| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| Lysyl oxidase ( EC 1 . 4 . 3 . 13 ) is a copper dependent enzyme acting principally on collagen and elastin catalyzing the formation of aldehyde cross links . ^^^ |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| Inhibition of lysyl oxidase ( protein lysine 6 oxidase , EC 1 . 4 . 3 . 13 ) decreases the rate of collagen and elastin cross link formation and produces osteolathyrism in animals . ^^^ |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| Lysyl oxidase ( EC 1 . 4 . 3 . 13 ) initiates the crosslinking of collagens and elastin by catalyzing oxidative deamination of the epsilon amino group in certain lysine and hydroxylysine residues . ^^^ |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| Isonicotinic acid hydrazide ( isoniazid ) causes a large increase in the salt solubility of collagen when injected into chick embryos ; this change is accompanied by the inactivation of lysyl oxidase ( EC 1 . 4 . 3 . 13 ) , the enzyme responsible for initiating cross link formation in collagen and elastin . ^^^ |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| Lysyl oxidase ( EC 1 . 4 . 3 . 13 ) , an extracellular copper amino oxidase , initiates the cross linking of collagens and elastin by catalyzing oxidative deamination of the epsilon amino group in certain lysine and hydroxylysine residues . ^^^ |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| Lysyl oxidase ( EC 1 . 4 . 3 . 13 ) , an extracellular copper enzyme , initiates the crosslinking of collagens and elastin by catalyzing oxidative deamination of the epsilon amino group in certain lysine and hydroxylysine residues . ^^^ |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| Lysyl oxidase ( EC 1 . 4 . 3 . 13 ) , a copper dependent enzyme which catalyses the formation of aldehyde cross links , and acts primarily on collagen and elastin , is known to be increased during wound healing and in fibrotic disorders including liver cirrhosis and atherosclerosis , and to be decreased in some hereditary connective tissue diseases and in malignant cell lines . ^^^ |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| These abnormalities are associated with a reduction in the activity of lysyl oxidase ( EC 1 . 4 . 3 . 13 ) , an extracellular copper enzyme that initiates the crosslinking of collagens and elastin . ^^^ |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| Lysyl oxidase ( EC 1 . 4 . 3 . 13 ) oxidizes peptidyl lysine to peptidyl aldehyde residues within collagen and elastin , thus initiating formation of the covalent cross linkages that insolubilize these extracellular proteins . ^^^ |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| Lung lysyl oxidase and elastin synthesis during compensatory lung growth . ^^^ |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| The disappearance of the radioactivity from the soluble fraction and its appearance in the desmosines of elastin were inhibited by beta aminopropionitrile , a specific inhibitor of the cross linking enzyme lysyl oxidase . ^^^ This precursor protein is soluble elastin , as demonstrated by the formation of desmosines when a homogeneous preparation of soluble elastin was incubated with purified lysyl oxidase . . ^^^ |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| Lysyl oxidase the enzyme which oxidately deaminates lysine residues in collagen and elastin , was purified from embryonic chick cartialge by employing an affinity column of lathyritic rat skin collagen coupled to Sepharose , followed by separation on DEAE cellulose . ^^^ |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| The enzyme lysyl oxidase which catalyses the aldehyde formation in certain lysyl residues of collagen and elastin was extracted from the skin of hypophysectomized rats . ^^^ |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| Lysyl oxidase is a specific amine oxidase that catalyzes the formation of aldehyde cross link intermediates in collagen and elastin . ^^^ |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| Studies on the action of lysyl oxidase on soluble elastin . ^^^ |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| The activity of lysyl oxidase which catalyzes the initial step of cross linking of collagen and elastin polypeptides was measured in blood vessels of the hypertensive rat . ^^^ |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| Lysyl oxidase catalyzes the crosslinking of collagen and elastin . ^^^ |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| Studies on factors influencing the formation of desmosines by lysyl oxidase action on tropoelastin . ^^^ The formation of isodesmosine and desmosine in vitro by the action of lysyl oxidase on tropoelastin was studied . ^^^ |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| The activity of lysyl oxidase ( LOX ) , the extracellular enzyme responsible for initiating crosslinking of collagen and elastin , was measured during 3 types of postnatal lung growth . ^^^ |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| Lysyl oxidase is the copper dependent enzyme responsible for the normal cross linking of both collagen and elastin which is necessary for their functional integrity . ^^^ It is suggested that this lesion is the result of imperfect cross linking of arterial elastin as well as collagen , and is caused by a maternal deficiency of vitamin B 6 which is commonly found in pregnancy and which could thus impair the function of lysyl oxidase . ^^^ |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| Enzymatic , chromatographic and immunochemical tests disclosed that pure bovine aorta amine oxidase was not a lysyl oxidase capable of oxidizing the lysine residues of tropoelastin to allysine ; The bovine aorta amine oxidase preparation used by Rucker and O ' Dell appears to have been contaminated with lysyl oxidase which is the emzyme that oxidizes some of the lysine residues in tropoelastin and tropocollagen to allysine . . ^^^ |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| Nutritional copper deficiency effects marked changes in the crosslinking of collagen and elastin , presumably in relationship to copper ' s role as a cofactor for lysyl oxidase . ^^^ Lysyl oxidase controls one of the initial steps in the crosslinking of elastin and collagen , i . e . , the conversion of peptidyl lysine or hydroxylysine residues to peptidyl alpha aminoadipic delta semialdehyde derivatives . ^^^ |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| Various o and p quinones were assessed as oxidants of peptidyl lysine in elastin and collagen substrates in the presence and absence of divalent copper as paradigms of protein lysine 6 oxidase ( lysyl oxidase ) which contains both quinone and copper cofactors . ^^^ |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| Lysyl oxidase , which cross links collagen and elastin , was obtained from chick embryo bone and cartilage and its substrate , elastin , from aorta . ^^^ |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| The developmental steady state levels of lysyl oxidase mRNA together with the mRNAs encoding two of the enzyme ' s substrates ( tropoelastin and type 1 collagen ) increased between 8 and 16 days of embryonic development . ^^^ In situ hybridization showed that the spatial expressions of lysyl oxidase and tropoelastin transcripts differ suggesting that the enzyme and substrate genes are differentially regulated within the cells of the arterial wall . . ^^^ |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| A 258 bp segment of the 3 ' untranslated region of lysyl oxidase cDNA is 93 % identical with a highly conserved