Pubmed abstracts for Protein-Protein Interaction search result :

Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
The ML 8 tumour showed higher DNA ploidy . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
We have examined the concept of genomic instability in relation to the metastatic progression of low ( F 1 ) and high metastasis ( BL 6 , ML 8 ) clones of the B 16 mouse melanoma , by using a mutation assay , and DNA strand break repair and repair fidelity assays . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
The DNA dependent protein kinase ( DNA PK ) consists of three polypeptide components : Ku 70 , Ku 80 , and an approximately 350 kDa catalytic subunit ( p 350 ) . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
Three lines of evidence indicate that TATA binding protein ( TBP ) is additionally involved in the formation of CTCBF : ( 1 ) TBP can be detected in purified CTCBF ; ( 2 ) the addition of recombinant TBP stimulates formation of the CTCBF DNA complex ; and ( 3 ) antibodies directed against TBP interfere strongly with the formation of the specific protein DNA complex . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
Ku 80 protein forms heterodimer with the Ku 70 subunit to form a complex that possesses a DNA end binding activity . ^^^ However , introduction of human Ku 80 gene into the mutant lead to increased expression of Ku 70 protein and restored Ku 70 binding to DNA ends , suggesting that mutation of the Ku 80 gene affected the formation of Ku70 / Ku80 dimers and the stability of the Ku 70 protein . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
Transfection of full length cDNA encoding hamster Ku 86 into XR V15B cells resulted in a complete rescue of DNA end binding ( DEB ) activity and Ku 70 levels , suggesting that Ku 86 stabilizes the Ku 70 polypeptide . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
DNA dependent protein kinase ( DNA PK ) consists of three polypeptide subunits : Ku 70 , Ku 80 , and the DNA PK catalytic subunit ( DNA PKcs ) . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
Three proteins known to play a critical role in mammalian DNA double strand break repair and lymphoid 5 ( D ) J recombination are the autoantigens Ku 86 and Ku 70 and a 465 kDa serine / threonine protein kinase catalytic subunit ( DNA PKcs ) . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
Protein protein and protein DNA interaction regions within the DNA end binding protein Ku 70 Ku86 . ^^^ Little is known of which regions of Ku 70 and Ku 86 bind to each other to form the heterodimeric complex or of which regions are important for DNA end binding . ^^^ DNA end binding activity by the heterodimer requires the C terminal 40 kDa region of Ku 70 and the C terminal 45 kDa region of Ku 86 . ^^^ Leucine zipper like motifs in both subunits that have been suggested as the Ku 70 Ku86 interaction domains do not appear to be the sites of such interaction because these are dispensable for both assembly and DNA end binding . ^^^ On the basis of these studies , we have organized Ku 70 into nine sequence regions conserved between Saccharomyces cerevisiae , Drosophila melanogaster , mice , and humans ; only the C terminal three regions are essential for assembly ( amino acids [ aa ] 439 to 609 ) , and the C terminal four regions appear to be essential for DNA end binding ( aa 254 to 609 ) . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
Normal protein and transcription levels of Ku 70 and Ku 80 , as well as DNA PK activity , were found in six different AT cell lines , 1 4 h following exposure to ionizing radiation , timepoints where reduced and delayed transcriptional induction of WAF1 / CIP1 ( p 21 ) was observed . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
Saccharomyces cerevisiae Ku 70 potentiates illegitimate DNA double strand break repair and serves as a barrier to error prone DNA repair pathways . ^^^ Ku , a heterodimer of polypeptides of approximately 70 kDa and 80 kDa ( Ku 70 and Ku 80 , respectively ) , binds avidly to DNA double strand breaks ( DSBs ) . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
We have analyzed expression of Ku 70 , Ku 80 and DNA PKcs upon induction of differentiation in preB cells derived from wild type , severe combined immunodeficiency ( SCID ) and Rag 2 / mice . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
While the formation of the Ku 70 and Ku 80 complex is important for repair of DNA double strand breaks our data suggest that the 70 kDa subunit of Ku plays a role in regulating hsp 70 expression . . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
Ku is a heterodimer of polypeptides of approximately 70 and 80 kDa ( Ku 70 and Ku 80 , respectively ) that binds to DNA ends . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
DNA dependent protein kinase ( DNA PK ) consists of a 460 kDa subunit that contains the catalytic domain ( DNA PKcs ) complexed with two polypeptides of 70 kDa and 80 kDa ( Ku 70 and Ku 80 ) which comprise the Ku autoantigen . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
All mutants examined have low or undetectable levels of Ku 70 and Ku 80 protein , end binding , and DNA PK activities . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
The DNA dependent protein kinase ( DNA PK ) is a trimeric enzyme consisting of a 460 kDa catalytic subunit ( DNA PKcs ) and a heterodimeric regulatory complex called Ku , which is comprised of 70 ( Ku 70 ) and 86 ( Ku 80 ) kDa subunits . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
Genes essential for the end joining reaction include : XRCC 4 encoding a protein of unknown enzymatic function ; XRCC 5 and XRCC 6 encoding 86 and 70 kDa subunits of the Ku autoantigen , a DNA end binding protein that is also the regulatory subunit of DNA dependent protein kinase ( DNA PK ) ; and XRCC 7 encoding the catalytic subunit ( DNA PKcs ) of DNA PK . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
Ku 70 deficient embryonic stem cells have increased ionizing radiosensitivity , defective DNA end binding activity , and inability to support 5 ( D ) J recombination . 5 ( D ) J recombination requires both lymphoid specific and generally expressed enzymatic activities . ^^^ The Ku 70 protein is an additional component of DNA PK ; Ku 70 forms a heterodimer with Ku 80 to generate the DNA end binding component of the enzyme . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
Ku , consisting of an Mr 70 , 000 ( Ku 70 ) and an Mr 86 , 000 ( Ku 80 ) subunit , is a heterodimeric nuclear protein and is the DNA binding regulatory component of the mammalian DNA dependent protein kinase DNA PK . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
Ku 70 is required for DNA repair but not for T cell antigen receptor gene recombination In vivo . ^^^ Ku is a complex of two proteins , Ku 70 and Ku 80 , and functions as a heterodimer to bind DNA double strand breaks ( DSB ) and activate DNA dependent protein kinase . ^^^ The role of the Ku 70 subunit in DNA DSB repair , hypersensitivity to ionizing radiation , and 5 ( D ) J recombination was examined in mice that lack Ku 70 ( Ku 70 ( / ) ) . ^^^ Like Ku 80 ( / ) mice , Ku 70 ( / ) mice showed a profound deficiency in DNA DSB repair and were proportional dwarfs . ^^^ Our data shows , for the first time , that Ku 70 plays an essential role in DNA DSB repair , but is not required for TCR 5 ( D ) J recombination . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
