| Interacting proteins: P13686 and P10451 |
Pubmed |
SVM Score :0.0 |
| To address the physiologic responses associated with these effects on bone mineral density ( BMD ) , we assessed mRNA transcripts reflecting activities of osteoblasts ( type 1 collagen , osteocalcin , osteopontin , and alkaline phosphatase ) , osteoclasts [ tartrate resistant acid phosphatase ( TRAP ) ] , and cell proliferation ( histone H 4 ) in the spine and femur of these rats . ^^^ |
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| Interacting proteins: P13686 and P10451 |
Pubmed |
SVM Score :0.0 |
| The tartrate resistant acid phosphatase ( TRAP ) of skeletal osteoclasts was found to partially dephosphorylate the bone matrix phosphoproteins osteopontin ( OPN ) and bone sialoprotein ( BSP ) . ^^^ TRAP also partially dephosphorylated metabolically [ 32P ] PO 4 labeled OPN as well as BSP , whereas comparable amounts of either alkaline phosphatase or prostatic acid phosphatase , at their respective pH optima , were ineffective , indicating a certain preference of TRAP for these phosphoprotein substrates . ^^^ |
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| Interacting proteins: P13686 and P10451 |
Pubmed |
SVM Score :0.0 |
| Adjacent clusters of mononuclear cells were TRAP and NSE+ or were active for both enzymes ; these cells demonstrated variable expression of osteopontin mRNA . ^^^ In the inflammatory connective tissues , abundant macrophage like cells ( NSE+ / TRAP ) did not express osteopontin mRNA . ^^^ Therefore , cells involved in the remodeling ( resorption ) of bone or the removal of bone debris , together with their immediate precursors , switch from being NSE+ / TRAP to NSE / TRAP+ cells that express osteopontin mRNA . ^^^ |
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| Interacting proteins: P13686 and P10451 |
Pubmed |
SVM Score :0.0 |
| Osteopontin mRNA was expressed in osteoclast with tartrate resistant acid phosphatase ( TRAP ) positivity within resorption lacunae . ^^^ |
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| Interacting proteins: P13686 and P10451 |
Pubmed |
SVM Score :0.0 |
| The isolated cells demonstrated a number of phenotypic characteristics of osteoclasts : positive tartrate resistant acid phosphatase ( TRAP ) activity , reactivity with human osteoclast selective antibodies , expression of calcitonin receptors , cathepsin K ( a novel osteoclast selective cysteine proteinase ) mRNA , and osteopontin mRNA and protein . ^^^ In contrast , isolated peripheral blood monocytes were negative for TRAP activity and osteopontin expression and , unlike the osteoclastoma derived cells , demonstrated strong nonspecific esterase activity . ^^^ |
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| Interacting proteins: P13686 and P10451 |
Pubmed |
SVM Score :0.0 |
| In the same cultures , cells expressing alkaline phosphatase ( AP ) or tartrate resistant acid phosphatase ( TRAP ) , phenotypic markers for osteoblastic and osteoclast like cells , respectively , appeared subsequent to the appearance of the osteopontin positive cells . ^^^ By means of a combination of in situ hybridization and histostaining , it was shown that the osteopontin mRNA was localized in 30 50 % of the AP positive or the TRAP positive , as well as in nonspecific esterase ( NSE ) positive , cells . ^^^ |
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| Interacting proteins: P13686 and P10451 |
Pubmed |
SVM Score :0.0 |
| The addition of an OPN antisense oligomer ( 5 ' AAT CAC TGC CAA TCT CAT 3 ' ) at the start of the co culture period decreased the number of TRAP ( + ) cells present after 7 d ( 30 . 3 + / 3 . 4 vs 56 . 9 + / 12 . 4 ) , and the ratio of mononuclear and multinucleated cells was changed ( 77 . 6 : 23 . 2 vs 60 . 8 : 39 . 3 ) . ^^^ |
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| Interacting proteins: P13686 and P10451 |
Pubmed |
SVM Score :0.0 |
| The cellular distribution of osteoclast integrin subunits alpha ( 5 ) and beta ( 3 ) , the tissue distribution , and level of the apparent ligand osteopontin ( OPN ) as well as of the putative regulatory enzyme tartrate resistant acid phosphatase ( TRAP ) were studied along with the intracellular distribution of the activation marker c src in osteopetrotic ia / ia ( incisors absent ) mutant rats and their normal littermates . ^^^ |
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| Interacting proteins: P13686 and P10451 |
Pubmed |
SVM Score :0.0 |
| As markers related to osteoclasts , we selected cathepsin K , carbonic anhydrase ( CA ) 2 , vacuolar H ( + ) ATPase ( 5 ATPase ) , vitronectin receptor ( VNR ) , tartrate resistant acid phosphatase ( TRAP ) , osteopontin ( OPN ) , galectin 3 , c src , c fos , and c fms . ^^^ Of these mRNAs , cathepsin K , CA 2 , 5 ATPase , VNR , TRAP , OPN , and c fms mRNAs were expressed at higher levels in the osteoclast like cells than those in monocytes cultured with control antibody . ^^^ |
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| Interacting proteins: P13686 and P10451 |
Pubmed |
SVM Score :0.0 |
| The TMC 16 cell line weakly expressed cath K , TRAP , and OPN , suggesting that the TMC 16 cell line is immortalized at a stage slightly differentiated from MDBM cells . ^^^ |
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| Interacting proteins: P13686 and P10451 |
Pubmed |
SVM Score :0.0 |
| We demonstrated that the transcriptional expression of MT 1 MMP , MMP 9 , cathepsin K , and TRAP in highly enriched osteoclasts were regulated by different matrix proteins that bind to integrin on osteoclast , such as collagen type 1 ( CoI ) , fibronectin ( FN ) , vitronectin ( VN ) , osteopontin ( OPN ) , and ivory . ^^^ |
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| Interacting proteins: P13686 and P10451 |
Pubmed |
SVM Score :0.0 |
