Pubmed abstracts for Protein-Protein Interaction search result :

Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.86561174
Like c Fos and Fra 1 , Fra 2 formed stable heterodimers with c Jun , JunB or JunD in vitro and all these complexes had specific DNA binding activity to AP 1 binding sites ( AP 1 sites ) or related sequences . 0.86561174^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.87467451
Irrespective of enhanced c fos expression , c jun was phosphorylated and became primarily heterodimerized with fra 1 , which was also induced after PDTC incubation . 0.87467451^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :1.4865721
Overall the results suggest that bFGF represses elastin gene transcription by increasing the amount of the Fra 1 that subsequently binds to the 564 to 558 bp as a heterodimer with c Jun to form an inhibitory complex . 1.4865721^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.77653498
From transient transfection analysis , c Jun was found to strongly activate a CYP2J2 luciferase reporter construct , but co transfection with plasmids encoding c Fos or c Fos related antigens , Fra 1 and 2 , abrogated reporter activity . 0.77653498^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.71731877
RESULTS : Transcriptionally active AP 1 in combinations of c Jun , JunD and JunB with Fra 1 , Fra 2 and possibly FosB , are expressed in cardiac myocytes . 0.71731877^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
The fos related antigen 1 ( fra 1 ) gene is closely related to the protooncogene c fos and encodes a component of transcription factor AP 1 . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
Genes strongly expressed at the transcript level as a result of p38 / SAPK2 activation are the transcription factors c jun , fos related antigen 1 ( fra 1 ) , the growth arrest and DNA damage gene gadd 153 and early growth related gene 1 ( egr 1 ) as well as the c srk kinase csk and the nucleotide exchange factor ras GRF . mRNAs significantly down regulated include the insulin receptor IR , the adapter grb 2 , the transcription factor c myc and the defender against apoptotic death , dad 1 . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
One component of the AP 1 complex is the transcription factor Fra 1 ( fos related antigen 1 ) , and we have demonstrated previously that insulin stimulates the expression of Fra 1 mRNA in CHO . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
We report here that c Fos and Fos related antigen 1 ( Fra 1 ) , two inducible components of AP 1 , are recruited to the endogenous interleukin ( IL ) 8 promoter in an IL 1 dependent manner . c Fos activates IL 8 transcription and synergizes in this effect with p 65 NF kappaB . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
Several IE ( immediate early ) genes , including c myc , c jun , egr 1 ( early growth response factor 1 ) , and fra 1 ( fos related antigen 1 ) , displayed PERK dependent expression in MEFs upon disruption of calcium homoeostasis by inhibiting the ER ( endoplasmic reticulum ) transmembrane SERCA ( sarcoplasmic / ER Ca2+ ATPase ) calcium pump . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
ET isopeptides induced a subset of fos and jun mRNAs in mesangial cells , including c fos , fra 1 , c jun , and JunB . fos and jun mRNAs were induced as members of the immediate early gene response . ^^^ Activation of the high affinity ET receptor moderately increased c fos and fra 1 mRNA , whereas activation of the low affinity receptor markedly induced both fos and jun mRNAs . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
In contrast , in differentiation medium , the induction of c fos , c jun , and fos B mRNAs by okadaic acid was transient , whereas fra 1 , jun D , and jun B mRNAs were induced continuously , suggesting that okadaic acid regulates the expression of the c fos and jun family through complex regulatory mechanisms depending on the state of differentiation of the cells . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
LIF has no effect on the mRNA levels for retinoic acid receptor ( RAR ) alpha , RAR beta , RAR gamma , jun A , jun D , c fos , or fra 1 . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
The late induction of c jun and fra 1 mRNA can be prevented by adding the antagonist hirudin 30 min after thrombin , which results in loss of thrombin stimulated increases in c Jun protein , AP 1 DNA binding activity , and AP 1 mediated transactivation . ^^^ These findings suggest that rapid and transient conduction of c fos and c jun mRNA is insufficient to induce prominent changes in gene transcription , while the sustained increase in c jun mRNA and perhaps the late induction of fra 1 mRNA are required for generation of AP 1 DNA binding activity and transactivation through AP 1 . . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
These hematopoietic progenitor lines become quiescent in G0 / G1 after interleukin 3 ( IL 3 ) deprivation , and upon stimulation synchronously enter the cell cycle . 32D cells respond to IL 3 with rapid induction of jun B and c fos , followed by induction of jun D and fra 1 , but no rise in c jun expression . ^^^ FDCP 1 cells show a very different pattern , with induction of c jun , jun D and fra 1 . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
We have shown that while c Fos is the major Fos protein associated with the Jun proteins ( c Jun , JunB , and JunD ) soon after serum stimulation , at later times Fra 1 and Fra 2 are the predominant Fos proteins associated with the different Jun proteins . ^^^ Consequently , Fra 1 and Fra 2 are the main Fos proteins complexed with the Jun proteins in asynchronously growing cells . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
Using Northern blot hybridization and nuclear run on analyses , we examined the modulation of c fos , fos B , fra 1 , c jun , and jun B gene transcripts in Rat 1 fibroblasts after ET treatment . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
Southern analysis indicated that both cell lines contain c jun and fra 1 genes . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
Northern analysis indicates that mRNAs for at least six members of the Fos / Jun family ( c fos , fosB , fra 1 , c jun , junB , and junD ) are expressed in activated Ar 5 cells ; thus the AP 1 sites of the IL 2 promoter may bind different dimeric Fos / Jun complexes at different times after T cell activation , perhaps mediating both positive and negative regulation of the IL 2 promoter . . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
We have compared the binding properties of c JUN , JUN B , and JUN D in the absence or in the presence of c FOS , FOS B , and FRA 1 to different AP 1 and CRE containing oligonucleotides . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
Okadaic acid treatment was found to dramatically increase mRNA transcripts of the jun family of proto oncogenes including c jun , junD , and junB and to a lesser extent the fos family including c fos and fra 1 . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
Insulin binding to its receptor stimulates DNA synthesis and cell division and induces an increase in abundance of mRNA for c fos , c jun , Krox 20 , Krox 24 ( zif / 268 ) , fra 1 , jun B , c myc , and JE . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
Consistent with this result , these cell lines expressed increased levels of mRNAs encoding the AP 1 proteins , c Fos , Fra 1 , c Jun , JunB , and JunD . ^^^ By using the human T cell line ( JPX 9 ) , in which expression of the Tax 1 is inducible , we showed that expression of mRNAs for Fra 1 , c Jun , and JunD was also transactivated by Tax 1 . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
To determine whether these proteins are required for cell cycle progression , we microinjected affinity purified antibodies directed against c Fos , FosB , Fra 1 , c Jun , JunB , and JunD , and antibodies that recognize either the Fos or the Jun family of proteins , into Swiss 3T3 cells and determined their effects in cell cycle progression by monitoring DNA synthesis . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
Analysis of the expression of some growth factor induced early response genes in 5 sis overexpressing cells revealed : ( a ) high and constitutive c myc mRNA levels in SSV NRK cells ; ( b ) unaltered levels of fra 1 , fos B , jun B and krox 20 transcripts ; ( c ) high and constitutive FOS staining due to c FOS and FOS related protein ( s ) ; ( d ) constitutive c JUN and higher JUN D expression . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
Specifically , 50 70 % structural similarity was observed between the basic domain of the DNA binding region of the nuclear proto oncogene products c fos and its related antigen fra 1 , c jun and the cAMP responsive DNA binding protein CREB , with part of the N terminal half region of helix 1b of vimentin . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
Within minutes of addition , agents such as nerve growth factor ( NGF ) , epidermal growth factor ( EGF ) , basic fibroblast growth factor ( bFGF ) and dibutyryl cyclic AMP ( db cAMP ) rapidly activate cellular immediate early genes such as c fos , c jun , jun B , and egr 1 . fra 1 , a member of the immediate early gene family , follows a distinctly later time course of induction than c fos , c jun , jun B , and egr 1 , suggesting that fra 1 may attenuate the action of genes induced earlier . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
Nodularin up regulated induction of c jun , jun B , jun D , c fos , fos B , and fra 1 mRNA transcripts in rat liver after i . p . administration , and the accumulation of the mRNA transcripts was sustained for over 9 h after treatment . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
The AP 1 binding proteins contained c Jun , Jun D , and Fra 1 , but marginal amounts of Jun B and c Fos . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
Fos B , FRA 1 , FRA 2 , Jun B , Jun D , NGFI A ) . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
In addition to Jun B , we found that the T cell AP 1 complex contains the Fra 1 protein , a member of the fos gene family . ^^^ Thus , the stimulation of AP 1 mediated gene expression by IL 1 in EL 4 cells is due to the promotion of Jun B protein accumulation that , in turn , facilitates Jun B heterodimerization with TPA induced Fra 1 protein , thereby forming an active AP 1 complex . . ^^^ Although IL 1 dramatically increases Jun B accumulation , it does not enhance TPA induced Fra 1 protein levels in EL 4 cells . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
Likewise , expression of fra 1 , c jun and junB continued to be high in serum stimulated senescent cells , while induction of fosB was reduced approximately fivefold . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
The clones also expressed high levels of the AP 1 genes c jun and fra 1 . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
In the present study , fra 1 , a close relative of the c fos gene product in terms of its structure and functional activity , behaved like fos in cardiac atriocytes , effecting an approximately 50 % reduction in c jun activatable expression of a human ANP chloramphenicol acetyltransferase ( CAT ) reporter . ^^^ In cardiac ventriculocytes , however , fra 1 effected a synergistic amplification of the c jun response ( a 2 . 5 fold increase over c jun alone ) . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
Our findings demonstrate high levels of c fos , c jun , and jun B mRNA transcripts during the proliferative period of osteoblast development , while expression of fra 1 and fra 2 is enhanced during the differentiation period . jun D is constitutively expressed during the time course exhibiting only a 30 % decline in levels postproliferatively , and fos B mRNA is undetectable by Northern blot analyses . ^^^ At this time , fra 1 expression is completely downregulated , while c fos , fra 2 , c jun , jun B , and jun D show a dramatic enhancement in expression . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
No relationship was observed between the expression of NGF gene and that of c fos , fos B , fra 1 , jun B proto oncogenes . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