The DNA PKcs gene encodes the 465 kDa catalytic subunit of DNA dependent protein kinase ( DNA PK ) , which associates with heterodimeric autoantigens Ku 70 and Ku 80 and exhibits protein kinase activity depending on DNA double strand breaks . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
Double strand break repair by Ku 70 requires heterodimerization with Ku 80 and DNA binding functions . ^^^ Heterodimers of the 70 and 80 kDa Ku autoantigens ( Ku 70 and Ku 80 ) activate the DNA dependent protein kinase ( DNA PK ) . ^^^ Mutations in any of the three subunits of this protein kinase ( Ku 70 , Ku 80 and DNA PKcs ) lead to sensitivity to ionizing radiation ( IR ) and to DNA double strand breaks , and 5 ( D ) J recombination product formation defects . ^^^ Here we show that the IR repair , DNA end binding and DNA PK defects in Ku 70 / embryonic stem cells can be counteracted by introducing epitope tagged wild type Ku 70 cDNA . ^^^ Truncations and chimeras of Ku 70 were used to identify the regions necessary for DNA end binding and IR repair . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
Ku 70 , Ku 80 , and DNA PKcs are subunits of the DNA dependent protein kinase ( DNA PK ) , an enzyme implicated in DNA double stranded break repair and 5 ( D ) J recombination . ^^^ These unanticipated features of the Ku 70 deficient phenotype with respect to lymphocyte development and 5 ( D ) J recombination may reflect differential functions of the three DNA PK components . . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
Recent studies suggest that the SCID defect lies in the gene encoding the catalytic subunit of DNA dependent protein kinase ( DNA PK ; refs 6 8 ) , a nuclear protein made up of the Ku 70 and Ku 86 subunits as well as the large catalytic subunit , DNA PKcs . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
We demonstrate here that the products of the catalytic subunit of DNA dependent protein kinase ( DNA PKcs ) and its regulatory subunits ( Ku 70 and Ku 80 ) are present at normal levels and possess functional activity . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
Ku , the DNA binding component of DNA dependent protein kinase ( DNA PK ) , is a heterodimer composed of 70 and 86 kDa subunits , known as Ku 70 and Ku 80 respectively . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
DNA restriction analysis revealed that the prophages in C 10 , ML 8 , and NCK 203 had identical restriction patterns which were different from that of phi r1t . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
The heterodimeric Ku protein , which comprises a 86 kDa ( Ku 86 ) amd a 70 kDa ( Ku 70 ) subunits , is an abundant nuclear DNA binding protein which binds in vitro to DNA termini without sequence specificity . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
Ku 70 and Ku 80 form a DNA end binding complex required for DNA double strand break repair and 5 ( D ) J recombination . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
The mammalian Ku 70 and Ku 86 proteins form a heterodimer that binds to the ends of double stranded DNA in vitro and is required for repair of radiation induced strand breaks and 5 ( D ) J recombination [ 1 , 2 ] . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
Interestingly , either Ku 70 or Ku 80 deficient cell lines , but not mutant cell lines of the DNA PK catalytic sub unit ( DNA PKcs ) , were found to be hypersensitive to the effects of etoposide VP 16 . ^^^ Mutational analysis of introduced Ku 70 cDNAs into murine embryonic stem cells deleted for Ku 70 ( / ) showed that mutants where heterodimerization and DNA binding functions of Ku were disrupted , also blocked the restoration of etoposide resistance . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
The other components of the DNA PK Ku autoantigen complex , Ku 70 and Ku 80 , were 3 fold increased and unchanged , respectively . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
Saccharomyces Ku 70 , mre11 / rad50 and RPA proteins regulate adaptation to G2 / M arrest after DNA damage . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
The DNA dependent protein kinase is a mammalian protein complex composed of Ku 70 , Ku 80 , and DNA PKcs subunits that has been implicated in DNA double strand break repair and 5 ( D ) J recombination . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
The DNA dependent protein kinase ( DNA PK ) consists of Ku 70 , Ku 80 , and a large catalytic subunit , DNA PKcs . ^^^ We conclude that Ku 70 and Ku 80 may have functions in 5 ( D ) J recombination and DNA repair that are independent of DNA PKcs . . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
From the analysis of defective rodent mutants , four proteins ( Ku 70 , Ku 80 , DNA PKcs and Xrcc 4 ) that function in this process in mammalian cells have been identified . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
These factors include components of DNA dependent protein kinase ( DNA PK ) , namely DNA PKcs and the Ku heterodimer , Ku 70 Ku80 , and XRCC 4 . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
Intermolecular ligation was found to be accurate and to depend on DNA ligase IV / Xrcc4 and requires Ku 70 , Ku 86 , and DNA PKcs , the three subunits of the DNA activated protein kinase DNA PK . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
NF 90 and NF 45 associate with the DNA dependent protein kinase catalytic subunit and the DNA targeting subunits Ku 80 and Ku 70 ( N . ^^^ The purine box transcriptional regulator in 16HBE cells likely comprises NF 45 , NF 90 , Ku 80 , Ku 70 , and the DNA dependent protein kinase catalytic subunit . . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
Previous studies have suggested that ionizing radiation causes irreparable DNA double strand breaks in mice and cell lines harboring mutations in any of the three subunits of DNA dependent protein kinase ( DNA PK ) ( the catalytic subunit , DNA PKcs , or one of the DNA binding subunits , Ku 70 or Ku 86 ) . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
DNA dependent protein kinase phosphorylation sites in Ku 70 / 80 heterodimer . ^^^ Ku antigen is composed of 70 and 82 kDa subunits ( Ku 70 and Ku 80 , respectively ) that together bind with high affinity to ends of double stranded DNA and other DNA structures in vitro . ^^^ When bound to DNA , the Ku 70 / 80 heterodimer enhances the kinase activity of the catalytic subunit of the DNA dependent protein kinase , DNA PKcs . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
Several known human genes involved in DNA metabolism , such as the Ku 70 end binding protein and DNA ligase 4 , were identified , as well as a large number of previously unknown genes . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
