factor ( VEGF ) and its receptors ( FLT 1 and KDR ) are overexpressed by human bladder cancer cells and tumor endothelial cells , respectively . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
We reveal that vascular endothelial growth factor receptor 1 ( FLT 1 ) modulates acute leukemia distribution within the BM , along VEGF and PlGF gradients , regulating leukemia survival and exit into the peripheral circulation . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
By immunohistochemistry and reverse transcriptase polymerase chain reaction , expression of VEGF receptor 1 ( VEGFR 1 ) , but not VEGFR 2 , was detected in murine keratinocytes during wound repair and in normal human epidermal keratinocytes ( NHEKs ) . ^^^ In vitro , VEGF stimulated the proliferation of NHEKs , an effect that could be blocked by treatment with neutralizing VEGFR 1 antibodies . ^^^ Treatment with anti VEGFR 1 antibodies also reduced the number of proliferating keratinocytes at the leading edge of the wound , suggesting that VEGF sends a proliferative signal to these cells . ^^^ Together , these data describe a novel role for VEGFR 1 in keratinocytes and suggest that VEGF may play several roles in cutaneous wound repair . . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Local VEGF , VEGFR 1 , VEGFR 2 , syndecan , and FGFR 1 levels were 16 75 % upregulated after Ad PR 39 injections as assessed by real time PCR , suggesting upregulation of VEGF and FGF pathways . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
METHODS : We examined VEGF , VEGFR 1 and VEGFR 2 expression on 34 papillary thyroid carcinomas ( PTCs ) , 18 follicular thyroid carcinomas ( FTCs ) , eight poorly differentiated thyroid carcinomas ( PDTCs ) and on a thyroid tumour derived cell line ( NPA ' 87 ) by immunohistochemistry , reverse transcriptase PCR , immunofluorescence and Western blotting . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Localization of vascular endothelial growth factor ( VEGF ) and its receptors VEGFR 1 and VEGFR 2 in bovine placentomes from implantation until term . ^^^ Interactions of vascular endothelial growth factor ( VEGF ) with its receptors VEGFR 1 and VEGFR 2 promoting angiogenesis have been described in placentation of human , mink and pig . ^^^ The presence of VEGF , VEGFR 1 and VEGFR 2 at the feto maternal interface and in vasculature indicates that in the bovine VEGF may have ( 1 ) classic functions in angiogenesis and vascular permeability , ( 2 ) growth factor properties , facilitating feto maternal exchange via paracrine action , ( 3 ) chemotactic activity on capillary endothelium , and ( 4 ) an autocrine influence on TGC migratory activity . . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
The soluble receptor of VEGF ( sVEGFR 1 ) , also known as soluble fms like tyrosine kinase 1 , is produced in the placenta and reduces VEGF activity . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Soluble vascular endothelial growth factor receptor 1 ( sFLT 1 ) mediates downregulation of FLT 1 and prevents activated neutrophils from women with preeclampsia from additional migration by VEGF . ^^^ VEGF , by binding to FLT 1 , induces leukocytic chemotaxis . ^^^ In contrast , serum levels of sFLT 1 in patients with preeclampsia are increased and , therefore , inhibit upregulation of FLT 1 in neutrophils by neutralizing VEGF . ^^^ VEGF dependent FLT 1 expression is regulated by changing FLT 1 promoter activity . ^^^ In vitro experiments demonstrated that migration of neutrophils is regulated by VEGF via FLT 1 and excess of sFLT 1 . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Vascular endothelial growth factor ( VEGF ) , binding to an appropriate receptor like FLT , is the main mitogen for endothelial cells and a strong inducer of angiogenesis . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
The mRNA expressions of VEGF and its receptors Flt 1 and Flk 1 were evaluated by semi quantitative reverse transcription PCR . ^^^ CONCLUSION : Exogenous insulin may promote SEC proliferation with an enhanced expression of VEGF and its receptor Flt 1 in regenerating rat liver after PHx . . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Taken together , our data suggest that VEGFR 1 receptor is a critical determinant of VEGFR 2 abundance , while VEGFR 2 is the key receptor directly responsible for endothelial cell signaling stimulated by VEGF . . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Levels of VEGF , VEGFR 1 and VEGFR 2 were determined by enzyme immunoassay in tumor and adjacent normal tissue samples from 39 breast cancer patients . ^^^ Direct correlations were established between VEGF and VEGFR 1 , on the one hand , and VEGF and VEGFR 2 , on the other . ^^^ VEGF and VEGFR 1 expression was consistently higher in progesterone receptor negative tumors . . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Real time RT PCR analysis showed that ( 1 ) the level of mRNA for VEGF was lower in old animals than young animals ; ( 2 ) the mRNA levels of Flt 1 and neuropilin 1 are lower in old animals than young animals , while that of KDR / Flk 1 remained unchanged with advancing age ; and ( 3 ) the levels of mRNA for angiopoietin 1 and 2 remained unchanged , while the mRNA for Tie 2 was lower in old animals than young animals . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
This effect was dependent on the binding of VEGF ( 165 ) to VEGFR 2 , as blocking antibodies to VEGFR 2 , but not VEGFR 1 , inhibited VEGF ( 165 ) induced migration . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Most biological effects of VEGF are mediated via two receptor tyrosine kinases , VEGFR 1 and VEGFR 2 , but specific VEGF isoforms also bind neuropilins ( NP ) 1 and 2 , non tyrosine kinase receptors originally identified as receptors for semaphorins , polypeptides with essential roles in neuronal patterning . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
This may in part be due to a reduced postoperative inflammation mediated angiogenic stimulus directed by the proangiogenic factor VEGF and its neutralizing receptor VEGFR 1 . ^^^ Thus , we evaluated perioperative plasma concentrations of soluble VEGF ( sVEGF ) and soluble VEGFR 1 ( sVEGFR 1 ) in patients undergoing elective colectomy . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
