| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.91841844 |
| A progress curve kinetic method was developed to investigate the interaction between human leukocyte elastase and macromolecular substrates , such as insoluble elastin and soluble plasma proteins . 0.91841844^^^ |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.66107936 |
| Elastin from human aorta was incubated with human neutrophil elastase under conditions favoring proteolysis . 0.66107936^^^ |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :1.5348705 |
| To understand the interaction between elastin and elastase , elastin from human aorta was incubated with human leukocyte elastase under conditions favoring proteolysis . 1.5348705^^^ |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.90053516 |
| Our studies suggest that interaction of HLE with elastin in vivo may be very persistent and permit progressive solubilization of this structurally important extracellular matrix component . . 0.90053516^^^ |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| Degradation of tropoelastin and elastin substrates by human neutrophil elastase , free and bound to alpha 2 macroglobulin in serum of the M and Z ( Pi ) phenotypes for alpha 1 antitrypsin . ^^^ Human neutrophil elastase degraded tropoelastin approximately 9 times faster than it did solubilized elastin and approximately 19 times faster than it did lung elastin . ^^^ |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| The leukocyte elastase inhibitory activity of AA 231 1 , a suicide substrate , was investigated in the presence of elastin , a natural substrate of elastase , and its efficiency to reduce the degradation of basement membrane and haemorrhage induced by elastase was analysed . ^^^ |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| NE and PR 3 assist in the destruction of phagocytosed microorganisms , cleave the important connective tissue protein elastin , and generate chemotactic activities by forming alpha 1 proteinase inhibitor complexes and elastin peptides . ^^^ |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| The elastolytic activity of Pseudomonas aeruginosa elastase ( MW = 39K ) and human neutrophil elastase ( MW = 33K ) were also examined using insoluble bovine neck and lung elastin . ^^^ |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| SR 26831 inhibited in a dose dependent manner degradation of [ 3H ] elastin and [ 3H ] collagens ( types 1 and 4 ) by human leukocyte elastase ( IC 50 values were between 1 . 2 and 1 . 8 microM ) . ^^^ |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| On a molar basis the recombinant 92 kDa type 4 collagenase was approximately 30 % as active as human leukocyte elastase in solubilizing 3H labeled elastin . ^^^ |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| We investigated the effect of SSP on elastin degradation by porcine pancreatic elastase ( PE ) and crude extract of human neutrophil elastase ( NE ) in solution and gel containing alpha 1PI . ^^^ SSP having no elastase activity enhanced PE and NE induced elastinolysis in solution when added to alpha 1PI before mixing with elastase and then with elastin . ^^^ |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| Several pentacyclic triterpenoid metabolites of plant origin are inhibitors of hydrolysis of both synthetic peptide substrates and elastin by human leucocyte elastase ( HLE ) . ^^^ |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| The compound effectively inhibited HNE hydrolysis of bovine ligamentum nuchae elastin . ^^^ |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| The compound effectively inhibited hydrolysis of bovine ligamentum nuchae elastin by HNE . ^^^ |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| In vitro , coincubation of trypsin with PPE resulted in a slight enhancement of rate of hydrolysis of elastin , while coincubation of trypsin with HLE resulted in a significant reduction of the rate of hydrolysis . ^^^ |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| Elafin , a human skin derived inhibitor of human leukocyte elastase , was tested for inhibitory activity against proteinase 3 , an elastin degrading proteinase of neutrophils . ^^^ |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| Gel filtration of elastin digests prepared by hydrolysis of human lung elastin using a low ( 1 : 500 ) ratio of neutrophil elastase to elastin generated a broad protein peak of approximately 70 , 000 daltons . ^^^ In contrast , a high ( 1 : 25 ) ratio of neutrophil elastase to human lung elastin gave a broad protein peak , with a size distribution in the 10 , 000 to 30 , 000 dalton range . ^^^ |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| Elastin decreases the efficiency of neutrophil elastase inhibitors . ^^^ |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| A great deal of experimental evidence suggests that emphysema in smokers is caused by the action of neutrophil elastase on lung elastin . ^^^ |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| ICI 200 , 355 also inhibited the ongoing destruction of insoluble elastin by human neutrophil elastase . ^^^ |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| Its effect on two types of leukocyte elastase , purified human leukocyte elastase and elastase freshly discharged upon stimulation of guinea pig polymorphonuclear neutrophils , was examined using three methods : the cleavage of a chromogenic peptide substrate , MeO Suc Ala Ala Pro Val NA , the lysis and solubilization of tritiated elastin and the microscopic examination of the damage to lung elastic network . ^^^ |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| This test makes use of human aorta elastin hydrolyzed by a chemical procedure ( kappa elastin ) instead of EP produced by pancreatic or leukocyte elastase . ^^^ |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| Human leukocyte elastase ( HLE ) hydrolysed baboon aortic elastin 8 times faster than human cathepsin G . ^^^ The kinetic constants 5 and [ S 50 ] of aortic elastin hydrolysis by HLE ( 0 . 