region of the 3 ' untranslated sequence of rat elastin cDNA . ( ABSTRACT TRUNCATED AT 250 WORDS ) . ^^^ |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| These studies indicate that the inhibition of lysyl oxidase by BAPN prevents elastin crosslinking which results in an increase in tropoelastin moieties , thus leading to a down regulation of the steady state levels of elastin mRNA . . ^^^ |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| The synthesis of lysyl oxidase , which initiates the cross linking of collagen and elastin , was investigated in carbon tetrachloride ( CCl 4 ) induced fibrotic liver of rat . ^^^ |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| Lysyl oxidase catalyzes the oxidation of peptidyl lysine to alpha aminoadipic delta semialdehyde , the precursor to the covalent crosslinkages that stabilize fibers of elastin and collagen . ^^^ |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| A modification of the original microdistillation assay for lysyl oxidase is described in which Amicon C 10 microconcentrators are used to separate , by ultrafiltration , the 3H labeled products released from a [ 4 , 5 3H ] lysine labeled elastin substrate . ^^^ |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| Lysyl oxidase initiates the cross linking of collagen and elastin by catalyzing the formation of the lysine derived aldehyde . ^^^ |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| We have measured the activity of lysyl oxidase , the copper dependent enzyme that initiates the cross linking of collagen and elastin , in extracts of skin and aorta obtained at autopsy from a patient with unusually marked connective tissue manifestations , and found it to be only 6 12 % of normal , thus suggesting a basis for these alterations . . ^^^ |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| Treatment with beta aminopropionitrile ( BAPN , an inhibitor of lysyl oxidase , the enzyme involved in the formation of cross links in elastin and collagen ) increased the formation of IEL ruptures in both strains in the caudal and renal artery and in the abdominal aorta in BN rats , but not in the abdominal aorta of LE rats . ^^^ |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| Contrary to what was observed by Kagan and coworkers on bovine aorta by using antibodies against aortic lysyl oxidase ( Kagan et al . , 1986 ) , no specific localization of gold particles could be observed on or adjacent to the elastin / associated microfibrils . ^^^ The results indicate that antibodies raised against placenta lysyl oxidase recognize collagen associated as well as elastin associated epitopes . ( ABSTRACT TRUNCATED AT 250 WORDS ) . ^^^ |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| The latter was chosen as a model of the connective tissue , and was analyzed by means of electron microscopy and by measurement of the activity of lysyl oxidase , the enzyme involved in collagen and elastin crosslink formation . ^^^ |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| Cultured vascular endothelium secretes the enzyme lysyl oxidase which cross links both collagen and elastin . ^^^ |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| In parallel with the increase in lysyl oxidase activity , the rate of 14C lysine incorporation into desmosine and isodesmosine was at its maximum 1 wk after elastase administration , reflecting the lysyl oxidase mediated cross link formation , which is the final step in the resynthesis of the pulmonary elastin destroyed by the elastolytic insult . . ^^^ |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| Formation of new elastin as monitored by 14C lysine incorporation into the elastin specific cross links desmosine and isodesmosine was reduced approximately 40 % , although there was no significant difference in the levels of lysyl oxidase activity . ^^^ |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| Lysyl oxidase , the enzyme responsible for mediating crosslink formation in collagen and elastin , requires copper for its activity . ^^^ In this study , lysyl oxidase activity and insoluble elastin content were unchanged in lungs from copper deficient hamsters compared to controls . ^^^ |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| Extracts of bovine ligamentum nuchae have been assayed for lysyl oxidase activity using as substrates soluble elastin and soluble collagen labeled with tritiated lysine . ^^^ |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| Lung lysyl oxidase activity was lower in Cu deficient rats , and data for the relative distribution of lung elastin cross linking amino acids indicated impaired cross linking in the pups from both the Cu deficient and the 0 . 4 % DPA groups . ^^^ |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| A deficiency of copper in the diet is known to inhibit lysyl oxidase , a metalloenzyme requiring copper as a cofactor and which is also responsible for collagen and elastin crosslinking . ^^^ |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| The activity of lysyl oxidase , the cross linking enzyme of elastin and collagen , was measured in culture media of human skin fibroblasts , human aortic medial smooth muscle cells ( SMCs ) and adventitial fibroblasts using [ 3H ] lysine labelled elastin substrate . ^^^ |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| Previous studies have pointed towards a cofactor role for pyridoxal 5 ' phosphate ( PLP ) in lysyl oxidase , the enzyme that generates the peptidyl aldehyde precursor to the lysine derived cross linkages in elastin and collagen . ^^^ |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| Lysyl oxidase is an extracellular enzyme that initiates crosslink formation in the major connective tissue proteins , the collagens and elastin . ^^^ |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| Lysyl oxidase was similarly localized in newborn rat aorta at the interface between microfibrils and nascent elastin fibers . ^^^ |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| To study the interactions of lipoproteins , connective tissue components and cells , mouse peritoneal macrophages were incubated in the presence of human low density lipoproteins ( LDL ) that had been complexed with pig aortic proteoglycans ( PG ) or incubated in the presence of soluble collagen and / or lysyl oxidase , which catalyses the formation of cross linkages in collagen and elastin by oxidising epsilon amino groups of lysine residues to aldehydes . ^^^ |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| Lysyl oxidase activity and elastin / glycosaminoglycan interactions in growing chick and rat aortas . ^^^ The number and size of the abnormal elastin / GAGs aggregates were proportional to the extent of lysyl oxidase inhibition . ^^^ All data suggest that , upon inhibition of lysyl oxidase , matrix GAGs remain among elastin molecules during fibrogenesis by binding to positively charged amino groups on elastin . ^^^ Data are presented suggesting that matrix glycosaminoglycans ( GAGs ) prevent spontaneous tropoelastin aggregation in vivo , at least up to the deamination of lysine residues on tropoelastin by matrix lysyl oxidase . ^^^ |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| Substituted benzylamines with increasingly electronegative para substituents had the lowest KI values and thus were the most effective inhibitors of the oxidation of elastin by lysyl oxidase . ^^^ |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| Since lysyl oxidase activity is influenced by ionic ligands bound to its protein substrates , the effect of heparin , an anionic glycosaminoglycan known