Pubmed abstracts for Protein-Protein Interaction search result :

Interacting proteins: P15538 and P19099	Pubmed	SVM Score :0.0
Restriction fragment length polymorphism analysis using a P450c11 probe demonstrates that a MspI restriction site mutation within the CYP11B gene can not be the underlying cause for this defect , as has been suggested previously . . ^^^
Interacting proteins: P15538 and P19099	Pubmed	SVM Score :0.0
P450c11 is encoded by the CYP11B1 gene which is located on chromosome 8q22 along with a homologous gene of unknown function , CYP11B2 . ^^^
Interacting proteins: P15538 and P19099	Pubmed	SVM Score :0.0
The P450c21 gene , CYP21B , and a pseudogene , CYP21A , are located in the HLA major histocompatibility complex on chromosome 6p , while the P450c11 gene , CYP11B , is on chromosome 8q along with a second related gene of unknown function . ^^^
Interacting proteins: P15538 and P19099	Pubmed	SVM Score :0.0
A second P450c11 gene ( CYP11B2 ) encodes P450c11AS , which is the aldosterone synthase found solely in the adrenal zona glomerulosa . ^^^
Interacting proteins: P15538 and P19099	Pubmed	SVM Score :0.0
These results demonstrate that intact P450c11 was not produced at all due to the nonsense mutation in CYP11B1 of the patient without any mutation in CYP11B2 . . ^^^
Interacting proteins: P15538 and P19099	Pubmed	SVM Score :0.0
CYP11B1 encodes a specific cytochrome P 450 ( P 450c11 ) necessary for cortisol biosynthesis , with predominantly 11 beta hydroxylase and moderate 18 hydroxylase activity , whereas CYP11B2 encodes another isozyme ( P 450cmo ) necessary for aldosterone biosynthesis , with 11 beta hydroxylase , 18 hydroxylase and 18 oxidase activities ( the latter two termed corticosterone methyl oxidase 1 and 2 ; CMO 1 and 2 , respectively ) . ^^^
Interacting proteins: P15538 and P19099	Pubmed	SVM Score :0.0
In three of four unrelated P450c11AS alleles from two unrelated patients with CMOII deficiency , we found a gene conversion event in which exons 3 and 4 of the CYP11B2 gene encoding P450c11AS were changed to the sequence of the nearby CYP11B1 gene , which encodes the related enzyme P450c11 beta . ^^^
Interacting proteins: P15538 and P19099	Pubmed	SVM Score :0.0
The hamster adrenal cytochrome P450C11 has equipotent 11beta hydroxylase and 19 hydroxylase activities , but no aldosterone synthase activity . ^^^ Both P450C11 and P450aldo cDNA coding sequences were inserted in the plasmid pBluescript SK , transcribed and then translated using a rabbit reticulocyte system in the presence of [ 35S ] methionine . ^^^ The reaction products were immunoprecipitated with an anti bovine P450C11 antibody for P450C11 and with an anti hamster P450aldo for P450aldo . ^^^ A single 35S labeled protein band was detected for P450C11 and for P450aldo , respectively . ^^^ P450C11 and P450aldo cDNAs were then both inserted into the expression vector pCMV 5 containing a viral sequence specific for the attachment of ribosomes to mRNA . ^^^
Interacting proteins: P15538 and P19099	Pubmed	SVM Score :0.0
Two human isozymes are known : P450c11 beta , encoded by the CYP11B1 gene , has 11 beta hydroxylase activity ; P450c11AS , encoded by the CYP11B2 gene , has 11 beta hydroxylase , 18 hydroxylase , and aldosterone synthase activities . ^^^ The human genome contains only two CYP11B ( P450c11 ) genes . ^^^ As the number of human CYP11B genes was unknown and as the existence of novel P450c11 isozymes might have implications in the study of hypertension , we sought to determine if the human genome , like the rat genome , contained more than two CYP11B genes . ^^^
Interacting proteins: P15538 and P19099	Pubmed	SVM Score :0.0
We detected mRNAs for adrenodoxin , P450scc ( cholesterol side chain cleavage enzyme ) and P450c11 beta ( 11 beta hydroxylase ) but not for P450c17 ( 17 alpha hydroxylase / 17 , 20 lyase ) or P450c11AS ( aldosterone synthase ) in rat brains and cultures of rat glial cells . ^^^
Interacting proteins: P15538 and P19099	Pubmed	SVM Score :0.0
H295R , was utilized in this study to examine the intracellular second messenger pathways regulating expression of P450aldo and P450c11 . using specific ribonuclease protection assays . ^^^ While K+ treatment was more specific for the induction of P450aldo mRNA , treatment with angiotensin 2 increased levels of both P450aldo and P450c11 transcripts . ^^^ To define the second messenger systems which influence transcript levels for these enzymes , the effects of agonists of the protein kinase A , protein kinase C , and calcium pathways were tested on the expression of P450aldo and P450c11 . ^^^ Activation of the protein kinase A pathway by the agonists , dibutyryl cAMP or forskolin , preferentially increased the P450c11 transcript to a greater degree than P450aldo . ^^^ Interestingly , activation of the protein kinase C pathway by tetradecanoylphorbol acetate ( TPA ) did not alter transcripts for either P450aldo or P450c11 . ^^^
Interacting proteins: P15538 and P19099	Pubmed	SVM Score :0.0
Specific zonal localization and developmental regulation of CYP21A2 ( P450c21 ) and CYP11B1 / CYP11B2 ( P450c11 / aldosterone synthase ) lead to integrated concept of zonal and temporal steroid biosynthesis . ^^^ The enzymes CYP21A2 ( P 450 21 hydroxylase , or P450c21 ) , CYP11B1 ( 11beta hydroxylase or P450c11 ) and CYP11B2 ( aldosterone synthase ) are necessary for glucocorticoid and mineralocorticoid synthesis but have not been localized previously in an ontogenic manner in the primate fetal adrenal gland . ^^^
Interacting proteins: P15538 and P19099	Pubmed	SVM Score :0.0
The cDNAs showed 96 % and 94 . 6 % sequence identity to human P450c11 and aldosterone synthase , respectively . ^^^
Interacting proteins: P15538 and P19099	Pubmed	SVM Score :0.0
Further investigation of Compound A indicated that it is a mixed inhibitor of sheep P450c11 with a stronger competitive ( Kic = 106 110 microM ) than uncompetitive ( Kiu = 667 737 microM ) element , and that it inhibits the conversion of deoxycorticosterone to corticosterone by human 11beta hydroxylase and aldosterone synthase with EC 50 values of 97 microM and 190 microM , respectively , in fetal calf serum . . ^^^
Interacting proteins: P15538 and P19099	Pubmed	SVM Score :0.0
Patients with glucocorticoid remediable aldosteronism ( GRA ) from 12 kindreds possess chimaeric gene duplications arising from unequal crossing over , fusing regulatory sequences of steroid 11 beta hydroxylase to coding sequences of aldosterone synthase . ^^^
Interacting proteins: P15538 and P19099	Pubmed	SVM Score :0.0
We proposed that a cell selective regulatory protein coordinately regulates the expression of three enzymes that are required for the biosynthesis of corticosteroids : cholesterol side chain cleavage enzyme , steroid 21 hydroxylase , and the aldosterone synthase isozyme of steroid 11 beta hydroxylase . ^^^
Interacting proteins: P15538 and P19099	Pubmed	SVM Score :0.0
Molecular biology of rat steroid 11 beta hydroxylase [ P 450 ( 11 beta ) ] and aldosterone synthase [ P 450 ( 11 beta , aldo ) ] . ^^^ The molecular features of rat steroid 11 beta hydroxylase [ P 450 ( 11 beta ) ] and aldosterone synthase [ P 450 ( 11 beta , aldo ) ] are discussed . ^^^
Interacting proteins: P15538 and P19099	Pubmed	SVM Score :0.0
Genes encoding aldosterone synthase and steroid 11 beta hydroxylase ( expressed in both adrenal fasciculata and glomerulosa ) , which are 95 % identical and lie on chromosome 8q ( refs 7 , 10 ) , are therefore candidate genes for GRA . ^^^
Interacting proteins: P15538 and P19099	Pubmed	SVM Score :0.0
It was identified as CYP11B2 , which was previously postulated to be a pseudogene or a less active gene closely related to CYP11B1 , the gene encoding steroid 11 beta hydroxylase ( P 45011 beta ) [ Mornet , E . , Dupont , J . , Vitek , A . & White , P . ^^^ P 450C18 as expressed in COS 7 cells exhibits steroid 18 hydroxylase activity to catalyze the synthesis of aldosterone and 18 oxocortisol and exhibits steroid 11 beta hydroxylase activity as well . ^^^ Role of steroid 11 beta hydroxylase and steroid 18 hydroxylase in the biosynthesis of glucocorticoids and mineralocorticoids in humans . ^^^ P 450C18 as expressed in COS 7 cells exhibits steroid 18 hydroxylase activity to catalyze the synthesis of aldosterone and 18 oxocortisol and exhibits steroid 11 beta hydroxylase activity as well . ^^^
Interacting proteins: P15538 and P19099	Pubmed	SVM Score :0.0
For example , an autosomal dominant form of human hypertension , glucocorticoid suppressible hyperaldosteronism , is caused by recombination between the genes for aldosterone synthase ( CYP11B2 ) and steroid 11 beta hydroxylase ( CYP11B1 ) , creating a chimeric gene in which the CYP11B1 promoter and CYP11B2 specific coding sequences are juxtaposed . ^^^
Interacting proteins: P15538 and P19099	Pubmed	SVM Score :0.0
Accordingly , adrenal glands are enlarged because of hypertrophy of the cortex , resulting in increased expression of key cortical steroid biosynthetic enzymes , such as side chain cleavage enzyme , steroid 11 beta hydroxylase , and aldosterone synthase . ^^^
Interacting proteins: P15538 and P19099	Pubmed	SVM Score :0.0
