| Interacting proteins: P15311 and P13569 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P15311 and P13569 |
Pubmed |
SVM Score :1.3981488 |
| Since ezrin has been shown previously to function as a protein kinase A anchoring protein , we suggest that one function served by the interaction of E3KARP with both ezrin and CFTR is to localize protein kinase A in the vicinity of the R domain of CFTR . 1.3981488^^^ E3KARP mediates the association of ezrin and protein kinase A with the cystic fibrosis transmembrane conductance regulator in airway cells . 0.81483011^^^ |
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| Interacting proteins: P15311 and P13569 |
Pubmed |
SVM Score :0.0 |
| In polarized epithelial cells , an adaptor molecule named EBP 50 provides a bridge between the amino terminal domain of ezrin and the cytoplasmic regions of plasma membrane proteins , including the cystic fibrosis transmembrane conductance regulator ( CFTR ) and the beta 2 adrenergic receptor . ^^^ |
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| Interacting proteins: P15311 and P13569 |
Pubmed |
SVM Score :0.0 |
| Since EBP 50 associates with ezrin , this interaction links the cystic fibrosis transmembrane conductance regulator ( CFTR ) to the cortical actin cytoskeleton . ^^^ |
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| Interacting proteins: P15311 and P13569 |
Pubmed |
SVM Score :0.0 |
| Taken together , these data demonstrate that PKA 2 is linked physically and functionally to CFTR by an AKAP interaction , and they suggest that ezrin serves as an AKAP for PKA mediated phosphorylation of CFTR . . ^^^ Protein kinase A associates with cystic fibrosis transmembrane conductance regulator via an interaction with ezrin . ^^^ |
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| Interacting proteins: P15311 and P13569 |
Pubmed |
SVM Score :0.0 |
| CFTR , NHE 3 , and ezrin were recognized by NHERF 2 but not NHERF 1 in glutathione S transferase pull down experiments . ^^^ Ezrin may function as a protein kinase A anchoring protein ( AKAP ) in this signaling complex , because blocking the binding of PKA to an AKAP by incubation with the S Ht 31 peptide inhibited the PKA dependent regulation of CFTR in the absence of NHE 3 . ^^^ We conclude that CFTR and NHE 3 reciprocally interact via a shared regulatory complex comprised of NHERF 2 , ezrin , and PKA . . ^^^ |
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| Interacting proteins: P15311 and P13569 |
Pubmed |
SVM Score :0.0 |
| The kinetics of the differentiation , polarity , and formation of the CFTR ZO 1 ezrin complex was analyzed by transmission , scanning , and immunofluorescence microscopy . ^^^ Our results show that after the initial step of dedifferentiation , characterized by a loss of ciliated cells and disappearance of epithelial subapical CFTR ezrin ZO 1 complex , the isolated cells formed 3 D spheroid structures within 24 hours . ^^^ After 35 days of 3 D culture , ZO 1 , CFTR , ezrin , and CD 59 were apically or subapically located , and well differentiated secretory and ciliated cells were identified . ^^^ |
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| Interacting proteins: P15311 and P13569 |
Pubmed |
SVM Score :0.0 |
| It has been proposed that the C terminal PDZ binding motif of CFTR is required for its apical membrane targeting and that PDZ domain interactions may tether CFTR to the actin cytoskeleton via soluble proteins including EBP50 / NHERF1 and ezrin . ^^^ |
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| Interacting proteins: P15311 and P13569 |
Pubmed |
SVM Score :0.0 |
| Here we show that binding of the FERM domain of ezrin to NHERF regulates the cooperative binding of NHERF to bring two cytoplasmic tails of CFTR into spatial proximity to each other . ^^^ We find that ezrin binding activates the second PDZ domain of NHERF to interact with the cytoplasmic tails of CFTR ( C CFTR ) , so as to form a specific 2 : 1 : 1 ( C CFTR ) ( 2 ) . ^^^ Without ezrin binding , the cytoplasmic tail of CFTR only interacts strongly with the first amino terminal PDZ domain to form a 1 : 1 C CFTR . ^^^ Immunoprecipitation and immunoblotting confirm the specific interactions of NHERF with the full length CFTR and with ezrin in vivo . ^^^ Ezrin controls the macromolecular complexes formed between an adapter protein Na+ / H+ exchanger regulatory factor and the cystic fibrosis transmembrane conductance regulator . ^^^ |
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| Interacting proteins: P15311 and P13569 |
Pubmed |
SVM Score :0.0 |
| It has been shown that when CFTR and NHE 3 are co expressed on the apical membrane of the A 6 NHE3 cell monolayers , the two transporters interact via a shared regulatory complex composed of NHERF 2 , ezrin , and PKA . ^^^ |
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