OBJECTIVE : Serum levels of soluble fms like tyrosine kinase 1 ( sFlt 1 ) , vascular endothelial growth factor ( VEGF ) , and placental growth factor ( PlGF ) are altered in women with clinical preeclampsia . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Vascular endothelial growth factor ( vegf ) and its receptors flk , flt 1 , and neuropilins 1 and 2 are expressed in both vascularized and avascular areas . ^^^ Several genes ( vegf , flk , flt 1 , timp 2 , possibly mmp 9 ) appear to be expressed by retinal glia . . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Interestingly , GDMEC expressed higher levels of VEGF receptors , flt 1 and flk 1 , as compared to an established human EC cell line ECV 304 and primary human umbilical vascular EC ( HUVEC ) . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Vascular endothelial growth factor ( VEGF ) and its receptors VEGFR 1 , soluble ( s ) VEGFR 1 and VEGFR 2 represent a regulatory system , essential for both physiological and pathological angiogenesis . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Analysis of the subcellular distribution of VEGFR 1 revealed the appearance of an 80 kDa C terminal domain in the cytosol of cells treated with VEGF and PEDF that correlated with a decrease of the full length receptor in the nuclear and cytoskeletal fractions . ^^^ This regulated intramembrane proteolysis is dependent on gamma secretase because inhibition of gamma secretase abolished the inhibitory effect of PEDF on VEGF induced angiogenesis as well as VEGFR 1 cleavage . ^^^ PEDF was also able to inhibit VEGF induced phosphorylation of VEGFR 1 . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Serum levels of vascular endothelial growth factor ( VEGF ) , soluble Flt 1 and Flk 1 receptors , placental growth factor ( PlGF ) , angiopoietin 2 ( Ang 2 ) and soluble Tie 2 receptor were determined by ELISA . ^^^ Interestingly , in CHC patients serum levels of VEGF and Tie 2 correlated with grade of inflammation , PlGF correlated with stage of fibrosis , and Flt 1 and Flk 1 correlated with serum transaminase levels ( p < 0 . 05 in all cases ) . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
OBJECTIVE : In order to clarify the role of vascular growth factor in the modulation of the vascular network of the cochlea , we studied the expression of VEGF and its receptors fms like tyrosine kinase ( Flt 1 ) and foetal liver kinase ( Flk 1 ) in the inner ear of 3 month old rodents of different species : C57BL / 6J mice , Wistar albino rats and Hartley albino guinea pigs . ^^^ In particular , VEGF and Flt 1 are present at the level of the modiolus , spiral ganglion , spiral ligament , basilar membrane , supporting cells , outer and inner hair cells and stria vascularis . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
This study tests the hypothesis that abnormalities in plasma indices of angiogenesis , such as Vascular Endothelial Growth Factor ( VEGF ) and angiopoietins ( Ang 1 , Ang 2 ) , as well as their soluble receptors Flt 1 ( sFlt 1 ) and Tie 2 ( sTie 2 ) respectively , are present in women with in pregnancy induced hypertension ( PIH ) . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
The present study sought to identify the sequence of events and the fate of endothelial cells , pericytes , and vascular basement membrane during capillary regression in mouse tracheas after VEGF signaling was blocked with a VEGF receptor tyrosine kinase inhibitor AG 013736 or soluble receptor construct ( VEGF Trap or soluble adenoviral VEGFR 1 ) . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Immunoreactivity for vascular endothelial growth factor ( VEGF ) and its receptor , flt 1 , was observed in the endothelial cells of both intratumoral vessels and cyst wall MVPs . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
We have previously shown expression of vascular endothelial growth factor ( VEGF ) , a major regulator of endothelial cell proliferation , angiogenesis and vascular permeability , and VEGF receptors ( VEGFR 1 and 2 ) after TBI in rat . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
A SNP in the flt 1 promoter integrates the VEGF system into the p 53 transcriptional network . ^^^ A target of VEGF , the VEGF receptor 1 / flt 1 , can regulate growth and migration of endothelial cells and modulate angiogenesis . ^^^ VEGF appears to be up regulated in various cancers in which flt 1 may have a role in tumor progression and metastasis . ^^^ Our results establish that p 53 can differentially stimulate transcription at a polymorphic variant of the flt 1 promoter and directly places the VEGF system in the p 53 stress response network via flt 1 in a significant fraction of the human population . ^^^ We suggest that the p 53 VEGF flt 1 interaction is relevant to risks in angiogenesis associated diseases , including cancer . . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
A growing body of observations , including the side effects of anti VEGF therapies as well as the role of soluble VEGFR 1 in preeclampsia , points to an important role for VEGF in maintenance of stable blood vessels . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
PURPOSE : To evaluate whether transfection of human retinal endothelial cells ( HRECs ) with plasmids expressing ribozymes designed to specifically cleave the mRNA and reduce expression of either vascular endothelial growth factor ( VEGF ) receptor 1 ( VEGFR 1 ) , or VEGF receptor 2 ( VEGFR 2 ) , or insulin like growth factor 1 receptor ( IGF IR ) modulates occludin expression in high glucose treated cells . ^^^ VEGF and IGF 1 levels were measured in conditioned medium of HREC exposed to high glucose conditions , and the effect of varying glucose concentration on VEGFR 1 and VEGFR 2 phosphorylation was examined . ^^^ RESULTS : Exposure of HRECs to high glucose resulted in increased VEGF and IGF 1 expression as well as VEGFR 2 but not VEGFR 1 phosphorylation . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
PET ( pre eclamptic toxaemia ) has recently been linked with alterations in production of a VEGFR 1 [ VEGF ( vascular endothelial growth factor ) receptor 1 ] splice variant that acts as a circulating inhibitor . ^^^ To determine whether alteration in splicing of VEGF VEGFR family members extended beyond VEGFR 1 , we investigated the effect of pre eclampsia on placental VEGFxxxb mRNA and protein expression . ^^^ Combined with previous studies showing increased soluble VEGFR 1 isoforms in human pre eclampsia , these data suggest that there may be a common mechanism in pre eclampsia that involves dysregulation of mRNA splicing of members of the VEGF VEGFR axis . . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
AML M 3 having t ( 15 ; 17 ) abnormality showed highest expression of VEGF and VEGF receptor type 1 ( VEGFR 1 ) , suggesting the autocrine pathway of VEGF VEGFR 1 . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