From hitherto identified genes located on the 8th human chromosome the author mentions the following : the lipoprotein lipase gene the pathogenic allells of which cause hyperlipoproteinaemia , the ancyrine gene responsible for the development of spherocytosis , the carboanhydrase genes responsible for the development of renal tubular acidosis and the gene of steroid 11 beta hydroxylase ( CYP11B ) which is associated with inborn adrenal hyperplasia . ^^^
Interacting proteins: P15538 and P19099	Pubmed	SVM Score :0.0
Glucocorticoid suppressible hyperaldosteronism , an inherited form of human hypertension due to the dominant inheritance of a chimaeric steroid 11 beta hydroxylase / aldosterone synthase gene , has given an insight into the possible genetic factors involved in essential hypertension . ^^^ Study of the aldosterone synthase and steroid 11 beta hydroxylase genes has shown the presence of polymorphisms in both of these genes in human subjects ; further studies may demonstrate genetic mutations with pathophysiological effects in patients with essential hypertension . . ^^^
Interacting proteins: P15538 and P19099	Pubmed	SVM Score :0.0
Recent studies demonstrate that GRA is caused by a gene duplication fusing regulatory sequences of the steroid 11 beta hydroxylase gene to the coding sequences of the aldosterone synthase gene . ^^^
Interacting proteins: P15538 and P19099	Pubmed	SVM Score :0.0
Genomic Southern analyses indicated that the members of the rat CYP11B gene subfamily were confined to these four genes ; among them , CYP11B1 and B 2 encoded steroid 11 beta hydroxylase and aldosterone synthase , respectively , while CYP11B3 was a gene highly homologous to CYP11B1 without a known expression product . ^^^
Interacting proteins: P15538 and P19099	Pubmed	SVM Score :0.0
Paramecium was found to have two closely related copine genes , CPN 1 and CPN 2 . ^^^
Interacting proteins: P15538 and P19099	Pubmed	SVM Score :0.0
Carboxypeptidase N ( CPN ) is a plasma zinc metalloprotease , which consists of two enzymatically active small subunits ( CPN 1 ) and two large subunits ( CPN 2 ) that protect the protein from degradation . ^^^
Interacting proteins: P15538 and P19099	Pubmed	SVM Score :0.0
Glucocorticoid suppressible hyperaldosteronism is a dominantly inherited form of hypertension believed to be caused by the presence of a hybrid CYP11B1 / CYP11B2 gene which has arisen from an unequal crossing over between the two CYP11B genes in a previous meiosis . ^^^ The hybrid CYP11B gene was demonstrated to be expressed at higher levels than either CYP11B1 or CYP11B2 in the cortex of the adrenal by RT PCR and Northern blot analysis . ^^^
Interacting proteins: P15538 and P19099	Pubmed	SVM Score :0.0
Unequal crossing over between the CYP11B genes can generate a duplicated chimeric gene with the transcriptional regulatory region of CYP11B1 but sufficient coding sequences from CYP11B2 so that the encoded enzyme has aldosterone synthase ( i . e . 18 oxidase ) activity . ^^^
Interacting proteins: P15538 and P19099	Pubmed	SVM Score :0.0
Three forms of rat CYP11B genes : 11 beta hydroxylase gene , aldosterone synthase gene , and a novel gene . ^^^
Interacting proteins: P15538 and P19099	Pubmed	SVM Score :0.0
These data suggest that neither 11beta hydroxylase nor aldosterone synthase are responsible for the biosynthesis of 18 OH B by ZF cells from DOC or 18 OH DOC , that 20 % of aldosterone synthesis appears not to be attributable to the actions of aldosterone synthase and that an unknown CYP11B enzyme is also involved in the biosynthesis of 18 OH B . . ^^^
Interacting proteins: P15538 and P19099	Pubmed	SVM Score :0.0
The last three steps of aldosterone biosynthesis , 11 beta hydroxylation , 18 hydroxylation and 18 oxidation , have been demonstrated to be catalysed by one enzyme , which is the cytochrome P 450 ( 11 beta ) ( CYP11B ) in cow , pig , sheep and bullfrog or cytochrome P450aldo ( CYP11B2 ) in rat , human , mouse and hamster . 2 . ^^^
Interacting proteins: P15538 and P19099	Pubmed	SVM Score :0.0
Transfection studies with cDNAs encoding hybrids between the highly similar cytochrome P 450 enzymes , CYP11B1 ( steroid 11 beta hydroxylase ) and CYP11B2 ( aldosterone synthase ) have identified which amino acids determine the different activities of the enzymes . . ^^^
Interacting proteins: P15538 and P19099	Pubmed	SVM Score :0.0
In this study , the ovine steroid 11 beta hydroxylase ( P 450 ( 11 beta ) or CYP11B ) cDNA previously reported by us ( 1 ) was transfected into COS 7 cells . ^^^
Interacting proteins: P15538 and P19099	Pubmed	SVM Score :0.0
INTRACELLULAR DISTRIBUTION AND PROPERTIES OF STEROID 11 BETA HYDROXYLASE AND STEROID 18 HYDROXYLASE IN RAT ADRENAL . ^^^
Interacting proteins: P15538 and P19099	Pubmed	SVM Score :0.0
Angiotensin 1 converting enzyme ( ACE ) and carboxypeptidase N 1 and N 2 ( CPN 1 , CPN 2 ) inactivate kinins and might therefore play a role in the development of inflammatory reactions via an influence on the release of prostaglandins and inactivation of anaphylatoxic peptides of the complement system . ^^^ CPN 1 and CPN 2 were raised in both diseases ( p less than 0 . 005 ) , irrespective of their activity and location . ^^^
Interacting proteins: P15538 and P19099	Pubmed	SVM Score :0.0
The last three steps of aldosterone biosynthesis have been demonstrated to be catalysed by a single enzyme , referred to as CYP11B ( or P 450 ( 11 ) beta ) in cow , pig , sheep and bullfrog and as CYP11B2 ( or P450aldo ) in rat , human , mouse and hamster . 2 . ^^^
Interacting proteins: P15538 and P19099	Pubmed	SVM Score :0.0
Their production by the recombinant CYP11B enzyme is enhanced by substitution of further amino acids encoded in exons 4 , 5 , and 6 of CYP11B2 . ^^^
Interacting proteins: P15538 and P19099	Pubmed	SVM Score :0.0
To investigate structure function relationships of CYP11B , wild type rat CYP11B1 and CYP11B2 and DR CYP11B1 ( mutant CYP11B1 in Dahl ' s salt resistant rats ) have been successfully expressed in Escherichia coli . ^^^
Interacting proteins: P15538 and P19099	Pubmed	SVM Score :0.0
The effect of Adx 1 108 on the product patterns of bovine CYP11B1 , human CYP11B1 and human CYP11B2 was confirmed in COS 1 cells by cotransfection of CYP11B and Adx containing expression vectors . ^^^
Interacting proteins: P15538 and P19099	Pubmed	SVM Score :0.0
The aim of this work was to evaluate whether other genetic alterations exist in CYP11B genes ( gene conversion in the coding region of CYP11B1 or in the promoter of CYP11B2 ) that could explain a positive dexamethasone suppression test and to determine another genetic cause of glucocorticoid remediable aldosteronism . ^^^
Interacting proteins: P15538 and P19099	Pubmed	SVM Score :0.0
We conclude that eNOS may play a role in controlling zone specific aldosterone synthase vs . 11 beta hydroxylase activities of the single CYP11B gene in sheep . ^^^
Interacting proteins: P15538 and P19099	Pubmed	SVM Score :0.0
The combination of functional data and computer modelling of CYP11B2 suggests an indirect involvement of residue 147 in the regulation of CYP11B isoform specific substrate conversion due to its location on the protein surface . ^^^
Interacting proteins: P15538 and P19099	Pubmed	SVM Score :0.0
Analyses of the CYP11B gene family in the guinea pig suggest the existence of a primordial CYP11B gene with aldosterone synthase activity . ^^^ These experiments suggest that a common CYP11B ancestor gene that possessed both 11beta hydroxylase and aldosterone synthase activity underwent a gene duplication event before or shortly after the mammalian radiation with subsequent independent evolution of the system in different lines . ^^^
Interacting proteins: P15538 and P19099	Pubmed	SVM Score :0.0
On molecular analysis , a homozygous deletion hybrid ( CYP11B2 CYP11B1 ) involving the CYP11B locus at 8q24 . 3 was found . ^^^
Interacting proteins: P15538 and P19099	Pubmed	SVM Score :0.0
They were tested for activity using bovine CYP11B and human CYP11B2 expressed in fission yeast and V 79 MZh cells . ^^^
Interacting proteins: P15538 and P19099	Pubmed	SVM Score :0.0
A recent report has indicated that GSH is caused by a gene duplication arising from unequal crossing over between the two genes , CYP11B1 and CYP11B2 , encoding P 450 ( 11 beta ) and P 450C18 , respectively ( Lifton et al . ^^^ Namely , the chimeric gene encodes a fused P 450 protein consisting of the amino terminal side of P 450 ( 11 beta ) ( encoded by exons 1 4 of CYP11B1 ) and the carboxyl terminal side of P 450C18 ( encoded by exons 5 9 of CYP11B2 ) . ^^^
Interacting proteins: P15538 and P19099	Pubmed	SVM Score :0.0
It is genetically linked to the CYP11B1 and CYP11B2 genes that , respectively , encode two cytochrome P 450 isozymes , P450XIB1 and P450XIB2 . ^^^ No mutations were found in the CYP11B1 genes , but two candidate mutations , R181W and V386A , were found in the CYP11B2 genes . ^^^
Interacting proteins: P15538 and P19099	Pubmed	SVM Score :0.0
In the human , mouse , and rat , two genes have been isolated , designated CYP11B1 and CYP11B2 . ^^^
Interacting proteins: P15538 and P19099	Pubmed	SVM Score :0.0
