Pubmed abstracts for Protein-Protein Interaction search result :

Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.60907043
The data demonstrate that CD 8 can interact with MHC class 1 molecules expressed on T cells and deliver a costimulatory signal that increases IL 2 production . 0.60907043^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :1.1674288
The data suggest that the mouse CD 8 alpha homodimer does not bind to the alpha 3 domain of HLA class 1 molecules and that CD 8 alpha acts as a co receptor with the TCR by binding the same MHC molecule for alloantigen recognition . 1.1674288^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.51978973
This finding unequivocally establishes that for negative selection in the thymus , CD 8 must interact with the same MHC class 1 molecule as the T cell receptor . . 0.51978973^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.68371072
In this system , CD 8 and class 1 molecules mediate cell cell adhesion , showing that CD 8 directly binds to MHC class 1 molecules . . 0.68371072^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.5319236
Our data suggest that the interaction between CD 8 alpha and the alpha 3 domain of class 1 MHC does not require the participation of neighboring class 1 sequences or beta 2 m . . 0.5319236^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :1.0903002
One such role is the interaction between CD 8 and a conserved region of class 1 MHC , as suggested by the finding that holding the total MHC concentration constant with an irrelevant class 1 MHC molecule ( H 2Kb engineered to have the same alpha 3 domain as H 2Dd ) made the T cell response less sensitive to the change in concentration of the relevant MHC molecule ( H 2Dd ) . 1.0903002^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.67174004
To determine whether recombinant CD 8 can interact with MHC class 1 molecules , we developed an assay that measures binding of MHC class 1 bearing cell lines to purified CD 8 adsorbed to plastic plates . 0.67174004^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.70437174
The CD 8 cDNA contains three mutations : one in the extracellular domain which prevents interaction with its ligand MHC class 1 and the other two in the cytoplasmic domain which inhibit its signalling function . 0.70437174^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.55675833
The cell surface glycoprotein CD 8 functions as a coreceptor with the TCR for interaction with MHC class 1 . 0.55675833^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.56686303
In this review , we summarize the evidence that this immune suppression mediated by CD 8 ( + ) T cells is dependent , in part , on specific cognate interactions between MHC class 1 restricted regulatory CD 8 ( + ) cells and antigen activated CD 4 ( + ) T cells . 0.56686303^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.71468392
All three models demonstrated that lowering CD 8 levels resulted in the requirement for a higher avidity peptide MHC interaction with the TCR to respond equivalently to unmanipulated CD 8 ( high ) T cells of the same specificity . 0.71468392^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.59972389
Thus , the interaction of CD 8 with a nonstimulatory peptide MHC complex helps mediate T cell recognition of antigen , improving the coreceptor function of CD8 . . 0.59972389^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Previous studies showed that a subset of CD 8 ( + ) cytolytic T lymphocytes expressing HLA class 1 specific natural killer inhibitory receptors ( iNKR ) is characterized by the ability to recognize HLA E and to mediate T cell receptor dependent killing of different NK cell susceptible tumor target cells . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
In the periplaque area , numerous CD 2 , CD 4 , and CD 8 positive T cells and cells reactive for the CD45RO phenotype of memory cells and for gamma / delta T cell receptors are located between MHC positive glial cells . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
The TCR and CD 8 complexes of CD8+ T cells bind to different regions of MHC class 1 molecules and both play important roles in the response of the CD8+ T cells to Ag / MHC on APCs . ^^^ In this report , we mimicked common MHC binding with an anti CD 3 : anti CD 8 ( CD 3 , 8 ) BSMAB to isolate the effect of CD 3 : CD 8 pairing , compared this with the effect of CD 3 : CD 3 pairing by the parental bivalent anti CD 3 MAB , and with monovalent anti CD 3 binding by an anti CD 3 : anti CD 4 ( CD 3 , 4 ) BSMAB . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Expression of CD 4 , CD 8 , IL 2 receptor alpha chain ( IL 2R alpha ) , and MHC class 2 ( MHC 2 ) on peripheral blood mononuclear cells were examined in cats infected with feline immunodeficiency virus ( FIV ) . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
We found that the diminished mouse helper T cell responses to xenoantigens result from at least two defects in cell surface molecule interactions between T cells and xenogeneic APCs , specifically TCR and / or CD 8 interactions with xenogeneic class 1 MHC molecules and accessory molecule interactions with their ligands ( probably LFA 1 with ICAM 1 / ICAM 2 and / or LFA 2 with LFA 3 ) . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Most cytotoxic responses ( except those directed against ENV ) were mediated by CD 8 cells and were MHC class 1 restricted . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
These findings suggest that effector cells mediating non MHC restricted cytotoxicity in cattle prevail in a population bearing a CD2+ , CD 4 , CD 8 phenotype and that this population depends on the continuous presence of IL 2 for optimal cytotoxic function . . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Studies with TCR transgenic mice have shown that the accumulation of mature CD4+ and CD8+ thymocytes is strongly skewed by the MHC restriction specificity of the TCR , thus suggesting that commitment of CD4+ CD8+ precursors to the CD 4 or CD 8 lineage is a direct consequence of TCR / MHC interactions . ^^^ These data argue that the early stages of CD 4 and CD 8 lineage development in the thymus are ( at least in part ) controlled by homeostatic mechanisms independent of appropriate TCR / MHC interactions . . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Since the more stringent thymus dependence of CD 8 alpha + beta + T cells may be due to a requirement for thymic selection on self major histocompatibility complex class 1 antigens , the virtually exclusive CD 8 alpha + beta + phenotype of peripheral rat gamma / delta T cells could mean that antigen recognition by this subset is also restricted by MHC class 1 molecules . . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Its fine specificity is identical to that of a classical H Y reactive CD 4 CD8+ MHC class 1 restricted CTL clone , showing that CTL expressing either CD 4 or CD 8 can display identical antigenic specificities . ^^^ The existence of such MHC class 1 restricted mature TcR alpha / beta+ T cells expressing CD 4 and not CD 8 is relevant because it indicates that the generally accepted model for thymic selection , in which the TcR specificity alone determines CD4 / CD8 expression of mature thymocytes , may not be absolute . . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
CD 8 positive T cells surrounded these collections as well as being scattered throughout the membrane and also frequently expressed MHC Class 2 antigens . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
The lysis of the target cells was dependent on a class 1 MHC molecule and the accessory molecule CD 8 . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
We examined the TcR gamma delta usage and co expression of CD 5 , CD 4 , CD 8 and major histocompatibility complex ( MHC ) class 2 on isolated sheep IEL and lamina propria lymphocytes ( LPL ) , and compared them with the TcR gamma delta + cells in peripheral blood , intestinal lymph and jejunal Peyer ' s patches ( PP ) . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
The number of neurons in the sympathetic ganglia was counted and four subpopulations of mononuclear inflammatory cells were identified by monoclonal antibodies MHC 2 , CD 8 T cells / NK cells , CD 5 T cells , CD 4 T cells / macrophages . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
During physiologic activation of mature CD8+ T cells , TCR and CD 8 bind to the same Ag complexed MHC class 1 molecule . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
In the present study the ability of villous chorion as well as fetal cell pulsed early human pregnancy decidual APC to generate selectively antigen non specific and MHC class 2 unrestricted CD 8 positive T suppressor cells was reassessed in view of the fact that placental trophoblast , unlike the fetus , constitutes the fetal tissue of major contact at the maternal fetal interface . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
To determine whether progesterone causes a change in lymphocyte subpopulations in the endometrium , frozen sections of intercaruncular and caruncular endometrium from ewes receiving daily i . m . injections of 100 mg / day progesterone were evaluated by immunohistochemistry for the presence of lymphoid cells bearing CD 45 , major histocompatibility complex ( MHC ) class 2 , CD45R , CD 4 and CD 8 antigens . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Conditioning with monoclonal antibodies ( mAb ) to CD 4 and CD 8 has been sufficient where donor and recipient are mismatched at only multiple `` minor ' ' histocompatibility loci , or at major histocompatibility complex ( MHC ) class 1 plus `` minor ' ' loci , but not where the mismatch involves the entire MHC . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Besides the central role of TCR engagement by MHC in triggering selection ; the interaction of CD 8 and CD 4 with MHC class 1 and class 2 , respectively ; is thought to be important in regulating the selection process . ^^^ We describe that CD 8 and the TCR are selectively associated in thymocytes of mice expressing the restricting MHC , but not in thymocytes of mice expressing a nonrestricting MHC . pp56lck and pp59fyn , the tyrosine kinases associated with CD 8 and TCR , respectively , were found to be present in this complex in an activated form . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Selecting T cell receptors with high affinity for self MHC by decreasing the contribution of CD 8 . ^^^ The affinity of T cell interactions with class 1 MHC molecules includes contributions by both the T cell receptor and the CD 8 coreceptor . ^^^ Therefore , by decreasing the affinity of the interaction with CD 8 , T cells whose receptors have relatively high affinities for self MHC may survive negative selection . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Activation of mature lymphocytes requires in addition to the TCR contact with the corresponding antigen the binding of the CD 8 or CD 4 co receptors to MHC class 1 or class 2 proteins respectively . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Macrophages which are capable of presenting antigen in association with major histocompatibility antigens ( MHC ) class 1 and class 2 to CD 8 ( + ) , and CD 4 ( + ) T cells , respectively , were found in otosclerotic lesions based on their expression of the MAC 387 antigen . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
The GM cells are positive for nonspecific esterase , peroxidase , and MAC 1 markers but are negative for FC gamma receptors and for Thy 1 . 2 , CD 8 , CD 4 , MHC class 2 , and Asialo GM 1 markers . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Beta chain broadens range of CD 8 recognition for MHC class 1 molecule . ^^^ It is known that the alpha chain of CD 8 binds to a negatively charged loop composed of residues 223 to 229 on MHC class 1 Ag and that binding of CD 8 alpha enhances Ag recognition of T cells . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Small CD4+CD8+ cells with high levels of a major histocompatibility complex ( MHC ) class 1 restricted transgenic TcR develop in vitro exclusively into CD 4 CD8+ cells while small CD4+CD8+ TcRhigh cells with heterogeneous TcR from various mice yield both CD 4 and CD 8 T cells . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Five lymphocyte subpopulations , including natural killer cells , the suppressor inducer CD 4 subset , the suppressor precursor CD 8 subset , non major histocompatibility complex ( MHC ) restricted cytotoxic T cells , and CD5+ B cells , were all decreased significantly after plasmapheresis ( p less than 0 . 05 ) . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
The mature T cell receptor ( TCR ) repertoire is established on the basis of discriminative events involving binding of the TCR alpha and beta chains and CD 4 or CD 8 on immature thymocytes to major histocompatibility complex ( MHC ) / self peptide complexes expressed in the thymus . ^^^ To ask whether the strength of the interaction between a CD8 / TCR complex and a MHC / self peptide ligand plays a pivotal role in deciding the fate of a maturing thymocyte , we generated lines of transgenic mice that express distinct and elevated levels of CD 8 alpha , approximately 2 , 3 , and 6 10 times . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Most helper T cells express CD 4 , which binds to class 2 major histocompatibility complex ( MHC ) proteins , and most cytotoxic T cells express CD 8 , which binds to class 1 MHC proteins . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
The phenotypes of isolated cells were characterised for expression of CD 45 , CD 3 , CD 4 , CD 8 , CD 14 , CD 15 , CD 68 , CD 22 , CAM 5 . 2 and class 2 MHC antigens and compared with those in situ in frozen sections of the same placentas . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Mice given a single short course of anti CD 4 and anti CD 8 monoclonal antibodies ( mAb ) became tolerant of major histocompatibility complex ( MHC ) incompatible vascularized heart allografts in a donor and organ specific manner . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Several amino acids considered to be important for the interaction of the T cell coreceptor CD 8 with the MHC class 1 alpha 3 domain are conserved in the shark sequence . . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Using a panel of monoclonal antibodies ( mAbs ) , we have examined the time course of expression of major histocompatibility complex ( MHC ) antigens class 1 ( OX 18 ) and class 2 ( OX 6 ) , leukocyte common antigen ( OX 1 ) , CD 4 ( W3 / 25 ) and CD 8 ( OX 8 ) antigens , CR 3 complement receptor ( OX 42 ) , as well as brain macrophage antigen ( ED 2 ) . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
MHC class 2 interaction with CD 4 mediated by a region analogous to the MHC class 1 binding site for CD 8 . ^^^ Interactions between major histocompatibility complex ( MHC ) molecules and the CD 4 or CD 8 coreceptors have a major role in intrathymic T cell selection . ^^^ Physical interaction between the CD4 / MHC class 2 molecules and CD8 / MHC class 1 molecules has been demonstrated by cell adhesion assay , and a binding site for CD 8 on class 1 has been identified . ^^^ Here we demonstrate that a region of the MHC class 2 beta chain beta 2 domain , structurally analogous to the CD 8 binding loop in the MHC class 1 alpha 3 domain , is critical for function with both mouse and human CD4 . . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
The ability of monoclonal antibodies against CD 4 and CD 8 to block antigen recognition by T cells , as well as cell cell adhesion assays , indicate that CD 4 and CD 8 bind to nonpolymorphic determinants of class 2 or class 1 MHC . ^^^ These findings show that CD 4 interacts with a region of MHC class 2 molecules analogous to a previously identified loop in class 1 MHC proteins that binds CD 8 ( refs 8 , 9 ) . . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Differentiation antigens evaluated included cluster of differentiation antigens 2 , 4 , and 8 ( CD 2 , CD 4 , and CD 8 , respectively ) ; B cells ; and major histocompatibility complex ( MHC ) class 2 , DQ , and DR . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
CD 8 expression alters the fine specificity of an alloreactive MHC class 1 specific T hybridoma . ^^^ The influence of CD 8 on the fine specificity of MHC class 1 restricted T cell allorecognition was evaluated by comparing the reactivity of CD 8 and CD 8 transfected forms of an allospecific , H 2Kb restricted T hybridoma . ^^^ These findings reflect an important role for CD 8 in influencing the fine specificity of MHC class 1 recognition by T cells and may indicate a limited structural role for peptide antigen in defining the ligand recognized by these alloreactive T cells . . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
CD 4 and CD 8 may adopt a similar mode of binding to their MHC ligands . ^^^ A theoretical scheme is proposed by which the type specific cell surface receptors of T lymphocytes , CD 8 and CD 4 , bind class 1 and 2 MHC proteins in a similar manner . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
The monomorphic cell surface glycoprotein CD 8 acts as co receptor in the recognition of peptide major histocompatibility complex ( MHC ) class 1 complexes by cytotoxic lymphocytes ( CTL ) by binding to the monomorphic alpha 3 domain of the class 1 molecule . ^^^ Recent reports suggest there is no interspecies recognition between CD 8 and MHC class 1 . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Species specificity in the interaction of CD 8 with the alpha 3 domain of MHC class 1 molecules . ^^^ The alpha 1 and alpha 2 domains of the class 1 MHC molecule constitute the putative binding site for processed peptides and the TCR , although the alpha 3 domain has been implicated as a binding site for the CD 8 molecule . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
We have studied T cell receptor ( TCR ) and alpha / alpha CD 8 expression in thymus independent intraepithelial lymphocytes ( TI IELs ) from the gut of mice bearing transgenic ( TG ) TCR alpha beta specific for the male antigen , presented by H 2Db class 1 major histocompatibility complex ( MHC ) molecules . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
The interaction of the T cell surface glycoprotein CD 8 with major histocompatibility complex ( MHC ) class 1 molecules on target cells is required for effective T cell activation . ^^^ Mutations in the alpha 3 domain of the MHC class 1 molecule can disrupt binding to CD 8 , yet leave antigen presentation unaffected . ^^^ Endogenous MHC class 1 molecules were competent to bind CD 8 , but were unable to rescue the defect , indicating a requirement for coordinate recognition of antigen / MHC by a complex of the TCR and CD 8 coreceptor for both positive and negative selection of thymocytes . . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
The immune response to CML may be mediated by major histocompatibility ( MHC ) locus unrestricted natural killer and lymphokine activated killer cells , or by MHC restricted CD 4 and CD 8 lymphocytes . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
The human CD 8 molecule is a T cell specific surface receptor that is intimately involved in class 1 MHC restricted Ag recognition and subsequent T cell activation . ^^^ CD 8 expressed in splenic T cells associated with the lymphoid specific tyrosine protein kinase p56lck , participated in T cell activation and conferred an increased xenogeneic response to human MHC class 1 Ag . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
We speculate that the latter region may contain the binding site of CD 8 alpha to the alpha 3 domain of major histocompatibility complex ( MHC ) class 1 molecules . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
MAbs to CD 3 , CD 8 , and MHC class 1 antigens blocked the lysis of LCLs by HLA specific and LCL selective CTLs with comparable efficiency suggesting that the two effector types express T cell receptors of similar affinity . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
MHC class 1 molecules occupied with irrelevant peptides ( a majority present on RMA , largely absent on RMA S ) may interfere in the interaction of the CD 8 molecule with relevant MHC / peptide complexes . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
These data suggest that CD 8 is necessary for the maturation and positive selection of class 1 MHC restricted cytotoxic T lymphocytes but is not required on any of the intermediate thymocyte populations ( CD8+CD4 TcR or CD4+CD8+TcRlow ) during the development of functional class 2 MHC restricted helper T cells . . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
In contrast , 7 to 50 % of mAb to CD 2 , CD 4 , CD 8 , CD11a / CD18 , CD 44 , and class 1 MHC molecules were resistant to detergent solubilization , demonstrating that a fraction of these molecules was constitutively associated with the cytoskeleton . ^^^ In addition , cross linking increased the fraction of CD 2 , CD 4 , CD 8 , CD11a / CD18 , CD 44 , and class 1 MHC molecules that was associated with the cytoskeleton . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
We found that CD 8 depleted mice did reject MHC class 1 disparate skin grafts , but only when the grafts also expressed additional alloantigens . ^^^ These CD8+ CTL effectors were unique in that : ( a ) their generation was dependent upon the injected anti CD 8 mAb and upon exposure to class 1 MHC alloantigens expressed on the engrafted skin , and ( b ) their effector function was resistant to blockade by anti CD 8 mAb . ^^^ We observed that the additional alloantigens coexpressed on MHC class 1 disparate grafts that triggered graft rejection in CD 8 depleted mice could be MHC linked or not and that they functioned in these rejection responses to activate third party specific CD4+ T helper ( Th ) cells to provide helper signals for the generation of CD8+ anti CD 8 resistant CTL effector cells . ^^^ Thus , mice depleted of CD8+ T cells by thymectomy and in vivo administration of anti CD 8 mAb harbor a unique population of anti CD 8 resistant , CD8+ effector cells that mediate anti MHC class 1 responses in vivo and in vitro , but require help from third party specific Th cells to do so . . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
However , anti CD 8 antibodies co precipitated class 1 MHC heavy chain and associated beta 2 microglobulin in all conditions . ^^^ The latter co precipitation was shown to result from `` cis type ' ' interactions between CD 8 and class 1 MHC proteins on the same cell and to involve a degree of selectivity , as class 1 MHC molecules were absent from immunoprecipitates of highly expressed cell surface molecules such as LFA 1 . ^^^ A possible role for `` cis type ' ' class 1 MHC CD 8 interactions in the dynamics of CTL target cell contacts is proposed . . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Virus entry , uncoating and new viral protein synthesis can load both the cytosolic and the endosomal pathway of antigen processing , resulting in viral peptide presentation to CD 8 and CD 4 T cells by MHC class 1 and 2 molecules , respectively . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Furthermore , wild type and altered CD 8 alpha exhibited similar ability to be capped and to induce co capping of TCR and MHC ( major histocompatibility complex ) class 1 : the fraction of cell surface label redistributed into a localized cap ranged between 40 % and 80 % . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Studies in transgenic and inbred strains of mice have shown that the critical molecular interactions controlling positive selection involve major histocompatibility complex ( MHC ) , T cell receptor ( TCR ) , and CD 4 or CD 8 coreceptor molecules . ^^^ Correlations have been established between MHC gene products and the percentage of CD 4 or CD 8 T cells that express specific variable ( 5 ) beta gene products as part of the alpha beta heterodimer . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
However , neither the CD 4 nor the CD 8 ( T cytotoxic ) antigens were involved in the binding process , and while the fibroblasts expressed Class 1 MHC molecules ( but not Class 2 ) , these also had no role in mediating lymphocyte adhesion . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
A second family of adhesion receptors is the immunoglobulin superfamily , members of which include CD 2 , LFA 3 and ICAM 1 , which participate in T cell adhesive interactions , and the antigen specific receptors of T and B cells , CD 4 , CD 8 and the MHC Class 1 and 2 molecules . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
CD 8 is a heterodimeric membrane glycoprotein on MHC class 1 restricted T lymphocytes that cooperates with the alpha beta CD 3 TCR in the recognition of MHC class 1 molecules presenting antigenic peptides . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Whereas CD 7 expression was increased 78 + / 25 % by 0 . 5 microM ionomycin , expression of CD 25 ( IL 2R alpha ) , class 2 MHC , 4F2 , transferrin receptor , CD 2 , CD 3 , CD 4 , CD 5 , and CD 8 molecules was not increased . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Monoclonal antibodies ( MAb ) against classes 1 and 2 antigens of the major histocompatibility complex ( MHC ) and their receptors , CD 8 and CD 4 , had no effect on T cell interaction with SSc and normal cells , while MAb against lymphocyte function associated antigen type 3 ( LFA 3 ) and CD 2 both strongly inhibited lymphocyte attachment . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
To formally establish that this pattern of exogenous Ag processing requires the presence of a class 1 MHC product , we demonstrated that beta 2 microglobulin deficient macrophages , which lack class 1 MHC product expression , can not present HKLM to CD 8 cells . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
The phenotype and activation status of 5000 TILs were studied using Mabs to CD 4 , CD 8 , HLA DR , CD 25 ( the low affinity inducible IL 2 receptor ) and the transferrin receptor and related to Class 1 and Class 2 MHC expression on 5000 primary tumour cells . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
HLA class 1 ( A , B , C ) and Class 2 ( DR , DQ ) products of the major histocompatibility complex ( MHC ) , and the percentages of CD 3 , CD 4 and CD 8 T cell subsets were investigated . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
T cell fate is determined primarily by the nature of the interaction between a complex composed of the T cell receptor and CD 4 or CD 8 molecules on the T cell surface and the peptide antigens that are bound to MHC products and are displayed by other nonlymphoid cells present in the thymus . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Reactivity with the following antigens was assessed : MHC class 1 , 2 DR , DQ , and DP antigens ; leukocyte markers CD 1 , CD 2 , CD 3 , CD 4 , CD 8 , CD 14 , CD 16 , CD 25 , CD 57 ; a proliferation associated nuclear antigen ; interferon gamma and tumor necrosis factor alpha . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Furthermore , CD8+ T cell responses to Mls 1a / class 2 MHC do not require ( and are in fact inhibited by ) the presence of functional CD 8 molecules . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Eimerian infection induced changes in the intestinal intraepithelial lymphocyte ( IEL ) subpopulations expressing CD 8 antigen ( cytotoxic / suppressor T cells ) or antigen specific T cell receptor ( TCR ) heterodimer alpha beta ( TCR 2 ) or gamma delta ( TCR 1 ) were investigated in F 2 crosses of 15I5 B congenic chickens differing for the major histocompatibility complex ( MHC ) . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
We used immunofluorescence or immunoperoxidase techniques to study frozen sections of muscle from our patient , who had mild weakness of cervical and proximal limb muscles , and from control patients with polymyositis , inclusion body myositis , dermatomyositis , or granulomatous myopathy with monoclonal antibodies against T cell related antigens ( CD 2 , CD 3 , CD 4 , CD 8 , and gamma / delta T cell receptor ) , B cells ( CD 22 ) , major histocompatibility complex ( MHC ) and MHC related antigens ( MHC Class 1 , CD1a , CD1b , and CD1c ) , and the 65 kd heat shock protein . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
As opposed to human T lymphocytes , expression of porcine MHC 2 is not transient and restricted to lymphoblasts but is imminent in small , resting T lymphocytes of the two CD 8 expressing subsets , even though also in swine activation can induce MHC 2 . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
The CD 4 and CD 8 antigens on the surface of T cells appear to bind to major histocompatibility complex ( MHC ) class 2 and 1 antigens , respectively . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Taken together , our data suggest a model of thymic development in which CD 4 or CD 8 may enhance TCR / CD3 signaling upon coligation by an MHC molecule . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
The immunolocalization of CD 3 , CD 4 , CD 8 , CD 22 , and CD 56 markers , the gamma delta T cell receptor , major histocompatibility complex ( MHC ) class 1 complex and class 2 antigens , and complement membrane attack complex ( MAC ) were examined . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
The specificity of the T cell antigen receptor ( TCR ) for class 1 or class 2 MHC influences the commitment to a CD 4 or CD 8 lineage . ^^^ This outcome is consistent with a model for positive selection in which selection occurs at a developmental stage in which both CD 4 and CD 8 are expressed , and positive selection by class 1 MHC generates an instructive signal that directs differentiation to a CD 8 lineage . . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
The commitment to the CD 4 and CD 8 lineage depends on the binding of the alpha beta TCR to thymic major histocompatibility complex ( MHC ) coded class 2 and class 1 molecules , respectively . ^^^ The selective model predicts that a CD 8 transgene which is expressed in all immature and mature T cells should rescue CD4+ class 1 MHC specific T cells from cell death . ^^^ We have performed experiments in CD 8 transgenic mice which fail to support a selective model and we present data which show that the binding of the alpha beta TCR to thymic class 1 MHC molecules results in up regulation of the TCR in the CD4+8+ population . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
The human CD 8 glycoprotein functions as a co receptor during T cell activation by both binding to MHC class 1 and transducing a transmembrane signal . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Dinucleotide repeat polymorphism at the human MHC class 1 CD 8 alpha chain ( Leu 2 / T8 ) gene . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
CD 8 is a membrane glycoprotein of 34 kd on cytotoxic / suppressor T lymphocytes and is an endogenous ligand for MHC class 1 proteins on target cells . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
The lymphocyte differentiation marker CD 8 acts as a coreceptor with the T cell receptor ( TCR ) during recognition of peptide presented by major histocompatibility complex ( MHC ) class 1 molecules . ^^^ The functions of CD 8 in the TCR complex are thought to be signaling through the association of CD 8 with the protein tyrosine kinase p56lck and adhesion to MHC class 1 through the alpha 3 domain . ^^^ While the ability of the CD 8 alpha / alpha homodimer to bind to classical MHC class 1 molecules has been shown , it is unclear whether CD 8 can also bind nonclassical molecules . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
CD 4 and CD 8 T cell surface molecules play a role in T cell recognition and activation by binding to their respective class 2 and class 1 major histocompatibility complex ( MHC ) ligands on an antigen presenting cell ( APC ) . ^^^ Though CD 4 and CD 8 are capable of binding to MHC molecules in the absence of the T cell receptor ( TCR ) , increasing evidence suggests that they may primarily function by complexing with the TCR to form a ' co receptor ' for recognition of antigen bound MHC . ^^^ Using gene transfer studies we have demonstrated that CD 4 and CD 8 can augment antigen induced IL 2 production through different mechanisms dependent on whether or not they can bind MHC independently of the TCR or complexed with the TCR . ^^^ Under circumstances where CD 4 and CD 8 can bind to the same MHC ligand as the TCR , they potentiate antigen induced IL 2 production maximally by a mechanism in large part dependent on their cytoplasmic tails . ^^^ Enhancement of antigen induced IL 2 production can also occur under circumstances where CD 4 and CD 8 bind on MHC ligand distinct from that recognized by the TCR . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Accumulating data indicate that p56lck transduces intracellular tyrosine protein phosphorylation signals upon engagement of CD 4 and CD 8 by major histocompatibility complex ( MHC ) determinants expressed on antigen presenting cells ( APCs ) . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
In contrast to other accessory molecules , the ligands of CD 4 and CD 8 are the same MHC molecules which are recognized by the T cell receptor . ^^^ The structural analysis of the interaction between CD 8 , CD 4 and their respective ligands , namely class 1 and class 2 molecules of the MHC , will be treated in this review . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Processing of endogenously synthesized proteins generates short peptides that are presented by MHC class 1 molecules to CD 8 T lymphocytes . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Both the subset specific , CD 4 and CD 8 T cell accessory molecules and the antigen specific T cell receptor ( TcR ) interact with major histocompatibility complex ( MHC ) class 1 and class 2 molecules on the surface of antigen presenting cells . ^^^ We utilized a CD 4 , CD 8 , class 1 allospecific T cell hybridoma which functionally manifests both cytotoxic T lymphocyte ( CTL ) and T helper 1 ( Th 1 ) phenotypes , and rendered it bispecific by transfecting it with genes encoding either a class 2 restricted , 2 , 4 , 6 trinitrophenyl ( TNP ) 1 Ad specific TcR or a non MHC restricted chimeric TcR , composed of a variable part of an anti TNP antibody . ^^^ Expression of either CD 4 or CD 8 transgenes in these hybridomas enhanced and augmented their reactivity towards the appropriate target cells regardless of the type of TcR MHC interaction . ^^^ Anti CD 4 or CD 8 antibodies completely inhibited the activity of transfectants expressing the corresponding accessory molecule , regardless of the MHC type of the TcR interaction . ^^^ Taken together , these studies demonstrate that the CD8 / CD4 molecules can exert their accessory function through interactions with MHC molecules which are not directly associated with the TcR Ag MHC complex , and that this accessory effect is associated with TcR mediated triggering at an early stage of the signaling process and is not related to the effector mechanism assigned to the CD 4 and CD 8 T cell subsets . . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
CD8+ cell mediated anti EC cytotoxicity is directed toward allogeneic major histocompatibility complex ( MHC ) class 1 determinants on ECs and involves recognition by the CD3 / T cell receptor complex and the CD 8 molecule on the effector T cells . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Based on the established role for CD 8 in binding to class 1 MHC molecules , we suggest that increased expression of 5 alpha 12 . 1 on CD8+ T cells points to a role for TCR ' s using 5 alpha 12 . 1 in class 1 MHC / Ag recognition . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Although such Ts do not have discernible cytolytic activity , like classical cytotoxic T cells ( Tc ) they express CD 3 and CD 8 on their surface and function in a class 1 MHC restricted manner . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Interestingly , however , T cells expressing 5 beta 5 . 1 , like those expressing 5 beta 17a and 5 beta 11 , are clonally deleted from both CD 4 and CD 8 subsets in mice that express MHC IE molecules . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
The slope analysis of B 6 allo MLR responses to MHC class 1 disparate bm 1 and class 2 disparate bm 12 revealed the presence of a T T cell interaction between CD 4 and CD 8 T cell subsets . ^^^ It was further indicated that CD 4 and CD 8 T cells recognize both class 1 and class 2 MHC . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Tumor cell CTL activity for both long term TIL lines is inhibited by several mAbs , including those against CD 3 , CD 8 , and class 1 MHC molecules , indicating that the effector cells are class 1 restricted CD8+ , CTL . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Morphometric analysis revealed significant increases in numbers of cells expressing CD 5 , CD 4 , CD 8 , CD45R ( mainly B cells ) , major histocompatibility complex ( MHC ) class 2 antigens , Fc receptors ( FcR ) on macrophages ( VPM 32 ) and FcR on B cells and macrophages ( VPM 33 ) from five days post infection . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
As class 1 MHC is the ligand for the CD 8 molecule , expression of CD 8 induced by IL 4 on CD4+ T cells may allow for increased nonspecific cell to cell contact during the course of an inflammatory response . . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
The CD 8 glycoprotein is expressed by thymocytes , mature T cells and natural killer ( NK ) cells and has been implicated in the recognition of monomorphic determinants on major histocompatibility complex ( MHC ) Class 1 antigens , and in signal transduction during the course of T cell activation . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Differential expression of CD 8 alpha and CD 8 beta associated with MHC restricted and non MHC restricted cytolytic effector cells . ^^^ The differential expression of the alpha and beta chains of the CD 8 glycoprotein was examined in three functionally distinct cytolytic effector cell populations : ( 1 ) T cells ( CD3+ CD 56 ) , ( 2 ) NK cells ( CD56+ CD 3 ) , and ( 3 ) non MHC restricted T cells ( CD56+ CD3+ ) . ^^^ In comparison , 75 % of non MHC restricted T lymphocytes were CD8+ but they displayed an intermediate phenotype : 60 % coexpressed CD 8 alpha and CD 8 beta while 40 % expressed only CD 8 alpha . ^^^ Following IL 2 activation , enhancement of non MHC restricted cytotoxicity was not associated with any changes in either the quantitative or qualitative pattern of expression of CD 8 alpha or CD 8 beta by these cells . ^^^ Addition of either anti CD 8 alpha or anti CD 8 beta mAb did not alter non MHC restricted cytotoxicity of either CD56+ CD 3 or CD56+ CD3+ effector cells . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Analysis of the antigen reactivity of the hybridomas revealed that the human CD 8 alpha chain failed to increase the mouse T cell receptor class 1 MHC interaction , even as a heterodimeric form with mCD 8 beta molecules . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
We raise the possibility that TCR 5 beta 8 . 1+CD4 CD 8 thymocytes are derived from TCR 5 beta 8 . 1+CD4+CD8+ thymocytes , and that the reactivity of TCR 5 beta 8 . 1 with both MHC classes 1 and 2 has resulted in the down regulation of both CD 4 and CD8 . . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Surprisingly , despite their MHC specificity , several clones expressed CD 8 rather than CD 4 , but were not cytotoxic . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Tolerance was obtained to minor ( non MHC ) transplantation antigens without T cell depletion even in actively sensitized mice , or to MHC plus minor antigens presented directly by skin grafts using combinations of depleting followed by blockading CD 4 and CD 8 antibodies . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Based on functional tests and double staining experiments , we propose that CD4+ / CD8+ thymocytes enter the TNC where they may undergo positive selection for MHC restriction and further differentiation to CD 4 or CD 8 single positive cells . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Flow cytometric analysis revealed that bone marrow derived suppressor cells are strongly positive for Thy 1 antigen but negative for CD 3 , CD 4 ( L3T4 ) , and CD 8 ( Lyt 2 ) surface markers , and express only class 1 MHC antigens . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
CTLs bearing the surface molecule CD 8 recognize small ( approximately 10 amino acid ) viral peptides that are presented in association with major histocompatibility ( MHC ) class 1 molecules at the surface of tumor cells . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Finally , other non organ specific lymphocyte adhesion molecules for EC may include CD 4 and CD 8 ( which bind to class 2 and class 1 MHC antigens , respectively ) , and CD 2 ( which binds to LFA 3 ) . . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
A second type of T cell lacking CD 4 and CD 8 recognized a distinct B chain determinant of insulin in a class 2 dependent , but MHC unrestricted , fashion . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
The Ag independent homotypic adhesion induced by mAb L 25 was not inhibited by mAb to the lymphocyte function associated Ag 1 ( CD11a / CD18 ) , CD 2 , CD 4 , and CD 8 , or to their ligands ICAM 1 , LFA 3 , MHC class 1 , or MHC class 2 . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Coimmobilization of antibodies specific for any of a variety of CTL surface proteins , including CD 8 , class 1 MHC proteins , CD 45 ( T 200 ) and Thy 1 , had the effect of decreasing the critical threshold density of Ag necessary to trigger responses , and decreasing by an order of magnitude the density required to stimulate a half maximal response . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Peptides bound to class 1 or class 2 major histocompatibility complex ( MHC ) encoded molecules are ligands for the antigen specific T cell receptor of T cells carrying the CD 8 and CD 4 antigens , respectively . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
The function of the T cell differentiation antigens CD 4 ( Leu 3 / T4 ) and CD 8 ( Leu 2 / T8 ) on human cytotoxic T lymphocytes ( CTL ) is presently seen only in conjugate formation between CTL and target cell via class 2 or class 1 MHC antigens rather than in the later killing steps . ^^^ These results are incompatible with an exclusive role of the CD 4 and CD 8 molecules in MHC class recognition and are discussed with respect to a function as negative signal receptors for these molecules on CTL . . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
In the present study , we have analyzed time courses of both quantity and density of the T cell differentiation antigens , CD 3 ( T 3 ) , CD 4 ( T 4 ) , and CD 8 ( T 8 ) , as well as MHC antigens , on the cell surface of T cells , and made correlated measurements of DNA content with the surface antigen quantity as well with RNA content and cell size following activation of T cells by phytohemagglutinin . ^^^ Cell cycle studies demonstrated that the density of class 1 MHC , CD 3 , CD 4 , and CD 8 antigens was greater in G0 / G1 phase cells than G 2 phase cells at all times tested during T cell activation . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
The CD 4 subset of cells displays helper / inducer activity and recognizes class 2 antigens of the major histocompatibility complex ( MHC ) , while the CD 8 subset recognizes class 1 MHC antigens and exhibits cytotoxic or suppressor function . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
The results of our study support the hypothesis that CD 8 is required for recognition of class 1 MHC alloantigens that results in activation of T cell functional responses . . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
It has been suggested that the CD 8 molecule augments T cell avidity for the target cells by binding to determinants on target cell MHC class 1 molecules . ^^^ These experiments provide direct evidence for an interaction of the CD 8 molecule with class 1 MHC proteins as well as between CD 2 proteins and LFA 3 proteins , thus supporting the hypothesis that these molecules can mediate cell cell adhesion . . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
The CD 8 ( Lyt 2 ) molecule is a phenotypic marker for T lymphocytes that recognize and react with major histocompatibility complex ( MHC ) class 1 molecules . ^^^ Antibody blocking experiments and gene transfection studies indicate that CD 8 binds to a determinant on MHC class 1 molecules on the target cells , facilitating interaction between effector T lymphocytes and the target cell . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
A CD 8 ( + ) , MHC class 2 specific CTL clone , was used to show differential inhibition by anti CD 8 mAb , depending on the target cell , therefore providing evidence that anti CD 8 mAb binding does not generate an absolute off signal . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
The majority of CD4+ CD8+ cortical type thymocytes expressed low levels of class 1 MHC Ag , the previously described CD4+ CD8+ thymocyte subpopulation with low CD 8 expression exhibited intermediate levels of class 1 MHC , whereas most of the single positive CD 4 and CD 8 thymocytes displayed high levels of class 1 MHC . ^^^ Chemical cross linking and peptide mapping studies clearly identified the CD 8 associated protein on thymic clones as the class 1 MHC molecule . . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Furthermore , the presence of the transfected CD 8 molecule in the HTL 10 BW hybridomas was found to be inhibitory to class 2 MHC restricted antigen reactivity . ^^^ These results demonstrate that , besides its role in increasing the overall avidity of T cell class 1 MHC / antigen interaction , the CD 8 molecule inhibits T cell class 2 MHC gene product / antigen interaction . ^^^ This negative effect of the CD 8 molecule on a class 2 MHC restricted response may account for the failure of CD8+ T cells using either 5 beta 8 . 1 or 5 beta 6 , which impart reactivity to the Mlsa antigen on CD4+ T cells , to respond to the Mlsa antigen . . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
The relationship between surface molecule expression and encephalitogenicity of myelin basic protein ( BP ) sensitized cells induced by three different sensitization protocols was studied using adoptive transfer in Lewis rats . ( 1 ) In BP / CFA sensitization , CD4+ blastoid T cells showing marked upregulation of CD 4 , class 1 and 2 MHC , and IL 2 receptor molecules , but not CD 5 , CD 8 , or CD 45 , were generated after culture with BP . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
MHC unrestricted transfer of antilisterial immunity by freshly isolated immune CD 8 spleen cells . ^^^ Immune CD 8 cells , which play an essential role in the adoptive transfer of antilisterial immunity , can specifically lyse Listeria bearing macrophages in vitro in an MHC unrestricted manner . ^^^ In sum , our data establish that Listeria immune CD 8 cells can function in vivo in MHC incompatible hosts , and indirectly support the hypothesis that the destruction of infected phagocytes may be important in T cell mediated immunity against Listeria and perhaps other intracellular pathogens . . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
The experiments reported in this paper examine the cell surface phenotype ( CD 5 , CD 4 , CD 8 , T 19 , MHC class 2 and sIg ) and cell output of lymphocyte subsets circulating through a subcutaneous lymph node in the sheep fetus , in an environment unaffected by foreign antigen and circulating immunoglobulins . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Monoclonal antibodies ( MoAbs ) that block interaction with class 1 MHC molecules at the level of target cell ( W6 / 32 anti HLA class 1 ) or effector cell ( CD 8 ) do not inhibit killing by CD 16 CD3+ cells . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
The CD 8 Ag is a cell surface heterodimer which demarcates predominantly cytotoxic T cells which are restricted by class 1 MHC Ag . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
These findings agree well with the assumption that CD 8 positive cells identify the foreign antigen in the context of class 1 antigens , whereas CD 4 positive cells are found to be associated with the recognition of MHC locus 2 coded HLA antigens . . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
The CD 4 and CD 8 glycoproteins play an important role in T cell activation by binding to major histocompatibility complex ( MHC ) class 2 or class 1 molecules , respectively , and stabilizing their interactions with the T cell receptor CD 3 complex during antigen presentation . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Comparison of phosphorylation and internalization of the antigen receptor / CD3 complex , CD 8 , and class 1 MHC encoded proteins on T cells . ^^^ We analyzed the phosphorylation and the dynamics of TCR / CD3 , CD 8 and MHC class 1 molecules during the activation of a CD8+ cytotoxic T lymphocyte clone and of CD 8 T helper hybridomas transfected with the gene coding for the native ( J . ^^^ Constitutive phosphorylation of the MHC class 1 products was found to be intrinsic to the transmembrane / cytoplasmic portion of the molecules because it was transferred to the CD 8 alpha hybrid molecules composed of extracellular CD 8 and MHC class 1 transmembrane and intracytoplasmic domains ( CD 8 e / MHC t 1 ) . ^^^ The same ligand bound to the CD 8 e / MHC t 1 hybrid molecule was internalized at the same rate as a class 1 MHC molecule ligand , indicating that the behavior of the hybrid molecule was characteristic of the transmembrane / cytoplasmic portion of MHC class 1 molecules . . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Furthermore , a blocking study of TcHMC 2 cytotoxicity using monoclonal antibodies against CD 3 , CD 8 and human MHC class 1 products showed that all of these antigen molecules were involved in the cytotoxicity of TcHMC 2 clone against autologous HMC 2 cells , indicating MHC class 1 recognitive cytotoxicity . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Interactions between surface molecules on thymocytes ( TCR , CD 4 , and CD 8 ) and thymic stromal cells ( MHC proteins ) are critical to repertoire selection . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
The CD 4 and CD 8 antigens on the surface of T cells appear to bind to major histocompatibility complex ( MHC ) class 2 and 1 antigens , respectively . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
CD 8 molecules expressed on the surface of a subset of T cells participate in the selection of class 1 MHC antigen restricted T cells in the thymus , and in MHC restricted immune responses of mature class 1 MHC antigen restricted T cells . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
These strongly IFN gamma immunoreactive fibres also expressed antigen defining T cells of the cytotoxic / suppressor phenotype ( CD 8 ) and major histocompatibility complex ( MHC ) class 1 antigen . . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
The expression of the cell surface glycoproteins CD 4 and CD 8 on functionally mature T cells is usually mutually exclusive and correlates with class 2 and class 1 major histocompatibility complex ( MHC ) restriction , respectively . ^^^ CD 4 and CD 8 function by binding to class 2 and class 1 MHC molecules on the antigen presenting cell ( APC ) , thereby increasing the adhesion between the T cell and the APC . ^^^ Therefore , CD 4 and CD 8 do not distinguish between class 1 and class 2 restricted TCRs in their ability to form membrane complexes , indicating a need for both the TCR and its associated accessory molecule to recognize the same individual MHC molecule on the APC to optimize TCR triggering . . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Generation of syngeneic anti tumour double negative cells ( CD 4 CD 8 ) , with cytotoxic activity against clones of different MHC class 1 expression . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
DNA synthesis was abrogated by a mAb to the FcR but not by mAb to other molecules used in clonally specific antigen recognition , i . e . , class 1 and 2 MHC products or CD 4 and CD 8 . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
The majority of peripheral T cells express a heterodimeric , alpha / beta T cell receptor , which recognizes specific antigenic peptides bound to self major histocompatibility complex ( MHC ) molecules , and either the CD 4 or CD 8 surface markers . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Also , CD 4 and CD 8 can serve as adhesion molecules by binding to monomorphic determinants expressed on class 2 and class 1 MHC antigens , respectively , on the target cells , but compared to LFA 1 and CD 2 antigens their contribution to conjugate formation is minor . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
The lytic activity of most CD8+ MHC class 1 allospecific CTL generated in vitro can be inhibited by anti CD 8 antibodies . ^^^ We have recently reported that multivalent cross linking of CD 8 on CTL precursors in MLC does appear to mediate activation signals , and induces the generation of CD8+ MHC class 1 allospecific CTL whose lytic activity can not be blocked by anti CD 8 antibodies . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
The origins of `` help ' ' in rejection of syngeneic tumors by the CD 8 T cell lineage was examined with a model tumor inappropriately expressing novel class 1 MHC and subject to cytolytic T cell ( CTL ) mediated rejection . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Antibody mediated inhibition experiments suggest the involvement of CD3 / TCR and class 1 MHC molecules on the surface of CD4+ inducer T cells and the CD 2 , CD3 / TCR , CD 8 , and CD11a / CD18 molecules on the surface of CD8+ Ts during both the induction and the effector phase of Ts function . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Two other T cell surface proteins , CD 4 and CD 8 , which bind non polymorphic regions of class 2 and class 1 MHC molecules respectively , are also involved in these recognition events and play an integral role in thymic selection . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Cytotoxic T lymphocytes ( CTL ) expressing the CD 8 glycoprotein recognize peptide antigens presented by class 1 major histocompatibility complex ( MHC ) molecules . ^^^ This correlation and the absence of CD 8 polymorphism led to the hypothesis that CD 8 binds to a conserved site of class 1 MHC molecules . ^^^ Using a cell cell binding assay we previously demonstrated specific interaction between human class 1 MHC ( HLA A , B , C ) molecules and CD 8 . ^^^ Our results indicate that CD 8 binds to the alpha 3 domain of class 1 MHC molecules . . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Attempts to block the C 2 mediated cytolysis by a series of monoclonal antibodies showed that both the CD 3 T cell receptor complex and the CD 8 accessory molecule were required for OD T cell interaction and confirmed the lack of involvement of polymorphic MHC products as epitope presenting structures . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
