| Interacting proteins: Q5T2W1 and P13569 |
Pubmed |
SVM Score :0.76224676 |
| To further study potential functional interactions between CFTR and gap junction channels , we have co expressed CFTR and connexin 45 ( Cx 45 ) in Xenopus oocytes and monitored junctional conductance and voltage sensitivity by dual voltage clamp electrophysiology . 0.76224676^^^ |
|
| Interacting proteins: Q5T2W1 and P13569 |
Pubmed |
SVM Score :0.0 |
| The 70 kDa protein was identified as CAP 70 ( CFTR associated protein of 70 kDa ) by an anti CAP 70 antibody and by two in vitro binding assays after cloning CAP 70 from rabbit duodenum and ileum . ^^^ |
|
| Interacting proteins: Q5T2W1 and P13569 |
Pubmed |
SVM Score :0.0 |
| We have identified a novel hydrophilic CFTR binding protein , CAP 70 , which is also concentrated on the apical surfaces . ^^^ CAP 70 consists of four PDZ domains , three of which are capable of binding to the CFTR C terminus . ^^^ Linking at least two CFTR molecules via cytoplasmic C terminal binding by either multivalent CAP 70 or a bivalent monoclonal antibody potentiates the CFTR chloride channel activity . ^^^ |
|
| Interacting proteins: Q5T2W1 and P13569 |
Pubmed |
SVM Score :0.0 |
| All cells tested demonstrated outwardly rectifying chloride channels by patch clamp , with some from non CF cells responsive to the catalytic subunit of cyclic AMP dependent protein kinase . ^^^ |
|
| Interacting proteins: Q5T2W1 and P13569 |
Pubmed |
SVM Score :0.0 |
| The apical chloride channels found with the patch clamp technique in NCF 3 and in the postcrisis cell line NCF3A have a conductance similar to that of chloride channels found earlier in normal and CF epithelial cells . ^^^ |
|
| Interacting proteins: Q5T2W1 and P13569 |
Pubmed |
SVM Score :0.0 |
| In excised membrane patches the conductances for CF and N Cl channels were larger at positive as compared to negative clamp voltages ( Vc ) : 74 + / 2 . 6 ( Vc greater than 0 ) and 47 + / 2 . 0 pS ( Vc less than 0 ) for CF ( n = 57 ) and 69 + / 3 . 6 ( Vc greater than 0 ) and 45 + / 2 . 3 pS ( Vc less than 0 ) for N ( n = 35 ) . ^^^ The immediate appearance ( within 1 s ) of Cl channels after excision was observed at positive ( n = 6 ) as well as at negative clamp voltage ( n = 13 ) . `` Excision activation ' ' of CF Cl channels was observed at low ( less than 10 ( 9 ) mol / l ) or high ( 10 ( 3 ) mol / l ) calcium activities on the cytosolic side of the excised patch . ^^^ |
|
| Interacting proteins: Q5T2W1 and P13569 |
Pubmed |
SVM Score :0.0 |
| Utilizing the patch clamp technique , we examined human cultured respiratory epithelial cells derived from patients with cystic fibrosis ( CF ) and normals individual ( N ) for the existence of and for the properties of K+ channels . ^^^ |
|
| Interacting proteins: Q5T2W1 and P13569 |
Pubmed |
SVM Score :0.0 |
| Indices of tissue glucose metabolic rate Rg ' and of the percentage of total glucose uptake incorporated into specific storage products ( Cf ) are derived from tissue analysis after bolus administration of 2 [ 3H ] deoxyglucose and [ 14C ] glucose during the plateau phase of the euglycemic clamp . ^^^ |
|
| Interacting proteins: Q5T2W1 and P13569 |
Pubmed |
SVM Score :0.0 |
| CFTR was expressed in airway and heterologous cells and studied under whole cell voltage clamp conditions , which permitted the intracellular NAD ( P ) + / NAD ( P ) H ratio to be varied independently of ATP concentration . ^^^ |
|
| Interacting proteins: Q5T2W1 and P13569 |
Pubmed |
SVM Score :0.0 |
| Analysis of the 27 exons of the CFTR gene using a GC clamp denaturing gradient gel electrophoresis ( DGGE ) assay has enabled us to identify over 96 % of the mutated alleles . ^^^ |
|
| Interacting proteins: Q5T2W1 and P13569 |
Pubmed |
SVM Score :0.0 |
| HCO 3 permeability was assessed from measurements of intracellular pH [ pHi ; from spectrofluorimetry of the pH sensitive dye 2 ' , 7 ' bis ( 2 carboxyethyl ) 5 ( and 6 ) carboxyfluorescein ] and of channel activity ( patch clamp ; cell attached and isolated , inside out patches ) on NIH 3T3 fibroblasts and C 127 mammary epithelial cells transfected with wild type CFTR ( WT CFTR ) or delta F 508 CFTR , and also on mock transfected cells . ^^^ |