There are two distinct 11 beta hydroxylase genes in man , CYP11B1 and CYP11B2 , which are predicted to encode proteins with 93 % amino acid identity . ^^^ Expression of these cDNAs in cultured COS 1 cells demonstrated that the CYP11B1 product could only 11 beta hydroxylate 11 deoxycortisol or deoxycorticosterone , whereas the CYP11B2 product could also 18 hydroxylate cortisol or corticosterone . ^^^
Interacting proteins: P15538 and P19099	Pubmed	SVM Score :0.0
The genetic basis of the syndrome reflects the presence of a chimaeric gene derived from an unequal crossover between CYP11B1 and CYP11B2 , resulting in ACTH sensitive aldosterone synthase activity . ^^^
Interacting proteins: P15538 and P19099	Pubmed	SVM Score :0.0
The cDNAs of human 11 beta hydroxylase ( CYP11B1 ) and aldosterone synthase ( CYP11B2 ) were cloned from surgically removed normal human adrenal using polymerase chain reaction . ^^^ Cloning of CYP11B1 and CYP11B2 from normal human adrenal and their functional expression in COS 7 and V 79 Chinese hamster cells . ^^^ Stable expression of human CYP11B1 or CYP11B2 was performed in V 79 hamster cells and confirmed by Southern blotting , Northern blotting , and enzymatic activity . ^^^ Interestingly , recombinant V 79 cells were able to support CYP11B1 and CYP11B2 dependent steroid conversion without additional heterologous expression of the corresponding electron donor system . . ^^^
Interacting proteins: P15538 and P19099	Pubmed	SVM Score :0.0
Microsatellite polymorphism analysis allows the individual assignment of the rat 11 beta hydroxylase gene ( Cyp11b1 ) and the rat aldosterone synthase gene ( Cyp11b2 ) to chromosome 7 using rat 10 mouse somatic cell hybrids and identifies differences between and within various rat strains . ^^^ Mouse hepatoma 10 rat hepatocyte hybrids that segregate rat chromosomes were used to determine the chromosomal location of the rat genes encoding 11 beta hydroxylase and aldosterone synthase ( Cyp11b1 and Cyp11b2 respectively ) . ^^^
Interacting proteins: P15538 and P19099	Pubmed	SVM Score :0.0
Glucocorticoid suppressible hyperaldosteronism results from hybrid genes created by unequal crossovers between CYP11B1 and CYP11B2 . ^^^ A related isozyme in the zona fasciculata , CYP11B1 , is required for cortisol synthesis ; this isozyme , which is normally expressed at much higher levels than CYP11B2 , only has 11 beta hydroxylase activity . ^^^ The extra gene is a hybrid with 5 ' regulatory and coding regions corresponding to CYP11B1 and 3 ' coding regions from CYP11B2 . ^^^ Cells transfected with hybrid cDNAs containing up to the first three exons of CYP11B1 synthesized aldosterone at levels near that of cells carrying normal CYP11B2 , but cells transfected with hybrids containing the first five or more exons of CYP11B1 could not synthesize detectable amounts of aldosterone . ^^^
Interacting proteins: P15538 and P19099	Pubmed	SVM Score :0.0
CYP11B1 ( non aldosterone producing ) and CYP11B2 ( aldosterone producing ) . ^^^ According to this evidence , the expression of the two genes encoding the two isozymes ( CYP11B1 and CYP11B2 ) in rat zona glomerulosa cells is separately regulated . ^^^ Whereas CYP11B2 expression is controlled mainly by angiotensin 2 and extracellular potassium , ACTH is the major but not the only factor controlling CYP11B1 expression in these cells . . ^^^
Interacting proteins: P15538 and P19099	Pubmed	SVM Score :0.0
In principle , deletions of CYP11B1 could be generated by unequal crossing over between CYP11B1 and the adjacent CYP11B2 gene , but no such deletions were found among the deficiency alleles in this study . ^^^
Interacting proteins: P15538 and P19099	Pubmed	SVM Score :0.0
The cloned enzymes CYP11B1 and CYP11B2 would pertain respectively to the FIS and FS categories . . ^^^
Interacting proteins: P15538 and P19099	Pubmed	SVM Score :0.0
Stable expression of rat cytochrome P 450 11 beta hydroxylase ( CYP11B1 ) and aldosterone synthase ( CYP11B2 ) in MA 10 cells . ^^^ Glucocorticoids and mineralocorticoids are synthesized in the adrenal cortex through the action of two different cytochrome 11 beta hydroxylases , CYP11B1 ( 11 beta hydroxylase ) and CYP11B2 ( aldosterone synthase ) which are distributed in the zona fasciculata and glomerulosa , respectively . ^^^ We have created stably transfected cell lines using the Leydig tumor cell line MA 10 with CYP11B1 and CYP11B2 cDNA containing plasmids which have a selectable gene to confer resistance to geneticin . ^^^ CYP11B2 transfected cells ( but not the CYP11B1 transfected cells ) transform 18 OH DOC into 18 OH corticosterone , but can not convert 18 OH DOC into aldosterone . ^^^ In conclusion , stably transfected MA 10 cells with the cDNAs for the CYP11B1 and CYP11B2 enzymes were prepared and their enzymatic activity studied . ^^^
Interacting proteins: P15538 and P19099	Pubmed	SVM Score :0.0
CYP11B1 ( 11 beta hydroxylase ) and CYP11B2 ( aldosterone synthase ) are steroidogenic enzymes which mediate the final step ( 11 beta hydroxylation ) in cortisol synthesis and the final three steps ( 11 beta hydroxylation , 18 hydroxylation , and 18 oxidation ) in aldosterone synthesis , respectively . ^^^
Interacting proteins: P15538 and P19099	Pubmed	SVM Score :0.0
The hybrid rat cytochrome P 450 containing the first 5 exons of the CYP11B1 and last 4 exons from the CYP11B2 enzyme retains 11 beta hydroxylase activity , but the alternative hybrid is inactive . ^^^ In rat the coding nucleotide sequence and the deduced amino acid sequences of the CYP11B1 and CYP11B2 genes are homologous by 88 % and 83 % , respectively . ^^^ We have constructed two different hybrid cDNAs by exchanging two fragments of the rat CYP11B1 and CYP11B2 at the junction of the 5 / 6 exon and expressed them in COS 7 cells . ^^^
Interacting proteins: P15538 and P19099	Pubmed	SVM Score :0.0
In the rat adrenal cortex , two isozymes of cytochrome P 450 ( 11 ) beta ( CYP11B1 and CYP11B2 ) have been identified . ^^^ In vivo and in vitro , the expression of the genes encoding CYP11B1 and CYP11B2 is regulated by two separate control systems which appear to operate both independently and interdependently . ^^^
Interacting proteins: P15538 and P19099	Pubmed	SVM Score :0.0
The effects of the extracellular potassium concentration on the expression of four steroidogenic cytochromes P 450 ( CYP11B2 , CYP11B1 , CYP11A1 , and CYP21A1 ) in cultured rat adrenal zona glomerulosa cells were studied by Northern blot analysis . ^^^ In zona fasciculata cells , CYP11B2 expression was not inducible by potassium ; CYP11B1 , CYP11A1 , and CYP21A1 mRNA levels increased in response to ACTH , but not in response to a high potassium concentration . ^^^
Interacting proteins: P15538 and P19099	Pubmed	SVM Score :0.0
The biosynthesis of glucocorticoids and mineralocorticoids in the rat adrenal cortex requires the action of two different cytochrome P 450 11 beta hydroxylases , CYP11B1 and CYP11B2 , which are distributed in the zona fasciculata and glomerulosa , respectively . ^^^
Interacting proteins: P15538 and P19099	Pubmed	SVM Score :0.0
P 45011 beta ( CYP11B1 ) and P450aldo ( CYP11B2 ) are expressed in rat brain , but not in mouse brain ; CYP11B1 primarily in the cerebrum , whereas CYP11B2 was detected in all brain regions . ^^^
Interacting proteins: P15538 and P19099	Pubmed	SVM Score :0.0
In this study we examined the effects of angiotensin 2 ( ANG 2 ) , potassium , and ACTH on the production of aldosterone , the activity of aldosterone synthase , and the expression of CYP11B2 and CYP11B1 messenger ribonucleic acid ( mRNA ) in cultured human vascular endothelial cells . ^^^ The levels of CYP11B2 and CYP11B1 mRNA were determined by competitive PCR . ^^^ Both ANG 2 and potassium increased the concentration of CYP11B2 mRNA , but not that of CYP11B1 mRNA . ^^^
Interacting proteins: P15538 and P19099	Pubmed	SVM Score :0.0
The effects of a calcium channel blocker ( nifedipine ) and a protein synthesis inhibitor ( cycloheximide ) on the induction of four steroidogenic cytochromes P 450 ( CYP11B2 , CYP11B1 , CYP11A1 , and CYP21A1 ) by an elevated extracellular potassium concentration were studied in cultured rat zona glomerulosa cells . ^^^
Interacting proteins: P15538 and P19099	Pubmed	SVM Score :0.0
It is caused by recombinations between linked genes encoding closely related isozymes , 11 beta hydroxylase ( CYP11B1 ) and aldosterone synthase ( CYP11B2 ) , generating a dysregulated chimeric gene with aldosterone synthase activity . ^^^
Interacting proteins: P15538 and P19099	Pubmed	SVM Score :0.0
Changed ratios of glucocorticoids / mineralocorticoids caused by point mutations in the putative 1 helix regions of CYP11B1 and CYP11B2 . ^^^ Computer modelling and site directed mutagenesis were employed to investigate the structural basis for the regioselectivity of steroid hydroxylation and to determine whether point mutations of CYP11B1 and CYP11B2 can result in changes in the ratio of glucocorticoids / mineralocorticoids . ^^^ Single replacement of CYP11B2 residues for CYP11B1 specific residues at positions 296 , 301 , 302 , 320 , and 335 , belonging to the putative 1 helix , gave rise to slightly increased 11 beta hydroxylase activities . ^^^ Replacement of 3 amino acids of CYP11B2 by the corresponding residues in CYP11B1 was sufficient to increase cortisol formation from about 5 % to 85 % of the CYP11B1 wild type level . ^^^ Replacement of Val 320 of CYP11B1 by alanine , the corresponding residue found in CYP11B2 , led to production of aldosterone by this mutant enzyme . ^^^