15 microM ) were 0 . 00286 mg 10 ml 1 10 min 1 and 0 . 158 mg 10 ml 1 , respectively . ^^^ One mg of this elastin could be saturated with 5 . 6 micrograms of HLE . ^^^ As with elastins isolated from other sources , the hydrolysis of baboon aortic elastin by HLE was highly sensitive to ionic strength , and a biphasic effect was obtained with increasing NaCl concentrations . ^^^ Cathepsin G , but not bovine alpha chymotrypsin , was able to stimulate the rate of hydrolysis of baboon aortic elastin by HLE . ^^^ |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| Modifications in plasma elastin peptides and neutrophil elastase generated fibrinopeptide A , urinary desmosines , and bronchoalveolar lavage fluid neutrophils or neutrophil elastase were the indicators of success or failure of the treatment . ^^^ |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| Using elastin Congo Red as substrate , CT inhibited EIC against MLE and PPE by approximately 70 % but did not affect the EIC against HLE . ^^^ |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| MR 889 also inhibited the elastolytic activity of leukocyte elastase , as determined with insoluble elastin as the substrate . . ^^^ |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| In vitro studies in our laboratory have shown that HLE elastin complexes may be remarkably stable . ^^^ Further , we found evidence for two distinct mechanisms of inhibition of elastin bound HLE . alpha 1PI and eglin C prevented elastin solubilization largely by enhancing net dissociation of HLE from the complexes ; enzyme remaining bound to the substrate retained essentially full activity . ^^^ Inhibition of human leukocyte elastase bound to elastin : relative ineffectiveness and two mechanisms of inhibitory activity . ^^^ |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| Inhibition of human pancreatic elastase 2 activity on human aortic elastin by human alpha 2 macroglobulin . ^^^ Human alpha 2 macroglobulin human pancreatic elastase 2 binding were investigated using a homologous substrate , human aortic elastin , in order to test the enzymatic activity . ^^^ |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| We have determined the effect of two elastase specific synthetic low molecular weight substrates , L pyroglutamylprolylvaline paranitroanilide and succinyltrialanyl paranitroanilide , together with insoluble elastin fluorescein , on the determination of the neutrophil elastase ( NE ) inhibitory capacity of purified alpha 1 proteinase inhibitor ( alpha 1 PI ) and bronchial antileukoprotease ( ALP ) . ^^^ |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| Elastase 2 with the chymotrypsin substrates and with elastin . ^^^ |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| That this activity was due to alpha 2 macroglobulin ( A2MG ) complexed neutrophil elastase was evidenced by ( a ) the Sephadex G 75 elution profile ; ( b ) the inactivity against insoluble [ 3H ] elastin ; ( c ) the inhibitory profile with phenylmethylsulfonyl fluoride , methoxy succinyl alanyl alanyl prolyl valyl chloromethylketone , ethylene diamine tetraacetic acid , and A1AT ; and ( d ) the immobilization by A2MG antibody bound to polystyrene plates . ^^^ |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| The major physiological role of this inhibitor is to protect elastin fibers in the alveolar structure of the lung from excessive degradation by neutrophil elastase . ^^^ |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| This study was conducted to determine UA collagen and elastin content , diameter , alpha 1 and alpha 2 adrenergic receptor ( AR ) numbers , norepinephrine ( NE ) and in vitro reactivity to phenylephrine on d 0 , 20 , 50 , 80 and 110 of pregnancy in the pig . ^^^ |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| Neutrophil cytoplasts , which produced O 2 and H2O2 but lacked azurophilic granules , did not significantly enhance either elastin or collagen degradation by purified neutrophil elastase ( NE ) . ( ABSTRACT TRUNCATED AT 250 WORDS ) . ^^^ |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| We also used bovine ligamentum nuchae elastin and human leukocyte elastase for comparative purposes . ^^^ In addition , the bacterial enzyme is about 10 times more active on human elastin than the neutrophil elastase but the latter is about 5 times more active on bovine elastin than the former . ^^^ |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| Its ability to degrade elastin at pH 6 . 5 is slightly greater than that of HLE , but it is less active than HLE at pH 7 . 4 or 8 . 9 . ^^^ |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| The conductimetric method was applied to the measurement of human leukocyte elastase activity , using insoluble elastin as a substrate . ^^^ |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| Semisynthetic inhibitors of human leukocyte elastase and their protective effect on lung elastin degradation in vitro . ^^^ |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| Poly G inhibits elastin proteolysis even when subsequently added to mixtures of elastin and HNE that have first been preincubated together for 10 min . ^^^ |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| In the present study , the effects of AP on human neutrophil elastase and Pseudomonas elastase were investigated , and AP was shown to interfere with the cleavage of soluble elastin . ^^^ |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| Based on available knowledge , this study shows that alpha 1 proteinase inhibitor ( alpha 1 PI ) plays an important role in protecting lung elastin from elastolytic proteinases , particularly human neutrophil elastase ( HNE ) . ^^^ |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| One of these enzymes , a cysteine proteinase , after purification to homogeneity was found to degrade insoluble bovine lung elastin at a rate comparable to human neutrophil elastase . ^^^ |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| KM and Vmax values derived from Lineweaver Burk plots indicate that , if a near uniformity exists in KM , differences were observed in catalytic rates , kcat increasing approximately 40 % for copper deficient elastin elastolysis by leukocyte elastase . ^^^ |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| These inhibitors function to limit the activity of serine proteases in vivo . alpha 1 PI acts as an inhibitor of neutrophil elastase to protect the elastin fibers of the lung . ^^^ |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| There was a correlation between a quantitative measure of the local distribution of neutrophil elastase in contact with alveolar interstitial elastin and the local presence of emphysematous change as determined by mean linear intercept of the various histologic sections . ^^^ |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| Several laboratories , including our own have reported the synthesis and activity of certain low relative molecular mass inhibitors of mammalian serine proteases , especially human leukocyte elastase ( HLE , EC 3 . 4 . 21 . 37 ) , an enzyme whose degradative activity on lung elastin has been implicated as a major causative factor in the induction of pulmonary emphysema , and which is present in the azurophil granules of human polymorphonuclear leukocytes ( PMN ) . ^^^ Under certain pathological conditions , however , PMN become attached to host protein ( elastin fibres , basement membrane , connective tissue , immune complexes ) , and in response to this adherence , the granules may fuse with the PMN outer membrane and release their contents , including HLE , directly onto the tissue . ^^^ |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| Increasing the concentration of the synthetic substrate Suc [ Ala ] 3 pNA in the assay from 0 . 45 mmol / l to 7 . 2 mmol / l reduced the observed inhibitory capacity by 53 % and the use of elastin fluorescein gave lower results for inhibitory capacity than the Suc [ Ala ] 3 pNA ( median 0 . 26 mol neutrophil elastase / mol measured inhibitors ( range 0 0 . 72 ) with elastin ; 1 . 40 mol neutrophil elastase / mol measured inhibitors ( 0 . 80 3 . 21 ) with Suc [ Ala ] 3 pNA ) . ^^^ |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| For the solubilization of elastin by HLE by 1 I . ^^^ |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| To study the susceptibility to solubilization of insoluble elastin , cultures were incubated in the presence of human neutrophil elastase ( HNE ) or porcine pancreatic elastase ( PPE ) and in the absence of serum for periods up to 45 min . ^^^ Although HNE and PPE exhibited comparable activity against elastin purified from the cell layer , HNE exhibited a 6 . 7 to 25 fold reduction in its elastin solubilizing activity using intact cell layers as compared with the purified elastin , whereas PPE exhibited only a 1 . 5 to 2 . 5 fold reduction . ^^^ The mean elastin content of PPE solubilized protein was 110 % that of the elastin content of the corresponding cell layer ; the value for HNE solubilized protein was only 16 % . ^^^ Thus , the amount of elastin per microgram of solubilized protein for HNE was 15 % that for PPE . ^^^ Possible explanations for the greatly diminished elastolytic activity of HNE in the culture system include the preference of HNE for other substrates in the cell layer , the inability of HNE to penetrate sufficiently into the cell layer , and the presence of sulfated glycosaminoglycans in the vicinity of the elastin that act in an inhibitory fashion . ^^^ |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| Digestion of elastin by porcine pancreatic elastase 1 and elastase 2 . ^^^ Elastin was fully solubilized by digestion with elastase 1 or elastase 2 . ^^^ |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| These results were not consonant with a report that Cat G and HNE were synergistic in solubilizing human lung elastin . ^^^ The combination of Cat G and HNE did not solubilize significantly more hamster lung elastin ( 23 % ) than the sum of their individual activities . ( ABSTRACT TRUNCATED AT 250 WORDS ) . ^^^ |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| Although alpha 1 proteinase inhibitor ( alpha 1 antitrypsin ) is widely thought to protect lung elastin against the elastolytic action of leukocyte elastase , there is only circumstantial evidence for such a protective role . ^^^ |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| These elastin preparations , when utilized as substrates for various mammalian proteinases , were solubilized by porcine elastase at a rate six times faster than human leukocyte elastase . ^^^ Leukocyte cathepsin G also solubilized lung elastin but only at 12 % of the rate of the leukocyte elastase . ^^^ However , inhibition of leukocyte elastase by alpha 1 proteinase inhibitor was significantly suppressed if the enzyme was preincubated with elastin prior to addition of the inhibitor . . ^^^ |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| Blood monocytes are progenitors of AM , and if they possess receptors for NE , they could bind and internalize NE in the pulmonary interstitium and may effect elastin degradation , which likely accompanies the development of emphysema . ^^^ Approximately 50 % of the 1 NE initially bound to either monocytes or AM remained cell associated at the end of