| Interacting proteins: Q13426 and P12956 |
Pubmed |
SVM Score :0.0 |
| Genes essential for the end joining reaction include : XRCC 4 encoding a protein of unknown enzymatic function ; XRCC 5 and XRCC 6 encoding 86 and 70 kDa subunits of the Ku autoantigen , a DNA end binding protein that is also the regulatory subunit of DNA dependent protein kinase ( DNA PK ) ; and XRCC 7 encoding the catalytic subunit ( DNA PKcs ) of DNA PK . ^^^ |
|
| Interacting proteins: Q13426 and P12956 |
Pubmed |
SVM Score :0.0 |
| From the analysis of defective rodent mutants , four proteins ( Ku 70 , Ku 80 , DNA PKcs and Xrcc 4 ) that function in this process in mammalian cells have been identified . ^^^ |
|
| Interacting proteins: Q13426 and P12956 |
Pubmed |
SVM Score :0.0 |
| These factors include components of DNA dependent protein kinase ( DNA PK ) , namely DNA PKcs and the Ku heterodimer , Ku 70 Ku80 , and XRCC 4 . ^^^ |
|
| Interacting proteins: Q13426 and P12956 |
Pubmed |
SVM Score :0.0 |
| Aside from DNA PK , XRCC 4 and DNA ligase 4 , other proteins which play a role ( s ) in this repair pathway remain unknown ; DNA PK contains a catalytic subunit ( DNA PKcs ) and a DNA binding subunit ( Ku 70 and Ku 80 ) . ^^^ |
|
| Interacting proteins: Q13426 and P12956 |
Pubmed |
SVM Score :0.0 |
| DNA double strand breaks formed during the assembly of antigen receptors or after exposure to ionizing radiation are repaired by proteins important for nonhomologous end joining that include Ku 86 , Ku 70 , DNA PK ( CS ) , Xrcc 4 , and DNA ligase 4 . ^^^ |
|
| Interacting proteins: Q13426 and P12956 |
Pubmed |
SVM Score :0.0 |
| Any defect in one of the known components of the DNA repair / V ( D ) J recombination machinery ( Ku 70 , Ku 80 , DNA PKcs , XRCC 4 and DNA ligase 4 ) leads to abortion of the 5 ( D ) J rearrangement process , early block in both T and B cell maturation , and ultimately to severe combined immune deficiency ( SCID ) in several animal models . ^^^ |
|
| Interacting proteins: Q13426 and P12956 |
Pubmed |
SVM Score :0.0 |
| Using Western blots we measured , in non irradiated cells , the basal expression levels of ATM , Rad 1 and Hus 1 , involved in the control of cellular DNA damage checkpoints , together with DNA PKcs , Ku 70 , Ku 80 ; XRCC 4 , ligaseIV and Rad 51 , involved in radiation induced DSB repair . ^^^ |
|
| Interacting proteins: Q13426 and P12956 |
Pubmed |
SVM Score :0.0 |
| Mammalian nonhomologous DNA end joining employs Ku 70 , Ku 80 , DNA dependent protein kinase catalytic subunit ( DNA PKcs ) , XRCC 4 , and DNA ligase 4 ( Lig 4 ) . ^^^ |
|
| Interacting proteins: Q13426 and P12956 |
Pubmed |
SVM Score :0.0 |
| Protein expression levels of the DNA dependent protein kinase ( DNA PK ) / Ku dependent nonhomologous DNA end joining proteins Xrcc 4 , DNA ligase 4 , Ku 70 , and Ku 86 in FA and normal extracts were indistinguishable , as were DNA dependent protein kinase and DNA end binding activities . ^^^ |
|
| Interacting proteins: Q13426 and P12956 |
Pubmed |
SVM Score :0.0 |
| DNA PKcs , Ku 70 , Ku 85 , Xrcc 4 , and Nbs 1 play a critical role in DNA DSB repair . ^^^ METHODS : We immunohistochemically investigated the expression of DNA PKcs , Ku 70 , Ku 85 , Xrcc 4 , and Nbs 1 in 134 specimens from various normal and tumor tissues with different radiosensitivity . ^^^ RESULTS AND CONCLUSION : Immunopositivity to Ku 70 , Ku 85 , DNA PKcs , Xrcc 4 , and Nbs 1 was found in all tumor tissues examined . ^^^ The staining for Ku 70 , Ku 85 , and DNA PKcs was nuclear ; but , for Xrcc 4 and Nbs 1 , it was nuclear and cytoplasmic . ^^^ |