to bind to collagen , was explored by using collagen and elastin substrates and highly purified lysyl oxidase . ^^^ These results suggest that heparin may modulate the oxidation and thus the insolubilization of extracellular collagen fibers , possibly under conditions where elastin fiber synthesis is not affected , and that the tight binding of lysyl oxidase to collagen is not completely related to the expression of enzyme activity toward this substrate . ^^^ |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| It is speculated that , at least in the system under study , the epsilon amino groups of tropoelastin molecules may offer the binding sites for matrix proteoglycans until they are removed by lysyl oxidase , and that matrix proteoglycans might play a role in elastin fibrogenesis by preventing spontaneous tropoelastin aggregation in areas far from growing elastin fibers . . ^^^ On the contrary , beta APN , a classical inhibitor of lysyl oxidase , induced the tropoelastin molecules to aggregate into abnormal protuberances on the old fibers . ^^^ |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| In the caudal and renal arteries of the male Wistar rat , interruptions in the internal elastic lamina ( IEL ) form spontaneously with age . beta aminopropionitrile ( BAPN ) , which is an inhibitor of the enzyme lysyl oxidase implicated in the synthesis of elastin , is able to induce in the young Wistar rat interruptions in the IEL which are morphologically very similar to those which form spontaneously . ^^^ |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| The paucity of elastin is attributed to a loss of lysyl oxidase activity . ^^^ |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| I . examined , a severe lysyl oxidase deficiency could account for the observed ultrastructural abnormalities of elastin and that , besides defects of collagen type 1 , additional alterations of cellular metabolism might be responsible for the clinical heterogeneity of the disease . . ^^^ |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| These findings suggest that impairment of the production of lysyl oxidase and the resynthesis of cross linked elastin by smoke inhalation exacerbates alveolar destruction . . ^^^ |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| The relative activity of connective tissue lysyl oxidase and plasma amine oxidase on collagen and elastin substrates . ^^^ |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| Desmosine and isodesmosine , cross linking compounds associated with mature elastin , were found in hydrolysates of the shell membrane , confirming the necessity for lysyl oxidase in its biosynthesis . ^^^ |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| Repeat polypeptide models of elastin as substrates for lysyl oxidase . ^^^ Synthetic repeat polypeptides analogous in sequence to the valine rich regions of elastin have been tested as substrates for purified bovine aorta lysyl oxidase . ^^^ These polypeptides , HCO ( phi Pro Gly Gly ) n Val OMe , HCO ( Val Pro Gly phi Gly ) n Val OMe , and HCO Val ( Ala Pro Gly phi Gly Val ) n OMe , where phi = Val or Lys at approximately a 4 : 1 ratio and where n greater than or equal to 40 , are models of the tetra , penta , or hexapeptide repeat sequences found in elastin . alpha Aminoadipic delta semialdehyde is generated in each of these upon incubation with lysyl oxidase at 37 degrees C , whereas the aldol and anhydrolysinonorleucine bifunctional cross linkages were formed only in the incubation of enzyme with polypentapeptide . ^^^ |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| The amounts of lysyl derived aldehydes ( the product formed in collagen and elastin by lysyl oxidase ) and of cross links formed from these products were decreased in dermal fibroblasts in culture . ^^^ |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| Lysyl oxidase activity against both collagen and elastin substrates has been examined in the culture medium of skin fibroblasts derived from unrelated patients with Menkes ' syndrome and from control subjects . ^^^ Lysyl oxidase activity in the culture medium of two of the Menkes ' fibroblast lines was also examined by using a crude aortic elastin substrate and was similarly decreased in comparison with that in the medium of control fibroblasts . ^^^ Lysyl oxidase activity in the medium of a fourth fibroblast line , derived from a foetus with Menkes ' syndrome , was 42 % of that in the medium of control fibroblasts derived from a 1 day old baby against a collagen substrate , and 26 % of that in control fibroblast medium against an elastin substrate . ^^^ |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| Substrate directed modulation of elastin oxidation by lysyl oxidase . ^^^ The oxidation of an elastin substrate by highly purified bovine aortic lysyl oxidase ( LO ) is markedly influenced by amphiphilic molecules known to bind to elastin . ^^^ |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| Lysyl oxidase : preparation and role in elastin biosynthesis . ^^^ |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| The copper requiring enzyme , lysyl oxidase , catalyzes the cross linking of tropoelastin into mature elastin . ^^^ |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| A postulated mechanism for these alterations in pulmonary connective tissue productive of malformations which model human tracheobronchomegaly and cystic lungs , is interference with lysyl oxidase , a copper requiring enzyme , which cross links elastin . . ^^^ |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| Reaction of aortic lysyl oxidase with beta aminopropionitrile . beta Aminopropionitrile ( BAPN ) is a potent irreversible inhibitor of lysyl oxidase , the enzyme which initiates cross linkage formation in elastin and collagen . ^^^ The initial interaction of BAPN with aortic lysyl oxidase is competitive with elastin or alkyl amine substrates . ^^^ |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| Effect of varying amounts of ascorbate on collagen , elastin and lysyl oxidase synthesis in aortic smooth muscle cell cultures . ^^^ In the presence of ascorbate , there is an increase in collagen synthesis with a concomitant decrease in insoluble elastin and lysyl oxidase activity in cultured rabbit aortic smooth muscle cells . ^^^ While the addition of 0 . 5 micrograms ascorbate per ml of medium enhances collagen synthesis and accumulation , detectable insoluble elastin and lysyl oxidase activity remain essentially unchanged . ^^^ However , at 2 micrograms ascorbate per ml , the integrity of the insoluble elastin is lost and lysyl oxidase activity is decreased . ^^^ |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| Lysyl oxidase initiates the covalent cross linking of elastin and collagen , converting lysyl residues in these proteins to peptidyl aldehyde residues . ^^^ |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| Lysyl oxidase initiates the covalent cross linking of elastin and collagen by oxidizing lysine residues in these proteins to alpha aminoadipic delta semialdehyde . ^^^ |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| After packaging into membrane bound vesicles in the Golgi apparatus , tropoelastin is secreted by exocytosis into the extracellular space where it is cross linked by a copper requiring extracellular enzyme , lysyl oxidase . ^^^ |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| Rheumatoid arthritis can be divided into two syndromes , one a potassium deficiency , the other an inappropriate copper requirement seriously affecting the elastin tissues through reduced lysyl oxidase cross