The purpose of this animal study was to evaluate the effects of cyclical tendon loading on the densities of cells producing growth factors such as vascular endothelial growth factor ( VEGF ) , its receptor , vascular endothelial growth factor receptor 1 ( VEGFR 1 ) , and connective tissue growth factor ( CTGF ) in the Flexor Digitorum Profundus ( FDP ) tendon at the epicondyle . ^^^ VEGF , VEGFR 1 , and CTGF cell densities in tendon are increased with cyclical loading : An in vivo tendinopathy model . ^^^ The tendons at the medial epicondyle insertion sites were harvested , and sections were immunostained with antibodies directed against VEGF , VEGFR 1 , or CTGF . ^^^ VEGF ( p = 0 . 0001 ) , VEGFR 1 ( p = 0 . 046 ) , and CTGF ( p = 0 . 0001 ) cell densities were increased in the tendon of the loaded limb compared to the nonloaded limb . ^^^ In addition , regional differences in VEGF , VEGFR 1 , and CTGF cell densities were found . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Expression and clinical significance of VEGF and its receptors Flt 1 and KDR in nasopharyngeal carcinoma ] . ^^^ BACKGROUND & OBJECTIVE : Vascular endothelial growth factor ( VEGF ) and its receptors fms like tyrosine kinase 1 ( Flt 1 ) and kinase insert domain containing receptor ( KDR ) play critical roles in angiogenesis and tumor progression . ^^^ This study was to explore the correlations of VEGF , Flt 1 and KDR expression to clinical features and prognosis of nasopharyngeal carcinoma ( NPC ) patients . ^^^ METHODS : Immunohistochemistry was adopted to detect the expression of VEGF , Flt 1 , and KDR in 127 specimens of NPC . ^^^ RESULTS : The positive rates of VEGF , Flt 1 , and KDR were 66 . 9 % , 90 . 6 % , and 88 . 2 % in the NPC specimens . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Furthermore , by using real time PCR analysis , thymosin beta 10 significantly decreased mRNA levels of vascular endothelial growth factor ( VEGF ) , VEGF receptor 1 ( VEGFR 1 ) and integrin alphaV after 24 h treatment in HCAECs . ^^^ These results indicate that thymosin beta 10 , but not thymosin beta 4 , have direct inhibitive effects on endothelial migration and tube formation that might be mediated via downregulation of VEGF , VEGFR 1 and integrin alphaV in HCAECs . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
VEGF activates VEGF receptor 1 ( VEGFR 1 ) and VEGFR 2 , whereas VEGF C activates VEGFR 2 and VEGFR 3 . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
OBJECTIVES : We have examined VEGF and its receptors , VEGFR 1 and VEGFR 2 , and angiopoietin 1 ( Ang 1 ) in the airways of subjects with asthma and contrasted these results with findings in normal control subjects . ^^^ MEASUREMENTS : We performed immunohistochemistry and image analysis to obtain quantitative measures of VEGF , VEGFR 1 , VEGFR 2 , and Ang 1 staining in airway biopsies , and ELISA to assess VEGF concentration in the bronchoalveolar lavage fluid . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
The objective of this study was to use a series of algorithms called AQUA that quantitatively assesses protein expression on tissue microarrays ( TMAs ) to compare in situ expression of VEGF and its primary receptors , VEGF receptor 1 ( FLT 1 ) and VEGF receptor 1 ( FLK 1 ) , on a pancreatic cancer TMA . ^^^ The primary antibodies used were VEGF , FLT 1 , FLK 1 , and cytokeratin . ^^^ A significant association was found between low expression of FLT 1 and both poor prognosis and advanced stage , suggesting that tumor expression of this VEGF receptor is a marker of less aggressive disease . . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Rats underwent RT PCR determination of one of the VEGF receptors flt 1mRNA , and immunohistochemistry for VEGF receptors Flt 1 and Flk 1 . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
VEGF receptor 1 , 2 , and 3 ( VEGFR 1 , VEGFR 2 , and VEGFR 3 , respectively ) expression increased throughout the time course , with maximal expression at 48 , 8 , and 72 h , respectively . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Five micrometers sections were submitted to standard immunohistochemistry protocol using three primary antibodies ( SC 315 , SC 316 and VG76e ) for detection of kinase domain region ( KDR ) , fms like tyrosine kinase 1 ( Flt 1 ) and VEGF , respectively . ^^^ We concluded that VEGF might have a modulatory effect in the CL of the dog acting as paracrine and autocrine factor through its receptors , Flt 1 and KDR . . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
This response is dependent upon the expression of vascular endothelial growth factor ( VEGF ) receptor 1 ( VEGFR 1 ) , which mediates cell migration in response to VEGF or placental growth factor ( PLGF ) . ^^^ In this study , we found that exposure of cultured mouse bone marrow derived mesenchymal stromal cells ( MSC ) to hypoxia or an adenovirus encoding a constitutively active form of hypoxia inducible factor 1 ( HIF 1 ) induced VEGFR 1 mRNA and protein expression and promoted ex vivo migration in response to VEGF or PLGF . ^^^ MSC in which HIF 1 activity was inhibited by a dominant negative or RNA interference approach expressed markedly reduced levels of VEGFR 1 and failed to migrate or activate AKT in response to VEGF or PLGF . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
VEGF , VEGFR 1 and VEGFR 2 immunoreactivity in the porcine arteries of vascular subovarian plexus ( VSP ) during the estrous cycle . ^^^ The aim of the present study was to immunolocalize VEGF and its two receptors : VEGFR 1 and VEGFR 2 in the broad ligament of the uterus in the area of vascular subovarian plexus during different phases of the estrous cycle in pig and to determine the correlation between immunoreactivity of the investigated factors and phases of the estrous cycle . ^^^ Specific polyclonal antibodies : anti VEGF , anti VEGFR 1 and anti VEGFR 2 were used . ^^^ All agents displayed phase related differences in immunoreactivity suggesting the modulatory effect of VEGF , VEGFR 1 and VEGFR 2 on the arteries of the VSP in the porcine broad ligament of the uterus . . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
In the present study , we investigated the antitumor effect of an adenovirus ( AdVEGF ExR ) expressing the extracellular domain of the human VEGF receptor ( flt 1 ) using two different urological tumor / mouse systems . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
We found that the pR F dicistronic reporter containing the 5 ' UTRs from HIF 1alpha , VEGF , c myc , XIAP , VEGFR 1 , or Egr 1 UTRs all produce the downstream luciferase predominantly as a result of cryptic promoter activity that is activated by the SV 40 enhancer elements in the plasmid . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Our study analyzed the expression of Vascular Endothelial Growth Factor ( VEGF ) and its functional receptors , Flt 1 and Flk 1 , in the cochlear structures of noise exposed and unexposed guinea pigs . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
OBJECTIVE : To evaluate the effect of hypoxia on the production of vascular endothelial growth factor ( VEGF ) and soluble fms like tyrosine kinase 1 ( sFlt 1 ) in the human fallopian tube . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