Fos related antigen ( Fra 1 ) , junB , and junD activate human involucrin promoter transcription by binding to proximal and distal AP 1 sites to mediate phorbol ester effects on promoter activity . ^^^ We conclude that ( 1 ) two AP 1 sites in the hINV promoter are important elements required for keratinocyte specific expression , ( 2 ) these AP 1 1 sites mediate the phorbol ester dependent increase in promoter activity , and ( 3 ) Fra 1 , junB , and junD may be important regulators of hINV expression in epidermis . . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
BHQ induced expression of c jun , junB , fra 1 , and fra 2 , which encode AP 1 components , but was a poor inducer of c fos and had no effect on fosB . ^^^ Furthermore , Fra 1 repressed AP 1 activity induced by either TPA or expression of c Jun and c Fos . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
Transcriptional activation of the fra 1 gene by AP 1 is mediated by regulatory sequences in the first intron . ^^^ Using the posttranslational FosER induction system , we demonstrate that this AP 1 dependent stimulation of fra 1 expression is rapid , depends on a functional DNA binding domain of FosER , and is a general phenomenon observed in different cell types . ^^^ In vitro mutagenesis and functional analysis of the rat fra 1 gene in stably transfected Rat 1A FosER fibroblasts indicated that basal and AP 1 regulated expression of the fra 1 gene depends on regulatory sequences in the first intron which comprise a consensus AP 1 site and two AP 1 like elements . ^^^ Together , these data identify fra 1 as a unique member of the fos gene family which is under positive control by AP 1 activity . . ^^^ Constitutive expression of c Fos , FosB , Fra 1 , or c Jun in rat fibroblasts leads to up regulation of the immediate early gene fra 1 . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
These elements include a proximal cyclic AMP response element ( CRE ) / AP 1 like motif ( TGACATCA ) that binds c Jun , JunD , CRE binding ( CREB ) , and ATF proteins , a near consensus glucocorticoid response element , and a distal consensus AP 1 site that binds c Fos , Fra 1 , and JunD . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
Antibodies specific for either c Jun or the Fos family ( c Fos , Fos B , Fra 1 , and Fra 2 ) protected NGF deprived neurons from apoptosis , whereas antibodies specific for Jun B , Jun D , or three nonimmune antibody preparations had no protective effect . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
Lithium in combination with other inducers caused delayed increases in both AP 1 binding activity and c jun , c fos and fra 1 gene expression . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
RNA blot hybridization analysis was used to determine steady state messenger RNA levels for c jun , jun B , jun D , c fos , fos B , fra 1 , nup 475 , and zif 268 . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
Although several members of the AP 1 family were able to activate the PLF gene promoter in transient cotransfection experiments , the predominant AP 1 components interacting with the PLF gene promoter in serum stimulated cells were Fra 1 , JunB , and JunD . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
FOS and JUN proteins are induced by ACTH independently of cell cycle stage . c Fos , fos B , fra 1 , fra 2 , c jun , and jun B genes are induced by ACTH , the kinetic profiles for mRNAs and respective protein products being similar , except for a 1 h protein delay . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
Immediate early gene expression was examined by Northern blotting : both thrombin and PDGF induced egr 1 , c fos , c jun , junB , and fra 1 mRNAs within 15 min ; the response was maximal at 30 60 min ( increases ranging from 2 . 9 to 9 . 3 fold over control serum deprived cells ) and returned to base line levels within 2 4 h . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
A single d amphetamine dose ( 7 . 5 mg / kg , i . p . ) enhances behavioral stereotypy and augments brain expression of c fos , fos B , fra 1 , zif 268 , jun B , and c jun by 2 11 fold . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
The cascade includes SRC family kinase members such as lck , fyn , and lyn , activation of Raf 1 and PI 3K , and ras , and increased expression of the fos , fra 1 , and jun protooncogenes . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
The phorbol ester , 12 O tetradeconoylphorbol 13 acetate ( TPA ) , can not support growth of these cells , is a more effective inducer than insulin of c fos , c myc , c jun , jun B , Krox 20 , Krox 24 , fra 1 and JE , and induces fra 1 , JE and c myc with different kinetics from those of insulin . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
Using a seasonal breeder , the European red fox ( Vulpes vulpes ) , as our model , we have examined the expression of five AP 1 family members ( c fos , fra 1 , fra 2 , c jun and junB ) with a view to elucidating their role in the regulation of spermatogenesis . ^^^ Unique patterns of expression , falling into three broad categories , were observed for the five genes : ( 1 ) continuous expression throughout the spermatogenic cycle ( c fos ) ; ( 2 ) expression only at times corresponding to the onset and shutdown of spermatogenesis ( fra 1 , fra 2 and c jun ) ; and ( 3 ) expression only at the onset of the cycle ( junB ) . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
Using in situ hybridization , the expression of c fos , fosB , fra 1 , fra 2 , c jun and junB was studied after 15 min of normothermic and hypothermic ( 33 degrees C ) transient forebrain ischaemia in the rat , induced by common carotid occlusion combined with systemic hypotension . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
Differential expression of the fos and jun family members c fos , fosB , Fra 1 , Fra 2 , c jun , junB and junD during human epidermal keratinocyte differentiation . ^^^ In the present study we describe the localization of c fos , fosB , Fra 1 , Fra 2 , c jun , junB and junD in the normal human epidermis . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
Expression of c Jun and Fra 1 was increased more than threefold in H ras transfectants compared with Balb Neo 1 , 5 myc and H ras / v myc transfectants , but that of c Fos , Jun B and SP 1 was unchanged . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
Furthermore , DNA binding of Jun / Fos proteins , including c Jun and Fra 1 , was decreased under conditions of mitoinhibition , while the level of Fra 1 in nuclear extracts was increased . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
Calcium induced AP 1 DNA binding complexes consist of Fra 1 , Fra 2 , c Jun , JunB and JunD and are independent of c Fos , since the induction of DNA binding activity and the composition of the AP 1 binding complexes are identical in differentiating keratinocytes derived from c fos null and wild type mice . ^^^ In vivo expression patterns suggest that the predominant AP 1 heterodimer in the granular layer consists of Fra 2 and JunB while a JunD and Fra 1 complex predominates the spinous layer of mouse epidermis . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
An oligonucleotide spanning the AP 1 motif at 184 bound , specifically , nuclear factors from RKO cells , and antibodies specific for Jun D , c Jun , or Fra 1 proteins supershifted the complex indicating the presence of these proteins . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
Supershift assays with various AP 1 antibodies identified c Jun , Jun B , and Fra 1 as components of the complex binding to the decorin promoter . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
We observed that : ( a ) c jun mRNA expression is upregulated predominantly by UVB ; ( b ) fra 1 and c myc are downregulated by UVB , whereas both are upregulated by UVA ; ( c ) fra 2 and AP 2 are downregulated modestly by UVB , ( d ) c fos is unaffected , and ( e ) optimal regulation of each gene is achieved at environmentally relevant fluences ( 25 100 J / m2 for UVB and 2500 10 000 J / m2 for UVA ) . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
Fra 1 and Jun B ) . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
Interestingly , the transformation competent c jun and fra 1 oncogenes were induced by 5 erbA in serum free medium and are candidates to mediate 5 erbA effects . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
Overexpression of cDNA derived combinations of the nuclear proteins Jun and Fos or Jun and Fra 1 repressed hARE mediated chloramphenicol acetyltransferase ( CAT ) gene expression in transfected human hepatoblastoma ( Hep G 2 ) cells . ^^^ Further experiments suggested that this repression was due to overexpression of c Fos and Fra 1 , but not due to Jun proteins . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
In the endothelial cells , all Jun and Fos forms ( c Jun , JunB , JunD , c Fos , FosB , Fra 1 and Fra 2 ) were part of the AP 1 complex after PMA induction . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
They also displayed increased AP 1 DNA binding activity which was predominantly composed of cJun and Fra 1 containing dimers . ^^^ Ectopic co expression of cJun and Fra 1 in NIH3T3 fibroblasts led to a transformed phenotype , attenuation of cFos serum inducibility , increased AP 1 activity and Cyclin D 1 accumulation , all characteristics of oncogenic Ras expressing cells . ^^^ These transformed fibroblasts accumulated higher levels of cJun , JunB , Fra 1 and Fra 2 proteins relative to their normal counterparts . ^^^ These results demonstrate that cJun and Fra 1 are crucial mediators of the Ras transformation process . . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
Cross family interaction between the bHLHZip USF and bZip Fra 1 proteins results in down regulation of AP 1 activity . ^^^ Co expression of exogenous Fra 1 restored the AP 1 activity in a dose dependent manner . ^^^ USF specifically interacts with Fra 1 but not with other closely related family members , c Fos , Fra 2 , FosB , or with c Jun . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
To investigate further the involvement of the AP 1 gene family , we examined PTH and PMA stimulation of jun B , jun D , fos B , and fra 1 mRNAs in UMR 106 01 cells . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
FGF 2 up regulates a DNA binding activity that recognizes the MMP 1 AP1 cognate and contains immunoreactive Fra 1 and c Jun . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
The GR AP 1 site bound protein complexes composed of JunD , JunB , Fra 2 , and Fra 1 from L 929 nuclear extracts . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
As for the expression of early response genes , 1 microM nodularin or microcystin LR induced expression of the c jun , jun B , jun D , c fos , fos B and fra 1 genes in the hepatocytes , and the expression of these genes was prolonged up to 24 h , suggesting mRNA stabilization induced by inhibition of protein phosphatases 1 and 2A . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
Analysis of AP 1 protein expression in calcium and TPA treated keratinocytes demonstrated that only TPA increased the expression of c Jun , while Jun B and Jun D were induced by both of these agents . c Fos was expressed only in TPA treated keratinocytes , Fra 2 was expressed only in calcium treated cells , and Fra 1 was expressed in both . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
TNFa increased c Jun protein , but only weakly induced FOS proteins ( c Fos , FosB , Fra 1 , and Fra 2 ) whereas cAMP increased the abundance of several FOS proteins ( c Fos , FosB , Fra 1 , and Fra 2 ) , with little effect on c Jun levels . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
In both cell types , activation of AP 1 DNA binding activity by EGF was accompanied by the recruitment of Fra 1 into AP 1 , detected earlier in 7777 cells than in hepatocytes , and with the transient appearance of c Fos in 7777 cells only . ^^^ In both cell types , c Fos and Fra 1 proteins increased after EGF treatment , but differences in the induction of Jun proteins were noted , with an increase of c Jun in hepatocytes and an increase of JunB in 7777 cells . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