linking . ^^^ |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| Propranolol acts , in part , by ( a ) stimulating lysyl oxidase to produce greater amounts of reactive aldehydes for intermolecular cross links , ( b ) enhancing the progression of chemically unstable to stable forms of intermolecular elastin cross links ( lysinonorleucine and the desmosines ) , and ( c ) reducing the density of the age related intermolecular cross linking of collagen ( pyridinoline ) . ^^^ |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| These same smoke components also suppress lysyl oxidase catalyzed oxidation of lysine epsilon amino groups in tropoelastin ( the chemical step preceding formation of all elastin cross links , including desmosine ) in a dose dependent fashion . ^^^ |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| The synthesis of collagen ( measured as the formation of non dialyzable hydroxyproline ) and other proteins , and the activity of lysyl oxidase , the enzyme responsible for the formation of cross links in elastin and collagen , were measured in cultured human aortic smooth muscle cells in the presence of various cortisol concentrations . ^^^ |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| Syncatalytic inactivation of lysyl oxidase might represent a means of restricting the activity of this enzyme toward its elastin and collagen substrates in vivo . . ^^^ |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| The spontaneously aneurysm prone Blotchy mouse has a mutation on the 10 chromosome resulting in low hepatic copper levels ; and copper is an essential cofactor for lysyl oxidase , which catalyzes reactions leading to the cross linking of collagen and elastin . ^^^ |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| The authors suggest that copper may be a factor in the development of scoliosis since it is part of the lysyl oxidase enzymes that are required for cross linking of collagen and elastin . ^^^ |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| In conclusion , deposition and maturation of elastin on microfibrils are necessary to form the microfibril bundles in normal elastogenesis , and vaguely outlined aggregated masses of elastin are formed under the inhibition of lysyl oxidase . . ^^^ |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| Although this domain contains the characteristic paired lysine residues found in other cross linking domains of elastin , protein sequence analysis indicated that the first but not the second lysine had been oxidized by lysyl oxidase . ^^^ |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| A novel human cDNA with a predicted protein homologous to the carboxyl end of lysyl oxidase , an extracellular enzyme involved in the maturation of collagen and elastin , has been isolated . ^^^ |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| We conclude that the elastin like sequence previously reported in rat lysyl oxidase cDNA is not a species specific sequence , and most probably resulted from spurious ligation reactions during construction of the cDNA library . . ^^^ |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| Lysyl oxidase oxidizes peptidyl lysine in collagen and elastin substrates to residues of alpha aminoadipic delta semialdehyde . ^^^ |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| The lysyl oxidase catalyzes the first step of collagen and elastin enzymatic cross linking and appears to be a crucial factor in stabilizing the neosynthesized extracellular matrix in the liver . ^^^ |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| A deficiency of the copper metalloenzyme , lysyl oxidase , results in a deficiency of lysyl derived cross linkages in collagen and elastin . ^^^ |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| These cross linking residues are formed by the action of lysyl oxidase upon Lys residues in tropoelastin , a precursor of elastin . ^^^ |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| Correlations between elastin and collagen type 1 mRNA expression and lysyl oxidase activity . ^^^ We also measured the activity of lysyl oxidase , an enzyme which plays a crucial role in the formation of crosslinks in both procollagen and tropoelastin molecules . ^^^ |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| Lysyl oxidase initiates crosslink formation of the collagen and elastin extracellular matrix , thereby delimiting its expansive properties . ^^^ These results support the ordered genesis of the collagen substrate > lysyl oxidase enzyme > elastin substrate . . ^^^ |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| Although cutis laxa acquisita is a heterogeneous group of disorders , findings in this patient were consistent with excessive loss of cutaneous elastin due to the combined effects of several factors , such as low lysyl oxidase activity together with high levels of cathepsin G and reduction of circulating proteinase inhibitor ( s ) . . ^^^ |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| CONCLUSIONS : The mechanism of the decrease in tropoelastin insolubilization was unrelated to lysyl oxidase activity in the tissue and represents a unique developmental program . . ^^^ |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| Expression and accumulation of lysyl oxidase , elastin , and type 1 procollagen in human Menkes and mottled mouse fibroblasts . ^^^ A decrease in the functional activity of lysyl oxidase , a cuproenzyme , is thought in part to be responsible for the decreased crosslinking of collagen and elastin . ^^^ Based on the present studies in cell culture , the production of essential enzymes and matrix proteins , such as lysyl oxidase and elastin , appear to be altered at the level of transcription or mRNA turnover . . ^^^ |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| The possibility was also assessed that an insoluble elastin like product could be generated from this elastin precursor in the absence of other macromolecules found associated with elastin in vivo . rTE was more efficiently oxidized by lysyl oxidase than the insoluble chick aorta elastin substrate conventionally used . ^^^ Incubation of rTE delta 26A with lysyl oxidase not only resulted in the generation of peptidyl alpha aminoadipic delta semialdehyde and lysine derived cross linkages , but also yielded a product insoluble in hot 0 . 1 N NaOH , consistent with the properties of insoluble elastin . ^^^ Thus , oxidation , cross linking and insolubilization of elastin substrates by lysyl oxidase can occur in the absence of other macromolecules implicated as being involved in this process in vivo , although such macromolecules may be essential to obtain the proper alignment between tropoelastin units for specifically placed cross linkages and optimally functional elastic fibers . . ^^^ Oxidation , cross linking , and insolubilization of recombinant tropoelastin by purified lysyl oxidase . ^^^ The use of recombinant human tropoelastin ( rTE ) and selected variants thereof as substrates for the assay of lysyl oxidase activity in vitro was explored . ^^^ |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| Reaction of lysyl oxidase with trans 2 phenylcyclopropylamine . trans 2 Phenylcyclopropylamine hydrochloride ( tranylcypromine ; TCP ) was found to be both an inhibitor and a substrate of lysyl oxidase , the enzyme which oxidizes peptidyl lysine in elastin and collagen to initiate cross linking in these proteins . ^^^ In contrast to the irreversible and / or competitive inhibitors of lysyl oxidase previously described , TCP noncompetitively and reversibly inhibited the oxidation of both alkylamine and