The repressor which binds the 75 GATA motif of the GPB promoter contains Ku 70 as the DNA binding subunit . ^^^ Here , we have identified the Ku 70 protein as a candidate GPB repressor DNA binding subunit through the screening of a human HeLa expression library using the 75 GATA sequence as bait ( one hybrid method ) . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
In addition , the presence of Ku 70 and La protein as NMPs suggests that the NM can be functionally active in association with DNA or RNA . . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
This protein contributes to the repair of DNA double strand breaks ( DSBs ) by assembling broken ends of DNA molecules in combination with the DNA binding factors Ku 70 and Ku 80 . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
Aside from DNA PK , XRCC 4 and DNA ligase 4 , other proteins which play a role ( s ) in this repair pathway remain unknown ; DNA PK contains a catalytic subunit ( DNA PKcs ) and a DNA binding subunit ( Ku 70 and Ku 80 ) . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
DNA PKcs , Ku 80 , and Ku 70 mRNAs were expressed in all normal tissues with relatively little variation in levels . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
Spontaneous and mitomycin C induced SCE levels were significantly reduced for chicken DT 40 B cells lacking the key HR genes RAD 51 and RAD 54 but not for nonhomologous DNA end joining ( NHEJ ) defective KU 70 ( / ) cells . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
Targeted disruption of the genes encoding Ku 70 and Ku 80 has identified significant differences between Ku deficient mice and DNA PKcs deficient mice . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
The heterodimeric Ku protein , composed of 86 kDa ( Ku 80 ) and 70 kDa ( Ku 70 ) subunits , is the DNA targeting component of DNA dependent protein kinase ( DNA PK ) , which plays a critical role in mammalian DNA double strand breaks repair . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
The complex has a strongly preferred orientation , with Ku 70 DNA backbone contacts located proximal and Ku 80 DNA backbone contacts located distal to the free end . ^^^ Results are consistent with a model where the Ku 70 and Ku 80 subunits contact the major and minor grooves of DNA , respectively . . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
Interaction with Ku 70 and DNA end binding are important for Ku 80 function in vivo , since Ku 80 mutants lacking DNA end binding activity were unable to restore radiation resistance in Ku 80 deficient fibroblast cell lines . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
Ku deficient cells were more sensitive to bleomycin induced DNA fragmentation and exhibited a higher level of c jun NH 2 kinase / stress activated PK activity than wild type cells , whereas R 7080 6 cells overexpressing both human Ku 70 and Ku 80 were resistant to bleomycin induced apoptosis and exhibited a lower level of c jun NH 2 kinase / stress activated PK activity . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
DNA double strand breaks formed during the assembly of antigen receptors or after exposure to ionizing radiation are repaired by proteins important for nonhomologous end joining that include Ku 86 , Ku 70 , DNA PK ( CS ) , Xrcc 4 , and DNA ligase 4 . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
DNA PK is a nuclear serine / threonine kinase that comprises three components : a catlytic subunit ( DNA PKCS ) and two regulatory subunits , DNA binding proteins , Ku 80 and Ku 70 . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
Moreover , we show that irradiated cells deficient for Ku 70 are incapable of targeting Mre 11 to subnuclear foci that may represent DNA repair complexes . ^^^ When present , Ku 70 destines DSBs for NHEJ by binding to DNA ends and attracting other factors for NHEJ , including Mre 11 ; when absent , it allows participation of DNA ends and Mre 11 in the meiotic HR pathway . . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
The highly expressed Ku heterodimer , composed of 70 and 80 K ( d ) subunits ( Ku 70 and Ku 80 ) , is the high affinity DNA binding component of the DNA dependent protein kinase . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
Ku antigen is a complex of Ku 70 and Ku 80 subunits and plays an important role in not only DNA double strand breaks ( DSB ) repair and 5 ( D ) J recombination , but also in growth regulation . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
The activity is elevated in late passaged and senescent cells , and the cleaved 69 kDa product seems able to form complex with Ku 70 to bind DNA ends . ^^^ In fact , the levels of Ku 86 , Ku 70 and DNA end binding activity of Ku remained unchanged during replicative senescence . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
DNA PK plays a major role in the repair of double strand breaks induced in DNA after exposure to ionizing radiation as shown by the extreme radiosensitivity of cells with mutations in Ku 86 , Ku 70 or DNA PKcs genes . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
DNA damage recognition and repair pathways involve several nuclear proteins , as double strand breaks are firstly recognized by proteins displaying helicase activity , such as ku 70 / 80 , and then repair is carried out under the control of other proteins . ^^^ Maintenance of DNA integrity is fundamental for normal immune functions , as suggested by the lack of 5 ( D ) J recombination in lymphocytes of knock out mice deficient in ku 70 or ku 80 protein . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
Ku 70 and Ku 80 have been shown to form a heterodimeric complex that can bind tightly to free DNA ends and activate the protein kinase DNA PKcs . ^^^ Further , we uncovered that the postirradiation up regulation of Ku 70 utilizes a mechanism that is dependent on both p 53 and damage response protein kinase ATM ( ataxia telangiectasia mutated ) ; however , the activation of DNA PK does not require Ku 70 up regulation . ^^^ These findings suggest that Ku 70 up regulation provides the cell with a means of assuring either proper DNA repair or an appropriate response to DNA damage independent of DNA PKcs activation . . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
Any defect in one of the known components of the DNA repair / V ( D ) J recombination machinery ( Ku 70 , Ku 80 , DNA PKcs , XRCC 4 and DNA ligase 4 ) leads to abortion of the 5 ( D ) J rearrangement process , early block in both T and B cell maturation , and ultimately to severe combined immune deficiency ( SCID ) in several animal models . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
Using Western blots we measured , in non irradiated cells , the basal expression levels of ATM , Rad 1 and Hus 1 , involved in the control of cellular DNA damage checkpoints , together with DNA PKcs , Ku 70 , Ku 80 ; XRCC 4 , ligaseIV and Rad 51 , involved in radiation induced DSB repair . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
Mammalian nonhomologous DNA end joining employs Ku 70 , Ku 80 , DNA dependent protein kinase catalytic subunit ( DNA PKcs ) , XRCC 4 , and DNA ligase 4 ( Lig 4 ) . ^^^ Herein , we show that Ku 70 and Ku 80 deficiency but not DNA PKcs deficiency results in dramatically increased death of developing embryonic neurons in mice . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
