| Interacting proteins: O14745 and P13569 |
Pubmed |
SVM Score :0.52087431 |
| Here we report that CFTR interacts with the recently discovered protein , EBP 50 ( ERM binding phosphoprotein 50 ) . 0.52087431^^^ Through formation of a complex , the interaction between CFTR and EBP 50 may influence the stability and / or regulation of CFTR Cl channel function in the cell membrane and provides a potential mechanism through which CFTR can affect the activity of other apical membrane proteins . . 0.51109949^^^ |
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| Interacting proteins: O14745 and P13569 |
Pubmed |
SVM Score :0.92602746 |
| Our goal was to identify the key amino acid ( s ) in the PDZ interacting domain of CFTR with regard to its apical polarization , interaction with EBP 50 , and ability to mediate transepithelial chloride secretion . 0.92602746^^^ |
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| Interacting proteins: O14745 and P13569 |
Pubmed |
SVM Score :0.52972336 |
| These data suggest that beta ( 2 ) AR activation regulates the association of CFTR with EBP 50 in polarized HAEC . . 0.52972336^^^ |
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| Interacting proteins: O14745 and P13569 |
Pubmed |
SVM Score :0.7982709 |
| We also demonstrate that CFTR binds to ezrin radixin moesin binding phosphoprotein 50 ( EBP 50 ) , an apical membrane PDZ domain containing protein . 0.7982709^^^ |
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| Interacting proteins: O14745 and P13569 |
Pubmed |
SVM Score :0.59684756 |
| By using human airway epithelial cells , we show that CFTR associates with Na ( + ) / H ( + ) exchanger ( NHE ) type 3 kinase A regulatory protein ( E3KARP ) , an EBP50 / NHE regulatory factor ( NHERF ) related PDZ domain protein . 0.59684756^^^ |
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| Interacting proteins: O14745 and P13569 |
Pubmed |
SVM Score :0.62155859 |
| The PDZ 1 domain of the Na ( + ) / H ( + ) exchanger regulatory factor ( NHERF ) binds with nanomolar affinity to the carboxyl terminal sequence QDTRL of the cystic fibrosis transmembrane conductance regulator ( CFTR ) and plays a central role in the cellular localization and physiological regulation of this chloride channel . 0.62155859^^^ |
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| Interacting proteins: O14745 and P13569 |
Pubmed |
SVM Score :1.4544824 |
| In the present work , we describe experiments to define the interactions between CFTR and NHE 3 with the regulatory , scaffolding protein , NHERF that organize their PKA dependent regulation in a renal epithelial cell line that expresses endogenous CFTR . 1.4544824^^^ |
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| Interacting proteins: O14745 and P13569 |
Pubmed |
SVM Score :0.53604376 |
| Complex formation between CFTR and NHERF was disrupted by C terminal deletions , C terminal epitope tag attachments , or overexpression of a dominant negative NHERF mutant . 0.53604376^^^ |
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| Interacting proteins: O14745 and P13569 |
Pubmed |
SVM Score :0.71663564 |
| Immunoprecipitation and immunoblotting confirm the specific interactions of NHERF with the full length CFTR and with ezrin in vivo . 0.71663564^^^ |
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| Interacting proteins: O14745 and P13569 |
Pubmed |
SVM Score :0.0 |
| ClC 3B , a novel ClC 3 splicing variant that interacts with EBP 50 and facilitates expression of CFTR regulated ORCC . ^^^ When cystic fibrosis transmembrane conductance regulator ( CFTR ) was cotransfected with ClC 3B and EBP 50 , ClC 3B dependent ORCCs were activated via the protein kinase A dependent pathway . ^^^ |
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| Interacting proteins: O14745 and P13569 |
Pubmed |
SVM Score :0.0 |
| In polarized epithelial cells , an adaptor molecule named EBP 50 provides a bridge between the amino terminal domain of ezrin and the cytoplasmic regions of plasma membrane proteins , including the cystic fibrosis transmembrane conductance regulator ( CFTR ) and the beta 2 adrenergic receptor . ^^^ |
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| Interacting proteins: O14745 and P13569 |