Among them , fra 1 encoding a transcriptional factor activator protein 1 ( AP 1 ) was identified . ^^^ In the present study , overexpression of fra 1 , fra 2 , c jun , junB , and junD mRNAs was demonstrated in RCs by Northern blot analysis . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
We have investigated the effect of a 30 min running bout in untrained subjects on the expression of the mRNAs of all members of the fos and jun gene families , including c fos , fosB , fosBdel , fra 1 , and fra 2 as well as c jun , junB , and junD . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
Supershift analysis demonstrated that JunD was the major moiety contributing to AP 1 DNA binding in unstimulated cells and that c Jun , Fra 1 , and Fra 2 DNA binding were increased whereas JunD DNA binding was decreased in TGF beta 1 stimulated cells . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
Both were activated by the c Fos and c Jun components of AP 1 , but not by Fra 1 . ^^^ In contrast , the K 6 promoter was very strongly activated by all AP 1 proteins , especially by the c Fos + c Jun and Fra 1 + c Jun combinations . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
We found a rapid induction of c fos , c jun and junB at the mRNA level after about 30 min of EGF treatment and a more delayed upregulation of fosB and fra 1 . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
The effect of phorbol 12 myristate 13 acetate ( PMA ) on the regulation of proenkephalin ( proENK ) mRNA level , ENKCRE 2 or AP 1 DNA binding activity , and the mRNA and protein levels of proto oncogenes ( c fos , fra 1 , and c jun ) in primary cultured rat astrocytes were studied . ^^^ The results suggest that newly synthesized AP 1 proteins , such as c Fos , Fra 1 , and c Jun may play important roles in the regulation of PMA induced proENK gene expression in cultured rat astrocytes . ^^^ In addition , PMA caused the induction of c fos , c jun and fra 1 mRNA level and , especially , PMA induced increase of fra 1 mRNA level was further enhanced by CHX treatment at 4 h . ^^^ Furthermore , western immunoblot assay showed that PMA caused induction of c Fos , Fra 1 , and c Jun protein levels . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
The levels of two other immediate early genes , fosb and c jun , also increased transiently after fibroblast contraction , whereas the levels of fra 1 , fra 2 , c myc , and the transcription factor NF kappaB remained the same , indicating that fibroblast contraction caused changes in a selective group of genes . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
These results suggested that induced Fos , FosB , Fra 1 , Jun and JunD proteins constitute the activator protein 1 complex after the administration of haloperidol and sulpiride . ^^^ In contrast , FosB , Fra 1 and JunD appear to constitute the activator protein 1 complex after the administration of SCH 23390 . ^^^ The densities of Fos , FosB , Fra 1 , Jun and JunD immunoreactive nuclei induced by haloperidol and sulpiride in the hippocampus , piriform cortex and accumbens nucleus were higher than those in the control groups . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
The delayed increase in COX 2 mRNA expression was accompanied by the induction of the proto oncogenes c jun , junB , junD , and c fos ( but not FosB or Fra 1 ) . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
Expression of c jun , junB , c fos , fra 1 and fra 2 mRNA in the rat brain following seizure activity and axotomy . ^^^ The patterns of c jun , junB , c fos , fra 1 and fra 2 mRNAs were studied by radioactive and non radioactive in situ hybridization in the adult rat brain following kainate induced seizure activity and axotomy . ^^^ Whereas fra 1 and fra 2 were restricted to the hippocampus , c jun , junB and c fos were additionally induced in the cortex , amygdala and thalamus . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
Blocked shift / supershift analysis indicates that Fos family member proteins especially Fra 1 and Fra 2 are related to progression and no changes found in the Jun family member proteins although they are present in the AP 1 / DNA binding complex . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
FRA 1 expression level modulates regulation of activator protein 1 activity by estradiol in breast cancer cells . ^^^ In conclusion , we demonstrated that ER alpha breast cancer cell lines differ from ER+ cells by a high level of AP 1 DNA binding activity due , at least in part , to high Fra 1 constitutive expression . ^^^ High Fra 1 concentration is crucial for the negative regulation of AP 1 activity by estradiol and thus may take part in estradiol induced inhibition of cell proliferation in ER alpha breast cancer cells transfected with ER alpha expression construct . . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
Fra 1 and c jun were induced by p 53 , resulting in increased AP 1 levels . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
When analysing the composition of the AP 1 complex in more detail and comparing ras induced versus phorbol ester induced changes , we found Fra 1 to be the major component induced in ras transfected but not in phorbol ester treated or non treated parental SCLC cells . ^^^ This finding is paralleled by the observation that among the various members of the Fos and Jun family analysed ( c Fos , FosB , Fra 1 , Fra 2 , c Jun , JunD , JunB ) fra 1 is the only gene to be exclusively expressed in NSCLC cells but not in cells of SCLC origin . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
The concentration of c fos , c jun , c myc , junB , and fra 1 mRNAs was increased in activated peripheral blood lymphocytes incubated with ciprofloxacin compared to that in untreated controls . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
H2O2 also increased the expression of the early immediate genes c jun and fra 1 . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
Insulin stimulated expression of c fos , fra 1 and c jun accompanies the activation of the activator protein 1 ( AP 1 ) transcriptional complex . ^^^ The activator protein 1 ( AP 1 ) transcriptional complex is made up of members of the Fos ( c Fos , FosB , Fra 1 , Fra 2 ) and Jun ( c Jun , JunB , JunD ) families and is stimulated by insulin in several cell types . ^^^ In the current study we show that the AP 1 complex isolated from insulin stimulated cells contained c Fos , Fra 1 , c Jun and JunB . ^^^ The activation of the AP 1 complex by insulin was accompanied by ( 1 ) a transient increase in c fos expression , and the transactivation of the ternary complex factors Elk 1 and Sap1a , in an Erk1 / Erk2 dependent fashion ; ( 2 ) a substantial increase in the expression of Fra 1 protein and mRNA , which was preceded by a transient decrease in its electrophoretic mobility upon SDS / PAGE , indicative of phosphorylation ; and ( 3 ) a sustained increase in c jun expression without increasing c Jun phosphorylation on serines 63 and 73 or activation of the stress activated kinase JNK / SAPK . ^^^ In conclusion , insulin appears to stimulate the activity of the AP 1 complex primarily through a change in the abundance of the components of this complex , although there may be an additional role for Fra 1 phosphorylation . . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
One hour after the intraperitoneal administration of delta 9 THC at a dose of 10 or 15 mg / kg , AP 1 DNA binding activity in the nucleus accumbens increased by 33 and 49 % , respectively , while Western blot showed an increase in both c Fos , FosB , Fra 1 ( Fos related antigen ) and Fra 2 . ^^^ While in the caudate putamen the increase in AP 1 DNA binding was mainly due to an elevation of the c Fos and FosB proteins , the same phenomenon depended on the FosB , Fra 1 and Fra 2 peptides in the cingulate cortex . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
We found that the enhanced level of AP 1 in CSML 100 cells was due to high expression of Fra 1 and Fra 2 proteins , which were undetectable in CSML 0 nuclear extracts . ^^^ Analysis of the transcription of different AP 1 members in various cell lines derived from tumors of epithelial origin revealed a correlation of fra 1 expression with mesenchymal characteristics of carcinoma cells . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
We have examined the expression of members of the AP 1 transcription factor complex in response to stimulation with different doses of LPA . c Fos , c Jun , and JunB are induced rapidly in response to LPA stimulation , whereas Fra 1 and Fra 2 are induced after a significant lag . ^^^ These results suggest that kinetically distinct phases of MAPK activation serve to regulate the expression of distinct AP 1 components such that sustained MAPK activation is required for the induced expression of Fra 1 , Fra 2 , c Jun , and JunB . ^^^ The expression of c Fos is transient , whereas the expression of c Jun , JunB , Fra 1 , and Fra 2 is sustained . ^^^ In contrast , expression of Fra 1 , Fra 2 , and JunB and optimal expression of c Jun are observed only with doses of LPA which induce sustained MAPK activation and DNA synthesis . ^^^ LPA stimulated expression of c Fos , Fra 1 , Fra 2 , c Jun , and JunB is inhibited by the MEK 1 inhibitor PD 098059 , indicating that the Raf MEK MAPK cascade is required for their expression . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
This article reviews findings up to the end of 1997 about the inducible transcription factors ( ITFs ) c Jun , JunB , JunD , c Fos , FosB , Fra 1 , Fra 2 , Krox 20 ( Egr 2 ) and Krox 24 ( NGFI A , Egr 1 , Zif 268 ) ; and the constitutive transcription factors ( CTFs ) CREB , CREM , ATF 2 and SRF as they pertain to gene expression in the mammalian nervous system . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
Supershift analysis identified CREB 1 , JunB , c Fos , Fra 1 , and c Jun in protein complexes isolated from differentiated rabbit tracheal epithelial cells binding to this site . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
There are four genes affected by beta catenin overexpression ; three overexpressed genes code for two components of the AP 1 transcription complex , c jun and fra 1 , and for the urokinase type plasminogen activator receptor ( uPAR ) , whose transcription is activated by AP 1 . ^^^ The direct interaction of the beta catenin T cell factor / lymphoid enhancer factor complex with the promoter region of c jun and fra 1 was shown in a gel shift assay . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
Previously , we have shown that nuclear extracts from cultured mouse keratinocytes induced to differentiate by increasing the levels of extra cellular calcium contain Fra 1 , Fra 2 , Jun B , Jun D and c Jun proteins that bind to the AP 1 DNA binding sequence . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
Supershift analysis of AP 1 / DNA complexes with antibodies against the AP 1 sub units , c Fos , FosB , Fra 1 , Fra 2 , c Jun , JunB , and JunD , revealed that the AP 1 / DNA complexes in the various clones had different compositions . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
Fra 1 potentiates osteoclastic differentiation in osteoclast macrophage precursor cell lines . c Fos , a component of the dimeric transcription factor AP 1 , is necessary for osteoclast formation . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
Gel supershift assays with various specific Fos / Jun antibodies identified Fra 1 , Fra 2 and Jun B binding activity in NSCLC cells to a promoter fragment ( 477 to 438 ) spanning the AP 1 site , whereas SCLC do not appear to express functional Fra or Jun B . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
We show that AP 1 , which is abundant in these cells throughout the whole cell cycle , consists predominantly of Fra 1 / c Jun and Fra1 / JunD heterodimers . ^^^ We conclude that oncogenic ras mediates the activation of the fra 1 gene which results in elevated AP 1 activity throughout the cell cycle . ^^^ Fra 1 containing AP 1 complexes repress the c fos and possibly other immediate early genes thereby preventing the induction of certain delayed early genes such as the T 1 gene in response to mitogenic stimulation . . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
