Pubmed abstracts for Protein-Protein Interaction search result :


Interacting proteins: P13747 and P27797 Pubmed SVM Score :0.63641606
Calreticulin bound as efficiently to peptide loaded MHC class 1 complexes as it did to folding intermediates created in vitro , namely free class 1 HC and empty HC beta ( 2 ) m heterodimers . 0.63641606^^^
Interacting proteins: P13747 and P27797 Pubmed SVM Score :0.0
Further studies on the interaction of HLA E with molecules in the endoplasmic reticulum revealed that HLA E associates with the transporter associated with antigen processing ( TAP ) and calreticulin , and that HLA E expression is TAP dependent and tapasin dependent . ^^^
Interacting proteins: P13747 and P27797 Pubmed SVM Score :0.0
MHC associations of autoantibodies against recombinant Ro and La proteins in systemic lupus erythematosus . ^^^ These results indicated that the preferential formation of Ro and La antibodies was not due to an unspecific stimulatory effect of HLA DR 3 but that the antibody response to certain defined proteins ( 52 kD Ro and La ) was influenced by MHC genes in SLE . ^^^
Interacting proteins: P13747 and P27797 Pubmed SVM Score :0.0
By using a superantigen specific assay , we purified a 28 kDa protein from culture supernatants that stimulated T cells in an MHC class 2 dependent , 5 beta specific manner and designated it SSA , streptococcal superantigen . ^^^
Interacting proteins: P13747 and P27797 Pubmed SVM Score :0.0
The presence of both Ro and La antibodies was strongly associated with the MHC haplotype B 8 C4AQ0 DR 3 DQ2 , the association being strongest for DR 3 . ^^^
Interacting proteins: P13747 and P27797 Pubmed SVM Score :0.0
Expression of Ro / SS A , calreticulin , and MHC class 1 antigens , both in the cytoplasm and on the cell surface , was examined by a fixed cell ELISA , immunofluorescence , and immunoblotting . ^^^
Interacting proteins: P13747 and P27797 Pubmed SVM Score :0.0
Roles for calreticulin and a novel glycoprotein , tapasin , in the interaction of MHC class 1 molecules with TAP . ^^^
Interacting proteins: P13747 and P27797 Pubmed SVM Score :0.0
That SSA position 2 contributes to 5 beta specificity could not have been predicted from functional or crystallographic studies of other SAg and suggests that SSA may adopt unique interactions with TCR and / or MHC class 2 molecules . ^^^
Interacting proteins: P13747 and P27797 Pubmed SVM Score :0.0
The data demonstrate that calnexin and calreticulin chaperones assemble with distinct MHC class 1 assembly intermediates in the ER and show that glycan processing is functionally coupled to release of MHC class 1 proteins from peptide transport molecules . . ^^^
Interacting proteins: P13747 and P27797 Pubmed SVM Score :0.0
Because it seemed unlikely that SSA would deviate significantly from an SAg like topology , we hypothesized that variations in SSA Vbeta determining regions might result from differences in SSA MHC class 2 interactions relative to other SAg during SSA presentation to the TCR . ^^^
Interacting proteins: P13747 and P27797 Pubmed SVM Score :0.0
Class 1 MHC heavy chains associate with many proteins in the endoplasmic reticulum , including TAP , calnexin , calreticulin , and the newly defined tapasin molecule . ^^^
Interacting proteins: P13747 and P27797 Pubmed SVM Score :0.0
US 6 protein is found in complexes with TAP1 / 2 , MHC class 1 heavy chain , beta 2 microglobulin , calnexin , calreticulin , and tapasin . ^^^
Interacting proteins: P13747 and P27797 Pubmed SVM Score :0.0
Newly assembled major histocompatibility complex ( MHC ) class 1 molecules , together with the endoplasmic reticulum chaperone calreticulin , interact with the transporter associated with antigen processing ( TAP ) through a molecule called tapasin . ^^^
Interacting proteins: P13747 and P27797 Pubmed SVM Score :0.0
Prior to loading antigenic peptides , assembled major histocompatibility complex ( MHC ) class 1 molecules associate with the transporter associated with antigen processing ( TAP ) in a complex which also includes calreticulin and a recently described component , tapasin . ^^^
Interacting proteins: P13747 and P27797 Pubmed SVM Score :0.0
The assembly of newly synthesized MHC class 1 molecules within the endoplasmic reticulum and their association with the transporter associated with antigen processing ( TAP ) is a process involving the chaperones calnexin and calreticulin . ^^^ In addition , ER 60 is part of the late assembly complexes consisting of MHC class 1 , tapasin , TAP , calreticulin and calnexin . ^^^
Interacting proteins: P13747 and P27797 Pubmed SVM Score :0.0