elastin substrates with Ki values of 386 and 375 microM , respectively . ^^^ |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| Lysyl oxidase , an extracellular enzyme involved in the maturation of collagen and elastin , also appears to function as a phenotypic suppressor of transformation by the ras gene product , p 21 . ^^^ |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| Lysyl oxidase initiates cross linkage of collagen and elastin by catalysing the formation of a lysine derived aldehyde . ^^^ |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| Both cell lines secreted apparently identical , 29 kDa forms of mature lysyl oxidase each of which catalyzed the deamination of human recombinant tropoelastin and alkylamines , consistent with the known specificity of lysyl oxidase . ^^^ |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| Studies of the biochemistry of the aortic wall have shown that Propranolol has an independent effect on blood pressure , stimulation lysyl oxidase and production of intermolecular elastin bridges which strengthen the arterial wall . ^^^ |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| Lysyl oxidase catalyzes the final known enzymatic step required for collagen and elastin cross linking . ^^^ |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| Pharmacokinetic analysis of beta aminopropionitrile in rabbits . beta Aminopropionitrile ( beta APN ) , inhibits the activity of lysyl oxidase , an important enzyme for the post translational formation of inter and intramolecular covalent cross linking between the connective tissue proteins , collagen and elastin . ^^^ |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| PURPOSE : This study was designed to define the effects of beta adrenergic blockade on aortic lysyl oxidase ( LO ) , an enzyme responsible for elastin and collagen cross linking , and aneurysm formation in the blotchy mouse . ^^^ |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| When assayed using an elastin substrate , the activity of lysyl oxidase is unaffected by TRAMP . . ^^^ |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| The 72 kDa biosynthetic precursor , tropoelastin , is secreted into the extracellular space where it becomes highly cross linked into a rubber like network through the activity of the copper requiring enzyme lysyl oxidase . ^^^ |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| Lysyl oxidase activity was assayed with [ 4 , 5 3H ] lysine labelled purified chick embryo aorta elastin substrate . ^^^ |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| These results raise the possibility that extracellular lysyl oxidase may have important roles to play in biology in addition to its established function in the crosslinking of elastin and collagen . . ^^^ |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| Alterations in the synthesis and activity of lysyl oxidase occur concomitant with developmental changes in collagen and elastin deposition and with the pathogenesis of several acquired and heritable connective tissue disorders . ^^^ |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| Lysyl oxidase ( LO ) is a copper amine oxidase of the extracellular matrix which initiates covalent cross linking in collagens and elastin . ^^^ |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| Lysyl oxidase ( LO ) , an extracellular enzyme catalysing the first step of collagen and elastin cross linking , is transiently expressed by myofibroblasts during fibrosis . ^^^ |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| Lysyl oxidase is involved in the main pathway of collagen and elastin cross linking : it has a role in the maturation of fibrillar matrix proteins in fibrosing processes and dictates their stability against metalloproteases . ^^^ |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| Lysyl oxidase is an enzyme that catalyzes an essential step in collagen and elastin cross linking in the extracellular matrix , and TGF beta 1 has been implicated in the pathogenesis of restenosis after vascular injury . ^^^ |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| These are covalently cross linked to form insoluble elastin by formation of desmosine and isodesmosine , catalysed by the enzyme lysyl oxidase . ^^^ The amnion , chorion and decidua were shown by Northern analysis and RT PCR to contain detectable levels of tropoelastin mRNA and the mRNA encoding lysyl oxidase . ^^^ |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| Thus , we investigated the deposition of extracellular matrix components ( laminin , fibronectin EIIIA , collagen 1 and 4 , procollagen 3 , elastin , tenascin ) as well as the expression of lysyl oxidase and of alpha SM actin in the portal zone at 24 , 48 , and 72 hours and 7 days after ligation of the common bile duct . ^^^ |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| Lysyl oxidase , which catalyzes the corresponding aldehyde formation from certain lysyl and hydroxylysyl residues in collagen and elastin in the first step of the linking reaction , is the only known enzyme required in the crosslinking process . ^^^ |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| Lysyl oxidase is a copper requiring enzyme that catalyzes the oxidative deamination of lysyl residues linking two adjacent chains of tropoelastin polypeptides into an insoluble network . ^^^ |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| Lysyl oxidase is a copper dependent enzyme involved in extracellular processing of collagens and elastin . ^^^ |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| By comparing the PCR amplification products of both the cellular mRNA and a synthetic template , pMATRIX , it was possible to measure the direct expression of collagens alpha 2 ( 1 ) , alpha 1 ( 3 ) , alpha 1 ( 4 ) , fibronectin , laminin B 2 , elastin and lysyl oxidase genes . ^^^ |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| The lysyl oxidase like ( LOXL ) gene is a new member of the lysyl oxidase family , a copper dependent enzyme that is implicated in the crosslinking of collagen and elastin fibers . ^^^ |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| Lysyl oxidase ( LO ) initiates the first step in the crosslinking of collagens and elastin and has also been shown to function as a tumor suppressor . ^^^ |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| Lysyl oxidase ( LO ) is a copper dependent amine oxidase that plays a critical role in the biogenesis of connective tissue matrices by crosslinking the extracellular matrix proteins , collagen and elastin . ^^^ |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| These abnormalities are associated with a severe reduction in the activity of lysyl oxidase , the extracellular copper enzyme that initiates crosslinking of collagens and elastin . ^^^ |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| Steady state levels of tropoelastin and lysyl oxidase mRNA at 2 , 4 , and 7 days after elastase treatment were determined by Northern blot analysis . ^^^ Steady state levels of tropoelastin and lysyl oxidase mRNA showed no increase over control levels at 2 , 4 , and 7 days after elastase treatment . ^^^ |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| Protein lysine 6 oxidase ( lysyl oxidase ) is a cuproenzyme that is essential for stabilization of extracellular matrixes , specifically the enzymatic cross linking of collagen and elastin . ^^^ |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| Lysyl oxidase is the extracellular enzyme that catalyzes oxidative deamination of peptidyl lysine residues in elastin precursors , and lysine and hydroxylysine