Interacting proteins: P15538 and P19099	Pubmed	SVM Score :0.0
Regulation of aldosterone synthase cytochrome P 450 ( CYP11B2 ) and 11 beta hydroxylase cytochrome P 450 ( CYP11B1 ) expression in rat adrenal zona glomerulosa cells by low sodium diet and angiotensin 2 receptor antagonists . ^^^ Changes in the mRNA levels for aldosterone synthase cytochrome P 450 ( CYP11B2 or P450aldo ) and 11 beta hydroxylase cytochrome P 450 ( CYP11B1 or P 45011 beta ) in rat adrenal glands were studied in response to angiotensin 2 type 1 ( AT 1 ) and type 2 ( AT 2 ) receptor antagonists . ^^^ CYP11B1 and CYP11B2 genes were highly homologous ( 88 . 5 % ) in their nucleotide sequences of the amino acid coding regions . ^^^ Reverse transcription polymerase chain reactions ( RT PCR ) which are capable of discriminating between rat CYP11B1 and CYP11B2 , were performed with specific primers for each P 450 . ^^^ Upon sodium restriction ( 5 mmol Na ( + ) / kg of diet ) of rats for 14d , the amount of the CYP11B2 mRNA in the adrenal glands was increased 8 . 5 fold compared to that from the rats fed a normal diet ( 225 mmol Na ( + ) / kg of diet ) , whereas no significant change in the CYP11B1 mRNA was observed after the dietary sodium restriction . ^^^
Interacting proteins: P15538 and P19099	Pubmed	SVM Score :0.0
Expression of 11 beta hydroxylase ( CYP11B1 ) and aldosterone synthase ( CYP11B2 ) in the human fetal adrenal . ^^^ OBJECTIVE : To understand better the steroidogenic capacity of the human fetal adrenal ( HFA ) , we evaluated the expression of 11 beta hydroxylase ( CYP11B1 ) and aldosterone synthase ( CYP11B2 ) in the fetal zone and neocortex of the HFA using a specific RNase protection assay . ^^^ RNase protection assays were then performed using radiolabeled complementary RNA probes generated by T 7 RNA polymerase directed against transcripts for CYP11B1 , CYP11B2 , and actin , the latter of which was used as a control for RNA integrity . ^^^ Transcripts also were examined using a reverse transcription polymerase chain reaction ( RT PCR ) protocol specific for CYP11B1 or CYP11B2 . ^^^ RESULTS : The RNase protection assay was designed to distinguish specific bands that corresponded to CYP11B1 ( 232 bp ) , CYP11B2 ( 262 bp ) , and actin ( 221 bp ) . ^^^
Interacting proteins: P15538 and P19099	Pubmed	SVM Score :0.0
Since 11 beta hydroxylase P 450 ( P 45011 beta or CYP11B1 ) , which shows high homology ( 88 . 5 % ) with P450aldo in their nucleotide sequences of the amino acid coding regions , is also expressed in the adrenal gland , RT PCR was performed with specific primers for each P 450 . ^^^
Interacting proteins: P15538 and P19099	Pubmed	SVM Score :0.0
Two of these polymorphisms in CYP11B1 represent sequences from CYP11B2 , suggesting that gene conversion may have occurred . ^^^
Interacting proteins: P15538 and P19099	Pubmed	SVM Score :0.0
Conferring aldosterone synthesis to human CYP11B1 by replacing key amino acid residues with CYP11B2 specific ones . ^^^ Performing residue swapping experiments between the highly conserved human steroidogenic proteins CYP11B1 and CYP11B2 we recently demonstrated that replacement of specific residues at position 301 , 302 and 320 in the aldosterone producing CYP11B2 protein for such residues that were specific for the highly similar cortisol producing CYP11B1 protein elevated the 11beta hydroxylase activity dramatically . ^^^ Here we provide evidence that in a reciprocal experiment , CYP11B2 specific amino acids at position 320 and 335 endowed CYP11B1 with an 18 oxidase function amounting to 20 % of the CYP11B2 wild type activity , thus changing the specificity of steroid hydroxylation by only one point mutation . ^^^ Paradoxically , 11beta hydroxylation was not or only marginally affected in CYP11B1 mutants , indicating an alternative structural basis for this activity in CYP11B1 compared with the engineered CYP11B2 variant . ^^^ Our results suggest that the sequence spanned by amino acids 301 and 335 constitutes part of the substrate binding site in CYP11B1 and CYP11B2 as well . ^^^
Interacting proteins: P15538 and P19099	Pubmed	SVM Score :0.0
Anomalies in either of the tightly linked genes encoding the enzymes CYP11B1 ( 11beta hydroxylase ) or CYP11B2 ( aldosterone synthase ) can lead to important changes in arterial pressure and are responsible for several monogenically inherited forms of hypertension . ^^^ To test this hypothesis , we performed 2 complementary studies of the CYP11B1 / CYP11B2 locus in essential hypertension . ^^^
Interacting proteins: P15538 and P19099	Pubmed	SVM Score :0.0
The finding of easily demonstrable , intragenic variants will be beneficial to the study of the role of the CYP11B1 and the adjacent aldosterone synthase ( CYP11B2 ) gene in hypertensive disease . . ^^^
Interacting proteins: P15538 and P19099	Pubmed	SVM Score :0.0
The genes encoding aldosterone synthase ( CYP11B2 ) and 11 beta hydroxylase ( CYP11B1 ) are very similar at the nucleotide level ( > 95 % homology ) . ^^^
Interacting proteins: P15538 and P19099	Pubmed	SVM Score :0.0
In species such as the human , rat and mouse , the conversion of deoxycorticosterone to aldosterone is catalysed by an isozyme ( CYP11B2 ) of cytochrome P 450 ( 11 ) beta ( CYP11B1 ) . ^^^
Interacting proteins: P15538 and P19099	Pubmed	SVM Score :0.0
Zonal function in turn requires differential expression of CYP17 / 3betaHSD and CYP11B2 / CYP11B1 . ^^^ In turn both K+ and AII induced elevation of Ca2+ strongly induces CYP11B2 but not CYP11B1 , consistent with preferential expression of CYP11B2 in the zg . ^^^
Interacting proteins: P15538 and P19099	Pubmed	SVM Score :0.0
Amino acid residues in 1 and K helices of rat CYP11B1 and CYP11B2 are important in expression of 18 hydroxylation activity . ^^^ When a peptide of the 316 398th amino acid residues in CYP11B1 was inserted to the corresponding site in CYP11B2 , the 18 hydroxylation activity of the chimera decreased to a level similar to that of CYP11B2 . ^^^ Conversely , when the corresponding region of CYP11B2 was changed to that of CYP11B1 , the 18 hydroxylation activity increased to the similar level as that of CYP11B1 . ^^^
Interacting proteins: P15538 and P19099	Pubmed	SVM Score :0.0
The adrenal 11 beta hydroxylase is a mitochondrial P 450 enzyme encoded by the CYP11B1 gene , which is situated on chromosome 8q22 in tandem with the gene for aldosterone synthase ( CYP11B2 ) . ^^^
Interacting proteins: P15538 and P19099	Pubmed	SVM Score :0.0
The diagnosis was confirmed by genetic testing , in the index case and in one of family members , which demonstrated the chimeric gene duplication , which was a resultant of a crossing over between the proximal portion of 11 beta hydroxylase gen , CYP11B1 , and the distal portion of aldosterone synthetase gene CYP11B2 . ^^^
Interacting proteins: P15538 and P19099	Pubmed	SVM Score :0.0
Vascular endothelial and smooth muscle cells express CYP11B1 and CYP11B2 , genes responsible for 11 beta hydroxylase and aldosterone synthase , respectively . ^^^
Interacting proteins: P15538 and P19099	Pubmed	SVM Score :0.0
The capacity of the adrenal cortex to differentially produce aldosterone and cortisol relies to a large degree on the expression of aldosterone synthase ( CYP11B2 ) and 11beta hydroxylase ( CYP11B1 ) . ^^^ CYP11B2 catalyzes the final steps in the biosynthesis of aldosterone and is expressed solely in the glomerulosa of the adrenal cortex , while CYP11B1 catalyzes the final steps in the biosynthesis of cortisol and is expressed in the fasciculata / reticularis . ^^^
Interacting proteins: P15538 and P19099	Pubmed	SVM Score :0.0
It was shown that when human or bovine CYP11B1 and CYP11A1 were cotransfected they competed for the reducing equivalents from the limiting source contained in COS 1 cells ; this resulted in a decrease of the CYP11B activities without changes in the product formation patterns . ^^^ The interactions of CYP11B1 ( cytochrome P 45011beta ) , CYP11B2 ( cytochrome P 450aldo ) and CYP11A1 ( cytochrome P 450scc ) were investigated by cotransfection of their cDNA into COS 1 cells . ^^^ The competition of human CYP11A1 with human CYP11B1 and CYP11B2 could be diminished with excess expression of bovine adrenodoxin . ^^^ These results suggest that the interactions of CYP11A1 with CYP11B1 and CYP11B2 do not have an identical regulatory function in human and in bovine adrenal tissue . . ^^^
Interacting proteins: P15538 and P19099	Pubmed	SVM Score :0.0
Molecular genetic studies of the CYP11B1 and CYP11B2 genes in 11beta hydroxylase deficiency or aldosterone synthase deficiency have led to the identification of several mutations . ^^^ The substrate for CYP11B2 is 11 deoxycorticosterone , that of CYP11B1 is 11 deoxycortisol . ^^^
Interacting proteins: P15538 and P19099	Pubmed	SVM Score :0.0