These results suggest a cytoplasmic role for Fra 1 in the neurons , whereas the localization of Fra 1 in the nuclei of reactive astrocytes suggests a participation of this transcription factor in the activation of the AP 1 sequence of selected genes in the early glial response after different brain lesions . . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
Supershift assays revealed that fra 1 participates in the activator protein 1 complex together with c fos , c jun and junB . ^^^ Since fra 1 lacks a transactivation function , we therefore suggest that the retinoid mediated induction of fra 1 might function as a negative regulator of the activator protein 1 activity in human pancreatic carcinoma cells . . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
We focussed our analysis on c Fos ( typical of AP 1 genes which are expressed rapidly and transiently ) and Fra 1 and JunB ( typical of AP 1 genes expressed after a delay but in a sustained manner ) . ^^^ Thrombin stimulated a rapid but transient accumulation of c Fos whereas the expression of Fra 1 , Fra 2 , c Jun and JunB was sustained throughout the G 1 phase of the cell cycle . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
AP 1 stands as a synonym for different proteins such as c Jun , JunB , JunD , c Fos , FosB as well as the Fos related antigens Fra 1 and Fra 2 , which can either homo or heterodimerize to build up a functional transcription complex . ^^^ In nuclear extracts obtained from non tumorigenic cells , Jun family members ( in the order c Jun > JunD > JunB ) were mainly heterodimerized with Fra 1 , a protein , known to be involved in the abrogation of AP 1 activity under certain experimental conditions . ^^^ AP 1 stands as a synonym for different proteins such as c Jun , JunB , JunD , c Fos , FosB as well as the Fos related antigens Fra 1 and Fra 2 , which can either homo or heterodimerize to build up a functional transcription complex . ^^^ Ectopical expression of c fos under a heterologous promoter in ' 444 ' cells induces tumorigenicity and a change of the Jun / Fra 1 ratio towards a constellation initially detected in ' CGL3 ' and HeLa cells . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
Here , we demonstrate , by supershift analysis , that JunB , JunD , Fra 1 , Fra 2 , and c Fos bound to AP 1 but that prior treatment of the cells with TGFbeta reduced dramatically c Fos binding , suggesting that c Fos might be playing a negative regulatory role in clusterin gene expression . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
Constitutive urokinase receptor expression in HCT 116 cells required an intact AP 1 motif in the promoter ( at 184 ) and electrophoretic mobility shifting assays indicated less c Jun , JunD , c Fos and Fra 1 bound to this motif in the K Ras disrupted cells . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
EGCG lowered the level of c jun , whereas TFdiG decreased the level of fra 1 . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
In this study , we have investigated for each factor and regulatory cascade of the intermediate step of immediate early gene induction , that is , c myc , c jun , jun D , jun B , c fos , fos B , fra 1 , fra 2 , and egr 1 ; fra 1 and fra 2 expressions were very low . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
In the present study , the expression of members of the AP 1 family of transcription factors in breast tumors ( n = 53 ) was investigated by Western blot with antibodies specific for each of the AP 1 family members ( c jun , junB , junD and c fos , fosB , fra 1 and fra 2 ) . ^^^ We conclude that strong differences in the expression pattern of AP 1 family members are present in breast tumors , and that certain members of this family , such as fosB and fra 1 , might be involved in the pathogenesis of these tumors . . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
Supershift analysis with antibodies specific for NF kappaB , AP 1 , and NF IL 6 binding proteins showed that the NF kappaB binding protein included p65 / Rel A and p 50 ; AP 1 activity included c jun , junB , junD , and Fra 1 ; and NF IL 6 included C / EBPbeta . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
These results indicate that nicotine treatment may affect the transcriptional activity of many genes through c Fos and c Jun protein expression in neural cells , and that Fra 1 protein may make a contribution . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
Overexpression of Jun causes significant alterations in the composition of AP 1 , decreased junB and increased fra 1 expression and results in an increased biologic aggressiveness . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
Wnt stabilizes cytoplasmic beta catenin , which stimulates the expression of genes including c myc , c jun , fra 1 , and cyclin D 1 . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
Moreover , induction of c fos resulted in the concomitant increase in the expression of fra 1 and c jun , further highlighting the importance of AP 1 transcription factors in chondrocyte differentiation . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
Wnt stabilizes cytoplasmic beta catenin , which stimulates the expression of genes including c myc , c jun , fra 1 , and cyclin D 1 . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
Mice lacking Fos ( encoding c Fos ) develop osteopetrosis due to an early differentiation block in the osteoclast lineage . c Fos is a component of the dimeric transcription factor activator protein 1 ( Ap 1 ) , which is composed mainly of Fos ( c Fos , FosB , Fra 1 and Fra 2 ) and Jun proteins ( c Jun , JunB and JunD ) . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
Using specific antibodies against AP 1 complex proteins , it was demonstrated by gel supershift assay that VT preferentially affected phosphorylated c Jun , Jun B , c Fos , and Fra 2 binding activities , whereas it did not alter Jun D and Fra 1 binding . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
Wnt stabilizes cytoplasmic beta catenin and then beta catenin is translocated into the nucleus where it stimulates the expression of genes including c myc , c jun , fra 1 , and cyclin D 1 . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
The activated AP 1 contained c fos but not c jun , fosB , and Fra 1 . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
The AP 1 complex induced by DCA consisted of JunD , Fra 1 , and c Fos . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
In addition , AA also increased several AP 1 proteins , such as c Fos , Fra 1 , Fra 2 , JunB , JunD , and c Jun , or AP 1 and ENKCRE 2 DNA binding activity . ^^^ However , as well as proto oncoprotein levels , such as Fra 1 , Fra 2 , c Jun , JunB , but not JunD , AA induced increase of proENK mRNA was significantly reduced by the pretreatment with 10 microM of PD 98059 ( 1 . 3 fold ) or 10 microM of SB 203580 ( 1 . 8 fold ) . ^^^ In addition , the activation of both the p 38 and ERK pathways appears to be involved in the AA induced increase of proENK mRNA via activating the expression of proto oncoprotein , such as Fra 1 , Fra 2 , c Jun , and JunB . . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
After cell seeding on an extracellular matrix which allows polarization and expression of the mvdp gene in response to androgens , AP 1 protein accumulation is greatly altered and consists in a loss of JunB , Fra 1 , FosB and a decrease in c Fos , c Jun and Fra 2 , while JunD remained at the same level . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
A possible role of Fra 1 as one of the dimeric proteins generating the AP 1 transcription factor was substantiated by its dose dependent expression in mesothelial cells treated with asbestos in vitro . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
In the first hour of biaxially applied static stretch , the changes in expression of immediate early genes , such as c fos , c jun and fra 1 , were quantified . ^^^ In stretched myocytes , expression of c fos and ANP increased transiently to 227 % and 176 % respectively after 30 min stretch , whereas c jun and fra 1 expression decreased in the 1st hour of stretch . ^^^ In stretched fibroblasts the expression of c fos and fra 1 increased transiently to maxima of 145 % and 146 % respectively after 30 min stretch , whereas c jun expression did not change significantly . ^^^ CM from stretched myocytes reduced c fos and induced c jun expression in myocytes and fibroblasts , reduced fra 1 expression in myocytes but induced fra 1 expression in fibroblasts . ^^^ CM from stretched fibroblasts induced c fos expression and had little effect on c jun expression in myocytes and fibroblasts , induced the fra 1 expression in myocytes but had little effect on fra 1 expression in fibroblasts . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
Immunoelectrophoretic mobility shift ( supershift ) assays identified JunD , Fra 1 , and Fra 2 as the major factors that bind to the AP 1 element . ^^^ Western analysis of the proteins in the nuclear extracts showed that the levels of c Jun , JunB , JunD , FosB , and Fra 2 increased and the levels of c Fos and Fra 1 decreased slightly with calcium treatment . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
The dissection of AP 1 composition in cervical carcinoma cells revealed an inverse relationship between the Fos related antigen Fra 1 and the tumorigenic phenotype . ^^^ Cervical carcinoma cell lines were either negative or expressed only low amounts of Fra 1 ( jointly with c Fos ) within their AP 1 complexes . ^^^ The monitoring of AP 1 composition in electrophoretic mobility super shift assays showed that the amount of Fra 1 was substantially increased within the AP 1 complex of non malignant cells . ^^^ This correlation suggests that the concentration of Fra 1 within the AP 1 transcription complex might be an important marker for predicting the in vivo growth properties of HPV positive cells . . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
The activator protein 1 complex in human keratinocytes consists of dimers of Fra 1 , Fra 2 , c Jun , JunD , and c Fos . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
Determination of factors binding to those sites by Northern analysis and transient transfections identified the requirement of Fra 1 , c Jun , and Cbfa 1 for basal collagenase 3 promoter activity in MDA cells . . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
On the other hand , c fos combined with any of the Jun related proteins failed to stimulate the TIMP 1 promoter , although it was activated by Fra 1 or Fra 2 / Jun related protein heterocomplexes . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
The AP 1 sequence specifically bound to fra 1 , junD , and junB in H 441 lung adenocarcinoma nuclear extracts . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
Therefore , we studied protein expression of 6 cell cycle regulatory proteins ( Rb , p 16 , p 21 , p 27 , cyclin D 1 , and cyclin E ) in protein extracts from 53 breast cancer samples and 4 mammary cell lines and correlated the data with expression of the 7 AP 1 family members ( c jun , junB , junD , c fos , fosB , fra 1 , and fra 2 ) as determined in a previous study . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
Analyses of AP 1 composition revealed that MCP 1 is only expressed in those cells where jun family members were mainly heterodimerized with the fos related protein fra 1 . ^^^ In contrast , in tumorigenic cells the 1 : 1 ratio between jun and fra 1 is disturbed and the MCP 1 gene is no longer expressed . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
In addition , increased amounts and post translational modifications of c Jun , Fra 1 , Fra 2 and ATF 2 proteins account for a high percentage of the increased AP 1 activity . ^^^ High levels of phosphorylated c Jun , Fra 1 , Fra 2 and ATF 2 proteins correlate with malignant phenotypes in the multistage mouse skin carcinogenesis model . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
As a result , Fra 1 and Fra 2 though efficiently form dimers with the Jun proteins , are weak transcriptional activators and inhibit the c Fos dependent activation in transient transfection assay . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
Fra 1 is a c Fos related protein belonging to the AP 1 family of transcription factors . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