|
| Interacting proteins: Q5T2W1 and P13569 |
Pubmed |
SVM Score :0.0 |
| In whole cell patch clamp recordings , induction of the cAMPdPK mutation inhibited anionic conductances indicative of the CFTR chloride channel , whereas purified catalytic subunit of cAMPdPK , added intracellularly , reversed the inhibition . ^^^ |
|
| Interacting proteins: Q5T2W1 and P13569 |
Pubmed |
SVM Score :0.0 |
| Whole cell patch clamp was used to look for cystic fibrosis transmembrane conductance regulator ( CFTR ) like chloride currents in calcium tolerant human cardiac myocytes . ^^^ |
|
| Interacting proteins: Q5T2W1 and P13569 |
Pubmed |
SVM Score :0.0 |
| To examine whether normal and mutant CFTRs function as chloride channels when they reside in the ER , the patch clamp technique was used to measure currents in the outer membrane of nuclei isolated from mammalian cells expressing CFTR . ^^^ |
|
| Interacting proteins: Q5T2W1 and P13569 |
Pubmed |
SVM Score :0.0 |
| By use of whole cell patch clamp recordings , we show that nitric oxide activates cystic fibrosis transmembrane conductance regulator CI currents in normal human cloned T cells by a cGMP dependent mechanism . ^^^ |
|
| Interacting proteins: Q5T2W1 and P13569 |
Pubmed |
SVM Score :0.0 |
| In an ongoing effort to identify these alleles , we have scanned the entire coding sequences of the CF gene using a GC clamp denaturing gradient gel electrophoresis assay in a sample of 57 chromosomes from patients of italian origin . ^^^ |
|
| Interacting proteins: Q5T2W1 and P13569 |
Pubmed |
SVM Score :0.0 |
| Whole cell patch clamp analysis revealed that milrinone generated chloride conductances with properties consistent with those of CFTR . ^^^ |
|
| Interacting proteins: Q5T2W1 and P13569 |
Pubmed |
SVM Score :0.0 |
| We detected receptor function as changes in currents carried by adenosine 3 ' , 5 ' cyclic monophosphate ( cAMP ) regulated chloride channels provided by the cystic fibrosis transmembrane conductance regulator ( CFTR ) and recorded by two electrode voltage clamp . ^^^ |
|
| Interacting proteins: Q5T2W1 and P13569 |
Pubmed |
SVM Score :0.0 |
| Concomitant with the appearance of Pgp and MDR phenotype ( drug resistance , reduced drug accumulation and increased drug efflux ) , CFTR levels and cAMP stimulated Cl conductances were markedly decreased compared to wild type HT 29 ( Pgp ) cells ( as shown using the whole cell patch clamp technique ) . ^^^ |
|
| Interacting proteins: Q5T2W1 and P13569 |
Pubmed |
SVM Score :0.0 |
| During the hyperglycemic clamp , the insulin sensitivity index was lower in CF IGT , but not CF NGT , compared with control values ( 6 . 66 + / 1 . 79 , 12 . 82 + / 1 . 61 , and 13 . 02 + / 1 . 78 mumol / kg . min / pmol . ^^^ During the hyperinsulinemic euglycemic clamp , insulin stimulated glucose disposal was significantly lower in CF IGT ( 45 . 68 + / 4 . 87 mumol / kg . min ) vs . ^^^ |
|
| Interacting proteins: Q5T2W1 and P13569 |
Pubmed |
SVM Score :0.0 |
| We have evaluated the metabolic clearance rate ( MCR ) of insulin and insulin sensitivity in 12 older patients with cystic fibrosis ( CF ) using the hyperinsulinemic euglycemic clamp method . ^^^ Glucose disposal rates ( M ) were similar in the two groups during the clamp ( CF = 7 . 28 + / 0 . 41 5 controls = 6 . 83 + / 0 . 60 mg / kg / min , P > . 5 ) , but the insulin sensitivity index , M / I 10 100 ( 1 = steady state insulin concentration ) , was markedly increased in the CF group ( CF = 17 . 62 + / 1 . 30 5 controls = 11 . 75 + / 0 . 71 , P < . 005 ) . ^^^ |
|
| Interacting proteins: Q5T2W1 and P13569 |
Pubmed |
SVM Score :0.0 |
| The aim of this study was to determine the ATP conductance of wild type CFTR channels stably expressed in Chinese hamster ovary cells using patch clamp techniques . ^^^ |
|
| Interacting proteins: Q5T2W1 and P13569 |
Pubmed |
SVM Score :0.0 |
| We have studied a sample of 39 CF patients of Tunisian origin and have used a GC clamp DGGE assay to scan the CFTR gene . ^^^ |
