| Interacting proteins: P13533 and Q01449 |
Pubmed |
SVM Score :0.0 |
| We developed novel , fluorescent based , myocardial , cellular transplantation systems in order to study these questions in murine embryos and report the irreversible nature of chamber specification with respect to the downregulation of atrial myosin light chain 2 ( MLC 2a ) and alpha myosin heavy chain ( alpha MHC ) . ^^^ Additionally , wild type ventricular muscle cells grafted into the wild type background appropriately downregulate MLC 2a and alpha MHC . ^^^ |
|
| Interacting proteins: P13533 and Q01449 |
Pubmed |
SVM Score :0.0 |
| We now demonstrate that three myosin genes which become transcriptionally restricted to the atria between embryonic day ( E ) 12 . 5 and birth , alpha myosin heavy chain ( MHC ) , myosin light chain ( MLC ) 1A and MLC2A , are coordinately downregulated in the compact myocardium of the left ventricle before that of the right ventricle . alpha MHC protein also accumulates in the right , but not left , compact ventricular myocardium during this period , suggesting that this transient regionalization contributes to fktal heart function . dHAND and eHAND , basic helix loop helix transcription factors known to be expressed in the right and left ventricles respectively at E 10 . 5 , remain regionalized between E12 . 5 and E14 . 5 . ^^^ Downregulation of alpha MHC , MLC1A , and MLC2A in iv / iv embryos , which have defective left / right patterning , initiates in the morphological left ( systemic ) ventricle regardless of its anatomical position on the right or left hand side of the heart . ^^^ |
|
| Interacting proteins: P13533 and Q01449 |
Pubmed |
SVM Score :0.0 |
| Semiquantitative reverse transcription polymerase chain reaction analyses demonstrated expression of marker genes typical for endoderm , mesoderm , cardiac mesoderm , and CMs , including brachyury , goosecoid , Tbx 5 , Tbx 20 , Mesp 1 , Nkx2 . 5 , GATA 4 , FOG 2 , Mlc2a , MLC2v , ANF , and alpha MHC in rESC derived CMs . ^^^ |
|
| Interacting proteins: P13533 and Q01449 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13533 and Q01449 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q01449 and P13533 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q01449 and P13533 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q01449 and P13533 |
Pubmed |
SVM Score :0.0 |
| We developed novel , fluorescent based , myocardial , cellular transplantation systems in order to study these questions in murine embryos and report the irreversible nature of chamber specification with respect to the downregulation of atrial myosin light chain 2 ( MLC 2a ) and alpha myosin heavy chain ( alpha MHC ) . ^^^ Additionally , wild type ventricular muscle cells grafted into the wild type background appropriately downregulate MLC 2a and alpha MHC . ^^^ |
|
| Interacting proteins: Q01449 and P13533 |
Pubmed |
SVM Score :0.0 |
| We now demonstrate that three myosin genes which become transcriptionally restricted to the atria between embryonic day ( E ) 12 . 5 and birth , alpha myosin heavy chain ( MHC ) , myosin light chain ( MLC ) 1A and MLC2A , are coordinately downregulated in the compact myocardium of the left ventricle before that of the right ventricle . alpha MHC protein also accumulates in the right , but not left , compact ventricular myocardium during this period , suggesting that this transient regionalization contributes to fktal heart function . dHAND and eHAND , basic helix loop helix transcription factors known to be expressed in the right and left ventricles respectively at E 10 . 5 , remain regionalized between E12 . 5 and E14 . 5 . ^^^ Downregulation of alpha MHC , MLC1A , and MLC2A in iv / iv embryos , which have defective left / right patterning , initiates in the morphological left ( systemic ) ventricle regardless of its anatomical position on the right or left hand side of the heart . ^^^ |
|
| Interacting proteins: Q01449 and P13533 |
Pubmed |
SVM Score :0.0 |
| Semiquantitative reverse transcription polymerase chain reaction analyses demonstrated expression of marker genes typical for endoderm , mesoderm , cardiac mesoderm , and CMs , including brachyury , goosecoid , Tbx 5 , Tbx 20 , Mesp 1 , Nkx2 . 5 , GATA 4 , FOG 2 , Mlc2a , MLC2v , ANF , and alpha MHC in rESC derived CMs . ^^^ |
|