We induced apoptosis of microvascular EC using shear stress and the combined VEGF receptor ( VEGFR 1 and 2 ) inhibitor SU 5416 . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
The mRNA and protein expressions of VEGF and of fms like tyrosine kinase 1 ( Flt 1 ) and fetal liver kinase 1 ( Flk 1 ) , which are main VEGF receptors , in the heart were significantly lower in the sedentary aged rats compared with the sedentary young rats , whereas those in the exercise trained rats were significantly higher than those in the sedentary aged rats . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Alterations in the placental bed expression of VEGFR 1 , VEGFR 2 , and Tie 1 , but not of VEGF and Tie 2 , may be associated with PE , IUGR , or DM . . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
To investigate the possible role of angiogenic mechanisms , we have studied the immunohistochemical expression of vascular endothelial growth factor ( VEGF ) , angiopoietin 1 ( Ang 1 ) , angiopoietin 2 ( Ang 2 ) and their receptors ( VEGFR 1 , VEGFR 2 , Tie 2 ) in ADPKD , Caroli ' s disease , normal and fetal livers . ^^^ Cholangiocytes were strongly positive for VEGF , VEGFR 1 , VEGFR 2 and Ang 2 in ADPKD and Caroli , and also for Ang 1 and Tie 2 in ADPKD , similar to fetal ductal plate cells . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Moreover , both these processes share similar modulating factors , like vascular endothelial growth factor ( VEGF ) and its receptors VEGFR 1 ( Flt 1 ) and VEGFR 2 ( Flk 1 ) . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
The soluble form of the VEGF receptor 1 ( soluble fms like tyrosine kinase 1 ( sFlt 1 ) ) is known to regulate VEGF activity by binding VEGF in the circulation . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Single loop mutants did not bind to either receptor , whereas a VEGF E mutant in which both L 1 and L 3 were replaced gained affinity for VEGFR 1 , illustrating the possibility to engineer receptor specific chimeric VEGF molecules . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Further , VEGF stimulation resulted in enhanced cellular migration , which was effectively blocked by pharmacologic inhibition of VEGFR 1 or Src kinase . ^^^ Furthermore , VEGF treatment enhanced VEGFR 1 / SFK complex formation and increased tyrosine phosphorylation of focal adhesion kinase , p 130 cas and paxillin . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
VEGFA ( VEGF ) , VEGFB , VEGFC , VEGFD ( FIGF ) and PGF ( PlGF ) are VEGF family ligands for receptor tyrosine kinases , including VEGFR 1 ( FLT 1 ) , VEGFR 2 ( KDR ) and VEGFR 3 ( FLT 4 ) . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Study of microvessel density and the expression of the angiogenic factors VEGF , bFGF and the receptors Flt 1 and FLK 1 in benign , premalignant and malignant prostate tissues . ^^^ VEGF exerts its action by binding to the specific cell surface receptors , fms like tyrosine kinase 1 ( Flt 1 ) and fetal liver kinase 1 ( FLK / KDR ) . ^^^ Very little information on the expression of VEGF , bFGF , and the receptors Flt 1 and FLK / KDR is available . ^^^ MATERIALS AND METHODS : 58 selected surgical specimens exhibiting areas of normal prostate , HGPIN , and CaP were evaluated for microvessel density , and for VEGF , bFGF , Flt 1 and FLK / KDR protein expression by immunohistochemistry . ^^^ RESULTS : There was a statistically significant increase in the microvessel density and in the expression of the angiogenic factors VEGF , bFGF and the receptors FLK / KDR and Flt 1 , in the premalignant and malignant tissues in comparison with normal prostatic glands . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
PATIENTS AND METHODS : We examined the prognostic value of the expression of vascular endothelial growth factor ( VEGF ) and of the VEGF receptors ( VEGFRs ) fms like tyrosine kinase receptor 1 ( flt 1 ) and kinase insert domain containing receptor ( KDR ) in non small cell lung cancer ( NSCLC ) . ^^^ In contrast , flt 1 expression was not correlated with VEGF expression or outcome . ^^^ Multivariate analysis identified expression of both flt 1 and KDR and VEGF and KDR as possible independent prognostic factors . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
We found that VEGF receptor FMS like tyrosine kinase 1 ( Flt 1 ) and fetal liver kinase 1 ( Flk 1 ) expression increased during ESC differentiation . ^^^ Antibodies against Flk 1 totally blocked and against Flt 1 partially blocked VEGF induced NKx2 . 5 positive stained cells . ^^^ Our results suggest that VEGF promotes cardiomyocyte differentiation predominantly by ERK mediated Flk 1 activation and , to a lesser extent , by Flt 1 activation . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Adenovirus mediated overexpression of soluble Flt 1 ( sFlt 1 ) in a mouse model of endotoxemia attenuated the rise in VEGF and PlGF levels and blocked the effect of endotoxemia on cardiac function , vascular permeability , and mortality . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Vascular endothelial growth factor ( VEGF ) and VEGF receptor 1 ( VEGFR 1 / Flt 1 ) were shown to be involved in pathological angiogenesis , particularly rheumatoid arthritis ( RA ) . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
RTQ PCR demonstrated that VEGF , its receptors VEGFR 1 and VEGFR 2 , and angiopoietin 2 and its receptor ( Tie 2 ) were also overexpressed ( P < 0 . 05 ) in PTC . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Using samples of CCE and COE , we immunohistochemically determined protein expression of the angiogenesis related factors : Thrombospondin 1 ( TSP 1 ) , pigment epithelium derived factor ( PEDF ) , endostatin , angiostatin , vascular endothelial growth factor ( VEGF ) , Fms like tyrosine kinase 1 ( Flt 1 ) , kinase insert domain receptor ( KDR ) , and basic fibroblast growth factor ( bFGF ) . ^^^ Immunohistochemically there was no obvious difference between CCE and COE with respect to angiostatin , VEGF , Flt 1 , KDR , and bFGF . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Two high affinity receptors for vascular endothelial growth factor ( VEGF ) A , VEGFR 1 and VEGFR 2 , cooperate for physiological vasculogenesis and angiogenesis in embryogenesis . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
There was a significant down regulation of VEGF and VEGFR 2 ( KDR ) but not VEGFR 1 ( Flt 1 ) gene expression by chrysin in LPS treated HUVEC cultures . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