The expression levels of protooncogenes ( as c fos , c jun , and fra 1 ) were measured , and as an indication of a hypertrophic response the expression of atrial natriuretic peptide ( ANP ) was measured . ^^^ In stationary cardiomyocytes , CM derived from stretched cardiomyocytes caused decreased c fos and fra 1 expression by 37 and 20 % , respectively ( CM 30 ) , elevated c jun expression by 20 % ( CM 45 CM60 ) , and increased ANP expression by 106 % ( CM 45 ) . ^^^ CM derived from stretched cardiac fibroblasts caused increased c fos expression by 41 % ( CM 60 ) , no significant changes in c jun expression , and increased fra 1 and ANP expression by 39 and 20 % , respectively ( CM 45 ) . ^^^ CM derived from stretched VSMCs induced an initial decrease in c fos expression followed by an increase of 13 % ( CM 45 ) and induced increased c jun , fra 1 , and ANP expression by 39 , 24 , and 22 % , respectively . ^^^ CM 15 CM60 derived from stretched endothelial cells caused decreased c fos , c jun and fra 1 expression by 20 , 25 , and 25 % , respectively , and increased ANP expression by 18 % . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
Supershift analysis suggests that activated AP 1 contained c Jun , JunD , c Fos , FosB , and Fra 1 subunits . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
We examined by immunocytochemistry the localization of the AP 1 family proteins c Jun , JunB , JunD , c Fos , FosB , Fra 1 , and Fra 2 in rat incisor ameloblasts . ^^^ The labeling intensity of the c Jun , JunD , and Fra 2 antibodies was stronger than that of JunB , FosB , and Fra 1 . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
Placental vascularisation requires the AP 1 component fra 1 . ^^^ Fra 1 is an immediate early gene encoding a member of the AP 1 transcription factor family , which has diverse roles in development and oncogenesis . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
H ( 2 ) O ( 2 ) induced egr 1 , fra 1 , and c jun gene expression is mediated by tyrosine kinase in aortic smooth muscle cells . ^^^ In the present study , H ( 2 ) O ( 2 ) induced mitogenic signaling in aortic smooth muscle cells ( ASMC ) was investigated with a specific focus on the roles of tyrosine kinase and tyrosine phosphatase in the regulation of the H ( 2 ) O ( 2 ) stimulated egr 1 , fra 1 , and c jun transcription . ^^^ The results show that H ( 2 ) O ( 2 ) induced increases in egr 1 , fra 1 , and c jun mRNA levels , as measured by Northern blot analysis , are time and dose dependent with the peak of the response within 2 h . ^^^ These data show that H ( 2 ) O ( 2 ) increases egr 1 , fra 1 , and c jun mRNA levels in vascular smooth muscle cells , and the increase in expression of these genes is mediated by activation of tyrosine kinase . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
In 2 DIV cells ( in which glutamate is nontoxic ) , AP 1 comprised combinations of only Fra 1 , Fra 2 , c Jun , and JunD . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
Using a well established model system for studying changes in cellular proliferation induced by Hoxb 4 , we show that AP 1 activity is markedly increased in Hoxb 4 transduced cells due to significant upregulation of Jun B and Fra 1 protein levels . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
Structure function analysis as well as studies with knock out and transgenic mice have assigned distinct functions to c Fos and Fra 1 , two components of the transcription factor AP 1 ( activator protein 1 ) . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
We detected no significant changes in expression levels of AP 1 components c Jun or Fra 1 , adhesion molecules CD 44 or E cadherin , or cell cycle regulator p 53 , which are encoded by genes previously reported to be under the control of AP 1 in some mouse or human cell lines . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
We previously showed that Tax induces the expression of various family members of the transcription factor AP 1 such as c Jun , JunD , c Fos , and Fra 1 at the mRNA level in T cells . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
Transduction of cells with 5 rasHa results in increased AP 1 dependent transcriptional activity , which is also simulated by transfection of keratinocytes with either c Fos or deltaFos B but not Fra 1 , suggesting that the up regulation of c Fos and deltaFos B contributes to this effect . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
While c Fos , Fra 2 and JunB were increased in response to either stimuli , only Fra 1 , c Jun and JunD were increased by PMA . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
Human T cell leukemia virus type 1 ( HTLV 1 ) Tax protein induces the expression of various family members of the transcription factor AP 1 , such as c Jun , JunD , c Fos , and Fra 1 , at the level of RNA expression in T cells . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
One of them was identified as the gene encoding Fra 1 , a component of the AP 1 transcription regulatory complex . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
For the AP 1 factors , Fra 1 expression showed an inverse correlation with PR B expression . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
The 138T allelic variant binds AP 1 complexes consisting primarily of c Jun , JunB and its partners Fra 1 and Fra 2 in rat VSMC . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
HMBA induces c fos and represses cycloheximide induced c jun and fra 1 expression . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
Expression of egr 1 ( krox 24 ) , egr 2 ( krox 20 ) , egr 3 , c jun , junB , c fos , fosB , fra 1 , fra 2 , and CREB genes was analyzed by reverse transcription polymerase chain reaction ( RT PCR ) in Schwann cells isolated from neonatal rat sciatic nerves and in the cell lines MSC 80 ( mouse Schwann cells ) , NIH 3T3 ( mouse fibroblasts ) , and CHO ( Chinese hamster ovary cells ) . ^^^ In contrast , expression of c Jun , Fra 1 , Fra 2 , and CREB mRNA was not affected by T 3 . ^^^ Serum activated all the genes that responded to T 3 and in addition fra 1 and fra 2 , but not c jun and CREB . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
Supershifts with the AP 1 oligonucleotide showed the presence of the Jun ( c Jun , JunB , JunD ) and Fos ( c Fos , FosB , Fra 1 , Fra 2 ) proteins in the untreated and TGF beta treated OA chondrocytes , whereas only Smad proteins ( Smad 2 , 3 , 4 ) were present in the AP 1 binding proteins from the TGF beta treated chondrocytes . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
Previously , we have demonstrated that basic fibroblast growth factor ( bFGF ) decreases elastin gene transcription in confluent rat lung fibroblasts via the binding of a Fra 1 c Jun heterodimer to an activator protein 1 cAMP response element in the distal region of the elastin promoter . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
METHODS : We performed Western blot experiments with specific antibodies for each of the AP 1 proteins ( c jun , junB , junD , c fos , fosB , fra 1 , fra 2 ) with 41 endometrial carcinomas . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
Supershift assays demonstrated that in control cells , AP 1 binding activity was mediated by Jun D / Fra 2 heterodimers , whereas after vinblastine treatment , AP 1 complexes also containing c Jun and Fra 1 were present , suggesting that induction of these latter proteins by vinblastine is functionally significant . ^^^ Specifically , c Jun , expressed at a low level in control cells , was greatly increased and phosphorylated , Jun D was phosphorylated , Jun B underwent phosphorylation and subsequently became undetectable , and Fra 1 expression was also greatly increased . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
According to the study of oncogene and tumor suppressor gene about the origin of thyroid tumor , expression of Fra 1 , one of AP 1 complex , is increased in thyroid neoplasm , though not present in the normal tissue . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
RESULTS : Eighteen genes ( of 1 , 176 ) were found to be up regulated after 24 hours of infection with this obligate intracellular bacterium , among them the glycoprotein 130 family members IL 11 and LIF , the chemokine gene MIP 2 alpha , the transcription factor genes EGR 1 , ETR 101 , FRA 1 , and c jun , the apoptosis related genes IEX 1L and MCL 1 , adhesion molecule genes such as ICAM 1 , and various other functionally important genes . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
Fra 1 , Fra 2 , FosB , JunB , JunD , c Jun , and c Fos levels are increased by EGCG treatment , as is AP 1 factor binding to hINV promoter AP 1 site . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
We investigated by RT PCR whether PROG activated expression of transcription factors : Egr 1 ( Krox 24 ) Egr 2 ( Krox 20 ) , Egr 3 , c jun , jun B , jun D , c Fos , Fos B , Fra 1 , Fra 2 , CREB , ATF 4 , SCIP and Sox 10 in cultured Schwann cells . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
Moreover , we explored the expression of a variety of immediate early genes including NGFI A , fra 1 and c jun , or CREB phosphorylation but found none to be induced by suckling . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
Transactivation of Fra 1 and consequent activation of AP 1 occur extracellular signal regulated kinase dependently . ^^^ Gel shift analysis identified Fra 1 as distinguishing mitogen activated ( P ( + ) ) from nonactivated ( P ( ) ) AP 1 complexes . ^^^ A second AP 1 nonresponsive P ( ) variant that underexpresses Fra 1 gained AP 1 response upon introduction of a Fra 1 expression construct . ^^^ These observations suggest that ERK dependent activation of Fra 1 is required for AP 1 transactivation in JB 6 cells . . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
However , we identified another Fos family member , Fra 1 , together with its transcriptional activation partner , c Jun , as being stably up regulated in GBM cells . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
In unstimulated cells , the 150 to 84 bp region is weakly bound by AP 1 proteins , mainly JunD and Fra 1 . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
We have investigated the expression of the AP 1 transcription factor proteins , fos , fosB , fra 1 , fra 2 , jun , junB , junD , using Western blot analysis , in several types of asynchronously proliferating cells . ^^^ All cells expressed fos , fra 1 and jun proteins and none expressed fosB . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
Utrophin enhancer activity was dependent on an AP 1 site , and in satellite cells of regenerating muscle the AP 1 factors Fra 1 , Fra 2 , and JunD were strongly induced . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
Ribonuclease protection assays demonstrated that increases in mRNA levels of the early response protooncogenes c jun , junB , fra 1 , and fra 2 accompanied cell proliferation at low concentrations of PM whereas apoptotic concentrations of PM caused transient increases in expression of fos and jun family members and dose responsive increases in mRNA levels of receptor interacting protein , Fas associated death domain , and caspase 8 . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
When compared to weakly metastatic C 10 cells , highly metastatic M 4 cells possessed elevated levels of tissue factor cofactor activity , transfected promoter activity , and heterodimeric AP 1 DNA binding complexes containing Fra 1 . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
Treatment with exogenous TGF beta 1 resulted in a failure to induce transcription from an artificial Smad dependent promoter and a failure to down regulate c myc , but resulted in an up regulation of AP 1 associated genes ( Fra 1 , JunB and fibronectin ) . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
Using DNA protein gel shift assays we determined that nerve growth factor stimulates AP 1 binding in both PC 12 and B5P cells , and identified c Fos , FosB , Fra 1 , Fra 2 , c Jun , JunB and JunD in AP 1 complexes . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