In the human adrenal cortex , cortisol and aldosterone are synthesized by the isozymes 11beta hydroxylase and aldosterone synthase , respectively , encoded by the 93 % identical CYP11B1 and CYP11B2 genes . ^^^ In vitro mutagenesis of CYP11B1 complementary DNA , resulting in the replacement of CYP11B1 codons by those encoding the corresponding amino acid residues of CYP11B2 enzyme ( exon 5 , Ser288Gly ; exon 6 , Val320Ala ) , yields a complementary DNA encoding a mutant enzyme with an efficient aldosterone synthase activity . ^^^
Interacting proteins: P15538 and P19099	Pubmed	SVM Score :0.0
The terminal stages of cortisol and aldosterone production in the human adrenal gland are catalysed by the enzymes 11beta hydroxylase and aldosterone synthase , which are encoded by the CYP11B1 and CYP11B2 genes respectively . ^^^ Reverse transcription polymerase chain reaction ( RT PCR ) / Southern blotting confirmed transcription of CYP11B1 and CYP11B2 in whole brain and hypothalamus minces from Wistar Kyoto rats . 11beta Hydroxylase and aldosterone synthase were immunolocalised in paraffin embedded rat adrenal and brain sections using mouse monoclonal antibodies . ^^^
Interacting proteins: P15538 and P19099	Pubmed	SVM Score :0.0
This autosomal dominant disorder has been shown to be caused by a hybrid gene mutation formed by a crossover of genetic material between the ACTH responsive regulatory portion of the 11ss hydroxylase ( CYP11B1 ) gene and the coding region of the aldosterone synthase ( CYP11B2 ) gene . ^^^
Interacting proteins: P15538 and P19099	Pubmed	SVM Score :0.0
Unlike FH type 1 ( FH 1 ) , which results from fusion of the CYP11B1 and CYP11B2 genes , hyperaldosteronism in FH 2 is not glucocorticoid remediable . ^^^
Interacting proteins: P15538 and P19099	Pubmed	SVM Score :0.0
Genetic investigations of mendelian hypertension has identified the genetic mechanisms for glucocorticoid remediable aldosteronism , apparent mineral corticoid excess , and Liddle ' s syndrome as chimeric gene duplications of CYP11B1 ( aldosterone synthase gene ) and CYP11B2 ( 11beta hydroxylase gene ) , mutations in the gene of 11beta hydroxysteroid dehydrogenase type 2 that catalyzes the conversion of cortisol to cortisone , and mutations in beta or gamma subunit of epithelial sodium channel ( ENaC ) , respectively . ^^^
Interacting proteins: P15538 and P19099	Pubmed	SVM Score :0.0
The regional expression of these isozymes is believed to result from transcriptional regulation of the aldosterone synthase ( CYP11B2 ) and 11beta hydroxylase ( CYP11B1 ) genes . ^^^ Regulation of human CYP11B2 and CYP11B1 : comparing the role of the common CRE / Ad1 element . ^^^
Interacting proteins: P15538 and P19099	Pubmed	SVM Score :0.0
This small segment still contained the primary candidate locus Cyp11b1 ( 11beta hydroxylase ) , but the adjacent candidate genes Cyp11b2 ( aldosterone synthase ) and Cyp11b3 were ruled out . ^^^
Interacting proteins: P15538 and P19099	Pubmed	SVM Score :0.0
A gene conversion between the CYP11B1 and CYP11B2 genes could be responsible for such a substitution . ^^^
Interacting proteins: P15538 and P19099	Pubmed	SVM Score :0.0
The gene ( CYP11B1 ) coding for this enzyme is highly homologous with and lies a relatively short distance downstream from the gene coding for aldosterone synthase ( CYP11B2 ) on chromosome 8 . ^^^ Thus , variation in CYP11B2 appears to affect the product of CYP11B1 . ^^^
Interacting proteins: P15538 and P19099	Pubmed	SVM Score :0.0
Here we describe the first case of 11beta hydroxylase deficiency that is caused by an unequal cross over of the genes encoding aldosterone synthase ( CYP11B2 ) and 11beta hydroxylase ( CYP11B1 ) . ^^^ CYP11B1 and CYP11B2 are located on chromosome 8q24 approximately 45 kb apart from each other . ^^^ The existence of the CYP11B2 / CYP11B1 chimera was discovered by means of a PCR method and was confirmed with a Southern blot . ^^^
Interacting proteins: P15538 and P19099	Pubmed	SVM Score :0.0
Oligonucleotide probes with sequences complementary to mRNAs encoding for the steroid synthesizing enzymes 11 beta hydroxylase ( CYP11B1 ) , 18 hydroxylase ( CYP11B2 ) , 17 alpha hydroxylase ( CYP 17 ) , and 21 hydroxylase ( CYP 21 ) were synthesized ( Genset , Paris , France ) and in situ hybridization was performed . ^^^ Moderate expression of CYP11B2 and low expression of CYP11B1 were seen in the zona glomerulosa . ^^^ The zona fasciculata of the control adrenals expressed a high signal of CYP11B1 , whereas the expression of CYP11B2 was very low . ^^^ The two patients with highest release of aldosterone in vitro showed the highest expression of CYP11B2 and the lowest expression of CYP11B1 and CYP 17 . ^^^
Interacting proteins: P15538 and P19099	Pubmed	SVM Score :0.0
The chimeric CYP11B1 / CYP11B2 gene , which is a candidate gene for glucocorticoid remediable hyperaldosteronism , was not found in either the DNA from aldosteronoma or in the genomic DNA from patients with APA or IHA . ^^^
Interacting proteins: P15538 and P19099	Pubmed	SVM Score :0.0
RNA was reverse transcribed and probed with specific primers for side chain cleavage enzyme ( CYP11A ) , 3beta hydroxysteroid dehydrogenase , aldosterone synthase ( CYP11B2 ) , 11beta hydroxylase ( CYP11B1 ) , steroidogenic factor 1 , and steroid acute regulatory protein . ^^^ In failing human heart , CYP11B1 and CYP11B2 were detected in some samples , in contrast with normal hearts , which expressed neither ; in the mouse heart steroidogenic factor 1 was detected , but neither CYP11B1 nor CYP11B2 was found . ^^^
Interacting proteins: P15538 and P19099	Pubmed	SVM Score :0.0
Although a chimeric gene combining the 11beta hydroxylase gene ( CYP11B1 ) and the aldosterone synthase gene ( CYP11B2 ) explains the pathophysiology of familial hyperaldosteronism ( FH ) type 1 , the contribution of this abnormality to FH type 2 has not been tested . ^^^ A chimeric CYP11B1 / CYP11B2 gene in glucocorticoid insuppressible familial hyperaldosteronism . ^^^ We screened genomic DNA from a Japanese family with FH type 2 for the CYP11B1 / CYP11B2 gene . ^^^ Samples were amplified by polymerase chain reaction using specific primers from CYP11B1 and CYP11B2 . ^^^ The CYP11B1 / CYP11B2 chimeric gene was found in a Japanese family with FH type II . . ^^^
Interacting proteins: P15538 and P19099	Pubmed	SVM Score :0.0
An inherited form of primary hyperaldosteronism is the glucocorticoid remediable aldosteronism ( GRA ) caused by an unequal crossing over between the CYP11B1 and CYP11B2 genes that results in a chimeric gene , which has aldosterone synthase activity regulated by ACTH . ^^^
Interacting proteins: P15538 and P19099	Pubmed	SVM Score :0.0
The effects of these compounds and also of 18 hydroxydeoxycorticosterone were tested in cells stably transfected with CYP11B1 and CYP11B2 , the genes encoding 11beta hydroxylase and aldosterone synthase , respectively . ^^^
Interacting proteins: P15538 and P19099	Pubmed	SVM Score :0.0
To examine the effects of LRH on steroidogenic capacity we used reporter constructs prepared with the 5 ' flanking region of steroidogenic acute regulatory protein ( StAR ) , cholesterol side chain cleavage ( CYP11A1 ) , 3beta hydroxysteroid dehydrogenase type 2 ( HSD3B2 ) , 17alpha hydroxylase , 17 , 20 lyase ( CYP 17 ) , 11beta hydroxylase ( CYP11B1 ) and aldosterone synthase ( CYP11B2 ) . ^^^
Interacting proteins: P15538 and P19099	Pubmed	SVM Score :0.0
One polymorphism affects a putative steroidogenic factor 1 binding site ( 344 T / C ) in the 5 ' regulatory region , whereas the other marker reflects replacement of the intron 2 from CYP11B2 with that from the neighboring gene encoding 11beta hydroxylase ( CYP11B1 ; wild type / conversion ) . ^^^
Interacting proteins: P15538 and P19099	Pubmed	SVM Score :0.0
The final steps of the biosynthesis of glucocorticoids and mineralocorticoids in the adrenal cortex require the action of two different cytochromes P 450 CYP11B1 and CYP11B2 . ^^^ Principal attention was given to the modelling of the active sites and a comparison of the active site structures of CYP11B1 and CYP11B2 was performed . ^^^ The obtained 3D structures of CYP11B1 and CYP11B2 were used for investigation of structure function relationships of these enzymes . ^^^
Interacting proteins: P15538 and P19099	Pubmed	SVM Score :0.0
Genes encoding aldosterone synthase ( CYP11B2 ) and 11beta hydroxylase ( CYP11B1 ) in MHS and MNS have been cloned and sequenced . ^^^ Nucleotide 752 ( G ) in exon 4 of MHS CYP11B2 differs from that of MNS ( A ) ; CYP11B1 sequences were identical . ^^^
Interacting proteins: P15538 and P19099	Pubmed	SVM Score :0.0
The aim of the present study was to determine whether CYP11B2 and CYP11B1 ( 11beta hydroxylase ) are expressed in myocardial tissues , and whether these enzymes contribute to collagen accumulation and myocardial dysfunction in the failing human heart . ^^^ CYP11B2 and CYP11B1 mRNA levels were measured by real time quantitative reverse transcriptase PCR . ^^^ CYP11B2 mRNA expression was greater in the CHF group than in the controls ( P < 0 . 05 ) , while CYP11B1 mRNA was barely expressed in either group . ^^^
