| Interacting proteins: P06753 and P13569 |
Pubmed |
SVM Score :0.0 |
| In contrast , TM 3 and TM 4 of another polytopic membrane protein , cystic fibrosis transmembrane conductance regulator ( CFTR ) , have a similar level of de novo Nin Cout membrane insertion activity and TM 4 of CFTR functions only as a stop transfer sequence in the presence of TM 3 . ^^^ |
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| Interacting proteins: P06753 and P13569 |
Pubmed |
SVM Score :0.0 |
| To understand how other TM segments are ensured to insert into membranes correctly , we investigated the topogenesis of TM 3 and TM 4 of CFTR in a cell free expression system . ^^^ We found that the correct membrane insertion of TM 3 and TM 4 of CFTR was ensured by their flanking amino acid sequences and controlled by the correct membrane insertion of their preceding TM 1 and TM 2 . ^^^ |
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| Interacting proteins: P06753 and P13569 |
Pubmed |
SVM Score :0.0 |
| Here we have undertaken an investigation of the presence and strength of H bond interactions within a series of helix loop helix ( `` hairpin ' ' ) constructs derived from TM helices 3 and 4 ( italic ) of the cystic fibrosis transmembrane conductance regulator ( CFTR ) ( prototypic sequence G ( 194 ) LALAHFVWIAPLQ ( 207 ) VALLMGLIWELLQASAFAGLGFLIV ( 232 ) LALFQ ( 237 ) AGLG ( 241 ) ) in which wild type Q 207 in TM 3 forms an interhelical H bond with CF phenotypic mutant V232D in TM 4 [ Therien , A . ^^^ |
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| Interacting proteins: P06753 and P13569 |
Pubmed |
SVM Score :0.0 |
| We compared the effects of mutations in transmembrane segments ( TMs ) TM 1 , TM 5 , and TM 6 on the conduction and activation properties of the cystic fibrosis transmembrane conductance regulator ( CFTR ) to determine which functional property was most sensitive to mutations and , thereby , to develop a criterion for measuring the importance of a particular residue or TM for anion conduction or activation . ^^^ Mutations in TM 5 and TM 6 also dramatically reduced the sensitivity of CFTR to activation by 3 isobutyl 1 methyl xanthine ( IBMX ) , as expected if these TMs are intimately involved in the physical process that opens and closes the channel . . ^^^ |
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| Interacting proteins: P06753 and P13569 |
Pubmed |
SVM Score :0.0 |
| Cross linking between TM 4 or TM 5 and TM 12 was also detected when residues ( 483 ) TIAENIRYG ( 491 ) in P gp were replaced with residues ( 501 ) TIKENIIFG ( 509 ) from CFTR ( P gp / CFTR ) . ^^^ Deleting Phe ( 508 ) in the P gp / CFTR chimera , however , caused defective processing of the mutant protein and no detectable cross linking between TM 4 or TM 5 and TM 12 . ^^^ |
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| Interacting proteins: P06753 and P13569 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06753 and P13569 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06753 and P13569 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06753 and P13569 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06753 and P13569 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06753 and P13569 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06753 and P13569 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06753 and P13569 |
Pubmed |
SVM Score :0.0 |
| NA |
|