| The micromolar amounts of the factors and ribosomes are as follows : EF 1 alpha , 34 ; EF 2 , 5 . 2 ; eIF 2 , 1 . 5 ; eIF 3 , 0 . 7 ; eIF 4A , 3 . 0 , eIF 4F , 0 . 09 ; eIF ( iso ) 4F , 0 . 8 ; and 40 S ribosomal subunits , 3 . 2 . ^^^ The molar ratios of the factors to 40 S ribosomal subunits are approximately 11 : 1 for EF 1 alpha , 1 . 6 : 1 for EF 2 , 0 . 45 : 1 for eIF 2 , 0 . 2 : 1 for eIF 3 , 0 . 9 : 1 for eIF 4A , 0 . 03 : 1 for eIF 4F , and 0 . 25 : 1 for eIF ( iso ) 4F . ^^^ |
| We found that the vast majority of translation initiation factors ( eIF 2 , eIF2B , eIF 3 , eIF4A1 , eIF 5 and eIF5B ) , all three elongation factors ( eEF1A , eEF1B and eEF 2 ) and the termination factor eRF 1 are strictly excluded from nuclei . ^^^ |