Interacting proteins: P15538 and P19099	Pubmed	SVM Score :0.0
Glucocorticoid remediable hyperaldosteronism ( GRA ) is a monogenic form of inherited hypertension caused by a chimeric gene originating from an unequal cross over between the 11 beta hydroxylase ( CYP11B1 ) and aldosterone synthase ( CYP11B2 ) genes . ^^^ We investigated whether the cross over site ( between the CYP11B1 and CYP11B2 genes ) or biochemical characteristics could explain this phenotype . ^^^
Interacting proteins: P15538 and P19099	Pubmed	SVM Score :0.0
Human 11beta hydroxylase ( CYP11B1 ) , which catalyzes the production of glucocorticoids , shows more than 90 % homology compared to human CYP11B2 . ^^^ For that purpose , an assay procedure with V79MZ cells that express human CYP11B1 and CYP11B2 , respectively , was integrated into the evaluation process . ^^^
Interacting proteins: P15538 and P19099	Pubmed	SVM Score :0.0
By DNA sequence analysis , we found that the breakpoint of `` unequal crossing over ' ' is both within intron 2 of the 11beta hydroxylase gene ( CYP11B1 ) and the aldosterone synthase gene ( CYP11B2 ) . ^^^
Interacting proteins: P15538 and P19099	Pubmed	SVM Score :0.0
This autosomal dominant disorder has been shown to be caused by a hybrid gene mutation formed by a cross over of genetic material between the ACTH responsive regulatory portion of the 11b hydroxylase ( CYP11B1 ) gene and the coding region of the aldosterone synthase ( CYP11B2 ) gene . ^^^
Interacting proteins: P15538 and P19099	Pubmed	SVM Score :0.0
Two key players in these pathways are the human mitochondrial cytochrome P 450 enzymes CYP11B1 and CYP11B2 , which catalyze the final steps in the biosynthesis of cortisol and aldosterone . ^^^
Interacting proteins: P15538 and P19099	Pubmed	SVM Score :0.0
METHODS : In order to specifically quantify CYP11B1 , CYP11B2 ( aldosterone synthase ) and CYP 17 ( 17alpha hydroxylase ) mRNA levels , we developed a real time RT PCR assay and examined the expression in a series of adrenal tIssues , including six normal adrenals from patients adrenalectomized for renal cancer and twelve aldosterone producing adenomas ( APA ) from patients with primary aldosteronism . ^^^ Quantitative assessment of CYP11B1 and CYP11B2 expression in aldosterone producing adenomas . ^^^ In patients with APA , CYP11B2 and CYP11B1 mRNA levels were not correlated either with basal aldosterone or with the change from basal aldosterone in response to posture or to dexamethasone . ^^^ No correlation between CYP11B1 mRNA or CYP11B2 mRNA and the percentage of zona fasciculata like cells was observed in APA . ^^^ CONCLUSIONS : Real time RT PCR can be reliably used to quantify CYP11B1 and CYP11B2 mRNA levels in adrenal tIssues . ^^^
Interacting proteins: P15538 and P19099	Pubmed	SVM Score :0.0
We observed that TGFbeta 1 strongly inhibits forskolin induced steroid 11beta hydroxylase activity and CYP11B1 mRNA levels , as well as angiotensin 2 induced aldosterone synthase activity and CYP11B2 mRNA levels . ^^^ Transforming growth factor beta 1 inhibits aldosterone and cortisol production in the human adrenocortical cell line NCI H295R through inhibition of CYP11B1 and CYP11B2 expression . ^^^ CYP11B1 and CYP11B2 gene products thus appear as the major steroidogenic enzymes down regulated by TGFbeta 1 in the human adrenocortical tumor cell line NCI H295R . . ^^^
Interacting proteins: P15538 and P19099	Pubmed	SVM Score :0.0
The gene CYP11B2 encodes the steroid synthesizing enzymes for aldosterone production , while the genes CYP 17 and CYP11B1 are needed for cortisol production . ^^^
Interacting proteins: P15538 and P19099	Pubmed	SVM Score :0.0
We have developed a highly sensitive QRT PCR method for the measurement of CYP11B1 ( 11beta hydroxylase ) and CYP11B2 ( aldosterone synthase ) mRNAs to study their expression in the rat brain in response to dietary sodium manipulation and angiotensin ( Ang ) 2 infusion . ^^^ CYP11B2 and CYP11B1 expression was measured in RNA from adrenal gland and discrete brain regions using real time QRT PCR . ^^^ Brain CYP11B1 mRNA levels were 10 to 1000 fold higher than CYP11B2 but were unaffected by dietary sodium or AngII . ^^^
Interacting proteins: P15538 and P19099	Pubmed	SVM Score :0.0
Promoter analyses suggested that SIK repressed gene expressions not only of CYP11A and StAR but also of CYP11B1 , CYP11B2 and SIK itself . ^^^
Interacting proteins: P15538 and P19099	Pubmed	SVM Score :0.0
To test this hypothesis , real time reverse transcription polymerase chain reaction ( RT RTPCR ) assays were developed that quantify mRNA levels for steroidogenic acute regulatory protein ( StAR ) , cholesterol side chain cleavage ( CYP11A ) , 3beta hydroxysteroid dehydrogenase types 1 and 2 ( HSD3B1 and HSD3B2 ) , 17alpha hydroxylase ( CYP 17 ) , 21 hydroxylase ( CYP 21 ) , 11beta hydroxylase ( CYP11B1 ) , aldosterone synthase ( CYP11B2 ) and aromatase ( CYP 19 ) . ^^^
Interacting proteins: P15538 and P19099	Pubmed	SVM Score :0.0
The presence of a chimaeric CYP11B1 / CYP11B2 gene was demonstrated by long PCR and Southern blotting ( crossover site at the end of intron 3 ) in the proband , in the younger sister ( sibling 1 ) and in the father . ^^^ In these patients , sequencing of the chimaeric portion of CYP11B1 did not reveal any mutation , while sequencing of the chimaeric portion of CYP11B2 showed a V386A polymorphism in exon 7 , known to cause only a minimal impairment of enzymatic activity . ^^^
Interacting proteins: P15538 and P19099	Pubmed	SVM Score :0.0
CYP11B1 and the closely related CYP11B2 are involved in the production of adrenal steroid hormones . ^^^
Interacting proteins: P15538 and P19099	Pubmed	SVM Score :0.0
We have identified three genetic variants of CYP11B2 , 344C / T at the promoter region , a conversion in intron 2 from the CYP11B2 sequence to the CYP11B1 sequence , and R173K in exon 3 , in the Japanese population . 344C / T and R173K were in complete linkage disequilibrium in CYP11B2 , and 344C / T was in strong , but not complete , linkage disequilibrium with the polymorphism in intron 2 . ^^^
Interacting proteins: P15538 and P19099	Pubmed	SVM Score :0.0
BACKGROUND : In patients with glucocorticoid remediable aldosteronism ( GRA ) , a rare hypertensive disorder caused by the presence of a chimeric aldosterone synthase ( CYP11B2 ) and 11beta hydroxylase ( CYP11B1 ) gene , high level of urinary 18 hydroxycortisol ( 18OHF ) excretion are observed . ^^^ Mutation analysis of CYP11B1 and CYP11B2 in patients with increased 18 hydroxycortisol production . ^^^ In some patients with hypertension , increased urinary 18OHF secretion is also found in the absence of the hybrid CYP11B1 / CYP11B2 gene . ^^^ We hypothesised that gene variants of CYP11B1 or CYP11B2 may be linked to this abnormal glucocorticoid production . ^^^ All were tested negative for the hybrid CYP11B1 / CYP11B2 gene and were further analysed for mutations in all exons and promoter regions of both CYP11B1 and CYP11B2 by single strand conformation polymorphism ( SSCP ) and sequencing when appropriate . ^^^
Interacting proteins: P15538 and P19099	Pubmed	SVM Score :0.0
Here , we review the genetic and the phenotypic features of the familial forms , the genetic variants that influence the sporadic forms and the structure and regulation of the CYP11B1 and CYP11B2 genes . . ^^^
Interacting proteins: P15538 and P19099	Pubmed	SVM Score :0.0
In vitro , the CYP11B2 ( aldosterone synthase ) of the glomerulosa can and does utilise as substrates products arising from CYP11B1 ( 11beta hydroxylase ) activity in fasciculata cells . ^^^
Interacting proteins: P15538 and P19099	Pubmed	SVM Score :0.0
Raised plasma and urinary levels of 11 deoxycortisol suggest that there is relative inefficiency of 11beta hydroxylation in the zona fasciculata ; the P 450 enzyme responsible for this step is encoded by the gene CYP11B1 , which is highly homologous with and adjacent to CYP11B2 . ^^^
Interacting proteins: P15538 and P19099	Pubmed	SVM Score :0.0
After earlier studies in which secretion of aldosterone was demonstrated to be important in rat arterial smooth muscle cell ( RASMC ) proliferation in vitro , the presence of both 11beta hydroxylase ( CYP11B1 ) and aldosterone synthase ( CYP11B2 ) gene transcription were shown in these cells by real time reverse transcription polymerase chain reaction ( RT PCR ) . ^^^
Interacting proteins: P15538 and P19099	Pubmed	SVM Score :0.0
The hybrid gene is the product of fusion between the ACTH responsive regulatory portion of the 11b hydroxylase gene ( CYP11B1 ) and the coding region of the aldosterone synthase gene ( CYP11B2 ) . ^^^ This autosomal dominant disorder is caused by unequal cross over between two genes with wide sequence homology : CYP11B1 and CYP11B2 . ^^^
Interacting proteins: P15538 and P19099	Pubmed	SVM Score :0.0
Stably transfected cell lines expressing either CYP11B1 ( 11beta hydroxylase ) or CYP11B2 ( AS ) were incubated with cortisol and other substrates over a range of concentrations . ^^^
Interacting proteins: P15538 and P19099	Pubmed	SVM Score :0.0