|
| Interacting proteins: Q13426 and P12956 |
Pubmed |
SVM Score :0.0 |
| DNA double strand break ( DSB ) repair by non homologous end joining ( NHEJ ) in mammalian cells requires the Ku 70 Ku80 heterodimer , the DNA PK catalytic subunit DNA PKcs , as well as DNA ligase 4 and Xrcc 4 . ^^^ |
|
| Interacting proteins: Q13426 and P12956 |
Pubmed |
SVM Score :0.0 |
| Ku 86 together with Ku 70 , DNA PKcs , XRCC 4 and DNA ligase 4 forms a complex involved in repairing DNA double strand breaks ( DSB ) in mammals . ^^^ |
|
| Interacting proteins: Q13426 and P12956 |
Pubmed |
SVM Score :0.0 |
| We find that the HDR frequency is enhanced in Ku 70 ( / ) , XRCC 4 ( / ) , and DNA PKcs ( / ) cells , with the increase being particularly striking in Ku 70 ( / ) cells . ^^^ |
|
| Interacting proteins: Q13426 and P12956 |
Pubmed |
SVM Score :0.0 |
| NHEJ can be catalyzed by mammalian whole cell extract in a reaction dependent upon DNA ligase 4 , Xrcc 4 , Ku 70 , Ku 80 , and DNA PKcs . ^^^ The Brca 1 dependent NHEJ activity predominates at the lower concentrations of Mg2+ ( 0 . 5 mM ) ; elevated Mg2+ or Mn2+ concentrations ( 10 mM ) dramatically increase overall end joining activity and abrogates the requirement for Brca 1 , Xrcc 4 , and Ku 70 . ^^^ |
|
| Interacting proteins: Q13426 and P12956 |
Pubmed |
SVM Score :0.0 |
| Detailed examination indicates that the patient is defective neither in the known factors involved in NHEJ in mammals ( Ku 70 , Ku 80 , DNA dependent protein kinase catalytic subunit , Xrcc 4 , DNA ligase 4 , or Artemis ) nor in the Mre11 / Rad50 / Nbs1 complex , whose homologue in Saccharomyces cerevisiae functions in NHEJ . ^^^ |
|
| Interacting proteins: Q13426 and P12956 |
Pubmed |
SVM Score :0.0 |
| We did not observe any abnormalities in expression level or migration pattern of the following NHEJ proteins in radiosensitive cancer cases : Ku 70 , Ku 80 , XRCC 4 , DNA Ligase 4 . ^^^ |
|
| Interacting proteins: Q13426 and P12956 |
Pubmed |
SVM Score :0.0 |
| The non homologous end joining ( NHEJ ) pathway of DNA recombination is important for genomic stability in animal cells , since the absence of Ku 70 , Ku 80 , Lig 4 or Xrcc 4 results in non reciprocal translocation and chromosome fragmentation . ^^^ |
|
| Interacting proteins: Q13426 and P12956 |
Pubmed |
SVM Score :0.0 |
| Because single nucleotide polymorphisms ( SNPs ) are the most subtle genetic variation in the genome , to examine these hypotheses , we have genotyped 30 SNPs in all five NHEJ genes ( Ku 70 , Ku 80 , DNA PKcs , Ligase 4 , and XRCC 4 ) in 254 primary breast cancer patients and 379 healthy controls . ^^^ |
|
| Interacting proteins: Q13426 and P12956 |
Pubmed |
SVM Score :0.0 |
| The main components of the NHEJ system in eukaryotes are the catalytic subunit of DNA protein kinase ( DNA PK ( cs ) ) , which is recruited by DNA Ku protein , a heterodimer of Ku 70 and Ku 80 , as well as XRCC 4 protein and DNA ligase 4 . ^^^ |
|
| Interacting proteins: Q13426 and P12956 |
Pubmed |
SVM Score :0.0 |
| Ku 70 , Ku 80 , DNA PKcs ( the catalytic subunit of DNA dependent protein kinase ) , Xrcc 4 and Artemis all participate in DSB repair through nonhomologous end joining ( NHEJ ) . ^^^ |
|
| Interacting proteins: Q13426 and P12956 |
Pubmed |
SVM Score :0.0 |
| Western blot analysis indicated that the expression of NHEJ pathway components Ku 70 , XRCC 4 , and DNA ligase 4 was downregulated in NSCLC cells A 549 with Ad mda 7 treatment . ^^^ |
