| Interacting proteins: P14653 and P55347 |
Pubmed |
SVM Score :0.0 |
| In PREP 1 overexpressing cells , HOXb 1 induction was more sustained ( 3 days versus 1 day ) and the induced level of MEIS 1b , another TALE ( three amino acid loop extension ) protein involved in embryonal development , was higher . ^^^ Thus an increase in PREP 1 leads to changes in the fate determining HOXb 1 and has therefore important functional consequences . . ^^^ |
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| Interacting proteins: P14653 and P55347 |
Pubmed |
SVM Score :0.0 |
| Human PREP 1 , a novel homeodomain protein of the TALE super family , forms a stable DNA binding complex with PBX proteins in solution , a ternary complex with PBX and HOXB 1 on DNA , and is able to act as a co activator in the transcription of PBX HOXB 1 activated promoters ( Berthelsen , J . , Zappavigna , V . , Ferretti , E . , Mavilio , F . , Blasi , F . , 1998b . ^^^ PREP 1 is able to super activate the PBX HOXB 1 autoregulated Hoxb 1 promoter , and we show that all three proteins , PREP 1 , PBX and HOXB 1 , are present together in the mouse rhombomere 4 domain in vivo , compatible with a role of PREP 1 as a regulator of PBX and HOXB 1 proteins activity during development . . ^^^ |
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| Interacting proteins: P14653 and P55347 |
Pubmed |
SVM Score :0.0 |
| DNA binding assays and cell transfection experiments revealed that the bipartite core sequence of FPB ( 5 ' ATCAATCA 3 ' ) binds the PBX1 / 2 , PREP 1 , and HOXB 1 proteins , and this in turn leads to promoter transactivation . ^^^ In the presence of all three nuclear factors , cooperative interactions between recombinant PBX 1 and PREP 1 or PBX 1 and HOXB 1 result in binding of the heterodimers to FPB in vitro . ^^^ Similarly , overexpression of different combinations of PBX 1 , PREP 1 , and HOXB 1 transactivates FPB driven transcription . ^^^ |
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| Interacting proteins: P14653 and P55347 |
Pubmed |
SVM Score :0.0 |
| Surprisingly , GST pull down assays revealed that the homeodomains of such diverse proteins as Chx 10 , Six 3 , Lhx 2 , En 1 , Prep 1 , Prox 1 , and HoxB 1 could all bind to Pax 6 , and several of these enhanced Pax 6 mediated transactivation upon coexpression . ^^^ |
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| Interacting proteins: P14653 and P55347 |
Pubmed |
SVM Score :0.0 |
| Like PREP 1 , PREP 2 PBX heterodimers are capable of forming ternary complexes with HOXB 1 . ^^^ |
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| Interacting proteins: P14653 and P55347 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P14653 and P55347 |
Pubmed |
SVM Score :0.0 |
| NA |
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