The responses were resistant to natural killer ( NK ) cell depletion and were inhibited by monoclonal antibodies ( MoAbs ) to the T cell receptor , CD 8 surface antigens , and MHC class 1 antigens , suggesting a classical MHC class 1 restricted , virus specific CTL response . . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Monoclonal antibodies against CD 2 , CD 4 , CD11a , CD 18 , LFA 3 , ICAM 1 , class 1 and class 2 major histocompatibility complex ( MHC ) inhibited T cell proliferation in this response , whereas antibodies against CD 8 , CD11b , CD11c and CD 16 had no effect . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Thus , it appears that the interaction of the CD 4 and CD 8 accessory molecules on developing thymocytes with a ligand in the thymic environment ( probably MHC Ag ) is necessary for the positive selection of thymocytes into the appropriate T cell lineage . . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
It has been demonstrated , however , that a distinct CD 3 associated TCR is expressed on a small subset of T cells or immature thymocytes which fail to express either CD 4 or CD 8 ( refs 7 14 ) , the molecules associated with class 2 or class 1 MHC antigen recognition . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Next , we showed the non major histocompatibility complex ( MHC ) restricted cytolytic activity exhibited by these CD3+CD4 CD 8 cloned T cells and inhibition of the natural killer ( NK ) like activity by the anti CD 3 monoclonal antibody . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Since all patients receiving CD3+ , CD4+ , CD 8 marrow developed mild or moderate acute graft versus host disease ( GVHD ) , we conclude that CD4+ T cells are capable of initiating acute GVHD across non MHC barriers in man . . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
It was also found that none of the monoclonal antibodies tested ( including CD 8 and MHC class 1 antigen specific antibodies ) was able to block S1 / anti S 1 interactions . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Co expression of CD 4 and CD 8 molecules and de novo expression of MHC class 2 antigens on activated rat T cells . ^^^ Rat T cell populations were analysed for their surface expression of CD 4 , CD 8 and class 2 MHC antigens using monoclonal antibodies ( McAbs ) W3 / 25 , Ox 8 and Ox 6 in immunofluorescence and immunoenzyme cytochemistry . ^^^ Resting T cells expressed either CD 4 ( W3 / 25 ) or CD 8 ( Ox 8 ) molecules , but not both , and did not express class 2 MHC antigens . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
CTL effector functions are mediated primarily by cells carrying the CD 8 ( Ly 2 ) antigen ( CD8+ cells ) and are triggered by interactions of the T cell receptor with an antigenic complex , often termed ' self plus 10 ' , composed of viral determinants in association with class 1 molecules of the major histocompatibility complex ( MHC ) . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
We previously established TS clones derived from lepromatous leprosy patients which carry the CD 8 antigen and recognize antigen in the context of the major histocompatibility complex ( MHC ) class 2 molecules in vitro . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
These cells are categorized as the `` cortical type ' ' since they constitute most of the cortical cells ; they express both CD 4 and CD 8 antigens but only very low levels of MHC class 1 antigens . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
The 44 kd CD 8 associated protein was identified as the heavy chain of the class 1 major histocompatibility antigen by depletion in preclearing experiments with anti class 1 MHC antibody and by peptide mapping . ^^^ Further analyses indicated that the CD 8 class 1 MHC association is due , in part at least , to disulfide bonding , which may be susceptible to cleavage during processing of cell lysates . . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
The effector function of most MHC class 1 allospecific CTL is inhibited by anti CD 8 mAb . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
To determine the CD 4 or CD 8 phenotype of the Th lymphocyte which recognizes in vivo the MHC class 1 alloantigens , B 10 recombinant mice were treated with anti CD 8 or anti CD 4 mAb and immunized with lymphoid cells from donors differing in the K or D region of the MHC . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
In previous experiments we have introduced the T cell receptor alpha and beta chain genes from a CD 8 positive cytolytic T cell specific for the antigen fluorescein ( FL ) and the H 2D molecule of the major histocompatibility complex ( MHC ) into a CD 8 negative recipient cell . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
In all three models the phenotype changes in T cells associated with the early stages of activation i . e . , induction of receptors for interleukin 2 ( IL R ) , induction of MHC class 2 expression , and coexpression of CD 4 and CD 8 glycoproteins were not inhibited by CsA . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Cytotoxicity was inhibited by monoclonal antibodies directed against CD 3 , CD 8 , CD 2 , and lymphocyte function associated antigen 1 on the T cell , and against MHC class 1 proteins on the target cell . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
In this report we have analyzed cell cycle related fluctuations of both quantity and density of the T cell differentiation antigens , CD 3 ( T 3 ) , CD 4 ( T 4 ) and CD 8 ( T 8 ) , as well as the major histocompatibility complex ( MHC ) antigens on the cell surface of activated T cells . ^^^ Our results demonstrate that the T cell differentiation antigens , CD 3 , CD 4 and CD 8 , and class 1 MHC antigens are increased in density in the G 1 phase for all activated T cells tested . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
The CD 8 antigen is a marker for T lymphocyte subsets that is absent from helper T cells but expressed on cytotoxic T cells which recognize foreign determinants in association with class 1 major histocompatibility complex ( MHC ) antigens . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
The T cell differentiation antigen CD 8 has been implicated in the major histocompatibility complex ( MHC ) class 1 restricted specificity of CD 8 T cells . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Nonpolymorphic interactions between the T cell differentiation antigens CD 4 or CD 8 and major histocompatibility complex ( MHC ) encoded molecules have been postulated to participate in antigen recognition of MHC restricted T cells . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
We think that , in physiological T cell activation , cross linking of the T cell receptor to CD 8 or CD 4 is induced by their simultaneous binding to major histocompatibility complex ( MHC ) class 1 ( for CD 8 ) or MHC class 2 ( for CD 4 ) molecules on stimulator cells . ^^^ We consider the possibility that similar cross linking requirements may also exist during T cell repertoire selection in ontogeny , thus accounting for the strict coexpression of MHC class 1 and class 2 restricted T cell receptors with CD 8 and CD 4 molecules , respectively . . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
To explain this association , it has been proposed that these ' accessory ' molecules bind to monomorphic regions of the MHC proteins on the target cell , CD 4 to class 2 and CD 8 to class 1 products . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
The CD 8 antigen is a marker for those cytotoxic T cells that recognize antigen in the context of class 1 major histocompatibility antigens ( MHC ) and has now been identified in many species . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
In the present study we have reinvestigated the apparent contribution of CD 8 molecules to the overall avidity of interaction between CTL and their targets by using a recently developed system of major histocompatibility complex ( MHC ) class 1 restricted CTL clones that recognize defined peptide antigens . ^^^ We demonstrate that under conditions where the density of MHC , TcR and accessory molecules remains constant , the susceptibility of CD8+ CTL to inhibition of cytolysis by anti CD 8 antibodies is highly dependent upon the concentration and primary structure of the peptide antigen . ^^^ Although the precise role of the CD 8 molecule remains unknown , our results are compatible with models that suggest its contribution to the overall avidity of the CTL target cell interaction particularly in cases where the affinity between the TcR and antigen MHC is low . . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Mature T helper cells ( mostly CD4+ CD 8 ) recognize antigen in the context of class 2 MHC molecules , whereas cytotoxic T cells ( mostly CD 4 CD8+ ) recognize antigen in the context of class 1 MHC determinants . ^^^ As CD 4 CD8+ cells differ from the CD4+ CD 8 lineage ( in function , MHC restriction specificity and perhaps site of education ) we examined whether interactions with MHC determinants are also necessary for the development of class 1 specific T cells . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
This result provides strong evidence that deletion of potentially autoreactive T cells occurs at a CD4+8+ precursor stage , that the non clonally distributed accessory molecules ( CD 4 , CD 8 ) are significant participants in the self recognition process that leads to clonal elimination , and that thymic class 2 major histocompatibility complex ( MHC ) molecules can influence the repertoire of CD 4 8+ cells . . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
The membrane glycoproteins CD 4 ( L3T4 ) and CD 8 ( Lyt 2 ) are expressed on distinct populations of mature murine T lymphocytes , and are thought to be receptors for monomorphic determinants expressed on MHC class 2 and class 1 molecules , respectively . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Ag recognition of Lyt 2 ( CD 8 ) positive T lymphocytes requires the presentation by APC of a suitably processed Ag in association with MHC class 1 molecules . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
CD 4 and CD 8 molecules expressed on the surface of T cells bind to nonpolymorphic portions of class 2 and class 1 MHC molecules and assist the TCR in binding and possibly in signalling . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
The surface phenotypes ( CD 1 , CD 4 , CD 5 , CD 8 , SBU T 19 , MHC class 1 , MHC class 2 , and sIg ) of cells in blood , lymph nodes , and lymph were determined to examine simultaneously the distribution of lymphocyte subsets circulating in blood , afferent lymph , and efferent lymph of a peripheral lymph node . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Some of the major sheep antigens , including CD 5 , CD 4 , CD 8 , SBU T 19 , Pgp 1 , LCA and the MHC antigens , are described in detail . . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
In this study we present an immunocytochemical report on CNS tissue from an active case of MS , with an analysis of the distribution of CD 4 and CD 8 binding T cells and the expression of class 1 and 2 MHC determinants in plaques and white matter . ^^^ The preponderance of T cells of the cytotoxic / suppressor ( CD 8 ) phenotype in the CNS parenchyma was found in conjunction with widespread staining of class 1 MHC antigen , a prerequisite for activity of cytotoxic T cells . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
These data indicate that the capacity for MHC unrestricted tumoricidal activity and expression of NKH 1 and CD11b , but not CD 16 , are properties common to all or nearly all human peripheral blood derived T cell clones regardless of CD 4 or CD 8 phenotype . . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
The role of CD 8 as a receptor for class 1 MHC antigens was addressed by stimulation with a cell line expressing HLA DR antigens , but lacking the expression of HLA class 1 antigens ( Daudi ) . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
The ability of antibodies directed against Lyt 2 and L3T4 to block T cell function in a fashion determined by the class of MHC antigen recognized by the T cell further supports the hypotheses that Lyt 2 and L3T4 molecule in the mouse and Leu 2 and Leu 3 molecules in the human are involved in the interaction of the T cell with class specific determinants on MHC molecules . . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
The phenotype of these three clones was Leu 2 , Leu 3+ , Leu 4+ ; MHC restriction of their cytotoxic function was mapped to HLA DR by a panel of target cells as well as by inhibition of cytotoxicity with monoclonal antibodies . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
This study was directed at determining the major histocompatibility complex ( MHC ) antigens recognized by helper ( Leu 3 ) and suppressor cytotoxic ( Leu 2 ) T lymphocyte subsets in man . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Recently , our group reported following myoblast transplantations cellular infiltration of the host muscle by class 2 MHC cells , macrophages , and lymphocytes expressing CD 4 or CD 8 and IL 2 receptors . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
The CD 4 promoter is active in these cells ; they respond to antigens presented by MHC class 2 molecules ; they do not express CD 8 and they do not depend on MHC class 1 for their development . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
CD 4 and CD 8 are members of the immunoglobulin supergene family of proteins , and function as co receptors with the T cell receptor ( TCR ) in binding MHC class 2 or class 1 molecules , respectively . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
DNFB contact sensitivity ( CS ) in BALB / c and C3H / He mice : requirement for early occurring , early acting , antigen specific , CS initiating cells with an unusual phenotype ( Thy 1+ , CD5+ , CD 3 , CD 4 , CD 8 , sIg , B220+ , MHC class 2 , CD23+ , IL 2R , IL 3R+ , Mel 14 , Pgp 1+ , J11d+ , MAC 1+ , LFA 1+ , and Fc gamma RII+ ) . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Anti CD 54 and MHC 2 mAbs as well as a CD 8 alpha CD 40 ligand ( L ) soluble construct inhibited both the T dependent induction of Ig secretion , and B cell phenotypic changes . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
The morphological evaluation was in each case combined with immunofluorescence ( to detect immunoglobulins and complement fractions ) and immunohistochemistry with a wide panel of monoclonal antibodies for T cells ( CD 2 , CD 3 , CD 4 , CD 8 , gamma delta ) , B cells ( CD 20 , CD 22 ) , macrophages ( CD 68 , MAC 387 ) NK cells ( leu 7 , CD 16 ) , activation markers ( IL 2 R , Ki 67 , transferrin R ) , MHC antigens ( HLA ABC , HLA DR ) , adhesion molecules ( ICAM 1 , VCAM 1 , ELAM 1 , PADGEM , VLA 4 , LFA 1 alpha / beta ) , and growth factors ( EGF , TGF alpha , EGF R ) . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Although mature CD4+ T cells bear T cell receptors ( TCRs ) that recognize class 2 major histocompatibility complex ( MHC ) and mature CD8+ T cells bear TCRs that recognize class 1 MHC , it is possible that the initial commitment of an immature thymocyte to a CD 4 or CD 8 lineage is made without regard to the specificity of the TCR . ^^^ According to this model , CD4+ cells with class 1 TCR do not mature because the CD 8 coreceptor is required for class 1 MHC recognition and positive selection . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
These results show that Ly 2 ( CD 8 ) molecule plays an essential role in the interaction of CTL with MHC class 1 molecule only if T cell receptor has low affinity . . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
The abundant expression of the CD 8 accessory molecule by the IELs , in addition , indicates that these putative intestinal epithelial cell antigens are presented by MHC class 1 or 1 like molecules . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Analysis of T cell receptor 5 beta expression of the remaining DN T cells in beta 2m lpr mice showed a shift to a CD 4 like repertoire from a CD 8 like repertoire in control B6 / lpr mice , indicating that a small MHC class 2 selected DN population was unmasked in lpr mice lacking class 1 . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Collectively our data indicate that MHC class 1 molecules control the development of DN T cells but not autoantibody production in lpr / lpr mice and support the hypothesis that the majority of DN T cells may be derived from cells of the CD 8 lineage . . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
We have previously shown that coordinate binding of the H Y TCR and the CD 8 coreceptor to the restricting Db MHC class 1 molecule is required for the efficient positive selection of this TCR . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Antibodies used included : OX 6 antibody ( for MHC class 2 molecules , mainly expressed by dendritic cells ) : W3 / 25 and OX 8 antibody , for the demonstration of the rat equivalents of CD 5 and CD 8 , respectively : asialo GM 1 ganglioside antibody for the identification of natural killer ( NK ) cells and lymphokine activated killer ( LAK ) cells , and ED 2 antibody for labeling of macrophages . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Our data indicate that resistance to anti CD 8 mAb blocking correlates with strong binding affinity between sTCR and MHC / peptide complexes . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
TAPA 1 associates with several other membrane proteins on B and T cells , including MHC class 2 , CD 4 , and CD 8 , and it promotes Ca 2 ( + ) and LFA 1 independent homotypic aggregation when ligated directly or indirectly through CD 19 or CR 2 . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
This was shown in two different ways : first , CD 8 ( + ) cell mediated cytotoxic responses against L 1210 targets were associated with L 1210 specific and MHC restricted proliferative responses of syngeneic CD4+ cells and , second , CD4+ cells from mice that had received both ProT alpha and L 1210 tumor cells could enhance in vitro the otherwise weak , MHC restricted and L 1210 specific cytotoxicity of syngeneic CD8+ cells from mice that had received only L 1210 cells . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Most instances of MHC class 1 recognition and target cell killing by CD8+ CTL require the involvement of CD 8 . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
For serological analysis , monoclonal antibodies to CD 4 , CD 8 , TCR 1 , TCR 2 , MHC class 1 and class 2 antigens were used . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
After isolation of lymphocytes by gradient centrifugation , flow cytometric immunophenotyping was performed using CD 2 , CD 3 , CD 4 , CD 8 , CD11 / CD18 , CD 20 , CD 44 and MHC 2 monoclonal antibodies . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
The present results show that pretreating CTL with cis unsaturated , but not saturated , FA and low concentrations of PAO ( < 0 . 5 microM ) inhibit soluble anti TCR triggered binding of CD 8 to immobilized MHC class 1 , tyrosine phosphorylation of CTL substrates , PI turnover , and degranulation . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
The residues implicated in interaction with the co receptors CD 4 and CD 8 are not very well conserved in vertebrate evolution , and may reflect co evolution of the MHC molecules with their particular co receptors . . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Whilst the development of the CD 8 alpha / beta expressing 1 IEL is strictly contingent on major histocompatibility complex ( MHC ) class 1 surface expression , that of CD 8 alpha / alpha 1 IEL appears largely MHC class 1 independent . ^^^ We have used beta 2 microglobulin ( beta 2m ) / mutant mice lacking surface expressed MHC class 1 and TcR alpha / beta CD 8 alpha / beta+ 1 IEL to analyze the potential impact of MHC class 1 on regional activation of thymus independent 1 IEL . ^^^ The TcR gamma / delta 1 IEL and TcR alpha / beta CD 8 alpha / alpha+ 1 IEL from athymic nu / nu mice which express MHC class 1 , but lack TcR alpha / beta CD 8 alpha / beta+ 1 IEL , responded to TcR gamma / delta engagement as those from the beta 2m+ / controls . ^^^ The activation of TcR gamma / delta 1 IEL seems to be directly controlled by beta 2m / MHC class 1 expression and independent from TcR alpha / beta CD 8 beta+ 1 IEL . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Most studies of exogenous antigen processing have used soluble antigens , which are not efficiently presented by class 1 MHC molecules and do not elicit CD 8 T cell responses in vivo . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Cytotoxic T lymphocytes ( CTLs ) expressing the CD 8 surface marker recognize peptides in association with major histocompatibility complex ( MHC ) class 1 molecules . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
CD 4 , CD 8 and CD 3 were used to divide the populations into the conventional major categories , and the distribution of CD1a , CD 2 , CD 7 , CD 34 , CD 44 , class 1 MHC and class 2 MHC was then determined . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Competitive PCR quantification of CD 4 , CD 8 , ICAM 1 , VCAM 1 , and MHC class 2 mRNA in the central nervous system during development and resolution of experimental allergic encephalomyelitis . ^^^ We used competitive polymerase chain reaction to quantify messenger RNA for the lymphocyte antigens CD 4 and CD 8 , the adhesion molecules ICAM 1 and VCAM 1 , and the MHC class 2 1 A molecule in the spinal cords of SJL / J mice at multiple times during the development and resolution of experimental allergic encephalomyelitis ( EAE ) . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Unlike the findings with MHC class 1 specific TCR transgenic models , peripheral T cells in these mice include a substantial fraction of MHC class 2 specific ( 2B4+ ) T cells expressing CD 8 plus the transgene encoded CD 4 . ^^^ Together with the studies of others , these data support a stochastic model for CD 4 versus CD 8 lineage commitment of an MHC class 2 specific TCR during T cell development in the thymus . . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Additional studies examining the effect of CsA on thymic differentiation revealed that although 5 beta 8 . 5 is not normally clonally deleted , there was a pronounced shift in the expression of this determinant between CD 4 and CD 8 single positive thymocytes , suggesting that CsA may inhibit normal positive selection processes for MHC class 1 and class 2 reactive T cells . . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
The role of resident and newly recruited M phi responding to BCG in liver , spleen , lung and brain has been defined by means of antigen markers expressed by M phi ( F4 / 80 , 7 / 4 , CR 3 , macrosialin , sialoadhesin and scavenger receptor ) and / or T and B lymphoid cells ( MHC Class 2 , CD 4 , CD 8 , B 220 ) . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Here , we describe studies with knock out mice deficient in the TCR beta gene , lacking alpha / beta T cells ; in the TCR delta gene , lacking gamma / delta T cells ; in the beta 2m gene , lacking beta 2 microglobulin , and hence cell surface expressed MHC class 1 and functional CD 8 T cells ; and in the H 2I A beta gene , lacking cell surface expressed MHC class 2 and hence functional CD 4 T cells . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
The present study has utilized a novel coreceptor reexpression assay to identify lineage commitment in immature thymocytes and has found that the MHC recognition requirements for CD 4 commitment and CD 8 commitment fundamentally differ from one another . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
CD 8 downregulation started on day 2 after cell proliferation even in the absence of MHC class 2 expression . ^^^ The analysis of the 5 beta 14 TCR frequencies in CD4 / 8 subsets in normal and MHC deficient mice confirmed that CD 4 and CD 8 generation pathways are not symmetrical . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
These data demonstrate the importance of both MHC 1 and MHC 2 dependent immune mechanisms in acquired resistance to L . monocytogenes and point to the necessity of a coordinated interaction between CD 8 and CD 4 alpha beta T cells ( and probably gamma delta T cells ) in anti L . monocytogenes resistance . . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
These markers consist of T cell specific antigens ( CD 2 , CD 3 , CD 4 , and CD 8 ) , activated T cell antigens ( CD 25 and CD 71 ) , major histocompatibility complex ( MHC ) antigens ( class 1 and 2 ) , and adhesion molecules ( LFA 1 and ICAM 1 ) . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
The influence of allo class 2 MHC specific Th 2 cells on the generation of CD 4 and CD 8 cytotoxic T cells to associated class 1 and class 2 MHC alloantigen . ^^^ Because clinical transplants involve multiple class 1 and 2 MHC disparities we tested how the switch to Th 2 activity of tolerant mice would affect the generation of CD 4 and CD 8 cytotoxic T cells ( CTL ) against MHC alloantigens to which the mice were not tolerant . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Induction of a MHC class 1 restricted , CD 8 positive cytolytic T cell response by chimeric HIV 1 virus like particles in vivo : implications on HIV vaccine development . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Previous studies have shown that both MHC class 1 and class 2 molecules can stimulate CD4+CD8+ thymocytes to develop into TCRint cells with surface phenotypes , suggesting initial differentiation along either the CD 4 or CD 8 developmental pathways . ^^^ In accord with the view that these cells have undergone lineage commitment , we show here that CD4intTCRint cells generated by recognition of either MHC class show the same partial down regulation of CD 8 from the level on precursor double positive thymocytes as do almost mature CD4intTCRhigh cells positively selected only by MHC class 1 recognition . ^^^ These findings are consistent with the hypothesis that transitional phenotype thymocytes have begun but not completed positive selection , and that entry into the CD 4 vs CD 8 pathways is not uniquely determined by the specific coreceptor MHC molecule interaction involved in precursor thymocyte activation . . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
We have examined these questions by analyzing survival and differentiation of early ( CD4+CD8+TCRhi ) and later ( CD 4 CD8+TCRhi ) postselection stages of thymocytes from normal and bcl 2 transgenic mice expressing transgenic , class 1 MHC restricted TCR , upon intrathymic transfer into recipients that lacked ligands either for both the TCR and CD 8 coreceptor , or for the TCR only . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
The T lymphocyte co receptors of MHC glycoproteins CD 4 and CD 8 are known to be associated with the protein tyrosine kinase Lck via cysteine containing sequences in the cytoplasmic domains of CD 4 and CD 8 and in the N terminal domain of Lck . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
While no striking effects on 5 beta 8 . 2 and 8 . 5 usage were observed , a 3 to 4 fold over representation of 5 beta 10 in the CD 4 as compared with the CD 8 subset in all strains suggested a preference of 5 beta 10 for MHC class 2 products . ^^^ Finally , a dramatic overselection of 5 alpha 8+ CD 8 T cells by RT1f ( 14 % in RT1f versus 1 2 % in other haplotypes ) provides the most striking case yet for an intrinsic affinity of a TCR 5 segment for an MHC product . 5 alpha 8+ CD 8 T cells are not only overselected by RT1f in the thymus , but also during the alloreactive response of peripheral CD 8 T cells to RT1f . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
CD 4 and CD 8 interact with nonpolymorphic regions of class 2 and class 1 major histocompatibility complex ( MHC ) molecules , respectively , and these interactions result in increased intercellular adhesion and enhanced stimulation of T cells . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Thymocytes and class 1 major histocompatibility complex ( MHC ) restricted cytotoxic T lymphocytes express predominantly heterodimeric alpha / beta CD 8 . ^^^ By interacting with non polymorphic regions of MHC class 1 molecules CD 8 can mediate adhesion or by binding the same MHC molecules that interact with the T cell antigen receptor ( TCR ) function as coreceptor in TCR ligand binding and T cell activation . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
The actual requirement for CD 8 interaction with class 1 MHC molecules to promote the appearance of CD 4 CD8 T cells in lpr / lpr mice has also been suggested . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
The peptide mediated proliferation and cytolytic reactions were blocked by antibodies against CD4 / class 2 MHC and CD 8 molecules . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Some markers , including class 1 and class 2 MHC , CD 8 alpha and BP 1 , appeared to be integral components of the DC surface . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Species specific monoclonal antibodies suggest that PAECs directly present swine MHC antigens to human T cells and that human CD 4 and CD 8 molecules participate in this interaction . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Signaling properties of CD 8 have been ascribed to the alpha chain , which binds to both the MHC class 1 and to p56lck , respectively . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
The generation of the tumour specific CTL response could be blocked by monoclonal antibodies against all the molecules involved in the cognate interactions ( i . e . class 1 MHC , CD 8 , class 2 MHC , CD 4 and TCR ) but not by anti CD 2 or anti IgG . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Our results demonstrate that whereas expression of major histocompatibility complex ( MHC ) molecules , CD 45 , Mac 1 , LFA 1 , B cell markers , macrophage markers and some T cell markers ( as CD 2 , CD 4 and CD 8 ) did not undergo changes in culture , the level of expression of the adhesion molecules VLA 4 and ICAM 1 , and the T cell markers CD 5 , CD 25 and Thy 1 increased after 14 h incubation at 37 degrees C . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
The ectodomains of the T cell surface glycoproteins CD 4 and CD 8 bind to membrane proximal domains of MHC class 2 and class 1 molecules , respectively , while both cytoplasmic domains interact with the protein tyrosine kinase ( PTK ) p56lck ( lck ) through a shared cysteine containing motif . ^^^ Function of CD 4 and CD 8 requires their binding to the same MHC molecule as that recognized by the T cell antigen receptor ( TCR ) . ^^^ Studies using CD 4 transgenic mice indicate that development of single positive T cells involves stochastic downregulation of either CD 4 or CD 8 , coupled to activation of a cytotoxic or helper program , respectively , and subsequent selection based on the ability of the TCR and remaining co receptor to engage the same MHC molecule . . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Genetic analysis of four differing traits ( MHC haplotype , tumor response , CD 4 : CD 8 ratio , ev 17 ) in CB and CC congenic chickens . ^^^ Two closely related inbred lines of chickens , CB and CC , were compared for major histocompatibility complex ( MHC ) haplotype , response to tumors induced by Rous sarcoma virus and CD 4 : CD 8 ratio . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Major histocompatibility complex ( MHC ) class 1 deficient and MHC class 2 deficient mice lack functional CD 8 T cells and CD 4 T cells , respectively . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
CTL activity was susceptible to partial blockade with mAbs directed against CD 8 and MHC class 1 , suggesting a relatively low affinity Ag TCR interaction . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
In contrast to this non MHC restricted , CS initiating cell , the classical late acting CS effector T cell that is recruited locally is CD3+ , CD4+ , CD 8 , and is MHC restricted . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Thus , interaction of DP thymocytes with MHC class 2 leads to the immediate down regulation of CD 8 , which occurs simultaneously with an increase in TcR expression ; DPTcR ( lo ) HSA ( hi ) thymocytes mature into a CD4+CD8 ( lo ) TcR ( hi ) HSA ( hi ) intermediate population . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
We have characterized a prominent ( 15 20 % ) thymocyte population expressing CD 4 at a high and CD 8 at a low level ( CD4+8lo ) in mice transgenic for a T cell receptor ( TCR ) restricted by major histocompatibility complex ( MHC ) class 1 molecules . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
A state of tolerance to MHC mismatched allografts can be generated in rodents by treatment with CD 4 and CD 8 monoclonal antibodies ( mAb ) . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Activation of peripheral CD4+ cells has been reported to induce simultaneous expression of CD 8 and MHC class 2 molecules in rats . ^^^ However , our results using LEC rat CD4+ cells suggest that Con A induced signaling pathways for CD 8 and MHC class 2 expression can be separable . . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
A revised version of the model has been proposed whereby commitment of double positive thymocytes to either the CD 4 or CD 8 lineage requires two engagements with MHC molecules : the first , initiating the differentiation program , signals down regulation of one or the other coreceptor , regardless of the T cell receptor ' s specificity for MHC class 1 or 2 molecules ; the second , leading to terminal differentiation , screens the choice of coreceptor by permitting only those cells with matched receptors and coreceptors to proceed . ^^^ Here we explore the role of coreceptors in the two stages of positive selection by manipulating CD 8 expression in MHC class 2 deficient mice , crossing them with either CD 8 negative animals or animals carrying combinations of CD 8 alpha and CD 8 beta transgenes . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
The two clones ' cytotoxic activity , while inhibited by anti LFA 1 mAb , could not be abolished by mAbs to CD 3 , to class 1 and class 2 MHC molecules , and by mAbs to molecules involved in T cell function ( i . e . , CD 4 , CD 8 , CD 2 ) . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
The CD 4 and CD 8 co receptors , together with a compatible TCR alpha beta , bind class 2 and class 1 MHC , respectively , delivering a signal that allows T cell maturation to proceed along the appropriate co receptor lineage . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Flow cytometric analysis showed that BNY 2 cells were positive for CD 4 and CD 5 and were negative for CD 8 and RT 6 , indicating that these cells were phenotypically Th cells rather than cytotoxic T cells . mAbs against rat MHC class 2 Ags ( anti RT1 . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
These data demonstrate that : ( 1 ) chronic non A , non B , non hepatitis can be treated with interferon ; ( 2 ) interferon activates NK cells and enhances hepatocyte expression of Class 2 MHC antigens ; and ( 3 ) interferon also increases the number of CD 3 , CD 4 , and CD 8 cells found within the liver but these changes do not distinguish between responders and non responders . . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
However , by the mid stage of the cycle there was a significant and dramatic fall in CD2+ T cells and other lymphocytes expressing the CD 4 , CD 8 and CD 1 phenotypes , MHC class II+ cells were predominant throughout the endometrium . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
In conclusion , the interaction avidity of a single thymocyte , which is important for selective processes , might be affected by the expression levels of each interacting component : TCR , CD 4 or CD 8 and MHC molecule and self antigen . . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Anti major histocompatibility ( MHC ) class 1 and 2 and CD 4 and CD 8 monoclonal antibodies ( 50 micrograms / ml ) significantly inhibited CTL activity . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Effect of MHC class 1 and CD 8 cell deficiency on experimental autoimmune myasthenia gravis pathogenesis . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Mutant mouse strains lacking MHC class 1 ( beta 2 microglobulin null ) or class 2 ( A beta null ) expression fail to generate normal CD 8 or CD 4 T cell populations and provide model systems for reconstitution experiments . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Other antibodies are markers for MHC class 1 and class 2 molecules , for B cells , granulocytes , macrophages , T cell subsets distinct from those defined by CD 4 and CD 8 , and other sub populations of horse leucocytes that do not have obvious counterparts in humans , rodents , or other species . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
On both peripheral blood lymphocytes and thymocytes , PMA resulted in a rapid and transient down regulation of equine CD 4 expression , but had no such effect on the surface expression of equine CD 5 , CD 8 or major histocompatibility complex ( MHC ) class 1 and class 2 molecules . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Coculturing CD 8 cells with EC stimulates a sufficient level of endothelial class 2 MHC expression to subsequently support a CD 4 T cell proliferative response . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
During T cell activation , CD 4 and CD 8 form a ' bridge ' between the T cell receptor ( TCR ) and major histocompatibility complex ( MHC ) class 2 and class 1 molecules , respectively . ^^^ Although CD 4 and CD 8 appear to perform similar immune functions , they are structurally diverse suggesting that their mode of interaction with the TCR and MHC molecules may differ . ^^^ These data will be evaluated in light of our current understanding of CD 8 : MHC class 1 , and CD 4 : TCR interactions . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
If lineage commitment is initially independent of MHC specificity , expression of ectopic CD 8 in CD 4 cells should allow CD 4 cells with class 1 specific TCR to mature . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
While the results of these experiments are inconsistent with a model in which CD 4 versus CD 8 lineage is determined by an initial TCR / MHC / co receptor interaction , they also do not support a simple stochastic model of lineage commitment . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
The different roles of MHC class recognition in thymocyte CD 4 versus CD 8 lineage commitment and positive selection . ^^^ We suggest here that these and other recent data are most consistent with a model in which the TCR mediated decision to enter the CD 4 versus the CD 8 lineage is independent of the class of MHC molecule recognized and is distinct from positive selection . ^^^ This latter event appears to involve already lineage committed cells and to require a match of the MHC class specificity of the lineage defining , highly expressed CD 4 or CD 8 coreceptor and the TCR . . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
We suggest that a selective model in which two TCR MHC molecule engagements are required : the first induces a random down modulation of either CD 4 or CD 8 and some differentiation ; the second , involving the participation of the appropriate coreceptor , permits end stage differentiation . . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Using TCR transgenic mice , we present evidence that CD4+ CD8+ thymocytes are precommitted to either the CD 4 or CD 8 lineage , prior to positive selection and independently of TCR specificity for MHC . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Certain antigens which delineate clinical useful lymphocyte subsets such as CD 2 , CD 4 , CD 8 , CD 14 , CD 16 , CD 20 , and MHC class 2 are apparently well conserved since most human cell specific antibodies identified the homologous cell subset in monkeys . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Recent studies have provided compelling evidence that Hly also facilitates recognition of listerial antigens , in association with major histocompatibility complex ( MHC ) class 1 molecules , by CD 8 T lymphocytes . ^^^ Furthermore , BMM phi infected with M 3 , M 20 or SLCC 53 were as efficiently lysed as BMM phi infected with the Hly positive wild type strain EGD by MHC class 1 dependent CD 8 cytotoxic T lymphocytes . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Recent studies have shown that both CD 4 and CD 8 interact with conserved regions of MHC class 2 and class 1 , respectively . ^^^ These results directly demonstrate a functional role for these regions of MHC molecules and underscore the central role of both CD 4 and CD 8 in the effective initiation of a CTL response . . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Approximately 90 % of gamma / delta T cells from peripheral lymphoid organs have the same cell surface phenotype as thymus derived MHC class 1 restricted alpha / beta T cells , i . e . , they are CD 4 but express the CD 8 alpha / beta heterodimer together with CD 2 and CD 5 . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Three color analysis of CD 4 , CD 8 and MHC class 1 demonstrated that normal rat thymocytes contain CD 4 8 MHC class Ihi cells , which increased in number after GC treatment . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
There was no demonstrable alteration in the percentage of cells positive for CD 3 , CD 4 , CD 8 , CD 25 or CD 56 in peripheral blood or urine populations in six patients treated with intravesical IL 2 , or the pattern of MHC class 1 or 2 expression on tumour biopsies before and after treatment . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
A combination of mAb 1C11 , which binds to a cell surface glycoprotein on lymphoma cells , and of Abs to the differentiation markers CD 4 and CD 8 ( MHC co receptors ) , and CD 3 ( TCR complex ) was used to characterize the phenotypes of preneoplastic thymocytes and to place them within the scheme of normal T cell ontogeny . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
The role of cosignalling in the generation of MHC unrestricted cytotoxicity of T cells was studied with CD4+ and CD8+ sub populations highly purified ( > 98 % ) by immunomagnetic cell sorting using OKT 4 mab , Dynal anti CD 4 mab , OKT 8 mab , Dynal anti CD 8 mab , and OKT 3 mab . ^^^ Cytotoxicity was determined in 4 h cytotoxicity assays against K 562 tumor cells known to lack expression of MHC class 1 and class 2 antigens , thus avoiding interference with anti CD 4 or anti CD 8 mediated signalling . ^^^ In CD8+ cells , the first signal delivered by the sorting mab ( immobilized OKT 8 or Dynal anti CD 8 or OKT 3 ) only induced low MHC unrestricted cytotoxicity but committed the cells to develop largely enhanced cytolytic potential upon stimulation with a second ( IL 2 or RG ) or third ( OKT 3 , IL 2 , RG ) signal . ^^^ Dynal anti CD 4 mab was inefficient and OKT 3 , as sorting mab of CD4+ cells from CD 8 depleted PNAC , appeared to block subsequent OKT 4 induced generation of MHC unrestricted cytotoxicity by delivering a negative signal . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
The CD 8 and MHC Class 1 antigens played a role in emperipolesis . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
DC from both sources were isolated by digestion with collagenase , disruption of cellular complexes with a chelating agent , selection of light density cells , immunomagnetic bead depletion of other cell types ( without depletion with anti CD 4 or anti CD 8 ) and finally sorting for cells expressing high levels of class 2 MHC . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
The T cell composition of the thymus of 10 ray irradiated ( 3 . 5 Gy ) Wistar rat protected with WR 638 was analyzed by flow cytometry using monoclonal antibodies directed to the Thy 1 . 1 , CD 43 , CD 2 , CD 5 , CD 4 , CD 8 and class 1 and 2 MHC antigens . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
In addition MHC 2 was found to be present on a large proportion of both the mutually exclusive equine T lymphocyte subpopulations which express either the equine homologues of CD 4 , or CD 8 . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
We propose a selective model entailing two TCR MHC molecule engagements : the first provokes random down modulation of either CD 4 or CD 8 and a degree of differentiation ; the second , requiring participation of the appropriate coreceptor , permits end stage differentiation . . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
During thymic maturation , CD 4 CD8 TCR immature thymocytes differentiate through a CD4+CD8+TCRlo intermediate into two functionally distinct mature T cell subsets : helper T cells expressing CD 4 and a major histocompatibility complex ( MHC ) class 2 restricted T cell receptor ( TCR ) , and cytotoxic T cells expressing CD 8 and and MHC class 1 restricted TCR . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
The ability of donor CD 8 cells to facilitate engraftment could reflect the activity of a cytokine uniquely elaborated after recognition of an MHC class 1 disparity . ^^^ More likely , the graft enhancing effect of donor CD 8 cells may result from the generation of MHC class 1 specific or class 1 restricted cytotoxic T cells that recognize the host CD 4 and CD 8 cells responsible for causing rejection . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
CD 4 and CD 8 are expressed on mutually exclusive T cell populations that can recognize peptides bound to class 2 and class 1 MHC Ags , respectively . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
We propose a model whereby residues located on the CDR like loops of the CD 8 homodimer interact with the alpha 3 domain of MHC class 1 while amino acids on the side of the molecule containing the A and B beta strands contact the alpha 2 domain of class I . . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Con A treatment led to a significant increase in the percentage of cells bearing CD 8 , CD 6 , MHC Class 2 and J 5 , but it had no effect on the percentages of cells positive for the other T cell markers CD 5 , CD 4 , or CD 2 . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
No alterations in other cell phenotypes tested ( CD 4 , CD 8 , CD 2 , CD 6 , B cells , monocytes or MHC class 2 ) were observed , nor were there changes in lymphokine activated killer ( LAK ) , natural killer ( NK ) cell activity , or oxygen radical formation ( assessed by reduction of nitroblue tetrazolium ) . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Most of the neoplastic cells from five cattle with EBL appeared to be divided into four types , CD 2 , CD 4 , CD 8 , WC 1 , B2+ or B 2 , IgM+ or IgM , MHC II+ and strong TAA+ . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
A role of CD 8 in the veto effect was further implicated by rhesus MLR induced CML experiments in which pretreatment of normal responder cells with MAb to MHC class 1 , the natural ligand of CD 8 , blocked the suppressive activity of allogeneic BMC . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Lysis of AuTu ( A2+B44+ ) , PCI 13 ( A2+B44 ) and PCI 4B ( A 2 B44+ ) by the CTL was efficiently inhibited by monoclonal antibodies ( MAbs ) to CD 3 , CD 8 , TCR alpha / beta or the major histocompatibility complex ( MHC ) class 1 antigens . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
The number of mononuclear cells staining for CD 3 , CD 4 , CD 8 , 3G8 , and the number of mononuclear cells , liver cells , and bile duct cells staining for class 1 and 2 MHC antigens in the biopsies was determined . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Immunofluorescence staining showed further that the difference could not be accounted by a discrepancy in the expression of CD 8 alpha alpha or CD 8 alpha beta complexes or MHC class 1 molecules . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
The glycoprotein CD 8 is believed to play an important role in the maturation and function of MHC class 1 restricted T lymphocytes . ^^^ A consistent result was obtained from CD 8 alpha deficient mice bearing the `` tailless CD 8 alpha ' ' and the MHC class 1 restricted 2C TcR transgenes . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
In F 2 segregants , the low CD 8 phenotype mapped to the MHC . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Under physiological conditions , T cell activation by major histocompatibility complex ( MHC ) antigen complexes requires engagement of both the T cell receptor ( TcR ) and the CD 4 ( or CD 8 ) accessory molecules . ^^^ It has been shown , however , that ligation of CD 4 and CD 8 can also inhibit T cell activation in an MHC independent way . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Thus , the present study demonstrates that ( a ) activation of lck in CD4+CD8+ thymocytes regulates distribution and expression of TCR components ; ( b ) unlike CD 4 molecules , CD 8 molecules on CD4+CD8+ thymocytes can not efficiently activate lck despite their significantly greater surface expression ; and ( c ) the amount of lck associated with CD 4 in the CD4+CD8+ thymocytes is inversely related to the extent of CD 4 engagement by MHC class 2 molecules in the thymus . . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Cytotoxic T lymphocytes ( CTL ) are generally specific for class 1 MHC proteins plus antigen and express CD 8 co receptor molecules . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
It was also noted that Ialow neonatal airway DC expressed adult equivalent levels of class 1 MHC , which suggests differences in capacity to prime for CD 8 ( + ) dependent versus CD 4 ( + ) dependent immunity to inhaled pathogens , during the early postnatal period . . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
This article discusses the possibility that anergic T cells , with the participation of appropriate co stimulatory molecules on their membranes , stimulate CD 8 cells with an alpha / beta TCR specific for peptides of the TCR of the anergic cell itself processed and presented by class 1 MHC . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Peptide specific CTL precursors recognize the peptide on the class 1 MHC of the CD8+ MLR cells and then receive a negative signal via CD 8 on these cells . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
The CD 8 coreceptor interacts with MHC class 1 molecules through an acidic loop in the MHC alpha 3 domain . ^^^ In long term culture , HLA Cw 4 specific CTLs predominated , indicating that continuous expansion of allogeneic CTLs depends upon an efficient CD 8 MHC class 1 interaction . . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
MHC class 1 antigens are required for the action of CD 8 positive cytotoxic T cells . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
The effect of ammonium chloride and a commercial red cell lysing agent ( Erythrolyse ) on cell subset ( CD 4 , CD 8 , B cell common leucocyte antigen ) and activation ( p55IL 2R [ OX 39 ] , MHC class 2 ) antigen expression by freshly isolated and in vitro activated rat peripheral blood lymphocytes was compared . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Using monoclonal antibodies in immunohistochemistry , the distribution of the cells with the following surface antigens was studied in samples of proximal and distal small intestine of five 6 month old pigs : CD 2 , CD 4 ( helper / inducer T cells ) , CD 8 ( suppressor / cytotoxic T cells ) , accessory cell marker ( monocyte / granulocyte ) , MHC Class 2 ( DRw ) , and interleukin 2 ( IL 2 ) receptor . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Infiltrating cells mostly consisted of CD 4 and CD 8 T cells , and some proximal tubular cells presented MHC class 2 antigen . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
The presence or absence of class 2 MHC molecules does not appear to play a role in the non responsiveness to SEB , since CD 8 alpha+ beta IEL from class 2 deficient mice also failed to respond to stimulation with SEB . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
This T cell response was inhibited by mAbs directed against CD 4 or MHC class 2 molecules but not by mAbs specific for CD 8 or MHC class 1 molecules . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
By analyzing mice deficient MHC class 2 antigens alone or in combination with MHC class 1 antigens , no evidence was found for positive selection of 5 delta 4+ cells among CD 8 alpha + or CD 4 CD8 subpopulations of gamma delta T cell receptor positive cells . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Unexpectedly , a subpopulation of morphologically characteristic TIDC expressed both CD 8 and MHC 2 molecules . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
In suspension culture with alpha beta T cell receptor ( TcR ) transgenic thymocytes , these epithelial cells and dendritic cells ( pre pulsed with peptide cognate for the transgenic TcR ) caused down regulation of CD 4 , CD 8 , and TcR in an antigen dose dependent and MHC restricted manner . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
During this process , cells expressing T cell receptors ( TCRs ) reactive with self major histocompatibility complex ( MHC ) class 1 or 2 molecules are positively selected to the CD 8 or CD 4 lineage , respectively . ^^^ One population of CD4+CD8+ cells is positively selected by MHC class 1 molecules to the CD 8 lineage as expected , whereas the other CD4+CD8+ population rearranges endogenous TCR genes and is positively selected by MHC class 2 molecules to the CD 4 lineage . ^^^ These data support a version of the stochastic model in which CD4+CD8+ thymocytes are precommitted to the CD 4 or CD 8 lineage independently of TCR specificity for MHC and prior to positive selection . . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