|
| Interacting proteins: Q5T2W1 and P13569 |
Pubmed |
SVM Score :0.0 |
| Southern hybridization and patch clamp analyses suggested that approximately 1 in 100 CF cells underwent a homologous replacement event that resulted in intact Cl transport . . ^^^ In addition , the functional activity of CFTR protein was determined by whole cell patch clamp . ^^^ |
|
| Interacting proteins: Q5T2W1 and P13569 |
Pubmed |
SVM Score :0.0 |
| This study used whole cell patch clamp and RNA in situ hybridization experiments to determine whether the cAMP activated C 1 current expressed in choroid plexus epithelial cells was carried by the cystic fibrosis transmembrane conductance regulator ( CFTR ) channel . 2 . ^^^ |
|
| Interacting proteins: Q5T2W1 and P13569 |
Pubmed |
SVM Score :0.0 |
| The role of the tyrosine kinase p60c src on the gating of the cystic fibrosis transmembrane conductance regulator ( CFTR ) chloride channel was investigated with the cell attached and excised patch clamp technique in conjunction with current noise analysis of recordings containing multiple channels per patch . ^^^ |
|
| Interacting proteins: Q5T2W1 and P13569 |
Pubmed |
SVM Score :0.0 |
| In these cells , CFTR was activated by membrane permeant analogs of cGMP or by the cGMP elevating hormone atrial natriuretic peptide as measured by 125I efflux assays and whole cell patch clamp analysis . ^^^ |
|
| Interacting proteins: Q5T2W1 and P13569 |
Pubmed |
SVM Score :0.0 |
| Permeation of cystic fibrosis transmembrane conductance regulator ( CFTR ) Cl channels by halide ions was studied in stably transfected Chinese hamster ovary cells by using the patch clamp technique . ^^^ |
|
| Interacting proteins: Q5T2W1 and P13569 |
Pubmed |
SVM Score :0.0 |
| Permeability of the cystic fibrosis transmembrane conductance regulator ( CFTR ) chloride channel to polyatomic anions of known dimensions was studied in stably transfected Chinese hamster ovary cells by using the patch clamp technique . ^^^ |
|
| Interacting proteins: Q5T2W1 and P13569 |
Pubmed |
SVM Score :0.0 |
| Using [ 1 C ] leucine and a three step hyperinsulinemic euglycemic clamp , we measured rates of leucine appearance in 29 adult CF patients and 18 matched control volunteers . ^^^ |
|
| Interacting proteins: Q5T2W1 and P13569 |
Pubmed |
SVM Score :0.0 |
| Two electrode voltage clamp recordings , 36Cl efflux assays , and whole cell patch clamp recordings were used to assay for the Cl channel function of CFTR and for its ability to regulate ORCCs . ^^^ |
|
| Interacting proteins: Q5T2W1 and P13569 |
Pubmed |
SVM Score :0.0 |
| The relationship between phosphorylation of the cystic fibrosis transmembrane conductance regulator ( CFTR ) chloride channel and its gating by nucleotides was examined using the patch clamp technique by comparing strongly phosphorylated wild type ( WT ) channels with weakly phosphorylated mutant channels lacking four ( 4SA ) or all ten ( 10SA ) dibasic consensus sequences for phosphorylation by protein kinase A ( PKA ) . 2 . ^^^ |
|
| Interacting proteins: Q5T2W1 and P13569 |
Pubmed |
SVM Score :0.0 |
| On the basis of their structure , we compared the ability of 35 xanthine derivatives to activate the cystic fibrosis transmembrane conductance regulator ( CFTR ) chloride channel stably expressed in chinese hamster ovary ( CHO ) cells using the cell attached patch clamp and iodide efflux techniques . 2 . ^^^ |
|
| Interacting proteins: Q5T2W1 and P13569 |
Pubmed |
SVM Score :0.0 |
| Patch clamp on the luminal membrane of exocrine gland acini from frog skin ( Rana esculenta ) reveals the presence of cystic fibrosis transmembrane conductance regulator like Cl channels activated by cyclic AMP . ^^^ |
|
| Interacting proteins: Q5T2W1 and P13569 |
Pubmed |
SVM Score :0.0 |
| The effects of individually mutating two adjacent threonine residues in the sixth membrane spanning region ( TM 6 ) of the cystic fibrosis transmembrane conductance regulator ( CFTR ) Cl channel on permeation properties were examined using patch clamp recording from mammalian cell lines stably expressing human CFTR . 2 . ^^^ |