Involvement of AP 1 / ARE and Fra 1 . ^^^ Further studies revealed that c Jun and Fra 1 dimers , members of the AP 1 family previously shown to exert a negative effect on phase 2 gene expression , bound to the ARE sequence . ^^^ We propose a novel mechanism of gamma GCSh down regulation by TGF beta 1 that involves the binding of c Jun and Fra 1 dimers to the distal promoter . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
Since these full length fusions have bzip domains , the results suggest that JunB and / or Fra 1 containing dimers may constitute one target of RA for transrepression of AP 1 . . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
A strong correlation existed between UVA induced AP 1 activity and accumulation of c Fos , c Jun and Fra 1 proteins . ^^^ UVA irradiation induced protein expression of c Fos , c Jun , Fra 1 and Fra 2 . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
Fra 1 substitutes for c Fos in AP 1 mediated signal transduction in retinal apoptosis . ^^^ Opposite to wild type mice , Fra 1 , but not c Fos , was detectable in AP 1 complexes of Fos ( Fosl1 / Fosl1 ) mice . ^^^ As Fra 1 lacks transactivation domains , AP 1 may not induce , but rather suppress genes in retinal light damage . . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
Psychosine induced cytokine mediated nuclear translocation of AP 1 and C / EBP by potentiating the expression of Fra 1 and C / EBP delta proteins . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
In contrast , ERK1 / 2 , p 38 ( MAPK ) and src kinases treatment blocked nuclear translocation of almost all the AP 1 members in both models , except Fra 1 , JunD after deformation and Fra 1 , JunB after clinorotation . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
Under these conditions , fra 1 loading to the AP 1 response element was strongly increased . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
When stably expressed in NIH 3T3 cells , c Jun tethered dimer Fra 2 , but not c Jun tethered dimer Fra 1 and c Jun tethered dimer cFos ( the tilde indicates a tethered dimer ) , inhibited G ( 0 ) arrest at confluency and under low serum conditions and specifically activated cyclin A expression . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
We identified de regulated AP 1 , FRA 1 and c myc mediated transcription in cell line BSp 73 ASML . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
Increased Notch 1 signaling , but not Notch 2 , causes a dramatic down modulation of HPV driven transcription of the E6 / E7 viral genes , through suppression of AP 1 activity by up regulation of the Fra 1 family member and decreased c Fos expression . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
A functional activating protein 1 ( AP 1 ) site regulates matrix metalloproteinase 2 ( MMP 2 ) transcription by cardiac cells through interactions with JunB Fra 1 and JunB FosB heterodimers . ^^^ These studies demonstrate that a functional AP 1 site mediates MMP 2 transcription in cardiac cells through the binding of distinctive Fra 1 JunB and FosB JunB heterodimers . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
Data obtained from knockout mice revealed that some components , such as c Fos are key regulators of bone cell differentiation , whereas others , like c Jun , JunB and Fra 1 are essential in embryonic and / or postnatal development . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
In differentiated 3T3 L 1 adipocytes , insulin stimulated the expression of the mRNA for the genes encoding Fra 1 ( > 100 fold ) , which is a component of the AP 1 transcriptional complex , beta actin ( 6 . 0 fold ) and hexokinase 2 ( 2 . 4 fold ) . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
Mesothelial cell transformation requires increased AP 1 binding activity and ERK dependent Fra 1 expression . ^^^ Here , we demonstrate that asbestos induced mesothelial cell transformation is linked to increases in AP 1 DNA binding complexes and the AP 1 component , Fra 1 . ^^^ Elevated levels of AP 1 complexes , including significant increases in c Jun , JunB and Fra 1 , were found in asbestos exposed RPM cells , but only Fra 1 expression was significantly increased and protracted in both asbestos exposed RPM cells and mesothelioma cell lines . ^^^ In summary , we demonstrate that ERK dependent Fra 1 is elevated in AP 1 complexes in response to asbestos fibers and is critical to the transformation of mesothelial cells . . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
Gene disruption studies indicate that the AP 1 family members c jun , junB , and fra 1 are essential for embryonic development , whereas junD , c fos , and fosB are required for normal postnatal growth . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
This effect was specific because palytoxin had little effect on c Jun , JunB , JunD , FosB , Fra 1 , or Fra 2 binding or on overall levels of transcription factor binding . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
Phosphorylated c Jun , JunB , JunD , and Fra 1 , but not c Fos , FosB , or ATF 2 , are detected in the AP 1 DNA binding complex in cells exposed to trivalent arsenicals . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
Immunoblot analyses confirmed that c Jun , JunD , JunB , c Fos , Fra 1 , Fra 2 , and FosB immunoreactive proteins were present in whole cell extracts ( WCE ) of all antral follicles and midluteal phase corpora lutea ( CL ) as well as granulosa cells ( GC ) isolated from different sized antral follicles . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
An electrophoretic mobility shift assay confirmed the AP 1 binding activity in SMCs and showed that the protein bound to the AP 1 site consisted predominantly of c Jun , JunD and Fra 1 . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
MEK 5 is a specific activator of ERK 5 . c Fos and Fra 1 , well known immediate early gene products , are members of the AP 1 family . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
D 3 stimulates activator protein 1 DNA binding activity by a phosphatidylinositol 3 kinase / Ras / MEK / extracellular signal regulated kinase 1 / 2 and c Jun N terminal kinase 1 dependent increase in c Fos , Fra 1 , and c Jun expression in human keratinocytes . 1alpha , 25 Dihydroxyvitamin D 3 added to human keratinocytes increases differentiation through an activation of the transcription factor activator protein 1 . ^^^ Western blotting experiments revealed that 1alpha , 25 dihydroxyvitamin D 3 caused a rapid and transient activation of the mitogen activated protein kinases , extracellular signal regulated kinase 1 / 2 and c Jun N terminal kinase 1 . 1alpha , 25 Dihydroxyvitamin D 3 also enhanced the expression of the activator protein 1 subunits , c Fos , Fra 1 , and c Jun as determined by northern and western blotting . ^^^ Taken together , our results indicate that 1alpha , 25 dihydroxyvitamin D 3 , via binding to the membrane receptor annexin 2 , induces activation of the phos phatidylinositol 3 kinase / Ras / MEK / extracellular signal regulated kinase 1 / 2 and c Jun N terminal kinase 1 signal transduction pathway resulting in increased expression of c Fos , Fra 1 , and c Jun , and subsequently increased activator protein 1 DNA binding activity and gene transcription . . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
The JNK deficient cells exhibited decreased expression of c Jun , JunD , c Fos , Fra 1 , and Fra 2 ; decreased phosphorylation of c Jun and JunD ; and decreased AP 1 DNA binding activity . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
Real time PCR analyses showed peaks of Fra 1 expression at 6 hours after reperfusion , whereas those for c Jun , Jun B , and cyclin D 1 were at 24 hours . ^^^ Fra 1 and Jun B immunoreactivities were found in Mller cells , whereas c Jun and cyclin D 1 immunoreactivities were found in apoptotic retinal neurons . ^^^ Among the immediate early genes , Fra 1 and Jun B immunoreactivities were found in Mller cells whereas c Jun and cyclin D 1 immunoreactivities were found in apoptotic retinal neurons . . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
Deregulated activation of the IL 6 promoter is for the most part mediated by a combined constitutive activation of the nuclear factor ( NF ) kappaB p 50 and p 65 and the activator protein 1 ( AP 1 ) JunD and Fra 1 family members as demonstrated by electrophoretic mobility shift assays , site directed mutagenesis , and transfection experiments . ^^^ Our data demonstrate for the first time that a combined aberrant activation of NF kappaB p 50 and p 65 and AP 1 JunD and Fra 1 in androgen independent prostate cancer cells results in deregulated IL 6 expression , suggesting a novel potential entry point for therapeutic intervention in prostate cancer . . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
By western blot analysis , AP 1 , such as Fra 1 , Jun B , and phospho CREB protein levels were increased by LT and translocation of NFkappaB p 52 from the cytoplasm to the nucleus was increased . ^^^ Electrophoretic mobility shift assay ( EMSA ) showed that AP 1 and NFkappaB DNA binding activities were increased at 6 hr and these AP 1 and NFkappaB DNA bands increased by LT were super shifted when Fra 1 , Jun B , or NFkappaB p 50 antibody was coincubated . ^^^ These findings strongly suggest that , in C 6 cells , Fra 1 , Jun B , NFkappaB p 50 , and NFkappaB p 52 appear to be involved in the regulation of iNOS mRNA induced by LT . . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
The oncogene dependent transcriptional activation involves the cooperation between both Raf dependent and Raf independent pathways and is mediated by an AP 1 site within the fra 1 first intron , which becomes stably occupied by a transcriptionally active Fra 1 containing complex in ras transformed cells . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
Among several immediate early gene products , only Jun B and Fra 1 protein levels were increased by LNT , and ginsenoside Rd attenuated Jun B and Fra 1 protein levels induced by LNT . ^^^ Our results suggest that ginsenoside Rd exerts an inhibitory action against NO production via blocking phosphorylation of ERK , in turn , suppressing immediate early gene products such as Jun B and Fra 1 in C 6 glioma cells . . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
In a Gal 4 fusion assay , Pdcd 4 specifically inhibited activation of c Jun and c Fos activation domains , but did not inhibit activation of JunB , JunD , Fra 1 , or Fra 2 . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
To this end , we studied the kinetics of serum stimulated transcription of c fos , c jun , fra 1 , egr 1 , and cyclinD 1 genes , as well as the effects of sodium butyrate , an inhibitor of histone deacetylase activity , on transcription of these genes in normal REF cells and transformants E1A+ras . . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
Two cell lines with different expression profiles of AP 1 were employed focusing on the Fos family members c Fos , Fra 1 and Fra 2 . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
Here we surveyed human lymphoma samples for expression of JunB and other AP 1 members ( c Jun , c Fos , Fra 1 , JunD ) . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
The AP 1 complex typically comprises two proteins , a Jun ( c Jun , JunB , and JunD ) and a Fos ( c Fos , FosB , Fra 1 , and Fra 2 ) . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
AP 1 subunits c Fos , Fos B , Jun B , and Jun D , but not Fra 1 or Fra 2 , were all induced by CpG ODNs in B cells within 30 minutes of stimulation , followed by c Jun at 1 2 hours . c Jun reached maximum at 6 hours . ^^^ AP 1 subunits c Fos , Fos B , Jun B , and Jun D , but not Fra 1 or Fra 2 , were all induced by CpG ODNs in B cells within 30 minutes of stimulation , followed by c Jun at 1 2 hours . c Jun reached maximum at 6 hours . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
Recent studies indicate a potential role for Fra 1 , a heterodimeric partner of activator protein 1 ( AP 1 ) , in toxicant induced epithelial injury , repair , and cellular transformation . ^^^ Members of the AP 1 family distinctly regulated the fra 1 promoter . ^^^ In particular , coexpression of c Jun , Jun D , and Fra 2 up regulated fra 1 transcription . ^^^ Chromatin immunoprecipitation assays revealed an enhanced recruitment of c Jun , Jun D , and Fra 2 to the endogenous fra 1 promoter upon TPA stimulation . ^^^ These results underscore the regulatory role of c Jun , Jun D , and Fra 2 in TPA inducible fra 1 expression in HBE cells in vivo . . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
