| Interacting proteins: P49917 and P12956 |
Pubmed |
SVM Score :0.0 |
| Ku 70 , DNA PKcs , DNA Ligase 4 , Xrcc 4 ) were observed . ^^^ |
|
| Interacting proteins: P49917 and P12956 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P49917 and P12956 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P49917 and P12956 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P49917 and P12956 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P49917 and P12956 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P49917 and P12956 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P49917 and P12956 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P49917 and P12956 |
Pubmed |
SVM Score :0.0 |
| Several known human genes involved in DNA metabolism , such as the Ku 70 end binding protein and DNA ligase 4 , were identified , as well as a large number of previously unknown genes . ^^^ |
|
| Interacting proteins: P49917 and P12956 |
Pubmed |
SVM Score :0.0 |
| Aside from DNA PK , XRCC 4 and DNA ligase 4 , other proteins which play a role ( s ) in this repair pathway remain unknown ; DNA PK contains a catalytic subunit ( DNA PKcs ) and a DNA binding subunit ( Ku 70 and Ku 80 ) . ^^^ |
|
| Interacting proteins: P49917 and P12956 |
Pubmed |
SVM Score :0.0 |
| DNA double strand breaks formed during the assembly of antigen receptors or after exposure to ionizing radiation are repaired by proteins important for nonhomologous end joining that include Ku 86 , Ku 70 , DNA PK ( CS ) , Xrcc 4 , and DNA ligase 4 . ^^^ |
|
| Interacting proteins: P49917 and P12956 |
Pubmed |
SVM Score :0.0 |
| Any defect in one of the known components of the DNA repair / V ( D ) J recombination machinery ( Ku 70 , Ku 80 , DNA PKcs , XRCC 4 and DNA ligase 4 ) leads to abortion of the 5 ( D ) J rearrangement process , early block in both T and B cell maturation , and ultimately to severe combined immune deficiency ( SCID ) in several animal models . ^^^ |
|
| Interacting proteins: P49917 and P12956 |
Pubmed |
SVM Score :0.0 |
| Mammalian nonhomologous DNA end joining employs Ku 70 , Ku 80 , DNA dependent protein kinase catalytic subunit ( DNA PKcs ) , XRCC 4 , and DNA ligase 4 ( Lig 4 ) . ^^^ |
|
| Interacting proteins: P49917 and P12956 |
Pubmed |
SVM Score :0.0 |
| MEFs homozygous for mutations that inactivate either DNA ligase 4 ( Lig 4 ) or Ku 70 display dramatic genomic instability , even in the absence of exogenous DNA damaging agents . ^^^ |
|
| Interacting proteins: P49917 and P12956 |
Pubmed |
SVM Score :0.0 |
| Protein expression levels of the DNA dependent protein kinase ( DNA PK ) / Ku dependent nonhomologous DNA end joining proteins Xrcc 4 , DNA ligase 4 , Ku 70 , and Ku 86 in FA and normal extracts were indistinguishable , as were DNA dependent protein kinase and DNA end binding activities . ^^^ |
|
| Interacting proteins: P49917 and P12956 |
Pubmed |
SVM Score :0.0 |
| DNA double strand break ( DSB ) repair by non homologous end joining ( NHEJ ) in mammalian cells requires the Ku 70 Ku80 heterodimer , the DNA PK catalytic subunit DNA PKcs , as well as DNA ligase 4 and Xrcc 4 . ^^^ We have characterized NHEJ in Schizosaccharomyces pombe using an extrachromosomal assay and find that , as anticipated , it is Ku 70 and DNA ligase 4 dependent . ^^^ |
|
| Interacting proteins: P49917 and P12956 |