|
| Interacting proteins: Q5T2W1 and P13569 |
Pubmed |
SVM Score :0.0 |
| To determine whether Paneth cells exhibit functional expression of cAMP activated Cl currents and molecular expression of the cystic fibrosis transmembrane conductance regulator ( CFTR ) , we applied whole cell patch clamp and single cell mRNA analysis by reverse transcription ( RT ) followed by polymerase chain reaction ( PCR ) amplification to single Paneth cells in crypts isolated from the guinea pig small intestine . 2 . ^^^ |
|
| Interacting proteins: Q5T2W1 and P13569 |
Pubmed |
SVM Score :0.0 |
| The effects of physiological substrates of multidrug resistance associated proteins ( MRPs ) on cystic fibrosis transmembrane conductance regulator ( CFTR ) Cl channel currents were examined using patch clamp recording from CFTR transfected mammalian cell lines . 2 . ^^^ |
|
| Interacting proteins: Q5T2W1 and P13569 |
Pubmed |
SVM Score :0.0 |
| Using [ 6 , 6 2H2 ] glucose and a three step hyperinsulinemic euglycemic clamp , we measured HGP in 29 adult CF subjects and 18 control volunteers . ^^^ |
|
| Interacting proteins: Q5T2W1 and P13569 |
Pubmed |
SVM Score :0.0 |
| Wild type and mutant CFTR chloride channels were expressed in Xenopus oocytes and CFTR currents measured using the two microelectrode voltage clamp . ^^^ |
|
| Interacting proteins: Q5T2W1 and P13569 |
Pubmed |
SVM Score :0.0 |
| Single and whole cell patch clamp recordings from CHO cells confirm that CFTR is the only channel activated by the drugs . ^^^ |
|
| Interacting proteins: Q5T2W1 and P13569 |
Pubmed |
SVM Score :0.0 |
| This mutation , named D513G ( A > G at position 1670 ) , has been found in one of 83 patients with CBAVD from France , the analysis of exon 10 using a chemical clamp DGGE assay allowed us to identify three CF mutations AEF 508 ( 37 / 166 ; 22 % ) , AE 1507 ( 1 / 166 ; 0 / 6 % ) and D513G ( 1 / 166 ; 0 . 6 % ) , and two variants M470V and E528E ( 1716 G > A ) . ^^^ |
|
| Interacting proteins: Q5T2W1 and P13569 |
Pubmed |
SVM Score :0.0 |
| To elucidate the complex nature of this interaction , CFTR constructs were co expressed with ROMK 2 in Xenopus oocytes , and two microelectrode voltage clamp experiments were performed . ^^^ |
|
| Interacting proteins: Q5T2W1 and P13569 |
Pubmed |
SVM Score :0.0 |
| Whole cell patch clamp of Calu 3 cells shows that Ht 31 peptide reduces cAMP stimulated CFTR Cl ( ) current , but Ht31P does not . ^^^ |
|
| Interacting proteins: Q5T2W1 and P13569 |
Pubmed |
SVM Score :0.0 |
| Here 1 show , using patch clamp recording from CFTR transfected baby hamster kidney cell lines , that the cis unsaturated fatty acid arachidonic acid also inhibits CFTR Cl currents when applied to the cytoplasmic face of excised membrane patches . ^^^ |
|
| Interacting proteins: Q5T2W1 and P13569 |
Pubmed |
SVM Score :0.0 |
| CFTR channel activity , assayed using two microelectrode voltage clamp and excised patch recordings , provided a sensitive measure of successful assembly of each pair of channel segments as the sever point was systematically shifted along the primary sequence . ^^^ |
|
| Interacting proteins: Q5T2W1 and P13569 |
Pubmed |
SVM Score :0.0 |
| We used control ( PANC 1 ) and CFTR deficient ( CFPAC 1 ; DeltaF 508 mutation ) cell lines and measured HCO 3 extrusion by the rate of recovery of intracellular pH after an alkaline load and recorded whole cell membrane currents using patch clamp techniques . 1 ) In PANC 1 cells , cAMP causes parallel activation of Cl channels and of HCO 3 extrusion by DIDS sensitive and Na+ independent Cl / HCO3 exchange , both effects being inhibited by Cl channel blockers NPPB and glibenclamide . 2 ) In CFPAC 1 cells , cAMP fails to stimulate Cl / HCO3 exchange and Cl channels , except after promoting surface expression of DeltaF 508 CFTR by glycerol treatment . ^^^ |
|
| Interacting proteins: Q5T2W1 and P13569 |
Pubmed |
SVM Score :0.0 |