To evaluate the role of CD 8 in positive and negative selection during thymic ontogeny , mice rendered CD 8 null by gene targeting were bred with three lines of transgenic mice expressing unique MHC class 1 restricted TcR . ^^^ In all three instances CD 8 was required for positive selection of MHC class 1 restricted transgenic T cells . ^^^ Surprisingly , there was a differential requirement for CD 8 expression in negative selection of MHC class 1 restricted thymocytes , depending on the antigen specificity of TcR . ^^^ These observations show that CD 8 is essential for positive selection but is differentially required for negative selection of MHC class 1 restricted T cells . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
The increase in CTL activity was mainly inhibited by anti MHC class 1 and CD 8 monoclonal antibodies ( 50 micrograms / mL ) , while it was partially inhibited by anti MHC class 2 or CD 4 antibodies in some patients . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Recently , a conserved site of DR beta 2 domain has been identified that involves CD 4 interaction that is analogous to MHC class 1 binding site for CD 8 molecule . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Most T lymphocytes expressed the CD 8 antigen ( OX8+ ) and hence were of the MHC class 1 restricted phenotype . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Sixteen of the resulting T lymphocyte cell lines ( from the S 19 and S19+IL 2 groups ) were tested through indirect immunofluorescence for expression of cell surface markers CD 2 , CD 4 , CD 6 , CD 8 , major histocompatibility complex ( MHC ) Class 2 molecules and a marker expressed on a subset of helper T lymphocytes ( Th ) as well as sIgM , CD 1 and a MHC Class II+ monocyte / macrophage marker . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
By analogy with T cell responses to conventional peptide antigens , it might be predicted that CD 4 ( MHC class 2 restricted ) cells would respond better to SE than CD 8 ( MHC class 1 restricted ) cells . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Because these mice lack cell surface MHC class 1 expression , they are deficient in T cells of the CD 8 subset ( class 1 restricted cytotoxic T cells ) . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Different effects of substitutions at residues 224 and 228 of MHC class 1 on the recognition of CD 8 . ^^^ This indicates that the substitution at residue 228 affects recognition of CD 8 dependent CTL but does not critically affect binding of CD 8 to MHC class 1 molecules , although residue 224 abrogates binding of CD 8 . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
The majority of human intestinal IEL express the TCR alpha beta , the CD 8 accessory molecule , and the CD45RO Ag , suggesting that they are MHC class 1 restricted memory T cells . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Experiments from many laboratories have provided concordant results leading to the consensus that CD 4 and CD 8 are functional analogues , providing similar supplementary signals to those generated through the TCR CD 3 complex on MHC class 2 and MHC class 1 restricted T cells , respectively . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Under the same conditions , cross linking other T cell surface determinants such as CD 4 , CD 8 , or class 1 MHC on preactivated T cells had no effect . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
CD 8 and beta 2 microglobulin free MHC class 1 molecules in T cell immunoregulation . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
None of the culture conditions examined induced MHC 2 expression on CD 4 and CD 8 T lymphocytes from BLV seronegative cows . ^^^ In contrast , SAC+IL 2 and PWM induced MHC 2 expression on CD 4 and CD 8 T lymphocytes from BLV infected PL cows , resulting in a significantly greater proportions of these lymphocyte subsets expressing this molecule compared to CD 4 and CD 8 T lymphocytes from control cows . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
IgV homologue domain to class 1 MHC binding was evaluated using a series of chimeric human CD 8 alpha : Fc polypeptides incorporating alternative CD 8 alpha extracellular domain components . ^^^ IgV homologue domain only ( dissociated from the 48 amino acid CD 8 alpha connecting peptide ) were shown to retain the capacity of the complete CD 8 alpha extracellular domain to bind to a recombinant soluble class 1 MHC alpha 3 domain unit or to intact class 1 MHC . ^^^ The specificity of the CD 8 alpha : class 1 MHC alpha 3 domain interaction was verified by mAb and soluble polypeptide blocking experiments . ^^^ GPI anchorage for this truncated CD 8 alpha polypeptide was verified , and its capacity to promote intercellular adhesion through class 1 MHC binding was shown in a cell : cell binding assay . ^^^ IgV homologue domain acts as an independent structural unit when dissociated from the CD 8 alpha connecting peptide , and in so doing retains class 1 MHC binding capacity . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
The epithelial cell recognition is not restricted by classical MHC class 1 or class 2 molecules , although the CD 8 alpha alpha participation in the recognition suggests the involvement of nonclassical MHC molecules . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Since CTL are major histocompatibility complex ( MHC ) class 1 restricted , we addressed the question of whether CD 8 / mice can reject MHC class 1 disparate allografts . ^^^ We therefore used the skin allograft model to answer our question and grafted CD 8 / mice with skins from allogeneic mice deficient in MHC class 2 or in MHC class 1 ( MHC 1 or MHC 2 disparate , respectively ) . ^^^ CD 8 / mice rejected MHC 1 disparate skin rapidly even if they were depleted of CD4+ cells in vivo ( and were thus deficient in CD4+ and CD8+ T cells ) . ^^^ Following MHC 1 , but not MHC 2 stimulation , allograft specific cytotoxic activity was detected in CD 8 / mice even after CD 4 depletion . ^^^ Therefore , MHC class 1 restricted CTL can be generated in CD 8 / mice following priming with MHC class 1 antigens in vivo . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Taking advantage of a potent MHC class 1 restricted response that allows the identification of antigen selected CD 8 T cells directly ex vivo , we characterized the antigen specific T cell repertoires that develop in individual mice by single cell PCR analysis . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
The data reviewed in this chapter suggest that the primary developmental function of the CD 4 and CD 8 coreceptors is to improve the efficacy by which a thymocyte recognizes peptide / MHC . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
The CD 8 phenotype of more than 70 % of the induced effector cells ( 97 % for cells from one cat ) was consistent with MHC class 1 restricted cytotoxicity . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
The data here clearly demonstrate that CD4low TCRint thymocytes do not belong to the CD 8 lineage maturation pathway , and suggest that these cells could represent a MHC class 2 restricted dead end subset . . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NKL cells express CD 2 , CD 6 , CD11a , CD 26 , CD 27 , CD 29 , CD 38 , CD 43 , CD 58 , CD 81 , CD 94 , CD 95 , class 2 MHC , and the C1 . 7 . 1 antigen , but do not express detectable levels of CD 3 , CD 4 , CD 5 , CD 8 , CD 14 , CD 19 , CD 20 , CD 28 , alpha / beta or gamma / delta T cell receptors on the cell surface . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Typical mature rat DCs were morphologically similar to their mouse and human counterparts and expressed major histocompatibility complex ( MHC ) class 2 ( common part determinant of Ia ) , OX 62 ( integrin molecule ) , OX 7 ( CD 90 ) , ICAM 1 ( CD 54 ) , and CTLA 4 counterreceptor , but were negative for OX 8 ( CD 8 ) , OX 19 ( CD 5 ) , W3 / 25 ( CD 4 ) , and ED 2 , a rat macrophage marker . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
These results indicate that the generation of CD 8 alpha alpha+ lymphocyte population of alpha beta and gamma delta IEL is not dependent , but that of CD 8 alpha beta+ lymphocyte population of alpha beta IEL is dependent on beta 2m and / or TAP 1 dependent MHC class 1 molecules , expressed by the controlling cells present in the anatomical site , where the development of IEL takes place . . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
The relatively high levels of MHC class 1 expression in H RS cells in EBV+ HD cases were accompanied by significantly higher numbers of activated cytotoxic T lymphocytes ( CTLs ) as shown by the presence of increased numbers of CD 8 and granzyme B+ lymphocytes . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
MHC class 2 specific T cells can develop in the CD 8 lineage when CD 4 is absent . ^^^ The generation of mature CD 4 T cells from CD4+CD8+ precursor thymocytes usually requires corecognition of class 2 MHC by a TCR and CD 4 , while the production of mature CD 8 T cells requires corecognition of class 1 MHC by a TCR and CD 8 . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
The analysis of transitional subsets in mutant mice for either beta 2 microglobulin or major histocompatibility complex ( MHC ) class 2 further revealed that lineage commitment to the CD 8 lineage requires a TCR MHC class 1 engagement , presumably at the immature double positive stage of thymic development , while CD 4 commitment does not require an MHC class 2 mediated signal , but rather occurs by default . ^^^ Using the addition of MHC class 1 or class 2 expressing cells or the addition of total thymocytes to purified sorted transitional precursors for the duration of the cultures in vitro , we identified an additional stage of differentiation for both CD 4 and CD 8 lineages that requires a positive selection signal . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
At the eighth week , the number of infiltrating cells reached a peak and consisted of T lymphocytes ( CD 4 , CD 8 ) and macrophages ( ED 1 , MHC class 2 , CD11b , and CD 18 ) . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Further analyses of the virus specific in vitro immune response revealed a major histocompatibility complex ( MHC ) class 2 restricted helper T lymphocyte reaction involving CD 4 but not CD 8 molecules as restriction elements . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Thymic precursors express both CD 8 and CD 4 , and it has been suggested that the intracellular signals generated by CD 8 or CD 4 binding to class 1 or 2 MHC , respectively , might influence the fate of uncommitted cells . ^^^ A hybrid protein consisting of the CD 8 extracellular and transmembrane domains and the cytoplasmic domain of CD 4 ( CD 884 ) should bind class 1 MHC but deliver a CD 4 intracellular signal . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
We produced a hybrid antibody ( HAb ) combining a mAb specific for a class 1 MHC molecule with a soluble CD 8 molecule . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Such CD 8 + T cells , isolated from tumor bearing mice after antibody directed IL 2 therapy , exerted a MHC class 1 restricted cytotoxicity against the same tumor in vitro . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
In experiments in which nontransgenic population are used as T cell inocula , we noted decreased CD 4 expansion when Class 2 MHC was blocked by mAb treatment in vivo , an CD 8 expansion failed to occur in Class 1 MHC deficient hosts providing evidence that T cell regeneration in thymic deficient hosts largely occurs via TCR MHC mediated selection of peripheral T cell populations . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Binding of viral antigens to major histocompatibility complex ( MHC ) class 1 molecules is a critical step in the activation process of CD 8 ( + ) cytotoxic T lymphocytes . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Adoptively transferred CD4+ lymphocytes from CD 8 / mice are sufficient to mediate the rejection of MHC class 2 or class 1 disparate skin grafts . ^^^ We showed that BALB / cJ ( H 2d ) CD 8 / mice could reject allogeneic skin from C57BL / 6J ( H 2b ) mice deficient in MHC class 1 or in MHC class 2 Ags . ^^^ To understand the role of CD4+ cells in this process , we isolated them from CD 8 / mice and transferred them to BALB / cJ nude mice that had been grafted with allogeneic skin ( H 2b ) from animals deficient in MHC class 1 or MHC class 2 . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Because some CD 8 ( + ) SP cells express F3Ag , the relationship between F3Ag down regulation and positive selection is less obvious in DP cells of mice carrying MHC class 1 restricted tg TCR . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Although it is generally accepted that CD4+ Th cells are essential for CTL priming with peptides , it is not clear how long sequences devoid of any CD 4 epitope , or strict CD 8 epitopic sequences too short to be presented in a MHC class 2 restricted fashion , can generate such responses . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
In this report we show that poor stimulation by GPI linked class 1 MHC molecules is not simply due to a failure to interact with CD 8 , but to a fundamental difference in the way T cells respond to GPI anchored class 1 molecules . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Evidence for this includes : 1 ) the accumulation of CD 4+ T cells within the lamina propria , 2 ) epithelial damage closely related to the increase of CD 8 TcR alpha beta IELs , and 3 ) abnormal class 2 MHC molecule expression on epithelial cells of colonic mucosa . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
These results demonstrate that in the absence of conventional MHC class 2 restricted CD4+ T lymphocytes , CD 8 priming persists and mediates partial protective immunity to T . gondii . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Since the physiologic ligands for MHC 1 molecules are TCR and the CD 8 molecules , our data may suggest that MHC 1 molecules are instrumental in cellular interactions between T cells . . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Immunoelectron microscopy on ultrathin cryosections showed that CD 8 DMB molecules accumulated in late endocytic compartments sharing characteristics with lysosomes , like MHC class 2 compartments in APCs . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
We have recently described an MHC class 1 restricted response characterized by an overwhelming expansion of CD 8 cells expressing a Vbeta 10 TCR , thus allowing the identification of antigen selected cells directly ex vivo . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Immunohistochemistry was performed with antibodies against the macrophage markers ED 1 , ED 2 , and OX 42 , the MHC class 2 antigen marker OX 6 , the T lymphocyte markers CD 4 , CD 5 , and CD 8 , and other markers , such as alpha smooth muscle actin , proliferating cell nuclear antigen , and von Willebrand factor . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
In dogs and cats , such cells were recently described ; they display a phenotype very similar to that of human LC ( CD 1 , CD 8 , CD11 / 18 , CD 45 and MHC 2 positive for canine LC , and CD 18 , CD 4 , panleukocyte antigen and MHC 2 positive for feline ones ) and in both species , Birbeck granules are observed . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Analyses of Listeria epitope generation and the specificity of protective CD 8 ( + ) T cells have suggested that future research should focus on secreted protein antigens in specific resistance to infection and have increased our understanding of Listeria antigens presented by MHC class l b molecules . . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
There was no significant difference in the expression of several surface markers ( CD 4 , CD 8 , MHC Class 1 , MHC Class 2 , lymphocyte function associated antigen ( LFA ) 1 and LFA 3 ) on monocyte derived macrophages ( MDM ) by 5 days post MVV infection . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Monoclonal antibodies ( mAbs ) to surface markers of bovine lymphocytes MHC 1 , MHC 2 , B cells , T cells ( CD 2 , CD 4 , CD 8 and gamma / delta ) and interleukin 2 ( IL 2 ) receptor were tested in the goat by flow cytometry and using immunohistochemical methods . ^^^ The percentage of positive cells obtained by flow cytometry and its compartmentalisation in different tissue sections showed that the mAbs against MHC 1 , MHC 2 , CD 2 , CD 4 , CD 8 , gamma / delta and IL 2 receptor recognised lymphocyte subpopulations similar to those present in the bovine . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
When the LPL flow cytometry data were compared with the flow cytometry data obtained from PBL in these same H . pylori infected piglets , leukocytes bearing the CD 8 marker predominated in LPL whereas leukocytes bearing the CD 4 reactive and MHC class 2 markers predominated in PBL . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Monoclonal antibodies against CD 4 ( OX 38 ) , CD 8 ( OX 8 ) , CD11b ( OX 42 ) , MHC class 1 ( OX 18 ) , monomorphic MHC class 2 ( OX 6 ) , and ED 1 and polyclonal antibodies against tyrosine hydroxylase ( TH ) were used for immunohistochemistry . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
To monitor immune responses , the following monoclonal antibodies were used : OX 38 against CD 4 , OX 18 against MHC class 1 ( MHCI ) , OX 8 against CD 8 , OX 6 against MHC class 2 ( MHCII ) , OX 42 against CD11b , R 73 against alpha and beta T cell receptor ( TcR ) , and ED 1 . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
MAbs to class 2 MHC ( OX 3 and OX 6 ) , CD 4 ( W3 / 25 ) , CD 8 ( OX 8 ) , and alpha beta TCR ( R 73 ) stimulated the adhesion via an LFA 1 dependent pathway , whereas an anti CD 45 mAb ( G3C5 ) stimulated the rosette formation independently of LFA 1 . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Eight genes ( Gamma actin , carcinoembryonic antigen , apolipoprotein A 2 , cardiac beta myosin heavy chain , laminin B 2 chain , MHC class 1 CD 8 alpha chain , c reactive protein , and retinoblastoma susceptibility protein ) containing large dinucleotide repeat units ( N > or = 15 ) , complete genomic structure information , and homologous gene sequences in a second species were selected . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Following intraocular maturation , grafts were analysed using antibodies against : major histocompatibility complex ( MHC ) class 1 , MHC class 2 ; rat antigens CD 4 , CD 8 , CD11b ; T cell receptor ; rat antigen ED 1 ; and glial fibrillary acidic protein . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
CD 25 and class 2 MHC molecule expression and the CD 4 : CD 8 ratio remained unchanged over the comparable period in the syngeneic LEW to LEW control model ( n = 5 , p > 0 . 3 for all comparisons ) . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
We previously reported that the 70 kDa heat shock cognate protein like molecule ( hsc 70 ) is expressed on the cell surface along with the neoplastic transformation of rat fibroblast and that this molecule is recognized by CD3+ , CD 4 , CD 8 , NKR P 1 , and TCR alphabeta T ( DNT ) killer cells in an MHC class 1 unrestricted fashion . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Subsequently , we showed that sorted subsets of predominantly CD 8 positive and CD 8 negative NK cells lysed a MHC class 1 positive tumor cell line at the same level . ^^^ This suggests that CD 8 is not likely to participate as a receptor for MHC class 1 in NK cell mediated lysis in a syngeneic rat model . . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
We conclude that self MHC 1 antigens induce tolerance in the CD 8 T cell compartment via negative selection when expressed exclusively by lymphoid cells . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Thus , the participation of CD 8 and CD 4 T cells as primary effectors of graft rejection depends on which class or classes of MHC antigens are constitutively expressed on graft parenchymal cells and are available for recognition . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Expression of activated Notch permits the development of mature CD 8 lineage thymocytes even in the absence of class 1 major histocompatability complex ( MHC ) proteins , ligands that are normally required for the development of these cells . ^^^ However , activated Notch is not sufficient to promote CD 8 cell development when both class 1 and class 2 MHC are absent . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
We propose that in MHC unrestricted recognition , a large number of MUC 1 epitopes is necessary to effectively engage the T cell receptor , and that in the presence of a low number of epitopes , engagement of the CD 8 co receptor by MHC class 1 molecules may be required for completing the signal through the T cell receptor . . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Mouse myeloma cell line secreting bifunctional fusion protein RM4 / IFN gamma [ corrected ] elicits antitumor CD 8 MHC class 1 restricted T cells that are cytolytic in vitro and tumoricidal in vivo . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Further , H 2K antigen expression is significantly more readily inducible on B 16 alpha res cells than on B 16 cells , consistent with enhanced CD 8 mediated killing due to increased MHC class 1 antigen expression . . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
No major aberration in cell surface carbohydrate expression was found and the expression of cell surface molecules including CD 43 , CD 4 , CD 8 , and MHC class 1 and 2 was normal . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
CD 8 can serve as a coreceptor in that it binds to the same MHC class 1 molecules as the TCR to facilitate efficient TCR signaling . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
After MV infection , murine cells expressing CD 46 were lysed by MHC class 1 restricted CD 8 CTLs specific for the ectodomain of H . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
How T cells emerge from the intrathymic differentiation process with selective expression of either CD 8 molecule or CD 4 molecule coordinated with the MHC class specificity of the TCR has been the subject of intense examination . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Frozen sections of eyelids and conjunctiva were immunostained with the following monoclonal antibody markers : W3 / 13 ( CD 3 ) , W3 / 25 ( CD 4 , macrophages ) , OX 8 ( CD 8 ) , MARD 3 ( B cells ) , ED 1 , ED 2 ( macro / monocytes ) , OX 6 ( class 2 MHC , Ia ) and OX 39 ( CD 25 , IL 2 receptor ) . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Furthermore , an unusually high proportion of gamma delta positive TILs express CD 4 or CD 8 molecules ( 17 . 2 and 36 . 8 % , respectively ) , indicating that they might recognise antigen presented within MHC 2 or 1 context . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
As a prelude to examining the effect of this limited MHC class 1 diversity on the tamarin CD 8 ( + ) T cell receptor ( TCR ) repertoire , we identified expressed tamarin TCR beta chain ( TCRB ) cDNAs by anchored and inverse polymerase chain reaction . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
CD 8 enhances formation of stable T cell receptor / MHC class 1 molecule complexes . ^^^ MHC / TCR recognition is followed by the generation of a signal to the T cell through a monomorphic multicomponent system that includes the CD 3 complex and accessory molecules such as CD 4 and CD 8 . ^^^ We report here that the affinity of CD 8 dimers for MHC class 1 molecules is independent of haplotype and peptide content , and that the affinity of the TCR for its specific ligand is enhanced through a reduced ' off ' rate in the presence of either CD8alpha alpha homo or CD8alpha beta heterodimers . ^^^ Moreover , CD 8 seems to help recognition of the specific MHC peptide complex either by guiding an energetically favourable docking of TCR onto MHC , or by inducing conformational changes in the MHC complex that can augment the TCR / MHC peptide interaction . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
To investigate whether the presence or absence of HLA A , B and C antigens in laryngeal carcinoma influences survival , a series of 60 primary laryngeal tumours treated surgically and normal tissues were evaluated in frozen sections for the expression of MHC class 1 antigens and tumour infiltrating leucocytes ( CD 3 , CD 4 , CD 8 , CD11b , CD 1 , CD 20 and CD 16 ) , using monoclonal antibodies and the APAAP , technique . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
In both instances , the typical MHC class 2 expression on keratinocytes and the presence of ED1+ macrophages were identical to the lesions in the primary donors , where both CD 4 and CD 8 T cells were present . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
In addition , the phenotype of the cells was measured using flow cytometry with antibodies against CD 4 , CD 8 , CD 5 , MHC class 2 , CD 25 , CD 14 and CD 19 . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
The aims of this study were to measure the immunosuppressive ability of castanospermine ( CAST ) in a rat heart allograft model , to measure its effect on membrane expression of adhesion molecules ( LFA 1 alpha , LFA 1 beta , ICAM 1 ) , class 1 and class 2 MHC antigens and on other T cell associated molecules ( CD 4 , CD 8 , CD 39 , CD 45 , W3 / 13 ) , to test its tolerogenic potential and its toxicity . ^^^ ICAM 1 expression on endothelial cells of the allograft vasculature , class 1 and class 2 MHC expression on lymphoid cells in the thymus , class 2 MHC expression on lymphoid cells in the allograft ; and CD 4 , CD 8 , CD 45 and W3 / 13 expression on lymphoid cells in some organs . ^^^ In conclusion , CAST is an immunosuppressive molecule which may work by downregulation of the ligand receptor adhesion molecule pair , LFA 1 alpha ICAM 1 although subtle downregulation of class 1 and 2 MHC , CD 4 and CD 8 molecules could also contribute to its immunosuppressive activity . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Immunohistological studies were also carried out using a two step alkaline phosphatase technique to explore the expression of tumour associated antigens ( TAAs ) ( E 7 , 19A211 , T 138 ) , major histocompatibility complex ( MHC ) molecules and lymphocyte subset infiltrates ( CD 3 , CD 4 , CD 8 ) in bladder biopsies before and 3 weeks after the completion of treatment in seven patients . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Immature CD4 / CD8 double positive ( DP ) thymocytes expressing self MHC restricted TCR are positively selected in response to TCR signals to survive and differentiate into functionally competent CD 4 or CD 8 single positive ( SP ) T cells . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Immune CD8+ T cell mediated lysis of infected hepatocytes was restricted by classical MHC class 1 ( H 2Kb ) molecules and was inhibited by the presence of either brefeldin A or mAb specific for CD 8 . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
The inflammatory foci contained large numbers of B 220 ( + ) and MHC Class 2 ( + ) cells and lesser numbers of CD 3 ( + ) , CD 4 ( + ) , and CD 8 ( + ) cells . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
This study confirms accepted notions ( B and T dependent zones ) and supplies complementary data about the repartition of CD 4 and CD 8 cells , gamma delta T cells , MHC ( Major Histocompatibility Complex ) 2 expression , and macrophages . . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Assays for the specific surface antigens CD 45 , CD 2 , CD 4 , CD 8 , CD 1 , MHC class 2 , and N 1 were employed to develop immunophenotypic profiles within the gated lymphocyte clusters from each TIL and PBL suspension . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
This has been well documented for major histocompatibility complex ( MHC ) class 2 restricted responses , and more recently using peptide loaded and protein pulsed DC for CD 8 responses following adoptive transfer in mice . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Major histocompatibility complex ( MHC ) class 2 knockout ( class 2 ) mice fail to generate CD4+ CD 8 T lymphocytes . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Interestingly , flow cytometry showed differences between CD 8 on alveolar macrophages and on T lymphocytes within the ligand binding domain for MHC class 1 . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Frozen sections from eyes enucleated at 0 , 1 , 5 , 13 , and 33 days post laser were examined for T cells ( R7 . 3 ) , CD 4 T cells ( W3 / 25 ) , activated CD 4 T cells ( OX 40 ) , CD 8 T cells ( OX 8 ) , B cells ( OX 33 ) , and macrophages ( OX 42 ) , MHC 2 antigen ( OX 6 ) , and E Selectin 1 , VCAM 1 , and ICAM 1 . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Our data show that nonionic triblock copolymers enhanced presentation of soluble ovalbumin ( OVA ) to the major histocompatibility complex ( MHC ) class 2 restricted CD 4 ( + ) T cells and MHC class 1 restricted CD 8 ( + ) T cells , respectively . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Proliferation of CD 8 clones by stimulation with F 344 APC was inhibited by the addition of anti rat class 1 MHC mAb but not of anti class 2 MHC mAbs . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Development of mature CD 4 and CD 8 single positive T cells requires a process known as positive selection , which depends on the specific recognition of self peptide MHC complexes on thymic stromal cells by immature CD4+CD8+ thymocytes . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Frozen sections of small bowel were studied with monoclonal antibodies anti CD 5 , CD 4 , CD 8 , CD 25 , macrophages , MHC 2 . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
In this report , we demonstrate stimulation of T cell receptor ( TCR ) transgenic CD 8 T cells by isolated major histocompatibility complex ( MHC ) class 1 H 2Ld complexes and antigenic peptide . ^^^ This is the first demonstration of CD 8 T cells activated by MHC and antigenic peptide in the absence of antigen priming . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Monoclonal antibodies against major histocompatibility complex ( MHC ) class 1 , class 2 , CD 4 , CD 8 , pan T cells , and macrophages were used for immunostaining brains from adrenalectomized ( ADX ) and shamoperated rats to investigate the potential involvement of the immune / inflammatory mechanisms in the neurodegeneration of hippocampus after ADX . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
In vitro T cell responses to H Y are controlled by MHC class 1 and 2 Ir genes , which respectively , restrict CD 8 and CD 4 T cells : These can be isolated as T cell clones in vitro . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Sections of lacrimal glands removed 21 59 days post inoculation were immunostained using antibodies against rabbit Class 1 and Class 2 MHC molecules , CD 4 , CD 8 , CD 18 , and rabbit thymic lymphocyte antigen ( RTLA ) . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
The absence of cytotoxic T lymphocyte activity and the failure of MHC class 1 restricted T cell receptor ( TCR ) transgenic thymocytes to mature in CD8alpha deficient mice suggest that CD 8 may be essential for CD 8 lineage commitment . ^^^ Interestingly , there was no enhanced selection of CD4+ T cells , implying that the TCR MHC interaction , even in the absence of CD 8 , provided instructive signaling for commitment to the CD 8 lineage . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
A flexible loop of the alpha 3 domain ( residues 223 229 ) is clamped between the complementarity determining region ( CDR ) like loops of the two CD 8 subunits in the classic manner of an antibody antigen interaction , precluding the binding of a second MHC molecule . ^^^ Although these shifts in alpha 3 may provide a synergistic modulation of affinity , the binding of CD 8 to MHC is clearly consistent with an avidity based contribution from CD 8 to TCR peptide MHC interactions . . ^^^ The homodimer CD8alpha ( alpha ) or the heterodimer alpha beta stabilizes the interaction of the T cell antigen receptor ( TCR ) with major histocompatibility complex ( MHC ) class I / peptide by binding to the class 1 molecule . ^^^ Here we report the crystal structure at 2 . 7 A resolution of a complex between CD8alpha ( alpha ) and the human MHC molecule HLA A 2 , which is associated with peptide . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
A panel of monoclonal antibodies that identified a monocytegranulocyte marker ( GMI ) ; the CD 4 , CD 8 , IgM , MHC Class 1 , MHC Class 2 and T 19 antigens , and the gamma delta ( gamma delta ) T cell receptor was used . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Thus , maintenance of CD 8 T cell memory still required TCR MHC class 1 interactions , but memory T cells may have a lower functional activation threshold that facilitates secondary responses . . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
To test this hypothesis , we used a T cell receptor transgenic mouse in which essentially all CD 8 ( + ) T cells are specific for a closely related set of self peptides presented on the MHC class 1 molecule Ld . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Intraocular infection with herpes simplex virus type 1 strain F ( HSV 1 ) induces bilateral retinitis , the expression of both MHC class 1 and 2 molecules and activation of CD 4 and CD 8 cells . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
BAL cells were harvested and stained with antibodies to the equine homologues of CD 5 , CD 4 , CD 8 , CD 44 , MHC 1 , MHC 2 and to equine IgG . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
The lysis of porcine targets by the human CD8+ cytotoxic T lymphocyte clones was inhibited by monoclonal antibodies against SLA class 1 antigens and human CD 8 , which indicates that human CD8+ T cells recognize porcine MHC class 1 molecules . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
It has been demonstrated by several investigators that beta 2m / knockout mice are deficient in the expression of MHC Class 1 molecules but can nevertheless generate CD 8 ( + ) allospecific cytotoxic T cells following vigorous in vivo priming . ^^^ When splenocytes from naive unprimed beta 2m / mice were provided exogenous cytokines in MHC Class 1 disparate primary MLC , allospecific cytolytic effectors were generated . beta 2m / MHC Class 1 allospecific CTL that were CD3+ and Thy1 . 2+ were otherwise heterogeneous in phenotype , including CD8+ , CD4+ , CD 8 CD4 , TCR alpha beta+ , and TCR gamma delta+ T cells . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
The molecular interactions between the CD 8 co receptor dependent N 15 and N 26 T cell receptors ( TCRs ) and their common ligand , the vesicular stomatitis virus octapeptide ( VSV 8 ) bound to H 2Kb , were studied to define the docking orientation ( s ) of MHC class 1 restricted TCRs during immune recognition . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Amino acids in the alpha 2 and alpha 3 domains responsible for the binding of human CD 8 to MHC class 1 were largely conserved in the porcine genes , but several critical residues were altered . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Differentiation of CD 4 ( high ) CD 8 ( low ) coreceptor skewed thymocytes into mature CD 8 single positive cells independent of MHC class 1 recognition . ^^^ Mice transgenic ( tg ) for the major histocompatibility complex ( MHC ) class 1 restricted P 14 T cell receptor ( TCR ) , on the H 2bm13 background , generate a large number of CD 4 ( hi ) CD 8 ( lo ) CRS thymocytes . ^^^ Loss of CD 4 was independent of thymic stroma , up regulation of CD 8 to full levels was dependent on thymic stroma but independent of MHC haplotype . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
We have previously characterized two different CD 8 T cell responses , which are restricted by the same mouse major histocompatibility complex ( MHC ) class 1 molecule , H 2 Kd . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
CD 8 cytotoxic T cell clone rapidly transfers autoimmune diabetes in very young NOD and MHC class 1 compatible scid mice . ^^^ Thus , optimally activated islet specific CD 8 T cell clones are able to rapidly transfer diabetes to NOD and MHC class 1 compatible scid mice when a large enough number is administered at 7 days of age . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
We found that BCL XL expression promoted accumulation of CD 8 single positive thymocytes even in MHC class 2 restricted TCR transgenic mice . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
TCR 5 alpha elements are expressed preferentially in CD 4 or CD 8 subsets in a manner that is largely independent of MHC haplotype . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Here we report the generation of MHC class 1 restricted CD 8 ( + ) CTLs that surprisingly require target cell TAP deficiency for efficient recognition . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
A challenge for subunit vaccines whose goal is to elicit CD 8 ( + ) cytotoxic T lymphocytes ( CTLs ) is to deliver the antigen to the cytosol of the living cell , where it can be processed for presentation by major histocompatibility complex ( MHC ) class 1 molecules . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
In rats expressing the f allele of the rat MHC ( RT1f ) , CD 8 T cells utilizing the 5 alpha 8 . 2 segment are 10 fold overselected during thymic development , resulting in 5 alpha 8 . 2 expression by 14 % of mature CD 8 T cells as compared to 1 2 % in MHC congenic strains . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
CD 4 and CD 8 are thought to function as coreceptors by binding to the cognate major histocompatibility complex ( MHC ) molecules recognized by the T cell antigen receptor ( TCR ) and initiating the signal transduction cascade . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Mice immunized with heat shock proteins ( hsps ) isolated from mouse tumor cells ( donor cells ) produce CD 8 cytotoxic T lymphocytes ( CTL ) that recognize donor cell peptides in association with the major histocompatibility complex ( MHC ) class 1 proteins of the responding mouse . ^^^ Because large protein fragments or whole proteins serving as fusion partners can be cleaved into short peptides in the MHC class 1 processing pathway , hsp fusion proteins of the type described here are promising candidates for vaccines aimed at eliciting CD 8 CTL in populations of MHC disparate individuals . . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
ACD3S induced cytotoxicity was not inhibited by anti CD 4 , CD 8 and MHC class 1 MAbs , but was markedly reduced in the presence of MAb against CD 18 . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
The activation of CD 8 T cells was studied using transgenic mice expressing an MHC class 1 restricted OVA specific TCR ( OT 1 ) . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
During development , progenitor thymocytes differentiate into either CD 4 or CD 8 T cells , and this fate decision depends on the specificity of the T cell antigen receptor ( TCR ) for MHC class 2 or class 1 molecules . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
However , the role of CD 8 in the MHC independent tumoricidal activity of anti CD 3 activated killer T ( AK T ) cells has not been investigated . ^^^ The inhibition of MHC unrestricted cytotoxicity by anti CD 8 alpha mAb can not be attributed to interference with an adhesion like function of CD 8 towards class 1 MHC molecules on the target cells because anti CD 8 alpha mAb ( 1 ) had equal inhibitory effects on the cytolysis of tumor target cells regardless of their relative level of class 1 MHC molecule expression and ( 2 ) did not interfere with the formation of conjugates between AK T cells and class 1 MHC bearing P 815 tumor cells . ^^^ Taken together , these data suggest that CD 8 is able to affect the tumoricidal activity of MHC unrestricted AK T cells independent of class 1 MHC molecules on the target cell . . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Avidin biotin enhanced immunocytochemistry was used to detect expression of canine CD 3 , CD 4 , CD 8 and MHC Class 2 antigens . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
The CL 1 cells were positive for CD 45 and MHC class 2 and negative for CD 4 , CD 5 , CD 8 , Thy 1 and B cell specific antigen and surface immunoglobulin . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Kidneys were examined morphologically and immunohistologically using monoclonal antibodies ( mAbs ) against macrophages ( ED 1 ) , T cells and their subsets ( CD 5 , CD 4 , CD 8 ) , MHC class 2 expression ( OX 3 ) and adhesion molecules ( ICAM 1 and LFA 1alpha ) . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Rejection of Tg beta gal skin by a beta gal specific CD 8 cytotoxic T lymphocyte ( CTL ) effector mechanism should require presentation by donor and / or recipient LC of MHC class 1 restricted peptides of exogenous beta gal shed by keratinocytes . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
These results demonstrate that ASP 1 and ASP 2 are targets of in vivo generated CTLs and that this CTL response induced by T . cruzi infection is parasite and peptide specific , MHC restricted , and CD 8 dependent . . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
The PICC and the affected hair follicles were characterized during spontaneously developed or induced hair cycle stages , using antibodies against MHC Class 2 , F4 / 80 , ER MP 23 , NLDC 145 , CD 4 , CD 8 , gammadeltaTCR , IL 1 receptor , and ICAM 1 . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
This dichotomy as a result of antigen compartmentalization suggests that bacterial antigens are presented by multiple MHC class 1 pathways to prime CD 8 T cells , but only the endogenous pathway provides target antigens for CD 8 T cell mediated protective immunity . . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
To address this issue we used tetramers of MHC class 1 molecules containing viral peptides to directly visualize antigen specific CD 8 T cells during acute LCMV infection of mice . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
CD 8 molecules function as co receptors on cytotoxic T lymphocytes ( CTLs ) , interacting with a nonpolymorphic region of the major histocompatibility complex ( MHC ) class 1 a 3 domain on antigen presenting cells . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Phenotypic maturity , as measured by upregulated expression of TCR , class 1 MHC , and Qa 2 , and downregulated expression of heat stable antigen ( HSA ) on CD 8 SP cells in FP THY grafts , was similar to that in host thymi of euthymic control mice . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
We have , however , previously identified and partially characterized a novel functional population of CD8+ , but anti CD 8 resistant , MHC class 1 allospecific CTL . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Direct binding of the MHC class 1 molecule H 2Ld to CD 8 : interaction with the amino terminus of a mature cell surface protein . ^^^ Although the activation of T cells is controlled by the interaction of the TCR with MHC / peptide complexes , the degree and extent of the activation is influenced by the binding in parallel of the CD 8 coreceptor with MHC 1 . ^^^ In the course of quantitative evaluation of the binding of purified MHC 1 to engineered CD 8 , we observed that peptide deficient H 2Ld ( MHC 1 ) molecules bound with moderate affinity ( Kd = 7 . 96 10 10 ( 7 ) M ) , but in the presence of H 2Ld binding peptides , no interaction was observed . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
MHC class 1 restricted lysis of human oligodendrocytes by myelin basic protein peptide specific CD 8 T lymphocytes . ^^^ Previous studies indicate that it is possible to generate MHC class 1 restricted myelin peptide specific cytotoxic CD 8 T cells , and that human adult OLs express MHC class 1 molecules in vitro . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
The cells also expressed marginal levels of MHC class 1 , CD 14 , CD 40 , and CD 80 , but lacked detectable MHC class 2 , CD 4 , CD 8 , CD45R , and CD 102 molecules . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
CD 8 is a T cell surface glycoprotein that participates in recognition of peptide / MHC class 1 molecules by binding to their alpha 3 domains . ^^^ We have used the techniques of co capping and confocal microscopy to ask whether we can detect an association between CD 8 and the TCR independently of their binding to MHC class 1 molecules . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
One , 4 and 8 weeks later , brains were analyzed using antibodies against MHC class 1 ( OX 18 ) , MHC class 2 ( OX 6 ) , CD 4 ( OX 38 ) , CD 8 ( OX 8 ) , OX 42 , ED 1 , NF , GFAP , AChE and TH . ^^^ At week 1 , in ventro frontal areas of cortex , CD 8 positive cells and MHC 1 positive astroglial fibers were detected . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Comparison of the roles of CD 8 alpha alpha and CD 8 alpha beta in interaction with MHC class 1 . ^^^ The CD 8 molecule is expressed either as an alpha / alpha homodimer or an alpha / beta heterodimer on thymocytes and cytotoxic T cells , and functions as a coreceptor in concert with TCR for binding the MHC class I / peptide complex . ^^^ The abilities of different forms of CD 8 to adhere to MHC class 1 were measured with a cell cell binding assay . ^^^ To understand the role of CD8beta in mediating CD8 / MHC class 1 interaction , we examined whether cell surface CD8alpha / beta heterodimer promotes better cell cell adhesion with MHC class 1 than the CD8alpha / alpha homodimer . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
We have investigated the role of MHC class 1 products and CD 8 T cells in regulating Ab responses using beta 2 microglobulin deficient ( beta2m / ) mice . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Mice , but not rats , therefore may use CD 8 alpha ' as a `` dominant negative ' ' coreceptor chain to attenuate the CD 8 signal , thereby facilitating MHC class 2 recognition through the higher amount of p56lck delivered , and rats may use a different mechanism for MHC class distinction during positive selection . . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Current data suggest that CD8beta mediated response enhancement proceeds through mechanisms similar to those mediated by CD8alpha , i . e . , interacting with MHC class 1 and stabilizing CD 8 associated Lck activity . ^^^ In this study we present evidence that the extracellular portion of CD8beta is capable of independent interaction with MHC class I / beta2m dimers in the absence of CD8alpha . ^^^ In addition , CD8beta may enhance interaction with MHC class I / beta2m when associated with CD8alpha . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
However , analysis of cell surface marker expression by FACS revealed a surface phenotype similar to that of pre B cells ( MHC I+ , MHC II+ , B7 . 2+ , sIgM , sIgG , kappa , B 220 , CD 5 , Thy1 . 2 , TcR , CD 3 , CD 4 , CD 8 , Mac 1 , 33D1 ) . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Ovine cutaneous squamous cell carcinoma : immunohistochemical expression of CD 3 , CD 4 , CD 8 and MHC class 2 antigens in the associated inflammatory infiltrate . ^^^ The immunohistochemical expression of CD 3 , CD 4 , CD 8 and MHC class 2 antigens in the cellular inflammatory infiltrate associated with early and advanced ovine squamous cell carcinomas ( OSCC ) , as well as actinic keratosis was analyzed . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Small synthetic peptide mimics of the CD 8 DE loop were shown to effectively block CD 8 binding to major histocompatibility complex ( MHC ) class 1 molecules and possess significant inhibitory activity on in vitro CD 8 ( + ) T cell function . ^^^ These results suggested that the DE loop region of the CD 8 protein is an important functional epitope mediating CD 8 MHC class 1 interaction and the activation of CD 8 ( + ) T cells , a finding that is consistent with the recently reported crystal structure of the CD 8 MHC class 1 complex . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
All CTL lines were TCR alphabeta , CD 8 positive cells , EBV specific , and MHC class 1 restricted . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
It is also clear , however , that T cell receptor ( TCR ) preferences for major histocompatibility complex ( MHC ) class 1 versus class 2 binding will strongly influence an individual clone ' s skewing to the CD 4 or CD 8 subset . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Serial frozen sections of grafts and controls were stained by immunoperoxidase for rat MAC 1 , class 2 MHC , CD 2 , CD 4 , CD 8 , B cells , VLA 4 , LFA 1 , L selectin , ICAM 1 , and VCAM 1 . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Cytotoxicity inhibition studies using MoAbs revealed that the cytotoxicity operated in MHC class 1 , CD 8 and alphabeta T cell receptor dependent manners . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Eight monoclonal antibodies ( specific for CD 3 , CD 2 , CD 4 , CD 8 , WC 1 , cell surface IgM , cell surface IgG and MHC class 2 molecules ) and an avidin / biotin complex method on frozen sections were used . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
To address this issue , we examined the T cell receptor ( TCR ) repertoire of virus specific CD 8 T cells in the mouse model of infection with lymphocytic choriomeningitis virus ( LCMV ) using three approaches : ( a ) in vivo quantitative TCR beta chain 5 segment and complementarity determining region 3 ( CDR 3 ) length repertoire analysis by spectratyping ( immunoscope ) ; ( b ) identification of LCMV specific CD 8 T cells with MHC class 1 tetramers containing viral peptide and costaining with TCR Vbeta specific antibodies ; and ( c ) functional TCR fingerprinting based on recognition of variant peptides . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Suppression of SIV replication by unstimulated CD 8 ( + ) T cells required direct contact and was major histocompatibility complex ( MHC ) restricted . ^^^ However , CD 3 stimulated CD 8 ( + ) T cells produced soluble factors that inhibited SIV replication in an MHC unrestricted fashion as much as 30 fold . ^^^ Our results indicate that inhibition of SIV replication by CD 8 ( + ) T cells from animals immunized with live attenuated SIV strains involves both MHC restricted and unrestricted mechanisms and that MHC unrestricted inhibition of SIV replication is due principally to soluble factors other than RANTES , MIP 1alpha , and MIP 1beta . . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Contact hypersensitivity in MHC class 2 deficient mice depends on CD 8 T lymphocytes primed by immunostimulating Langerhans cells . ^^^ Key words : CD 8 lymphocytes / MHC class 2 deficiency . . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Antigen specific receptors ( TCR ) on CD 8 T lymphocytes form relatively short lived complexes with their natural ligands : peptides in association with major histocompatibility complex ( MHC ) class 1 molecules , which consist of a polymorphic heavy chain and a conserved light chain , beta 2 microglobulin ( beta 2 M ) . ^^^ To produce soluble MHC peptide complexes in a form that would bind more stably and could be used to identify , count , and isolate CD 8 T cells having the appropriate TCR , we prepared multimeric MHC peptide complexes . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
The interaction of CD 8 with MHC class 1 resembles that between antibody and antigen in the use of loops from the CD 8 structure . ^^^ The interaction is of very low affinity and buries equivalent surface area to that between the TCR and MHC class 1 but while the TCR / MHC interface shows poor surface shape complementarity the match in the conservative interaction between MHC and CD 8 is precise . . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
The classical polymorphic MHC molecules of class 1 or class 2 bind peptides derived from the processed cytosolic or endosomal antigens and export them to the cell surface for presentation to the T cell receptors ( TcR ) of CD 8 or CD 4 T lymphocytes , respectively . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
In Egr 1 / TCR H Y double transgenic mice , efficient positive selection of H Y CD 8 ( + ) T cells occurred , even in mice on either a nonselecting H 2 ( d ) background or a beta 2 microglobulin ( beta2m ) deficient background in which the expression of class 1 MHC heavy chains is extremely low ; no positive selection was observed on a Kb / Db / beta2m / background where class 1 MHC expression is entirely absent . ^^^ Similarly , when the Egr 1 transgene was introduced into a class 2 MHC restricted TCR transgenic mouse line , Egr 1 / TCR double transgenic mice revealed increased numbers of CD 4 ( + ) T cells selected by class 2 MHC , as well as significant numbers of CD 8 ( + ) T cells selected by class 1 MHC ( for which the transgenic TCR might have weak affinity ) . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
The monoclonal antibody panel included RT 1 ( a ) ( ACI , MHC 1 ) combined with CD 2 , CD 4 , CD 8 , CD 16 , and CD 25 or RT 1 ( a , c ) ( bone marrow chimeras ) . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Perforin and MHC class 2 deficient mice ( with functional CD 8 ( + ) T cells and perforin molecules and an H 2 ( b ) haplotype ) had comparable demyelination and genotype , however , only the latter showed severe clinical disease . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Most of the infiltrating cells in the clusters were positive for CD 8 , HAM 56 or MHC class 2 antigen ; few cells were positive for CD 56 . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
CD 8 T cell differentiation usually requires corecognition of major histocompatibility complex ( MHC ) class 1 by the T cell receptor ( TCR ) and CD 8 , whereas CD 4 T cells differentiate as a consequence of MHC class 2 recognition by the TCR and CD 4 . ^^^ These results are in line with recent observations that antagonistic signals direct differentiation into the CD 8 lineage , regardless of MHC specificity . . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