CYP11B2 CYP11B1 haplotypes associated with decreased 11 beta hydroxylase activity . ^^^ The 11 beta hydroxylase gene ( CYP11B1 ) lies close to CYP11B2 . ^^^ We hypothesize that a molecular variant in CYP11B2 is in linkage disequilibrium ( LD ) with a key quantitative trait in CYP11B1 determining this phenotype . ^^^
Interacting proteins: P15538 and P19099	Pubmed	SVM Score :0.0
However , in normal subjects , aldosterone synthase does not metabolize S , which is converted to cortisol ( F ) by the enzyme 11 beta hydroxylase , encoded by the gene CYP11B1 , which lies adjacent to CYP11B2 on chromosome 8 . ^^^ We genotyped six polymorphisms in the CYP11B2 gene and three polymorphisms in the CYP11B1 gene in 248 Caucasian nuclear families comprising 1428 individuals . ^^^ There was strong evidence for association between polymorphisms of both CYP11B1 and CYP11B2 and urinary THS , which was strongest for the CYP11B1 exon 1 polymorphism ( P = 0 . 00002 ) . ^^^ Thus , it is likely that linkage disequilibrium between causative CYP11B1 variants and CYP11B2 polymorphisms account for the previous observations . ^^^
Interacting proteins: P15538 and P19099	Pubmed	SVM Score :0.0
Urine samples were obtained for gas chromatography mass spectroscopic measurements of steroid hormones reflecting apparent Aldo synthase ( CYP11B2 ) and 11 hydroxylase ( CYP11B1 ) activities . ^^^ RESULTS : CYP11B1 activity was unaltered , but reduction of mean tetrahydro ( TH ) Aldo excretion by a factor of 3 . 9 indicated a diminished CYP11B2 activity in preeclampsia . ^^^
Interacting proteins: P15538 and P19099	Pubmed	SVM Score :0.0
Development of test systems for the discovery of selective human aldosterone synthase ( CYP11B2 ) and 11beta hydroxylase ( CYP11B1 ) inhibitors . ^^^ Two key players in adrenal steroid hormone biosynthesis are the human mitochondrial cytochrome P 450 enzymes CYP11B1 and CYP11B2 that catalyze the final steps in the biosynthesis of cortisol and aldosterone , respectively . ^^^ Thus , CYP11B1 and CYP11B2 comprise new targets for drug treatment and selective inhibitors of both enzymes are of high pharmacological interest . ^^^ Using these test systems , we were able to identify a new and very selective CYP11B2 inhibitor ( SIAS 1 ) that displayed no detectable interference with CYP11B1 activity . . ^^^
Interacting proteins: P15538 and P19099	Pubmed	SVM Score :0.0
We previously have demonstrated that this yeast is a unique host for recombinant expression of human CYP11B2 ( aldosterone synthase ) , and here we report the functional production of human CYP11B1 ( steroid 11beta hydroxylase ) in S . pombe using our new integration vector pCAD 1 . ^^^
Interacting proteins: P15538 and P19099	Pubmed	SVM Score :0.0
Because it shares 95 % sequence homology with aldosterone synthetase ( CYP11B2 ) , we developed gene specific primers for differential PCR amplification of the CYP11B1 gene . ^^^
Interacting proteins: P15538 and P19099	Pubmed	SVM Score :0.0
The adrenocortical tumor cell line NCI H295R as an in vitro screening system for the evaluation of CYP11B2 ( aldosterone synthase ) and CYP11B1 ( steroid 11beta hydroxylase ) inhibitors . ^^^ To evaluate this cell line as a test system for effects and side effects of CYP inhibitors , we established assays using radiolabeled substrates of CYP11B2 and CYP11B1 and subsequently tested a series of CYP11B2 inhibitors including the CYP 19 inhibitor fadrozole . ^^^ Fadrozole and compounds 6 , 9 and 10 were more potent towards CYP11B2 compared to CYP11B1 with IC ( 50 ) values in the nanomolar range . ^^^
Interacting proteins: P15538 and P19099	Pubmed	SVM Score :0.0
Real time RT PCR showed that APAs produced higher levels of HSD3B2 , CYP 21 ( 21 hydroxylase ) , and CYP11B2 mRNA , whereas CPAs produced higher levels of CYP11A ( cholesterol side chain cleavage ) , CYP 17 ( 17alpha hydroxylase / 17 20 lyase ) , HSD3B2 , and CYP11B1 ( 11beta hydroxylase ) mRNA compared with normal adrenal . ^^^
Interacting proteins: P15538 and P19099	Pubmed	SVM Score :0.0
On the other allele we found a chimeric gene that consists of part of the aldosterone synthase gene ( CYP11B2 ) at exons 1 3 and part of the 11beta hydroxylase gene ( CYP11B1 ) at exons 4 9 . ^^^ G314R , in one CYP11B1 allele , and a chimeric CYP11B2 / CYP11B1 in the other allele . ^^^ The chimeric CYP11B2 / CYP11B1 protein retained 11beta hydroxylase enzymatic activity in vitro . ^^^ CONCLUSION : This case is caused by compound heterozygosity for a nonfunctional missense mutation and a chimeric CYP11B2 / CYP11B1 gene with hydroxylase activity that is controlled by the CYP11B2 promoter . ^^^
Interacting proteins: P15538 and P19099	Pubmed	SVM Score :0.0
In contrast , high concentrations of PCB 126 stimulated basal cortisol and aldosterone biosynthesis , including induction of CYP21B , CYP11B1 , and CYP11B2 mRNA expression and elevation of the conversion of cortisol from 17 OH progesterone and aldosterone from progesterone . cAMP abolished the positive effect of PCB 126 on cortisol synthesis , while it synergistically enhanced PCB 126 stimulation on CYP11B2 expression and aldosterone production . ^^^
Interacting proteins: P15538 and P19099	Pubmed	SVM Score :0.0
The activity of the compounds was determined using human CYP11B2 , and the selectivity was evaluated toward the human steroidogenic enzymes CYP11B1 , CYP 19 , and CYP 17 . ^^^ The biological results revealed a few rather selective inhibitors of CYP11B1 , some compounds inhibiting both CYP11B1 and CYP11B2 , and a large number of highly selective inhibitors of CYP11B2 . ^^^ The pyrimidyl substituted derivative 28a was found to be the most selective CYP11B2 inhibitor ( IC ( 50 ) = 27 nM ) in this series , showing a 120 fold selectivity for CYP11B1 ( IC ( 50 ) = 3179 nM ) . ^^^
Interacting proteins: P15538 and P19099	Pubmed	SVM Score :0.0
DESIGN : We measured mRNA levels of CYP11B2 [ presented as a ratio against glyceraldehyde 3 phosphate dehydrogenase ( B2 / G ) ] and CYP11B1 in relation to the K173R polymorphism . ^^^
Interacting proteins: P15538 and P19099	Pubmed	SVM Score :0.0
The aldosterone synthase ( CYP11B2 ) and 11beta hydroxylase ( CYP11B1 ) genes are not expressed in the rat heart . ^^^ Aldosterone synthase ( CYP11B2 ) and 11beta hydroxylase ( CYP11B1 ) catalyze the production of aldosterone and corticosterone , respectively , in the rat adrenal cortex . ^^^ Aldosterone synthase ( CYP11B2 ) and 11beta hydroxylase ( CYP11B1 ) catalyze the production of aldosterone and corticosterone , respectively , in the rat adrenal cortex . ^^^ To investigate this , we have used our established , highly sensitive real time quantitative RT PCR method to measure CYP11B1 and CYP11B2 mRNA levels in adrenal and cardiac tissue from several rat models of cardiovascular pathology . ^^^ There were no other significant differences in adrenal CYP11B2 or CYP11B1 expression between the model animals and their respective controls . ^^^
Interacting proteins: P15538 and P19099	Pubmed	SVM Score :0.0
After the chemical treatments , changes in mRNA expression of 11 steroidogenic genes ( CYP11A , CYP11B1 , CYP11B2 , CYP 17 , CYP 19 , CYP 21 , 3beta HSD 1 , 3beta HSD 2 , 17beta HSD 1 , StAR and HMGR ) were quantified using molecular beacon based real time RT PCR . ^^^ Genes coding for enzymes involved in the later or final steps of steroid production ( CYP11B1 , CYP11B2 , CYP 19 , 3beta HSD 1 , 3beta HSD 2 and 17beta HSD 1 ) were up regulated to various extents by most PCBs . ^^^ The greatest transcriptional activations ( 2 . 8 29 . 9 fold ) were elicited by PCB 110 on CYP11B1 , CYP11B2 , 3beta HSD 2 and CYP 19 , and PCB 149 on CYP11B1 , 3beta HSD 1 and 17beta HSD 1 . ^^^
Interacting proteins: P15538 and P19099	Pubmed	SVM Score :0.0
Activity of the compounds was determined in vitro using human CYP11B2 and selectivity was evaluated toward the human steroidogenic enzymes CYP11B1 , CYP 19 , and CYP 17 . ^^^ The 6 ethoxy derivative 5 was found to be the most selective CYP11B2 inhibitor ( IC 50 = 12 nM ; K ( 1 ) value = 8 nM ; CYP11B1 IC 50 = 5419 nM ; selectivity factor = 451 ) , showing no inhibition of human CYP3A4 ( 50 nM ) and CYP2D6 ( 20 nM ) . ^^^
Interacting proteins: P15538 and P19099	Pubmed	SVM Score :0.0
Molecular identity and gene expression of aldosterone synthase cytochrome P 450 . 11Beta hydroxylase ( CYP11B1 ) of bovine adrenal cortex produced corticosterone as well as aldosterone from 11 deoxycorticosterone in the presence of the mitochondrial P 450 electron transport system . ^^^ The expression of CYP11B1 gene was regulated by cyclic AMP ( cAMP ) dependent signaling , whereas that of CYP11B2 gene by calcium ion signaling as well as cAMP signaling . ^^^
Interacting proteins: P15538 and P19099	Pubmed	SVM Score :0.0
Glucocorticoid remediable aldosteronism ( GRA ) , also known as familial hyperaldosteronism type 1 ( FH 1 , OMIM 103900 ) , is a monogenic form of inherited hypertension caused by the presence of a chimaeric gene originating from an unequal cross over between the CYP11B1 ( 11beta hydroxylase ) and CYP11B2 ( aldosterone synthase ) genes . ^^^ The hybrid gene has the CYP11B1 sequence at the 5 ' end , including the promoter , and the CYP11B2 sequence at the 3 ' end . ^^^ None of our PHA or EH patients was positive for the CYP11B1 / CYP11B2 chimaeric gene . ^^^