residues in collagen precursors to form peptidyl aldehydes . ^^^ |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| These cofactors are ( a ) topa quinone in copper containing amine oxidases , enzymes found in nearly all forms of life , including human ; ( b ) lysyl topa quinone of the copper protein lysyl oxidase , an enzyme required for proper cross linking of collagen and elastin ; ( c ) tryptophan tryptophylquinone of alkylamine dehydrogenases from gram negative soil bacteria ; and ( d ) the copper complexed cysteinyltyrosyl radical of fungal galactose oxidase . ^^^ |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| Following lysyl oxidase modification of tropoelastin lysine residues , they are released from glycosaminoglycan interactions , thereby permitting those residues to contribute to elastin cross links . . ^^^ |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| Localization of MAGP 36 in elastic fibers coincided with the distribution of lysyl oxidase , an enzyme that plays a pivotal role in the deposition of tropoelastin . ^^^ |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| Lysyl oxidase ( LO ) takes part in the initial steps of converting soluble monomers of collagen and elastin into insoluble fibres in the extracellular matrix . ^^^ |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| Lysyl oxidase ( LO ) is a key participant in the accumulation of insoluble fibers of elastin and collagen by virtue of its role in the initiation of the covalent cross linkages between and within individual molecules comprising these fibers . ^^^ |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| Lysyl oxidase catalyzes the final known enzymatic step required for cross linking collagen and elastin in the synthesis of a functional extracellular matrix . ^^^ This study investigated the regulation by TGF beta 1 of lysyl oxidase and its collagen and elastin substrates in early passage human gingival fibroblasts . ^^^ The results show that TGF beta 1 increases lysyl oxidase enzyme activity and mRNA levels for lysyl oxidase and alpha 1 type 1 collagen , but not elastin , in a dose and time dependent manner . ^^^ |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| The promoter activity of lysyl oxidase ( LOX ) , the enzyme involved in collagen and elastin cross linking and in tumor suppression , was compared in extracellular matrix producing cells and in tumorigenic c Ha ras NIH 3T3 fibroblasts ( RS 485 ) . ^^^ |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| Abnormal biochemistry in deficiency probably contributes to these lesions , e . g . , decreased activities of lysyl oxidase and superoxide dismutase which result in failure of collagen and elastin crosslinking and impaired defense against free radicals . ^^^ |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| Lysyl oxidase is an essential catalyst for the cross linking of extracellular collagen and elastin . ^^^ |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| Lysyl oxidase ( LO ) , an enzyme secreted by vascular smooth muscle cells ( VSMC ) , initiates the covalent crosslinking of polypeptide chains within collagen and elastin . ^^^ |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| Lysyl oxidase activity is critical for the assembly and cross linking of extracellular matrix proteins , such as collagen and elastin . ^^^ |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| Lysyl oxidase is an extracellular enzyme that controls the maturation of collagen and elastin . ^^^ |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| Aortic wall concentrations of desmosine ( Des ) and hydroxyproline ( OHP ) were measured at each interval , and the expression of tropoelastin ( TE ) , alpha 1 ( 1 ) procollagen ( PC ) , and lysyl oxidase genes was evaluated by reverse transcription polymerase chain reaction . ^^^ |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| Lysyl oxidase enzymatically crosslinks members of the extracellular matrix , including elastin and collagen . ^^^ |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| Lysyl oxidase ( LOX ) is the extracellular enzyme that initiates the main pathway of collagen and elastin cross linking . ^^^ |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| To clarify the roles of enzymes in collagen degradation of the follicular wall of polycystic ovary ( PCO ) in relation to the cystogenesis , we examined expression of lysyl oxidase ( LOX ) , which initiates cross link formation of the collagen and elastin in the extracellular matrix , and expression of matrix metalloproteinases ( MMPs ) in ovaries of model rats with PCO induced by dehydroepiandrosterone ( DHEA ) compared with MMP expression in control rats . ^^^ |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| We have studied the expression of lysyl oxidase ( LOX ) because it is the classical enzyme responsible for the cross linking of collagen and elastin . ^^^ |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| Lysyl oxidase catalyzes the final enzymatic step required for collagen and elastin cross linking in extracellular matrix biosynthesis . ^^^ |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| Senescent expression of genes coding tropoelastin , elastase , lysyl oxidase , and tissue inhibitors of metalloproteinases in rat vocal folds : comparison with skin and lungs . ^^^ Using a reverse transcriptase polymerase chain reaction ( RT PCR ) , mRNA levels of tropoelastin , elastase , lysyl oxidase , and tissue inhibitors of metalloproteinases ( TIMP 1 to TIMP 4 ) were measured in vocal folds , tail skin , and lungs of rats at ages 1 . 5 + / 0 . 5 weeks ( neonatal ) , 6 . 0 + / 0 . 5 months ( adult ) , and 24 + / 0 . 5 months ( elderly ) . ^^^ Vocal expression of both tropoelastin and lysyl oxidase peaks during the neonatal stage , followed by a significant decrease . ^^^ In summary , compared to what is found in adult rats , there is a significant up regulation in the expression of vocal elastase in the elderly animals in the presence of unchanged vocal expression of tropoelastin , lysyl oxidase , and TIMPs . ^^^ |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| The first step in normal cross linking in elastin is the formation of alpha aminoadipic delta semialdehyde , allysine , through oxidative deamination of specific peptidyl lysine by the enzyme lysyl oxidase ( EC 1 . 4 . 3 . 13 ) . ^^^ |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| Tropoelastin and lysyl oxidase mRNA expression , as assessed by Northern blot analysis , was also stimulated by GSNO . ^^^ In conclusion , NO seems to enhance aortic elasticity via tropoelastin and lysyl oxidase upregulation . . ^^^ |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| Crosslinking of rhTE into elastin and the accompanying formation of tritiated water was inhibited by beta aminoproprionitrile , a potent inhibitor of lysyl oxidase , an enzyme critical for the post translational processing of elastin and collagen . ^^^ We show for the first time that cells expressing lysyl oxidase , but not elastin , as well as elastogenic cells can incorporate rhTE into insoluble elastin with the formation of elastin crosslinks . ^^^ Incorporation of recombinant human tropoelastin into extracellular matrices using nonelastogenic rat 1 fibroblasts as a source for lysyl oxidase . ^^^ Acellular NNRSMC matrices were produced and replated with Rat 1 fibroblasts , cells that were found to express lysyl oxidase but not tropoelastin . ^^^ |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| Since abnormalities in connective tissue cross linking have been reported in ALS patients , an enzyme of possible