Distinct patterns of folding and interactions with calnexin and calreticulin in human class 1 MHC proteins with altered N glycosylation . ^^^ These data may explain previously noted differences between mouse and human class 1 MHC proteins and demonstrate that the location of N oligosaccharides within proteins can influence their folding and interactions with chaperones such as calnexin and calreticulin . . ^^^
Interacting proteins: P13747 and P27797 Pubmed SVM Score :0.0
Calreticulin and calnexin interact with different protein and glycan determinants during the assembly of MHC class 1 . ^^^
Interacting proteins: P13747 and P27797 Pubmed SVM Score :0.0
In humans , after dissociation from calnexin , fully oxidized MHC class 1 heavy chains associate with beta 2 microglobulin ( beta 2m ) and the soluble chaperone calreticulin . ^^^
Interacting proteins: P13747 and P27797 Pubmed SVM Score :0.0
MHC class 1 molecules assemble in the endoplasmic reticulum ( ER ) with chaperones , including calnexin and calreticulin , before binding to the transporter associated with antigen processing ( TAP ) . ^^^
Interacting proteins: P13747 and P27797 Pubmed SVM Score :0.0
Most MHC class 1 molecules in the ER that have not yet acquired peptide are simultaneously bound to the transporter associated with antigen processing ( TAP ) , to the 48 kDa glycoprotein tapasin and to the lectin like chaperone calreticulin , in a multicomponent ' loading complex ' . ^^^ Here , we show that this point mutation also disrupted , directly or indirectly , the interaction between MHC class 1 molecules and calreticulin . ^^^
Interacting proteins: P13747 and P27797 Pubmed SVM Score :0.0
SSA and SEB , however , do not compete for binding on the surfaces of cells expressing MHC class 2 molecules . ^^^ These data provide a structural basis for interpreting the interaction of SSA with HLA DQ molecules as well as a foundation for understanding bacterial superantigen affinities for distinct MHC isotypes . . ^^^
Interacting proteins: P13747 and P27797 Pubmed SVM Score :0.0
In contrast , anti calreticulin only coprecipitated peptide free MHC class 1 molecules . ^^^ This difference in association with peptide loaded class 1 suggests that tapasin functions later than calreticulin during MHC class 1 assembly , and controls peptide loading onto MHC class 1 molecules in the endoplasmic reticulum . . ^^^
Interacting proteins: P13747 and P27797 Pubmed SVM Score :0.0
Calreticulin also participates in the reactions yielding assembly of peptides onto nascent MHC class 1 molecules . ^^^
Interacting proteins: P13747 and P27797 Pubmed SVM Score :0.0
Calreticulin is an endoplasmic reticulum resident molecule known to be involved in the folding and assembly of major histocompatibility complex ( MHC ) class 1 molecules . ^^^ By sequential immunoprecipitation using the conformation independent monoclonal antibody HC 10 , we provided evidence that the cell surface 46 kDa protein co precipitated with calreticulin is unfolded MHC 1 . ^^^ These results show for the first time that after T cell activation , significant amounts of calreticulin are expressed on the T cell surface , where they are found in physical association with a pool of beta 2 free MHC class 1 molecules . . ^^^
Interacting proteins: P13747 and P27797 Pubmed SVM Score :0.0
In beta ( 2 ) m deficient FO 1 cells , we found CD1b heavy chains ( HC ) complexed with the chaperones calnexin and calreticulin , while MHC class 1 HC associated only with calnexin . ^^^
Interacting proteins: P13747 and P27797 Pubmed SVM Score :0.0
To better comprehend at a molecular level the regulation of class 1 assembly , we have assessed the influence of multiple individual amino acid substitutions in the MHC class 1 alpha 2 domain on interaction with TAP , tapasin , and calreticulin . ^^^
Interacting proteins: P13747 and P27797 Pubmed SVM Score :0.0
Surprisingly , the H74L heavy chain was able to enter the defined MHC class 1 pathway and associate with beta2M , calreticulin , tapasin , and TAP . ^^^
Interacting proteins: P13747 and P27797 Pubmed SVM Score :0.0
A later and more prolonged interaction of calreticulin , specifically with assembled , disulfide bonded heavy chain , highlights distinct differences in the roles of these two proteins in the assembly of MHC class 1 molecules . ^^^
Interacting proteins: P13747 and P27797 Pubmed SVM Score :0.0
A heterogeneous expression pattern of MHC class 1 antigens , TAP peptide transporter , proteasome subunits , proteasome activator PA 28 and the chaperones calnexin , calreticulin as well as tapasin was displayed by these tumor cell lines . ^^^ IFN gamma treatment corrected the TAP , LMP and tapasin deficiencies and enhanced the constitutive PA28alpha , LMP 7 , calnexin and calreticulin expression which was accompanied with increased levels of MHC class 1 antigens . ^^^