culture ; 62 to 65 % of this material eluted at 29 , 000 daltons and solubilized particulate elastin . ( ABSTRACT TRUNCATED AT 250 WORDS ) . ^^^ |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| Regulation of elastolysis of insoluble elastin by human leukocyte elastase : stimulation by lysine rich ligands , anionic detergents , and ionic strength . ^^^ |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| Recent studies have focused on the possible role of elastolytic enzymes , particularly leukocyte elastase , in the pathogenesis of chronic obstructive lung disease , but there are no reports in the literature concerning the antigenic properties of peptides released by digestion of insoluble elastin with leukocyte elastase . ^^^ In the present study , we have obtained antibodies in rabbits to peptides prepared by digestion of dog and human lung and aortic elastin with either oxalic acid or leukocyte elastase . ^^^ |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| The remainder of the radiolabel eluted at 29 , 000 daltons , in fractions containing enzyme that solubilized particulate elastin , and likely represented intact neutrophil elastase . ^^^ |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| Purification of human leukocyte elastase and cathepsin G by chromatography on immobilized elastin . ^^^ Human leukocyte elastase and cathepsin G were isolated from purulent sputum by a simple procedure involving chromatography on elastin agarose . ^^^ Human leukocyte elastase and cathepsin G were isolated from purulent sputum by a simple procedure involving chromatography on elastin agarose . ^^^ |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| The ability of human AM to modulate NE mediated degradation of elastin and collagen in the surrounding matrix was studied to gain insights into the inflammatory process that accompanies the pathogenesis of emphysema in humans . ^^^ When 14 10 10 ( 6 ) AM were added to the extracellular matrix , followed 2 h later by addition of 2 micrograms of NE , collagen and elastin degradation measured at 24 h were not significantly different from that which occurred when matrix was incubated with NE in the absence of AM . ^^^ |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| Effect of neutrophil cathepsin G on elastin degradation by neutrophil elastase . ^^^ Human neutrophil cathepsin G was found to be unable to significantly stimulate the degradation of either bovine or human elastin by neutrophil elastase , using four different procedures to monitor digestion . ^^^ |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| The quantitative adsorption of pancreatic elastase was similar on either untreated or PCO treated elastin suggesting that the binding of this compound to elastin increases the non productive catalytic sites of elastase molecules . ( + ) Catechin insoluble elastin complexes were partially resistant to the degradation induced by human leukocyte elastase but were hydrolysed at the same rate as untreated samples by a constant amount of pancreatic elastase . ^^^ |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| The pH profile of elastin degradation by neutrophil extracts generally followed that of purified human leukocyte elastase . ^^^ |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| The P . aeruginosa elastase also freed active PMN elastase from complexes with BMI , resulting in elastin digestion by both enzymes . ^^^ |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| Measurements using insoluble elastin labeled with 3H demonstrated that smokers ' macrophages also contained a serine proteinase activity whose inhibitor profile resembled that of polymorphonuclear leukocyte elastase . ^^^ |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| Elastin derived peptides , produced by digesting human aortic elastin and bovine ligament elastin with human neutrophil elastase , were tested for chemotactic activity . ^^^ |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| Evidence that human leukocyte elastase is selective for cross linked regions of elastin . ^^^ |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| Human leukocyte cathespin G strongly stimulates the rate of solubilization of human lung elastin by human leukocyte elastase . ^^^ Potentiation of leukocyte elastase digestion of lung elastin may also be brought about by bovine alpha chymotrypsin . ^^^ Stimulation of leukocyte elastase activity by cathepsin G is about 3 times less pronounced with bovine ligamentum nuchae elastin than with human lung elastin . ^^^ Therefore , maximal potentiation of elastolysis occurs with the lung elastin / leukocyte elastase system . ^^^ |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| Effect of different elastase inhibitors on leukocyte elastase pre adsorbed to elastin . ^^^ |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| In addition , aqueous solutions of unfractionated cigarette smoke were incubated with leukocyte elastase or serum , and the abilities of the smoke treated enzyme to digest elastin and of the smoke treated serum to inhibit elastin digestion were determined . ^^^ |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| Bovine ligament tropoelastin and elastin derived peptides , generated from either human aortic elastin with human neutrophil elastase or from bovine ligament elastin with pancreatic elastase , elicited chemotactic responses that were maximal at 0 . 2 micrograms / ml ( 3 10 10 ( 9 ) M ) and 0 . 5 2 . 0 micrograms protein / ml , respectively . ^^^ |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| The stimulatory effect of PAI 1 on HLE activity was not restricted to the low molecular weight pNA substrate but was also revealed using a natural substrate assay ( bovine neck ligament elastin solubilization ) . ^^^ |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| Although both enzymes solubilize elastin purified from these cultures at similar rates , PPE solubilized 11 . 