|
| Interacting proteins: Q13426 and P12956 |
Pubmed |
SVM Score :0.0 |
| Intra and inter pathway interactions were quantified by co localisation , FRET imaging and by co immunoprecipitation ( Co IP ) of XRCC 4 , DNA PK and Ku 70 as representatives of NHEJ , Rad 51 and Rad 52 for HRR and gamma H2AX , Mre 11 and Rad 50 as representatives of both pathways . ^^^ |
|
| Interacting proteins: Q13426 and P12956 |
Pubmed |
SVM Score :0.0 |
| This band was also completely supershifted by the addition of antibodies to ATM , Ku 70 , DNA ligase 3 , Rpa 32 , Rpa 14 , DNA ligase 4 , XRCC 4 , WRN , BLM , RAD 51 and p 53 . ^^^ The density of one of the DNA EBCs predicted the radiosensitization caused by BRCA haploinsufficiency , and this band contains Ku 70 , ATM , DNA ligase 3 , Rpa 32 , Rpa 14 , DNA ligase 4 , XRCC 4 , WRN , BLM , RAD 51 and p 53 but not BRCA 1 or 2 . ^^^ |
|
| Interacting proteins: Q13426 and P12956 |
Pubmed |
SVM Score :0.0 |
| Junctions were formed by the non random deletion of terminal nucleotides and showed a preference for annealing at a microhomology of 8 nt buried within the DNA substrate ; this process was not dependent on functional Ku 70 , DNA PK or XRCC 4 . ^^^ |
|
| Interacting proteins: Q13426 and P12956 |
Pubmed |
SVM Score :0.0 |
| The budding yeast Saccharomyces cerevisiae repairs DSB primarily by homologous recombination ( HR ) , despite the presence of the KU 70 , KU 80 , DNA ligase 4 and XRCC 4 homologues , essential factors of the mammalian non homologous end joining ( NHEJ ) machinery . ^^^ |
|
| Interacting proteins: Q13426 and P12956 |
Pubmed |
SVM Score :0.0 |
| The core components of mammalian NHEJ are the catalytic subunit of DNA protein kinase ( DNA PK ( cs ) ) , Ku subunits Ku 70 and Ku 80 , Artemis , XRCC 4 and DNA ligase 4 . ^^^ |
|
| Interacting proteins: Q13426 and P12956 |
Pubmed |
SVM Score :0.0 |
| Twelve loci of selected DNA repair genes were genotyped by matrix assisted laser desorption / ionization time of flight mass spectrometry ( XRCC 2 Arg188His , XRCC 4 921G > T , XRCC 6 1796G > T , LIG 4 1977T / C , RAD 51 135G > C , 172G > T , RAD 52 2259C > T , LIG 1 551A > C , ERCC 1 8092A > C , 354C > T , hMLH 1 93G > A , and Ile219Val ) . ^^^ |
|
| Interacting proteins: Q13426 and P12956 |
Pubmed |
SVM Score :0.0 |
| LOH was recurrently observed for 8 of the 9 studied genes ( KU 70 , KU 80 , XRCC 4 , LIG 4 , Artemis , MRE 11 , RAD 50 , NBS 1 ) but not for DNA PKcs . ^^^ |
|
| Interacting proteins: Q13426 and P12956 |
Pubmed |
SVM Score :0.0 |
| Ku 70 , DNA PKcs , DNA Ligase 4 , Xrcc 4 ) were observed . ^^^ |
|
| Interacting proteins: Q13426 and P12956 |
Pubmed |
SVM Score :0.0 |
| As expected , 5 ( D ) J recombination was substantially impaired in cells deficient for the NHEJ components Ku 70 , XRCC 4 , and DNA PKcs . ^^^ |
|
| Interacting proteins: Q13426 and P12956 |
Pubmed |
SVM Score :0.0 |
| DNA double strand breaks ( dsb ) during 5 ( D ) J recombination of T and B lymphocyte receptor genes are resolved by the non homologous DNA end joining pathway ( NHEJ ) including at least six factors : Ku 70 , Ku 80 , DNA PK ( cs ) , Artemis , Xrcc 4 , and DNA ligase 4 ( Lig 4 ) . ^^^ |
|
| Interacting proteins: Q13426 and P12956 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13426 and P12956 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13426 and P12956 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13426 and P12956 |
Pubmed |
SVM Score :0.0 |
| NA |
|