Its regulatory subunit Ku ( dimer of the Ku 70 and Ku 80 protein ) binds to DNA and recruits the kinase catalytic sub unit , DNA PKcs . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
The three other proteins were identified as subunits of the DNA dependent protein kinase ( DNA PK ) holoenzyme : its DNA binding regulatory heterodimers consisting of Ku 70 and Ku 86 , and the 460 kDa catalytic subunit , DNA PK ( CS ) . ^^^ DNA PK that was ' pulled down ' by DBD ( PR ) on the affinity resin was able to ( 1 ) autophosphorylate Ku 70 , Ku 86 , and DNA PK ( CS ) , ( 2 ) transphosphorylate DBD ( PR ) , and ( 3 ) phosphorylate a DNA PK specific p 53 peptide substrate . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
MEFs homozygous for mutations that inactivate either DNA ligase 4 ( Lig 4 ) or Ku 70 display dramatic genomic instability , even in the absence of exogenous DNA damaging agents . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
We reisolated CLU / XIP8 by yeast two hybrid analyses using as bait the DNA double strand break repair protein Ku 70 . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
This inhibition is associated with decreased TNFR associated factor 2 binding to CD 40 , decreased NF kappaB binding to the CD 40 responsive element of the Cgamma 3 promoter , decreased Igamma 3 Cgamma3 germline gene transcription , and decreased expression of Ku 70 , Ku 80 , DNA protein kinase , switch associated protein 70 , and Msh 2 CSR associated transcripts . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
Peptide sequence analysis indicates that the two proteins are identical to Ku 70 and Ku 80 , a heterodimer involved in double strand DNA break repair by non homologous DNA end joining . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
Effects of HDF 1 ( Ku 70 ) and HDF 2 ( Ku 80 ) on spontaneous and DNA damage induced intrachromosomal recombination in Saccharomyces cerevisiae . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
Using a combination of alanine scanning , polymer blot analysis , and photoaffinity labeling , we have identified poly ( ADP ribose ) binding sites in the following proteins : p 53 , p 21 ( CIP1 / WAF1 ) , xeroderma pigmentosum group A complementing protein , MSH 6 , DNA ligase 3 , XRCC 1 , DNA polymerase epsilon , DNA PK ( CS ) , Ku 70 , NF kappaB , inducible nitric oxide synthase , caspase activated DNase , and telomerase . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
Ku 70 can translocate to the nucleus independent of Ku 80 translocation and DNA PK autophosphorylation . ^^^ Human Ku 70 , when transfected , accumulated within the nuclei of hamster xrs 6 cells which had undetectable DNA PK activity and Ku 80 . ^^^ Ku 70 and Ku 80 mutants of DNA PK phosphorylation sites showed normal heterodimerization and nuclear translocation . ^^^ These findings also support the idea that Ku 70 can translocate to the nucleus independent of DNA PK autophosphorylation . . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
Ku is a heterodimer of Ku 70 and Ku 86 that binds to double stranded DNA breaks ( DSBs ) , activates the catalytic subunit ( DNA PKcs ) when DNA is bound , and is essential in DSB repair and 5 ( D ) J recombination . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
In contrast to HP1alpha , Ku 70 did not repress transcriptional activity of the reporter gene when tethered to DNA after transfection to mammalian cells . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
While the Ku complex , comprised of Ku 70 and Ku 80 , is primarily involved in the repair of DNA double strand breaks , it is also believed to participate in additional cellular processes . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
Protein expression levels of the DNA dependent protein kinase ( DNA PK ) / Ku dependent nonhomologous DNA end joining proteins Xrcc 4 , DNA ligase 4 , Ku 70 , and Ku 86 in FA and normal extracts were indistinguishable , as were DNA dependent protein kinase and DNA end binding activities . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
DNA PKcs , Ku 70 , Ku 85 , Xrcc 4 , and Nbs 1 play a critical role in DNA DSB repair . ^^^ METHODS : We immunohistochemically investigated the expression of DNA PKcs , Ku 70 , Ku 85 , Xrcc 4 , and Nbs 1 in 134 specimens from various normal and tumor tissues with different radiosensitivity . ^^^ RESULTS AND CONCLUSION : Immunopositivity to Ku 70 , Ku 85 , DNA PKcs , Xrcc 4 , and Nbs 1 was found in all tumor tissues examined . ^^^ The staining for Ku 70 , Ku 85 , and DNA PKcs was nuclear ; but , for Xrcc 4 and Nbs 1 , it was nuclear and cytoplasmic . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
DNA double strand break ( DSB ) repair by non homologous end joining ( NHEJ ) in mammalian cells requires the Ku 70 Ku80 heterodimer , the DNA PK catalytic subunit DNA PKcs , as well as DNA ligase 4 and Xrcc 4 . ^^^ We have characterized NHEJ in Schizosaccharomyces pombe using an extrachromosomal assay and find that , as anticipated , it is Ku 70 and DNA ligase 4 dependent . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
Additionally , we observed an increased interaction of PCNA with the Ku 70 and Ku 80 heterodimer after DNA damage , suggestive of a role for PCNA in the non homologous end joining repair pathway of DNA strand breaks . . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
However , although Lig 4 deficiency causes late embryonic lethality , deficiency in DNA PK subunits ( Ku 70 , Ku 80 , and DNA PKcs ) does not . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
Ku 86 together with Ku 70 , DNA PKcs , XRCC 4 and DNA ligase 4 forms a complex involved in repairing DNA double strand breaks ( DSB ) in mammals . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
The mutant Dmblm phenotypes were partially rescued by an extra copy of the DNA repair gene Ku 70 , indicating that the two genes functionally interact in vivo . . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
Five components function in this pathway , of which three ( Ku 70 , Ku 80 , and the DNA dependent protein kinase catalytic subunit [ DNA PKcs ] ) constitute a complex termed DNA dependent protein kinase ( DNA PK ) . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
The complex includes the ku heterodimer , which comprises ku 70 and ku 80 subunits , that binds DNA termini of breaks without sequence specificity , and the catalytic subunit DNA PKCS : The activation of the DNA PK complex was studied in 10 irradiated peripheral blood mononuclear cells ( PBMC ) from subjects of different ages . ^^^ Radiation induced changes in the DNA binding activity of the ku heterodimer , and in the concentrations of ku 70 , ku 80 , DNA PKcs and phosphorylated ku 80 were determined in nuclear and cytoplasmic extracts . ^^^ The radiation induced activation of the DNA PK complex may result from the increased concentrations of ku 80 and DNA PKcs in the cytoplasm of PBMC from young , but not from elderly subjects , leading to a higher concentration of phosphorylated ku 80 which readily migrates to the nucleus where , after dimerization with ku 70 , binds to DNA breaks . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