Pubmed |
SVM Score :0.0 |
| Since EBP 50 associates with ezrin , this interaction links the cystic fibrosis transmembrane conductance regulator ( CFTR ) to the cortical actin cytoskeleton . ^^^ EBP 50 has two PDZ domains , and CFTR binds with high affinity to the first PDZ domain . ^^^ We recently showed that the COOH terminus of the cystic fibrosis transmembrane conductance regulator associates with the submembranous scaffolding protein EBP 50 ( ERM binding phosphoprotein 50 kD ; also called Na ( + ) / H ( + ) exchanger regulatory factor ) . ^^^ |
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| Interacting proteins: O14745 and P13569 |
Pubmed |
SVM Score :0.0 |
| However , this higher order complex is predominantly CFTR dimers , and the PDZ interacting partners ( Na ( + ) / H ( + ) exchanger regulatory factor 1 ( NHERF 1 ) and NHERF 2 ) constitute approximately 2 % of this complex . ^^^ |
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| Interacting proteins: O14745 and P13569 |
Pubmed |
SVM Score :0.0 |
| Precipitation of NBC 3 or CFTR from transfected HEK 293 cells and from the pancreas and submandibular gland also coimmunoprecipitated EBP 50 . ^^^ Glutathione S transferase EBP 50 pulled down CFTR and hNBC 3 from cell lysates when expressed individually and as a complex when expressed together . ^^^ |
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| Interacting proteins: O14745 and P13569 |
Pubmed |
SVM Score :0.0 |
| ClC 3B as well as CFTR also binds EBP 50 ( ERM binding phosphoprotein 50 ) and PDZK 1 , which are concentrated at the plasma membrane . ^^^ |
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| Interacting proteins: O14745 and P13569 |
Pubmed |
SVM Score :0.0 |
| It has been demonstrated previously that both the cystic fibrosis transmembrane conductance regulator ( CFTR ) and beta ( 2 ) adrenergic receptor ( beta ( 2 ) AR ) can bind ezrinradixinmoesin binding phosphoprotein 50 ( EBP 50 , also referred to as NHERF ) through their PDZ motifs . ^^^ Biochemical studies demonstrate the existence of a macromolecular complex involving CFTR EBP 50 beta ( 2 ) AR through PDZ based interactions . ^^^ |
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| Interacting proteins: O14745 and P13569 |
Pubmed |
SVM Score :0.0 |
| Expression of constructs encoding wild type or dominant negative forms of the PDZ binding protein EBP 50 suggests that EBP 50 may be involved in CFTR dependent RANTES expression . ^^^ |
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| Interacting proteins: O14745 and P13569 |
Pubmed |
SVM Score :0.0 |
| EBP 50 ( NHERF 1 ) , a PDZ domain containing protein that interacts with the C terminus of CFTR , diffused freely in the cytoplasm with a diffusion coefficient of 0 . 9 + / 0 . 1 10 10 ( 7 ) cm2 / s . ^^^ |
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| Interacting proteins: O14745 and P13569 |
Pubmed |
SVM Score :0.0 |
| The association of the cystic fibrosis transmembrane regulator ( CFTR ) with two PDZ containing molecular scaffolds ( CAL and EBP 50 ) plays an important role in CFTR trafficking and membrane maintenance . ^^^ The R ( ) ( 1 ) and D ( ) ( 3 ) side chains make a number of contacts with the PDZ domain ; many of these interactions differ from those in the CFTR EBP 50 complex , suggesting sites that can be targeted in the development of PDZ selective inhibitors that may help modulate CFTR function . . ^^^ |
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| Interacting proteins: O14745 and P13569 |
Pubmed |
SVM Score :0.0 |
| Using overlay assay , immunoprecipitation , pulldown and binding assays , we show that PKC epsilon does not bind to CFTR , but does bind to a receptor for activated C kinase ( RACK 1 ) , a 37 kDa scaffold protein , and that RACK 1 binds to Na ( + ) / H ( + ) exchange regulatory factor ( NHERF 1 ) , a binding partner of CFTR . ^^^ The results indicate the RACK 1 binds PKC epsilon and NHERF 1 , thus serving as a scaffold protein to anchor the enzyme in proximity to CFTR . . ^^^ |
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| Interacting proteins: O14745 and P13569 |
Pubmed |
SVM Score :0.0 |