Fra 1 targets the AP 1 site / 2G single nucleotide polymorphism ( ETS site ) in the MMP 1 promoter . ^^^ In this study we demonstrate that the expression of Fos like region antigen ( Fra 1 ) , an AP 1 transcription factor component that also correlates strongly with neoplastic disease , is necessary for MMP 1 transcription in A 2058 melanoma cells . ^^^ This study provides evidence that , in cooperation with the 2G DNA polymorphism , the AP 1 family member , Fra 1 , contributes to the high constitutive expression of MMP 1 in melanoma cells . . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
DEP induced fra 1 expression correlates with a distinct activation of AP 1 dependent gene transcription in the lung . ^^^ Overexpression of Fra 1 downregulated c Jun and Nrf 2 enhanced AP 1 and ARE mediated reporter gene expression , respectively . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
Activator protein 1 ( AP 1 ) binding to DNA increased more in VSMC from old versus young rats ( P < 0 . 02 ) and was related to increased expression of its components , c Fos , Fra 1 , and JunD . ^^^ Activator protein 1 ( AP 1 ) binding to DNA increased more in VSMC from old versus young rats ( P < 0 . 02 ) and was related to increased expression of its components , c Fos , Fra 1 , and JunD . ^^^ PD 98059 , a MEK inhibitor , attenuated AP 1 activation , lowered c Fos and Fra 1 protein levels and reduced cell number and cells positive for proliferating cell nuclear antigen in old . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
Overexpression of fra 1 leading to proliferation inhibition of C 6 glioma cells is consistent with the concept that fra 1 functions as a negative regulator of AP 1 activity . . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
In cells containing oncogenic Ras , the major components of AP 1 are Fra 1 and c Jun . ^^^ We show that c JUN and FRA 1 expression is dependent on ERK activity and that different thresholds of ERK activity control the expression of FRA 1 . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
In w 3211 , c Jun , JunD and Fra 1 were involved in AP 1 binding , while in w 1205 , overall AP 1 binding activity was decreased significantly and supershift binding was detected only with JunD antibodies . ^^^ In metastatic c 81 46A and A 375 cells , only JunD was involved in AP 1 binding activity , but in a third ( c 83 2c ) c Jun , JunD and Fra 1 were present . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
The use of a dominant negative ERK 5 construct caused selective downregulation of c jun expression , whereas inhibition of Src by PP 2 or MEK 1 by PD 98059 caused decreases in c fos , fra 1 and c jun expression in asbestos exposed C 10 cells . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
FGF 2 receptor signaling requires MEKK 2 for induction of mRNA for c Jun , Fra 1 , and Fra 2 , components of the AP 1 transcription complex . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
Static stretch induced an increase in the expression of c fos , fosB , fra 1 , c jun , and junB . ^^^ The peak induction for fosB and c jun occurred at 1 h , whereas the peak of fra 1 induction occurred between 1 and 2 h after application of stretch . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
On the contrary , restoration of Fra 1 , Fra 2 , c Jun , p 50 , p 65 , C / EBPalpha and C / EBPbeta expression levels in MCF 7 cells led to a promoter activity comparable to that observed in MDA MB 231 cells . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
In MES 13 cells , the AP 1 motif is bound by Fra 1 , Fra 2 , Jun B and Jun D . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
To determine whether NF kappaB activity is involved in the regulation of fos expression in response to various stimuli , we analyzed activity of AP 1 and expression of fos , fosB , fra 1 , fra 2 , jun , junB , and junD , as well as AP 1 downstream target gene VEGF , using MDAPanc 28 and MDAPanc 28 / IkappaBalphaM pancreatic tumor cells and wild type , IKK 1 / , and IKK 2 / murine embryonic fibroblast cells . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
The role of the AP 1 transcription factors c Fos , FosB , Fra 1 and Fra 2 in the invasion process of mammary carcinomas . ^^^ Members of the Fos family of AP 1 transcription factors ( c Fos , FosB , FosB 2 , Fra 1 and Fra 2 ) are able to form dimers with Jun proteins which bind to the regulatory sequences of target genes . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
Moreover , supershift analysis identified that JunB and Fra 1 are major components involved in the PG mediated induction of AP 1 DNA binding . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
Furthermore , experiments revealed that the protein and mRNA expression of the AP 1 subunits c Fos , Fra 1 and c Jun was reduced in lesional psoriatic skin compared with nonlesional psoriatic skin , whereas the protein and mRNA expression of the subunit JunB was increased . ^^^ Topical application of the vitamin D analogue calcipotriol under occlusion to involved psoriatic skin for 4 days resulted in an increase in AP 1 DNA binding activity , and an increase in the protein and mRNA expression of c Fos , Fra 1 and c Jun , together with a decrease in JunB protein and mRNA expression . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
In situ hybridization was used to confirm the localization of 8 of these genes to astrocytes : these included GDNF , NGF , bFGF , TNF alpha , MIP 1alpha , c jun , Fra 1 and Fra 2 . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
Gel shift experiments with nuclear extracts of hypertrophic chondrocytes revealed FosB and Fra 1 as candidates regulating AP 1 factors binding to the TRE site . ^^^ Quantitative analysis of AP 1 factor mRNA levels in distinct fractions of fetal bovine epiphyseal chondrocytes by real time PCR confirmed significant levels of FosB and Fra 1 mRNA besides other AP 1 factors in hypertrophic chondrocytes . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
Resveratrol reduced both activator protein 1 ( AP 1 ) DNA binding and transcriptional activities , and supershift assay revealed that AP 1 composition was shifted from c Jun / JunD / Fra 1 to JunD / Fra 1 / Fra 2 , with markedly increased JunD , Fra 1 , and Fra 2 protein expression levels in the nucleus . ^^^ Although H ( 2 ) O ( 2 ) restored participation of c Jun in AP 1 complexes , H ( 2 ) O ( 2 ) addition did not affect the induction of Fra 1 and Fra 2 by resveratrol nor the morphological changes . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
In our study , we investigated the AP 1 binding activity and the expression pattern of different members of the AP 1 transcription factor family ( c Jun , JunB , JunD , c Fos , FosB , Fra 1 and Fra 2 ) in different grades of cervical lesions starting from mild dysplasia to invasive cervical tumors , including normal control tissues , using specific antibodies raised against each of the AP 1 members . ^^^ Results indicate that though AP 1 showed high binding activity and the majority of its members were highly expressed in tumor tissues , there is a distinct pattern of gradual increase of c fos and a concomitant decrease of fra 1 expression that perfectly match the progression of cervical lesions . ^^^ Both in vivo and in vitro studies demonstrate that the overexpression of c fos and downregulation of fra 1 expression as well as a change in the dimerization pattern of the AP 1 complex seem to play a crucial role during progression to malignancy . ^^^ Observation of curcumin mediated complete downregulation of AP 1 binding activity and reversal of c fos / fra 1 transcription to a normal state in tumorigenic HeLa cells represents a novel mechanism that can control transcription of pathogenic HPVs during keratinocyte differentiation and progression of cervical cancer . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
We also show that asbestos induced mRNA levels of fos / jun proto oncogenes , fra 1 transactivation , and AP 1 to DNA binding activity are glutathione dependent . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
Exposure to CS caused a marked upregulation of c Jun , c Fos , and Fra 1 , but not of Fra 2 , Jun B , and Jun D expression . ^^^ Similarly , the specific inhibitors of extracellular signal regulated kinase ( ERK ) , c Jun NH 2 terminal kinase ( JNK ) , and p 38 kinase signaling markedly suppressed fra 1 induction . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
Malignant transformation is enhanced by near elimination of p 21 and PC 3 , induction of AP 1 ( upregulation of JUN B , c JUN , FRA 1 ) , activation of the NF kB pro survival pathway , and inhibition of the TGF beta pro apoptotic pathway possibly in response to changes in the expression of nerve growth factor ( NGF ) 1 A and NGFI B . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
HTLV 1 encoded Tax elevates AP 1 activity through the induction of AP 1 family member gene expression , including c Jun , JunD , c Fos , and Fra 1 . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
Previously , we showed that the mRNA coding for Fra 1 , a FOS family member and an AP 1 transcription factor component , was highly expressed in the more invasive estrogen receptor negative ( ER ) breast cancer cell lines . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
Notably , Ras mediated transformation is characterized by the upregulation of particular AP 1 members , such as c Jun and Fra 1 . ^^^ The ARF promoter is regulated specifically by AP 1 heterodimers containing Fra 1 . ^^^ Overexpression of c Jun approximately Fra 1 dimers in primary murine fibroblast cells led to the upregulation of the endogenous ARF protein and growth arrest . ^^^ Conversely , inhibition of c Jun or Fra 1 protein levels resulted in decreased ARF expression . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
AP 1 DNA binding activity consists primarily of JunD , c Fos , and Fra 1 , and is accompanied with the increased expression and phosphorylation of these subunits . ^^^ While inhibition of JNK mainly prevents expression and phosphorylation of JunD and c Jun , inhibition of the ERK pathway suppresses both phosphorylation and expression of Jun proteins , and expression of c Fos and Fra 1 . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
Here , we found that MX 1 , a human breast cancer cell line lacking estrogen receptors , exhibited higher AP 1 activity and expressed higher levels of c Jun , c Fos , and Fra 1 when compared with conventional estrogen receptor positive human breast cancer cell lines . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
This uncoupling required the maintenance of high levels of the activator protein 1 family member , Fra 1 , via a mitogen activated protein / extracellular signal regulated kinase dependent pathway . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
RXM at more than 2 . 5 microg / ml also suppressed AP 1 ( Fra 1 and Jun B ) activation in 4 h cultured cells . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
Furthermore , increased DNA binding activity of the AP 1 transcription factor family members FRA 1 and c Jun was observed . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
A gel mobility supershift analysis revealed interaction of the AP 1 factors , Fra 1 and JunB , with this element . ^^^ Treatment with 12 O tetradecanoylphorbol 13 acetate ( TPA ) , a protein kinase C activator , increased hINV gene expression , a response that correlates with increased AP 1 factor ( Fra 1 and JunB ) binding to the hINV gene AP 1 5 response element . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
Using ChIP assays we show that , in contrast to c Jun , SRF , Elk 1 , ATF 1 and CREB proteins bind to SRE and ATF sites of the FRA 1 promoter , constitutively . ^^^ RNAi mediated knockdown of endogenous SRF , ELK 1 and c JUN protein expression significantly reduced TPA stimulated FRA 1 promoter activity . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