Pubmed |
SVM Score :0.0 |
| Ku 86 together with Ku 70 , DNA PKcs , XRCC 4 and DNA ligase 4 forms a complex involved in repairing DNA double strand breaks ( DSB ) in mammals . ^^^ |
|
| Interacting proteins: P49917 and P12956 |
Pubmed |
SVM Score :0.0 |
| DNA ligase 4 deficient cells are more resistant to ionizing radiation in the absence of Ku 70 : Implications for DNA double strand break repair . ^^^ |
|
| Interacting proteins: P49917 and P12956 |
Pubmed |
SVM Score :0.0 |
| Broken DNA ends are rejoined by non homologous end joining ( NHEJ ) pathways requiring the Ku proteins ( Ku 70 , Ku 80 ) , DNA ligase 4 and its associated protein Lif1 / Xrcc4 ( ref . 1 ) . ^^^ |
|
| Interacting proteins: P49917 and P12956 |
Pubmed |
SVM Score :0.0 |
| NHEJ can be catalyzed by mammalian whole cell extract in a reaction dependent upon DNA ligase 4 , Xrcc 4 , Ku 70 , Ku 80 , and DNA PKcs . ^^^ Cell extracts derived from Brca 1 deficient mouse embryonic fibroblasts exhibit reduced end joining activity independent of the endogenous protein amounts of DNA ligase 4 , Ku 80 , and Ku 70 . ^^^ |
|
| Interacting proteins: P49917 and P12956 |
Pubmed |
SVM Score :0.0 |
| Detailed examination indicates that the patient is defective neither in the known factors involved in NHEJ in mammals ( Ku 70 , Ku 80 , DNA dependent protein kinase catalytic subunit , Xrcc 4 , DNA ligase 4 , or Artemis ) nor in the Mre11 / Rad50 / Nbs1 complex , whose homologue in Saccharomyces cerevisiae functions in NHEJ . ^^^ |
|
| Interacting proteins: P49917 and P12956 |
Pubmed |
SVM Score :0.0 |
| We did not observe any abnormalities in expression level or migration pattern of the following NHEJ proteins in radiosensitive cancer cases : Ku 70 , Ku 80 , XRCC 4 , DNA Ligase 4 . ^^^ |
|
| Interacting proteins: P49917 and P12956 |
Pubmed |
SVM Score :0.0 |
| The deletion of the genes coding for Ku 70 or DNA ligase 4 , which are both obligatory constituents of the non homologous end joining ( NHEJ ) pathway , each resulted in a 50 % reduction of replication independent mutation frequency in haploid cells . ^^^ |
|
| Interacting proteins: P49917 and P12956 |
Pubmed |
SVM Score :0.0 |
| The main components of the NHEJ system in eukaryotes are the catalytic subunit of DNA protein kinase ( DNA PK ( cs ) ) , which is recruited by DNA Ku protein , a heterodimer of Ku 70 and Ku 80 , as well as XRCC 4 protein and DNA ligase 4 . ^^^ |
|
| Interacting proteins: P49917 and P12956 |
Pubmed |
SVM Score :0.0 |
| RESULTS : Using both supershift and Western analysis , the following proteins were identified in the DNA end binding complex : Ku 70 , Ku 80 , DNA dependent protein kinase catalytic subunit , DNA ligase 4 , 10 ray cross complementing protein 4 , meiotic recombination protein 11 ( Mre 11 ) , Werner ' s syndrome protein , Bloom ' s syndrome protein , p 53 , poly ( ADP ribose ) polymerase , replication protein A ( RPA ) 14 , and RPA 32 , ataxia telangiectasia mutant , c Abl , Rad 50 , Nijmegen breakage syndrome protein 1 ( NBS 1 ) , and DNA ligase 3 were not detected in the binding complex by any assay . ^^^ |
|
| Interacting proteins: P49917 and P12956 |
Pubmed |
SVM Score :0.0 |
