| Interacting proteins: P55001 and P15502 |
Pubmed |
SVM Score :1.1052469 |
| To study possible molecular interactions between MAGP and other elastic fiber components , we have generated native MAGP using a baculovirus expression system and tested its ability to associate with tropoelastin and fibrillin . 1.1052469^^^ |
|
| Interacting proteins: P55001 and P15502 |
Pubmed |
SVM Score :0.60335532 |
| The interactions of MAGP 1 with type 6 collagen and tropoelastin were both determined to be of moderately high affinity , with Kd values of 5 . 6 10 10 ( 7 ) M and 2 . 6 10 10 ( 7 ) M , respectively . 0.60335532^^^ |
|
| Interacting proteins: P55001 and P15502 |
Pubmed |
SVM Score :0.57027788 |
| In addition , no evidence of an interaction was observed between MAGP 1 and a tropoelastin construct consisting of domains 17 27 that brackets the kallikrein cleavage site , suggesting a complex mechanism of interaction between the two molecules . 0.57027788^^^ To investigate the interaction of MAGP 1 with tropoelastin and fibrillin 1 , we expressed human MAGP 1 as a T 7 tag fusion protein in Escherichia coli . 0.50440789^^^ |
|
| Interacting proteins: P55001 and P15502 |
Pubmed |
SVM Score :0.0 |
| The elastic fiber consists of several components : a central core of alpha elastin and a microfibrillar sheath containing three components : fibrillin , microfibril associated glycoprotein , and a 35 kD protein with amine oxidase activity . ^^^ |
|
| Interacting proteins: P55001 and P15502 |
Pubmed |
SVM Score :0.0 |
| Fibrillin 1 , purified from tissue using reductive saline extraction , showed no binding to microfibril associated glycoprotein ( MAGP ) or tropoelastin . ^^^ |
|
| Interacting proteins: P55001 and P15502 |
Pubmed |
SVM Score :0.0 |
| Functional domains on elastin and microfibril associated glycoprotein involved in elastic fibre assembly . ^^^ Chondrocytes cultured in the presence of microfibril associated glycoprotein ( MAGP ) : 21 35 , an antibody directed against a domain near the N terminus of MAGP , did not organize tropoelastin into fibres . ^^^ |
|
| Interacting proteins: P55001 and P15502 |
Pubmed |
SVM Score :0.0 |
| Expression of fibrillin 1 , microfibril associated glycoprotein ( MAGP ) and elastin during the fetal period were compared with that of postnatal two year old animals ( heifers ) and adults . ^^^ |
|
| Interacting proteins: P55001 and P15502 |
Pubmed |
SVM Score :0.0 |
| There were no significant differences of steady state levels of elastin , fibrillin 1 and microfibril associated glycoprotein 1 mRNAs between patient and matched control fibroblasts . ^^^ |
|
| Interacting proteins: P55001 and P15502 |
Pubmed |
SVM Score :0.0 |
| Molecular interactions of biglycan and decorin with elastic fiber components : biglycan forms a ternary complex with tropoelastin and microfibril associated glycoprotein 1 . ^^^ |
|
| Interacting proteins: P55001 and P15502 |
Pubmed |
SVM Score :0.0 |
| Microfibril associated glycoprotein 36 ( MAGP 36 ) is a recently isolated elastin binding protein and considered to be a member of microfibril associated glycoproteins ( MAGPs ) . ^^^ |
|
| Interacting proteins: P55001 and P15502 |
Pubmed |
SVM Score :0.0 |
| METHODS : Postembedding immunoelectron microscopic studies were performed with antibodies specific for major ECM components , including fibronectin , laminin , vitronectin , tenascin , elastin , fibrillin 1 , microfibril associated glycoprotein ( MAGP ) 1 , decorin , versican , and five types of collagen ( 1 , 3 , 4 , 5 , and 6 ) . ^^^ |
|
| Interacting proteins: P55001 and P15502 |
Pubmed |
SVM Score :0.0 |
| Immunohistochemical analysis showed elastin , and microfibril associated proteins ( fibrillin 1 and 2 and microfibril associated glycoprotein 1 and 4 ) were absent or decreased in the papillary dermis and present in the accumulated elastic fibers in both lesions . ^^^ |
|
| Interacting proteins: P55001 and P15502 |
Pubmed |
SVM Score :0.0 |
| METHODS : Postembedding immunoelectron microscopic studies were performed with antibodies against myocilin and other ECM components , including fibronectin , laminin , vitronectin , tenascin , elastin , fibrillin 1 , microfibril associated glycoprotein ( MAGP ) 1 , decorin , versican , hyaluronic acid , and five types of collagen ( 1 , 3 , 4 , 5 , and 6 ) . ^^^ |
|
| Interacting proteins: P55001 and P15502 |
Pubmed |
SVM Score :0.0 |
| Microfibril Associated Glycoprotein 1 ( MAGP 1 ) binds fibrillin to tropoelastin during elastogenesis . ^^^ |
|
| Interacting proteins: P55001 and P15502 |
Pubmed |
SVM Score :0.0 |