5 times more elastin from the intact cultures than did HNE . ^^^ Because the amount of elastin in these cultures increases with increasing distance from the free surface , the lesser amounts of elastin solubilized by HNE may be partly due to poor penetration of HNE into the living extracellular matrix , resulting in limited access to elastin substrate . ^^^ Ultrastructural and immunocytochemical studies indicated , however , that even when HNE does have access to elastin substrate , it is less efficient than PPE at penetrating and degrading individual elastic fibers . . ^^^ |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| CONCLUSIONS : A deficiency of alpha 1 AT may allow proteases such as neutrophil elastase to destroy dermal elastin and , thus , produce cutis laxa in Marshall ' s syndrome . ^^^ |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| The inhibitory activity of ( Asn 55 Ala107 ) SLPI against the proteolysis of the natural substrates elastin and collagen by NE was comparable with that of full SLPI ( elastin , IC 50 = 907 + / 31 nM for SLPI , 767 + / 33 nM for ( Asn 55 Ala107 ) SLPI ; collagen , IC 50 = 862 + / 36 nM for SLPI , 727 + / 47 nM for ( Asn 55 Ala107 ) SLPI ) . 4 . ^^^ |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| Neutrophil elastase burden and elastin loss occur without alteration in the bronchoalveolar lavage cell population . ^^^ |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| The classical Bowman Birk inhibitor from soya retards strongly the hydrolysis of elastin catalyzed by leukocyte elastase , cathepsin G and a mixture of both . ^^^ |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| Elastin derived peptides protect elastic fibres degradation by human neutrophil elastase : in vitro and in vivo studies using a mechanically induced rat gingival inflammatory model . ^^^ Without influencing leucocyte infiltration , it is proposed that elastin derived peptides , acting as potent competitive inhibitor of neutrophil elastase involved in periodontitis , might be of therapeutic value . . ^^^ |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| P monocytes constitute 20 30 % of the circulating monocyte pool and are characterized by 1 ) avid adherence to extracellular matrix through high level cell surface expression of alpha 5 , beta 1 , and beta 2 integrins ; 2 ) high capacity to produce reactive oxygen species ; 3 ) high content of serine proteinases and alpha 1 proteinase inhibitor ; and 4 ) proteolytic activity against a soluble peptide human leukocyte elastase substrate , [ 3H ] elastin , and solid phase fibronectin , even in the presence of proteinase inhibitors . ^^^ |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| Inhibition of elastin hydrolysis , catalyzed by human leukocyte elastase and cathepsin G , by the Bowman Birk type soy inhibitor ] . ^^^ Cathepsin G stimulates the hydrolysis of elastin from bovine neck ligament catalyzed by human leukocyte elastase . ^^^ The classical Bowman Birk inhibitor from soya retards strongly the hydrolysis of elastin catalyzed by leukocyte elastase , cathepsin G and the mixture of both . ^^^ |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| HLE is capable of cleaving many proteins , including elastin , other components of connective tissue , certain complement proteins , and receptors . ^^^ |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| We synthesized N oleoyl heparin derivative ( 3 oleoyl groups / one molecule of heparin ) ; such a lipophilic glycosaminoglycan ( LipoGAG ) , although acting as an elastin protecting agent , possessed lower HNE inhibitory capacity as compared with heparin . ^^^ |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| Human neutrophil elastase ( HNE ) digested the remaining insoluble elastin samples 4 . 3 , 6 . 0 , and 9 . 8 times faster than unexposed elastin . ^^^ In contrast , NO 2 exposed elastin was no more susceptible to digestion by HNE . ^^^ |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| Proteinase 3 and NE contribute to the destruction and elimination of microorganisms , cleave elastin and other proteins of connective tissues , and generate chemotactic activities by forming alpha 1 proteinase inhibitor ( alpha 1 PI ) complexes . ^^^ |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| Selected compounds efficiently inhibited the degradation of insoluble bovine neck elastin by HLE in a concentration dependent manner . ^^^ |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| HNE mediated degradation of proteoglycan and elastin in vitro was inhibited by MDL 101 , 146 in a dose related manner . ^^^ |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| The present study investigated whether neutrophils in patients with AAA actively synthesize the neutrophil elastase enzyme and the effect of elastin derived peptides on neutrophil elastase release . ^^^ |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| In contrast , HLE hydrolyzed elastin approximately 22 fold faster than HCG , but was only slightly more rapid than HCG when [ 3H ] transferrin was used as substrate . ^^^ |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| Inhibition of elastin hydrolysis , catalyzed by leukocyte elastase ] . ^^^ The soybean Bowman Birk type inhibitor effectively inhibited the elastin hydrolysis by leukocyte elastase both when the enzyme and the inhibitor were simultaneously added to the substrate and after preliminary elastase adsorption on elastin . ^^^ |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| Although both enzymes solubilized protein at similar rates , PPE solubilized elastin five times faster than did HNE . ^^^ |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| The following biomarkers have been evaluated : cellularity of sputum , elementary analysis of particles in TEM / EDAX , plasma neutral metalloendo peptidase elastase type ( NMEP ) , leucocyte elastase ( HLE ) , fibronectin ( FN ) and elastin peptides . ^^^ |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| When elastinolytic activity of medullasin and human leukocyte elastase was determined by employing unstained elastin fibers and measuring the increase in 280 nm absorbance of the supernatant , elastinolytic activity amounting to several percent of that of porcine pancreas elastase was apparently observed . ^^^ These results indicate that medullasin and human leukocyte elastase are essentially devoid of elastinolytic activity , and that apparent elastinolytic activity observed when unstained elastin fibers were employed as the substrate is due to the digestion of collagen fibers mingled with elastin preparations . . ^^^ |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| Elastin can impair the human neutrophil elastase ( HNE ) inhibitory capacity of elastase inhibitors . ^^^ We synthesized oleoyl alanyl alanyl prolyl valine ( Ol Ala Ala Pro Val OH ) ( oleoyl peptide ) and the amides ( NH 2 and NH C3H7 ) of this peptide and studied their HNE inhibitory potencies using succinyl alanyl alanyl alanine p nitroanilide ( Suc Ala Ala Ala pNA ) or 3H labeled elastin as substrates , as well as cryostat sections of rabbit skin as an ex vivo substrate . ^^^ When the COOH terminal of the oleoyl peptide was derivatized to amide forms , the compound lost its ability to interact with HNE while keeping its elastin protecting function : IC 50 values for NH 2 and NH C3H7 derivatives were 22 and 17 microM , respectively . ^^^ Also , the HNE inhibitory capacity of Ol Ala Ala Pro Val OH was only reduced 2 fold by using elastin as a substrate . ^^^ This decrease was much lower than those determined with other HNE inhibitors of similar potency and could be accounted for by the ability of oleoyl peptide to bind to elastin . ^^^ |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| The enzymatic breakdown of human lung elastin by leukocyte elastase precedes the development of pulmonary emphysema , and releases elastin derived peptides ( EDPs ) into the circulation . ^^^ In the present study elastin was purified from human lung by sequential biochemical extraction , and solubilised by leukocyte elastase . ^^^ |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| Release of elastin peptides into lung lymph during CAE and attenuation of CAE induced pulmonary hypertension by neutrophil depletion suggest that neutrophil elastase may contribute to these changes . ^^^ |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| Lysozyme had no elastolytic activity as assessed by a standard tritium release assay , but , importantly , prevented the proteolytic degradation of elastin by human leukocyte elastase , pancreatic elastase , thermolysin , and Pseudomonas elastase . ^^^ |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| To evaluate effects of current smoking on the breakdown of lung elastin , we measured levels of elastin derived peptides ( EDP ) in unconcentrated bronchoalveolar lavage ( BAL ) fluid from 42 community based older asymptomatic volunteers ( 60 + / 11 yr [ mean + / SD ] ) and examined the relationships of the concentrations of EDP with immunologically detected neutrophil elastase ( NE ) bound with alpha ( 1 ) proteinase inhibitor and with esterolytic activity against the NE sensitive synthetic substrate , methoxysuccinyl alanyl alanyl prolyl valyl paranitroanilide ( MEOSAAPVNA ) . ^^^ These data provide further evidence that the concentrations of EDP increase in BAL fluid from current smokers compared with noncurrent smokers and that enhanced breakdown of the lung elastin is associated with the increased load of NE in the lung . . ^^^ Elastin derived peptides and neutrophil elastase in bronchoalveolar lavage fluid . ^^^ |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| Because NE is capable of destroying a broad range of substrates including cross linked elastin and the major forms of collagen as well as the cell walls of gram negative bacilli , it possesses the two edged sword property that is required for normal tissue turnover and host defense , yet potentially harmful in its ability to destroy normal tissues simultaneously . ^^^ |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| Inhibitor 1 ( 22 microM ) , 2 ( 50 microM ) or 3 ( 35 and 70 microM ) significantly reduced the HNE induced degradation of the elastin network by 75 , 42 , 54 and 44 % , respectively . ^^^ |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| In the presence of elastin peptides lymphocytes show increased proliferation and increased production of an elastase type serine protease apparently identical to PMN elastase , inhibited by cycloheximide and by anti PMN elastase antibodies . ^^^ |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| After isolation , purification , and radiolabeling of elastin from baboon aorta and lung , the rates of hydrolysis of both 3H labeled elastins by porcine pancreatic elastase ( PPE or by human leukocyte elastase ( HLE ) were compared . ^^^ In contrast , the low rate of hydrolysis of lung elastin ( 10 micrograms / h ) by HLE was paradoxically accompanied with a fivefold decrease in the Michaelis constant value and became negligible after 1 h of incubation . ^^^ Moreover , HLE adsorption isotherms showed that 0 . 87 nmol HLE was adsorbed on 1 mg of aorta elastin vs . 1 . 