In association with these cellular changes , mRNA for several angiogenic factors including vascular endothelial growth factor ( VEGF ) and receptors ( Flt and KDR ) , basic fibroblast growth factor ( FGF 2 ) and receptor , angiopoietin ( ANGPT ) 1 and receptor Tie 2 , endothelial nitric oxide synthase ( NOS 3 ) , and angiotensin 2 receptor 1 ( AT 1 ) were altered . ^^^ In addition , changes in mRNA expression for VEGF , Flt and KDR were positively correlated with changes in ANGPT 2 , Tie 2 , and NOS 3 , indicating a functional relationship . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
The body weight was decreased in the diabetic rats compared with the control rats , but the left ventricular ( LV ) body weight ratio was increased in the diabetic group and was unaffected by treatment with ET antagonist . mRNA expression of VEGF and its receptors ( Flt 1 and Flk 1 ) in the LV tissues was assessed using real time polymerase chain reaction . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Expression of Flt 1 , the vascular permeability receptor of VEGF , was also increased in liver tissue after LPS administration . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Previous studies suggest that the effect of VEGF on endothelial cell migration is primarily mediated via VEGFR 2 ; however , VEGF induced EPC migration in vitro was mediated by both receptors , suggesting that VEGF VEGFR 1 interactions in EPCs are distinct from differentiated endothelial cells . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
CONCLUSIONS : These results indicated that neither the hyperpermeability in response to simultaneous stimulation of VEGFR 1 and VEGFR 2 nor VEGFR 1 mediated severe inflammation was associated with VEGF E ( NZ 7 ) / PlGF induced angiogenesis . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Retinal mRNA expression of VEGF splice variants ( VEGF 188 , VEGF 164 , VEGF 120 ) , VEGF receptors ( VEGFR 1 , VEGFR 2 , Npn 1 , Npn 2 ) , and pigment epithelium derived factor ( PEDF ) were also examined . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
In the present study , VEGF specific membrane receptor ( VEGFR 1 / Flt 1 and VEGFR 2 / Flk 1 ) expression was studied by confocal immunofluorescence in the corpus cavernosum of control rats , rats aged 12 and 18 months , and orchidectomized Wistar rats ( 90 days of bilateral orchidectomy ) . ^^^ Aging and orchidectomy modulate expression of VEGF receptors ( Flt 1 and Flk 1 ) on corpus cavernosum of the rat . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
The increase of flk 1 and flt 1 ( VEGF receptors ) and tie 2 ( Ang 1 receptor ) induced by ang 2 was significantly suppressed by PD 123319 . ^^^ The increase of VEGF / flk 1 / flt 1 may be associated with vascular permeability , monocyte / macrophage infiltration , and rarefaction of peritubular capillaries , and the increase of the Ang1 / Ang2 ratio may be a compensatory mechanism counteracting the permeability inducing effect of VEGF after ang 2 infusion . . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Association between UHMWPE particle induced inflammatory osteoclastogenesis and expression of RANKL , VEGF , and Flt 1 in vivo . ^^^ The purpose of this study is to investigate the association of ultra high molecular weight polyethylene ( UHMWPE ) particle induced inflammatory osteoclastogenesis and expression of RANK / RANKL and VEGF / VEGF receptors ( Flt 1 and Flk 1 ) using a mouse osteolysis model . ^^^ UHMWPE stimulation significantly increased VEGF gene expression , and exerted a lower enhancement effect on the gene expression of Flt 1 and Flk 1 . ^^^ Immunofluorescent staining indicated that pouch tissue macrophages were the main source of both VEGF and Flt 1 production . ^^^ A positive association was observed between tissue inflammation and the levels of VEGF and Flt 1 gene transcripts . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
OBJECTIVES : This study examined whether atorvastatin increases plasma levels of soluble Fms like tyrosine kinase 1 ( sFlt 1 ) and reciprocally decreases vascular endothelial growth factor ( VEGF ) and placental growth factor ( PlGF ) levels in patients with acute myocardial infarction ( AMI ) . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Immunolocalizations of VEGF , its receptors flt 1 , KDR and TGF beta ' s in epithelial ovarian tumors . ^^^ In this study , we aimed to evaluate the immunolocalizations of vascular endothelial growth factor ( VEGF ) , its receptors flt 1 , KDR / flk 1 , and transforming growth factor beta ' s ( TGF beta ) in epithelial ovarian tumors , utilizing indirect immunohistochemistry to understand the role of the angiogenic events in ovarian neoplasia . ^^^ All formalin fixed , paraffin embedded tissue sections were stained with hematoxylin eosin or primary antibodies against VEGF , flt 1 , KDR / flk 1 , TGF beta 1 , TGF beta 2 and TGF beta 3 using the avidin biotin peroxidase method . ^^^ VEGF receptors , flt 1 and KDR / flk 1 immunoreactivities were detected not only in vascular endothelial cells , but also in tumor cells at malignant sites . ^^^ Conclusion : Our results suggest that overexpression of VEGF , its receptors flt 1 , KDR / flk 1 and TGF beta interaction may play an important role in the ovarian cancer biology , with potential effects on tumor growth and angiogenesis . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
The soluble isoform of VEGF is a dimeric glycoprotein of 36 46 kDa , induced by hypoxia and oncogenic mutation and it binds to two specific tyrosine kinase receptors : VEGF 1 ( flt 1 ) and VEGF 2 ( KDR / flk1 ) . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Placenta growth factor binds only to VEGF receptor 1 ( FLT 1 ) . ^^^ It has been postulated that PlGF is involved in intra and intermolecular cross talk between VEGF receptor 1 ( FLT ) and receptor 2 ( FLK 1 / KDR ) . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Blocking associations of the VEGF with its corresponding receptors ( Flt 1 , KDR / flk 1 ) have become critical for anti tumor angiogenesis therapy . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Vascular endothelial growth factor ( VEGF ) and its receptor tyrosine kinases , VEGFR 1 and VEGFR 2 , are important therapeutic targets for various cancers including AML . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Cell viability , VEGF and VEGF receptor ( VEGFR 1 and VEGFR 2 ) expression , and transcription factor activation were studied on transient exposure of monolayer EC to H2O2 . ^^^ Exposure to H2O2 increased VEGF and VEGFR 2 mRNA and protein in EC , without affecting VEGFR 1 expression . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
The analysis of mRNA expression of vascular endothelial growth factors ( VEGF ) and their receptors ( VEGFR 1 and VEGFR 2 ) showed a significant decrease in each VEGF isoform and in VEGFR 2 mRNA in representative alveolar wall tissues microdissected from the normal , NSIP , and UIP lungs . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