relevance is lysyl oxidase ( LOX ) , a copper containing enzyme which catalyses the crosslinking of collagens and elastin . ^^^ |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| Lysyl oxidase catalyzes the final known enzymatic step required for collagen and elastin cross linking in the biosynthesis of normal mature functional insoluble extracellular matrices . ^^^ Lysyl oxidase activity in biological samples is traditionally and most reliably assessed by tritium release end point assays using radiolabeled collagen or elastin substrates involving laborious vacuum distillation of the released tritiated water . ^^^ |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| Cytokine regulation of gingival fibroblast lysyl oxidase , collagen , and elastin . ^^^ The present study investigates in cultured human gingival fibroblasts the regulation of lysyl oxidase , alpha 1 type 1 collagen , and elastin by selected cytokines that are elevated in drug induced gingival overgrowth tissues . ^^^ Cells were harvested at intervals , and changes in lysyl oxidase enzyme activity , and in mRNA levels of lysyl oxidase , alpha 1 type 1 collagen , and elastin were determined . ^^^ IL 1 , IL 6 , and PDGF BB did not significantly regulate lysyl oxidase enzyme activity , or alpha 1 type 1 collagen , elastin , and lysyl oxidase mRNA levels under the conditions tested . ^^^ |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| Lysyl oxidase , an extracellular amine oxidase , controls the maturation of collagen and elastin . ^^^ |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| METHODS AND RESULTS : By differential display analysis , we have studied the effect of high concentrations of native low density lipoprotein ( LDL ) on endothelial gene expression . mRNA levels of lysyl oxidase ( LOX ) , an enzyme involved in collagen and elastin cross linking , were downregulated by LDL treatment in endothelial cells in a dose and time dependent manner ( 80 % of inhibition by 180 mg / dL LDL for 24 hours ) . ^^^ |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| Lysyl oxidase ( LOX ) catalyzes the final enzymatic reaction required for cross linking of collagen and elastin fibers and therefore has a crucial role in regulating the formation and maintenance of extracellular matrix in the ovary . ^^^ |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| By mRNA differential display in mouse hearts , lysyl oxidase ( Lox ) , a key enzyme catalyzing cross links in elastin and collagens , was found to be up regulated between embryonic days 11 ( E 11 ) and 13 ( E 13 ) . ^^^ |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| We investigated the expression of major elastic fiber components such as tropoelastin ( TE ) and fibrillin 1 ( FBN 1 ) and elastin related enzyme , lysyl oxidase ( LO ) , in a calcification model using beta glycerophosphate ( beta GP ) in cultured bovine aortic smooth muscle cells ( BASMCs ) . ^^^ |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| Lysyl oxidase ( LO ) plays a critical role in the formation and repair of the extracellular matrix ( ECM ) by oxidizing lysine residues in elastin and collagen , thereby initiating the formation of covalent crosslinkages which stabilize these fibrous proteins . ^^^ |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| Lysyl oxidase ( LOX ) , originally known as the enzyme required for initiation of covalent cross linking in collagens and elastin , is now known to be a member of a family of genetically related proteins . ^^^ |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| Lysyl oxidase ( LOX ) is the enzyme that cross links extracellular collagen and tropoelastin and is involved in tumor suppressor activity . ^^^ |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| Lysyl oxidase catalyzes oxidative deamination of peptidyl lysine and hydroxylysine residues in collagens and lysine residues in elastin to form peptidyl aldehydes that are required for the formation of covalent cross links in normal extracellular matrix biosynthesis . ^^^ |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| Lysyl oxidase ( LO ) plays a central role in the crosslinking of collagen and elastin in the extracellular matrix . ^^^ |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| Lysyl oxidase ( LOX ) is an enzyme responsible for the cross linking of collagen and elastin both in vitro and in vivo . ^^^ |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| In this study , we utilized lysyl oxidase ( LO ) to enzymatically crosslink extracellular matrix ( ECM ) proteins , particularly collagen and elastin , to enhance the mechanical integrity of the ECM and thereby impart mechanical strength to the engineered tissue . ^^^ |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| There was a strong up regulation of lysyl oxidase expression during weeks 1 3 with a peak in expression at week 3 , correlating with the phases of collagen and tropoelastin production . ^^^ |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| Remarkably , the yeast PPLO is nearly as effective in oxidizing a mammalian tropoelastin substrate as is a true mammalian lysyl oxidase isolated from bovine aorta . ^^^ |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| Here we show that mice lacking the protein lysyl oxidase like 1 ( LOXL 1 ) do not deposit normal elastic fibers in the uterine tract post partum and develop pelvic organ prolapse , enlarged airspaces of the lung , loose skin and vascular abnormalities with concomitant tropoelastin accumulation . ^^^ |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| Elastic fiber formation involves the secretion of tropoelastin which is converted to insoluble elastin by cross linking , initiated by the oxidative deamination of lysine residues by lysyl oxidase . ^^^ |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| Lysyl oxidase , secreted by activated vascular smooth muscle cells and fibroblasts , catalyzes a key step in the cross linking and stabilization of collagen and elastin in the vascular wall . ^^^ This suggests that lysyl oxidase may have an important role in stabilization of collagen and elastin at sites of vascular injury and that modulation of lysyl oxidase activity may be a viable method to prevent or reduce recurrent stenosis . ^^^ This study focuses on lysyl oxidase , a key enzyme involved in stabilization of collagen and elastin . ^^^ |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| BACKGROUND : Lysyl oxidase is a promising candidate gene for a mutation search in intracranial aneurysm families because ( a ) it controls the processing , cross linking and maturation of collagen and elastin fibers in the blood vessel wall , ( b ) its expression levels and activity are altered in different animal models of aneurysm pathogenesis , and ( c ) it is encoded within the chromosome 5q22 31 region of suggestive linkage to intracranial aneurysms . ^^^ |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| Lysyl oxidase is an extracellular enzyme critical for the normal biosynthesis of collagens and elastin . ^^^ |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| At 50 or 100 ng / ml , rhFGF 18 increased the expression of alpha smooth muscle actin ( alpha SMA ; 2 . 5 fold ) , a characteristic marker of myofibroblasts , of tropoelastin ( 6 . 5 fold ) , of lysyl oxidase ( 2 fold ) , and of fibulins 1 and 5 ( 8 and 2 . 