This study compares class 1 versus class 2 major histocompatibility complex ( MHC ) restricted ovalbumin specific T cell antigen receptor ( TCR ) transgenic mice to demonstrate that after in vivo activation with antigen the emergence of CD 4 ( ) CD 8 ( ) B 220 ( + ) T cells occurs more frequently from a CD 8 ( + ) precursor than from CD 4 ( + ) T cells . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Vaccination can elicit CD 8 ( + ) cytotoxic T lymphocytes ( CTL ) that recognize peptides presented by class 1 MHC molecules . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Reverse transcription PCR ( RT PCR ) , competitive RT PCR , and Northern blot demonstrated that the expression of MHC class 2 group genes [ MHC class 2 , MHC class 2 associated invariant chain ( 2 ) , CD 4 and IgM ( B cell marker ) ] , MHC class 1 group genes ( MHC class 1 and CD 8 ) , B 7 1 ( costimulator on dendritic cells ) , and CD 28 ( receptor to B 7 on T cells ) in the pyloric mucosa was elevated by MNNG in both rat strains but was elevated to a 4 7 fold greater extent in Buffalo rats than in ACI rats . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
The ability to prevent rejection was reduced by the absence of CD 28 , because as many as 1 . 0 10 10 ( 7 ) CD 28 ( / ) CD 8 ( + ) cells were needed to prevent rejection of major histocompatibility complex ( MHC ) class 1 incompatible marrow in sublethally irradiated ( 550 cGy ) bm 1 recipients , whereas 8 . 0 10 10 ( 5 ) CD 28 ( + / + ) CD 8 ( + ) T cells were sufficient to produce a similar effect , indicating that CD 28 on donor CD 8 ( + ) cells helps to eliminate host immunity . ^^^ Two million CD 8 ( + ) CD 28 ( / ) or CD 28 ( + / + ) T cells were transplanted into sublethally irradiated ( 750 cGy ) , MHC class 1 incompatible ( B 6 10 bm 1 ) F 1 recipients . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
B / mice controlled an acute LCMV infection with the same kinetics and efficiency as B cell competent ( B+ / + ) mice via virus specific , major histocompatibility complex ( MHC ) class 1 restricted CD 8 ( + ) cytotoxic T lymphocytes ( CTL ) . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Cytotoxic activity of the CTL was inhibited by monoclonal antibodies against CD 3 , CD 8 , and MHC class 1 molecules . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
The inflammatory cells in the lesional tissue consist mainly of T lymphocytes , with a high CD 4 : CD 8 ratio , and major histocompatibility complex ( MHC ) class 2 expressing antigen presenting cells . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Pancreata and grafts were harvested at pre scheduled time points before onset of diabetes and at onset of diabetes , and stained for insulin , MHC class 1 , MHC class 2 , alphabeta TCR , CD 4 , CD 8 or ED 1 . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
DNA vaccination was evaluated with the experimental murine model of Trypanosoma cruzi infection as a means to induce antiparasite protective immunity , and the trypomastigote surface antigen 1 ( TSA 1 ) , a target of anti T . cruzi antibody and major histocompatibility complex ( MHC ) class 1 restricted CD 8 ( + ) cytotoxic T lymphocyte ( CTL ) responses , was used as the model antigen . ^^^ In H 2 ( d ) mice , inoculation with either of the two TSA 1 expressing vectors effectively generated antiparasite antibodies and primed CTLs that lysed T . cruzi infected cells in an antigen specific , MHC class 1 restricted , and CD 8 ( + ) T cell dependent manner . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Retroviral mediated expression of an MHC class 1 restricted T cell receptor in the CD 8 T cell compartment of bone marrow reconstituted mice . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
This killing activity was almost completely abrogated by antibodies to CD 8 or MHC class 1 , but not to CD 4 . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
CD 8 expression allows T cell signaling by monomeric peptide MHC complexes . ^^^ This response is not observed in CD 8 T cells and when either the CD 8 : MHC or CD 8 : Lck interactions are prevented . ^^^ This demonstrates that an intact CD 8 coreceptor is necessary for effective TCR signaling in response to monomeric peptide MHC molecules . ^^^ We propose that this heterodimerization of TCR and CD 8 by peptide MHC corresponds to the physiological event normally involved during antigen specific signal transduction . . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
In both species , coreceptor function apparently involves the participation of CD 8 dimer in a bidentate attachment to an MHC class 1 molecule in conjunction with a T cell receptor without discernable conformational alteration of the peptide or MHC antigen presenting platform . . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Specific CD 8 ( + ) CTL recognition of melanoma requires expression of MHC class 1 molecules as well as melanoma associated peptide epitopes . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Suppression of MHC class 1 expression is thought to allow tumor cells to escape immune surveillance mediated by CD 8 ( + ) CTLs . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Even though CD 8 cells could efficiently suppress HIV replication through both major histocompatibility complex ( MHC ) restricted cytotoxic killing of infected cells , particularly during primary infection , and HIV suppressing soluble factors , there is evidence that tissue infiltrating CD 8 lymphocytes mediate injury in several organs of HIV infected subjects . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
DX5+ cells did not express CD 8 , CD45R or MHC class 2 antigens . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
CONCLUSIONS : These data suggest that MHC antigens , CD 8 positive T cells , and perforin expressing cells contribute to intestinal graft rejection . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
At the CD 4 ( + ) CD 8 ( + ) ( double positive , DP ) stage , intermediate CD 5 levels are maintained by low affinity TCR major histocompatibility complex ( MHC ) interactions , and CD 5 surface expression is proportional to both the surface level and signaling capacity of the TCR . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Our results argue in favor of a selective model of thymocyte commitment , demonstrating a fundamentally symmetrical process : engagement of either class of major histocompatibility complex ( MHC ) molecule by a differentiating CD 4 ( + ) CD 8 ( + ) cell can give rise to T cell antigen receptor ( TCR ) hi thymocytes of either lineage . ^^^ Key findings include ( a ) direct demonstration of a substantial number of CD 4 committed , receptor / coreceptor mismatched cells in MHC class 2 deficient mice , a critical prediction of the selective model ; ( b ) highly efficient rescue of such `` mismatched ' ' intermediates by forced expression of CD 8 in a TCR transgenic line , and an explanation of why previous experiments of this nature were less successful a major past criticism of the selective model ; ( c ) direct demonstration of an analogous , though smaller , population of CD 8 committed mismatched intermediates in class 1 deficient animals . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Interactions of the T cell coreceptors , CD 4 and CD 8 , with MHC molecules participate in regulating thymocyte development and T lymphocyte activation and differentiation to memory T cells . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Populations of T cell receptor ( TCR ) alphabeta+ CD 4 ( + ) CD 54 ( + ) cells localized to gastric tissue of immunized C57BL / 6 wild type and MHC class 1 knockout mice , but TCRalphabeta+ CD 8 ( + ) cells predominated in the gastric tissue of immunized MHC class 2 deficient mice . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
The allografts were harvested and processed for morphometry and for immunohistochemical staining of MHC class 2 antigens , macrophages , CD 4 and CD 8 T lymphocytes , interferon gamma ( IFN gamma ) , and IFN gamma receptor . ^^^ RESULTS : With E 2 treatment , we observed that inducible MHC class 2 antigen expression is abolished in the media of the vascular allograft ; the expression of IFN gamma and IFN gamma receptor is unaffected ; and macrophage infiltration of the vascular allograft is inhibited significantly ( P < 0 . 01 ) , whereas the CD 4 and CD 8 T lymphocytes are not significantly ( P=0 . 07 ) suppressed . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
The response to intracerebroventricular administration of interferon ( IFN ) gamma was examined in the adult Wistar rat brain : major histocompatibility complex ( MHC ) antigens class 1 and 2 , CD 8 and CD 4 antigens , and the macrophage / microglia antigen OX 42 were analyzed in respect to saline injected cases over 1 week . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Phenotypic characterization of the tetramer binding CD 8 ( + ) lymph node T lymphocytes of the infected monkeys demonstrated a high level of expression of the activation associated adhesion molecules CD11a and CD49d , the Fas molecule CD 95 , and MHC class 2 DR . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
To re examine this issue we quantitated antigen specific CD 8 T cells during acute LCMV infection of mice using three sensitive techniques : ( 1 ) intracellular cytokine production , ( 2 ) single cell ELISPOT and ( 3 ) direct visualization of antigen specific CD 8 T cells by staining with MHC class 1 tetramers + peptide . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
By promoting DC maturation and presentation on both MHC class 1 and 2 molecules , ICs should efficiently sensitize DCs for priming of both CD 4 ( + ) helper and CD 8 ( + ) cytotoxic T lymphocytes in vivo . . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
The observation that some antigens that are normally presented by MHC class 2 and seen by CD 4 ( + ) T cells can be presented by MHC class 1 and stimulate CD 8 ( + ) T cells increases the possibility for such interactions . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
A CD 8 ( + ) cytolytic T lymphocyte ( CTL ) response to antigen presenting cells generally requires intracellular delivery or synthesis of antigens in order to access the major histocompatibility complex ( MHC ) class 1 processing and presentation pathway . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Recipient groups included mice with an acquired deficiency in CD 8 or CD 4 T cells caused by treatment with depleting mAb or mice genetically deficient in CD 8 or CD 4 T cells as a result of disruption of the genes encoding major histocompatibility complex ( MHC ) class 1 , MHC class 2 , CD 8 , or CD 4 . ^^^ In contrast , mice genetically deficient in either CD 8 T cells ( MHC class 1 or CD 8 knockouts ) or CD 4 T cells ( MHC class 2 or CD 4 knockouts ) rejected intestinal allografts promptly . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
BACKGROUND & AIMS : The intestinal epithelial compartment is populated by CD 8 ( + ) alpha beta and gamma delta intraepithelial lymphocytes ( IELs ) , which monitor the integrity of the epithelial barrier . alpha beta IELs are activated by peptide antigens presented by class 1 major histocompatibility complex ( MHC ) molecules , but it is unclear how gamma delta IELs are activated . ^^^ CONCLUSIONS : These results suggest that CD 8 ( + ) TCR gamma delta IELs do not require class 1 MHC for development but support the notion that antigens presented by class 1 MHC molecules are involved in the peripheral expansion and differentiation of this subset . . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
We have re investigated the roles of CD 4 and CD 8 T cell subsets in skin graft rejection across a single class 1 MHC disparity . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Activated T cells treated with sub lethal levels of BPD MA and light exhibited lower CD 25 levels , a temporary block in cell cycle transition , but unaltered expression of MHC Class 1 , CD 3 , CD 4 , CD 8 , CD 45 , CD 54 , CD 71 , CD 122 ( IL 2R beta chain ) or TCR beta chain antigens 24 h afterward . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
The T cell coreceptors CD 4 and CD 8 enhance T cell responses to TCR signals by participating in complexes containing TCR , coreceptor , and MHC molecules . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
In further experiments , hybrid constructs made of the extracellular portion of the murine CD 8 alpha chain and of the TM and the intracytoplasmic domains of H 2Kk class 1 MHC molecule were transfected into Jurkat T cells . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Recent studies of CD 8 ( + ) T cell responses against intracellular bacteria have provided insights into the relevance of the exogenous and endogenous MHC class 1 presentation pathways during the priming and effector stages . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Most importantly , our current study demonstrates that this MHC class 1 restricted IE 1 epitope of MCMV is efficiently presented to CD 8 ( + ) T cell clones in vivo and further strengthens the possibility of the potential use of CD 8 ( + ) T cell clones as immunotherapeutic tools against cytomegalovirus induced disease . . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
We have previously reported that CHS to dinitrofluorobenzene in mice is mediated by major histocompatibility complex ( MHC ) class 1 restricted CD 8 ( + ) T cells . ^^^ This was confirmed by in vitro studies showing that the hapten specific CTL activity was exclusively mediated by MHC class 1 restricted CD 8 ( + ) T cells which could use either the perforin or the Fas / FasL pathway for their lytic activity . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
This ' cytoplasmic ' pathogen is controlled by CD 8 T cells through MHC class 1 antigen presentation . ^^^ Such constructs are capable of introducing antigens into the MHC class 2 and MHC class 1 pathway , resulting in stimulation of both CD 4 and CD 8 T cells . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
T cell receptor and coreceptor CD 8 alphaalpha bind peptide MHC independently and with distinct kinetics . ^^^ The T cell surface glycoprotein CD 8 enhances T cell antigen recognition by binding to MHC class 1 molecules . ^^^ We show that human CD 8 alphaalpha binds to the MHC class 1 molecule HLA A 2 with an extremely low affinity ( Kd approximately 0 . 2 mM at 37 degrees C ) and with kinetics that are between 2 and 3 orders of magnitude faster than reported for T cell receptor / peptide MHC interactions . ^^^ Furthermore , CD 8 alphaalpha had no detectable effect on a T cell receptor ( TCR ) binding to the same peptide MHC class 1 complex . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
In conclusion , the MHC class I+ , 2 islet allograft paradoxically leads to a change in the donor reactive CD 4 T cell subset and not in the CD 8 subset . ^^^ We hypothesize that the tolerant state is not due to direct class 1 alloantigen presentation to CD 8 T cells but , rather , occurs via the indirect pathway of donor Ag presentation to CD 4 T cells in the context of host MHC class 2 molecules . . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
More specifically , the system is based on CD 8 ( + ) T cells selected in an environment with subnormal levels of MHC class 1 in the absence of beta 2 microglobulin . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Upon resumption of feed intake by the fourth d postweaning , villus height and crypt depth , CD 8 ( + ) T cell numbers , MHC class 1 RNA expression and local expression of stromelysin returned to Day 0 values . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Major histocompatibility complex ( MHC ) class 1 molecules present antigenic peptides to CD 8 T cells . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Tumor antigens presented by major histocompatibility complex ( MHC ) class 1 molecules and recognized by CD 8 ( + ) cytotoxic T lymphocytes ( CTLs ) may generate an efficient antitumor immune response after appropriate immunization . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Binding of a specific peptide ( s ) from a viral protein to major histocompatibility complex ( MHC ) class 1 molecules is a critical step in the activation of CD 8 ( + ) cytotoxic T lymphocytes ( CTLs ) . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
The CD 8 co receptor is important in the differentiation and selection of class 1 MHC restricted T cells during thymic development , and in the activation of mature T lymphocytes in response to antigen . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
The mRNA induction for the beta chemokines macrophage inflammatory protein 1alpha , macrophage inflammatory protein 1beta , and RANTES preceded the expression of markers for lymphocytes [ cluster of differentiation ( CD ) 4 , CD 8 ] , microglia / macrophages ( MHC 1 ) , and natural killer cells in the infarct area . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
In vitro , RSV has been shown to induce expression of several proteins on the respiratory epithelial cell , including RSV proteins , ICAM 1 , and MHC class 1 , that can potentially interact with CD 8 ( + ) T cells in initiating apoptosis of the target cell . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
A ) sequences and current models of TCR MHC peptide interaction suggests that this reduction in frequency of Tcrb V8 . 4a CD 8 cells may be a consequence of differential selection of Tcrb V8 . 2l versus Tcrb V8 . 4a TCR by differential binding of CDR2beta to highly conserved areas of C terminal parts of the alpha helices of class 1 MHC molecules . . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Recent technical breakthroughs in generating soluble MHC class 1 peptide tetramers now allow the direct visualization of virus specific CD 8 T cells after infection in vivo . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Misselected CD 8 cells that express T cell receptors ( TCRs ) that do not recognize class 1 major histocompatibility complex ( MHC ) protein can emerge from thymic selection . ^^^ Thus , inhibition of a death signal through continued TCR CD 8 coengagement of MHC molecules is a key checkpoint for the continued survival of correctly selected T cells . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
A panel of specific antibodies against CD 3 , CD 4 , CD 5 , CD 8 , MHC 1 and 2 was used in single and two colour flow cytometry to define T cell subpopulations in bronchoalveolar lavage fluid of horses affected with chronic obstructive pulmonary disease and of healthy controls . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Bone loss after oral infection was decreased in mice deficient in major histocompatibility complex ( MHC ) class 2 responsive CD 4 ( + ) T cells , but no change in bone loss was observed in mice deficient in MHC class 1 responsive CD 8 ( + ) T cells or NK 1 ( + ) T cells . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
In vitro , targets expressing syngeneic MHC were readily lysed by activated CD 8 ( + ) T cells from deletion deficient mice . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
The number of CD 8 SFC exceeded the number of memory cells detected in limiting dilution assays by > 1 log 10 , whereas a correlation was found between the frequency of effector cells detected by both ELISPOT and MHC class 1 peptide tetramer assays . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Therefore , the model system chosen here employed epitope specific MHC class 2 restricted CD 4 ( + ) T cells and MHC class 1 restricted CD 8 ( + ) T cells produced from previously immunized BALB / c mice . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
The choice between CD 4 and CD 8 T cell fates is also controlled by MHC recognition during positive selection , implying that recognition of class 1 or 2 MHC might regulate Notch signaling . ^^^ Possible models for the regulation of Notch by MHC recognition during CD 4 versus CD 8 lineage determination are discussed . . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
To test for a possible antigen presentation function of these macrophages , we used as an in vitro model the antigen beta galactosidase for which a dominant major histocompatibility complex ( MHC ) class 1 Ld restricted peptide epitope is known to be recognized by specific CD 8 cytotoxic T lymphocytes ( CTL ) . ^^^ Thus , Sn+ macrophages , which are significantly increased in the liver after ADI , may process tumor derived proteins via the MHC class 1 pathway as well as via the MHC class 2 pathway , as shown previously , and present respective peptide epitopes to CD 8 as well as to CD 4 immune T cells , respectively . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Furthermore , the peripheral T cell receptor ( TCR ) repertoire in these graft derived MHC class 2 expressing CD 4 ( + ) and in the CD 8 ( + ) T cell fractions was found to be restricted on the basis of TCR complementarity determining region 3 ( CDR 3 ) size profiles . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
A small fraction of rat 13 day old fetal liver ( r13FL ) cells , which weakly expressed the leukocyte common antigen CD 45 , constituted a homogeneous Thy 1 ( hi ) , CD 71 ( ) , CD 44 ( + ) , MHC class I+ , CD 43 ( + ) cell subpopulation negative for CD45RC , CD 3 , TCRalphabeta , TCRgammadelta , CD 2 , CD 5 , CD 4 , CD 8 , CD 25 , CD 28 , NKR P1a and sIg . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Regression was dependent on CD 8 T cells , and recurrent tumors were resistant to CTL , had substantially reduced expression of epitope mRNA , but retained the gp 160 gene , MHC , and processing apparatus . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Immune surveillance by CD 8 T cells requires that peptides derived from intracellular proteins be presented by MHC class 1 molecules on the target cell surface . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Only vaccinated mice receiving the tumor specific ch14 . 18 IL 2 fusion protein revealed a reactivation of CD 8 ( + ) T cells and subsequent MHC class 1 restricted tumor target cell lysis in vitro . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
To bypass the CD 4 MHC class 2 interaction requirements for positive selection and activation , we used mice that are doubly transgenic for CD 8 and for the MHC class 1 restricted TCR F 5 . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
We have proposed that molecules at the T cell / antigen presenting cell ( APC ) interface segregate according to size , with small `` accessory ' ' molecules ( e . g . , CD 2 , CD 4 , CD 8 , CD 28 , and CD 154 ) contributing to the formation of a close contact zone , within which the TCR engages peptide MHC , and from which large molecules are excluded ( Davis , S . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Major histocompatibility complex ( MHC ) class Ib molecules have been implicated in CD 8 ( + ) T cell mediated defenses against intracellular bacterial infection , but the relative importance of MHC class Ib restricted T cells in antimicrobial immunity is unknown . ^^^ This first direct characterization of an MHC class Ib restricted T cell response indicates that CD 8 ( + ) T cells responding to L . monocytogenes infection can be divided into two groups : H 2 M3 restricted responses , which provide rapid and quantitatively substantial effector function during primary infections but contribute relatively little to memory responses , and MHC class Ia restricted responses , which expand later during primary infection but form memory T cells that respond rapidly and dramatically in response to subsequent infections by the same pathogen . . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
We identified C CW triads of a similar configuration in other members of the IgSF such as T cell receptor ( TCR ) , major histocompatibility complex antigens ( MHC ) , cell surface antigens CD 4 and CD 8 , and cell adhesion molecules . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Three of four monoclonal antibodies ( mAbs 1 1C4 , L 243 , and 9 . 3F10 ) directed against disparate epitopes of human major histocompatibility complex ( MHC ) class 2 , as well as a mAb with specificity for CD 4 ( OKT 4 ) , inhibited the expansion of gammadelta T cells in response to Daudi cell stimulators . mAbs with a specificity for CD 74 and CD 8 were non inhibitory . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Furthermore , this difference is strictly correlated with the MHC haplotype and is the result of a reduction in the absolute number of CD 8 T cells in Brown Norway rats . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
In contrast to control mice , IL 3 treated Jak 3 ( / ) mice challenged with the allogeneic major histocompatibility complex ( MHC ) class 1 bearing tumor P 815 developed a specific CD 8 dependent cytotoxic T lymphocyte ( CTL ) response . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
In particular , the cytotoxic activities of NK cells and CD 8 ( + ) cytotoxic T lymphocytes ( CTL ) reactive against self MHC class 1 were assessed using a variety of gene targeted mice . ^^^ P ( o ) . beta2micro ( o ) ) enabled the determination that perforin was responsible for the cytotoxic activity of NK cells and CD 8 ( + ) CTL reactive against self MHC class 1 . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Current studies are focused on T cell receptor ( TCR ) / major histocompatibility complex ( MHC ) and costimulatory signals that activate human CD 4 and CD 8 T cells . . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Using intrathymic transfer , we show that the immature CD 8 ( lo ) CD 4 ( hi ) cells could complete their intrathymic maturation and populate the peripheral lymphoid organs in the absence of MHC class 2 ( and class 1 ) molecules . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Peripheral CD 8 ( + ) T cells mainly use CD8alpha / beta , and their development is mainly dependent on the major histocompatibility complex ( MHC ) class 1 proteins K ( b ) and D ( b ) in H 2 ( b ) mice . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Although the presence of all CD 8 ( + ) TCR alpha / beta ( 1 ) IELs is dependent on beta 2 microglobulin molecules , the nature of the major histocompatibility complex ( MHC ) class 1 molecules recognized by the CD8alpha / alpha and the CD8alpha / beta ( 1 ) subsets has remained elusive . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Positive selection of thymocytes induced by gene transfer : MHC class 2 mediated selection of CD 8 lineage cells . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Combining CD 5 null , MHC deficient and lineage specific reporter animals , we have investigated the influence of CD 5 on positive selection and the choice of CD 4 versus CD 8 lineage commitment on broad populations of thymocytes . ^^^ In mice lacking MHC class 2 molecules , however , increased numbers of transitional , class 1 selected CD4+ CD 8 ( int ) CD 3 ( hi ) cells were positively selected in the absence of CD 5 . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
CGP 53716 did not affect airway wall inflammatory cell proliferation , the number of graft infiltrating CD 4 ( + ) or CD 8 ( + ) T cells , ED 3 ( + ) macrophages , or the level of immune activation determined as IL 2R and MHC class 2 expression . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Cutting edge : TCR alpha beta+ CD 8 alpha alpha+ T cells are found in intestinal intraepithelial lymphocytes of mice that lack classical MHC class 1 molecules . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Respiratory challenge with the murine gammaherpesvirus 68 ( gammaHV 68 ) results in productive infection of the lung , the establishment of latency in B lymphocytes and other cell types , transient splenomegaly , and prolonged clonal expansion of activated CD 8 ( + ) CD62L ( lo ) T cells , particularly a Vbeta 4 ( + ) CD 8 ( + ) population that is found in mice with different major histocompatibility complex ( MHC ) haplotypes . ^^^ Apparently B cell CD 4 ( + ) T cell interactions play a part in the gammaHV 68 induction of both splenomegaly and non MHC restricted Vbeta 4 ( + ) CD 8 ( + ) T cell expansion . . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Here we studied these parameters in a long term CD 8 ( + ) T cell line using a tetrameric MHC reagent and the fluorescent dye CFSE . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
This capacity of ACT was recently exploited for in vivo delivery of single viral CD 8 ( + ) T epitopes into MHC class 1 presenting cells and induction of protective antiviral cytotoxic T cell ( CTL ) responses . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
The role of CD 40 ligand ( CD40L ) in CD 8 T cell activation was assessed by tracking antigen specific T cells in vivo using both adoptive transfer of T cell receptor transgenic T cells and major histocompatibility complex ( MHC ) class 1 tetramers . ^^^ The mucosal CTL response of transferred CD 8 T cells was MHC class 2 and interleukin 12 independent . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
These results prove that MHC reactive functions protect CMVs against attack by CD 8 ( + ) T lymphocytes in vivo . . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Here , we show by simultaneous staining with two tetrameric major histocompatibility complex ( MHC ) peptide molecules , that the polyclonal CD 8 ( + ) T cell response against a series of natural variants of the influenza A nucleoprotein epitope is completely dominated by infrequent cross reactive T cells that expand from an original memory population . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
The MHC class 2 ligand lymphocyte activation gene 3 is co distributed with CD 8 and CD 3 TCR molecules after their engagement by mAb or peptide MHC class 1 complexes . ^^^ Here , lateral distribution of the LAG 3 molecules and its topological relationship ( mutual proximity ) to the TCR , CD 8 , CD 4 , and MHC class 1 and 2 molecules were studied in the plasma membrane of activated human T cells in co capping experiments and conventional fluorescence microscopy . ^^^ The supramolecular assemblies described here between LAG 3 , CD 3 , CD 8 and MHC class 2 molecules may result from an organization in raft microdomains , a phenomenon known to regulate early events of T cell activation . . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
In HD mice , which lack mature CD4+ T cells , major histocompatibility complex ( MHC ) class 2 restricted T cells are redirected to the CD 8 lineage independent of MHC class 1 expression . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
OLs , at least in vitro , express MHC class 1 molecules and are susceptible to CD 8 ( + ) T cell mediated cytotoxicity . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
During thymic development the recognition of MHC proteins by developing thymocytes influences their lineage commitment , such that recognition of class 1 MHC leads to CD 8 T cell development , whereas recognition of class 2 MHC leads to CD 4 T cell development . ^^^ The coreceptors CD 8 and CD 4 may contribute to these different outcomes through interactions with class 1 and class 2 MHC , respectively , and through interactions with the tyrosine kinase p56lck ( Lck ) via their cytoplasmic domains . ^^^ These results indicate that CD 8 alpha ' favors the development of CD 8 lineage T cells , perhaps by reducing Lck activity upon class 1 MHC recognition in the thymus . . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Although Ag recognition by T cells was inhibited by mAb against CD 8 and the TCR complex ( anti TCR alphabeta , CD 3 , Vbeta 12 ) , it was not inhibited by mAb directed against MHC class Ia or MHC class 2 molecules . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
The development of T cells results in a concordance between the specificity of the TCR for MHC class 1 and class 2 molecules and the expression of CD 8 and CD 4 coreceptors . ^^^ Here , we show that , by altering the intracellular signaling involving the Erk / MAP kinase pathway , T cells with specificity for MHC class 1 can be diverted to express CD 4 , and , conversely , T cells with specificity for MHC class 2 can be diverted to express CD 8 . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Mice infected with Listeria monocytogenes ( LM ) generate CD 8 effectors specific for f MIGWII , the amino terminus of the bacterial product lemA presented by the class Ib MHC molecule H 2 M3wt . lemA has several distinctive properties : 1 ) it is readily presented as an exogenous Ag in the absence of bacterial infection ; 2 ) it is processed by a TAP independent pathway , which is sensitive to chloroquine , pepstatin , and brefeldin ; and 3 ) the immunogenic portion of the molecule is extremely resistant to proteolytic degradation even by proteinase K . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Single and dual immunohistochemical staining in various combinations with monoclonal antibodies against CD 45 , CD 4 , CD 8 , major histocompatibility complex ( MHC ) class 2 , CD11c , NLDC 145 , and a variety of macrophage markers was performed . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Additionally , development of CD 8 lineage T cells in an MHC class 1 restricted TCR transgenic background is sensitive to the dosage of E 47 . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Class 1 MHC restricted CTL in wild type mice were not dependent on CD 4 ( + ) cells , suggesting that the failure of CTL in the absence of B7s is due to a lack of B 7 costimulation directly to the CD 8 ( + ) CTL . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
The MHC class 2 binding competent hCD 4 ( R240AhCD4 ) rescues the number and helper activity of hCD 4 ( + ) CD 8 ( ) single positive ( SP ) mature T cells in mCD 4 ( / ) mice . ^^^ In contrast , the MHC class 2 binding deficient F43I hCD 4 mutant can not facilitate normal differentiation of double positive thymocytes to CD 4 ( + ) CD 8 ( ) SP thymocytes . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
A model CD 8 ( + ) T cell epitope of ovalbumin was incorporated at 10 of these permissive sites along the toxin molecule , and the capacity of ACT constructs to penetrate into cell cytosol and deliver the epitope into the major histocompatibility complex ( MHC ) class 1 antigen processing and presentation pathway was examined . ^^^ While all six constructs bearing the epitope within the Hly portion of ACT failed to deliver the epitope to the MHC class 1 molecules , all four toxoids with inserts within different permissive sites in the AC domain efficiently delivered the epitope into this cytosolic pathway , giving rise to stimulation of a specific CD 8 ( + ) T cell hybridoma . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Persistence of memory CD 8 T cells in MHC class 1 deficient mice . ^^^ In addition , memory CD 8 T cells , unlike nave cells , divided without MHC T cell receptor interactions . ^^^ Thus , after nave CD 8 T cells differentiate into memory cells , they evolve an MHC class 1 independent `` life style ' ' and do not require further stimulation with specific or cross reactive antigen for their maintenance . . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
The available evidence favors the notion that such DP T cells in swine are comprised predominantly of MHC class 2 restricted memory CD4+ helper T cells that after activation have acquired the ability to express the CD8alpha chain and then to maintain this DP phenotype . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
OBJECTIVE : To evaluate effect of age and parity on distribution and number of cells expressing major histocompatibility complex ( MHC ) class 2 , CD 4 , or CD 8 molecules in the endometrium of mares during estrus . ^^^ PROCEDURES : Endometrial specimens collected from the uterine body and horns during estrus were stained by use of the avidin biotin peroxidase method , using monoclonal antibodies against equine MHC class 2 , CD 4 , and CD 8 molecules . ^^^ CONCLUSIONS AND CLINICAL RELEVANCE : The increased likelihood for endometritis to develop in mares as they age can not be explained by a decrease in number of cells expressing MHC class 2 , CD 4 , or CD 8 molecules within the endometrium . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
CD 40 signaling along with soluble Ag immunization also induced expansion of secondary lymphoid and intestinal mucosal endogenous OVA specific CD 8 T cells as detected by MHC tetramer reactivity . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
The function of the CD 8 alpha chain for binding to MHC class 1 and associating with the tyrosine kinase p56lck was demonstrated with CD 8 alpha alpha homodimers . ^^^ In contrast to CD 8 alpha alpha , CD 8 beta beta is unable to bind MHC class 1 in a cell cell adhesion assay . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
The gross morphological damage score was evaluated and the expression of CD 4 , CD 8 , CD11a , CD11b , CD 25 , CD 54 and class 2 major histocompatibility complex ( MHC ) antigen was investigated by immunohistochemical methods . 3 . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Antibody blocking assays confirmed that TIL 1383 1 was MHC class 1 restricted , and the CD 4 and CD 8 coreceptors did not contribute significantly to antigen recognition . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Tissue activation of liver and kidney was assessed by staining of immediate early gene products ( IEG : FOS , JUN ) , and inflammatory markers ; cell adhesion molecules ( Intercellular adhesion molecule 1 , vascular cell adhesion molecule 1 ) , leukocyte infiltrates ( CD 45 , T cell receptor , CD 8 , CD 4 ) , and MHC class 2 . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
MT also reduces the proliferative response of CTLL 2 cells to cytokines , and decreases the level of major histocompatibility complex ( MHC ) Class 1 and CD 8 molecules detectable on the surface of lymphocytes , while having no significant effect on the level of CD 4 . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
A number of general principles can be derived for the structure of the alpha beta TCR and its interaction with peptide major histocompatibility complex ( pMHC ) in class 1 systems , as well as interaction of the CD 8 co receptor with MHC . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Soluble human MHC class 1 molecules induce soluble Fas ligand secretion and trigger apoptosis in activated CD 8 ( + ) Fas ( CD 95 ) ( + ) T lymphocytes . ^^^ In the present study , we have evaluated the apoptotic effect of soluble human MHC class 1 ( sHLA 1 ) antigens on CD 8 ( + ) T lymphocytes . sHLA 1 antigens and beta ( 2 ) microglobulin free HLA class 1 heavy chains , isolated from serum , induced apoptosis on phytohemagglutinin activated CD 8 ( + ) T lymphocytes in autologous and allogeneic combinations . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Immunohistochemistry showed enhanced staining of hepatic major histocompatibility complex ( MHC ) 2 , CD 4 , and CD 8 in portal areas of ANIT treated animals . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
CTL stimulated with idiotype loaded DC showed strong specific , CD 8 mediated , and major histocompatibility complex ( MHC ) class 1 restricted cytotoxicity against autologous heavy and light chain idiotype . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Recognition of these peptide MHC complexes stimulates TNF production by antigen specific CD 8 ( + ) T cell lines . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
MHC molecules are normally required for the development of thymocytes from the CD 4 ( + ) CD 8 ( + ) double positive to the CD 4 or CD 8 single positive stage . ^^^ Here we show that mitogenic plant lectins can substitute for MHC molecules in driving the differentiation of phenotypically and functionally mature CD 4 as well as CD 8 T cells . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
TCR internalization induced by peptide / MHC ligands requires the transmembrane domains of alphabeta chains of TCR , but not the expression of CD 8 and Thy 1 molecules . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Gag specific cytotoxic T lymphocyte ( CTL ) responses were detected in all MVA gag pol immunized macaques by both functional assays and flow cytometric analyses of CD 8 ( + ) T cells that bound a specific MHC complex class 1 peptide tetramer , with levels peaking after the second immunization . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Proliferative responses to O . ostertagi L 3 antigen extract were restricted to a CD2+ CD 4 CD 8 cell population and MHC II+ cells different from B cells were of major importance . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
This paper presents a standardized gating protocol , and average values and ranges observed for normal canine and feline blood lymphocytes using commercially available antibodies to cell surface markers for CD 5 , CD 3 , CD 4 , CD 8 , MHC 2 , and B lymphocytes . ^^^ The averages for these markers on gated lymphocytes were as follows : Canine CD 5 83 . 3 % , Canine CD 4 45 . 0 % , Canine CD 8 28 . 8 % , Canine MHC 2 98 . 0 % , Canine B Cell 12 . 9 % , Canine CD4 / CD8 ratio 1 . 87 , Feline T lymphocytes 77 . 3 % , Feline CD 4 44 . 5 % , Feline CD 8 25 . 7 % , Feline B Cell 24 . 1 % , Feline CD4 / CD8 Ratio 1 . 75 . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Postexposure challenge with recombinant vaccinia viruses expressing gammaHV 68 lytic cycle epitopes massively increased the magnitude of the gammaHV 68 specific CD 8 ( + ) population detectable by staining with tetrameric complexes of MHC class 1 glycoprotein + peptide , or by interferon gamma production subsequent to in vitro restimulation with peptide . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
To evaluate its immunological effect on tumor sites , an immunohistochemical study was performed using monoclonal antibodies against MHC class 1 , CD 8 and ED 2 antigens after rrIFN gamma treatment . ^^^ Although the immunohistochemical study showed no clear increase in the staining of MHC class 1 or CD 8 antigens on tumors following rrIFN gamma treatment , immunostaining for ED 2 antigen , which is known to be expressed on perivascular cells with MHC class 2 antigen , revealed a clear increase in the number of infiltrating positive cells within the tumors . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
In wild type mice , both CD 4 and CD 8 cell numbers increased in the gut following Salmonella challenge , together with increased expression of major histocompatibility complex ( MHC ) 2 and vascular cell adhesion molecule 1 ( VCAM 1 ) . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Dietary lutein also increased the percentages of CD4+ and CD21+ lymphocytes on Week 12 but had no significant effect on pan T , CD 8 and MHC class 2 markers . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
However , during secondary challenge with a heterologous influenza virus , the local infiltration with virus specific , MHC class 1 restricted CD 8 ( + ) T cells was significantly decreased compared to that of nontransgenic littermates . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Previous work has indicated that an important component for the initiation of autoimmune insulin dependent diabetes mellitus ( IDDM ) in the NOD mouse model entails MHC class 1 restricted CD 8 T cell responses against pancreatic beta cell Ags . ^^^ However , unless previously activated in vitro , such CD 8 T cells have previously been thought to require helper functions provided by MHC class 2 restricted CD 4 T cells to exert their full diabetogenic effects . ^^^ In this study , we show that IDDM development is greatly accelerated in a stock of NOD mice expressing TCR transgenes derived from a MHC class 1 restricted CD 8 T cell clone ( designated AI 4 ) previously found to contribute to the earliest preclinical stages of pancreatic beta cell destruction . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
These changes were shown to occur in LCMV TCR transgenic cells as well as virus specific CD 8 T cells in C57BL / 6 and BALB / c mice identified by MHC class 1 tetramers . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Thymocytes carrying MHC class 1 restricted TCRs differentiate into CD 8 T cells , while those recognizing MHC class 2 become CD 4 T cells . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Activated CD 8 ( + ) T cells are able to induce cytolysis of infected cells ( perforin and CD 95 CD95L mediated pathways ) and also elaborate cytokines , including IFN gamma and TNF after appropriate MHC class 1 peptide recognition . ^^^ New technologies for the detection of antigen specific CD 8 ( + ) T cells , including tetrameric MHC class 1 peptide complexes , intracellular IFN gamma staining and IFN gamma ELISPOT analysis have revised our understanding of the magnitude of the CD 8 ( + ) T cell response to infection . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Role of MHC class 1 expression and CD 8 ( + ) T cells in the evolution of iodine induced thyroiditis in NOD H 2 ( h 4 ) and NOD mice . ^^^ Histological analyses of the thyroids show that following 1 week of iodine administration MHC class 1 expression is elevated on thyroid follicular cells and CD 4 ( + ) and CD 8 ( + ) T cells have begun to infiltrate the gland . ^^^ Depletion of CD 8 ( + ) T cells at early stages of disease induction inhibited not only thyroid infiltration and autoantibody production but also reduced the levels of MHC expression in the thyroid , suggesting that cytokine production by infiltrating lymphocytes was responsible for the increased MHC expression . . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
However , the mature T cells that emerge express only the CD 4 ( MHC class 2 binding ) or CD 8 ( MHC class 1 binding ) co receptor that complements the MHC class specificity of the T cell receptor . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
In addition , in vitro and in vivo murine xenogeneic immune responses to the human testicular xenografts were characterized using MHC class 1 , MHC class 2 , CD 4 , CD 8 , CD4 / 8 knockout mice . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Neuropathology is instead colocalized with microglial proliferation and expression of MHC class 1 and class 2 , ICAM , CD 4 and CD 8 molecules . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
In contrast , sCR 1 treatment did not affect the number of graft infiltrating CD 4 ( + ) and CD 8 ( + ) T cells , CD 45 ( + ) B cells , ED 1 ( + ) and ED 3 ( + ) macrophages , or immune activation with expression of IL 2Ralpha or MHC class 2 . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
The popliteal lymph node response to streptozotocin is under type 1 , MHC class 1 restricted , CD 8 ( + ) T cell control . ^^^ In order to determine whether PLN responses involve CD 4 ( + ) or CD 8 ( + ) T cells , the effects of streptozotocin ( STZ ) , a prototypic immunotoxic compound , were analyzed after injection into the hind footpad of C 57 BL / 6 mice and major histocompatibility complex ( MHC ) class 1 or 2 deficient mice . ^^^ These results suggest that PLN responses to STZ are under the control of type 1 , MHC class 1 restricted , CD 8 ( + ) T cells . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Tumor protective immunity was mediated by MHC class 1 antigen restricted CD 8 ( + ) T cells that secreted T ( H ) 1 cytokine IFN gamma and induced tumor rejection and growth suppression after a lethal challenge with B16G3 . 26 murine melanoma cells . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Role of CD8beta domains in CD 8 coreceptor function : importance for MHC 1 binding , signaling , and positive selection of CD8+ T cells in the thymus . ^^^ We now demonstrate that the CD8beta extracellular domain increases the avidity of CD 8 binding to MHC 1 , and that the intracellular domain of CD8beta enhances association with two intracellular molecules required for TCR signal transduction , Lck and LAT . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Eighty percent of donor chimeric cells were B lymphocytes ( MHC class 1 and 2 positive , Fc receptor positive , CD45 / B220 positive but negative for CD 4 , CD 8 and Thy 1 . 2 ) and 20 % were sorted in the macrophage monocyte population . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Furthermore , the influence of MHC class 1 genotype on the generation of both IFN gamma producing CD 4 ( + ) and CD 8 ( + ) T cells helps explain the major impact of the H 2D gene on recovery from FV disease . . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
We have shown previously that IFN gamma pretreatment of human epidermal cells ( ECs ) cultured in vitro partially reverses down regulation of surface MHC class 1 by HSV infection , allowing recognition by CD 8 CTLs , and that HSV immediate early ( IE ) / early ( E ) proteins are the predominant targets for CD 8 CTLs . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Dietary lutein increased ( P < 0 . 05 ) lymphocyte proliferative response to all three mitogens and increased the percentages of cells expressing CD 5 , CD 4 , CD 8 and major histocompatibility complex class 2 ( MHC 2 ) molecules . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
The cell surface molecules CD 4 and CD 8 greatly enhance the sensitivity of T cell antigen recognition , acting as `` co receptors ' ' by binding to the same major histocompatibility complex ( MHC ) molecules as the T cell receptor ( TCR ) . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
However , EBNA 1 is invisible to CD 8 ( + ) cytotoxic T lymphocytes because its Gly / Ala repeat domain prevents proteasome dependent processing for presentation on major histocompatibility complex ( MHC ) class 1 . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
CD 8 ( + ) cytotoxic T lymphocytes ( CTLs ) recognize antigen in the context of major histocompatibility complex ( MHC ) class 1 molecules . ^^^ In this report , we have examined the in vitro memory CTL response of H 2 ( d ) haplotype murine CD 8 ( + ) T lymphocytes specific for a dominant and subdominant epitope of influenza hemagglutinin using activation marker expression and staining with soluble tetrameric MHC peptide complexes . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
The number of macrophages and MHC class 2 positive cells were counted 3 days , 7 days , 2 weeks , 4 weeks , and 8 weeks posttransection ; also CD 4 , CD 8 , IL 2 receptor positive cells , B cells , and the axonal sprouting were studied . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Efficient detection and immunomagnetic sorting of specific T cells using multimers of MHC class 1 and peptide with reduced CD 8 binding . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Access to this problematic area has been greatly enhanced by the recent development of tetrameric complexes of MHC class 1 glycoprotein + peptide ( tetramers ) for the direct staining of freshly isolated , antigen specific CD 8 ( + ) T cells . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Intradermal inoculation of plasmids expressing antigens that contain MHC class 1 restricted epitopes leads to the induction of specific CD 8 ( + ) cytotoxic T lymphocytes ( CTL ) . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
We evaluated the possible roles and interactions of CD 8 ( + ) T cells during NSV encephalitis and demonstrated that MHC class 1 antigen ( H2K / D ) was expressed on endothelial cells , inflammatory cells , and ependymal cells after intracerebral inoculation of NSV . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Ten of these TCL were CD 8 ( + ) and lysed the inoculates in a MHC Class 1 restricted manner . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
We have generated an isolated alpha 3 domain of a murine MHC class 1 molecule and evaluated the contribution of this domain to binding with the MHC class 1 light chain , beta2m , and CD 8 . ^^^ Our results are relevant for models of MHC folding and CD 8 MHC function . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Mice immunised with human epithelial mucin MUC 1 coupled to oxidised mannan produce MUC 1 specific MHC Class 1 restricted CD 8 ( + ) cytotoxic T cells and are completely protected from the development of MUC 1 ( + ) tumours ; such therapy may be applicable to humans . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
During a 5 day culture in XLCM in the absence of thymic stromal cells , DP thymocytes from normal mice and MHC double knockout mice ( lack SP thymocytes ) proliferate , expand , and differentiate into several ( alphabetaTCR ( + ) , NK1 . 1 ( + ) alphabetaTCR ( + ) , and gammadeltaTCR ( + ) ) subsets of CD 4 ( + ) and predominantly CD 8 ( + ) SP thymocytes . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
We previously demonstrated that the MAIDS resistant H 2 ( d ) strains BALB / cByJ and C57BL / KsJ generate MHC class 1 ( K ( d ) ) restricted virus specific CD 8 ( + ) cytolytic T lymphocytes ( CTLs ) that lyse cells expressing either defective or ecotropic gag proteins . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Furthermore , we provide evidence for a lung cancer associated , MHC class 1 bound peptide antigen ( s ) that reconstitutes the epitope recognized by a lung cancer specific CD 8 ( + ) T cell line derived from a patient with lung cancer . . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Tissue resident CD 8 alpha+ DC , freshly isolated from Flt3L treated mice , existed primarily as immature cells ( CD11c+ , CD11blow , CD 40 / low , CD80low , CD86low , MHC class IIlow ) , consistent with previous observations regarding bulk DC freshly isolated from nonlymphoid tissues . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Both intrahepatically and intramuscularly DNA inoculated mice developed CD 8 ( + ) , MHC class 1 restricted CTL responses that recognized the peptide pulsed as well as HCV proteins expressing target cells . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Additionally , the expression of receptors for CD 14 and MHC class 2 on leucocytes , and the expression of receptors for CD 4 , CD 8 , WC 1 , IL2R and B cells on lymphocytes was measured in mammary secretions by flow cytometric analyses . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Amino acid residues of the alpha 2 and alpha 3 domains that are supposed to be involved in the binding of MHC class 1 to CD 8 molecule , were largely conserved in Mamo 1 genes . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Such determinant hierarchy has been well characterized for MHC class 2 restricted CD 4 cell immunity , but is less well understood for class 1 restricted CD 8 cell responses . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
After o / n incubation in GM CSF , both CD8alpha and CD8alpha+ FL liver DC exhibited marked up regulation of surface MHC class 2 and costimulatory molecules , and acquired potent stimulatory activity for Th 1 ( mainly ) and Th 2 cells . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Purified donor CD 4 ( + ) cells were also able to induce these rapid responses , indicating that activated donor CD 4 ( + ) cells expressing allogeneic MHC molecules were able to effectively stimulate responses by both recipient CD 8 ( + ) and B cells . . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