It was found that , in addition to the secretion of angiogenic factors VEGF and b FGF by B CLL and MM cells , MM cells ( but not B CLL cells ) induced a dramatic increase in expression of VEGFR 1 and VEGFR 3 on human bone marrow endothelial cells ( HBMEC ) . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Furthermore , five genes related to vascular endothelial cell physiology ( CTGF , WWP 2 , CD 274 , VEGF . and its receptor FLT 1 ) showed significantly reduced expression in the postperfusion biopsies of patients with need of therapeutic interventions due to graft related complications in the first month ( P < 0 . 05 ) . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
AIM : To investigate H pylori expression in gastric cancer patients in relation to primary tumor angiogenic markers , such as microvessel density ( MVD ) , thymidine phosphorylase ( TP ) , vascular endothelial growth factor receptor 1 ( VEGF R 1 ) , p 53 and circulating VEGF levels . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
VEGF receptor 2 ( VEGFR 2 ) is critical for angiogenesis , although little is known about the precise role of endothelial VEGFR 1 and its downstream effectors in this process . ^^^ Consistent with these findings , the VEGFR 1 specific ligand placenta growth factor 1 activated phosphatidylinositol 3 kinase and VEGF E , which is selective for VEGFR 2 activated phospholipase Cgamma 1 . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Frequently vascular endothelial cell growth factors ( VEGFs ) are mainly responsible for the pathological neovascularization as in the case in neovascularization induced by CpG oligodeoxynucleotides and herpes simplex virus infection in this report . siRNAs targeting either VEGFA , VEGFR 1 , VEGFR 2 , or a mix of the three were shown to significantly inhibit neovascularization induced by CpG when given locally or systemically . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
Up regulated genes include vasculogenic growth factors such as VEGFA , VEGFC , FIGF ( VEGFD ) , ANG 1 , ANG 2 , TGFbeta 3 , and PDGFB , as well as the related receptors FLT 1 , FLT 4 , PDGFRB , TGFbetaR 2 , and TGFbetaR 3 , other markers such as CD 34 , VCAM 1 , PECAM 1 , VE CAD , and transcription factors TAL 1 , GATA 2 , and GATA 3 . ^^^
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
NA
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
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Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
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Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
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Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
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Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
NA
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
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Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
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Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
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Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
NA
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
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Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
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Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
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Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
NA
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
NA
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
NA
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
NA
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
NA
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
NA
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
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Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
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Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
NA
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
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Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
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Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
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Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
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Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
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Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
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Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
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Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
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Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
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Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
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Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
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Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
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Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
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Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
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Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
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Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
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Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
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Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
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Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
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Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
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Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
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Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
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Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
NA
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
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Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
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Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