30 nmol / mg lung elastin . ^^^ Also , increasing ionic strength was found to enhance the elastolytic potential of HLE toward lung elastin . ^^^ Investigations were carried out to explain why baboon lung elastin exhibited low susceptibility to hydrolysis by HLE . ^^^ |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| CONCLUSION : Degradation of native elastin by leukocyte elastase can provide elastin derived peptides that act as autoimmune targets for T cells in GCA . . ^^^ |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| OBJECTIVE : Neutrophil elastase ( NE ) is the only neutral protease that is able to degrade insoluble elastin and other extracellular matrix constituents , and thus , may be involved in tumor invasion and metastasis . ^^^ |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| On a molar basis , rHME is approximately 30 % as active as human leukocyte elastase in solubilizing elastin . rHME also efficiently degrades alpha 1 antitrypsin ( alpha 1 AT ) , the primary physiological inhibitor of human leukocyte elastase . ^^^ |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| Once bound to insoluble elastin , 92 kDa progelatinase was totally unaffected by any potential activators tested ( tissue kallikrein , neutrophil elastase , plasmin , and stromelysin 1 ) , except aminophenylmercuric acetate ( APMA ) . ^^^ |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| The amino acid residues detected in insoluble elastin following hydrolysis with porcine pancreatic elastase and human neutrophil elastase were predominantly Gly and Ala , with lesser amounts of Val , Phe , Ile , and Leu . ^^^ |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| FK 706 also effectively inhibited the hydrolysis of bovine neck ligament elastin ( 2 mg / ml final concentration ) by human neutrophil elastase ( 4 microg / ml final concentration ) with an IC 50 value of 230 nM . ^^^ |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| When neutrophil elastase is already attached to the elastin fibres the smaller molecules SLPI and elafin appear to be better inhibitors of this enzyme than larger inhibitors such as A1AT and HEI . ^^^ |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| FR 134043 also inhibited hydrolysis of bovine neck ligament elastin by human neutrophil elastase with an IC 50 value of 210 nM . ^^^ |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| With reference to extracellular matrix components , the A . fumigatus serine proteinase was shown to degrade human lung elastin at a higher rate than an equimolar amount of human neutrophil elastase . ^^^ |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| Proteinase 3 ( PR 3 ) and neutrophil elastase ( NE ) are polymorphonuclear leukocyte serine proteinases that degrade extracellular matrix proteins including elastin and appear to be involved in the pathogenesis of several diseases characterized by tissue destruction most notably emphysema and Wegener ' s granulomatosis . ^^^ |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| Elastin substrate zymography revealed two phenylmethylsulfonyl fluoride inhibitable elastin degrading enzyme activities in the aortic tissue homogenate that corresponded to human neutrophil elastase ( approximately 30 kDa ) and its stable complex with alpha 1 proteinase inhibitor ( approximately 80 kDa ) , but no activity attributable to Pseudomonas elastase , a 33 kDa metal dependent enzyme . ^^^ |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| Its role is the inactivation of excessive neutrophil elastase in the pulmonary parenchyma , which is liberated during inflammation and destroys elastin and other components of extra cellular connective tissue matrix . ^^^ |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| METHODS : Elastin degradation by HNE was monitored spectrophotometrically with elastin congo red . ^^^ RESULTS : FR 901277 inhibited HNE induced elastin degradation . ^^^ OBJECTIVE AND DESIGN : A neutrophil elastase inhibitor FR 901277 was examined for its inhibitory effect on degradation of natural substrate elastin in vitro , and on acute inflammatory states and pulmonary emphysema in vivo . ^^^ |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| Besides having direct effects on structural elastin , NE stimulates the release of proinflammatory mediators from the respiratory epithelium and is a potent secretogogue . ^^^ |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| Human neutrophil elastase ( HNE ) , an enzyme secreted by activated neutrophils , can bind to and degrade extracellular matrix including human lung elastin . ^^^ In this study , we hypothesized that an alveolar macrophage ( AM ) product or products may interact with neutrophil elastase ( NE ) and modulate its binding to elastin . ^^^ In conclusion , macrophages release a factor or factors , probably lipid , which can interact with NE and inhibit its binding to human lung elastin without inhibiting elastase activity . ^^^ |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| We have established that treatment of cultured human skin fibroblasts with tropoelastin or with heterogenic peptides , obtained after organo alkaline or leukocyte elastase hydrolysis of insoluble elastin , induces a high expression of pro collagenase 1 ( pro matrix metalloproteinase 1 ( pro MMP 1 ) ) . ^^^ |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| These findings raise the possibilities that protective levels of AAT in the lungs are insufficient or that elastin degradation in the lungs of these subjects is not dependent upon neutrophil elastase at this time . . ^^^ |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| DESIGN : We examined semiquantitatively the expression of von Willebrand factor , elastin , neutrophil elastase , type 4 collagen , and collagenase in 23 specimens of HCC with spontaneous rupture by immunohistochemistry , and compared them with 30 specimens of HCC without rupture . ^^^ RESULTS : There was a significant decrease of von Willebrand factor , proliferation of degenerated