Interacting proteins: P15538 and P19099	Pubmed	SVM Score :0.0
We report the successful application of a rapid , polymerase chain reaction ( PCR ) based method of detecting the ' hybrid ' 11 beta hydroxylase ( 11 beta OHase ) / aldosterone synthase ( AS ) gene as a screening test for FH 1 . ^^^
Interacting proteins: P15538 and P19099	Pubmed	SVM Score :0.0
Mechanistic study of polychlorinated biphenyl 126 induced CYP11B1 and CYP11B2 up regulation . ^^^ Our previous studies showed that high concentrations of PCB 126 stimulated CYP11B1 and CYP11B2 mRNA expression and consequently raised cortisol and aldosterone synthesis in the human adrenocortical H295R cells , respectively . ^^^ Although 3 ' , 4 ' DMF abolished AhR dependent transcriptional activity , it could not block PCB 126 stimulated CYP11B1 and CYP11B2 induction . ^^^ Furthermore , PCB 39 , 77 , 132 , 156 , and 169 , whether AhR ligands or not , all could increase CYP11B1 and CYP11B2 mRNA accumulation . ^^^ The synergistic induction of CYP11B1 and CYP11B2 mRNA levels by the PCB126 / 3 ' , 4 ' DMF cotreatment might result from the combination of transcriptional regulation by 3 ' , 4 ' DMF and posttranscriptional regulation by PCB 126 . ^^^
Interacting proteins: P15538 and P19099	Pubmed	SVM Score :0.0
During the past years , the necessary test systems have been developed and new compounds have been synthesized , which displayed selective and specific inhibition of CYP 17 , CYP11B2 , and CYP11B1 . ^^^
Interacting proteins: P15538 and P19099	Pubmed	SVM Score :0.0
The compounds were tested for activity by use of human CYP11B2 expressed in fission yeast and V 79 MZh cells and for selectivity by use of human CYP11B1 , CYP 17 , and CYP 19 . ^^^ The 5 methoxyindene 3 was found to be the most selective CYP11B2 inhibitor ( IC ( 50 ) = 4 nM ; CYP11B1 IC ( 50 ) = 5684 nM ) , which also showed only marginal inhibition of human CYP3A4 and CYP2D6 . ^^^
Interacting proteins: P15538 and P19099	Pubmed	SVM Score :0.0
In order to clarify the pathophysiological roles of adrenal RAS in aldosterone producing adenoma ( APA ) , we studied the expressions of the messenger RNAs ( mRNAs ) of renin , angiotensinogen , type 1 ( AT1R ) and type 2 angiotensin 2 receptor ( AT2R ) , CYP11B1 ( 11 beta hydroxylase gene ) and CYP11B2 ( aldosterone synthase gene ) in 8 patients with angiotensin 2 responsive ( ATII R ) APA and compared them with the expressions of the same mRNAs in 8 patients with angiotensin 2 unresponsive ( ATII U ) APA . ^^^ There were no significant differences between ATII R APA and ATII U APA in the mRNA levels of renin , angiotensinogen , AT 1 R , CYP11B1 and CYP11B2 . ^^^
Interacting proteins: P15538 and P19099	Pubmed	SVM Score :0.0
The expression of 21 hydroxylase ( CYP21A2 ) mRNA increased within 3 h of hCG administration , while 11beta hydroxylase 1 ( CYP11B1 ) and 11beta hydroxylase 2 ( CYP11B2 ) mRNAs were not detectable . 11beta Hydroxysteroid dehydrogenase 1 ( HSD11B1 ) mRNA had increased at 12 h after hCG administration , and 11beta hydroxysteroid dehydrogenase 2 ( HSD11B2 ) had decreased by 3 h after hCG administration . ^^^
Interacting proteins: P15538 and P19099	Pubmed	SVM Score :0.0
In Familial Hyperaldosteronism Type 1 ( FH 1 , glucocorticoid suppressible hyperaldosteronism ) , a curable form of hypertension inherited in an autosomal dominant fashion , the underlying genetic defect is a `` hybrid gene ' ' in which 11 beta hydroxylase gene regulatory elements are fused to the coding region of the aldosterone synthase gene . ^^^
Interacting proteins: P15538 and P19099	Pubmed	SVM Score :0.0
All patients had negative genetic testing for the defect associated with FH 1 , the CYP11B1 / CYP11B2 hybrid gene . ^^^
Interacting proteins: P15538 and P19099	Pubmed	SVM Score :0.0
Familial hyperaldosteronism type 1 ( FH 1 ) In FH 1 , inheritance of a ' hybrid ' 11beta hydroxylase / aldosterone synthase gene causes adrenocorticotrophic hormone ( ACTH ) regulated aldosterone and ' hybrid steroid ' ( 18hydroxy cortisol and 18 oxo cortisol ) overproduction . ^^^ Detailed biochemical studies , including analyses of aldosterone / PRA / cortisol ' day curve ' levels , have led to a fuller understanding of aldosterone regulation both before and in response to glucocorticoid treatment in this condition , and prompted a re examination of current approaches to treatment Unless ACTH is completely suppressed by glucocorticoid treatment , the hybrid gene dominates over the wild type aldosterone synthase genes in terms of aldosterone production , both in untreated and treated FH 1 . ^^^
Interacting proteins: P15538 and P19099	Pubmed	SVM Score :0.0
In familial hyperaldosteronism type 1 ( FH 1 ) , glucocorticoid remediable PAL is caused by inheritance of an ACTH regulated , hybrid CYP11B1 / CYP11B2 gene . ^^^ Analyses of aldosterone / PRA / cortisol ' day curves ' have revealed that ( 1 ) the hybrid gene dominates over wild type CYP11B2 in terms of aldosterone regulation and ( 2 ) correction of hypertension in FH 1 requires only partial suppression of ACTH , and much smaller glucocorticoid doses than those previously recommended . ^^^
Interacting proteins: P15538 and P19099	Pubmed	SVM Score :0.0
Familial hyperaldosteronism type 1 ( FH 1 ) is a glucocorticoid remediable form of PAL caused by the inheritance of an adrenocorticotrophic hormone ( ACTH ) regulated , hybrid CYP11B1 / CYP11B2 gene . ^^^
Interacting proteins: P15538 and P19099	Pubmed	SVM Score :0.0
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Interacting proteins: P15538 and P19099	Pubmed	SVM Score :0.0
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Interacting proteins: P15538 and P19099	Pubmed	SVM Score :0.0
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Interacting proteins: P15538 and P19099	Pubmed	SVM Score :0.0
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Interacting proteins: P15538 and P19099	Pubmed	SVM Score :0.0
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Interacting proteins: P15538 and P19099	Pubmed	SVM Score :0.0
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Interacting proteins: P15538 and P19099	Pubmed	SVM Score :0.0
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Interacting proteins: P15538 and P19099	Pubmed	SVM Score :0.0
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Interacting proteins: P15538 and P19099	Pubmed	SVM Score :0.0
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Interacting proteins: P15538 and P19099	Pubmed	SVM Score :0.0
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Interacting proteins: P15538 and P19099	Pubmed	SVM Score :0.0
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Interacting proteins: P15538 and P19099	Pubmed	SVM Score :0.0
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Interacting proteins: P15538 and P19099	Pubmed	SVM Score :0.0
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Interacting proteins: P15538 and P19099	Pubmed	SVM Score :0.0
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Interacting proteins: P15538 and P19099	Pubmed	SVM Score :0.0
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Interacting proteins: P15538 and P19099	Pubmed	SVM Score :0.0
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Interacting proteins: P15538 and P19099	Pubmed	SVM Score :0.0
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Interacting proteins: P15538 and P19099	Pubmed	SVM Score :0.0
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Interacting proteins: P15538 and P19099	Pubmed	SVM Score :0.0
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Interacting proteins: P15538 and P19099	Pubmed	SVM Score :0.0
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Interacting proteins: P15538 and P19099	Pubmed	SVM Score :0.0
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Interacting proteins: P15538 and P19099	Pubmed	SVM Score :0.0
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Interacting proteins: P15538 and P19099	Pubmed	SVM Score :0.0
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Interacting proteins: P15538 and P19099	Pubmed	SVM Score :0.0
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Interacting proteins: P15538 and P19099	Pubmed	SVM Score :0.0
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Interacting proteins: P15538 and P19099	Pubmed	SVM Score :0.0
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Interacting proteins: P15538 and P19099	Pubmed	SVM Score :0.0
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Interacting proteins: P15538 and P19099	Pubmed	SVM Score :0.0
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Interacting proteins: P15538 and P19099	Pubmed	SVM Score :0.0
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Interacting proteins: P15538 and P19099	Pubmed	SVM Score :0.0
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Interacting proteins: P15538 and P19099	Pubmed	SVM Score :0.0
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Interacting proteins: P15538 and P19099	Pubmed	SVM Score :0.0
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Interacting proteins: P15538 and P19099	Pubmed	SVM Score :0.0
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