| Interacting proteins: P01266 and P14625 |
Pubmed |
SVM Score :0.65590032 |
| This was accompanied by a major increase in Tg directly associated with GRP 94 and an increase in the ER pool size of Tg . 0.65590032^^^ |
|
| Interacting proteins: P01266 and P14625 |
Pubmed |
SVM Score :0.0 |
| The IRE1p expressing transfectants exhibited a modest ( 2 fold ) enhancement of both the basal and Tg induced level of grp 78 and grp 94 , two coordinately regulated grp genes . ^^^ |
|
| Interacting proteins: P01266 and P14625 |
Pubmed |
SVM Score :0.0 |
| By coimmunoprecipitation in rat thyroid cells , we were able to demonstrate that BiP , grp 94 , ERp 72 , and grp 170 , four proteins believed to function as specific molecular chaperones , complex with Tg during its maturation . ^^^ BiP , grp 94 , and ERp 72 were also found to associate with Tg in cells in which misfolding was induced by perturbing ER calcium stores . ^^^ |
|
| Interacting proteins: P01266 and P14625 |
Pubmed |
SVM Score :0.0 |
| An endoplasmic reticulum chaperone of the hsp 90 family , GRP 94 , was also associated in Tg chaperone complexes . ^^^ |
|
| Interacting proteins: P01266 and P14625 |
Pubmed |
SVM Score :0.0 |
| Regarding the colloidal insoluble multimerized Tg ( m Tg ) , which bears dityrosine bridges and is present in the follicular lumen , a mild oxidative system generated different soluble forms of Tg , more or less compacted by hydrophobic associations , and linked with Grp 78 and Grp 94 . ^^^ |
|
| Interacting proteins: P01266 and P14625 |
Pubmed |
SVM Score :0.0 |
| Tg exposure induced 7 . 2 fold , 3 . 6 fold and 8 . 8 fold increase in grp 78 , grp 94 and gadd 153 mRNA levels , respectively . ^^^ However , after peroxide pre exposure , the Tg induced effect on grp 78 , grp 94 and gadd 153 mRNA levels was completely blocked . ^^^ |
|
| Interacting proteins: P01266 and P14625 |
Pubmed |
SVM Score :0.0 |
| The mRNA levels of genes responding to conditions of ER stress ( grp 78 , grp 94 , gadd 34 and gadd 153 ) were increased up to 200 fold after Tg exposure , whereas those coding for ER resident proteins ( SERCA 2b and Bcl 2 ) were increased up to 6 fold in treated cultures , and those coding for cytoplasmic proteins ( heat shock protein 70 and p 67 ) were increased only 2 4 fold . ^^^ |
|
| Interacting proteins: P01266 and P14625 |
Pubmed |
SVM Score :0.0 |
| Interestingly , thapsigargin treatment induces the premature exit of Tg from the calnexin / calreticulin cycle , while stabilizing and prolonging interactions of Tg with BiP ( immunoglobulin heavy chain binding protein ) and GRP 94 ( glucose regulated protein 94 ) , two chaperones whose binding is not carbohydrate dependent . ^^^ |
|
| Interacting proteins: P01266 and P14625 |
Pubmed |
SVM Score :0.0 |
| We have previously demonstrated that several endoplasmic reticulum ( ER ) proteins , including BiP , ERp 72 , grp 94 , and protein disulfide isomerase , bind to a denatured thyroglobulin ( Tg ) affinity column and can be specifically eluted by ATP ( Nigam , S . ^^^ Using chemical cross linking , we now demonstrate that BiP , ERp 72 , and grp 94 associate with Tg in two types of cultured thyroid cells , FRTL 5 and PCC 13 . ^^^ Whereas BiP could be coimmunoprecipitated with anti Tg antibodies in the absence of cross linking , only trace amounts of ERp 72 and grp 94 were coimmunoprecipitated . ^^^ Likewise , in both cell types , anti BiP antibodies were able to coimmunoprecipitate Tg in the absence of cross linking , though ERp 72 and grp 94 were only minimally present . ^^^ In contrast , after cross linking , there was a large increase in the amount of ERp 72 and grp 94 that coimmunoprecipitated with anti Tg antibodies , although there was only a slight increase in BiP . ^^^ |
|
| Interacting proteins: P01266 and P14625 |
Pubmed |
SVM Score :0.0 |
| Furthermore , coimmunoprecipitation studies after antimycin A treatment demonstrated that Tg stably associates with BiP , grp 94 , and ERp 72 . ^^^ |
|
| Interacting proteins: P01266 and P14625 |
Pubmed |
SVM Score :0.0 |
| A weak induction of GRP 94 was observed following 6 8 h of continuous exposure to TG . ^^^ |
|
| Interacting proteins: P01266 and P14625 |
Pubmed |
SVM Score :0.0 |
| Immunolocalization of GRP 94 in TG treated cells showed that the protein was transported to the Golgi complex and , in parallel , the KDEL receptor was redistributed from the Golgi to p 58 positive IC structures , but was not transported further to the ER . ^^^ |
|
| Interacting proteins: P01266 and P14625 |
Pubmed |
SVM Score :0.0 |
| RESULTS : Six hours after heat stress the genes whose expression was altered included the FK 506 binding protein gene ( decreased ) , the T complex protein 1 alpha subunit gene ( increased ) , and the 94 kDa glucose regulated protein gene ( increased in uninfected TG , decreased in infected TG ) . ^^^ |
|
| Interacting proteins: P01266 and P14625 |
Pubmed |
SVM Score :0.0 |
| Six hours after heat stress , the genes whose expression was altered included the FK 506 binding protein gene ( decreased ) , the T complex protein 1alpha subunit gene ( increased ) , and the 94 kDa glucose regulated protein gene ( increased in uninfected TG , decreased in infected TG ) . ^^^ |
|
| Interacting proteins: P01266 and P14625 |
Pubmed |
SVM Score :0.0 |
| Inhibition of ER glucosidase activity blocked formation of a subgroup of Tg adducts containing ERp 57 while causing increased Tg adduct formation with protein disulfide isomerase ( PDI ) , delayed adduct resolution , perturbed oxidative folding of Tg monomers , impaired Tg dimerization , increased Tg association with BiP / GRP78 and GRP 94 , activation of the unfolded protein response , increased ER associated degradation of a subpopulation of Tg , partial Tg escape from ER quality control with increased secretion of free monomers , and decreased overall Tg secretion . ^^^ |
|
| Interacting proteins: P01266 and P14625 |
Pubmed |
SVM Score :0.0 |
| When NTG and ATF 6 MER TG mice were treated with or without tamoxifen for 5 days , only the hearts from the tamoxifen treated TG mice exhibited increased levels of many ER stress inducible mRNAs and proteins ; for example , GRP 78 and GRP 94 transcript levels were increased by 8 and 15 fold , respectively . ^^^ |
|
| Interacting proteins: P01266 and P14625 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01266 and P14625 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01266 and P14625 |
Pubmed |
SVM Score :0.0 |
| NA |
|