| Here it is shown , using patch clamp recording from CFTR transfected mammalian cell lines , that the fatty acids butyrate and 4 phenylbutyrate cause a voltage dependent block of CFTR Cl ( ) currents when applied to the cytoplasmic face of membrane patches , with apparent K ( d ) s ( at 0 mV ) of 29 . 6 mM for butyrate and 6 . 6 mM for 4 phenylbutyrate . ^^^ |
|
| Interacting proteins: Q5T2W1 and P13569 |
Pubmed |
SVM Score :0.0 |
| Anion binding within the pores of wild type and mutant cystic fibrosis transmembrane conductance regulator ( CFTR ) Cl channels , expressed in two different mammalian cell lines , was assayed using patch clamp recording . ^^^ |
|
| Interacting proteins: Q5T2W1 and P13569 |
Pubmed |
SVM Score :0.0 |
| Patch clamp analysis revealed that PMA activated a Cl ( ) current with the typical biophysical characteristics of swelling activated current and not of CFTR . . ^^^ |
|
| Interacting proteins: Q5T2W1 and P13569 |
Pubmed |
SVM Score :0.0 |
| Here 1 show , using patch clamp recording from CFTR transfected mammalian cell lines , that under steady state conditions neither SCN conductance nor SCN permeability show anomalous mole fraction behaviour . ^^^ |
|
| Interacting proteins: Q5T2W1 and P13569 |
Pubmed |
SVM Score :0.0 |
| Using two electrode voltage clamp , we tested for changes in N associated with activation of CFTR in Xenopus oocytes using a cysteine substituted construct ( R334C CFTR ) that can be modified by externally applied , impermeant thiol reagents like [ 2 ( trimethylammonium ) ethyl ] methanethiosulfonate bromide ( MTSET+ ) . ^^^ |
|
| Interacting proteins: Q5T2W1 and P13569 |
Pubmed |
SVM Score :0.0 |
| We demonstrated , using iodide efflux , whole cell patch clamp , and short circuit recordings , that 5 butyl 6 hydroxy 10 chlorobenzo [ c ] quinolizinium chloride ( MPB 91 ) restored the activity of G551D CFTR ( EC ( 50 ) = 85 microM ) and activated CFTR in Calu 3 cells ( EC ( 50 ) = 47 microM ) . ^^^ |
|
| Interacting proteins: Q5T2W1 and P13569 |
Pubmed |
SVM Score :0.0 |
| We have used patch clamp recordings from native pancreatic duct cells to study HCO ( 3 ) ( ) permeation and interaction with CFTR . ^^^ |
|
| Interacting proteins: Q5T2W1 and P13569 |
Pubmed |
SVM Score :0.0 |
| We have studied the effects of point mutations throughout the sixth transmembrane ( TM 6 ) region of CFTR on channel block by , and permeability of , the highly lyotropic Au ( CN ) ( 2 ) ( ) anion , using patch clamp recording from transiently transfected baby hamster kidney cells . ^^^ |
|
| Interacting proteins: Q5T2W1 and P13569 |
Pubmed |
SVM Score :0.0 |
| Patch clamp electrophysiology of morulae and blastocysts demonstrated typical CFTR Cl ( ) channel activities in the apical membrane of trophectoderm cells . ^^^ |
|
| Interacting proteins: Q5T2W1 and P13569 |
Pubmed |
SVM Score :0.0 |
| Patch clamp recording of chloride channel activity revealed that there was a corresponding increase in chloride channel activity of Y1424A , I1427A CFTR , consistent with the elevated surface expression , and no change in CFTR channel properties . ^^^ |
|
| Interacting proteins: Q5T2W1 and P13569 |
Pubmed |
SVM Score :0.0 |
| Here we investigate the effects of the indazole compound lonidamine on CFTR channels expressed in mammalian cell lines using patch clamp recording . 2 . ^^^ |
|
| Interacting proteins: Q5T2W1 and P13569 |
Pubmed |
SVM Score :0.0 |
| In whole cell patch clamp experiments , the activation of endogenous AMPK either pharmacologically or by the overexpression of an AMPK activating non catalytic subunit mutant ( AMPK gamma 1 R70Q ) dramatically inhibited forskolin stimulated CFTR conductance in Calu 3 and CFTR expressing Chinese hamster ovary cells . ^^^ In contrast , the single channel open probability of CFTR was strongly reduced in cell attached patch clamp measurements of Calu 3 cells transfected with the AMPK activating mutant , an effect due primarily to a substantial prolongation of the mean closed time of the channel . ^^^ |
|
| Interacting proteins: Q5T2W1 and P13569 |
Pubmed |
SVM Score :0.0 |
| Whole cell clamp was used to analyze Cl ( ) currents in primary cultures of proximal and distal convoluted and cortical collecting tubules from wild type ( WT ) and cftr knockout ( KO ) mice . ^^^ |