The specificity and sensitivity of this assay , staining of antigen activated lymphocytes ( SAAL ) , was comparable to that of surface staining with major histocompatibily class ( MHC ) 1 peptide tetramers or of staining of peptide re stimulated CD 8 ( + ) T cells for intracellular IFN gamma . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
However , a unique feature of MHV 68 induced IM is a prominent MHC haplotype independent expansion of CD 8 ( + ) T cells , the majority of which utilize 5 ( beta ) 4 chains in their alphabetaTCR . ^^^ Taken together with previous studies showing an apparent MHC independence , the data suggest that a novel ligand , distinct from conventional sAg and peptide MHC , drives proliferation of 5 ( beta ) 4 ( + ) CD 8 ( + ) T cells . . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Selective MHC class 1 expression by bone marrow derived cells does not lead to an accumulation of CD 8 beta+ cells in beta 2m / mice . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Rejected specimens revealed T lymphocyte infiltration ( CD 4 : CD 8 , 2 : 1 ) with strong HLA DR ( MHC class 2 ) expression . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Mice immunised with oxidised mannan MUC 1 fusion protein ( M FP ) develop MHC restricted CD 8 ( + ) cytotoxic T cells . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
In vivo studies using mice with targeted mutations in CD 8 or MHC class 2 or depleted of CD 8 or CD 4 lymphocyte subsets demonstrate that tumour regression following therapeutic hspE 7 immunization is CD 8 dependent and CD 4 independent . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
The finding that macrophages resist the viral control of MHC 1 dependent antigen presentation reconciles the paradox of efficient induction of CMV specific CD 8 ( + ) CTL in vivo despite extensive potential of CMVs to subvert MHC class I . . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
The development of the majority of CD 8 ( + ) NKT cells expressing TCRalpha / beta , but not TCRgamma / delta , depended on classical MHC class 1 . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
A series of bulk antigenic formats ( freeze thaw lysate , trifluoroacetic acid lysate , extracted membranes , affinity purified MHC class 1 and class 2 presented peptides , acid eluted peptides ) prepared from EBV B LCLs were tested for their ability to stimulate EBV B LCL reactive CD 4 ( + ) and CD 8 ( + ) T lymphocytes in vitro when pulsed onto autologous dendritic cells ( DCs ) . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
We conclude that the relative affinity of TCR for thymic self peptide / class 1 MHC is a critical parameter in determining fate of CD 8 ( + ) cells during thymic selection . . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
The analogs were designed from a local surface region on the CD 8 alpha chain directly adjacent to the bound MHC class 1 , to block the protein associations in the T cell activation cluster that occur upon stimulation of the cytotoxic T lymphocytes ( CTLs ) . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
We also demonstrated that in vitro the B subunit mediates the delivery of various exogenous CD 8 T cell epitopes into the conventional MHC class 1 restricted pathway , as this process is inhibited by brefeldin A and lactacystin and requires a functional TAP system . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
These results suggest that HLA G exerts a direct suppressive effect on CD 4 ( + ) T lymphocytes , even in the absence of the CD 8 ( + ) cells with which other human MHC class 1 molecules are thought to interact . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Cytotoxicity against EL 4 lymphoma was not MHC class 1 restricted , and was not decreased after depletion of CD 8 ( + ) cells from the effector cell population . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
This assay detects primarily CD 8 T cells and shows good correlation with MHC class 1 tetramer staining of cytotoxic T lymphocytes . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Transgenic mice expressing the human class 1 MHC antigen HLA B 27 in conjunction with human CD 8 molecules produced vigorous HLA restricted CTL responses to MV antigens , paralleling those in MV infection of humans . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Immunization with autologous gp 96 elicited MHC 1 restricted , tumor specific CD 8 ( + ) T lymphocytes in 6 / 12 patients immunized . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
The protection by CTB MBP was associated with a dramatic reduction in the number of leukocytes staining for CD 4 , CD 8 , IL 2R or MHC class 2 in the spinal cord as examined by immunohistochemistry . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
High frequencies of circulating IFN gamma secreting CD 8 cytotoxic T cells specific for a novel MHC class 1 restricted Mycobacterium tuberculosis epitope in M . tuberculosis infected subjects without disease . ^^^ MHC class 1 restricted CD 8 cytotoxic T lymphocytes ( CTL ) are essential for protective immunity to Mycobacterium tuberculosis in animal models but their role in humans remains unclear . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Investigations on viral protein expression by studying mRNA and protein distribution together with the characterization of CD 4 and CD 8 inflammatory cells and MHC class 2 up regulation revealed a biphasic disease process . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Regression of MHC class 2 negative MCA 205 fibrosarcomas occurs optimally following adoptive transfer of both CD 4 and CD 8 tumor sensitized T cells , indicating an important function for tumor infiltrating APC . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Experiments using mice in which CD 4 ( + ) T cells were eliminated , either by Ab depletion or by gene knockout of the MHC class 2 beta chain ( MHC 2 KO ) , revealed that priming of therapeutic CD 8 ( + ) effector T cells following vaccination with a GM CSF transduced B16BL6 D 5 tumor cell line occurred independently of CD 4 ( + ) T cell help . ^^^ In contrast , adoptive transfer of CD 8 ( + ) T cells to tumor bearing MHC 2 KO mice improved survival , but eventually all MHC 2 KO mice succumbed to metastatic disease . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
A substantial proportion of CD 8 ( + ) T cells activated during L . monocytogenes infection of C57BL / 6 mice are restricted by the MHC class Ib molecule H 2 M3 . ^^^ This is the first example of CD 8 ( + ) T cell mediated , TAP independent protection against a pathogen in vivo , confirming the importance of alternative MHC class 1 processing pathways in the antibacterial immunity . . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Thus , susceptibility and resistance depend upon the functional composition of CD 4 ( + ) and CD 8 ( + ) T cells governed by the MHC haplotype . . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Thus , hyporesponsiveness can be induced in a subset of CD 8 T cells by recognition of peptides derived from K ( b ) through both the direct and indirect pathways of Ag recognition , while CD 4 T cell hyporesponsiveness to MHC class 1 disparate grafts occurs only through the indirect pathway of Ag recognition . . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
For any of these activating stimuli , CD8alpha ( + ) DC consistently display the highest proportion of HEL loaded MHC class 2 molecules . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Macrophages incubated in vitro with OVA archaeosomes strongly stimulated cytokine production by OVA specific CD 8 ( + ) T cells , indicating that archaeosomes efficiently delivered entrapped protein for MHC class 1 presentation . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Infection of B 6 mice with the intracellular pathogen Listeria monocytogenes ( LM ) results in the activation of CD 8 ( + ) T cells that respond to Ag presented by both MHC class Ia and class Ib molecules . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
The FL in vitro derived DCs , when treated with interferon alpha or lipopolysaccharide during the final 24 hours of culture , expressed an activated phenotype that included up regulation of MHC class 2 , CD1d , CD8alpha , CD 80 , CD 86 , and CD 40 . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
To study the impact of an MHC class 2 negative environment on T cell immune reconstitution , we have analyzed the phenotypical and functional characteristics of FACS sorted cultured CD 4 ( + ) and CD 8 ( + ) T cells in two Bare Lymphocyte Syndrome ( BLS ) patients before and after allo BMT . ^^^ In addition , reduced MHC class 2 expression was observed in CD 8 ( + ) T cells of both recipients early after allo BMT , irrespective of the T cell chimerism pattern . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
A significant decrease in proliferation of TNFR 1 deficient CD8+ responder cells , but not of TNFR 2 deficient CD 8 responder T cells was observed after stimulation with MHC class 1 disparate bm 1 stimulator cells . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
ICP 47 has been shown to inhibit the transporter associated with antigen presentation , thus blocking major histocompatibility antigen 1 ( MHC class 1 ) mediated antigen presentation to CD 8 ( + ) T cells . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
METHODS : A four color flow cytometric assay that simultaneously measures tetramer , CD 3 , CD 8 , and CD 14 was used to investigate the sensitivity and specificity of MHC class 1 tetramer staining . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Whereas CD 8 ( + ) T cells in peripheral lymphoid organs use CD8alpha / beta and are selected on MHC class Ia molecules , a majority of iIELs use CD8alpha / alpha . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Many viral infections induce a broad repertoire of CD 8 ( + ) T cell responses that initiate recognition and elimination of infected cells by interaction of TCRs with viral peptides presented on infected cells by MHC class 1 proteins . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
In this work , we have studied the role of the MHC class Ib molecules in the selection and maintenance of CD 8 ( + ) T splenocytes . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Although several nonprotein ligands have been identified for CD 1 restricted CD 8 ( + ) CTLs , epitopes for classical MHC class 1 restricted CD 8 ( + ) T cells , which most likely represent a majority among CD 8 ( + ) T cells , have remained ill defined . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
To separate the contributions of CD 4 ( + ) and CD 8 ( + ) direct pathways , we transplanted tracheas from knockout mice lacking major histocompatibility complex ( MHC ) class 1 or 2 antigens . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
According to current consensus , CD 8 ( + ) T cell responses are focused upon short peptide sequences ( 8 11 amino acids ) presented by MHC class 1 molecules . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
CD 8 ( + ) T lymphocytes , which are major immune effectors , require primary stimulation by dendritic cells ( DC ) presenting MHC class 1 molecule bound epitopes . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Cytotoxic activity of the CTL clone against the HER 2 expressing cell line bearing HLA A 2402 was blocked by antibodies against CD 3 , CD 8 , HLA A 24 or MHC class 1 , and was also inhibited by the addition of excess HER2p63 pulsed C1R bearing HLA A 2402 . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Splenocytes were analyzed for the following cell surface markers : CD 3 , TCR alpha / beta , CD 4 , CD 8 and major histocompatibility complex ( MHC ) Class 2 . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
The cytotoxic activity against healthy cardiomyocytes was myocyte specific , induced by CD 8 lymphocytes and major histocompatibility complex ( MHC ) restricted . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Molecular interactions of coreceptor CD 8 and MHC class 1 : the molecular basis for functional coordination with the T cell receptor . ^^^ In recent years , substantial progress has been made towards understanding the molecular basis for CD 8 binding to class 1 MHC and the coreceptor ' s role in cytotoxic T cell activation . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Functional characterization of CD 8 ( + ) antigen specific cytotoxic T lymphocytes after enrichment based on cytokine secretion : comparison with the MHC tetramer technology . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Multiple model systems have demonstrated that negatively selected thymocytes can be deleted during the immature CD 4 ( + ) CD 8 ( + ) CD 3 ( low ) stage after high affinity interaction of T cell receptors ( TCRs ) with antigen : major histocompatibility complex ( MHC ) complexes . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Bone marrow derived antigen presenting cells ( APCs ) take up cell associated antigens and present them in the context of major histocompatibility complex ( MHC ) class 1 molecules to CD 8 ( + ) T cells in a process referred to as cross priming . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
The molecular events triggered by MHC recognition and how they lead to the emergence of mature CD 4 and CD 8 lineage thymocytes are not yet understood . ^^^ To address these questions , we have examined what signals are necessary to drive the development of CD 8 lineage thymocytes in TCRalpha ( ) mice in which TCR / MHC engagement can not occur . ^^^ These data indicate that survival and lineage commitment represent distinct , parallel pathways that occur as a consequence of MHC recognition , both of which are necessary for the development of mature CD 8 lineage T cells . . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
These results demonstrate a previously unrecognized , MHC class 1 restricted , CD 8 ( + ) CTL response to a major secreted Ag of mycobacteria and supports the use of Ag85A as a candidate vaccine against tuberculosis . . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
The CD 8 glycoprotein serves as a coreceptor for T cell receptor ( TCR ) mediated recognition of peptides associated to MHC class 1 . ^^^ In CD8alpha deficiency , MHC class 1 restricted cytotoxic T lymphocytes ( CTL ) are absent and lineage commitment to CD 8 cells is impaired . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
We have reported previously that the cytolytic activity of murine CD 8 ( + ) cytotoxic T lymphocytes ( CTL ) specific for HIV 1 gp 160 envelope glycoprotein was markedly inhibited by brief exposure to the free minimal antigenic peptide ( 1 10 : 10mer peptide from gp 160 ) by direct binding to class 1 MHC molecules of specific CTL in the absence of antigen presenting cells ( APC ) . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
TCRAV segments contribute significantly to MHC restriction as illustrated by their general preference for either the CD 4 or CD 8 T cell subset and additional , MHC allele specific overselection during T cell differentiation . ^^^ The 10 fold over representation of the TCRAV8S2 ( VA8S2 ) segment in CD 8 over CD 4 T cells by the RT 1 ( f ) haplotype of LEW . 1F rats provides the most striking example of MHC allele specific overselection of a VA segment reported so far . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
To test this concept in a relevant disease model we sought to identify multiple simian immunodeficiency virus ( SIV ) derived CD 8 ( + ) epitopes bound by a single nonhuman primate major histocompatibility complex ( MHC ) class 1 molecule . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Class 1 MHC tetramers have proven to be invaluable tools for following and deciphering the CD 8 ( + ) T cell response , but the development of similar reagents for detection of CD 4 ( + ) T cells based on class 2 MHC proteins has been more difficult . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Using recombinant MHC class 1 tetramers , we isolated Ag specific CD 8 ( + ) T cells from mice infected with lymphocytic choriomeningitis virus at various time points and performed semiquantitative RT PCR . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
These data unambiguously indicate that the functional effect of Ly49A as an MHC class 1 specific NK cell receptor is mediated by binding to a concave region formed by three structural domains of H 2D ( d ) , which partially overlaps the CD 8 binding site . . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
In the present study , we have created transgenic mice where MHC class 1 expression is driven selectively in dendritic cells and provide direct in vivo evidence that dendritic cells are sufficient to cross present exogenous self Ags and induce Ag specific cell division of CD 8 positive T cells . . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
The importance of CD 4 ( + ) T cells in the induction of an optimal antitumor immune response has largely been attributed to their ability to provide costimulatory signals for the priming of MHC class 1 restricted CD 8 ( + ) CTL . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
The CD 8 T cell response to vesicular stomatitis virus infection was characterized in the spleen and intestinal mucosa using MHC tetramers . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
METHODS : To determine whether select T cell populations regulate intrarenal nephritogenic cytokines ( CSF 1 , GM CSF , and TNF alpha ) and renal disease , we compared MRL Fas ( lpr ) mice that are genetically deficient in T cell receptor ( TCR ) alpha beta T cells , CD 4 T cells , and major histocompatibility complex class 1 ( MHC class 1 ) , lacking CD 8 and double negative ( DN ) T cells , with wild type mice . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
In accordance with their lack of MHC restriction , most mouse and human gamma delta T cells express neither the CD 4 nor CD 8 ( alpha beta ) coreceptor . ^^^ In striking contrast , up to 80 % of splenic rat gamma delta T cells express the CD8alpha beta isoform of CD 8 , which for the alpha beta T cell subset serves as a marker for MHC class 1 restricted cells . ^^^ In summary , CD 8 expressed by rat gamma delta T cells is a molecule with the potential to act as a coreceptor , but its expression gives no indication for antigen recognition analogous to that of MHC class 1 restricted alpha beta T cells . . ^^^ Expression of functional CD8alpha Beta heterodimer on rat gamma delta T cells does not correlate with the CDR 3 length of the TCR delta chain predicted for MHC class 1 restricted antigen recognition . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Definition of a novel binding site on CD 8 cells for a conserved region of the MHC class Ib molecule Qa 1 that regulates IFN gamma expression . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
CD 8 ( + ) and CD 8 ( ) T cell lines expressing the same antigen specific receptor [ the 2C T cell receptor ( TCR ) ] were compared for ability to bind soluble peptide MHC and to lyse target cells . ^^^ The 2C TCR on CD 8 ( ) cells bound a syngeneic MHC ( K ( b+ ) ) peptide complex 10 100 times less well than the same TCR on CD 8 ( + ) cells , and the CD 8 ( ) 2C cells lysed target cells presenting this complex very poorly . ^^^ Surprisingly , however , the CD 8 ( ) cells differed little from CD 8 ( + ) cells in ability to bind an allogeneic MHC ( L ( d+ ) ) peptide complex and to lyse target cells presenting this complex . ^^^ The CD 8 ( + ) / CD8 ( ) difference provided an opportunity to estimate how long TCR engagements with peptide MHC have to persist to initiate the cytolytic T cell response . . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Proliferation of CD 8 ( + ) 2C cells was proportional to the abundance of cognate peptide / MHC complexes and was severely inhibited by injection of anti CD 8 antibody . ^^^ The findings suggest that under uniform lymphopenic conditions , the widely different rates of proliferation of T cells expressing various TCR , or the same TCR in the presence or absence of CD 8 , reflect the strength of interaction between TCR and MHC associated with particular self peptides . . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
In vivo studies using mice with targeted mutations in CD 8 or MHC class 2 or depleted of CD 8 or CD 4 lymphocyte subsets demonstrate that tumor regression following therapeutic HspE 7 immunization is CD 8 dependent and CD 4 independent . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Sibling pair analysis ( SIBPAL ) , relative pair analysis ( RELPAL ) and linkage studies using two different models of inheritance suggested linkage for the MHC and two additional chromosomal regions : chromosome 2 ( D2S443 near CD 8 and IGk ; 2p13 2p11 . 1 ) , and chromosome 15 ( CYP 19 estrogen synthase ; 15q15 ) . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
The intestinal mucosal CD 8 T cell response to infection with Listeria monocytogenes was measured using MHC class 1 tetramers and was compared with the response in peripheral blood , secondary lymphoid tissue , and liver . ^^^ Infection of CD 40 or MHC class 2 deficient mice induced poor CD 8 T cell responses in the intestinal mucosa , but only partially reduced responses in the spleen and liver . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Here we describe the development and lack of self tolerance of CD 8 ( + ) T lymphocytes in transgenic mice expressing MHC class 1 molecules in the thymus exclusively on cortical epithelial cells . ^^^ Despite the absence of MHC class 1 expression on professional antigen presenting cells , normal numbers of CD 8 ( + ) cells were observed in the periphery . ^^^ Upon specific activation , transgenic CD 8 ( + ) T cells efficiently lysed syngeneic MHC class 1 ( + ) targets in vitro and in vivo , indicating that thymic cortical epithelium ( in contrast to medullary epithelium and antigen presenting cells of hematopoietic origin ) is incapable of tolerance induction . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Co expression of Qa 1 ( b ) and D ( k ) on target cells significantly inhibited cytotoxicity of D ( k ) specific cytotoxic T lymphocytes generated by mixed lymphocyte reaction , indicating that Qa 1 ( b ) on antigen presenting cells interacts with CD94 / NKG2A on CD 8 T cells and regulates classical MHC class 1 restricted cytotoxic T cells . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
The ability to generate MHC class 1 restricted CD 8 CTL lines specific for a non mutated p 53 determinant from normal , immunocompetent mice , which display antitumor activity both in vitro and in vivo ( by adoptive transfer ) , may have implications for the immunotherapy of certain p 53 expressing malignancies . . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
The goal of this study was to determine whether HER 2 / neu specific CD 8 T cell immunity could be elicited using HER 2 / neu derived MHC class 2 `` helper ' ' peptides , which contain encompassed HLA A 2 binding motifs . ^^^ These results demonstrate that HER 2 / neu MHC class 2 epitopes containing encompassed MHC class 1 epitopes are able to induce long lasting HER 2 specific IFN gamma producing CD 8 T cells . . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
In addition to this disparity , we find that MHC class Ia restricted CD 8 T cells specific for a dominant L . monocytogenes epitope have different TCR 5 beta repertoires in the spleen and intestinal mucosa of individual mice . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
In adult Xenopus , surface expression of CTX marks a population of MHC class 1 ( ) CD 8 ( + ) immature thymocytes that appears to be the equivalent of the mammalian CD4CD8 double positive subset . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
The structural basis of antigen recognition in cellular immunity has been elucidated through the determination of crystal structures of major histocompatibility complex ( MHC ) molecules bound to antigenic peptides , T cell receptors ( TCR ) , CD 8 and CD 4 co receptors and , most recently , TCRs in complex with peptide MHC ( pMHC ) . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Immunohistochemical staining was performed to identify infiltrating cells ( CD 4 , CD 8 , and ED 1 ) and the expression of MHC class 2 and ICAM 1 . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Lymphocyte subpopulations ( CD 4 and CD 8 T cells , macrophages , and B cells ) and expression of major histocompatibility complex 1 ( MHC 1 ) molecules were assessed by flow cytometry . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Role of MHC class 1 and CD 8 ( + ) T cells in the pathogenesis of chronic rejection . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
The production of IFN gamma by MPT 64 ( 190 198 ) specific CD 8 ( + ) T cells was inhibited by antibodies to MHC class 1 , but not to CD1d . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Subsets of mature murine DC isolated directly from the spleen have been shown to differ in their ability to induce proliferative responses in both primary CD 4 ( + ) and primary CD 8 ( + ) T cells ; the myeloid related CD8alpha ( ) DC induce a more intense or prolonged proliferation of naive T cells than do the lymphoid related DC bearing CD8alpha despite similar expression of MHC and co stimulatory molecules . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Here we show that the developing alphabeta thymocyte which does not express CD 8 at the cell surface has a selective advantage in FTOC with sTCR , thus suggesting that participation of CD 8 in self peptide / MHC recognition confers specificity to T cell selection and results in excessive signaling in thymocytes in spite of the presence of sTCR . . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Moreover , vaccination of H 2I Abeta deficient mice ( lacking major histocompatibility class ( MHC ) 2 molecules and thus devoid of mature CD 4 TCR alphabeta cells ) , of TAP 1 deficient as well as of beta2microglobulin deficient mice ( devoid of conventional CD 8 T cells ) with a sublethal dose of L . monocytogenes and subsequent challenge with rSalmonella control or SODs strain revealed contribution of both MHC class 1 and MHC class 2 dependent immune mechanisms to the control of secondary Salmonella infection . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
The growing evidence that exogenous antigens can also be processed and presented by class 1 MHC molecules has suggested that epitope spreading could occur for CD 8 ( + ) cytotoxic T lymphocyte ( CTL ) responses as well . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
For class 2 MHC restricted presentation to CD 4 T cells , the CD 8 ( ) DC subtypes were more efficient , but for class 1 MHC restricted presentation to CD 8 T cells , the CD 8 ( + ) DC subtype was far more effective . ^^^ The CD 8 ( + ) DC are therefore specialized for in vivo cross presentation of exogenous soluble Ags into the class 1 MHC presentation pathway . . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
These findings demonstrate that the self MHC class 1 gene dosage may regulate the extent of CD 8 ( + ) T cell responsiveness to Ag . . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Therefore , we created a novel fusion of VP 22 with a model Ag , human papillomavirus type 16 E 7 , in a DNA vaccine that generated enhanced spreading and MHC class 1 presentation of AG : These properties led to a dramatic increase in the number of E 7 specific CD 8 ( + ) T cell precursors in vaccinated mice ( around 50 fold ) and converted a less effective DNA vaccine into one with significant potency against E 7 expressing tumors . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
In the case of NK1 . 1 TCRint cells , they were selected by polymorphic MHC antigens , but their MHC restriction to CD 4 or CD 8 antigen was incomplete . ^^^ These results suggest that the development of T lineage lymphocytes and MHC restriction to CD 4 and CD 8 might have occurred in parallell as a phylogenic event , and that NK1 . 1 extrathymic T cells ( i . e . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Ts derive from a population of CD 3 ( + ) CD 8 ( + ) CD 28 ( ) T lymphocytes and specifically recognize the MHC class 1 antigens of the APC used for in vitro immunization . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
The present study has assessed the impact of the intracellular domains of CD 4 and CD 8 on positive selection and lineage direction of MHC class 1 restricted thymocytes . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
These effects on MHC class 1 and class 2 expression can be expected to interfere transiently with adaptive immune responses of the host , mediated by CD 4 and CD 8 T cells , ensuring that the virus has sufficient opportunity for transmission to susceptible contracts . . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Mounting evidence suggests that protective immunity to M . tuberculosis relies on both MHC class 2 restricted CD 4 ( + ) T cells and MHC class 1 restricted CD 8 ( + ) T cells . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Intrathymic self peptide major histocompatibility complex class 2 ( MHC ) molecules shape the T cell repertoire through positive and negative selection of immature CD 4 ( + ) CD 8 ( + ) thymocytes . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Major histocompatibility complex ( MHC ) class 1 restricted CD 8 ( + ) T cells play a critical role in the protective immunity against Mycobacterium tuberculosis ( Mtb ) . ^^^ However , only a few Mtb peptides recognized by MHC class Ia restricted CD 8 ( + ) T cells have been identified . ^^^ M 3 is an MHC class Ib molecule that preferentially presents N formylated peptides to CD 8 ( + ) T cells . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Blocking CD 8 binding using alpha 3 domain mutants of MHC class 1 results in much reduced signaling and reduced killing of the targets . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
This provided evidence for the presence of virus specific CD 4 ( + ) and CD 8 ( + ) memory T cells after acute infection and indicated that ex vivo monocytes from PI , immunotolerant calves stimulated both MHC class 1 and MHC class 2 restricted T cell responses to BVDV . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Major histocompatibility complex ( MHC ) class 1 restricted , tumor associated antigen ( TAA ) specific , CD 8 ( + ) cytotoxic T lymphocytes ( CTL ) were identified in 58 / 76 ( 76 . 32 % ) brain tumors , and usually represented 1 10 % of all cells , but in some cases 30 44 % of the cells were CD 8 ( + ) . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Critical role for CD 8 in binding of MHC tetramers to TCR : CD 8 antibodies block specific binding of human tumor specific MHC peptide tetramers to TCR . ^^^ Recent evidence from transgenic mouse models suggests that CD 8 plays a critical role in TCR binding and activation by peptide MHC complex multimers ( tetramers ) . ^^^ Here we show with a human CTL clone specific for a tumor associated MHC peptide complex that the binding of tetramers to the TCR on these cells is completely blocked by anti human CD 8 Abs . ^^^ Moreover , the staining of CTLs with specific MHC peptide tetramers simultaneously with anti CD 8 Abs was completely blocked with three different anti CD 8 Abs . ^^^ MHC peptide tetramers are widely used today in combination with anti CD 8 Abs for the phenotypic analysis of T cell populations and in the study of T cell responses under various pathological conditions such as infectious diseases and cancer . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
This property has been exploited to design recombinant CyaA toxoids capable of delivering major histocompatibility complex ( MHC ) class 1 restricted CD 8 ( + ) T cell epitopes into antigen presenting cells and to induce specific CD 8 ( + ) cytotoxic T lymphocyte ( CTL ) responses in vivo . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
This report reveals a potential therapeutic role for MHC class 1 restricted peptides in treating autoimmune disease and revisits the notion that the CD 4 and CD 8 inducing determinants on some molecules may benefit from a proximal relationship . . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
The expression of CD 4 , CD 8 , MHC class 2 , interleukin 2 receptor , nitric oxide 2 , and NKR P1A ( NK cells ) positivity was significantly decreased in the liver and skin of BMT rats by MC 1288 . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
This subset is absent in mice that lack beta 2 microglobulin , but not in K ( b ) D ( b ) double knockout mice , indicating that these CD 8 T cells are dependent on nonclassical MHC class Ib molecules . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Biochemical changes induced by TNF alpha in the MHC class 1 pathway were translated into increased sensitivity of TNF alpha treated targets to lysis by CD 8 ( + ) cytotoxic T cells , demonstrating improved presentation of at least certain endogenously processed MHC class 1 restricted peptide epitopes . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
We used RT PCR spectratyping to compare the breadth of the T cell receptor usage in the liver in comparison with the peripheral blood , and applied MHC class 1 tetramer technology to investigate the numbers of HCV specific CD 8 ( + ) cells in the two compartments . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Furthermore , the CD 4 ( + ) CD 8 ( dim ) cytokine responses to CMV were shown to be MHC class 2 dependent . ^^^ Thus , CD 4 ( + ) CD 8 ( dim ) T cells appear to be MHC class 2 dependent , are capable of cytolytic effector activity , and are highly enriched within the CD 4 ( + ) cell populations specific for HCMV and HIV 1 . . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
The tumor protective immunity was mediated by MHC class 1 antigen restricted CD 8 ( + ) T cells together with CD 4 ( + ) T cell help , which was required only for tumor cell killing in the effector phase of the immune response . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
MHC class 1 allele dosage alters CD 8 expression by intestinal intraepithelial lymphocytes . ^^^ We examined the influence of MHC 1 allele dosage on the development CD 8 ( + ) T cells in RAG 2 ( / ) mice expressing the H 2D ( b ) restricted transgenic TCR specific for the male , Smcy derived H Y Ag ( H Y TCR ) . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Activation of CD 8 ( + ) cytolytic T lymphocytes ( CTLs ) by antigen is triggered by the interaction of clonotypic alphabeta T cell receptors ( TCRs ) with antigenic peptides bound to MHC class 1 molecules ( pMHC complexes ) . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
The product of the Lymphocyte Activation Gene 3 ( LAG 3 , CD 223 ) is a high affinity MHC class 2 ligand expressed by activated CD 4 ( + ) and CD 8 ( + ) T cells , which can associate with the T cell receptor ( TCR ) and downregulate TCR signalling in vitro . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
The tetrameric MHC peptide complexes specifically bound to the reactive T cell clone and 0 . 79 % of CD 8 ( + ) T cells in peripheral blood from a seropositive donor . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
As loss of cis mechanism 1 activity occurs around the onset of positive selection , it is possible that events associated with TCR / major histocompatibility complex ( MHC ) interactions and selection are involved in initiating these changes in CD8alpha transcription . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Two distinct routes are followed by CD 8 lineage cells interacting with MHC class 1 molecules , via TCR ( hi ) CD 4 ( + ) CD 8 ( + ) or CD 4 ( + ) CD 8 ( int ) intermediates , while CD 4 lineage cells mature exclusively via a CD 4 ( + ) CD 8 ( int ) stage . ^^^ The distribution of sequences also indicates that CD 4 / CD 8 lineage commitment is not strictly correlated with the class of MHC molecule engaged , and that some mechanism prevents mismatched intermediates from achieving full maturity . . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
The CD 8 co receptor is essential for TCR dependent immune recognition and T cell development involving peptides bound to MHC class 1 ( MHCI ) molecules . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NK1 . 1 ( + ) CD 8 ( + ) T cells show a potent NK like cytotoxic activity against multiple tumor targets , and lysis is independent of major histocompatibility complex ( MHC ) class 1 or non classical MHC class 1 molecules ( Qa , TL ) . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
At week 34 , 6 lambs had higher proportions of CD 8 ( + ) , gammadelta ( + ) , MHC class 2 ( + ) , and L selectin ( LS ( + ) ) BAL cells compared with VP lambs . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Epithelial damage and obliteration were graded histologically , and the number of CD 4 , CD 8 , MHC class 2 expressing cells , macrophages , and B lymphocytes were counted ( mean+SEM ) / high power visual field . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Taken together , these observations demonstrate that the effects of SAg on in vivo humoral immune responses are highly CD 4 ( + ) cell dependent , are substantially CD 8 ( + ) cell independent and can be successfully investigated using human MHC class 2 transgenic mice . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
The ability to monitor vaccine elicited CD 8 ( + ) cytotoxic T lymphocyte ( CTL ) responses in simian immunodeficiency virus ( SIV ) and simian human immunodeficiency virus ( SHIV ) infected rhesus monkeys has been limited by our knowledge of viral epitopes predictably presented to those lymphocytes by common rhesus monkey MHC class 1 alleles . ^^^ We now define an SIV and SHIV Nef CTL epitope ( YTSGPGIRY ) that is presented to CD 8 ( + ) T lymphocytes by the common rhesus monkey MHC class 1 molecule Mamu A * 02 . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
In this study , using a panel of MHC class 1 and / or class 2 restricted peptides , we analyzed CD 4 ( + ) and CD 8 ( + ) T cell responses obtained after i . v . injection of freshly purified peptide pulsed DC subsets . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Hapten addition to an MHC class 1 binding peptide causes substantial adjustments of the TCR structure of the responding CD 8 ( + ) T cells . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Depletion of NK cells did not affect LCMV elicited T cell responses in immunocompetent mice ; however , the presence of NK cells did inhibit CD 4 T cell IFN gamma production , BrdU incorporation , and expansion in infected MHC class 1 and CD 8 T cell deficient beta2M / mice . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Immunohistochemical stain was performed on these wholemounts using monoclonal antibodies to transforming growth factor beta 1 ( TGF beta 1 ) , CD 3 , CD 4 , CD 8 , B lymphocytes , macrophages , dendritic cells and major histocompatibility complex ( MHC ) class 2 antigen . ^^^ Presence of TGF beta 1 , CD 3 , CD 4 , CD 8 , MHC class 2 positive cells , macrophages and dendritic cells were noted at the limbus of both SD rats and Wistar rats . ^^^ A variety of cells including TGF beta 1 , CD 3 , CD 4 , CD 8 , MHC class 2 antigen positive cells , macrophages and dendritic cells are involved in corneal allograft rejection . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Although similar frequencies of Gag peptide specific CD 8 ( + ) T cells were found in LTNP and progressors by either intracellular IFN gamma or MHC class 1 tetramer staining , the breadth of these responses was greater in patients with progressive HIV infection compared with the LTNP group . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Corrigendum to `` The sow endometrium at different stages of the oestrous cycle : studies on the distribution of CD 2 , CD 4 , CD 8 and MHC class 2 expressing ' ' cells . [ Anim . ^^^ The aim of this study was to investigate the distribution of CD 2 ( + ) , CD 4 ( + ) , CD 8 ( + ) lymphocyte subpopulations and MHC class 2 expressing cells in the sow endometrium throughout the oestrous cycle . ^^^ In conclusion , the present study showed a variation in the distribution of T lymphocyte subpopulations ( CD 2 ( + ) , CD 4 ( + ) and CD 8 ( + ) ) and MHC class 2 expressing cells in the sow endometrium during different stages of the oestrous cycle . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
This vaccine , delivered by oral gavage with an attenuated strain of Salmonella typhimurium ( SL 7207 ) , and boosted with an antibody IL 2 fusion protein , induced tumor protective immunity mediated by MHC class 1 antigen restricted CD 8 ( + ) T cells , resulting in eradication of subcutaneous tumors in 100 % of mice and prevention of experimental pulmonary metastases in 75 % of experimental animals . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
In these mice the number of CD 8 SP thymocytes was greatly increased , and this continued to be true even when MHC class 1 was absent . ^^^ Furthermore , even thymocytes carrying MHC class 2 restricted TCR differentiated into the CD 8 SP lineage when AML 1 was overexpressed . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Lymphocyte subsets CD 3 , CD 4 , CD 8 , CD 20 and MHC molecule beta 2 microglobulin ( beta 2 MG ) and HLA DR in liver tissues were studied using immunohistochemical staining . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Gene delivery to DCs provides an intracellular source of antigen for efficient and persistent loading to MHC class 1 molecules capable of activating CD 8 ( + ) CTLs , which play a central role in antitumor immunity . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Manipulation of avidity to improve effectiveness of adoptively transferred CD 8 ( + ) T cells for melanoma immunotherapy in human MHC class 1 transgenic mice . ^^^ In this study , we report the generation of HLA A * 0201 restricted CD 8 ( + ) T cell populations that recognize either tyrosinase ( 369 376 ) or gp 100 ( 209 217 ) from tolerant human class 1 MHC transgenic mice by using single amino acid substituted variant peptides . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Tumor rejection has all the characteristics of a CD 8 ( + ) T cell mediated immune response , as the tumor did not express MHC class 2 and only tumor tissue was the subject of destruction . ^^^ However , analysis of the molecular and cellular mechanisms involved in tumor clearance revealed that perforin , TNF alpha , Fas ligand , MHC class 1 , and CD 8 ( + ) T cells did not play a crucial role in tumor eradication . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Pathogenesis of mousepox in H 2 ( d ) mice : evidence for MHC class 1 restricted CD 8 ( + ) and MHC class 2 restricted CD 4 ( + ) CTL antiviral activity in the lymph nodes , spleen and skin , but not in the conjunctivae . ^^^ It is well known from the studies defined on some other viruses that a correlation , functional link and powerful help exist between MHC class 1 restricted CD 8 ( + ) and MHC class 2 restricted CD 4 ( + ) virus specific cytotoxic T lymphocytes ( CTLs ) . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
The resulting TCR CD 8 adducts exhibit high affinity for major histocompatibility complex ( MHC ) peptide . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
In this model , the disease causing effectors are TCR Vbeta8 . 2+ and each of the required CD 4 and CD 8 regulatory T cell populations are specific for different conserved regions of the Vbeta8 . 2 chain , in the appropriate MHC context . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
The effector cells in most experimental tumor systems are CD 8 ( + ) T cells that recognize MHC class 1 binding peptides derived from molecules with altered expression in tumor cells . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Antibodies to MHC class 1 alleles Kd , Dd , Ld , beta2m and CD 8 molecules all inhibited cytotoxicity . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
We show that , in contrast to other major histocompatibility complex ( MHC ) class 1 molecules that bind CD8alphabeta , TL preferentially binds the homotypic form of CD8alpha ( CD8alphaalpha ) . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
The antitumor effect obtained was mediated predominantly by MHC class 1 antigen restricted CD 8 ( + ) T cells with help from MHC class 2 antigen restricted CD 4 ( + ) T lymphocytes . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Epitope 4 specific CD 8 ( + ) T cells accumulated and persisted in the brains and tumors of SV 11 mice , as determined by analysis with epitope specific MHC class 1 tetramers . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
MHC restricted and unrestricted CD 8 T cells : an evolutionary perspective . ^^^ Injection of adult frogs with anti Xenopus CD 8 monoclonal antibody effects transient CD 8 T cell depletion in vivo that correlates with delayed rejection of MHC disparate skin allografts and an impaired immune response against transplanted syngeneic MHC class 1 negative tumors . ^^^ Our data also suggest that , at least in Xenopus , T cells that express a CD 8 epitope may be effectors in MHC unrestricted anti tumor responses . . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
CD 8 ( + ) T cells are known to down regulate the TCR complex upon ligation with its cognate MHC class 1 peptide complex . ^^^ Using TCR transgenic mice , we found that the down regulation was associated with a decreased affinity of CD 8 ( + ) T cells to MHC class 1 peptide complexes , as determined by MHC class 1 tetramer staining . ^^^ Taken together , these data suggest that the down regulation of TCR components and a subsequent decrease in affinity towards MHC class 1 peptide complexes may be a mechanism by which TCR dependent proliferation of non specifically activated CD 8 ( + ) T cells is avoided . . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
These findings suggest that the large intestine is a site in which unconventional NKT cells carrying the CD 8 ( + ) phenotype ( or DN CD 4 ( ) 8 ( ) ) are abundant and that these cells are independent of MHC and MHC like antigens . . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
A 18 TCR transgenic thymocytes which are H 2E ( k ) restricted and normally selected into the CD 4 lineage , are exclusively selected into the CD 8 lineage in an H 2 ( q ) MHC background . ^^^ Analogues of the cognate peptide for A 18 with antagonist properties for the A 18 TCR have previously been shown to effect a lineage diversion from CD 4 to CD 8 in fetal thymic organ cultures and intriguingly the MHC ( q ) background contains unidentified natural MHC class 2 ligands which similarly show antagonist properties for the A 18 TCR . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Bacterial antigens recognized by CD 8 ( + ) T cells in the context of MHC class 1 are thought to play a crucial role in protection against pathogenic intracellular bacteria . ^^^ Collectively , these findings describe high affinity HLA class 1 binding epitopes that are naturally processed and are recognized efficiently by MHC class 1 restricted CD 8 ( + ) T cells , providing a rational basis for the development of subunit vaccine strategies against tuberculosis and other intracellular infectious diseases . . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Role of phosphoinositide 3 kinase in TCR signaled regulation of CD 8 mediated adhesion to class 1 MHC protein . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
BACKGROUND : Binding of fluorochrome conjugated MHC class 1 tetramers is a powerful means to detect antigen specific CD 8 T lymphocytes . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Consistent with this concept , studies with syngeneic knockout mice showed that MHC class 1 molecules on DCs as well as CD 8 ( + ) T cells were necessary for the antitumor effect of intratumor AdTNF alpha + DCs . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
In the present study we analyzed the contributions of APC , CD 4 and CD 8 T cells , and MHC molecules in the difference between LEW and BN rats to develop a type 1 immune response . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Although none of the monkeys were protected from infection , most demonstrated an anamnestic CTL response with epitope specific CTL precursor frequencies reaching as high as 1 in 20 total PBMC as measured by limiting dilution CTL assay or 25 % of all CD 8 ( + ) T cells using tetrameric MHC I / peptide complexes . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Pulsing of DC with AAV / antigen ( Ag ) gene was found to be superior to pulsing with protein in six different assay systems : ( 1 ) the level of antigen transfer into DC as determined by intracellular staining ; ( 2 ) the level of MHC class 1 restricted killing in cytotoxic T lymphocyte ( CTL ) assays ; ( 3 ) the level of IFN gamma expression ; ( 4 ) the level of DC T cell priming clusters generated ; ( 5 ) the level of CD 80 and CD 83 expression on DC ; and ( 6 ) in the resulting CD 8 : CD 4 ratio . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Two recent papers in Cell ( Moody et al . , 2001 ) and Immunity ( Daniels et al . , 2001 ) describe a novel role for glycosylation in the thymus regulating the interaction of MHC class 1 molecules with CD 8 during thymocyte maturation . . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
METHODS : Samples of tumors growing in the brain or subcutaneously in rats were obtained for 7 consecutive days and were examined immunohistochemically for MHC Class 1 & 2 molecules , and for CD 4 and CD 8 lymphocyte markers . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
MHC class 1 tetramers containing peptide epitopes are sensitive tools for detecting antigen specific CD 8 ( + ) T cell responses . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
This peptide , amino acids 159 167 of PDC E 2 , induces specific MHC class 1 restricted CD 8 ( + ) CTL lines from 10 / 12 HLA A 2 ( + ) PBC patients , but not controls , after in vitro stimulation with antigen pulsed dendritic cells ( DCs ) . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Genetic experiments indicate similarity between binding sites on MHC class 1 ( MHCI ) for CD 8 and on MHCII for CD 4 , but the crystal structures of CD8 / MHCI and CD4 / MHCII complexes suggest critical differences between the interfaces in the two complexes . ^^^ Experiments with soluble multimeric MHC ligands suggest that CD 4 and CD 8 may differ in the mechanisms by which they promote the formation of ternary TCR / MHC / co receptor complexes . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
It is generally accepted that as the result of positive thymic selection , CD 8 expressing T cells recognize peptide antigens presented in the context of MHC class 1 molecules and CD 4 expressing T cells interact with peptide antigens presented by MHC class 2 molecules . ^^^ Here we report the generation of TCRalpha / beta ( + ) , CD 3 ( + ) , CD 4 ( + ) , CD 8 ( ) , MHC class 1 restricted alloreactive T cell clones which were induced using peripheral blood mononuclear cells from healthy individuals following in vitro stimulation with transporter associated with antigen processing ( TAP ) deficient cell lines T 2 . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
To facilitate a detailed investigation of cetacean lymphoid organs , 13 canine , six bovine , one equine , one human and four killer whale specific monoclonal antibodies ( mAbs ) directed against cell surface antigens of the haematopoietic system ( including CD 2 , CD 4 , CD 8 , CD45R , MHC class 2 , granulocyte , thrombocyte , pan T cell and B cell antigen ) , as well as a mAb and a polyclonal antibody ( pAb ) directed against the peptide of the human CD 3 complex , were tested for immunohistochemical cross reactivity on frozen or formalin fixed , paraffin wax embedded lymphatic tissues of harbour porpoises . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Draining cells were Ia ( b+ ) , CD 45 ( + ) , and CD11c ( + ) , and examination of ungrafted corneas revealed numerous similarly CD 45 ( + ) CD11c ( + ) CD 3 ( ) CD8alpha ( ) cells that uniformly lacked major histocompatibility complex ( MHC ) class 2 expression ; transmission electron microscopy confirmed the presence of morphologically similar cells . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
We describe 15 mer peptide P 8 : F 92 106 from the F protein of respiratory syncytial virus ( RSV ) that can act as an MHC class 1 restricted ( H 2K ( d ) ) epitope for RSV specific CD 8 ( + ) CTL . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Splenic lymphocytes from old mice exhibited significantly lower RSV specific MHC class 1 restricted CD 8 ( + ) CTL responses ( P < 0 . 01 0001 ) , and reduced IFN production ( P < 0 . 03 ) than young mice . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
These chimeric tubules were recognized by MHC class 1 restricted T cell hybridoma in vitro and induced in vivo strong CD 8 ( + ) class 1 restricted CTL responses in immunized mice . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Minor histocompatibility antigen specific MHC restricted CD 8 T cell responses elicited by heat shock proteins . ^^^ In mammals , the heat shock proteins ( HSP ) gp 96 and hsp 70 elicit potent specific MHC class 1 restricted CD 8 ( + ) T cell ( CTL ) response to exogenous peptides they chaperone . ^^^ We show in this study that in the adult frog Xenopus , a species whose common ancestors with mammals date back 300 million years , both hsp 70 and gp 96 generate an adaptive specific cellular immune response against chaperoned minor histocompatibility antigenic peptides that effects an accelerated rejection of minor histocompatibility locus disparate skin grafts in vivo and an MHC specific CD 8 ( + ) cytotoxic T cell response in vitro . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
The further in vitro analysis of the generation of CD 8 T cell epitopes revealed a peptide specific effect of IFN gamma on MHC class 1 restricted Ag presentation . ^^^ These results show that despite this modulation of the Ag presentation pattern of CD 8 T cell epitopes , IFN gamma is not generally required for the MHC class 1 and MHC class 2 restricted presentation of L . monocytogenes derived antigenic peptides by professional APCs in vivo . . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