| Both NHERF proteins contain two PDZ domains ; recombinant protein protein binding assays and yeast two hybrid studies revealed that ROMK preferentially associates with the second PDZ domain of NHERF 1 and with the first PDZ domain of NHERF 2 , precisely opposite of what has been reported for CFTR . ^^^ |
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| Interacting proteins: O14745 and P13569 |
Pubmed |
SVM Score :0.0 |
| In past studies , we demonstrated regulation of CFTR Cl channel function by protein kinase C ( PKC ) epsilon through the binding of PKC epsilon to RACK 1 ( a receptor for activated C kinase ) and of RACK 1 to human Na ( + ) / H ( + ) exchanger regulatory factor ( NHERF 1 ) . ^^^ An internal 11 amino acid motif embedding the GYGF carboxylate binding loop of PDZ 1 binds to RACK 1 , inhibits binding of recombinant NHERF 1 and RACK 1 , pulls down endogenous RACK 1 from Calu 3 cell lysate , and blocks coimmunoprecipitation of endogenous RACK 1 with endogenous NHERF 1 but does not affect cAMP dependent activation of CFTR . ^^^ |
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| Interacting proteins: O14745 and P13569 |
Pubmed |
SVM Score :0.0 |
| The Na ( + ) / H ( + ) exchanger regulatory factor isoform 1 ( NHERF 1 ) was the first identified CFTR binding protein . ^^^ Here we further clarify the role of NHERF 1 in the regulation of CFTR activity in two human bronchial epithelial cell lines : the normal , 16HBE14o , and the homozygous DeltaF 508 CFTR , CFBE41o . ^^^ Transfection of 16HBE14o monolayers with vectors encoding for wild type ( wt ) NHERF 1 increased both apical CFTR expression and apical protein kinase A ( PKA ) dependent CFTR mediated chloride efflux , whereas transfection with NHERF 1 mutated in the binding groove of the PDZ domains or truncated for the ERM domain inhibited both the apical CFTR expression and the CFTR dependent chloride efflux . ^^^ These data led us to hypothesize an important role for NHERF 1 in regulating CFTR localization and stability on the apical membrane of 16HBE14o cell monolayers . ^^^ Importantly , wt NHERF 1 overexpression in confluent DeltaF 508 CFBE41o and DeltaF 508 CFT1 C 2 cell monolayers induced both a significant redistribution of CFTR from the cytoplasm to the apical membrane and a PKA dependent activation of CFTR dependent chloride secretion . . ^^^ |
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| Interacting proteins: O14745 and P13569 |
Pubmed |
SVM Score :0.0 |
| Regulation of the CFTR Cl channel function involves a protein complex of activated protein kinase Cepsilon ( PKCepsilon ) bound to RACK 1 , a receptor for activated C kinase , and RACK 1 bound to the human Na ( + ) / H ( + ) exchanger regulatory factor ( NHERF 1 ) in human airway epithelial cells . ^^^ |
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| Interacting proteins: O14745 and P13569 |
Pubmed |
SVM Score :0.0 |
| Peptide binding consensus of the NHE RF PDZ 1 domain matches the C terminal sequence of cystic fibrosis transmembrane conductance regulator ( CFTR ) . ^^^ Because PDZ domains usually bind to specific short linear C terminal sequences , we have carried out affinity selection of random peptides for specific sequences that interact with the NHE RF PDZ domains and found that NHE RF PDZ 1 is capable of binding to the CFTR C terminus . ^^^ The specific and tight association suggests a potential regulatory role of NHE RF in cystic fibrosis transmembrane conductance regulator ( CFTR ) function . . ^^^ |
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| Interacting proteins: O14745 and P13569 |
Pubmed |
SVM Score :0.0 |
| NHERF also binds to the tail of the cystic fibrosis transmembrane conductance regulator , which ends in D T R L . ^^^ |
|
| Interacting proteins: O14745 and P13569 |
Pubmed |
SVM Score :0.0 |
| Association with NHERF may also regulate the cystic fibrosis transmembrane conductance regulator and the sodium bicarbonate transporter . ^^^ |
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| Interacting proteins: O14745 and P13569 |
Pubmed |
SVM Score :0.0 |