In vitro , DNA PK undergoes phosphorylation of all three protein subunits ( DNA PK catalytic subunit , Ku 70 and Ku 80 ) and phosphorylation correlates with inactivation of the serine / threonine protein kinase activity of DNA PK . ^^^ In vivo phosphorylation of DNA PKcs , Ku 70 , and Ku 80 was observed when cells were labeled with [ ( 32 ) P ] inorganic phosphate in the presence of the protein phosphatase inhibitor , okadaic acid . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
Early decrease in dna repair proteins , Ku 70 and Ku 86 , and subsequent DNA fragmentation after transient focal cerebral ischemia in mice . ^^^ BACKGROUND AND PURPOSE : Ku 70 and Ku 86 , multifunctional DNA repair proteins , bind to broken DNA ends , including double strand breaks , and trigger a DNA repair pathway . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
The three dimensional structure of the C terminal DNA binding domain of human Ku 70 . ^^^ The proteins Ku 70 ( 69 . 8 kDa ) and Ku 80 ( 82 . 7 kDa ) form a heterodimeric complex that is an essential component of the nonhomologous end joining DNA double strand break repair pathway in mammalian cells . ^^^ Ku 70 , which is mainly responsible for the DNA binding activity of the Ku heterodimer , contains two DNA binding domains . ^^^ We have solved the solution structure of the Ku 80 independent DNA binding domain of Ku 70 encompassing residues 536 609 using nuclear magnetic resonance spectroscopy . ^^^ Residues 561 609 of Ku 70 form a well defined structure with 3 alpha helices and also interact with DNA . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
The heterodimeric Ku protein ( composed of the Ku 86 and Ku 70 sub units ) is a nuclear protein which binds to DNA termini without sequence specificity . ^^^ We further demonstrate that immunoprecipitation of Ku is impaired in extracts from differentiated cells by using two antibodies that recognize epitopes within the C terminus DNA binding domains of Ku 70 and Ku 86 proteins . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
The Ku heterodimer ( Ku 70 and Ku 80 subunits ) contributes to genomic integrity through its ability to bind DNA double strand breaks and facilitate repair by the non homologous end joining pathway . ^^^ Ku 70 and Ku 80 share a common topology and form a dyad symmetrical molecule with a preformed ring that encircles duplex DNA . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
DNA repair by nonhomologous end joining ( NHEJ ) relies on the Ku 70 : Ku 80 heterodimer in species ranging from yeast to man . ^^^ Here , we show that Ku 70 , Ku 80 , and DNA PKcs , with which Ku interacts , associate in vivo with telomeric DNA in several human cell types , and we show that these associations are not significantly affected by DNA damaging agents . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
In this article , we demonstrate that GAS 16 results from an alternative splicing process of the Ku 70 binding protein 3 ( KUB 3 ) that is essential for DNA double strand break repair . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
DNA PK comprises a catalytic subunit ( DNA PKcs ) and a DNA binding Ku 70 and Ku 80 heterodimer . ^^^ DNA PKcs deficiency caused a DSB repair defect that was , unexpectedly , suppressed by KU 70 disruption . ^^^ To test whether direct interference by Ku 70 with HR might explain the Ku 70 ( / ) / DNA PKcs ( / / ) radioresistance , we monitored HR activities directly in Ku and DNA PKcs deficient cells . ^^^ Significantly , abrogation of HR activity by targeting RAD 54 in Ku 70 ( / ) or DNA PKcs ( / / ) cells caused extreme radiosensitivity , suggesting that the relative radioresistance seen with loss of Ku 70 was because of HR dependent repair pathways . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
Nonhomologous endjoining was investigated by determining the levels of Ku 70 and Ku 86 proteins and DNA PK activity . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
Neuronal expression of the DNA repair protein Ku 70 after ischemic preconditioning corresponds to tolerance to global cerebral ischemia . ^^^ Ku 70 / 86 , multifunctional DNA repair proteins , bind to broken DNA ends and trigger a DNA repair pathway . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
DNA ligase 4 deficient cells are more resistant to ionizing radiation in the absence of Ku 70 : Implications for DNA double strand break repair . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
DNA dependent protein kinase ( DNA PK ) , consisting of a catalytic subunit ( DNA PKcs ) and the Ku 70 and Ku 86 proteins , participates in the repair of DNA double strand breaks ( DSBs ) . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
Broken DNA ends are rejoined by non homologous end joining ( NHEJ ) pathways requiring the Ku proteins ( Ku 70 , Ku 80 ) , DNA ligase 4 and its associated protein Lif1 / Xrcc4 ( ref . 1 ) . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
XRCC 5 ( also known as Ku 80 ) is a component of the DNA dependent protein kinase ( DNA PK ) , existing as a heterodimer with G22P1 ( also known as Ku 70 ) . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
DNA dependent protein kinase ( DNA PK ) is composed of a 460 kDa catalytic subunit and the regulatory subunits Ku 70 and Ku 80 . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
We find that the HDR frequency is enhanced in Ku 70 ( / ) , XRCC 4 ( / ) , and DNA PKcs ( / ) cells , with the increase being particularly striking in Ku 70 ( / ) cells . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
Ku , composed of 70kDa ( ku 70 ) and 86kDa ( ku 80 ) proteins , is the DNA targeting subunit of the DNA dependent serine / threonine kinase ( DNA PK ) , which plays a crucial role in DNA double strand break recognition and repair in mammalian cells . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
Functional inactivation in rodents of either Ku 86 or Ku 70 , which form the heterodimeric DNA end binding subunit of the DNA dependent protein kinase complex , is nevertheless compatible with viability . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
The DNA end binding protein Ku ( an equimolar mixture of Ku 70 , now known as G22P1 , and Ku 86 , now known as XRCC 5 ) did not alter the resistance of the 3 ' phosphoglycolate substrates , but the protein modulated the susceptibility of 3 ' hydroxyl substrates , allowing DNase 3 to remove a 3 ' overhang but inhibiting digestion of the double stranded portion of the substrate . . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
The autoantigen Ku , composed of subunits Ku 70 and Ku 86 , is necessary for repair of DNA double strand breaks by nonhomologous end joining . ^^^ A central domain of Ku 70 containing its DNA binding and heterodimerization regions and its nuclear localization signal shows that this alone is sufficient for the observed mobility of Ku 70 GFP and its association with nuclear matrix . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
