| Interacting proteins: P14653 and P40424 |
Pubmed |
SVM Score :0.68738166 |
| In the presence of all three nuclear factors , cooperative interactions between recombinant PBX 1 and PREP 1 or PBX 1 and HOXB 1 result in binding of the heterodimers to FPB in vitro . 0.68738166^^^ |
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| Interacting proteins: P14653 and P40424 |
Pubmed |
SVM Score :0.0 |
| Binding of Hoxb 1 to these elements in vitro requires cofactors , and the motifs closely resemble the consensus binding site for pbx 1 , a homolog of the Drosophila extradenticle ( exd ) homoedomain protein . ^^^ |
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| Interacting proteins: P14653 and P40424 |
Pubmed |
SVM Score :0.0 |
| Heterodimer contacts are mediated by the hexapeptide of HoxB 1 , which binds in a pocket in the Pbx 1 protein formed in part by a three amino acid insertion in the Pbx 1 homeodomain . ^^^ The Pbx 1 DNA binding domain is larger than the canonical homeodomain , containing an additional alpha helix that appears to contribute to binding of the HoxB 1 hexapeptide and to stable binding of Pbx 1 to DNA . ^^^ |
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| Interacting proteins: P14653 and P40424 |
Pubmed |
SVM Score :0.0 |
| NMR studies of the pbx 1 TALE homeodomain protein free in solution and bound to DNA : proposal for a mechanism of HoxB 1 Pbx1 DNA complex assembly . ^^^ The crystal structure of a human HoxB 1 Pbx1 DNA ternary complex revealed that interactions between the two proteins are mediated by the HoxB 1 hexapeptide , which inserts into a hydrophobic pocket in Pbx 1 . ^^^ In contrast , no change in conformation is observed upon mixing the HoxB 1 protein with Pbx 1 in the absence of DNA . ^^^ |
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| Interacting proteins: P14653 and P40424 |
Pubmed |
SVM Score :0.0 |
| Despite the presence of HOXB 1 and PBX 1 , this enhancer fails to activate reporter gene expression in retinoic acid treated P 19 cell monolayers . ^^^ |
|
| Interacting proteins: P14653 and P40424 |
Pubmed |
SVM Score :0.0 |
| We previously showed that HOXB 1 and PBX 1 activate transcription from the b 1 ARE by binding to sequences required for the expression of a reporter gene in rhombomere 4 in vivo . ^^^ |
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| Interacting proteins: P14653 and P40424 |
Pubmed |
SVM Score :0.0 |
| Herein we describe NMR structural calculations of the hexapeptide of HoxB 1 ( Nalpha acetyl Thr Phe Asp Trp Met Lys amide ) that has been shown to mediate binding between HoxB 1 and Pbx 1 and a hexapeptide consensus sequence ( Nalpha acetyl Leu Phe Pro Trp Met Arg amide ) . ^^^ This observation implies that this region is at least partially ' preformed ' and thus ready to interact with Pbx 1 and stabilize binding of Pbx 1 and HoxB 1 to DNA . ^^^ |
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| Interacting proteins: P14653 and P40424 |
Pubmed |
SVM Score :0.0 |
| We show that , in P 19 embryonal carcinoma cells , GCs potentiate RA induced expression of the murine Hoxb 1 gene through an autoregulatory element , b 1 ARE , recognized by the Pbx 1 and HOXB 1 homoeodomain proteins . ^^^ |
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| Interacting proteins: P14653 and P40424 |
Pubmed |
SVM Score :0.0 |
| We report that the human Pbx 1 and HOXB 1 proteins can cooperatively activate transcription through a genetically characterized Hox target , i . e . an autoregulatory element directing spatially restricted expression of the murine Hoxb 1 gene ( b 1 ARE ) in the developing hindbrain . ^^^ On the b 1 ARE , only a restricted subset of HOX proteins ( HOXA 1 , HOXB 1 , HOXA 2 ) are able to bind cooperatively with Pbx 1 and activate transcription . ^^^ The DNA binding and protein protein interaction functions of HOXB 1 and Pbx 1 are all necessary for the assembly of a transcriptionally active complex on the b 1 ARE . ^^^ Functional dissection of the complex allowed the localization of the main activation domain in the HOXB 1 N terminal region , and of an additional one in the C terminal region of Pbx 1 contained in the Pbx1a but not in the alternatively spliced Pbx1b isoform . ^^^ |
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| Interacting proteins: P14653 and P40424 |
Pubmed |
SVM Score :0.0 |
| Co expression of either HOXA 1 or HOXB 1 with Pbx 1 transactivated EphA 2 enhancer dependent reporter gene expression . ^^^ |
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| Interacting proteins: P14653 and P40424 |
Pubmed |
SVM Score :0.0 |
| These phenotypes are similar to those previously reported in Hoxa 3 ( / ) single mutants and Hoxa 1 ( / ) ; Hoxb 1 ( / ) or Hoxa 3 ( + / ) ; Hoxb 3 ( / ) ; Hoxd 3 ( / ) compound mutants , suggesting that Pbx 1 acts together with multiple Hox proteins in the development of the caudal pharyngeal region . ^^^ |
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| Interacting proteins: P14653 and P40424 |
Pubmed |
SVM Score :0.0 |
| To examine the contribution of MEIS proteins to this response , we used the chromatin immunoprecipitation assay to show that MEIS 1 in addition to PBX 1 , HOXA 1 , and HOXB 1 was recruited to a known PBX . ^^^ |
|
| Interacting proteins: P14653 and P40424 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P14653 and P40424 |
Pubmed |
SVM Score :0.0 |
| NA |
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