| To obtain insight into the cellular mechanism underlying the phenomena observed in osteopontin deficient bone , we investigated the number of tartrate resistant acid phosphatase ( TRAP ) positive cells in the bones subjected to PTH treatment in cultures . ^^^ The number of TRAP positive cells was increased significantly by PTH in wild type bone ; however , no such PTH induced increase in TRAP positive cells was observed in osteopontin deficient bones . ^^^ |
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| Interacting proteins: P13686 and P10451 |
Pubmed |
SVM Score :0.0 |
| OPN was localized in two distinct cell types , i . e . , fibroblast like cells negative for CD 68 and tartrate resistant acid phosphatase ( TRAP ) and multinucleated macrophages positive for CD 68 and TRAP . ^^^ |
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| Interacting proteins: P13686 and P10451 |
Pubmed |
SVM Score :0.0 |
| All forms of OPN , including rOPN , significantly increased attachment of tartrate resistant acid phosphatase ( TRAP ) positive osteoclasts . ^^^ |
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| Interacting proteins: P13686 and P10451 |
Pubmed |
SVM Score :0.0 |
| Expressions of collagen alpha 1 ( 1 ) , osteocalcin , osteopontin mRNAs and TRAP in the surrounding tissue were monitored . ^^^ These cells expressed osteopontin mRNA intensively and some TRAP enzyme activity occasionally . . ^^^ |
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| Interacting proteins: P13686 and P10451 |
Pubmed |
SVM Score :0.0 |
| TRAP is able to degrade skeletal phosphoproteins including osteopontin , identical to the T cell cytokine , Eta 1 ; we thus propose that TRAP regulates the Eta 1 pathway common to the immune system and skeleton . ^^^ We compared the distribution of osteopontin and TRAP in sections of 18 day old embryonic mice by immunohistochemistry . ^^^ To determine whether gold compounds exert their effects by modification of TRAP activity , we examined the action of gold chloride and the prodrugs , aurothioglucose and aurothiomalate on the dephosphorylation of osteopontin by TRAP . ^^^ |
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| Interacting proteins: P13686 and P10451 |
Pubmed |
SVM Score :0.0 |
| Three human choriocarcinoma cell lines , namely JAR , BeWo , and JEG 3 , were treated with variants of OPN differing in the extent of phosphorylation following sequential dephosphorylation with tartrate resistant acid phosphatase ( TRAP ) , and their migratory response was measured . ^^^ Because both OPN and TRAP are expressed in the uterus during early pregnancy , it is conceivable that extracellular phosphatases such as TRAP may modify OPN charge state and thus modulate cell migration . . ^^^ |
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| Interacting proteins: P13686 and P10451 |
Pubmed |
SVM Score :0.0 |
| Specimens were harvested on days 4 , 7 , 14 , 28 and 56 after implantation , and were analyzed by tartrate resistant acid phosphatase ( TRAP ) staining , immunohistochemistry of the ED 1 protein as a marker of the phagocyte system , and in situ hybridization with digoxigenin labeled alpha 1 chain of type 1 procollagen ( COL1A1 ) , osteopontin and osteocalcin . beta TCP was resorbed in a chronological manner . ^^^ |
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| Interacting proteins: P13686 and P10451 |
Pubmed |
SVM Score :0.0 |
| The paraffin sections were subject to tartrate resistant acid phosphatase ( TRAP ) enzyme histochemistry and immunohistochemistry for alkaline phosphatase ( ALP ) , osteopontin ( OP ) and osteocalcin ( OC ) . ^^^ |
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| Interacting proteins: P13686 and P10451 |
Pubmed |
SVM Score :0.0 |
| Cells treated with TRAP were evaluated for cell proliferation , gene expression for osteocalcin ( OCN ) , osteopontin ( OPN ) , and bone sialoprotein ( BSP ) , and induction of mineral nodule formation . ^^^ Gene expression of OCN , OPN , and BSP in TRAP treated cementoblasts showed down regulation , up regulation , and no significant change , respectively , relative to vehicle control . ^^^ |
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| Interacting proteins: P13686 and P10451 |
Pubmed |
SVM Score :0.0 |
| Microscopic analyses showed an accumulation of osteopontin adjacent to actively resorbing osteoclasts of Acp 5 and LAP / Acp5 deficient mice . ^^^ The vacuoles in Acp 5 and LAP / Acp5 doubly deficient osteoclasts also contained crystallite like features , as well as osteopontin , suggesting that Acp 5 is important for processing of this protein . ^^^ We conclude that for several substrates LAP and Acp 5 can substitute for each other and that these acid phosphatases are essential for processing of non collagenous proteins , including osteopontin , by osteoclasts . . ^^^ |
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| Interacting proteins: P13686 and P10451 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P13686 and P10451 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13686 and P10451 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13686 and P10451 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13686 and P10451 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13686 and P10451 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13686 and P10451 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13686 and P10451 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13686 and P10451 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13686 and P10451 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13686 and P10451 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13686 and P10451 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13686 and P10451 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13686 and P10451 |
Pubmed |
SVM Score :0.0 |
| NA |
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