| Da microfibril associated glycoprotein ( MAGP 36 ) is an elastin binding protein increased in chick aortae during development and growth . ^^^ |
|
| Interacting proteins: P55001 and P15502 |
Pubmed |
SVM Score :0.0 |
| Elastic tissue is composed of amorphous appearing elastin and 12 nm diameter microfibrils , one component of which has recently been isolated and characterized as the 31 KD microfibril associated glycoprotein MAGP . ^^^ Conditions have been defined that permit precise localization within the extracellular matrix of antibodies to MAGP and to elastin , singly and together . ^^^ |
|
| Interacting proteins: P55001 and P15502 |
Pubmed |
SVM Score :0.0 |
| We describe the generation of a monoclonal antibody library to ocular zonule components and the characterization of three monoclonal antibodies : 1 ) one specific for microfibrillar associated glycoprotein ( MAGP ) , a component of both ocular zonules and microfibrils of elastin fibers , 2 ) an antibody to an as yet unidentified 70 , 000 dalton antigen that is present in abundance in the extracellular matrix ( ECM ) of elastin producing cells , and 3 ) an antibody reacting with the 67000 dalton subunit of the elastin receptor . ^^^ |
|
| Interacting proteins: P55001 and P15502 |
Pubmed |
SVM Score :0.0 |
| In the present paper we compare the localisation of anti MAGP antibodies and anti tropoelastin antibodies in a range of bovine elastic and non elastic tissues . ^^^ |
|
| Interacting proteins: P55001 and P15502 |
Pubmed |
SVM Score :0.0 |
| In contrast , the adjacent ' elastin associated microfibrils ' showed a very strong immunoreaction with anti MP 340 and a moderate reaction with anti MAGP . ^^^ |
|
| Interacting proteins: P55001 and P15502 |
Pubmed |
SVM Score :0.0 |
| Elastin synthesis goes through several steps : gene transcription , alternative splicing of pre mRNA , mRNA translation , hydroxylation of some proline residues of the newly synthesized protein tropoelastin , association of with a 67 kDa chaperone protein , secretion of tropoelastin molecules in the extracellular space , and their deposition on the microfibrillar scaffold which contains fibrillin 1 , fibrillin 2 , MAGP 1 and MAGP 2 , . . . . ^^^ Elastin synthesis goes through several steps : gene transcription , alternative splicing of pre mRNA , mRNA translation , hydroxylation of some proline residues of the newly synthesized protein tropoelastin , association of with a 67 kDa chaperone protein , secretion of tropoelastin molecules in the extracellular space , and their deposition on the microfibrillar scaffold which contains fibrillin 1 , fibrillin 2 , MAGP 1 and MAGP 2 , . . . . ^^^ |
|
| Interacting proteins: P55001 and P15502 |
Pubmed |
SVM Score :0.0 |
| To address these possibilities , the expression of fibrillin 1 and fibrillin 2 was compared with expression of MAGP and tropoelastin in two elastogenic tissues that undergo different developmental programs . ^^^ |
|
| Interacting proteins: P55001 and P15502 |
Pubmed |
SVM Score :0.0 |
| Elastotic materials exhibited a strong reaction to the antibodies for elastin , microfibril associated glycoprotein 1 ( MAGP 1 ) , MAGP 4 , matrix metalloproteinase 1 ( MMP 1 ) , MMP 2 and MMP 3 , but a diminished reaction to anti MMP 9 antibody . ^^^ |
|
| Interacting proteins: P55001 and P15502 |
Pubmed |
SVM Score :0.0 |
| Microfibril associated glycoprotein 1 ( MAGP 1 ) and fibrillin 2 , the major components of microfibrils , provide the likely template for tropoelastin deposition . ^^^ An immunofluorescence study also confirmed that RNAi mediated down regulation of MAGP 1 or fibrillin 2 led to the loss of tropoelastin immunoreactivity . ^^^ These results suggest that MAGP 1 and fibrillin 2 are , directly or indirectly , associated with the extracellular deposition of tropoelastin during elastic fiber formation in human gingival fibroblasts in vitro . . ^^^ |
|
| Interacting proteins: P55001 and P15502 |
Pubmed |
SVM Score :0.0 |
| Heparin did not inhibit fibrillin 1 N and C terminal interactions or RGD dependent cell attachment , but heparin and MAGP 1 competed for binding to the fibrillin 1 N terminus , and heparin and tropoelastin competed for binding to a central fibrillin 1 sequence . ^^^ |
|
| Interacting proteins: P55001 and P15502 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P55001 and P15502 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P55001 and P15502 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P55001 and P15502 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P55001 and P15502 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P55001 and P15502 |
Pubmed |
SVM Score :0.0 |
| NA |
|