OBJECTIVE : Since the central hallmarks of human multiple myeloma ( MM ) are abnormalities in immunoglobulin ( Ig ) gene rearrangement , IgH class switching , and DNA damage repair , and since Ku 86 and Ku 70 proteins are central to these processes , aberrant Ku function may play a role in MM pathogenesis . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
In cells with segmented nuclei , continued DNA replication was observed , along with activation and redistribution of Ku 70 , suggestive of non homologous DNA end joining . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
Microarray analysis revealed that DNA repair associated genes , i . e . , XRCC 5 , XRCC 6 , ERCC 5 , hMLH 1 were over expressed in three fold cisplatin resistant cell line , KFr as compared to cisplatin sensitive parental cell line , KF . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
Differential expression of DNA nonhomologous end joining proteins Ku 70 and Ku 80 in melanoma progression . ^^^ Ku 70 and Ku 80 heterodimers function as regulatory subunits of the DNA dependent protein kinase and play a very important role in the repairing of DNA double strand breaks . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
DNA PK is a heterotrimeric protein complex comprised of two components : a large catalytic subunit , Prkdc , with serine / threonine kinase activity and a DNA targeting component , G22p1 and Xrcc 5 . ^^^ In previous report , we showed that approximately 80 % of the G22p1 and Xrcc 5 proteins were observed in the cytoplasm of rat fibroblasts , and that nuclear translocation of the proteins from the cytoplasm is important for the repair of DNA DSBs . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
It is a three protein complex consisting of a 470 kDa catalytic subunit ( DNA PKcs ) and the regulatory DNA binding subunits , Ku heterodimer ( Ku 70 and Ku 80 ) . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
NHEJ can be catalyzed by mammalian whole cell extract in a reaction dependent upon DNA ligase 4 , Xrcc 4 , Ku 70 , Ku 80 , and DNA PKcs . ^^^ Cell extracts derived from Brca 1 deficient mouse embryonic fibroblasts exhibit reduced end joining activity independent of the endogenous protein amounts of DNA ligase 4 , Ku 80 , and Ku 70 . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
Increased telomere length and hypersensitivity to DNA damaging agents in an Arabidopsis KU 70 mutant . ^^^ In other organisms the KU 70 gene has been shown to play a role in the repair of DNA damage induced by ionising radiation ( IR ) and by radiomimetic chemicals such as methylmethane sulfonate ( MMS ) . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
Identification of Ku 70 and Ku 80 homologues in Arabidopsis thaliana : evidence for a role in the repair of DNA double strand breaks . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
Blimp 1 also inhibited immunoglobulin class switching by blocking expression of AID , Ku 70 , Ku 86 , DNA PKcs , and STAT 6 . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
In AML samples , sensitivity to DNA cross linking alkylating agents correlated with Ku 86 ( r= 0 . 7512 , p=0 . 0031 ) and Ku 70 ( r= 0 . 6134 , p=0 . 0258 ) expression . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
In yeast and mammals , the exposed ends of damaged DNA are bound with high affinity by a dimer of Ku 70 and Ku 80 proteins , which protects the ends from exonucleases and juxtaposes the two ends of the DSB , independent of sequence homology . ^^^ Here we report the functional characterization of Ku 70 and Ku 80 from Arabidopsis thaliana , and demonstrate that AtKu 80 and AtKu 70 form a heterodimer with DNA binding activity that is specific for DNA ends . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
DNA PK is composed of a large catalytic subunit ( DNA PKcs ) and a heterodimer of Ku 70 and Ku 80 that assemble on the ends of double stranded DNA to form an active serine / threonine protein kinase complex . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
On the basis of telomere lengths of mutant combinations of various checkpoint related proteins ( Rad 1 , Rad 3 , Rad 9 , Rad 17 , Rad 26 , Hus 1 , Crb 2 , Chk 1 , Cds 1 ) , Tel 1 , a telomere binding protein ( Taz 1 ) , and DNA repair proteins ( Ku 70 , Rad 32 ) , we conclude that Rad3 / Rad26 and Tel1 / Rad32 represent two pathways required to maintain telomeres and prevent chromosome circularization . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
LOH was identified in the specific coding region of DNA PK in 39 . 29 % for the DNA PK catalytic subunit ( cs ) , 17 . 86 % for Ku 70 , and only 7 . 14 % for Ku 80 . ^^^ Ku 70 and DNA PKcs were almost identical in terms of immunoreactivity . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
Ku 70 is a subunit of DNA protein kinase complex and involved in diverse intranuclear events including the repair of double stranded DNA breaks . ^^^ The presented data indicate that the distribution of Ku 70 in cells of human tissues is closely associated with the cell cycle , cellular differentiation , nuclear shape and the process of repair of DNA damage caused by IR . . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
The gels were then processed for Western blotting using the Ku 70 , Ku 86 and DNA PKcs antibodies . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
These results demonstrate that Ku 70 and Ku 80 expression is mediated by constitutively activated NF kappaB and constitutively expressed COX 2 in gastric cancer cells and that the high Ku DNA end binding activity contributes to cell proliferation . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
The DNA repair protein Ku ( a heterodimer of Ku 70 and Ku 80 subunits ) associates with mammalian telomeres and contributes to telomere maintenance . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
DNA PK is composed of a large catalytic subunit , DNA PKcs , and a heterodimer of Ku 70 and Ku 80 subunits . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
OBJECTIVE : To examine the expression of DNA dependent protein kinase ( DNA PK ) ; including DNA PKcs , Ku 70 and Ku 80 in human neuroectodermal tumor cells with or without retroviral DNA integration . ^^^ RESULTS : The expression of DNA PKcs was similar in the cells with or without retroviral DNA integration , but the expression of Ku ( both mRNA and protein for Ku 70 and only protein for Ku 80 ) was higher in cells with retroviral DNA integration than in cells without retroviral DNA integration . ^^^ Ku 70 may play an important role in the regulation of Ku function in response to retroviral DNA integration . . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
Ku protein , a heterodimer of 70kDa ( Ku 70 ) and 86kDa ( Ku 86 ) polypeptides , is involved in non homologous DNA end joining ( NHEJ ) of DNA double strand break repair and 5 ( D ) J recombination in combination with the catalytic component of DNA dependent protein kinase ( p 470 ) . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