| The Na ( + ) / H ( + ) exchanger regulatory factor ( NHERF ) binds to the cytoplasmic tail of CFTR through either of its two PDZ ( PDZ 1 and PDZ 2 ) domains . ^^^ A recombinant fragment of NHERF ( PDZ 1 2 ) containing the two PDZ domains increases the open probability ( P ( o ) ) of single CFTR channels in excised membrane patches from a lung submucosal gland cell line . ^^^ Our results support a molecular model in which bivalent NHERF PDZ domains regulate channel gating by crosslinking the C terminal tails in a single dimeric CFTR channel , and the magnitude of this regulation is coupled to the stoichiometry of these interactions . . ^^^ |
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| Interacting proteins: O14745 and P13569 |
Pubmed |
SVM Score :0.0 |
| The human NHERF PDZ 1 domain , which spans residues 11 99 , interacts specifically with carboxy terminal residues of the beta 2 adrenergic receptor and the cystic fibrosis transmembrane conductance regulator . ^^^ |
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| Interacting proteins: O14745 and P13569 |
Pubmed |
SVM Score :0.0 |
| The NHERF PDZ 1 domain interacts specifically with the motifs DSLL , DSFL , and DTRL present at the carboxyl termini of the beta ( 2 ) adrenergic receptor ( beta ( 2 ) AR ) , the platelet derived growth factor receptor ( PDGFR ) , and the cystic fibrosis transmembrane conductance regulator ( CFTR ) , respectively , and plays a central role in the physiological regulation of these proteins . ^^^ This structure reveals the molecular mechanism of carboxyl terminal leucine recognition by class 1 PDZ domains , and provides insights into the specificity of NHERF interaction with the carboxyl termini of several membrane receptors and ion channels , including the beta ( 2 ) AR , PDGFR , and CFTR . . ^^^ |
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| Interacting proteins: O14745 and P13569 |
Pubmed |
SVM Score :0.0 |
| Although identified as a regulator of NHE 3 , recent evidence points to a broadening role for NHERF in the function , localization and / or turnover of G protein coupled receptors , platelet derived growth factor receptor and ion transporters such as CFTR , Na / Pi cotransporter , Na / HCO3 cotransporter and Trp ( calcium ) channels . ^^^ NHERF overexpression in human breast cancers and mutations in NHERF targets , such as CFTR and merlin , the product of Neurofibromatosis NF 2 tumor suppressor gene , that impair NHERF binding suggest that aberrant NHERF function contributes to human disease . . ^^^ |
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| Interacting proteins: O14745 and P13569 |
Pubmed |
SVM Score :0.0 |
| The Na ( + ) / H ( + ) exchanger regulatory factor , NHE RF , a subplasma membrane PDZ domain protein , restores cell surface expression of CFTR and chloride currents . ^^^ In addition , NHE RF inhibits the binding of CAL to CFTR . ^^^ CAL favors retention of CFTR within the cell , whereas NHE RF favors surface expression by competing with CAL for the binding of CFTR . ^^^ |
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| Interacting proteins: O14745 and P13569 |
Pubmed |
SVM Score :0.0 |
| The Na+ / H+ exchanger regulatory factor ( NHERF ) binds to CFTR and increases its open probability ( Po ) . ^^^ Protein kinase C disrupts the stimulatory effect of NHERF on CFTR channel Po . ^^^ Furthermore , a mutation in PDZ 2 that mimics phosphorylation decreases CFTR binding and disrupts the ability of NHERF PDZ 1 2 to stimulate CFTR channel Po . ^^^ Our results identify a role for PKC and suggest that phosphorylation of NHERF PDZ 2 domain may be an important mechanism for regulating CFTR channel activity . . ^^^ |
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| Interacting proteins: O14745 and P13569 |
Pubmed |
SVM Score :0.0 |
| In the present study we demonstrate that the Na+ / H+ exchanger regulator factor ( NHERF ) controls expression of CFTR in mouse collecting duct cells . ^^^ Inhibition of NHERF largely attenuates CFTR expression , which is paralleled by enhanced Ca ( 2+ ) dependent Cl secretion and augmented Na+ absorption by the ENaC . ^^^ |
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| Interacting proteins: O14745 and P13569 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O14745 and P13569 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O14745 and P13569 |
Pubmed |
SVM Score :0.0 |
| NA |
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