CD 8 binding to MHC class 1 molecules is influenced by T cell maturation and glycosylation . ^^^ CD 8 serves both as an adhesion molecule for class 1 MHC molecules and as a coreceptor with the TCR for T cell activation . ^^^ Here we study the developmental regulation of CD 8 mediated binding to noncognate peptide / MHC ligands ( i . e . , those not bound by the TCR ) . ^^^ We show that CD 8 ' s ability to bind soluble class 1 MHC tetramers and to mediate T cell adhesion under shear flow conditions diminishes as double positive thymocytes mature into CD 8 ( + ) T cells . ^^^ These data suggest that CD 8 ' s ability to interact with class 1 MHC is not fixed and is developmentally regulated through the T cell ' s glycosylation state . . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Two recent papers have shown that this glycosylation changes the avidity of the interaction between CD 8 and MHC class 1 proteins , potentially altering signalling thresholds in thymocyte differentiation . . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Like these gene chips , major histocompatibility complex ( MHC ) class 1 molecules display a large array of peptides on the cell surface for probing by the CD 8 ( + ) T cell repertoire . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
In 94 % of allografts , a curved clinical epithelial rejection line was observed in which ADPase ( + ) / MHC class 2 ( + ) , CD 4 ( + ) , or CD 8 ( + ) T cells were identified . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Immunohistochemical stainings were carried out on these wholemounts using monoclonal antibodies to CD 3 ( T cell ) , CD 4 [ T helper ( Th ) cell ] , CD 8 [ T suppressor ( Ts ) cell ] , macrophages , dendritic cells , B lymphocytes , major histocompatibility complex ( MHC ) class 2 antigen and transforming growth factor beta ( TGF beta ) . ^^^ A massive influx of CD 3 , CD 4 , CD 8 positive cells , macrophages , dendritic cells , MHC class 2 and TGF beta positive cells not only into cornea but also into the iris was observed 7 days after allograft corneal transplantation . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
As a paradigm , we analyzed the reactivity of a Melan A / MART 1 specific and HLA A 2 restricted CD 8 ( + ) T cell clone to either soluble or solid phase presented peptides , including the naturally processed and presented Melan A / MART 1 peptide AAGIGILTV or the peptide analog ELAGIGILTV presented either by the HLA A 2 wild type ( wt ) or mutant ( alanineright arrowvaline aa 245 ) MHC class 1 molecule , which reduces engagement of the CD 8 molecule with the HLA A 2 heavy chain . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
A non class 1 MHC intestinal epithelial surface glycoprotein , gp 180 , binds to CD 8 . ^^^ Furthermore , the sites used for binding by gp 180 are distinct from those used by the conventional CD 8 ligand , class 1 MHC . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Using this model , we have shown that the choice of positively selected ab T cells between the CD 4 helper and CD 8 cytotoxic lineages is regulated by the TCR signaling duration in response to self peptides bound to the MHC . . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Peptide affinity for MHC influences the phenotype of CD 8 ( + ) T cells primed in vivo . ^^^ These data suggest that MHC binding affinity of peptides can regulate the phenotype of the resulting CD 8 ( + ) T cells . . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
To investigate the role of MHC class 1 restricted CD 8 ( + ) T cells in host defense to M . tuberculosis , peripheral blood mononuclear cells ( PBMC ) from healthy BCG vaccinated donors and untreated pulmonary tuberculosis ( TB ) patients in The Gambia were stimulated for 6 days with M . bovis BCG or M . tuberculosis and the CD 8 ( + ) T cell response analyzed . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
The CD 8 coreceptor of cytotoxic T lymphocytes binds to a conserved region of major histocompatibility complex class 1 molecules during recognition of peptide major histocompatibility complex ( MHC ) class 1 antigens on the surface of target cells . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
To analyze inflammatory infiltrates , antibodies against lymphocyte subpopulations ( CD 3 , CD 20 , CD 4 , CD 8 ) , macrophage and activated microglia ( CD 68 ) , major histocompatibility complex ( MHC ) classes 1 and 2 ( HLA ABC and HLA DR ) , and the intercellular adhesion molecules ( ICAM ) 1 and 3 were used . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
The murine CD 8 glycoprotein interacts with both classical MHC class 1 molecules and some nonclassical molecules , including the thymic leukemia Ag ( TL ) . ^^^ Mutations in three CD 8 residues located on the complementarity determining region like loops contacting the negatively charged loop in the alpha 3 domain of MHC class 1 greatly reduced binding to both tetramers . ^^^ Because TL and H 2K ( b ) class 1 sequences are highly conserved in the alpha 3 domain of MHC class 1 , this suggests that CD 8 contacts the alpha 3 domain of TL and H 2K ( b ) in a similar manner . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Local infiltrating cell types , i . e . lymphocyte infiltrates ( CD 4 , CD 8 , CD 20 , CD 3 , interleukin 2 receptor positive , natural killer , gammadelta ) , macrophages and dendritic cells , adhesion and costimulatory molecules ( ICAM 1 and B 7 BB1 ) and major histocompatibility complex ( MHC ) class 1 and class 2 antigens were assessed using semi quantitative immunohistochemical analysis . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
A significant increase in the number of CD 8 ( + ) blasts expressing MHC ( major histocompatibility ) 2 was also observed in the BAL of infected calves . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
With respect to CD 8 ( + ) T cells , we discuss data suggesting that some clones are more dependent upon the presence of MHC class 1 for survival than others . ^^^ Although the proportion of CD 8 ( + ) cells in this MHC dependent state is not known , the corresponding fraction among CD 4 ( + ) T cells seems to be rather small . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Relative cytokine values were related to the degree of CDV infection , MHC class 2 expression and infiltration of CD 4 , CD 8 and CD3epsilon positive lymphocytes . ^^^ In early CDV infection of the cerebellum , either no detectable lesions or mild infiltration of CD 8 positive cells or demyelination and up regulation of MHC class 2 antigen were observed . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
GRP 94 ( gp 96 ) elicits CD 8 ( + ) T cell responses against its bound peptides , a process requiring access of its associated peptides into the MHC class 1 cross presentation pathway of APCs . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
These MHC class 1 peptide complexes form ligands for CD 8 positive T cells and NK cells . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Further , the engagement of CD 160 receptors on normal clonal T lymphocyte populations lacking CD 4 , CD 8 and CD 28 molecules by MHC class 1 molecules results in an increased CD 3 induced cell proliferation . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
To examine the contribution of direct priming in the induction of 10 specific T ( CD 8 ) ( + ) , we generated recombinant vaccinia viruses that express human CMV proteins ( US 2 and US 11 ) that induce the destruction of newly synthesized MHC class 1 molecules . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Using an adoptive transfer system with IL 2 or IL 2R deficient TCR transgenic CD 8 T cells and MHC class 1 tetramers , we demonstrated that the expansion of antiviral CD 8 T cells in secondary lymphoid tissues was IL 2 independent , whereas IL 2 played a more significant role in supporting the continued expansion of these cells within nonlymphoid tissues . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
CD 36 or alphavbeta 3 and alphavbeta 5 integrins are not essential for MHC class 1 cross presentation of cell associated antigen by CD 8 alpha+ murine dendritic cells . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
To demonstrate that transduced antigen can be presented by DCs on MHC class 1 , we chose the lymphocytic choriomeningitis virus glycoprotein ( gp ) as a model antigen , inasmuch as it is recognized by CD 8 T cells from transgenic mice expressing an MHC class 1 restricted T cell receptor specific for the epitope of positions 33 through 41 of gp . ^^^ CONCLUSIONS : These results demonstrate that CD 8 T cells can recognize MHC class 1 epitopes processed from antigen in DCs transduced with lentiviral vectors . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
For maximal CD 8 cell efficacy , CD 4 ( + ) helper T cells responding to major histocompatibility complex ( MHC ) class 2 binding epitopes are required . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
The eyes and eyelids of infected and control mice were processed for immunohistochemistry and double stained for viral antigens and one of the following cell surface markers ( Gr 1 , F4 / 80 , CD 4 , CD 8 , CD45R or MHC class 2 ) or one of the following cytokines ( IL 2 , IL 4 , IL 6 , IL 10 , IL 12 or IFN gamma ) . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
In this study we demonstrate that a disarmed version of the cytotoxin ricin can deliver exogenous CD 8 ( + ) T cell epitopes into the MHC class 1 restricted pathway by a TAP independent , signal peptidase dependent pathway . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
CD 8 ( + ) and CD 4 ( + ) T lymphocytes are important in controlling human CMV ( HCMV ) infection , but the virus has evolved protean mechanisms to inhibit MHC based Ag presentation and escape T lymphocyte immunosurveillance . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Experiments performed with beta ( 2 ) m ( / ) CD 8 ( / ) double knockout mice grafted with a beta ( 2 ) m ( + / + ) MHC class 1 deficient skin showed that sensitized CD 4 T cells directed at beta ( 2 ) m peptides MHC class 2 complexes are sufficient to trigger rapid rejection . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
During allograft rejection or after infusion of interferon gamma they up regulate class 2 MHC molecules , CD 161 ( NKR P1A ) , CD62L and CD 8 , while CD 4 and CD 43 are down modulated . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Cytotoxic T lymphocytes ( CTLs ) with a CD 8 ( + ) phenotype have the potential to recognize and attack major histocompatibility complex ( MHC ) class 1 expressing brain cells . ^^^ Most brain cells , including neurons , can be stimulated to present peptides to CD 8 ( + ) CTLs by MHC class 1 molecules , and are susceptible to CTL mediated cytotoxicity in culture . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
The assembly of functional beta ( 2 ) microglobulin free MHC class 1 molecules that interact with peptides and CD 8 ( + ) T lymphocytes . ^^^ Functional MHC class 1 molecules are expressed on the cell surface in the absence of beta ( 2 ) microglobulin ( beta ( 2 ) m ) light chain that can interact with CD 8 ( + ) T lymphocytes . ^^^ Taken together our data reveal that functional MHC class 1 molecules assemble in the absence of beta ( 2 ) m with peptides and form CD 8 ( + ) T lymphocyte epitopes . . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
The present study shows that in the OM ( + ) LN : 1 ) MHC class 1 expression strictly on hemopoietic cells is sufficient to support the development of a diversified repertoire of CD 8 T cells ; 2 ) the efficiency of positive selection of specific TCR transgenic T cells is not the same as in the thymus ; 3 ) negative selection is very effective , despite the lack of an organized thymic like medulla . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Two APO2L / TRAIL sensitive tumor cell lines ( MHC class 1 ( + ) / II ( + ) or 1 ( + ) / II ( ) ) were selected and specific CD 4 ( + ) HLA DR or CD 8 ( + ) HLA A 2 restricted CTL clones were respectively isolated from autologous tumor infiltrating lymphocytes . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Transgenic expression of the viral capsid genes resulted in inactivation of virus specific CD 8 ( + ) T lymphocytes ( class 1 MHC immune function ) directed against the relevant peptides , but it did not affect production of virus capsid specific antibodies or lymphocyte proliferation to the virus antigen ( class 2 MHC immune functions ) . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
The immunophenotype of rhFL mobilized prostatic cells was consistent with that of Langerhans cells ( MHC class II+ , CD11c+ , CD11b+ , DEC 205+ , CD 8 alpha ) . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Immature CD 4 ( + ) CD 8 ( + ) thymocytes formed a decentralized synapse with multiple foci of MHC accumulation corresponding to areas of exclusion of ICAM 1 . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
The setpoint of viral RNA concentration ( viral load [ VL ] ) during chronic human immunodeficiency virus type 1 ( HIV 1 ) infection reflects a virus host equilibration closely related to CD 8 ( + ) cytotoxic T lymphocyte ( CTL ) responses , which rely heavily on antigen presentation by the human major histocompatibility complex ( MHC ) ( i . e . , HLA ) class 1 molecules . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Positive selection of MHC class Ib restricted CD 8 ( + ) T cells on hematopoietic cells . ^^^ Unlike conventional CD 8 ( + ) T cells , major histocompatibility complex ( MHC ) class Ib restricted CD 8 ( + ) T cells show an activated phenotype in uninfected mice and respond rapidly to foreign invaders . ^^^ We show here that the activated phenotype of MHC class Ib restricted CD 8 ( + ) T cells was partially acquired as a result of interactions in the thymus and reflected an increased capacity to be selected via interactions with MHC molecules on hematopoietic cells . ^^^ Using bone marrow chimeric mice , we have shown that MHC class Ib restricted , but not MHC class Ia restricted , CD 8 ( + ) T cells specific for Listeria monocytogenes were efficiently selected when MHC class 1 was expressed only on hematopoietic cells . ^^^ Thus , the distinct functional properties of MHC class Ib restricted versus MHC class Ia restricted CD 8 ( + ) T cells may result , at least in part , from the different ways in which they are positively selected in the thymus . . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Immunohistochemistry was performed on frozen sections to characterize and quantify T cell subtypes ( CD 3 , CD 4 , and CD 8 ) and markers of immune activation ( major histocompatibility complex [ MHC ] class 2 : HLA DR , HLA DQ ) and inflammation ( intercellular adhesion molecule [ ICAM ] 1 ) . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
We compared development of TCR transgenic CD 4 ( + ) CD 8 ( ) thymocytes in the presence or absence of MHC class 2 autoantigen that does not induce deletion of thymocytes . ^^^ Contrary to the expected negative effect of the presence of autoantigen , approximately 100 % more CD 4 ( + ) CD 8 ( ) thymocytes were found in the presence of MHC class 2 autoantigen than in the neutral background . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
The enhanced development of CD 8 SP thymocytes is dependent on either class 1 or 2 MHC . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Eighteen H 2 ( b ) binding peptides representing sequences from 12 Cpn Ags sensitized target cells for MHC class 1 restricted lysis by CD 8 ( + ) CTL generated from the spleens and lungs of infected mice . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
We demonstrate in this study that increased MHC 1 up regulation on thymic epithelial cells and double positive CD 3 ( / int ) CD 4 ( + ) CD 8 ( + ) thymocytes correlates with the generation of mature single positive CD 4 ( ) CD 8 ( + ) thymocytes that have low expression of CD 8 . ^^^ Treatment of HIV 1 infected thymus with highly active antiretroviral therapy normalizes MHC 1 expression and surface CD 8 expression on such CD 4 ( ) CD 8 ( + ) thymocytes . ^^^ These data indicate that IFN alpha mediated MHC 1 up regulation on thymic epithelial cells may lead to high avidity interactions with developing double positive thymocytes and drive the selection of dysfunctional CD 3 ( + ) CD 8 ( low ) T cells . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Multiple proteases process viral antigens for presentation by MHC class 1 molecules to CD 8 ( + ) T lymphocytes . ^^^ Recognition by CD 8 ( + ) cytotoxic T lymphocytes of any intracellular viral protein requires its initial cytosolic proteolytic processing , the translocation of processed peptides to the endoplasmic reticulum via the transporters associated with antigen processing , and their binding to nascent major histocompatibility complex ( MHC ) class 1 molecules that then present the antigenic peptides at the infected cell surface . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
This study describes a CD 8 ( + ) T cell line specific for a MHC class 1 restricted carcinoembryonic antigen ( CEA ) epitope , residues 526 533 , isolated from CEA transgenic ( CEA . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Using these culture conditions , a marked increase in CD 8 ( + ) CTLs was observed in both donors exhibiting a strong MHC class 1 restricted cytotoxic activity against the host tumor without cross reactivity against nonmalignant host cells . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
The cytolytic activity of effector cells from treated animals was shown to be tumor specific and was mainly CD 8 and MHC Class 1 ( p < 0 . 01 ) restricted . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Class 1 MHC peptide oligomers ( MHC tetramers ) have become popular reagents for the detection and characterization of antigen specific CD 8 ( + ) T cells . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
We propose the hypothesis that MHC class 1 driven interactions between donor DCs and host NK cells or CD 8 T cells might result in DC carried signals controlling the dynamics of alloreactive CD 4 T cell priming and polarization . . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
The MHC class 1 restricted CD 8 CTL effector arm of the adaptive immune response is uniquely equipped to recognize tumor cells as foreign and consequently initiates the cascade of events resulting in their destruction . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
The sow endometrium at different stages of the oestrous cycle : studies on the distribution of CD 2 , CD 4 , CD 8 and MHC class 2 expressing cells . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
An important function of DCs is the ability to take up exogenous antigens and cross present them in the context of major histocompatibility complex ( MHC ) class 1 molecules to CD 8 ( + ) T cells . ^^^ We previously demonstrated that , when cell associated ovalbumin ( OVA ) is injected into mice , only the CD 8 ( + ) DC subset cross presents OVA in the context of MHC class 1 . ^^^ These results suggest that although CD 8 ( + ) DCs constitutively cross present exogenous antigens in the context of MHC class 1 molecules , CD 8 ( ) DCs only do so after activation , such as via ligation of Fc ( gamma ) Rs . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
This syndrome , termed autologous GVHD , is associated with autoreactive CD 8 ( + ) T cells that recognize major histocompatibility complex ( MHC ) class 2 determinants in association with a peptide from the invariant chain . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
For induction of CD 8 ( + ) T cell responses , the FrC sequence has been engineered to remove potentially competitive MHC class 1 binding epitopes and to improve presentation of tumor epitopes . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Trypanosoma cruzi down regulates lipopolysaccharide induced MHC class 1 on human dendritic cells and impairs antigen presentation to specific CD 8 ( + ) T lymphocytes . ^^^ This dysfunction appears to be linked essentially to reduced MHC class 1 molecule expression since the stimulation of the RT ( 476 484 ) peptide specific CD 8 ( + ) T cell line was shown to depend mainly on the MHC class 1 TCR interaction and not on the co stimulatory signals which , however , were also inhibited by T . cruzi . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
We now measure the frequency of p199RY specific CD 8 ( + ) T lymphocytes in the peripheral blood of these monkeys with quantitative precision , using MHC class I / peptide tetramer staining and peptide stimulated interferon gamma Elispot assays . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Bystander effects can enhance the autoimmune process if previously sequestered or cryptic antigens are exposed to the immune system , and superantigens that are produced by the pathogen and are not MHC restricted can result in marked polyclonal activation of CD 4 and CD 8 T cells . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
This contact can be mediated either by the TCR on CD 8 ( + ) T cells that recognize constitutively expressed or acquired MHC molecules on DN T cells , or by the TCR on DN T cells that recognize constitutively expressed MHC molecules on CD 8 ( + ) T cells . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
The ability of killer T cells carrying the CD 8 antigen to detect tumours or intracellular pathogens requires an extensive display of antigenic peptides by major histocompatibility complex ( MHC ) class 1 molecules on the surface of potential target cells . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Thus , classic MHC class 1 Ag presentation and cross priming contribute differentially to the induction of CD 8 ( + ) CTLs following exposure to vaccinia virus via different routes . . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
We have here compared oligomeric and monomeric L ( d ) and K ( b ) peptide MHC complexes and free peptide as stimulators of CD 8 ( + ) T cells expressing the 2C TCR . ^^^ We found that the monomers are indeed effective in activating naive and effector CD 8 ( + ) T cells , but through an unexpected mechanism that involves transfer of peptide from soluble monomers to T cell endogenous MHC ( K ( b ) ) molecules . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
These results show that both splenic CD8alpha+ and CD8alpha DC can present exogenous antigens on major histocompatibility complex ( MHC ) class 1 via a proteasome dependent pathway and suggest that the specialized cross presenting function of CD8alpha+ DC is a result of their ability to endocytose dying cells rather than a unique pathway for handling endosomal contents . . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Ag is then presented by MHC class 1 molecules of CD 8 alpha CD11bhigh DC after a TAP dependent , cytosolic processing . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
DCs transduced with tumor antigen were able to be recognized by both a murine CD 8 ( + ) T cell clone and a murine CD 4 ( + ) T cell line in a cytokine release assay , thereby demonstrating presentation of both MHC class 1 and class 2 gp 100 epitopes . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
We show that immunization of mice with 2 10 10 ( 5 ) mature BMDCs coated with multiple MHC class 1 peptides generates a significant Ag specific CD 8 ( + ) T cell response in both the spleen and nonlymphoid organs . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
IFN alphabeta induced expression of MHC class 1 rescued positive selection of CD 4 ( ) CD 8 ( + ) T cells in fetal thymic organ cultures , but not in adult mice . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Ts express the CD 8 ( + ) CD 28 ( ) phenotype , recognize major histocompatibility complex ( MHC ) class 1 antigens , and suppress the up regulation of costimulatory molecules induced by CD 40 ligation of donor antigen presenting cells . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Some studies point to the importance of major histocompatibility complex ( MHC ) class 1 ( MHC 1 ) and CD 8 ( + ) cells as modifiers of iron overload . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Knockout mice experiments demonstrated that the tumor regression produced by this combination therapy depends on both major histocompatibility complex ( MHC ) class 1 antigen presentation of DCs injected into the tumors and CD 8 ( + ) T cells of the treated host . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Although CD8alpha ( ) DCs are present in normal numbers in ICSBP ( / ) animals , up regulation of CD 40 , CD 80 , and major histocompatibility complex ( MHC ) class 2 expression was found to be impaired in this subset after in vivo microbial stimulation . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Stimulation of CD 8 ( + ) T cells is presumably due to cross presentation , as EBV transformed MHC class 1 positive cell lines , which are incapable of antigen processing , stimulated live influenza virus reactive CD 8 ( + ) T cell lines when loaded with NP derived immunodominant peptides but not following loading with the whole NP molecule . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Effects on the densities of MHC class 2 ( + ) , CD 4 ( + ) and CD 8 ( + ) cells in the mammary gland . ^^^ The aim of this study was to determine the density of MHC class 2 , CD 4 and CD 8 positive cells in mammary glands of sows around parturition , and whether the densities were altered following intramammary inoculation with Escherichia coli prior to parturition . ^^^ There were no changes in density of MHC class 2 , CD 4 and CD 8 positive cells in non inoculated glands around parturition , while significant changes in densities were shown in inoculated glands . ^^^ Here , the density of MHC class 2 , CD 4 and CD 8 positive cells reached a peak at 72 h post inoculation ( p < 0 . 01 ) . ^^^ It is concluded that the density of MHC class 2 , CD 4 and CD 8 positive cells seems to be unaltered around parturition . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Paired terminal ileum and colonic mucosal biopsies from 6 patients with LC , 4 with CC , 5 with CD , 5 with UC , and 10 normal controls were also immunohistochemically stained with monoclonal antibodies to CD 3 , CD 8 , CD 20 , and a class 2 MHC antigen ( LN 3 HLA DR ) . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
In contrast to the IFN gamma knock out mice , both beta 2 microglobulin knock out C57BL / 6 mice , which do not express conventional MHC class 1 molecules and thus can not present antigen to CD 8 T cells , or perforin knock out C57BL / 6 mice , which have no T cell cytotoxic activity , controlled and cleared the infection as well as normal C57BL / 6 mice . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
These results demonstrate that the kinetics of the CD 8 T cell response are similar regardless of whether Ag is accessible to the endogenous MHC class 1 pathway or can only be presented through cross presentation . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Similar experiments using host mice harboring targeted mutations in CD 4 and CD 8 , as well as BMDCs from mice lacking MHC class 1 , MHC class 2 , or IL 12 revealed that this tumor immunity was dependent on the presence of CD4+ and CD8+ cells in the tumor bearing host , as well as MHC class 1 , MHC class 2 , and IL 12 expression by the administered BMDCs . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Mouse MHC class 1 tetramers that are unable to bind to CD 8 reveal the need for CD 8 engagement in order to activate naive CD 8 T cells . ^^^ To address the role of CD8 / MHC 1 interactions , we generated tetramers composed of H 2 K ( b ) molecules with mutations in the alpha 3 domain of H 2 K ( b ) that abolish CD 8 binding . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
We show that this peptide is remarkably immunodominant in that it competes effectively with MHC alloantigens , is efficiently crosspresented by host antigen presenting cells ( APCs ) , and readily elicits naive CD 8 T cell responses in vitro . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Analyses for CD 4 , CD 8 , and other inflammatory cells , plus cytotoxic T cell activity and MHC class 1 specific alloantibody production , did not reveal significant differences in CIITA allograft tissues . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Additional studies allowed us to show that these virus specific IFNgamma ( + ) responding cells detected in this ELISPOT assay were MHC restricted and comprised in the CD8alpha ( bright ) pig T cell subset . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
As a resident of early endosomal phagosomes , Mycobacterium tuberculosis is connected to the iron uptake system of the host macrophage . beta 2 microglobulin ( beta2m ) knockout ( KO ) mice are more susceptible to tuberculosis than wild type mice , which is generally taken as a proof for the role of major histocompatibility complex class 1 ( MHC 1 ) restricted CD 8 T cells in protection against M . tuberculosis . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Stimulation of CD11c ( + ) B 220 ( + ) pDCs with oligodeoxynucleotides containing certain CpG motifs plus CD 40 ligand plus GM CSF led to increased MHC class 2 , CD 80 , CD 86 , and CD8alpha expression levels , to a switch in chemokine receptor expression that affected their migration , to IFN alpha and IL 12 secretion , and to the acquisition of priming capacities for both CD 4 ( + ) and CD 8 ( + ) OVA specific TCR transgenic naive T cells . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Ag expressed exclusively in the anterior pituitary gland and secreted locally by pituitary somatotrophs can gain access to the MHC class 1 presentation pathway and activate CD 8 T cells . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
We demonstrate here that sequential delivery of these mutant epitopes provokes original antigenic sin in CD 8 T cells as demonstrated by attenuation of CTLs , intracellular IFN gamma production , and MHC 1 peptide tetramer staining . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
In the MHC class 2 disparate mice , the vIL 10 Tg heart grafts showed severe CD 8 T cell infiltration and increased interferon ( IFN ) gamma mRNA expression compared with non Tg grafts . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
High affinity xenoreactive TCR : MHC interaction recruits CD 8 in absence of binding to MHC . ^^^ Yet , T cell binding and cytolytic activity are independent of CD 8 when stimulated with human p1049 / A2 as demonstrated with anti CD 8 Abs that block CD 8 association with MHC . ^^^ Confocal fluorescence microscopy and fluorescence resonance energy transfer flow cytometry demonstrate CD 8 is recruited to the site of TCR : peptide MHC binding . ^^^ Taken together , these results indicate that there exists another mechanism for recruitment of CD 8 during high affinity TCR : peptide MHC engagement . . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
In the NOD mouse model of type 1 diabetes , major histocompatibilitycomplex ( MHC ) class 1 restricted CD 8 ( + ) T cells are essential for disease development . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
The spleen and lymph nodes ( LN ) of relB ( / ) BMC contain a small number of residual DC , mainly CD8alpha ( + ) , that fail to up regulate MHC class 2 and co stimulatory molecules after stimulation in vitro . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
IFNgamma surface capture and MHC class 1 tetramer staining were independently used to isolate EBV specific CD 8 ( + ) T cells from peripheral blood . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
In a cellular immune response , antigenic peptides derived by intracellular processing of foreign pathogens are bound to the class 1 major histocompatability complex ( MHC 1 ) and presented to CD 8 ( + ) cytotoxic T cells . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
CD8alpha ( + ) DC from schistosome infected mice exhibited a 2 to 3 fold increase in the expression of MHC class 2 , CD 80 , and CD 40 ( but not CD 86 ) compared with DC from uninfected control animals , while CD8alpha ( ) DC exhibited a 2 to 3 fold increase in the expression of MHC class 2 and CD 80 and no alteration , compared with DC from uninfected mice , in the expression of CD 86 or CD 40 . ^^^ In contrast , infection with T . gondii resulted in a more pronounced increase in the expression of activation associated molecules ( MHC class 2 , CD 80 , CD 86 , and CD 40 ) on both CD8alpha ( ) and CD8alpha ( + ) splenic DC and promoted elevated IL 12 production by DC . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
In total , these studies demonstrate that CD 8 ( + ) host T cells can effectively resist MHC matched MiHA mismatched donor PCs via alternative effector pathway ( s ) independent of perforin , FasL , TNFR 1 , and TRAIL dependent cytotoxicity . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Allogeneic and syngeneic class 1 MHC complexes drive the association of CD 8 and TCR on 2C T cells . ^^^ In most cases , cytotoxic T cell activation is dependent on the interaction of the T cell receptor ( TCR ) and CD 8 with MHC class 1 molecules . ^^^ In the CD 8 ( + ) T cell system based on the mouse cytotoxic T cell clone 2C , recognition of the allogeneic MHC L ( d ) exhibits a less significant role for CD 8 than recognition of the syngeneic MHC K ( b ) . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Significantly increased percentages of CD 2 ( + ) and CD 8 ( + ) T lymphocytes were also found in peripheral blood of these animals at this time , while the percentages of CD 4 ( + ) and MHC class 2 lymphocytes were significantly reduced . ^^^ The percentages of CD 2 ( + ) , CD 8 ( + ) and MHC class 2 lymphocytes in peripheral blood were also significantly increased at this time ; the percentage of MHC class 2 lymphocytes remained elevated at 15 days pi . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
The ability of CD 91 to internalize alpha defensins and to cross present exogenous antigen to cytotoxic T lymphocytes through major histocompatibility complex ( MHC ) class 1 may maintain CD 8 ( + ) responses in these patients . . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
IL 6 deletion did not affect the humoral response directed against virus , nor did it affect the expression of CD 4 , CD 8 , MHC class 1 , or MHC class 2 in the CNS . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
The generated killer cells were conventional CD 8 ( + ) L ( d ) class 1 MHC molecule restricted cytotoxic T lymphocytes ( CTLs ) and specific for the HCV E 1 structural protein . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Protein antigens encoded by nucleic acids delivered to dendritic cell ( DC ) are produced inside the cell and , thus , can stimulate MHC class 1 mediated activation of CD 8 ( + ) T cell immune responses . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
We studied the size and avidity of primary and recall CD 8 ( + ) T cell responses in vivo in mice immunized with dendritic cells presenting different densities of a MHC class 1 restricted peptide . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
We monitored the frequencies and kinetics of beta gal specific CD 8 T cells in infected mice by MHC class 1 tetramer staining . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
The susceptibility of K ( b / ) D ( b / ) mice can not be explained by the failure of CD 8 ( + ) T cells ( presumably MHC class Ib restricted ) to respond to the infection . ^^^ Thus , neither the presence of MHC class Ib molecules themselves , nor the activity of a population of nonclassical CD 8 ( + ) effector cells , fully restored the deficit caused by the absence of MHC class Ia molecules , suggesting a unique role for MHC class Ia molecules in protective immunity against M . tuberculosis . . ^^^ A similar pattern of granuloma formation has previously been observed in other types of MHC class 1 deficient mice , but not CD8alpha ( / ) mice . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
In CD 4 T cell depleted , CD 4 ( / ) , and MHC class 2 ( / ) mice , L . monocytogenes infection induced CD 8 T cell activation and primed epitope specific CD 8 T cells to levels commensurate with those in normal C57BL / 6 mice . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
These tetraparental mice showed primary protective CD 8 ( + ) T cell responses , after lymphocytic choriomeningitis virus infection , that were peptide specifically restricted to either thymic or nonthymic epithelial MHC at comparable levels . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Influence of pre ovulatory insemination and early pregnancy on the distribution of CD 2 , CD 4 , CD 8 and MHC class 2 expressing cells in the sow endometrium . ^^^ The aim was to investigate the distribution of CD 2 ( + ) , CD 4 ( + ) and CD 8 ( + ) lymphocyte subpopulations and MHC class 2 expressing cells in the sow endometrium following pre ovulatory insemination and during early pregnancy . ^^^ In conclusion , the immunomodulation illustrated by the distribution of CD 2 ( + ) , CD 4 ( + ) , CD 8 ( + ) and MHC class 2 expressing cells at the attachment sites on day 19 , i . e . the low numbers in the surface epithelium and the high numbers in the subepithelial layer , shows that the porcine trophoblast may influence the endometrium to develop the conditions required for embryonic attachment and survival in pigs . . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
The separated PBL fractions were characterized by RT PCR analysis using specific primers for the amplification of trout IgM heavy chain constant region ( CH 1 ) , T cell receptor alpha chain ( TCRalpha ) , CD8alpha and major histocompatibility complex ( MHC ) class 1 gene fragments . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
LPS induced IFN gamma production by CD 8 ( + ) T cells was MHC class 1 independent and was restricted to CD 44 ( high ) ( memory phenotype ) cells . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Phenotypic analysis of MHC class I / peptide tetramer positive cells revealed that HLA A 2 restricted CMV specific CD 8 T cells exhibit the lowest percentage of CCR 7 ( + ) cells ( 0 . 5 5 % ) , while HLA A 2 restricted flu and HLA B 8 restricted EBV specific CD 8 T cells showed the highest ( 45 70 % ) . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
A class of DC , uniquely found in s . c . lymph nodes , expressed the highest level of Fc gamma RI and also high levels of MHC class 2 , DEC 205 , CD 40 , and CD 86 , with a low level of CD 8 alpha , corresponding to the phenotype for Langerhans derived DC , which are highly active in Ag processing . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Surface expression of CD 4 or CD 8 is commonly used to identify T cell subsets that recognize antigen presented by class 2 MHC or class 1 MHC , respectively . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
IVIG does not contain significant amounts of blocking antibodies against CD 3 , CD 4 , CD 8 , CD 20 , CD 14 , CD 40 , MHC class 1 and class 2 . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
T cells harvested from brains and spleens of scrapie infected mice were analyzed using a newly identified mouse PrP ( mPrP ) peptide bearing the canonical binding motifs to major histocompatibility complex ( MHC ) class 1 H 2 ( b ) or H 2 ( d ) molecules , appropriate MHC class 1 tetramers made to include these peptides , and CD 4 and CD 8 T cells stimulated with 15 mer overlapping peptides covering the whole mPrP . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Furthermore , those cells that are positively selected develop into two different lineages , CD 4 or CD 8 , depending on whether their TCRs bind to MHC class 2 or 1 , respectively . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
During the disease course , we have : ( 1 ) examined the expression of a number of inflammatory markers in the CNS , including MHC class 2 , CD 68 , CD11b ( CR 3 ) , 7 / 4 , F4 / 80 , nitrotyrosine , CD 4 and CD 8 ; ( 2 ) profiled cytokine production [ tumour necrosis factor alpha ( TNF alpha ) , transforming growth factor ( TGF beta 1 ) and interleukin 1 beta ( IL 1 beta ) ] ; and ( 3 ) studied blood brain barrier ( BBB ) integrity . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Thus , we hypothesized that islet specific homing may be in part dependent on IS CD 8 ( + ) cells ' recognition of the cognate major histocompatibility complex ( MHC ) / peptide complexes presented by pancreatic endothelial cells , which acquire the antigen ( insulin ) from beta cells . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Experiments using knockout mice demonstrated that host expression of MHC Class 1 , but not Class 2 , IL 4 , or IL 12 , was necessary for AdMDC to exert its antitumor effect , and immunohistochemistry demonstrated infiltrates of CD 8 and CD 86 cells , but not CD 4 cells in treated tumors . ^^^ These studies highlight a new function for macrophage derived chemokine by demonstrating that it possesses in vivo antitumor activity with CD 8 T cells as the effector cells , and interestingly , that the CD 4 cell / MHC 2 pathway of CD 8 cell activation is not required for the antitumor effects of this chemokine . ( H ) . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Requirement for Q 226 , but not multiple charged residues , in the class 1 MHC CD loop / D strand for TCR activated CD 8 accessory function . ^^^ Activation of CD8+ cytotoxic T lymphocytes typically begins with recognition of class 1 MHC peptide complexes by the TCR and CD 8 as a coreceptor . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Indeed , the TCR alpha subunit restores development of pT alpha deficient thymocytes to the CD 4 ( + ) CD 8 ( + ) stage even in the absence of conventional MHC class 1 and class 2 ligands . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Because the function of HLA B 27 , as a major histocompatibility complex ( MHC ) class 1 molecule , is peptide presentation to CD 8 ( + ) T cells , research has concentrated on the role of HLA B 27 as a restriction element for CD 8 ( + ) cytotoxic T lymphocytes in pathogenesis . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Immunohistochemical labelling and computer assisted morphometric analysis was used to identify expression of MHC class 2 , CD 3 , CD79a , IgG , IgM , IgA , and leucocyte antigen L 1 ( L 1 ) by cells in sections from 19 cats , and expression of CD 4 and CD 8 by cells in sections from 17 cats . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
There were no differences in the percentages of CD 4 ( + ) and CD 8 ( + ) T cells between the groups , but the proportion of mature B cells [ CD 21 ( + ) / major histocompatibility complex ( MHC ) class 2 ( + ) ] was greater in those fed the probiotic . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
When T cell clones were first grown in long term cell culture , each clone was considered to be capable of displaying a limited range of functional activities , constrained by the clones ' coreceptor , CD 4 or CD 8 , and the specificity of its antigen specific receptor ( TCR ) for one or a few peptides in association with a class 1 or class 2 MHC molecule . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Cross reactive LCMV specific CD 8 T cells were directly visualized using LCMV peptide charged MHC tetramers to costain T cells that were stimulated to produce intracellular IFN gamma in response to allogeneic target cells . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
We confirm the similarity of KG 1 to DCs by demonstrating its capacity to present exogenous Ags in an MHC class 1 restricted fashion to CD 8 ( + ) T cell hybridomas , a phenomenon called cross presentation . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
In this study , we report the identification of a MHC class 1 peptide in the neu protein that is recognized by CD 8 ( + ) T cells derived from vaccinated FVB / N mice . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
The advent in recent years of the application of tetrameric arrays of class 1 peptide MHC complexes now enables us to detect and study rare populations of Ag specific CD 8 ( + ) T cells . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
A quantitative immunohistochemical study with monoclonal antibodies against CD 4 , CD 8 , MHC 1 and MHC 2 was carried out in the spleen of these animals . ^^^ Antigenic stimulation produced , in the spleen red pulp of transplanted animals , a significant increase ( P < 0 . 001 ) in the percentage of NOS , CD 8 and MHC 1 immunoreactive cells in relation with values observed in control animals . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
The T lymphocyte surface glycoproteins CD 8 and CD 4 enhance T cell antigen recognition by binding to MHC class 1 and class 2 molecules , respectively . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
MHC class 1 restricted antigen presentation is an essential step in the priming of CD 8 T lymphocytes during immune responses to infection . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
For recognition by CD 8 ( + ) lymphocytes , peptides derived from cytosolically processed antigen need to access MHC class 1 molecules en route to the target cell surface . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
This effect was due to activation of MHC class 1 restricted CD 8 ( + ) T cells coupled with an increased secretion of proinflammatory cytokines IFN gamma , TNF alpha and IL 2 . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
CD 8 ( + ) effector T cells recognize malignant cells by monitoring their surface for the presence of tumor derived peptides bound to MHC class 1 molecules . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Nevertheless , interactions with classical MHC class 2 molecules are required for CD 4 ( + ) T cells to survive in CD 8 ( + ) T cell containing mice . . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Here we show that proinsulin B 24 C36 peptide binds to 1 A ( g 7 ) , the MHC class 2 molecule of the NOD mouse , and , after intranasal administration , induces regulatory CD 4 ( + ) T cells that , in the absence of CD 8 ( + ) T cells , block the adoptive transfer of diabetes . ^^^ We then determined that B 24 C36 , and its core sequence B 25 C34 , bind to K ( d ) , the NOD mouse MHC class 1 molecule , and elicit CD 8 ( + ) CTLs . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
We have studied CD 8 ( + ) T cell mediated brain inflammation by transferring MHC class 1 restricted hemagglutinin ( HA ) reactive T cells from a TCR transgenic mouse line into transgenic mice , which express HA in astrocytes . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Decreased binding of peptides MHC class 1 ( pMHC ) multimeric complexes to CD 8 affects their binding avidity for the TCR but does not significantly impact on pMHC / TCR dissociation rate . ^^^ In this study we used soluble peptides MHC class 1 ( pMHC ) multimeric complexes bearing mutations in the CD 8 binding site that impair their binding to the MHC , together with altered peptide ligands , to assess the impact of CD 8 on pMHC binding to the TCR . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Therefore , fusions of DCs and tumor cells express both MHC class 1 and class 2 restricted tumor associated epitopes and may be useful for the induction of tumor reactive CD 8 ( + ) and CD 4 ( + ) T cells in vitro and in human vaccination trials . . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Moreover , it efficiently colocalizes with the proteosome 20s subunit , which degrades proteins into peptides for presentation to CD 8 T cells by the MHC class 1 pathway . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
In contrast , encapsulation of same antigen in egg phosphatidyl choline ( egg PC ) liposomes , just like antigen incomplete Freund ' s adjuvant ( IFA ) complex , has inefficient access to the cytosolic pathway of MHC 1 dependent antigen presentation and failed to generate antigen specific CD 8 ( + ) cytotoxic T cell response . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
We found no evidence for loss of D ( b ) or K ( b ) class 1 MHC molecules from C3R cells recovered from outgrown tumours , and fluorescent MHC / peptide tetramer staining revealed E 7 gene vaccination did not delete RAHYNIVTF specific CD 8 ( + ) T cells . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
The linkage of gamma tubulin to E 7 targeted antigen to centrosomal compartments , resulted in enhanced MHC class 1 presentation of E 7 , and led to a marked increase in the number of E 7 specific CD 8 ( + ) T cell precursors as well as a potent CD 4 independent antitumor effect against an E 7 expressing tumor cell line , TC 1 . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Although CD 8 T cells have been implicated both early and late in this process , the requirement for direct interaction between these cells and MHC class 1 on the beta cells has not been demonstrated . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Activated CD 8 ( + ) T cells mainly expressed MHC class 2 and some expressed CD 25 . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
MHC class 1 restricted CD 8 ( + ) T cells are a crucial component of the host defense against mycobacterial infection in mice , but it has often proved very difficult to identify the CD 8 T cell response in humans . ^^^ Human group 1 CD 1 molecules ( CD1a , b , c ) mediate MHC independent presentation of mycobacteria derived lipid and glycolipid Ags to CD 8 ( + ) T cells , and their intracellular localization to the endocytic system may favor efficient monitoring of phagosome resident mycobacteria . ^^^ Here , we show that bacillus Calmette Gurin ( BCG ) immunized subjects contain a significant circulating pool of CD 8 ( + ) T cells that recognize BCG infected DCs in a CD 1 dependent , but MHC independent , manner . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
In comparison to wild type Yersinia , an attenuated Y . pseudotuberculosis yopK null mutant strain hypertranslocates chimeric YopE / LLO into the cytosol of macrophages , resulting in enhanced major histocompatibility complex ( MHC ) class 1 restricted antigen presentation of an LLO derived CD 8 T cell epitope . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Whereas interactions between the TCRalpha beta and self MHC : peptide complexes are clearly required for positive selection of mature CD 4 ( + ) and CD 8 ( + ) T cells during intrathymic development , the role of self or foreign ligands in maintaining the peripheral T cell repertoire is still controversial . ^^^ Our data indicate that CD 8 ( + ) T cells in peripheral lymphoid tissues are present in normal numbers in the absence of self MHC class 1 : peptide ligands . ^^^ Indeed TCRalpha beta ( + ) IEL subsets expressing CD8alpha beta or CD8alpha alpha are both dramatically reduced in K 14 beta2m mice , suggesting that the development , survival or expansion of CD 8 ( + ) IEL depends upon interaction of the TCR with MHC class 1 : peptide or other beta2m associated ligands elsewhere than on thymic cortical epithelium . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
This vaccine also induced MHC class 1 antigen restricted CD 8 ( + ) T cell responses . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Alternate class 1 MHC ( MHC 1 ) Ag processing via cytosolic or vacuolar pathways leads to cross presentation of exogenous Ag to CD 8 T cells . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Recent evidence indicates that B cells also present exogenous Ags in the context of MHC class 1 to CD 8 ( + ) T cells and thus may play an important role in the modulation of CTL responses . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
We have produced soluble T cell receptor ( TCR ) derived from a human CD 8 ( + ) cytotoxic T lymphocyte ( CTL ) clone D 3 that recognizes the immunodominant HIV Gag peptide SLYNTVATL ( SL 9 ) in association with major histocompatibility complex ( MHC ) class 1 protein HLA A 2 . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Here , we determined if altering the route of vaccine delivery or incorporation of the target antigen into VLPs of a heterologous papillomavirus type could overcome inhibition of MHC class 1 antigen presentation by neutralizing antibodies , resulting in a boosting of CD 8 ( + ) T cell responses against the incorporated antigen , HPV 16 E7 . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
From this perspective , the appearance of MHC 1 signaled thymocytes as CD4+8lo cells would be due to effects on CD 8 surface protein expression , not CD 8 gene expression . ^^^ But contrary to this perspective , this study demonstrates that MHC 1 signaled thymocytes appear as CD4+8lo cells because of transient down regulation of CD 8 gene expression , not because of changes in CD 8 surface protein expression or distribution . ^^^ This study also demonstrates that initial cessation of CD 8 gene expression in MHC 1 signaled thymocytes is not necessarily indicative of commitment to the CD4+ T cell lineage , as such thymocytes retain the potential to differentiate into CD8+ T cells . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Anti CD40L mAb plus bone marrow transplantation ( BMT ) and recipient CD 8 T cell depletion permits long term mixed hematopoietic chimerism and systemic donor specific tolerance to be achieved across full MHC barriers . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Together these data demonstrate that infusion of single MHC class 1 locus mismatched lymphocytes may induce donor specific transplantation tolerance through activation of DN Treg cells , which can suppress antihost CD 8 ( + ) T cells and prevent the development of GVHD . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
RESULTS : The most common CD 45 ( + ) retinal cells were CD11b ( + ) , F4 / 80 ( + ) , CD 8 alpha ( + ) , CD 80 ( + ) , and major histocompatibility complex ( MHC ) class 2 ( lo ) , a phenotype characteristic of central nervous system ( CNS ) microglia ( MG ) . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Using the R8A variant of CD8alpha , a panel of anti CD8alpha Abs , and three MHC class 1 ( MHCI ) variants differing in key residues known to be involved in CD8alpha interaction , we show that the introduction of the CD8beta stalk leads to a different topology of the CD8alpha MHCI complex without altering the overall structure of the Ig like domain of CD8alpha or causing the MHCI to employ different residues to interact with the CD8alpha Ig domain . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