|
| Interacting proteins: Q5T2W1 and P13569 |
Pubmed |
SVM Score :0.0 |
| Whole cell clamp was used to identify K ( + ) currents on the basis of pharmacological sensitivities in primary cultures of proximal ( PCT ) and distal convoluted tubule ( DCT ) and cortical collecting tubule ( CCT ) from wild type ( WT ) and CFTR knockout ( KO ) mice . ^^^ |
|
| Interacting proteins: Q5T2W1 and P13569 |
Pubmed |
SVM Score :0.0 |
| Single cell voltage clamp analysis showed characteristic CFTR currents after Delta F 508 CFTR activation . ^^^ |
|
| Interacting proteins: Q5T2W1 and P13569 |
Pubmed |
SVM Score :0.0 |
| The effect of intracellular cAMP and cystic fibrosis conductance regulator ( CFTR ) protein on the calcium activated chloride current ( ICaCl ) present in parotid acinar cells was studied using the patch clamp technique . ^^^ |
|
| Interacting proteins: Q5T2W1 and P13569 |
Pubmed |
SVM Score :0.0 |
| SPI 0211 effects on Cl currents were also measured by whole cell patch clamp using the human embryonic kidney ( HEK ) 293 cell line stably transfected with either recombinant human ClC 2 or recombinant human cystic fibrosis transmembrane regulator ( CFTR ) . ^^^ |
|
| Interacting proteins: Q5T2W1 and P13569 |
Pubmed |
SVM Score :0.0 |
| We have investigated whether changes in the extracellular anionic environment affects the activity of CFTR using the patch clamp technique . ^^^ |
|
| Interacting proteins: Q5T2W1 and P13569 |
Pubmed |
SVM Score :0.0 |
| We have used patch clamp recording to investigate the functional consequences of point mutations throughout the six transmembrane regions in the N terminal part of the CFTR protein ( TM 1 TM6 ) . ^^^ |
|
| Interacting proteins: Q5T2W1 and P13569 |
Pubmed |
SVM Score :0.0 |
| During a euglycemic clamp with infusion of insulin to physiologic postprandial levels , however , palmitate concentrations tended to be higher in CF patients : CF = 18 + / 3 ( 13 , 10 to 47 ) , control = 12 + / 1 ( 11 , 8 to 18 ) micromol / L , P = 0 . 08 . ^^^ |
|
| Interacting proteins: Q5T2W1 and P13569 |
Pubmed |
SVM Score :0.0 |
| CFTR and ORCC channel activity were measured with a whole cell configuration of the patch clamp technique . ^^^ |
|
| Interacting proteins: Q5T2W1 and P13569 |
Pubmed |
SVM Score :0.0 |
| To identify small molecules that rescue the gating defects of G551D and G1349D CFTR and understand better how these agents work , we used the patch clamp technique to study the effects on G551D and G1349D CFTR of phloxine B , pyrophosphate ( PP ( 1 ) ) , and 2 ' deoxy ATP ( 2 ' dATP ) , three agents that strongly enhance CFTR channel gating . ^^^ |
|
| Interacting proteins: Q5T2W1 and P13569 |
Pubmed |
SVM Score :0.0 |
| Here , we studied the contribution of the conserved residues G 551 and G 1349 to the pharmacological modulation of CFTR chloride channels by phloxine B using iodide efflux and whole cell patch clamp experiments performed on the following green fluorescent protein ( GFP ) tagged CFTR : wild type , delF 508 , G551D , G1349D , and G551D / G1349D double mutant . ^^^ |
|
| Interacting proteins: Q5T2W1 and P13569 |
Pubmed |
SVM Score :0.0 |
| ClC 3B as well as CFTR also binds EBP 50 ( ERM binding phosphoprotein 50 ) and PDZK 1 , which are concentrated at the plasma membrane . ^^^ |
|
| Interacting proteins: Q5T2W1 and P13569 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q5T2W1 and P13569 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q5T2W1 and P13569 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q5T2W1 and P13569 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q5T2W1 and P13569 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q5T2W1 and P13569 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q5T2W1 and P13569 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q5T2W1 and P13569 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q5T2W1 and P13569 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q5T2W1 and P13569 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q5T2W1 and P13569 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q5T2W1 