elastin , abnormal distribution of neutrophil elastase , degradation of type 4 collagen , and increase in collagenase production around the blood vessels in ruptured HCC . ^^^ |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| The macrophage elastase enzyme ( MMP 12 ) expressed mainly in alveolar macrophages has been identified in the mouse lung as the main destructive agent associated with cigarette smoking , which gives rise to emphysema , both directly via elastin degradation and indirectly by disturbing the proteinase / antiproteinase balance via inactivation of the alpha 1 proteinase inhibitor ( alpha 1 PI ) , the antagonist of the leukocyte elastase . ^^^ |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| In a patient with a long history of smoking and emphysema , PCP may trigger macrophage activation and an excessive release of leukocyte elastase , leading to elastin destruction in the alveoli . . ^^^ |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| This study compared the degradation of [ ( 14 ) C ] elastin by Pr 3 and its inhibition by alpha ( 1 ) proteinase inhibitor ( alpha ( 1 ) PI ) with the analogous reactions involving two other neutrophil serine proteases , human leukocyte elastase ( HLE ) and cathepsin G ( CatG ) . ^^^ |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| NE can digest elastin , fibrillar and nonfibrillar collagens , and other ECM components in addition to its ability to modify lipoproteins and modulate cytokine and MMP activity . ^^^ |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| Since elastin modulates the mechanical properties of supportive tissues , we examined elastase activity in vaginal tissue from women with pelvic floor dysfunction compared to asymptomatic controls , by comparing overall elastase activity , human neutrophil elastase , cathepsin K , and alpha 1 antitrypsin ( a serine protease inhibitor ) mRNA and protein levels . ^^^ |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| Thus , the effects of pH , NaCl and calcium concentrations on HLE activity , separated from polymorphonculear leucocytes ( PMNLs ) of healthy , young ( < 35 years ) and elderly ( > 75 years ) subjects , were studied by measuring the activity with synthetic substrate and with bovine and human atherosclerotic and non atherosclerotic aortic elastin . ^^^ The elastolytic activity of HLE , of both young and old subjects , increases dramatically on atherosclerotic aortic elastin in the presence of calcium . ^^^ |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| These mice were exposed to 0 . 09J UVB irradiation for 5 months to see if neutrophil elastase was an important factor in elastin remodeling and development of solar elastoses . ^^^ |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| BACKGROUND : Alpha 1 antitrypsin ( AAT ) serves primarily as an inhibitor of the elastin degrading proteases , neutrophil elastase and proteinase 3 . ^^^ |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| Human leukocyte elastase ( HLE ) is a serine protease that very efficiently degrades various tissue matrix proteins such as elastin . ^^^ The imbalance between HLE and its endogenous inhibitors leads to excessive elastin proteolysis and is considered to be responsible for the onset of chronic obstructive pulmonary disease ( COPD ) . ^^^ Human leukocyte elastase ( HLE ) is a serine protease that very efficiently degrades various tissue matrix proteins such as elastin . ^^^ |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| Neutrophil elastase ( NE ) plays an important role in emphysema , a pulmonary disease associated with excessive elastolysis and ineffective repair of interstitial elastin . ^^^ Neutrophil elastase ( NE ) plays an important role in emphysema , a pulmonary disease associated with excessive elastolysis and ineffective repair of interstitial elastin . ^^^ |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| Neutrophil elastase can damage air spaces by degrading elastin , and a variety of extracellular membrane proteins , proteoglycans , and glycoproteins . ^^^ |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| Kinetic analyses were completed using in vitro assays with synthetic and insoluble elastin substrates in the presence of HNE and various heparin preparations ( 14 15 kDa ; 17 19 kDa ) , heparin derived oligosaccharides ( 4 22 saccharides ) , and chemically modified heparins ( 2 O , 6 O , O , and N desulfated ) . ^^^ |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| Nuclear accumulation of activated ERK is associated with transient , peaking at 30 min , induction of c Fos and sustained , observed at 24 48 h , decrease of tropoelastin mRNA levels in NE challenged cells . ^^^ Pretreatment of fibroblasts with AG 1478 or PD 98059 abrogates the NE initiated tropoelastin mRNA suppression . ^^^ We conclude that proteolytically released EGF signals directly via EGFR and ERK to down regulate tropoelastin mRNA in NE challenged lung fibroblasts . . ^^^ |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| It is suggested that these pharmacological effects are accounted for by the combined antioxidative , leukocyte elastase inhibiting and endothelial nitric oxide ( NO ) synthesis enhancing properties of WS 1442 . . ^^^ |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| Escherichia coli and Salmonella recoveries were determined from turkeys sampled by a carcass rinse ( CR ) , a modified rinse with the carcass supported in a swing ( MCR ) , a two site swab of 50 cm 2 at the back and thigh ( 2S ) , a one site swab of 50 cm 2 beneath the wing ( 1S ) , a whole carcass swab of the inner and outer carcass surface ( WS ) , and excision of 25 g of neck skin tissue ( NE ) . ^^^ |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15502 and P08246 |
Pubmed |
SVM Score :0.0 |
| NA |
|