MHC dependent CD 8 ( + ) T cell responses have been associated with control of viral replication and slower disease progression during lentiviral infections . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Vaccine induced , HIV 1 specific effector activities included IFN gamma secretion and class 1 MHC restricted CD 8 ( + ) CTL . ^^^ Rectal and systemic CD 8 ( + ) CTL clones established in 1 vaccine recipient revealed similar Env specific responses and MHC restriction . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
MHC class 1 and class 2 tetramers can be used to detect autoreactive CD 4 ( + ) and CD 8 ( + ) T cells , while nonclassical MHC ( such as CD1d ) tetramer can be used to detect other T cell groups , for example natural killer T cells . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Human inhibitory receptors Ig like transcript 2 ( ILT 2 ) and ILT 4 compete with CD 8 for MHC class 1 binding and bind preferentially to HLA G . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Mature CD4+ and CD 8 + T lymphocytes develop in the thymus from precursors with diverse clonally distributed receptors , possessing binding sites with negligible , intermediate , or high affinity for selfpeptide major histocompatibility complex ( MHC ) ligands . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
They expressed MHC class 2 , CD11b , CD 8 and weakly CD 4 on their surface . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Because MHC class 2 ( / ) ( class 2 ( / ) ) mice generate efferent Tr cells following a . c . inoculation , we conclude that conventional CD 4 ( + ) T cells are not needed for the development of efferent CD 8 ( + ) T cells . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Alternate class 1 MHC ( MHC 1 ) processing allows macrophages to present Ags from MTB and other bacteria to CD 8 ( + ) T cells , but the effect of PAMPs on this processing pathway is unknown . ^^^ By inhibiting both MHC 2 and alternate MHC 1 Ag processing , pathogens that establish prolonged infection of macrophages ( > 16 h ) , e . g . , MTB , may immunologically silence macrophages and evade surveillance by both CD 4 ( + ) and CD 8 ( + ) T cells , promoting chronic infection . . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
In vivo CD 4 ( + ) and CD 8 ( + ) T cell depleting strategies and genetically altered mice that did not express MHC class 1 or class 2 antigens were used to study the allorecognition and effector pathways of islet allograft rejection in different strains of mice , including autoimmunity prone nonobese diabetic ( NOD ) mice . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
By selecting the CD 45 ( + ) population and simultaneous staining with several leukocyte lineage markers ( CD 3 , CD 4 , CD 8 , CD 56 , and CD 19 ) , MHC mismatched leukocytes were consistently detected in cell suspensions prepared from directly stained whole blood samples with a threshold sensitivity as low as 0 . 1 % 0 . 2 % . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
With increased proportions of nonselectable thymocytes , positive selection of MHC class 2 specific precursors was enhanced , generating not only CD 4 but also CD 8 thymocytes . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
MHC class 1 tetramer staining , intracellular cytokine staining and ELISPOT assays have made it possible to quantify CD 8 ( + ) T cell responses precisely during and following viral and bacterial infection . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Potential role of NKG2D / MHC class 1 related chain A interaction in intrathymic maturation of single positive CD 8 T cells . ^^^ The nonclassical MHC class 1 molecule MHC class 1 related chain A ( MICA ) interacts with the NKG2D receptor expressed at the surface of most peripheral CD 8 T cells , gammadelta T cells , and NK cells . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
We previously demonstrated that protection induced by radiation attenuated ( gamma ) Plasmodium berghei sporozoites is linked to MHC class 1 restricted CD 8 ( + ) T cells specific for exoerythrocytic stage Ags , and that activated intrahepatic memory CD 8 ( + ) T cells are associated with protracted protection . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
The two populations were also measured as a percentage of respective base populations ( CD2+ cells for CD 8 and MHC II+ cells for CD 21 ) . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Coculture experiments of MHC class I / II positive myoblasts with CD 4 and CD 8 T cells in the presence of antigen demonstrated the functional consequences of muscle related B 7 H1 expression : production of inflammatory cytokines , IFN gamma and IL 2 , by CD 4 as well CD 8 T cells was markedly enhanced in the presence of a neutralizing anti B 7 H1 antibody . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Influence of post ovulatory insemination on sperm distribution , pregnancy and the infiltration by cells of the immune system , and the distribution of CD 2 , CD 4 , CD 8 and MHC class 2 expressing cells in the sow endometrium . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
The percentage of CD 4 ( + ) , CD 5 ( + ) , and CD 8 ( + ) cells decreased while the percentage of CD 14 ( + ) , IgM ( + ) , and MHC class 2 ( + ) cells increased significantly after endotoxin infusion . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
This leads to activation of both MHC class 1 restricted CD 8 cells as well as MHC class 2 restricted CD 4 T cells . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Using this system and MHC tetramers to define Ag specific T cells , we demonstrate that contrary to being VLAs , expression of alpha ( 1 ) beta ( 1 ) and alpha ( 2 ) beta ( 1 ) can be rapidly induced on acutely activated T cells , that expression of alpha ( 1 ) beta ( 1 ) remains elevated on memory T cells , and that expression of alpha ( 1 ) beta ( 1 ) parallels that of viral specific effector CD 8 ( + ) T cells ( defined by tetramer and IFN gamma staining ) . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
TCR ligation by the self peptide associated MHC molecules is essential for T cell development in the thymus , so that class 2 MHC deficient mice do not generate CD 4 ( + ) CD 8 ( ) T cells . ^^^ The present results show that the administration of anti TCR mAb into class 2 MHC deficient mice restores the generation of CD 4 ( + ) CD 8 ( ) T cells in vivo . ^^^ Unlike peripheral CD 4 T cells that disappeared within 5 wk after the treatment , CD 4 ( + ) CD 8 ( ) thymocytes remained undiminished even after 5 wk , suggesting that CD 4 T cells in the thymus are maintained separately from circulating CD 4 T cells and even without class 2 MHC molecules . ^^^ It was also found that the mass of medullary region in the thymus , which was reduced in class 2 MHC deficient mice , was restored by the anti TCR administration , suggesting that the medulla for CD 4 ( + ) CD 8 ( ) thymocytes is formed independently of the medulla for CD 4 ( ) CD 8 ( + ) thymocytes . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Since anti Hu antibodies are not pathogenic , and oligoclonal CD 8 ( + ) T cells infiltrate neoplastic and nervous tissues , we examined MHC class 1 restricted immunogenicity of human HuD . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
It has been argued that the importance of cross priming of CD 8 ( + ) CTL responses has been overemphasized , despite strong evidence that these mechanisms operate when infectious organisms , tumors or self antigens can not efficiently access the biosynthetic MHC class 1 processing pathway of DC . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Using adoptive transfer of 2C / recombinase activating gene 2 ( RAG 2 ) ( / ) TCR transgenic T cells , we found that the survival of both naive and effector CD 8 ( + ) T cells was dependent upon host expression of the same MHC class 1 alleles that supported thymic selection . ^^^ Finally , T cell death in the absence of appropriate host MHC class 1 was overcome by elimination of Fas signaling but not by overexpression of Bcl 10 ( L ) by CD 8 ( + ) T cells . ^^^ These results suggest that , in the absence of a survival signal provided by engagement of host MHC / self peptide complexes , CD 8 ( + ) T cells die via a Fas dependent , mitochondria independent pathway . . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Differences in predisposition to HSR according to ethnicity and baseline CD 8 cell count may be explained by the reported MHC genetic associations with HSR . . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
The data enumerate the specific CD 8 ( + ) T cell response to six MHC class 1 restricted epitopes and the specific CD 4 ( + ) T cell responses to two MHC class 2 restricted epitopes . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
MHC class 1 gene conversion mutations alter the CD 8 T cell repertoire . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
To investigate whether CD1d tissue distribution and expression levels confer differential capacity in selecting these two T cell subsets , we analyzed CD 8 and NKT cell compartments in K ( b ) CD1d transgenic mice that lack endogenous MHC class Ia and CD1d , respectively . ^^^ We found that MHC class Ia like expression pattern and tissue distribution are not sufficient for CD1d to rescue the development of CD 8 ( + ) T cells , suggesting that unique structural features of CD1d preclude its active participation in selection of CD 8 ( + ) T cells . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
The majority of the Chlamydia reactive CD 8 ( + ) T cells were non MHC class Ia restricted in all three of the individuals tested . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Here we show that exosomes released from ovalbumin ( OVA ) protein pulsed bone marrow derived dendritic cells ( BM DCs ) weakly present the peptide determinants to OVA specific MHC class 1 restricted CD 8 ( + ) T cell hybridomas . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
They were CD 8 ( + ) / CD28 ( ) and expressed the MHC class 1 binding killer cell Ig like receptor ( KIR ) 3DL2 / p140 , but not KIR3DL1 / p70 , KIR2DL1 / p58 . 1 and KIR2DL2 / 3 / p58 . 2 . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
A correlation between TCR Valpha docking on MHC and CD 8 dependence : implications for T cell selection . ^^^ In light of new information provided by this structure , we have reanalyzed the existing TCR / MHC cocrystal structures and discovered unique features of TCR Valpha domain position on class 1 MHC that correlate with CD 8 dependence . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Natural killer cells activated by MHC class 1 ( low ) targets prime dendritic cells to induce protective CD 8 T cell responses . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
The thymus leukemia antigens ( TL ) belong to the MHC class Ib family and can be recognized by CD 8 dependent or independent cytotoxic T lymphocytes ( CTL ) showing TL , but not H 2 , restriction . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Chimeric beta 2 microglobulin / CD3zeta polypeptides expressed in T cells convert MHC class 1 peptide ligands into T cell activation receptors : a potential tool for specific targeting of pathogenic CD 8 ( + ) T cells . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Nevertheless , CD 8 ( + ) T cells that recognize Chlamydia Ags in the context of MHC class 1 molecules are primed during infection . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
The resulting class 1 MHC deficient CD 8 T cells are functional and upon activation remain susceptible to natural killer cell toxicity in vivo . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Flow cytometric analyses showed that the degree of CD 8 and CD 4 T cell proliferation correlated with the expression levels of class 1 and 2 major histocompatibility complex ( MHC ) antigens , respectively . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
However , the level of expression of CD 4 , CD 8 , class 1 MHC , or class 2 MHC in the central nervous system of IFN gamma ( / ) H 2 ( q ) mice was lower than those in IFN gamma ( + / + ) H 2 ( q ) mice . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Our previous studies showed that the ability of an epitope to induce major histocompatibility complex ( MHC ) class 1 restricted CD 8 ( + ) T cells was , indeed , enhanced by its being attached to the proposed translocatory sequence of the HIV 1 tat protein . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Whereas under physiologic conditions muscle fibers are negative for major histocompatibility complex ( MHC ) class 1 antigens , these are upregulated under pathologic conditions , thus rendering muscle a possible target for the recognition by cytotoxic CD 8 T cells . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
These parasites can deliver antigens to the host cell cytoplasm that are presented through MHC class 1 molecules to protective CD 8 T cells . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Observing high frequencies of CD 4 and CD 8 coreceptor expression in human CD56+ T cells , we examined the potential role of major histocompatibility complex ( MHC ) class 2 molecules in the activation of these cells . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Cutting edge : predetermined avidity of human CD 8 T cells expanded on calibrated MHC / anti CD 28 coated microspheres . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
CD 8 ( + ) DC , but not CD 4 ( + ) or CD 4 ( ) CD 8 ( ) DC , synthesize CyC , which accumulates in MHC 2 ( + ) Lamp ( + ) compartments . ^^^ We then analyzed MHC 2 localization and Ag presentation in CD 8 ( + ) DC , bone marrow derived DC , and spleen derived DC lines , from CyC deficient mice . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Oligodeoxynucleotides ( ODN ) with immune stimulating sequences ( ISS ) containing CpG motifs facilitate the priming of MHC class 1 restricted CD 8 ( + ) T cell responses to proteins or peptides . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
In this study , we performed three color flow cytometric analysis for activation associated cell surface molecules ( CD 2 , CD11a , CD45RA like and major histocompatibility complex antigen class 2 ( MHC 2 ) ) and light scatters ( cellular size and complexity ) to examine whether phenotypic changes also occurred in CD 4 ( + ) PBLs in addition to CD 8 ( + ) PBLs , of five FIV infected cats and one uninfected cat . ^^^ It was shown that ( 1 ) CD8alpha ( + ) PBLs , but not CD 4 ( + ) PBLs , had a distinct subpopulation with increased CD11a expression accompanying a reduced CD8beta chain and increased intracellular granules ( 2 ) CD8alpha ( + ) PBLs , but not CD 4 ( + ) PBLs , expressed CD45RA like antigen with diverse expression levels and ( 3 ) MHC 2 expression was greater in CD8alpha ( + ) PBLs than CD 4 ( + ) PBLs , and the CD8beta chain reduction was correlated with the MHC 2 decrease within CD8alpha ( + ) PBLs . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
The former class 1 MHC molecules generally present internally synthesized tumor derived peptide antigens to highly specific CD 8 ( + ) cytotoxic T lymphocytes ( CTLs ) in acquired immunity . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
This study investigated whether retinal specific Ags are detected by class 1 MHC restricted CD 8 T cells . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Priming of CD 8 ( + ) T cells requires presentation of short peptides bound to MHC class 1 molecules of professional APCs . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
The exacerbation of EAE in PPARgamma ( + / ) mice associates with an increased expansion of CD 4 ( + ) and CD 8 ( + ) T cells and expression of CD 40 and MHC class 2 molecules in response to MOGp 35 55 Ag . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Simultaneous depletion of CD 25 ( + ) and CD 8 ( + ) cells , as well as adoptive transfer experiments , revealed that tumor specific CD 4 ( + ) T cells , which emerged in the absence of CD 25 ( + ) T reg cells , were able to reject CT 26 colon cancer cells , a MHC class 2 negative tumor . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Using the B 16 melanoma , a poorly immunogenic experimental tumor that expresses low levels of MHC class 1 products , we investigated whether DCs loaded ex vivo with apoptotic tumor cells could elicit combined CD 4 ( + ) and CD 8 ( + ) T cell dependent , long term immunity following injection into mice . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Murine infection with the Gram positive intracellular bacterium Listeria monocytogenes activates CD 8 ( + ) T cells that recognize bacterially derived N formyl methionine peptides in the context of H 2 M3 MHC class Ib molecules . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Animal models of tuberculosis point to a protective role for MHC class 1 restricted CD 8 ( + ) T cells , yet it is unclear how these cells protect or whether such findings extend to humans . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Recently it was shown that activation of class Ib major histocompatibility complex ( MHC ) restricted CD 8 ( + ) T cells alone is sufficient for immune protection against listeriae . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Extensive MHC class 1 restricted CD 8 T lymphocyte responses against various yeast genera in humans . ^^^ While the CD 4 T cell response was marginal , extensive MHC class 1 restricted CD 8 T cell responses were detected against a number of species including spoiling , environmental and human pathogenic yeasts . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
This delivery results in intracellular processing and presentation by MHC class 1 molecules of the CD 8 ( + ) T cell epitopes inserted into CyaA . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Aggregates contained granulocytes , macrophages , and cells expressing major histocompatibility complex ( MHC ) class 2 , CD 4 , and CD 8 , all more numerous in allograft associated conjunctiva . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Anti CD 8 antibodies can inhibit or enhance peptide MHC class 1 ( pMHCI ) multimer binding : this is paralleled by their effects on CTL activation and occurs in the absence of an interaction between pMHCI and CD 8 on the cell surface . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Detailed analysis of two monkeys revealed CD 8 ( + ) T cell responses to several peptide epitopes of Gag not previously described , at least two of which are restricted by MHC class 1 alleles not currently identified . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
As MHC class 1 molecules are essential for the initiation of diabetes in nonobese diabetic ( NOD ) mice , we sought to determine whether the initial insult that allows cross presentation of beta cell autoantigens in diabetes is caused by cognate interactions between naive CD 8 ( + ) T cells and beta cells . ^^^ RIP E 19 expression inhibited MHC class 1 transport exclusively in beta cells and rendered these cells resistant to lysis by CD 8 ( + ) ( but not CD 4 ( + ) ) T cells , both in vitro and in vivo . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Using L ( d ) specific TCR Tg 2C CD 8 ( + ) T cells adoptively transferred into wild type B 6 ( H 2 ( b ) ) mice and the transplantation of BALB / c ( H 2 ( d ) ) tracheal allografts , we now show that the direct recognition of donor specific class 1 MHC molecules by host CD 8 ( + ) T cells leads to their activation , clonal expansion within the graft , and differentiation to an effector phenotype with the capacity to induce airway fibrosis . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Disruption of the class 1 MHC pathway by herpes simplex virus ( HSV ) or the human cytomegalovirus ( HCMV ) results in a decrease of the CD 8 ( + ) cytotoxic T lymphocyte ( CTL ) response and prolongs survival of infected cells in the host . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Nef expression resulted in decreased CD 4 levels , but no change to class 1 MHC , and no impairment in the ability of DC to stimulate recall PPD responses , mixed leukocyte responses , or hepatitis B specific CD 8 responses . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
GL 26 gliomas possessed TRP 2 specific CD 8 ( + ) T cells , providing further evidence that these gliomas express the protein products in the context of MHC class 1 . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Frequency of uterine and blood CD11c+ DC , phenotype ( coexpression of CD8alpha and major histocompatibility complex class 2 [ MHC 2 ] antigens ) , and presence of intracellular cytokines ( interleukins 12 and 10 ) were determined by flow cytometry . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Indeed , when BALB / c mice were immunized with purified recombinant HIV 1 envelope gp 120 or influenza hemagglutinin ( HA ) protein together with poly ( 1 : C ) , epitope specific CD 8 ( + ) class 1 MHC molecule restricted CTL were primed from naive CD 8 ( + ) T cells in vivo . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Thymic selection and peripheral activation of CD 8 T cells by the same class 1 MHC / peptide complex . ^^^ These results establish that CD 8 T cells responsive to their nominal peptide Ag can be generated in FTOC supplemented with soluble MHC class 1 molecules equipped with the same peptide . . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
CD 8 is expressed on cytotoxic T cells ( CTL ) and functions as a coreceptor for recognition of major histocompatibility complex ( MHC ) class 1 peptide complexes by the T cell receptor ( TCR ) . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
MHC class 2 molecules play a role in the selection of autoreactive class 1 restricted CD 8 T cells that are essential contributors to type 1 diabetes development in nonobese diabetic mice . ^^^ In addition , it is now clear that when co expressed with other susceptibility genes , some common MHC class 1 variants aberrantly mediate autoreactive CD 8 T cell responses also essential to T1D development . ^^^ However , it was unknown whether the development of diabetogenic CD 8 T cells could also be dominantly inhibited by particular MHC variants . ^^^ We addressed this issue by crossing NOD mice transgenically expressing the TCR from the diabetogenic CD 8 T cell clone AI 4 with NOD stocks congenic for MHC haplotypes that dominantly inhibit T1D . ^^^ In contrast , heterozygous expression of some MHC haplotypes conferring T1D resistance anergized AI 4 T cells through decreased TCR ( H 2 ( b ) ) or CD 8 expression ( H 2 ( q ) ) . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
In addition to TUNEL staining , most sections were double stained with monoclonal antibodies specific to either CD 8 or MHC class 2 molecules to further explore the relationship between CD8+ feather infiltrating lymphocytes and this increase in apoptotic cells . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
First , we describe that conjugation of CpG DNA to OVA enhances up to 40 fold internalization of OVA by B cells , which in turn generate the CD 8 T cell epitope SIINFEKL complexed to MHC class 1 , albeit less efficiently than DCs . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Adoptive transfer into s . c . tumors demonstrated that naive CD 8 ( + ) T cells were highly susceptible to becoming DN TIL , and local supplementation of tumors with nontumor Ag bearing MHC class 1 expressing fibroblasts decreased both this susceptibility and endogenous DN TIL levels . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Recent studies using MHC class 1 tetramers have shown that CD 8 ( + ) T cell responses against different persistent viruses vary considerably in magnitude and phenotype . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Both human and mouse cytomegaloviruses ( HCMV and MCMV ) avoid peptide presentation through the major histocompatibility complex ( MHC ) class 1 pathway to CD 8 ( + ) T cells . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Overall , this report demonstrates that after vaccination with a MHC class 1 restricted melanoma peptide , resected nonmetastatic melanoma patients can mount a significant antigen specific CD 8 ( + ) T cell immune response with a functionally intact memory component . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
DC release abundant MHC class I / peptide complexes transferred within exosomes to other naive DC for efficient CD 8 ( + ) T cell priming in vitro ; 2 ) . exosomes require nature ' s adjuvants ( mature DC ) to efficiently promote the differentiation of melanoma specific effector T lymphocytes producing IFN gamma ( Tc 1 ) effector lymphocytes in HLA A 2 transgenic mice ( HHD 2 ) . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
However , we showed in part 1 that exosomes are efficient to promote primary MHC class 1 restricted effector CD 8 ( + ) T cell responses only when transferred onto mature DC in vivo . ^^^ In this work , we bring evidence that among the clinically available reagents , Toll like receptor 3 and 9 ligands are elective adjuvants capable of triggering efficient MHC restricted CD 8 ( + ) T cell responses when combined to exosomes . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
The initiation of most cytotoxic immune responses requires MHC class 1 restricted presentation of internalized antigens to CD 8 ( + ) T lymphocytes , a process called cross presentation . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
The CD 8 population in CD 4 deficient mice is heavily contaminated with MHC class 2 restricted T cells . ^^^ In experiments to study the impact of deficiency in CD4+ T cell help on the magnitude of CD8+ cytotoxic T cell response to pathogens , it was noted that in CD 4 gene knockout mice , the CD 8 population made significant responses to several nominally major histocompatibility complex ( MHC ) class 2 restricted epitopes in addition to the expected responses to MHC class 1 restricted epitopes . ^^^ Further experiments suggested that these two observations are linked and that the CD 8 population in CD 4 / mice may contain a majority of T cells that were actually selected by recognition of MHC class 2 molecules in the thymus . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Major histocompatibility complex ( MHC ) class 2 restricted thymocytes were , instead , redirected into the CD 8 lineage . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
RESULTS : Coculture of purified T cells with class 2 MHC positive HUVECs and either TSST 1 or SEB resulted in 5 ( beta ) restricted CD 69 up regulation by CD 4 and CD 8 cells ( 5 ( beta ) 2 activation for TSST 1 ; 5 ( beta ) 3 , 5 ( beta ) 5 . 1 , and 5 ( beta ) 12 activation for SEB ) . ^^^ CONCLUSION : Class 2 MHC positive endothelial cells operate as competent superantigen presenting cells for CD 4 and CD 8 lymphocytes in vitro . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Fewer tumor infiltrating CD 8 ( + ) T cells were detected in MHC class 2 negative cases compared with positive cases ( 2 . 8 % versus 11 . 0 % ; P = . 001 ) , supporting the hypothesis that loss of tumor immunosurveillance has a devastating effect on patient outcome in DLBCL . . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
The effect of IL 12 priming is closely associated with qualitative changes in CD 8 T cells , such as reduced MHC 1 tetramer binding and CD 69 expression , altered distribution of lipid rafts , decreased cytolytic activity , and less susceptibility to apoptosis . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
MHC class 2 independent CD25+ CD4+ CD8alpha beta+ alpha beta T cells attenuate CD4+ T cell induced transfer colitis . ^^^ CD4+ alpha beta T cell populations that develop in mice deficient in MHC class 2 ( through ' knockout ' of either the Aalpha , or the Abeta chain of the 1 A ( b ) molecule ) comprise a major ' single positive ' ( SP ) CD4+ CD 8 subset ( 60 90 % ) and a minor ' double positive ' ( DP ) CD4+ CD8alpha beta+ subset ( 10 40 % ) . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Expression of cell surface markers ( MHC class 1 alpha chain , MHC class 2 associated invariant chain , CD 8 beta chain , CD 18 , and beta 2 microglobulin ) , and genes involved in the innate immune response ( NK lysin like ) increased with age , and these patterns were consistent with an increase in the immune responsiveness of the young chick . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Much of the CD 8 ( + ) T cell response in H 2 ( b ) mice with influenza pneumonia is directed at the nucleoprotein ( 366 374 ) ( NP ( 366 ) ) and acid polymerase ( 224 233 ) ( PA ( 224 ) ) peptides presented by the H2D ( b ) MHC class 1 glycoprotein . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
The murine gamma herpesvirus 68 MK 3 protein inhibits CD 8 ( + ) T cell recognition by ubiquitinating the cytoplasmic tails of classical MHC class 1 heavy chains . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
To date , most studies have focused on the use of MHC class 1 restricted peptides , and have not shown a correlation between Ag specific CD 8 ( + ) T cell expansion and the generation of protective immune responses . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Interestingly , FLT3L induced a pronounced redistribution and accumulation of MHC class 2 ( + ) DC in the lymphoid tissues in old mice , markedly increased the accumulation of CD8alpha ( ) DC in the bone marrow in both young and old nude mice , and elevated both CD8alpha ( ) and CD8alpha ( + ) DC in the spleen in young mice . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
CD 8 CTL recognize antigenic peptides in the context of class 1 MHC . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
In contrast to B cells , which recognize soluble Ag , CD 8 ( + ) and CD 4 ( + ) T cells react to Ag derived peptides bound to MHC 1 or MHC 2 molecules , respectively . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
The proportion of CD 4 , CD 8 , MHC class 2 antigen , and non T / non B cells in peripheral blood lymphocytes was significantly higher in the Oregpig receiving pigs than in the control animals . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Monoclonal antibodies directed against CD 18 , CD11a , CD11b , CD11c , CD 21 , CD 3 , CD 4 , CD 8 and Major Histocompatibility Complex class 1 and 2 ( MHC 1 and MHC 2 ) were also employed . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
An understanding of the mode of presentation of tumour antigens , including those complexed to heat shock proteins by major histocompatibility complex ( MHC ) class 1 and class 2 molecules , and their recognition by CD 8 ( + ) and CD 4 ( + ) T cells , respectively , has further delineated the potential cancer rejection pathways involved . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
DC administration to CD 8 alpha knockout mice displayed unexpectedly enhanced levels of protection to tumor challenge despite this deficiency in CD 8 ( + ) T cells and the inability to mount MHC class 1 restricted immune responses . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Selection of immature CD4CD8 double positive ( DP ) thymocytes for CD 4 or CD 8 lineage commitment is controlled by the interaction of the TCR with stromal cell expressed peptide / MHC . ^^^ We show that thymocyte intrinsic genes influence the pattern of expression of a MHC class 1 restricted transgenic ( tg ) TCR so that in DBA / 2 mice , DP thymocytes with a characteristically high expression of tg TCR , infrequently transit to CD 8 single positive thymocytes . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Further characterization indicated that the CD 8 ( + ) T cell lines obtained were stained with MHC class 1 tetramer ( HLA A2 / MBP ( 111 119 ) ) and exhibited specific cytotoxicity toward autologous target cells pulsed with MBP derived peptides in the context of MHC class 1 molecules . ^^^ These findings support the potential role of CD 8 ( + ) CTLs recognizing MBP in the injury of oligodendrocytes expressing both MHC class 1 molecules and MBP . . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
To address this , we placed MHC class 1 deficient D ( b ) K ( b ) knockout skin grafts onto allogeneic H 2 ( k ) SCID recipients followed by adoptive transfer of purified H 2 ( k ) CD 8 ( + ) T cells . ^^^ The MHC class 1 deficient grafts were rejected and only CD 8 ( + ) T cells were detectable in the recipient lymphoid organs and in the skin grafts . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Further , mice lacking CD 4 ( + ) T cells and mice lacking both CD 8 and MHC 2 showed very little reaction at all to FITC , while the absence of CD 8 T cells alone or MHC 2 alone conferred partial protection only . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
APCs process mammalian heat shock protein ( HSP ) : peptide complexes to present HSP chaperoned peptides on class 1 MHC ( MHC 1 ) molecules to CD 8 ( + ) T cells . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Lymphocytic choriomeningitis virus infection of H 2 ( b ) mice generates a strong CD 8 ( + ) CTL response mainly directed toward three immunodominant epitopes , one of which , gp 33 , is presented by both H 2D ( b ) and H 2K ( b ) MHC class 1 molecules . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
In most cases , the development of such vaccines requires strategies capable of stimulating CD 8 ( + ) cytotoxic T lymphocytes ( CTLs ) and thus , to deliver antigen to MHC class 1 molecules . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Previously , a peptide epitope derived from the Hsp 60 molecule of Salmonella that is presented by the major histocompatibility complex ( MHC ) class Ib molecule Qa 1 to CD 8 ( + ) cytotoxic T cells ( CTLs ) was described . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
The induction of specific CD 8 ( + ) CTL by OVA CT treated DC was associated with enhanced presentation of OVA peptide ( SIINFEKL ) on MHC class 1 in combination with an overall activation of the pulsed DC . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Heat shock proteins ( HSPs ) are a class of host derived inflammatory mediators that perform the dual function of both chaperoning MHC class 1 restricted epitopes into the cross presentation pathway of DCs and inducing the activation / maturation of these DCs to allow priming of cognate CD 8 ( + ) T cell effector responses . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
However , a role for CD 8 ( + ) T cells in primary protection was demonstrated in MHC class 2 ( / ) mice , where depleting CD 8 ( + ) T cells lead to increase severity of disease . ^^^ Unlike control MHC class 2 ( / ) mice , the group depleted of CD 8 ( + ) T cells developed skin lesions on the tail and feet and had adrenal necrosis . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
MAPPP is a bioinformatics tool for the prediction of potential antigenic epitopes presented on the cell surface by major histocompatibility complex class 1 ( MHC 1 ) molecules to CD 8 positive T lymphocytes . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
These fusion cells expressed major histocompatibility complexes ( MHC ) class 1 and 2 , CD 86 , CD11c and CD8alpha . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Using MHC class 1 and class 2 multimers , we tracked the responses of Ag specific CD 8 ( + ) and CD 4 ( + ) memory T cells in the same animals . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Furthermore , a potent IFN gamma inducing , D ( b ) restricted CD 8 ( + ) epitope was identified using MHC class 1 mutant B6 . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Location of the epitope for an anti CD8alpha antibody 53 . 6 . 7 which enhances CD8alpha MHC class 1 interaction indicates antibody stabilization of a higher affinity CD 8 conformation . ^^^ MHC class 1 tetramers are widely used , usually in combination with an antibody to CD 8 , to detect antigen specific T cells . ^^^ Some anti CD8alpha antibodies block the interaction of murine MHC class 1 tetramers with CD 8 T cells , while others such as 53 . 6 . 7 , enhance . ^^^ In the cocrystal of CD8alphaalpha with MHC class 1 , two different complexes ( A and B ) were observed , indicating the existence of different CD 8 conformations . ^^^ The most likely explanation for antibody enhancement of tetramer bindings is that binding of 53 . 6 . 7 to CD8alphabeta stabilizes a conformation with a higher affinity for interaction with MHC class 1 and suggests that the CD 8 binding site is flexible . . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
RPE cells transduced with Axokine stimulated MHC 1 expression , and the cell transplantation changed the ratio of CD 4 ( + ) and CD 8 ( + ) T cells . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Progress has been made primarily using MHC class 1 tetramers to monitor CD 8 ( + ) T cells , whereas corresponding efforts to stain CD 4 ( + ) T cells with class 2 tetramers have not been as successful . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Self reactive memory phenotype CD 8 T cells exhibit both MHC restricted and non MHC restricted cytotoxicity : a role for the T cell receptor and natural killer cell receptors . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
CD 8 ( + ) T cells were found to infiltrate the epidermis which was associated with an aberrant expression of Ki 67 , cytokeratin 16 , MHC class 2 , and ICAM 1 on adjacent keratinocytes . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
As a result of this intracellular lifestyle , the pathways for the induction of MHC 1 and CD 1 restricted CD 8 T cells by such microorganisms remain elusive . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Similarly , in a model of CD 8 dependent GVHD across MHC class 1 mismatch injection of allogeneic DCs , but not B cells , induced robust proliferation of donor CD 8 ( + ) T cells and broke GVHD resistance of chimeric recipients in which APCs were syngeneic to donors . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Development of antigen specific CD8+ CTL in MHC class 1 deficient mice through CD 4 to CD 8 conversion . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
These results show that the MHC region is involved in the genetic regulation of CD 8 ( + ) T cell numbers in humans . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
The purpose of this study was to determine the role for CD 8 T cells versus generalized MHC class 1 restricted antigen presentation in islet allograft rejection and tolerance . ^^^ Diabetic C57BI / 6 ( B 6 , H 2 ( b ) ) controls , C57BI / 6 CD 8 deficient ( CD 8 KO ) , or MHC class 1 deficient C57BI / 6 ( beta 2m KO ) recipients were grafted with allogeneic BALB / c ( H 2 ( d ) ) islets . ^^^ Islet allografts were acutely rejected in untreated B 6 , CD 8 KO , and in beta 2m KO mice , indicating that neither CD 8 T cells nor host MHC class 1 is required for allograft rejection . ^^^ Although anti CD 154 induced allograft survival was CD 8 T cell independent , an intact host MHC class 1 restricted ( beta 2m dependent ) pathway is nevertheless necessary for allograft survival . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
MHC class 1 antigen and LFA 1 expression on CD 8 ( + ) T cells and MHC class 2 antigen on B cells were also decreased . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Polymorphism within the MHC not only affects peptide specificity but also has a critical influence on the T cell repertoire ; for example , the CD 8 T cell response toward an immunodominant HSV glycoprotein B peptide is more diverse and of higher avidity in H 2 ( bm 8 ) compared with H 2 ( b ) mice . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
The bacterium L . monocytogenes is a proposed vaccine carrier based upon the observation that this pathogen replicates within the intracytoplasmic environment facilitating delivery of Ag to the endogenous Ag processing and presentation pathway with subsequent stimulation of peptide specific MHC class 1 restricted CD 8 ( + ) effector cells . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
In vitro , OVA archaeosomes stimulated spleen cells from OVA TCR transgenic mice , D011 . 10 ( CD 4 ( + ) cells expressing OVA ( 323 339 ) TCR ) or OT 1 ( > 90 % CD 8 ( + ) OVA ( 257 264 ) cells ) , indicating both MHC class 1 and 2 presentations . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
CD8alpha and MHC class 2 expression on cDC and pDC was higher on DC from FL treated mice than on DC from control littermates . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Major histocompatibility complex ( MHC ) tetramers ( multimeric complexes capable of binding to specific CD 8 T cell clones ) were conjugated to ( 225 ) Ac ( an alpha emitting atomic nanogenerator , capable of single hit killing from the cell surface ) to create an agent for CD 8 T cell clonal deletion . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Differences were found between the three feeding treatments in the relative expression of MHC class 2 molecules , the relative expression of BU 1 molecules , and the ratio of CD 4 : CD 8 single positive cells . 6 . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
METHODS : Whereas as to date , no direct ex vivo analysis can be performed of the CD 8 T cell repertoire directed at allo major histocompatibility complex ( MHC ) class 1 molecules , virus specific cells can be readily enumerated by use of MHC peptide tetrameric complexes . ^^^ In this study , the authors used this technique to analyze potential overlapping CD 8 T cell repertoires between self MHC viral peptide and allo MHC complexes by stimulating CMV specific CD 8 T cells with alloantigens . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
The human major histocompatibility complex ( MHC ) class Ib gene , HLA E , codes for the major ligand of the inhibitory receptor NK G 2A , which is present on most natural killer ( NK ) cells and some CD 8 ( + ) cytotoxic T lymphocytes . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
We , therefore , determined the role of IL 18 in GVHD mediated by CD 4 ( + ) or CD 8 ( + ) T cells across major histocompatibility complex ( MHC ) class 2 and class 1 disparate allogeneic BMT , respectively . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Although their function remains enigmatic , exosomes are a source of antigen and transfer functional major histocompatibility complex ( MHC ) I / peptide complexes to dendritic cells ( DCs ) for CD 8 ( + ) T cell activation . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Functional characterization of MHC class 2 restricted CD8+CD4 and CD 8 CD4 T cell responses to infection in CD 4 / mice . ^^^ Classical CD 4 ( + ) and CD 8 ( + ) T cells recognize Ag presented by MHC class 2 ( MHCII ) and MHC class 1 ( MHCI ) , respectively . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Encapsulation of MHC 1 epitopes in pH triggered microparticles increases Ag presentation and may improve CD 8 ( + ) T cell priming to peptide vaccines against viruses and cancer . . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
We further established a flow cytometric procedure for lymphocyte subsets and clarified the mean percentages of T and B cells , CD 4 ( + ) , CD 8 ( + ) and MHC Class 2 ( + ) T cells , and CD 4 ( ) CD 8 ( ) T cells . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Anti tumor MHC class Ia unrestricted CD 8 T cell cytotoxicity elicited by the heat shock protein gp 96 . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
These results suggest that depletion of CD 8 ( + ) cells from DLI could impair GvL against CML , while increased MHC disparity between donor and recipient may improve the responsiveness of Philadelphia ( + ) B lymphoblastic leukemia to DLI . . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
In addition , the expression of maturation markers such as MHC 2 , CD 40 , CD 80 and CD 86 , and of differentiation markers such as CD 4 , CD 8 and CD11b , was also comparable in the two populations . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
ED 1 ( monocytes and immature macrophages ) , ED 2 ( mature tissue macrophages ) , CD 4 ( helper / inducer T lymphocytes and macrophages ) , CD 8 ( suppressor / cytotoxic T lymphocytes ) , Interleukin 1beta , IL 2 ( activated T lymphocytes ) , Major Histocompatibility Complex ( MHC ) class 2 ( activated macrophages and activated B lymphocytes ) , alpha beta ( T lymphocytes ) and CD45RA ( B lymphocytes ) antibodies have been used to stain immunohistochemically each sample . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
We introduced previously an on line resource , RANKPEP that uses position specific scoring matrices ( PSSMs ) or profiles for the prediction of peptide MHC class 1 ( MHCI ) binding as a basis for CD 8 T cell epitope identification . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
For unknown reasons , the common MHC class 1 variants encoded by the H2g7 haplotype ( Kd , Db ) aberrantly elicit autoreactive CD 8 T cell responses essential to type 1 diabetes development when expressed in NOD mice , but not other strains . ^^^ In this study , we show that interactive non MHC genes allow a NOD derived diabetogenic CD 8 T cell clonotype ( AI 4 ) to be negatively selected at far greater efficiency in C57BL / 6 mice congenically expressing H2g7 ( B6 . ^^^ H2g7 stock , non MHC gene controlled elevations in TCR expression are associated with both enhanced negative selection of diabetogenic CD 8 T cells and increased aggressiveness of those escaping this process . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
We found that OVA specific CD 8 ( + ) T cells were partially tolerant to intestinal epithelium derived OVA , because oral infection with Listeria monocytogenes encoding OVA did not elicit an endogenous OVA specific MHC class 1 tetramer ( + ) CD 8 ( + ) T cell response and IFN gamma , IL 4 , and IL 5 secreting T cells were decreased in the Peyer ' s patches , mesenteric lymph nodes , and intestinal mucosa of transgenic mice . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
These alphabeta T cells recognize antigenic peptides presented by MHC class 1 ( for CD 8 ( + ) T cells ) or MHC class 2 molecules ( for CD 4 ( + ) T cells ) . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Donor CD 8 ( + ) T cells can be potent mediators of graft versus host disease ( GVHD ) after allogeneic hematopoietic cell transplantation to either major histocompatibility complex ( MHC ) class 1 or multiple minor histocompatibility antigen mismatched recipients . ^^^ To develop small molecular inhibitors of CD 8 ( + ) T cell activity , theoretical structural analysis of the human CD 8 alpha molecule was previously used to identify potential functional surface epitopes that interact with the MHC class 1 molecule . ^^^ Accordingly , studies were conducted to determine whether there was sufficient cross species homology in the DE loop region and its nonpolymorphic interactive site on the beta ( 2 ) microglobulin domain of the MHC class 1 molecule to allow similar inhibition of murine CD 8 ( + ) cytotoxic T lymphocyte activity . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Eight days later , at the peak of expansion phase of T cell response , we used tetramers of MHC class 1 molecules containing viral peptides to directly visualize antigen specific CD 8 T cells and a sensitive functional assay measuring interferon gamma production at the single cell level to quantitate the CD 8 and CD 4 T cell response . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
In the present study , clonal rainbow trout ( Oncorhynchus mykiss ) embryos and larvae were assayed for the expression of key molecules involved in specific cell mediated cytotoxicity using an anti MHC class 1 monoclonal Ab and by RT PCR using specific primers derived from classical MHC class 1 ( class Ia ) , TCR and CD 8 . ^^^ Whereas RT PCR revealed that MHC class Ia and CD 8 were expressed from at least 1 week after fertilisation ( p . f . ) on , TCR expression was detectable from 2 weeks p . f . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
CONCLUSIONS : The single platform MHC tetramer based method for antigen specific CD 8 ( + ) T cell counting has been evaluated by a European group of laboratories and can be considered a reproducible assay for routine enumeration of antigen specific CD 8 ( + ) T cells . . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Eight indicator traits of leukocyte responsiveness to dexamethasone included the percentages of circulating B cells , T cells ( CD 4 , CD 8 , and workshop cluster 1 molecule expressed by bovine gammadelta T cell ) , major histocompatibility complex ( MHC ) 1 and 2 expressing cells , and mean expressions of surface MHC 1 and MHC 2 on circulating cells . ^^^ Genetic variation in linear recovery from nadir values following dexamethasone administration was significant only for percentage CD 4 , percentage CD 8 , and for surface expression of MHC 2 . ^^^ The genetic covariance between basal and linear recovery was positive and significant for percentage CD 4 , percentage CD 8 , and MHC 2 expression . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Numerous MHC class 2 ( + ) , CD 4 ( + ) , and CD 8 ( + ) T cells were identified with acute rejection . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Redirecting human CD4+ T lymphocytes to the MHC class 1 restricted melanoma antigen MAGE A 1 by TCR alphabeta gene transfer requires CD8alpha . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Flow cytometry was used to quantify MHC 2 , B 220 , CD 4 , and CD 8 cells . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
METHODS : Immunohistochemistry was performed on frozen sections of 60 primary breast cancers by use of monoclonal antibodies to T lymphocytes ( CD 3 ) , T helper cells ( CD 4 ) , cytotoxic T cells ( CD 8 ) , natural killer cells ( CD 56 ) , interleukin 2 receptors ( IL 2R ) , and major histocompatibility ( MHC ) class 1 antigen ( HLA ABC ) and MHC class 2 antigen ( HLA DR ) . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
This allows use of engineered CyaA for targeted delivery of CD 8 ( + ) T cell epitopes into the MHC class 1 pathway of APC and induction of robust and protective cytotoxic responses . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
When peptides containing ovalbumin ( OVA ) derived CD 4 and CD 8 T cell epitopes were conjugated to 0 . 05 microm nano beads , they gave strong immune responses and inhibition of growth of tumour cells expressing the CD 8 T cell epitope with MHC class 1 . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Antigen recognition by cytotoxic CD 8 T cells is dependent upon a number of critical steps in MHC class 1 antigen processing including proteosomal cleavage , TAP transport into the endoplasmic reticulum , and MHC class 1 binding . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
These results indicate that nef does serve to limit the virus specific CD 8 cellular response of the host and that the ability to downregulate MHC class 1 contributes importantly to the totality of nef function . . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Here , we show that monocyte derived DC generated in the presence of IL 10 exhibit a profound down regulation of many genes that are associated with immune activation and show that the IL 10 grown DC are poor stimulators of CD 8 ( + ) T cells in a strictly autologous and major histocompatibility complex ( MHC ) class 1 restricted melanoma antigen recognized by T cells ( MART 1 ) epitope presentation system . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
This investigation was initiated to determine the role of CTLA 4 in the activation of CD 4 ( + ) CD 8 ( + ) double positive ( DP ) and CD 4 ( + ) CD 8 ( ) and CD 4 ( ) CD 8 ( + ) single positive ( SP ) thymocytes using fetal thymic organ cultures ( FTOC ) of MHC class 2 restricted , OVA ( 323 339 ) restricted TCR transgenic mice ( DO11 . 10 ) . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
The fate of naive CD 8 ( + ) T cells is determined by the environment in which they encounter MHC class 1 presented peptide Ags . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Nevertheless , CD 8 ( + ) T cells recognizing Chlamydia Ags in the context of MHC class 1 molecules are primed during infection . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Enforced endocytosis of Ag together with the adjuvant effect of Toll like receptor 9 ligands might be key for the efficient cross presentation of exogeneous Ag as well as for effective cross priming of MHC class 1 restricted CD 8 T effector cells . . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
We were able to demonstrate that AAV / BA46 / Neo loading of DCs resulted in : ( 1 ) BA 46 expression in DCs , ( 2 ) chromosomal integration of the AAV / BA46 / Neo vector within DCs , ( 3 ) strong , rapid BA 46 specific , MHC class 1 restricted CTLs in only 1 week , ( 4 ) T cell populations with significant interferon gamma ( IFN gamma ) expression but low IL 4 expression , ( 5 ) high CD 80 and CD 86 expression in DCs , and ( 6 ) high CD 8 : CD 4 and CD 8 : CD 56 T cell ratios . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
The J TCBL / ICBL is a peptide from beta 2 microglobulin which binds to the CD 8 protein and promotes Th 1 responses and the G TCBL / ICBL is a peptide from the beta chain of MHC 2 molecules that binds to the CD 4 protein and promotes Th 2 responses . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
In the study reported here , cell types in colonic tissues from these pigs were analyzed by use of immunohistochemical and morphometric analyses for cell surface markers ( IgM , IgG , IgA , CD 4 , CD 8 , MHC Class 2 , and macrophage SWC3a ) . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Codelivery of IL 12 , IL 15 , or anti CD 40 together with MHC class 1 restricted peptide combined with TLR 9 ligand at inception of immunization resulted in generation of memory CD 8 ( + ) T cells that were several fold less compromised than immunization with peptide alone . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
MHC class 1 specific inhibitory receptors are expressed by a subset of memory phenotype CD 8 ( + ) T cells . ^^^ We analyzed here human CD 8 ( + ) T cells that express the Ig like MHC class 1 specific inhibitory receptors : killer cell Ig like receptor ( KIR ) and CD85j . ^^^ The cell surface expression of Ig like inhibitory MHC class 1 receptors was found to correlate with an advanced stage of CD 8 ( + ) T cell maturation as evidenced by the reduced proliferative potential of KIR ( + ) and CD85j ( + ) T cells associated with their high intracytoplasmic perforin content . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