and P13569 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q5T2W1 and P13569 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q5T2W1 and P13569 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q5T2W1 and P13569 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q5T2W1 and P13569 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q5T2W1 and P13569 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q5T2W1 and P13569 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q5T2W1 and P13569 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q5T2W1 and P13569 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q5T2W1 and P13569 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q5T2W1 and P13569 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q5T2W1 and P13569 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q5T2W1 and P13569 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q5T2W1 and P13569 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q5T2W1 and P13569 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q5T2W1 and P13569 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q5T2W1 and P13569 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q5T2W1 and P13569 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q5T2W1 and P13569 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q5T2W1 and P13569 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q5T2W1 and P13569 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q5T2W1 and P13569 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q5T2W1 and P13569 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q5T2W1 and P13569 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q5T2W1 and P13569 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q5T2W1 and P13569 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q5T2W1 and P13569 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q5T2W1 and P13569 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q5T2W1 and P13569 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q5T2W1 and P13569 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q5T2W1 and P13569 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q5T2W1 and P13569 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q5T2W1 and P13569 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q5T2W1 and P13569 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q5T2W1 and P13569 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q5T2W1 and P13569 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q5T2W1 and P13569 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q5T2W1 and P13569 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q5T2W1 and P13569 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q5T2W1 and P13569 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q5T2W1 and P13569 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q5T2W1 and P13569 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q5T2W1 and P13569 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q5T2W1 and P13569 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q5T2W1 and P13569 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q5T2W1 and P13569 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q5T2W1 and P13569 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q5T2W1 and P13569 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q5T2W1 and P13569 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q5T2W1 and P13569 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q5T2W1 and P13569 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q5T2W1 and P13569 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q5T2W1 and P13569 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q5T2W1 and P13569 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q5T2W1 and P13569 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q5T2W1 and P13569 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q5T2W1 and P13569 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q5T2W1 and P13569 |
Pubmed |
SVM Score :0.0 |
| NA |
|