| Unexpectedly , however , we found that nonhomologous end joining ( NHEJ ) , another DSB repair pathway , has no positive role in the relevant repair ; notably , DT 40 cell mutants lacking either of the NHEJ factors ( namely , Ku 70 , DNA dependent protein kinase catalytic subunit , and DNA ligase 4 ) were resistant to killing by CPT . ^^^ |
|
| Interacting proteins: P49917 and P12956 |
Pubmed |
SVM Score :0.0 |
| Western blot analysis indicated that the expression of NHEJ pathway components Ku 70 , XRCC 4 , and DNA ligase 4 was downregulated in NSCLC cells A 549 with Ad mda 7 treatment . ^^^ |
|
| Interacting proteins: P49917 and P12956 |
Pubmed |
SVM Score :0.0 |
| This band was also completely supershifted by the addition of antibodies to ATM , Ku 70 , DNA ligase 3 , Rpa 32 , Rpa 14 , DNA ligase 4 , XRCC 4 , WRN , BLM , RAD 51 and p 53 . ^^^ The density of one of the DNA EBCs predicted the radiosensitization caused by BRCA haploinsufficiency , and this band contains Ku 70 , ATM , DNA ligase 3 , Rpa 32 , Rpa 14 , DNA ligase 4 , XRCC 4 , WRN , BLM , RAD 51 and p 53 but not BRCA 1 or 2 . ^^^ |
|
| Interacting proteins: P49917 and P12956 |
Pubmed |
SVM Score :0.0 |
| The budding yeast Saccharomyces cerevisiae repairs DSB primarily by homologous recombination ( HR ) , despite the presence of the KU 70 , KU 80 , DNA ligase 4 and XRCC 4 homologues , essential factors of the mammalian non homologous end joining ( NHEJ ) machinery . ^^^ |
|
| Interacting proteins: P49917 and P12956 |
Pubmed |
SVM Score :0.0 |
| The core components of mammalian NHEJ are the catalytic subunit of DNA protein kinase ( DNA PK ( cs ) ) , Ku subunits Ku 70 and Ku 80 , Artemis , XRCC 4 and DNA ligase 4 . ^^^ |
|
| Interacting proteins: P49917 and P12956 |
Pubmed |
SVM Score :0.0 |
| DNA double strand breaks ( dsb ) during 5 ( D ) J recombination of T and B lymphocyte receptor genes are resolved by the non homologous DNA end joining pathway ( NHEJ ) including at least six factors : Ku 70 , Ku 80 , DNA PK ( cs ) , Artemis , Xrcc 4 , and DNA ligase 4 ( Lig 4 ) . ^^^ |
|
| Interacting proteins: P49917 and P12956 |
Pubmed |
SVM Score :0.0 |
| However , although Lig 4 deficiency causes late embryonic lethality , deficiency in DNA PK subunits ( Ku 70 , Ku 80 , and DNA PKcs ) does not . ^^^ |
|
| Interacting proteins: P49917 and P12956 |
Pubmed |
SVM Score :0.0 |
| Furthermore , roles for Ku 70 and Lig 4 were identified in suppressing DSB induced chromosomal rearrangements associated with gene conversion . ^^^ |
|
| Interacting proteins: P49917 and P12956 |
Pubmed |
SVM Score :0.0 |
| The non homologous end joining ( NHEJ ) pathway of DNA recombination is important for genomic stability in animal cells , since the absence of Ku 70 , Ku 80 , Lig 4 or Xrcc 4 results in non reciprocal translocation and chromosome fragmentation . ^^^ |
|
| Interacting proteins: P49917 and P12956 |
Pubmed |
SVM Score :0.0 |
| Twelve loci of selected DNA repair genes were genotyped by matrix assisted laser desorption / ionization time of flight mass spectrometry ( XRCC 2 Arg188His , XRCC 4 921G > T , XRCC 6 1796G > T , LIG 4 1977T / C , RAD 51 135G > C , 172G > T , RAD 52 2259C > T , LIG 1 551A > C , ERCC 1 8092A > C , 354C > T , hMLH 1 93G > A , and Ile219Val ) . ^^^ |
|
| Interacting proteins: P49917 and P12956 |
Pubmed |
SVM Score :0.0 |
| LOH was recurrently observed for 8 of the 9 studied genes ( KU 70 , KU 80 , XRCC 4 , LIG 4 , Artemis , MRE 11 , RAD 50 , NBS 1 ) but not for DNA PKcs . ^^^ |
|