In addition , G 120 increased the expression of c Jun , Fra 1 , and Fra 2 , but did not affect the expression of c Fos . ^^^ Collectively , it is believed that G 120 exerts an important role in the induction of apoptosis , suppression of NF kappaB activation , and induction of c Jun / Fra 1 or c Jun / Fra 2 dimerization in MCF 7 cells . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
The AP 1 family member most specifically affected by ascochlorin was Fra 1 . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
In addition , the expression of c jun and fra 1 , which are components of the transcription factor AP 1 , was elevated in 12 / 15 LOX transfected cells , in which cytokine dependent binding of AP 1 to the sPLA 2 IIA promoter was increased significantly . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
Nuclear and cytoplasmic expression levels of c Jun and Fra 1 proteins were markedly elevated in adenomas , adenocarcinomas and neuroendocrine carcinomas compared with nonneoplastic colorectal epithelial cells . ^^^ Up regulation of c Jun and Fra 1 is an early event in human colorectal tumorigenesis . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
A comparison of the microarray and proteomic datasets allowed for the identification of a number of candidate proteins that are post transcriptionally regulated by elevated inorganic phosphate , including Fra 1 , a member of the activator protein 1 family of transcription factors . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
In this study , we analyzed the mRNA expression levels of nine Wnt genes , including c JUN , c MYC , CYCLIN D 1 , FRA 1 , NKD 1 , ITF 2 , MMP 7 , uPAR , and beta TRCP , by competitive reverse transcription PCR . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
For this reason in this study , we investigated the expression pattern of the AP 1 family in GTDs and compared it with the expression in normal placenta using immunohistochemistry with specific polyclonal antibodies against all members of the AP 1 family ( JunB , JunD , c Jun , c Fos , FosB , Fra 1 , Fra 2 ) . ^^^ In choriocarcinomas , there was a strong expression for c Jun , JunD , Fra 1 , and Fra 2 . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
Levels of other AP 1 and NF kappaB family members were either unaffected ( i . e . , JunB ) or increased ( i . e . , Fra 1 ) . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
Overexpression of Bcl 2 induced the nuclear transcription factor activator protein 1 family , including the c Jun , JunD , c Fos , FosB , and Fra 1 proteins . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
Recent evidences have indicated that Nrf 2 protein , which binds to the erythroid transcription factor ( NF E 2 ) binding sites , and its interaction with other oncoproteins such as c Jun , Jun D , Fra 1 and Maf play a key role in the regulation of GCL . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
The mRNA expressions of C / EBPbeta , C / EBPdelta , C / EBPgamma , c Jun , JunB , c Fos , Fra 1 and IkappaBalpha and protein level of IkappaBalpha were all unaffected by VIP . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
Immunodepletion gel shift assays demonstrated that GP 5 EpRE bound JunB , c Jun , FosB , and Fra 2 from unstimulated cells , and that after exposure to HNE , EpRE binding complexes contained nuclear factor erythroid 2 related factor ( Nrf ) 1 , Nrf 2 , JunB , c Jun , FosB , c Fos , Fra 1 , and Fra 2 . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
Members of the Fos family ( c Fos , FosB and its smaller splice variants , Fra 1 and Fra 2 ) dimerise with Jun proteins to form the AP 1 transcription factor complex . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
Short term expression of Ec1WVM up regulated two AP 1 family members , c jun and fra 1 , but was insufficient to induce complete mesenchymal transition . ^^^ Using a dominant negative mutant of c Jun ( TAM 67 ) and RNA interference mediated silencing of c Jun and Fra 1 , we demonstrated that AP 1 was required for cell motility stimulated by the expression of Ec1WVM . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
Electrophoretic mobility shift assays , co immunoprecipitation , and chromatin immunoprecipitation analyses suggested an association between galectin 3 , c Jun , and Fra 1 in forming a complex at the AP 1 site on the MUC 2 promoter . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
Subgroup analysis showed tumor specific binding for c Fos in 58 % , for c Jun in 50 % , for JunD in 39 % , and for Fra 1 in 4 % of cases . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
Converging results consolidate activator protein 1 ( AP 1 ) transcription factor as the pivotal downstream effector in the early response of stress sensitive cells to mechanical loading , and the Fra 1 , Fra 2 , JunB and JunD members of the AP 1 transcription factor family , as mediators in bone remodelling and apoptotic phenomena . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
Embryonic lethality of various AP 1 knock outs , e . g . for Jun , JunB , Fra 1 and Fra 2 largely prevented functional studies in vivo . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
Wnt stabilizes cytoplasmic beta catenin and then the accumulated beta catenin is translocated into the nucleus , where it activates the transcriptional factor T cell factor ( Tcf ) / lymphoid enhancer factor ( Lef ) , and thereby stimulates the expression of genes including c myc , c jun , fra 1 , and cyclin D 1 . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
Gel shift , enzyme linked immunosorbent , and chromatin immunoprecipitation assays revealed a strong induction of IL 18 mediated AP 1 ( c Fos , c Jun , and Fra 1 ) and NF kappaB ( p 50 and p 65 ) activation and stimulation of MMP 9 promoter dependent reporter gene activity in an AP 1 and NF kappaB dependent manner . ^^^ Ectopic expression of p 65 , c Fos , c Jun , and Fra 1 induced MMP 9 promoter activity . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
INTRODUCTION : Tg mice overexpressing Fra 1 , a component of the transcription factor activator protein 1 ( AP 1 ) , show progressive osteosclerosis caused by cell autonomous abnormalities in osteoblasts . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
In HL 60 cells in an early stage of differentiation , the principal AP 1 components detected by gel shift analysis include c jun , ATF 2 , fos B , fra 1 , and fra 2 . ^^^ Following the addition of 1 nmol / L of 1 , 25D , the cellular content of each of these four proteins markedly increased in a sustained manner , whereas the increases in c fos , fra 1 , fra 2 , and jun D were minimal , if any . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
We further demonstrated that Tam 67 suppressed the expression of AP 1 dependent genes ( TIMP 1 , vimentin , Fra 1 , and fibronectin ) and the AP 1 dependent growth regulatory genes ( cyclin D 1 and c myc ) in AP 1 blocked mammary glands . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
For this reason , nine homologues of the AP 1 leucine zipper region have been characterized : Fos ( c Fos , FosB , Fra 1 , and Fra 2 ) , Jun ( c Jun , JunB , and JunD ) , and semirational library designed winning peptides FosW and JunW . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
Fra 1 is a member of the activator protein 1 ( AP 1 ) family of transcription factors and , due to the lack of transactivation domain Fra 1 , can suppress activation of AP 1 . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
Antisense constructs targeting the AP 1 subunits Fra 1 and Fra 2 had no inhibitory effect on pneumococci induced IL 8 release . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
Supershift assays identify that the bound proteins at this region are c Jun and Fra 1 . ^^^ Real time PCR analysis showed more than 4 fold increase in the binding of c Jun and a 1 . 6 fold increase in the binding of Fra 1 in this region and this up regulation corresponds to an increased binding of acetylated histone H 4 in this region . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
MATERIAL AND METHODS : From cDNA microarray screening of those specimens , several gene clusters , including Nos 2 and the transcription factors Fos , Fosl 1 , Jun and Nfkb 1 , were identified as possible upstream regulators of Na+ transport protein expression . ^^^ Gene expression for Fos was suppressed at both times , while that for Fosl 1 and Jun was augmented at 12 h and suppressed at 48 h . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
The over expression of fosl 1 could be related to the event in the induction process leading to the expression of GST pi , DT diaphorase , uPAR and PAI 1 through AP 1 . ^^^
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
NA
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
NA
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
NA
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
NA
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
NA
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
NA
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
NA
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
NA
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
NA
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
NA
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
NA
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
NA
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
NA
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
NA
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
NA
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
NA
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
NA
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
NA
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
NA
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
NA
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
NA
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
NA
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
NA
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
NA
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
NA
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
NA
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
NA
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
NA
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
NA
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
NA
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
NA
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
NA
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
NA
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
NA
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
NA
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
NA
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
NA
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
NA
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
NA
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
NA
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
NA
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
NA
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
NA
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
NA
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
NA
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
NA
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
NA
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
NA
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
NA
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
NA
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
NA
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
NA
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
NA
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
NA
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
NA
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
NA
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
NA
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
NA
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
NA
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
NA
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
NA
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
NA
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
NA
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
NA
Interacting proteins: P15407 and P05412	Pubmed	SVM Score :0.0
NA