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Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
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Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
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Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
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Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
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Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
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Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
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Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
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Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
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Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
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Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
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Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
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Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
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Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
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Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
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Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
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Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
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Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
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Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
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Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
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Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
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Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
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Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
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Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
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Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
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Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
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Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
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Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
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Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
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Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
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Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
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Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
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Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
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Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
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Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
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Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
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Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
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Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
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Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
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Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
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Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
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Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
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Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
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Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
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Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
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Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
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Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
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Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
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Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
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Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
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Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
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Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
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Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
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Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
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Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
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Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
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Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
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Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
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Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
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Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
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Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
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Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
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Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
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Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
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Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
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Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
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Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
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Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
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Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
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Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
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Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
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Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
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Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
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Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
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Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
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Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
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Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
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Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
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Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
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Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
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Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
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Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
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Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
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Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
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Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
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Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
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Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
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Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
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Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
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Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
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Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
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Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
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Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
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Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
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Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
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Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
NA
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
NA
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
NA
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
NA
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
NA
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
NA
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
NA
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
NA
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
NA
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
NA
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
NA
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
NA
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
NA
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
NA
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
NA
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
NA
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
NA
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
NA
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
NA
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
NA
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
NA
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
NA
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
NA
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
NA
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
NA
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
NA
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
NA
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
NA
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
NA
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
NA
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
NA
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
NA
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
NA
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
NA
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
NA
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
NA
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
NA
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
NA
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
NA
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
NA
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
NA
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
NA
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
NA
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
NA
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
NA
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
NA
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
NA
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
NA
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
NA
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
NA
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
NA
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
NA
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
NA
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
NA
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
NA
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
NA
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
NA
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
NA
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
NA
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
NA
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
NA
Interacting proteins: P15692 and P17948	Pubmed	SVM Score :0.0
NA