| Endogenous , apical membrane CFTR in polarized human airway epithelial cells ( Calu 3 ) formed a complex with myosin 6 , the myosin 6 adaptor protein Disabled 2 ( Dab 2 ) , and clathrin . ^^^ The tail domain of myosin 6 , a dominant negative recombinant fragment , displaced endogenous myosin 6 from interacting with Dab 2 and CFTR and increased the expression of CFTR in the plasma membrane by reducing CFTR endocytosis . ^^^ Taken together , these data indicate that myosin 6 and Dab 2 facilitate CFTR endocytosis by a mechanism that requires actin filaments . . ^^^ |