In analyzing the DNA repair capacity of C 70 and C 200 , an altered expression of the DNA dependent protein kinase ( DNA PK ) complex ( catalytic subunit DNA PKcs , and the heterodimers Ku 70 and Ku 80 ) was found . ^^^ In C 70 and C 200 cells , DNA PKcs was overexpressed at both the transcript and protein levels , whereas amounts of Ku 70 and Ku 80 were higher only at the level of protein expression . ^^^ Coimmunoprecipitation of Ku 70 after TLK 286 treatment of purified DNA PK and C 70 cells showed a drug induced destabilization of the protein protein interaction between the catalytic subunit and the Ku heterodimer . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
Suppression of a DNA double strand break repair gene , Ku 70 , increases radio and chemosensitivity in a human lung carcinoma cell line . ^^^ Ku 70 protein , cooperating with Ku 80 and DNA dependent protein kinase ( DNA PK ) catalytic subunit ( DNA PKcs ) , is involved in DNA double strand break ( DNA DSB ) repair and 5 ( D ) J recombination . ^^^ The antisense Ku 70 increased the chemosensitivity of the cells to some DNA damaging agents such as bleomycin and methyl methanesulfonate , but not to cisplatin , mitomycin C , and paclitaxel . ^^^ This system provides us with partial suppression of Ku 70 , and will be a useful experimental model for investigating the physiological roles of the DNA DSB repair gene . . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
Analysis by mass spectrometric protein sequencing revealed a DNA dependent protein kinase ( DNA PK ) complex including its catalytic subunit and regulatory subunits , Ku 70 and Ku 86 . ^^^ DNA PK phosphorylates TRBP at its C terminal region , which directly interacts with Ku 70 but not Ku 86 in vitro . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
Cells of mouse knockout cell lines for Ku 80 ( now known as Xrcc 5 ) , Ku 70 ( now known as G22p1 ) , DNA PKcs ( now known as Prkdc ) and PARP ( now known as Adprt ) were synchronized in G 1 phase and exposed to very low fluences of alpha particles . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
Ku is a heterodimeric protein consisting of Ku 70 and Ku 86 , and it is thought to be the first component to bind to a broken double strand DNA end . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
Function of DNA protein kinase catalytic subunit during the early meiotic prophase without Ku 70 and Ku 86 . ^^^ All components of the double stranded DNA break ( DSB ) repair complex DNA dependent protein kinase ( DNA PK ) , including Ku 70 , Ku 86 , and DNA PK catalytic subunit ( DNA PKcs ) , were found in the radiosensitive spermatogonia . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
Detailed examination indicates that the patient is defective neither in the known factors involved in NHEJ in mammals ( Ku 70 , Ku 80 , DNA dependent protein kinase catalytic subunit , Xrcc 4 , DNA ligase 4 , or Artemis ) nor in the Mre11 / Rad50 / Nbs1 complex , whose homologue in Saccharomyces cerevisiae functions in NHEJ . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
Ku 70 is a 70K subunit of the Ku complex , which has an important role in DNA double strand break ( DSB ) repair in the nucleus . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
These results suggest that in addition to its previously recognized DNA repair activity in the nucleus , Ku 70 has a cytoprotective function in the cytosol that controls the localization of Bax . . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
Six components function in this pathway , of which three ( Ku 70 , Ku 86 , and DNA PKcs ) constitute a protein complex known as DNA dependent protein kinase ( DNA PK ) . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
We did not observe any abnormalities in expression level or migration pattern of the following NHEJ proteins in radiosensitive cancer cases : Ku 70 , Ku 80 , XRCC 4 , DNA Ligase 4 . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
The non homologous end joining ( NHEJ ) pathway of DNA recombination is important for genomic stability in animal cells , since the absence of Ku 70 , Ku 80 , Lig 4 or Xrcc 4 results in non reciprocal translocation and chromosome fragmentation . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
The intracellular distribution and amount of the DNA dependent protein kinase ( DNA PK ) complex components ( ku 70 , ku 80 , DNA PKcs ) , which are involved in the recognition and repair of DNA breaks caused by ionizing radiations , 5 ( D ) J recombination and isotype switching , was assessed in nai ; ve and memory T cells from young subjects . ^^^ While the expression of ku 70 and ku 80 was at comparable levels in both T cell subsets , DNA PKcs , phosphorylated ku 80 , and DNA binding of ku 70 / 80 were mostly evident in nai ; ve but negligible or absent in memory T cells . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
The deletion of the genes coding for Ku 70 or DNA ligase 4 , which are both obligatory constituents of the non homologous end joining ( NHEJ ) pathway , each resulted in a 50 % reduction of replication independent mutation frequency in haploid cells . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
Because single nucleotide polymorphisms ( SNPs ) are the most subtle genetic variation in the genome , to examine these hypotheses , we have genotyped 30 SNPs in all five NHEJ genes ( Ku 70 , Ku 80 , DNA PKcs , Ligase 4 , and XRCC 4 ) in 254 primary breast cancer patients and 379 healthy controls . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
TdT synthesizes the N region in concert with many proteins including DNA PKcs , Ku 70 and Ku 86 . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
In addition , flavopiridol treatment ( 300 nM , 1 day ) resulted in decreased levels of Ku 70 and Ku 86 proteins that play a role in DNA repair processes , suggesting that DNA repair processes may have been disrupted by this agent . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
Ku 70 is one component of a protein complex , Ku 70 and Ku 80 , that functions as a heterodimer to bind DNA double strand breaks and activates DNA dependent protein kinase . ^^^ Our previous study with Ku 70 / and Ku 80 / mice , and cell lines has shown that Ku 70 and Ku 80 deficiency compromises the ability of cells to repair DNA double strand breaks , increases radiosensitivity of cells , and enhances radiation induced apoptosis . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
Oxidative stress induces nuclear loss of DNA repair proteins Ku 70 and Ku 80 and apoptosis in pancreatic acinar AR42J cells . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
Several reports indicate that the Ku 70 and Ku 80 subunits of the mammalian DNA dependent protein kinase ( DNA PK ) , a complex involved in NHEJ , appear to be susceptible to a heat induced loss of DNA binding activity , with Ku 80 representing the heat sensitive component . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
DNA PK is a nuclear protein with serine / threonine kinase activity and forms a complex consisting of the DNA PKcs and a heterodimer of Ku 70 and Ku 80 proteins . ^^^ In this study , we evaluated the relationship between expression levels of DNA PKcs , Ku 70 and Ku 80 proteins and radiation sensitivity in oral squamous cell carcinoma ( OSCC ) cell lines and in OSCC patients treated with preoperative radiation therapy . ^^^ Expression of DNA PKcs and Ku 70 after irradiation was increased in the surviving cells of OSCC tissues irradiated preoperatively . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