Interacting proteins: P13747 and P27797 Pubmed SVM Score :0.0
This intermediate complex binds MHC class 1 beta ( 2 ) m dimers , an event accompanied by the loss of calnexin and the acquisition of calreticulin , generating the MHC class 1 loading complex . ^^^
Interacting proteins: P13747 and P27797 Pubmed SVM Score :0.0
Once MHC class 1 heavy chain binds beta ( 2 ) microglobulin ( beta ( 2 ) m ) within the endoplasmic reticulum , an assembly complex comprising the class 1 heterodimer , TAP , TAPasin , calreticulin , and possibly Erp 57 is formed before the binding of high affinity peptide . ^^^
Interacting proteins: P13747 and P27797 Pubmed SVM Score :0.0
We show here that complexes of peptides with heat shock proteins hsp 90 , calreticulin , and hsp 70 are also taken up by macrophages and dendritic cells and re presented by MHC class 1 molecules . ^^^
Interacting proteins: P13747 and P27797 Pubmed SVM Score :0.0
During the assembly of major histocompatibility complex ( MHC ) class 1 molecules transient associations are formed with the endoplasmic reticulum resident chaperones calnexin and calreticulin , ERp 57 oxidoreductase , and also with tapasin , the latter mediating binding of the class 1 molecules to the transporter associated with antigen processing ( TAP ) . ^^^
Interacting proteins: P13747 and P27797 Pubmed SVM Score :0.0
Association of ERp 57 with mouse MHC class 1 molecules is tapasin dependent and mimics that of calreticulin and not calnexin . ^^^
Interacting proteins: P13747 and P27797 Pubmed SVM Score :0.0
An immunodominant MHC class 2 restricted tumor antigen is conformation dependent and binds to the endoplasmic reticulum chaperone , calreticulin . ^^^
Interacting proteins: P13747 and P27797 Pubmed SVM Score :0.0
In this study we have compared the interaction of human MHC class 1 molecules with IgG heavy chain ( HC ) binding protein ( BiP ) , calnexin , calreticulin , tapasin and TAP in beta ( 2 ) microglobulin ( beta ( 2 ) m ) or TAP deficient cells , as well as in wild type B LCL cells . ^^^
Interacting proteins: P13747 and P27797 Pubmed SVM Score :0.0
We tested this approach using calreticulin ( CRT ) , which has demonstrated the ability to enhance MHC class 1 presentation and exhibit an antiangiogenic effect . ^^^
Interacting proteins: P13747 and P27797 Pubmed SVM Score :0.0
However , immunoprecipitation of calreticulin , another member of the MHC 1 assembly complex , coprecipitated TAP 1 at levels comparable to those of uninfected cells . ^^^
Interacting proteins: P13747 and P27797 Pubmed SVM Score :0.0
Assembly and antigen presenting function of MHC class 1 molecules in cells lacking the ER chaperone calreticulin . ^^^ MHC class 1 molecules expressed in a calreticulin deficient cell line ( K 42 ) assembled with beta 2 microglobulin ( beta 2 m ) normally , but their subsequent loading with optimal peptides was defective . ^^^
Interacting proteins: P13747 and P27797 Pubmed SVM Score :0.0
Calreticulin binds to the alpha 1 domain of MHC class 1 independently of tapasin . ^^^ Prior to binding to antigenic peptide , the major histocompatibility complex ( MHC ) heavy chain associates with an assembly complex of proteins that includes calreticulin , tapasin , and the transporter associated with antigen processing ( TAP ) . ^^^ We have also shown that high amounts of wild type tapasin are still unable to associate with MHC class 1 in the absence of the MHC class I / calreticulin interaction , confirming the central role of calreticulin in the formation of the MHC class 1 assembly complex . . ^^^
Interacting proteins: P13747 and P27797 Pubmed SVM Score :0.0
Imidazole and oxazole derivatives 1 to 4 were designed and prepared as dipeptide mimetics to replace the Ser Leu dipeptide sequence of Ro 25 9980 ( Ac ( Cha ) RAMA S L NH 2 ) , a peptidic inhibitor of antigen binding to major histocompatibility complex ( MHC ) class 2 DR molecules linked to rheumatoid arthritis ( RA ) . ^^^
Interacting proteins: P13747 and P27797 Pubmed SVM Score :0.0
Antigenic peptides are loaded onto class 1 MHC molecules in the endoplasmic reticulum ( ER ) by a complex consisting of the MHC class 1 heavy chain , beta ( 2 ) microglobulin , calreticulin , tapasin , Erp 57 ( ER 60 ) and the transporter associated with antigen processing ( TAP ) . ^^^
Interacting proteins: P13747 and P27797 Pubmed SVM Score :0.0