CD 8 ( + ) CD 28 ( ) T ( S ) cells are FOXP3+ , MHC class 1 restricted and tolerize both professional antigen presenting cells , such as dendritic cells ( DC ) and nonprofessional APC such as endothelial cells ( EC ) by up regulating the cell surface expression of inhibitory receptors immunoglobulin like transcript ( ILT ) 3 and ILT 4 and down regulating the expression of costimulatory molecules such as CD 58 and CD 86 . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Dendritic cells ( DCs ) have the unique ability to capture cellular tissue antigens , and to present them on MHC class 1 molecules to antigen specific CD 8 ( + ) T lymphocytes after migration to the draining lymph nodes . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
The total number of circulating leukocytes was unchanged and increase in message was accompanied by an increase in circulating CD8alpha ( + ) and MHC Class 2 ( + ) cells . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
To further characterize the magnitude and kinetics of expansion of the MOG specific CD 8 ( + ) T cell population in vivo , we used MOG 37 50 / H 2D ( b ) MHC tetramers to visualize MOG specific CD 8 ( + ) effectors in the peripheral lymphoid organs and central nervous system during the course of EAE induction and progression . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Immunohistochemical detection of CD 4 , CD 8 and MHC 2 expressing immune cells and endoglin in the canine corpus luteum at different stages of dioestrus . ^^^ Specific immunohistochemical methods were applied to detect the presence of CD 4 , CD 8 and major histocompatibility complex 2 ( MHC 2 ) expressing immune cells and of endoglin in the canine corpus luteum between days 15 and 75 , after ovulation . ^^^ Quantitative evaluation yielded high values on day 15 , followed by a decrease on day 30 ( CD 4 , CD 8 and endoglin ) and day 45 ( MHC 2 ) . ^^^ While there were no further changes for cells staining positive for CD 4 and endoglin , CD 8 positive immune cells increased from day 45 to day 60 to drop again on day 75 ; MHC 2 positive staining increased from day 45 to days 60 75 . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Also in Xenopus , gp 96 derived from MHC class 1 negative tumors elicits peptide specific responses against these tumors in vivo and MHC unrestricted CD 8 killing in vitro . ^^^ Similar accumulation and division occurred when CD 8 T cells primed by 15 / 0 tumor derived gp 96 were transferred to an isogeneic recipient bearing the same MHC class 1 negative tumor . ^^^ In addition , at least in Xenopus , gp 96 can prime CD 8 ( + ) T cell effectors that are not MHC restricted . . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
DC 888 mel hybrids presented all tumour associated epitopes to both CD 4 and CD 8 T cell lines in the context of MHC class 2 and 1 molecules , respectively . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
We used major histocompatibility complex ( MHC ) class 1 tetramers to stimulate T cells to focus on the interaction of T cell receptor ( TCR ) / MHC and CD 8 in the absence of other molecules that are present on cell surfaces and so contribute to the activation of T cells by undefined mechanisms . ^^^ Mutated MHC class 1 tetramers that are unable to engage CD 8 were used to investigate the role of CD 8 engagement in memory cell activation . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
B4 ( CD 244 ) is a non MHC binding receptor with multiple functions on natural killer cells and CD8+ T cells . 2B4 ( CD 244 ) is expressed by memory phenotype CD 8 ( + ) T cells and all natural killer ( NK ) cells . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Cross presentation is the pathway by which exogenous antigens are routed for presentation on MHC class 1 for activation of CD 8 ( + ) T cells . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
We determined the role of non CTL effector mechanisms through the transfer of either wildtype or interferon gamma ( IFN gamma ) deficient CD 8 ( + ) T cells into RAG 1 ( / ) recipients of MHC class 1 deficient allografts . ^^^ Transfer of wildtype CD 8 ( + ) T cells into recipients of MHC class 1 deficient grafts resulted in a reduction in AV lesion size , while transfer of interferon gamma deficient CD 8 ( + ) T cells into MHC class 1 deficient grafts abrogated AV . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
In three different systems , analyses of mice expressing transgenic T cell receptors specific for antigen plus MHC class 1 or class 2 molecules have demonstrated several important features of T cell development : ( 1 ) T cell receptor specificity for MHC class 1 or class 2 molecules determines the expression of CD 8 versus CD 4 , respectively , on mature T cells ; ( 2 ) T cell maturation in the thymus is dependent on expression of an MHC molecule recognized by the T cell receptor ; ( 3 ) for class 2 specific receptors , appropriate MHC expression on thymic epithelial cells is sufficient to achieve positive selection of T cells ; and ( 4 ) self reactive T cells die or are killed in the thymus . . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Expression of classical MHC class 1 and 2 molecules , ICAM 1 and the epithelial CD 8 ligand , gp 180 was not significantly affected by iron . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Recently , we have shown that two immunodominant MHC class 1 restricted epitopes , NP ( 366 374 ) / D ( b ) ( nucleoprotein ( NP ) ) and PA ( 224 233 ) / D ( b ) ( acidic polymerase ( PA ) ) , which drive the CD 8 ( + ) T cell response to influenza virus infection in C57BL / 6 mice , are differentially expressed on infected cells . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
At 18 26 months EBNA 1 transgenic mice did not differ from littermate control in ultimate body weight , in spleen size or weight , in lymph node , kidney , liver , or spleen histology , in splenocyte fractions positive for cluster of differentiation ( CD ) 3epsilon , CD 4 , CD 8 , CD62L , B 220 , CD 5 , IgM , IgD , MHC class 2 , CD11b , or CD 25 , or in serum IgM , IgG , or total Ig levels . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
It also enhances cell mediated immunity by targeting antigen delivery in antigen presenting cells to both the MHC class 1 pathway of exogenous presentation that activates CD 8 T cells and the MHC class 2 pathway that processes antigen endogenously and presents it to CD 4 T cells . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Proliferation of gamma / delta T cells was MHC class 2 and CD 8 dependent . . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
In accordance with this , effects of G alpha i 2 deficiency on CD 8 or CD 4 SP thymocyte differentiation required engagement of the TCRs with either MHC class 1 or MHC class 2 molecule . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Within the epithelial layer of dysplasias , there were increased number of lymphocytes , which were immunopositive for CD45RO , CD 8 , and CD 57 and CD 68 immunopositive ( + ) , S 100 protein positive and major histocompatibility complex ( MHC ) class 2 positive monocytic lineages . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
It has been previously shown that this bacterial protein could be used to deliver CD 4 ( + ) and CD 8 ( + ) T cell epitopes to the MHC class 2 and class 1 presentation pathways to trigger specific Th and CTL responses in vivo , providing protection against subsequent viral or tumoral challenge . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
The presentation of viral peptide MHC class 1 complexes by antigen presenting cells , such as dendritic cells ( DCs ) , is obligatory for the generation of antiviral effector and memory CD 8 ( + ) cytotoxic T lymphocyte ( CTL ) responses . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Together these studies shed light on the tapasin protein life cycle and how it functions in MHC class 1 assembly with peptide for presentation to CD 8 ( + ) T cells . . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
CMV , influenza virus , EBV , and HIV specific CD 8 ( + ) T cells as measured by the IFN gamma ELISPOT and MHC class 1 tetramer staining were all present at greater frequencies in breast milk as compared with blood . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Furthermore , T cell clones isolated from these cultures showed both CD 8 ( + ) and CD 4 ( + ) transduced T cells could recognize HLA A 2 ( + ) melanoma cells , giving us the possibility of engineering class 1 MHC restricted effector and T helper cells against melanoma . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Specifically , the effects of 17beta estradiol on basal and LPS induced surface staining of Class 1 and 2 MHC , as well as CD 40 , CD 80 , CD 86 , CD 152 , CD 28 , CD 8 , CD11b , Fas , FasL , and also ERalpha and ERbeta , were examined in N 9 microglial cells . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Major histocompatibility complex ( MHC ) class 1 molecules , which are ligands of antigen specific receptors on CD 8 T cells and of inhibitory receptors on natural killer cells , have an important and surprising role in the control of activity dependent neuronal plasticity in the central nervous system ( CNS ) . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
CD 8 MHC class 1 restricted responses to HIV antigens were assayed . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Sharing of class 1 MHC molecules between donor and host promotes the infiltration of allografts by mHAg reactive CD 8 T cells . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Here we demonstrate that IEL in the immediate postnatal period of MHC homozygous male mice were mostly CD 8 ( ) T cells , while IEL in MHC F ( 1 ) male mice were CD 8 ( ) and CD8alphabeta+ T cells . ^^^ Regardless of the MHC 1 configuration and the ability to support positive selection of CD8alphabeta+ cells in the thymus , the expansion of CD8alphaalpha+ IEL was a late postnatal event that followed a reduction in CD 8 ( ) IEL . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Using human monocyte derived DC and ILT 4 transgenic mice , we show that ( 1 ) HLA G induces the development of tolerogenic DC with arrest maturation / activation of myeloid DC , ( 2 ) HLA G modified DC induce differentiation of anergic and immunosuppressive CD 4 ( + ) and CD 8 ( + ) effector T cells , and ( 3 ) the gene expression profile provides evidence that HLA G induces tolerogenic DC by disruption of the MHC class 2 presentation pathway . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
CD 8 ( + ) T cells recognize peptides of eight to nine amino acid residues long in the context of MHC class 1 molecules on the surface of antigen presenting cells ( APCs ) . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Appropriate activation of naive CD 8 ( + ) T cells depends on the coordinated interaction of these cells with professional APC that present antigenic peptides in the context of MHC class 1 molecules . ^^^ Nevertheless , it is unclear whether epitopes , derived from endogenously synthesized proteins and presented by MHC class 1 molecules on the surface of other APC including B cells and macrophages , can activate naive CD 8 ( + ) T cells in vivo . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
However , semimature DC loaded simultaneously with MHC class 2 and MHC class 1 restricted peptides , could efficiently initiate CD 8 T cell responses leading to autoimmune diabetes in a TCR transgenic adoptive transfer model . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Characterizing the impact of CD 8 antibodies on class 1 MHC multimer binding . ^^^ Many studies have suggested that CD 8 Abs affect the binding of class 1 MHC tetramers / multimers to CD 8 ( + ) T cells , which has led to the interpretation that CD 8 participates directly in multimer binding . ^^^ These data reinforce the paradigm that CD 8 plays an active and direct role in binding of class 1 MHC multimers . . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
However , the isolation of beta cell autoreactive CD 8 ( + ) T cell clones from the islets of NOD mice helped to generate interest in the pathogenic role of this subset , as has accumulating evidence that certain class 1 MHC alleles are additional risk factors for T1D development in humans . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
The study of PBMCs from Cpn infected individuals using fluorescent MHC tetramers revealed that some patients have CD 8 ( + ) T cells capable of recognizing the Cpn HSP 70 HLA A * 0201 epitope . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
In this paper , we present an analysis of viral clearance and liver toxicity in relation to the induction of the virus specific CD 8 T cell response revealed by an MHC class 1 tetramer . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Although endocytosed proteins are commonly presented via the class 2 MHC pathway to stimulate CD 4 ( + ) T cells , professional APCs can also cross present Ags , whereby these exogenous peptides can be complexed with class 1 MHC for cross priming of CD 8 ( + ) T cells . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
We have used intracellular cytokine staining and MHC class 1 tetramer binding in conjunction with granzyme B protease expression and in vivo BrdU uptake to characterize the primary murine CD 8 ( + ) T cell response to pulmonary influenza virus infection . ^^^ Using induction of granzyme B as a surrogate to identify specific activated CD 8 ( + ) T cells , we found that an unexpectedly large fraction ( approximately 70 % ) of lung infiltrating CD 8 ( + ) T cells expressed granzyme B on day 6 of infection when estimates by MHC tetramer / intracellular cytokine staining yielded substantially lower frequencies ( approximately 30 % ) . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Recently we reported that Cpn infected mice generate an MHC class 1 restricted CD 8 ( + ) Tc 1 response against various Cpn Ags , and that CD 8 ( + ) CTL to multiple epitopes inhibit Cpn growth in vitro . ^^^ Immunization and challenge studies with beta ( 2 ) microglobulin ( / ) mice indicated that the reduction of lung Cpn burdens was mediated by the MHC class 1 dependent CD 8 ( + ) T cells to minigene included Cpn CTL epitopes , rather than by pan DR epitope specific CD 4 ( + ) T cells . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
RESULTS : In nave recipient mice , studies in major histocompatibility complex ( MHC ) 1 deficient mice and with depleting anti CD 8 monoclonal antibody ( mAb ) demonstrate that CD 8 T cells are important for Sa 1 rejection . ^^^ Rejection of xenogeneic tumors was also T cell dependent as demonstrated by anti CD 3 mAb , dependent on both CD 4 and CD 8 T cells as demonstrated using MHC 1 and MHC 2 deficient mice , but was more vigorous as demonstrated by the lack of effectiveness of immunosuppressive drugs in this model . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Assay sensitivity can be hindered by non specific binding activity , which is due to the inherent interactions of CD 8 and MHC . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Substantial proliferation of virus specific CD 8 T cells ( including HIV 1 specific CD 8 T cells ) was also observed in CD 4 T cell depleted populations or after stimulation with MHC class 1 tetramer peptide complexes . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Sporozoites were processed for MHC class 1 presentation by dendritic cells ( DC ) to prime pb 9 specific IFN gamma producing CD 8 T cells . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
In this study , MHC class 2 deficient mice infected with Histoplasma capsulatum ( Histoplasma ) kept the fungus in check for up to 16 wk , indicating that CD 8 T cells are able to limit fungal replication . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
The results of our recent studies suggest that CD 8 ( + ) cytotoxic T cells are capable of recognizing small TACA in a conventional class 1 MHC restricted fashion . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
The main immunohistochemical findings were ( 1 ) a predominance of IgG ( + ) plasma cells over IgA ( + ) and IgM ( + ) plasma cells , ( 2 ) significant numbers of macrophages and dendritic cells expressing MHC class 2 molecules , ( 3 ) macrophages and neutrophils expressing L 1 antigen and ( 4 ) a mixture of CD 4 ( + ) and CD 8 ( + ) T cells . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Also , we performed immunohistochemical assessments of CD 3 , CD 4 , CD 8 , CD 20 , CD 38 , CD 138 , CD 68 , CD 163 , CD 83 , CD1a , CD 146 , alphaVbeta 3 , E selectin , intercellular adhesion molecule 1 , vascular cell adhesion molecule 1 , S100A12 , intracellular citrullinated proteins and major histocompatibility complex ( MHC ) human cartilage ( HC ) gp 39 peptide complexes . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
The interactions between the TCR and peptides bound to class 1 MHC encoded molecules ( pMHC ) and a mechanism for CD 8 cooperation in this process are reviewed . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Immunization of C57Bl / 6 mice with this rLM strain efficiently primed CD 8 T cells to recognize the MHC class 1 restricted TRP 2180 188 epitope and express IFN gamma upon in vitro peptide stimulation . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
SIV Gag specific T cell responses were detected in peripheral blood by MHC class 1 tetramer staining ( peak , 0 . 07 to 0 . 2 % CD 8 ( + ) T cells at week 2 ) and gamma interferon ( IFN gamma ) enzyme linked immunospot ( ELISPOT ) assays ( peak , 50 to 250 spot forming cells / 10 ( 6 ) peripheral blood mononuclear cell at week 3 ) . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Evidence will be presented that exosomes can be directly immunogenic for CD 8 ( + ) T cells in the absence of normal APC with the proviso that exosomes express a high density of MHC I / peptide complexes as well as B 7 and ICAM 1 . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Expression patterns of the fish gene homologues for TCR , CD 8 and MHC class 1 , as well as related genes , are in agreement with similar function . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
These CD 4 ( + ) Th APCs stimulate naive OT 1 CD 8 ( + ) T cell proliferation through signal 1 ( MHC I / OVAI / TCR ) and signal 2 ( e . g . , CD54 / LFA 1 and CD80 / CD28 ) interactions and IL 2 help . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
In contrast , such Ag specific memory CD 8 ( + ) T cells were not detectable by peptide major histocompatibility complex ( MHC ) multimer staining in animals that had not previously received an antigenic challenge . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
The MHC Class 2 mismatch allograft group showed normal epithelium and mild sub epithelial infiltration by CD4+ cells at 4 weeks ( CD 8 , CD11b ) . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Despite breakthroughs in the cloning of the fish TcR and major histocompatibility ( MHC ) molecules , and more recently the CD 3 and CD 8 marker molecules , assays to measure specific T cell responses are still in their infancy . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Traditionally , this function has been ascribed to CD 8 T lymphocytes with cytotoxic activity , which are restricted by MHC class 1 molecules . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
CD8alpha ( + ) dendritic cells ( DCs ) have been shown to be the principal DC subset involved in priming MHC class 1 restricted CTL immunity to a variety of cytolytic viruses , including HSV type 1 , influenza , and vaccinia virus . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
MHC class 1 positive dendritic cells ( DC ) control CD 8 T cell homeostasis in vivo : T cell lymphopenia as a prerequisite for DC mediated homeostatic proliferation of naive CD 8 T cells . ^^^ Using transgenic mice in which only DC express MHC class 1 , we demonstrate that this type of APC is sufficient to trigger complete homeostatic proliferation of CD 8 T cells in vivo . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
We characterized CD 8 ( + ) T cells constitutively expressing CD 25 in mice lacking the expression of MHC class 2 molecules . ^^^ Peripheral CD 8 ( + ) CD 25 ( + ) T cells from MHC class 2 deficient mice also share phenotypic and functional features with regulatory CD 4 ( + ) CD 25 ( + ) T cells : in particular , they strongly express glucocorticoid induced TNFR family related gene , CTLA 4 and Foxp 3 , produce IL 10 , and inhibit CD 25 ( ) T cell responses to anti CD 3 stimulation through cell contacts with similar efficiency to CD 4 ( + ) CD 25 ( + ) T cells . ^^^ However , unlike CD 4 ( + ) CD 25 ( + ) T cells CD 8 ( + ) CD 25 ( + ) T cells from MHC class 2 deficient mice strongly proliferate and produce IFN gamma in vitro in response to stimulation in the absence of exogenous IL 2 . . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
In multiple sclerosis , genetic associations with MHC class 1 alleles have now been established , and CD 8 ( + ) as well as CD 4 ( + ) T cells have been found to invade and clonally expand in inflammatory central nervous system plaques . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
We used a thymus reaggregate culture system in which CD 4 ( + ) CD 8 ( + ) thymocytes from AND TCR transgenic mice were induced to undergo positive selection by pigeon cytochrome c ( PCC ) peptide or its analogs presented by 1 E ( k ) class 2 MHC on a thymic epithelial cell line . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
We obtained > 90 % CD11c ( + ) DC using magnetic cell sorting , of which the majority were CD11b ( + ) CD8alpha ( ) , with smaller numbers of CD11b ( ) CD8alpha ( + ) and CD11b ( ) CD8alpha ( ) DC as well as a distinct population of CD11c ( int ) class 2 MHC ( lo ) B 220 ( + ) DC . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Immunofluorescence microscopy demonstrated that abundant CD4+ T cells and MHC class 2 positive macrophages , but not CD 8 ( + ) T cells , had infiltrated tumour tissue in mice treated with DC / BNL + IL 12 . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Thus , the increase in number of CD 8 ( + ) T cells and enhanced MHC Iota and IotaIota expression in B16F10 cells that result from augmented IFN gamma production in response to anti 4 1BB mAb may lead to suppression of tumour growth and metastasis . . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
This reversal is not caused by redirection of specific MHC class 2 restricted cells to the CD 8 lineage . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
That is , MHC 2 signaled DP thymocytes differentiated into CD 8 lineage cytotoxic T cells , despite the MHC 2 specificity and CD 4 dependence of their TCRs . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
This vaccine also induced MHC class 1 antigen restricted CD 8 ( + ) T cell responses . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Cutting edge : a single MHC class Ia is sufficient for CD 8 memory T cell differentiation . ^^^ Therefore , we conclude that full CD 8 memory differentiation requires only a single MHC class Ia chain , ruling out a requirement for MHC class Ib molecules in this process . . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Culture of PBMC with immobilized NKG2D specific mAb or NKG2D ligand MHC class 1 related protein A ( MICA ) induces the up regulation of CD 69 and CD 25 in NK and Vgamma 9 Vdelta2 but not in CD 8 T cells . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NKG2D , a prime activatory receptor on human NK , CD 8 ( + ) alphabeta and gammadelta cells , has a variety of ligands , which , despite sharing membership of the MHC class 1 structural club , display an array of unique features . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Mouse studies documented that CD 103 expression is required for efficient destruction of the graft renal tubules by CD 8 effectors directed to donor MHC 1 alloantigens . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
While much is known about the MHC class 1 restricted response following influenza virus infection of C57BL / 6 mice , with over 16 CD 8 epitopes identified to date , less is known about the MHC class 2 restricted response . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Tolerance induction absolutely required host expression of MHC class 1 , but was independent of CD 8 T cell dependent immunity . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Previous studies in mice indicated that G protein immunization resulted in antibody and Th 2 type response and failed to induce MHC 1 restricted CD 8 ( + ) T cell response . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
We confirm with this antibody the direct relationship between clone avidity and TCR affinity , and the moderate contribution of the CD 8 co receptor in the reinforcement of TCR MHC / peptide contact . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Analyzing expression of perforin , Runx 3 , and Thpok genes during positive selection reveals activation of CD 8 differentiation programs by MHC 2 signaled thymocytes . ^^^ This indicates that MHC 2 signals normally fail to impose CD 4 differentiation and further implies that the number of mature CD 8 single positive ( SP ) thymocytes greatly underestimates CD 8 lineage choice . ^^^ We next examined whether MHC 2 restricted CD4+ 8 thymocytes remain competent to initiate CD 8 lineage gene expression . ^^^ We conclude from these findings that MHC 2 signaled thymocytes remain competent to initiate CD 8 specific gene expression even after CD 8 down regulation and that CD 4 lineage differentiation is not fixed before the CD 4 SP stage . . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
CD 8 ( + ) T cell populations specific for the HLA A * 0101 restricted epitope NP ( 44 52 ) and the HLA A * 0201 restricted epitope M 1 ( 58 66 ) were identified by positive staining with tetramers of peptide major histocompatibility complexes ( MHC ) ( NP Tm and M 1 Tm , respectively ) . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Assembly of major histocompatibility complex ( MHC ) class 1 molecules in the endoplasmic reticulum is a highly coordinated process that results in abundant class I / peptide complexes at the cell surface for recognition by CD 8 ( + ) T cells and natural killer cells . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Second , we discuss mechanisms of cross presentation , the phenomenon whereby extracellular and luminal protein antigens can be processed by antigen presenting cells , particularly dendritic cells , and presented by MHC class 1 molecules to CD 8 ( + ) T cells . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
However , in some circumstances , antigens from the extracellular environment can be presented on MHC class 1 molecules and stimulate CD 8 ( + ) T cell immunity , a process termed cross presentation . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
CD 8 ( + ) T cells are activated by the presentation of antigenic peptide through MHC class 1 molecules . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
One such gene , ICP 47 , binds to the transporter associated with antigen presentation ( TAP ) 1 / 2 thereby preventing transport of viral peptides into the endoplasmic reticulum , loading of peptides onto nascent major histocompatibility complex ( MHC ) class 1 molecules , and presentation of peptides to CD 8 T cells . ^^^ This increase in virulence was due to inhibition of antigen presentation to CD 8 T cells , since these differences were not evident in MHC class 1 deficient mice or in mice in which CD 8 T cells were depleted . ^^^ Inhibition of MHC class 1 by the recombinant viruses did not impair the induction of the HSV specific CD 8 T cell response , indicating that cross presentation is the principal mechanism by which HSV specific CD 8 T cells are induced . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Cutting edge : differential self peptide / MHC requirement for maintaining CD 8 T cell function versus homeostatic proliferation . ^^^ Here , we show that memory CD 8 T cells retain the ability to respond to dendritic cell mediated stimulation after adoptive transfer into either TAP ( / ) ( MHC class 1 deficient ) or wild type mice . ^^^ Surprisingly , naive CD 8 T cells , which fail to undergo homeostatic proliferation and erode in number in the absence of MHC class 1 , also retain the ability to respond to dendritic cell mediated antigenic stimulation for at least 1 wk after transfer into TAP ( / ) mice . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
The targeted MZ DC were quite unique in their phenotype : CD11c ( + ) , CD8alpha ( ) , CD11b ( ) , B 220 ( ) , and expressing intermediate or low levels of MHC class 2 and DEC 205 . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Recent study has also shown that murine CD 8 alphaalpha binds to TL antigen , an MHC homologue , therefore acts as an immuno modulator . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
This review will detail the current advances in the knowledge of CD 8 Treg in the context of antigen specificity , phenotype , MHC restriction , mechanism of action , and priming . . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
MHC class 1 allo grafts can be directly rejected by recipient CD 8 T cells and be indirectly rejected by recipient CD 4 T cells . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Recent observations , using fluorescent tetramers of the MHC class Ib molecule TL , link transient expression of CD8alphaalpha homodimers on expanding primary effector CD 8 T cells to the generation of memory cells . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
We have used major histocompatibility complex ( MHC ) class I / peptide multimers to monitor the expansion of antigen specific CD 8 T lymphocytes from whole blood , exploring the effect of antigenic peptide dose , IL 2 , IL 7 and IL 15 concentrations on the magnitude and functional characteristics of the antigen specific CD 8 ( + ) T cells generated . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Targeting of PD 1 in several fully MHC mismatched models accelerated rejection , whereas targeting of BTLA unexpectedly enhanced PD 1 induction by alloreactive CD 4 and CD 8 T cells and prolonged allograft survival . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
CD 8 T cell populations restricted by H 2 M3 MHC class Ib molecules expand rapidly during primary Listeria monocytogenes infection but only minimally upon reinfection . ^^^ In contrast , CD 8 T cells restricted by MHC class Ia molecules undergo more delayed expansion during primary infection but rapid and robust expansion following reinfection . ^^^ In this study we demonstrate that primary H 2 M3 restricted CD 8 T cell responses are unaffected by the frequency of naive MHC class Ia restricted T cells during L . monocytogenes infection . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
EBV specific CD 8 ( + ) T lymphocytes were detected in CSF and peripheral blood using major histocompatibility complex ( MHC ) class 1 multimers loaded with EBV derived peptides . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
To understand the impact of AAV capsid specific CD 8 ( + ) T cells on AAV mediated gene transfer , we identified CD 8 ( + ) T cell epitopes for AAV 2 and AAV 8 capsid in C57BL / 6 ( H 2 ( b ) MHC haplotype ) and BALB / c ( H 2 ( d ) MHC haplotype ) mice . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Loss of CD 8 and TCR binding to Class 1 MHC ligands following T cell activation . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Proteins show drastic discrepancies in their contribution to the collection of self peptides that shape the repertoire of CD 8 T cells ( MHC 1 self immunopeptidome ) . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Furthermore , quantitation of the CD 8 T cell response against one of these epitopes ( residues 261 270 from the phosphoprotein ) by MHC class 1 pentamer staining and by in vitro stimulation followed by intracellular IFN gamma and TNF alpha staining indicated that the majority of pulmonary CD 8 specific for the P 261 epitope were deficient in cytokine production . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Stimulation of autologous T cells by the fusion cells generated with autologous dendritic cells ( HLA A 2 ( + ) and / or HLA A 24 ( + ) ) and allogeneic COLM 6 resulted in MHC class 1 and MHC class 2 restricted proliferation of CD 4 ( + ) and CD 8 ( + ) T cells , high levels of IFN gamma production in both CD 4 ( + ) and CD 8 ( + ) T cells , and the simultaneous induction of CEA and MUC 1 specific CTL responses restricted by HLA A 2 and / or HLA A 24 . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
CD 8 T cells contribute to protection against S . enterica serovar Typhimurium in mice , but little is known about the specificity and major histocompatibility complex ( MHC ) restriction of the response . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
To test the efficacy of vaccination with DC+B16 CD 44 , mice were vaccinated subcutaneously Lymphocytes from mice vaccinated with DC+B16 CD 44 produced IFN gamma in response to B 16 melanoma lysates as well as an MHC class 1 restricted B 16 melanoma associated peptide , indicating B 16 specific CD 8 T cell activation . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
These fusion cells expressed major histocompatibility complex ( MHC ) class 1 and MHC class 2 , CD 86 , CD11c and CD8alpha . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
To clarify this mechanism , we fed ovalbumin ( OVA ) to T cell receptor transgenic ( TCR Tg ) mice with OVA specific MHC class 2 restricted TCR and found that the cytotoxic activity of IELs was increased against both NK and LAK target cells , but notably reduced after depleting CD 8 + IELs . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Perforin expression can define CD 8 positive lymphocyte subsets in pigs allowing phenotypic and functional analysis of natural killer , cytotoxic T , natural killer T and MHC un restricted cytotoxic T cells . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Cross presentation is the process whereby bone marrow derived antigen presenting cells acquire , process and present exogenous antigen as peptides bound to MHC class 1 molecules to CD 8 ( + ) T cells . ^^^ Professional antigen presenting cells acquire cell associated antigen predominantly in the form of protein , then process and present antigenic peptides on their surface MHC class 1 molecules via several mechanisms and efficiently cross prime nave CD 8 ( + ) T cells in vivo . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
The PT ( n = 28 ) and its LNs were analyzed by EBER RNA in situ hybridization and by immunohistochemistry for MHC class 1 and 2 expression , for CD 3 , CD 8 , CD 4 , CD 20 , CD 56 , CD 83 , and Granzyme B ( GzB ) expression . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
CD 8 T cell mediated lung damage in response to the extracellular pathogen pneumocystis is dependent on MHC class 1 expression by radiation resistant lung cells . ^^^ How this extracellular pathogen elicits a CD 8 T cell response is not clear , and it was the aim of our study to determine the Ag specificity of the recruited CD 8 T cells and to determine whether MHC class 1 ( MHC 1 ) expression was necessary to initiate lung damage . ^^^ However , CD 8 T cell mediated lung damage is Ag specific is induced by a MHC 1 expressing nonbone marrow derived cell in the lung and is dependent on the continued presence of live Pneumocystis . . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Major histocompatibility complex ( MHC ) class 1 molecules present thousands of peptides to allow CD 8 ( + ) T cells to detect abnormal intracellular proteins . ^^^ We found that loss of ERAAP disrupted the generation of naturally processed peptides in the endoplasmic reticulum , decreased the stability of peptide MHC class 1 complexes and diminished CD 8 ( + ) T cell responses . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Structural basis of the differential stability and receptor specificity of H 2Db in complex with murine versus human beta 2 microglobulin . beta ( 2 ) Microglobulin ( beta ( 2 ) m ) is non covalently linked to the major histocompatibility complex ( MHC ) class 1 heavy chain and interacts with CD 8 and Ly 49 receptors . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
We demonstrate using the MHC class 1 restricted 2C TCR and MHC class 2 restricted AND TCR transgenic systems that TNFR 2 regulates the threshold for clonal expansion of CD 4 and CD 8 T cell subsets in response to cognate Ag . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
By immunohistochemical examination , the phenotypes of NP cells were determined as CD 1 , CD 4 , CD 5 , CD8alpha , sIgM+ , lambda light chain+ , B B4+ , MHC class II+ in both case . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
In vitro , MAPCs expressed low levels of major histocompatibility complex ( MHC ) antigens , failed to stimulate CD 4 ( + ) and CD 8 ( + ) T cell alloresponses , and were targets of NK cytolysis . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
The effector CD 8 ( + ) T cells recognize major histocompatibility complex ( MHC ) class 1 binding altered self peptides expressed in tumour cells . ^^^ Coimmunization of mice with Peptide 25 and ovalbumin ( OVA ) or Peptide 25 and B 16 melanoma peptide [ tyrosinase related protein 2 ( TRP 2 ) ] for MHC class 1 led to a profound increase in CD 8 ( + ) T cells specific for OVA and TRP 2 peptides , respectively . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
CD 8 T cells , like CD 4 T cells , are triggered by multivalent engagement of TCRs by MHC peptide ligands but not by monovalent engagement . ^^^ In determining the reactivity of CD 8 T cells to soluble activating class 1 MHC peptide complexes , a complicating phenomenon had been observed whereby peptide from soluble complexes was loaded onto cell surface MHCs on the T cells and re presented to other T cells , clouding the true valency requirement for activation . ^^^ This study uses soluble allogeneic class 1 MHC peptide monomers and oligomers to stimulate murine CD 8 T cells without the possible complication of peptide re presentation . ^^^ The results show that MHC class 1 monomers bind to , but do not activate , CD 8 T cells whether the cells are in solution or adhered to a surface . ^^^ Dimeric engagement is necessary and sufficient to stimulate downstream activation processes including TCR down regulation , Zap 70 phosphorylation , and CD 25 and CD 69 up regulation , even in T cells that do not express the MHC coreceptor CD 8 . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
DESIGN AND METHODS : The frequency and the functional phenotype of the anti ALK CD 8 precursor repertoire in freshly isolated peripheral blood mononuclear cells ( PBMC ) from healthy donors and ALK positive patients were determined by major histocompatibility complex ( MHC ) / tetrameric analyses . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Differences in T cell receptor ( TCR ) signaling initiated by interactions among TCRs , coreceptors , and self peptide MHC complexes determine the outcome of CD 4 versus CD 8 lineage of T cell differentiation . ^^^ Thus , expression of two TCRs by a single thymocyte , TCR recognition of multiple MHC molecules , and heterogeneity of TCR , coreceptors , and peptide MHC interactions in the thymus all contribute to the outcome of CD 4 versus CD 8 lineage development . . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
The generation of CD 4 and CD 8 alphabeta T cell lineages from CD4+ CD8+ double positive ( DP ) thymocyte precursors is a complex process initiated by engagement of major histocompatibility complex ( MHC ) by T cell receptor ( TCR ) and coreceptor . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
A paper in this issue of the European Journal of Immunology identifies a novel type of induced murine CD 8 ( + ) Treg that arise in TCR HY transgenic female mice after injection of MHC class 1 tetramers loaded with HY peptide . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Re evaluating the generation of a `` proteasome independent ' ' MHC class 1 restricted CD 8 T cell epitope . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Given the particularly high frequency of alloreactive T cells in the peripheral immune system , we used numerous MHC class 1 multimers to directly visualize and isolate viral and tumor Ag specific alloreactive CD 8 T cells . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
High concentrations of mononuclear cells are present in this blister fluid , principally CD 8 T lymphocytes that may exhibit a drug specific MHC class 1 restricted cytotoxicity against autologous cells . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Several types of CD 4 ( + ) MHC class 2 restricted Treg populations have been characterized , but the biology of CD 8 ( + ) , MHC class 1 restricted Tregs is less understood . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Here we show the identification of five candidate vaccine antigens that are the targets of MHC class 1 restricted CD 8 ( + ) CTL from immune cattle . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Here , we demonstrate that inclusion of Brefeldin A ( BfA ) , an agent reported to block MHC class 1 peptide complex turnover on the cell surface , during DC isolation from transgenic RIP mOVA mice facilitated activation and proliferation of nave OVA specific CD 8 ( + ) T cells in vitro . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
The MHC class 1 related ligands of the immunoreceptor NKG2D are frequently expressed by tumor cells and stimulate tumor immunity mediated by CD 8 T cells and natural killer ( NK ) cells . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Beta 2 microglobulin ( beta2m ) is non covalently linked to the major histocompatibility ( MHC ) class 1 heavy chain and interacts with CD 8 and Ly 49 receptors . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
In principle , a series of distinct class 1 MHC peptide tetramers , each labelled with a different fluorochrome , would allow to simultaneously enumerate as many unique antigen specific CD 8 ( + ) T cells . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Soluble MHC peptide ( pMHC ) complexes induce intracellular calcium mobilization , diverse phosphorylation events , and death of CD8+ CTL , given that they are at least dimeric and co engage CD 8 . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Their cell surface stained positive for the helper / inducer lymphocyte markers , T cell markers , CD 45 , Thy 1 , asialo GM 1 and FcR , but negative for B cell markers , such as membrane type IgM , CD 8 and MHC class 2 . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
We do not observe hyperproliferation of T cells lacking only one of these subunits , and thus hyperproliferation is independent of either reduced MHC class 1 expression in LMP 7 ( / ) mice or reduced CD 8 ( + ) T cell numbers in MECL 1 ( / ) mice . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
The human major histocompatibility complex ( MHC ) class 1 chain related gene A ( MICA ) maps 46 kb centromeric to HLA B and is highly polymorphic ; it encodes a stress inducible protein which functions as a ligand for the NKG2D / DAP10 complex to activate natural killer ( NK ) cells , gammadelta T cells , and CD 8 ( + ) T cells . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Clonal expansion of antigen specific CD 8 ( + ) T cells in the spleen was measured at 5 , 7 , 9 and 14 d post challenge using a class 1 MHC tetramer loaded with the immunodominant peptide from this pathogen ( i . e . ^^^ To our knowledge this is the first time MHC tetramers have been used to investigate the influence of n 3 PUFA on in vivo CD 8 ( + ) T cell proliferation . . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
We report that natural killer receptors ( NKR ) for major histocompatibility complex ( MHC ) class 1 molecules ( MHC NKR ) , the inhibitory killer immunoglobulin like receptors ( KIR ) , and the CD94 / NKG2A receptor are present on a small proportion of CD 8 T cells in cord blood . ^^^ CD 8 T cells expressing MHC NKR were present at similar levels in cord blood from preterm and term infants , and it is important that their presence was independent of placental pathology or infection . ^^^ Cord blood CD 8 T cells expressing MHC NKR were relatively homogeneous and entirely CD27+ , mostly CC chemokine receptor 7 and granzyme B , with a majority being CD45RA+ and with no evidence for a skewed distribution of T cell receptor Vbeta when tested in KIR+ cells . ^^^ This contrasted with adult blood , which was more heterogeneous , and where a majority of CD 8 T cells expressing MHC NKR was CD 27 and granzyme B+ . ^^^ These data suggest that cord blood CD 8 T cells expressing MHC NKR are a unique subset of cells , distinct from those in adult blood , and may represent a less differentiated population . . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
To further elucidate the immunopathogenesis , we investigated muscles of 53 dogs with MMM , 32 dogs with polymyositis ( PM ) , and 4 dogs suffering from both , with regard to the presence and location of CD 4 ( + ) and CD 8 ( + ) T cells , B cells , macrophages , major histocompatibility complex ( MHC ) class 1 and class 2 antigens , and autoantibodies . ^^^ CD 8 ( + ) T cells were found in MMM ( 91 % ) and PM ( 75 % ) , mostly paralleled ( 68 % and 61 % ) by enhanced expression of MHC class 1 antigen on muscle fibers . ^^^ Since MHC class 1 antigen and CD 8 ( + ) T cells were detected in the presence of CD 4 ( + ) T cells , regardless of antimuscular antibodies , we consider MMM and PM in the dog as a CD 8 ( + ) T cell mediated immunopathological disease that initiates muscle fiber destruction and leads to production of myosin autoantibodies . . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
On day 15 , there were similar numbers of all major subsets except B cells in the stroma , while on the endothelium macrophages , MHC class 2 ( + ) cells and CD 8 ( + ) cells predominated ( p < 0 . 001 CD 4 ( + ) vs CD 8 ( + ) cells ) . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
The numbers of cells expressing CD 2 , CD 4 , CD 8 , and CD 172 ( the 74 22 15 ( SWC 3 ) antibody is now known to be specific for CD 172 ) , MHC class 2 , and CD 25 were quantified using immunohistochemistry . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
We performed immunohistochemical stains for CD 3 , CD 4 , CD 8 , MHC 1 , MHC 2 , CD1a , HMB 45 , MART 1 , tyrosinase , bcl 2 , p 53 , and Ki 67 ( mib 1 ) . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
In a parallel approach , incorporating the results of proteasome mediated digestion of 5T4 derived 35 mer peptides and the potential high affinity epitopes predicted by a computer based algorithm , we identified 8 putative HLA A * 0201 presented CD 8 MHC class 1 epitopes of 5T4 antigen . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
CD11c ( + ) / CD11b ( + ) / CD205 ( mod ) / CD8alpha ( ) DC that captured liposomes were activated and presented this form of antigen in an MHC class 1 and class 2 restricted manner . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Effective control of chronic gamma herpesvirus infection by unconventional MHC Class Ia independent CD 8 T cells . ^^^ Control of virus infection is mediated in part by major histocompatibility complex ( MHC ) Class Ia presentation of viral peptides to conventional CD 8 T cells . ^^^ Although important , the absolute requirement for MHC Class Ia dependent CD 8 T cells for control of chronic virus infection has not been formally demonstrated . ^^^ We show here that mice lacking MHC Class Ia molecules ( K ( b / ) xD ( b / ) mice ) effectively control chronic gamma herpesvirus 68 ( gammaHV 68 ) infection via a robust expansion of beta 2 microglobulin ( beta 2 m ) dependent , but CD1d independent , unconventional CD 8 T cells . ^^^ This is the first demonstration to our knowledge that beta 2 m dependent , but Class Ia independent , unconventional CD 8 T cells can efficiently control chronic virus infection , implicating a role for beta 2 n dependent non classical MHC molecules in control of chronic viral infection . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Impact of CD 8 MHC class 1 interaction in detection and sorting efficiencies of antigen specific T cells using MHC class I / peptide multimers : contribution of pMHC valency . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Monitoring of CD 8 ( + ) T cells that specifically recognized a dominant MHC class 1 her 2 / neu peptide showed a dramatic increase in tetramer specific CD 8 ( + ) T cells after the first treatment ; however , continuous therapy resulted in the loss of this population . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
The sialyltransferase ST3Gal 1 is not required for regulation of CD 8 class 1 MHC binding during T cell development . ^^^ The CD 8 coreceptor plays a crucial role in thymocyte and T cell sensitivity by binding to class 1 MHC and recruiting downstream signaling molecules to the TCR . ^^^ Previous studies reported considerable changes in TCR independent CD8 / class 1 MHC binding ( i . e . , CD 8 noncognate interactions ) during T cell development , changes that correlated with altered glycosylation of surface molecules . ^^^ In contrast to this model , however , we report in this study that ST3Gal 1 deficiency had no detectable impact on CD 8 noncognate binding to multimeric peptide / MHC class 1 ligands at any stage of thymocyte development . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
CD 8 ( + ) T cells respond to short peptides bound to MHC class 1 molecules . ^^^ Although most antigenic proteins contain many sequences that could bind to MHC class 1 , few of these peptides actually stimulate CD 8 ( + ) T cell responses . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Host MHC class II+ antigen presenting cells and CD 4 cells are required for CD 8 mediated graft versus leukemia responses following delayed donor leukocyte infusions . ^^^ CD 8 ( + ) T cell dependent GVL responses against an MHC class 2 negative tumor following delayed DLI require CD 4 ( + ) T cell help and are reduced significantly when host APCs lack MHC class 2 expression . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
The CD 8 ( + ) DC are unique in that they can present exogenous antigens on their MHC class 1 molecules , a process known as cross presentation . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Here , evidence is presented that the CD 122 ( lo ) subset of MP CD 8 ( + ) cells is controlled largely by major histocompatibility complex ( MHC ) class 1 molecules . ^^^ Unlike CD 122 ( hi ) CD 8 ( + ) cells , CD 122 ( lo ) MP CD 8 ( + ) cells survive poorly after transfer to MHC 1 ( / ) hosts and cease to proliferate . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Interaction of CD 8 ( CD8alphaalpha or CD8alphabeta ) with the peptide major histocompatibility complex ( MHC ) class 1 ( pMHCI ) is critical for the development and function of cytolytic T cells . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
It does so by linking E 7 with the sorting signal of lysosome associated membrane protein 1 ( Sig / LAMP 1 ) to enhance the presentation of E 7 antigen to MHC class 1 restricted CD 8 ( + ) T cells , as well as to MHC class 2 restricted CD 4 ( + ) T cells . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Unexpectedly , a small number of functional CD 8 single positive thymocytes expressing the Tg MHC class 2 restricted TCR exists in mutant mice . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
CD 8 alpha alpha mediated intraepithelial lymphocyte snatching of thymic leukemia MHC class Ib molecules in vitro and in vivo . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
When complexed with MHC class 1 restricted specific peptide , vesicles from DC sonicates generate strong responses by purified nave CD 8 ( + ) cells in vitro in the absence of normal antigen presenting cells and can also efficiently prime T cells for tumor rejection in vivo . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Here we show that after skin infection with herpes simplex virus ( HSV ) , cytotoxic T lymphocyte ( CTL ) activation required MHC class 1 restricted presentation by nonmigratory CD 8 ( + ) DCs rather than skin derived DCs . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
To model DC specific T cell competition , normal mice were injected with one or two T cell receptor transgenic CD 8 T cell populations , each with high affinity for different peptides presented by different class 1 MHC complexes . ^^^ Thus , some aspect of DCs other than peptide / MHC complexes is limiting in the earliest stages of the CD 8 T cell response . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
In the current study , we used solid phase MHC class I / peptide monomers to cause activation induced cell death ( AICD ) of previously activated CD 8 T cells in an Ag specific manner . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Mapping the binding site on CD 8 beta for MHC class 1 reveals mutants with enhanced binding . ^^^ We also addressed the question of the orientation of CD8alphabeta with MHC class 1 using CD8alpha mutants expressed as a heterodimer with wild type CD8alpha or CD8beta . ^^^ The partial rescuing of binding with wild type CD8beta compared with wild type CD8alpha is consistent with models in which either the topology of CD8alphaalpha and CD8alphabeta binding to MHC class 1 is different or CD8alphabeta is capable of binding in both the T cell membrane proximal and distal positions . . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
To investigate the role of PD 1 in a chronic human viral infection , we examined PD 1 expression on human immunodeficiency virus ( HIV ) specific CD 8 T cells in 71 clade C infected people who were naive to anti HIV treatments , using ten major histocompatibility complex ( MHC ) class 1 tetramers specific for frequently targeted epitopes . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
Strikingly , the mutant thymocytes developed into CD 4 ( + ) and CD 8 ( + ) lineage T cells independent of the major histocompatibility complex ( MHC ) , indicating that the CD 4 ( + ) and CD 8 ( + ) lineage development programs are constitutively active in the absence of c Cbl and Cbl b . ^^^ Thus , our data indicate that Cbl proteins play a critical role in establishing the MHC dependent CD 4 ( + ) and CD 8 ( + ) T cell development programs . ^^^
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13747 and P01732	Pubmed	SVM Score :0.0
NA