2 fold ) in confluent fibroblasts isolated from fetal day 21 lung ; similar results were obtained with fibroblasts from day 3 postnatal lungs . ^^^ |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| Lysyl oxidase ( LO ) , a copper dependent enzyme , plays a critical role in the formation and repair of the extracellular matrix ( ECM ) by catalyzing the crosslinking of elastin and collagen . ^^^ |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| In addition , these dysfunctions of elastin fiber are also associated , in that the down regulation of elastin and its related components ( fibrillin 1 and lysyl oxidase ) are directly related to calcification in SMCs . ^^^ |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| DPA may interfere with elastin cross linking through inhibition of the enzyme lysyl oxidase , or by formation of complexes with the cross linked precursors , impairing a normal maturation of elastic fibers . ^^^ |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| Lysyl oxidase ( LOX ) and lysyl oxidase like ( LOXL ) are extracellular enzymes that deaminate peptidyl lysyl residues involved in the cross linking of fibrillar collagens and elastin . ^^^ |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| This lactone specifically inhibits lysyl oxidase which depends on copper to catalyze cross linking of collagen and elastin in arteries and bone . ^^^ |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| Tropoelastin is encoded by a single human gene that spans 36 exons and is oxidized in vivo by mammalian lysyl oxidase at the epsilon amino group of available lysines to give the adipic semialdehyde , which then facilitates covalent cross link formation in an enzyme free process involving tropoelastin association . ^^^ We demonstrate here that this process is effectively modeled by a two protein component system using purified lysyl oxidase from the yeast Pichia pastoris to facilitate the oxidation and subsequent cross linking of recombinant human tropoelastin . ^^^ |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| BACKGROUND : Lysyl oxidase catalyzes a key step in the cross linking of collagen and elastin in the extracellular matrix . ^^^ |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| Mammalian lysyl oxidase ( LOX ) is essential for the catalysis of lysyl derived cross links in fibrillar collagens and elastin in the extracellular matrix and has also been implicated in cell motility , differentiation , and tumor cell invasion . ^^^ |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| Lysyl oxidase ( LOX ) is a copper containing amine oxidase known to catalyze the covalent cross linking of fibrillar collagens and elastin at peptidyl lysine residues . ^^^ |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| RESULTS : We confirmed that ARPE 19 cells express fibrillin containing microfibrils and lysyl oxidase , but they do not express tropoelastin . ^^^ |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| Lysyl oxidase ( LO ) catalyzes crosslinking of collagen and elastin essential for maintaining the structural integrity of the lung extracellular matrix ( ECM ) . ^^^ |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| Lysyl oxidase is the enzyme that is essential for collagen and elastin cross linking . ^^^ |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| An initial gene array analysis followed by real time qPCR revealed that the message levels for tropoelastin and lysyl oxidase were as high as 8 fold in SCI rats compared to normal . ^^^ |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| Lysyl oxidase ( LOX ) , an extracellular enzyme , plays a key role in the post translational modification of collagens and elastin , catalyzing inter and intra crosslinking reactions . ^^^ |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| Severe emphysema , presumably caused by abnormal elastin due to deficiency of the copper dependent enzyme lysyl oxidase , may represent an underestimated clinical complication of Menkes disease and should be considered in the differential diagnosis of chronic respiratory disease in these patients . . ^^^ |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| Lysyl oxidase ( Lox ) is a copper dependent amine oxidase that catalyzes the cross linking of collagen and elastin fibers in the extracellular matrix ( ECM ) . ^^^ |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| Copper ( Cu ) dependent lysyl oxidase ( LO ) catalyzes crosslinking of collagen and elastin stabilizing the extracellular matrix ( ECM ) . ^^^ |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| However , expression of tropoelastin or lysyl oxidase mRNA was unaffected in fibulin 4 / mice . ^^^ |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| Lysyl oxidase initiates the enzymatic stage of collagen and elastin cross linking . ^^^ |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| In this study , we aimed to increase crosslinking in collagen and elastin in the extracellular matrix through overexpression of lysyl oxidase ( LO ) in order to improve mechanical strength in dermal wounds during healing . ^^^ |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| Elastin , tenascin C , thrombospondin , lysyl oxidase , and osteopontin are expressed within the wall of the remodeling vein resulting in the de novo formation of an extracellular matrix scaffold that may be part of a critical adaptation program being evoked to allow the vessel to cope with its new biomechanical environment . ^^^ |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| BACKGROUND : Lysyl oxidase cross links collagen and elastin . ^^^ |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| LOX enzyme is known to downregulate p 21 ( ras ) protein and play a central role in the maturation of collagen and elastin in the extracellular matrix as well as modulate the cytoskeletal elements . ^^^ |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| LOX has been traditionally known for one function , the extracellular catalysis of lysine derived cross links in fibrillar collagens and elastin . ^^^ |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| Pigs finished in the lOx pens were fatter ( P < 0 . 05 ) at the last lumbar vertebra than pigs finished in the CONT pens , but no significant differences were found in loineye area , loin color , marbling scores , WBS , sensory panel scores , retail display measures , or muscle fiber type percentages . ^^^ |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| Multiple regression analysis showed that elastic lamellar thickness ( ELT ) was correlated positively with the SSAO : elastin ratio and negatively with the Lox : elastin ratio , and that the clinical features of IAAED ( aneurysm , thoracic aorta diameter , and aortic insufficiency ) were positively correlated with ELT but not with SSAO . ^^^ |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| In addition to collagen and elastin cross linkages , regulatory and activation mechanisms and cell type specific LOX interactions may contribute to a range of novel intra and extracellular LOX functions that appear critical determinants of the cellular microenvironment in the normal skin and in these skin disorders . . ^^^ |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| Lysyl oxidases , a family comprising LOX and four LOX like enzymes , catalyze crosslinking of elastin and collagens . ^^^ |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| Ligand blot and mammalian two hybrid assays confirmed an interaction between tropoelastin and the pro regions of both LOX and LOXL . ^^^ Our results showed that the pro regions of LOX and LOXL play a significant role in directing the deposition of both enzymes onto elastic fibers by mediating interactions with tropoelastin . ^^^ |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P28300 and P15502 |
Pubmed |
SVM Score :0.0 |
| NA |
|