The ability of GRP 94 and calreticulin to elicit CD8+ T cell responses indicates that both proteins bind peptides suitable for assembly on to MHC class 1 molecules . ^^^
Interacting proteins: P13747 and P27797 Pubmed SVM Score :0.0
This review discusses the early stages of MHC class 1 maturation regarding BiP and calnexin association , and differences in MHC class 1 heavy chain ( HC ) interaction with calnexin and calreticulin are highlighted . ^^^
Interacting proteins: P13747 and P27797 Pubmed SVM Score :0.0
The resulting peptides are thought to be translocated into the lumen of the endoplasmic reticulum ( ER ) by specific transporters associated with antigen presentation ( TAP ) , and loaded onto MHC class 1 molecules by a complex `` loading machinery ' ' ( which includes tapasin , calreticulin and Erp 57 ) . ^^^
Interacting proteins: P13747 and P27797 Pubmed SVM Score :0.0
Here we show that early phagosomes from human dendritic cells ( DCs ) contain the peptide loading complex , incorporating MHC class 1 , beta 2 microglobulin , transporter associated with Ag processing ( TAP ) , calreticulin , tapasin , and ERp 57 . ^^^
Interacting proteins: P13747 and P27797 Pubmed SVM Score :0.0
The loading of newly synthesised MHC class 1 molecules ( MHCI ) with peptides requires the involvement of several endoplasmic reticulum ( ER ) resident cofactors including calnexin , calreticulin , transporter associated with antigen processing , ERp 57 and tapasin . ^^^
Interacting proteins: P13747 and P27797 Pubmed SVM Score :0.0
We have previously enhanced DNA vaccine potency by targeting antigen to MHC antigen presentation pathways , using proteins such as Mycobacterium tuberculosis heat shock protein 70 , calreticulin , domain 2 of Pseudomonas aeruginosa exotoxin A , or the sorting signal of the lysosome associated membrane protein type 1 . ^^^
Interacting proteins: P13747 and P27797 Pubmed SVM Score :0.0
Proper assembly of MHC class 1 molecules is dependent on several cofactors , e . g . the chaperones calnexin and calreticulin residing in the endoplasmic reticulum . ^^^
Interacting proteins: P13747 and P27797 Pubmed SVM Score :0.0
A dynamic multimolecular assembly termed the peptide loading complex ( PLC ) participates in this process and is composed of MHC class 1 molecules , calreticulin , ERp 57 , and tapasin bound to the transporter associated with antigen processing ( TAP ) peptide transporter . ^^^
Interacting proteins: P13747 and P27797 Pubmed SVM Score :0.0
Calreticulin has been shown to enhance MHC class 1 presentation of linked peptide / protein and may be useful for antigen specific cancer treatment . ^^^
Interacting proteins: P13747 and P27797 Pubmed SVM Score :0.0
For example , calreticulin , a quality control lectin like chaperone , interacts with glucosylated mannose glycans presented by empty major histocompatibility complex ( MHC ) class 1 molecules , retaining them in the endoplasmic reticulum ( ER ) until antigenic peptide is loaded . ^^^
Interacting proteins: P13747 and P27797 Pubmed SVM Score :0.0
For both of these safeguard mechanisms , the MHC class 1 loading complex ( which consists of the peptide transporter TAP , the chaperones tapasin and calreticulin , and the protein disulfide isomerase ERp 57 ) plays a central role . ^^^
Interacting proteins: P13747 and P27797 Pubmed SVM Score :0.0
The assembly of MHC class 1 molecules with beta ( 2 ) microglobulin and peptides is assisted by the housekeeping chaperones calnexin , calreticulin , and Erp 57 and the dedicated accessory protein , tapasin . ^^^ Tapasin and calreticulin are essential for efficient MHC class 1 assembly , but their precise action during class 1 assembly remains to be elucidated . ^^^ Previous in vitro studies have demonstrated that the lectin calreticulin interacts with monoglucosylated MHC class 1 heavy chains , whatever their state of assembly with light chains and peptide , and inhibits their aggregation above physiological temperature . ^^^
Interacting proteins: P13747 and P27797 Pubmed SVM Score :0.0
Tapasin is the central stabilizer of this complex , which also includes the transporter associated with antigen processing ( TAP ) , MHC class 1 molecules , the ER chaperone , calreticulin , and the thiol oxidoreductase ERp 57 . ^^^
Interacting proteins: P13747 and P27797 Pubmed SVM Score :0.0
This cell line synthesized normal levels of the MHC class 1 H chain and beta ( 2 ) microglobulin , as well as normal levels of TAP , tapasin , GRP 78 , calnexin , calreticulin , ERp 57 , and protein disulfide isomerase . ^^^
Interacting proteins: P13747 and P27797 Pubmed SVM Score :0.0
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