DNA dependent protein kinase ( DNA PK ) , composed of Ku 70 , Ku 80 , and the catalytic subunit ( DNA PKcs ) , is involved in double strand break ( DSB ) repair by non homologous end joining ( NHEJ ) . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
The decreased kinase activity of DNA PKcs was not due to a change in its amount or the levels of Ku 70 and Ku 86 , their subcellular distribution , cell cycle progression or caspase mediated degradation of DNA PK . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
These results did not correlate with radiation induced apoptosis but did correlate with functional impairment of DNA repair and suppressed expression of several repair related genes , such as Ku 70 , DNA dependent protein kinase , ataxia telangiectasia mutated , and 10 ray sensitive complementation group 4 . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
Ku 70 and Ku 86 together with DNA PKcs form the DNA dependent protein kinase ( DNA PK ) complex that is involved in DNA double strand break repair by nonhomologous end joining . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
Among the genes in this region , hid plays a central , dosage sensitive role in IR induced apoptosis . p 53 and MNK / Chk2 also regulate DNA repair genes , including two components of the nonhomologous end joining repair pathway , Ku 70 and Ku 80 . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
The main components of the NHEJ system in eukaryotes are the catalytic subunit of DNA protein kinase ( DNA PK ( cs ) ) , which is recruited by DNA Ku protein , a heterodimer of Ku 70 and Ku 80 , as well as XRCC 4 protein and DNA ligase 4 . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
Amino acid sequence analysis demonstrated that three proteins , DNA PK catalytic subunit , Ku 86 , and Ku 70 are components of DNA dependent protein kinase ( DNA PK ) . ^^^ AREB 6 like proteins specifically bind to the E box , whereas Ku 86 binds an adjacent site and recruits DNA PK catalytic subunit and Ku 70 proteins . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
Ku 70 and Ku 80 proteins are responsible for the repair of DNA double strand breaks and function as a regulatory subunit of the DNA dependent protein kinase . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
The Ku 70 DNA end joining protein has recently been shown to suppress apoptosis by sequestering Bax from mitochondria . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
During DNA damage , DNA repair proteins Ku 70 and Ku 80 prevent cell death , but severe DNA damage beyond the repair capacity of the DNA repair proteins triggers necrosis or apoptosis . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
The Ku heterodimer , which consists of Ku 70 and Ku 86 subunits , is a major sensor of DNA breaks . ^^^ The resulting Ku 86 variant exists exclusively in complexes with Ku 70 , which possess strong affinity to DNA double strand termini . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
This signal is produced within the damaged cell by the kinase function of the Ku 70 , Ku 80 , and DNA dependent protein kinase complex and is conveyed to the recipient cell by direct cell to cell communication through gap junctions . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
These genes included G22P1 ( Ku 70 ) and XRCC 5 ( Ku 80 ) genes known as important members of the nonhomologous end joining ( NHEJ ) pathway of DNA double strand break ( DSB ) repair . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
The Ku protein is a complex of two subunits , Ku 70 and Ku 80 , and it plays a role in multiple nuclear processes , e . g . , nonhomologous DNA end joining ( NHEJ ) , chromosome maintenance , and transcription regulation . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
Here we describe two PCR based methods to monitor telomere length and examine the fate of dysfunctional telomeres in Arabidopsis lacking the catalytic subunit of telomerase ( TERT ) and the DNA repair proteins Ku 70 and Mre 11 . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
Ku 70 , Ku 80 , DNA PKcs ( the catalytic subunit of DNA dependent protein kinase ) , Xrcc 4 and Artemis all participate in DSB repair through nonhomologous end joining ( NHEJ ) . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
The levels of ku 70 , ku 80 , DNA PKcs , phosphorylated ku 80 as well as the DNA binding activity of the ku70 / ku 80 heterodimer were determined in the cytoplasmic and nuclear extracts obtained , before and after irradiation , from young and elderly subjects . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
RESULTS : Using both supershift and Western analysis , the following proteins were identified in the DNA end binding complex : Ku 70 , Ku 80 , DNA dependent protein kinase catalytic subunit , DNA ligase 4 , 10 ray cross complementing protein 4 , meiotic recombination protein 11 ( Mre 11 ) , Werner ' s syndrome protein , Bloom ' s syndrome protein , p 53 , poly ( ADP ribose ) polymerase , replication protein A ( RPA ) 14 , and RPA 32 , ataxia telangiectasia mutant , c Abl , Rad 50 , Nijmegen breakage syndrome protein 1 ( NBS 1 ) , and DNA ligase 3 were not detected in the binding complex by any assay . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
Unexpectedly , however , we found that nonhomologous end joining ( NHEJ ) , another DSB repair pathway , has no positive role in the relevant repair ; notably , DT 40 cell mutants lacking either of the NHEJ factors ( namely , Ku 70 , DNA dependent protein kinase catalytic subunit , and DNA ligase 4 ) were resistant to killing by CPT . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
Several nuclear and cytosolic proteins showing at least partial binding specificity for RPT 1 were isolated from U 937 and A 549 cells by pull down assays , including Ku 70 ( DNA dependent protein kinase subunit ) and poly ( ADP ribose ) polymerase 1 . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
Western blot analysis indicated that the expression of NHEJ pathway components Ku 70 , XRCC 4 , and DNA ligase 4 was downregulated in NSCLC cells A 549 with Ad mda 7 treatment . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
Agnoprotein expression correlated with impaired double strand break repair activity in cellular extracts and reduced expression of the Ku 70 and Ku 80 DNA repair proteins . ^^^ Since Ku 70 is a subunit of the DNA dependent protein kinase that is responsible both for double strand break repair and for signaling damage induced cell cycle arrest , the modulation of Ku 70 and / or Ku 80 by agnoprotein may represent an important event in the polyomavirus life cycle and in cell transformation . . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
We identified and disrupted Neurospora crassa genes homologous to human KU 70 and KU 80 , which encode proteins that function in nonhomologous end joining of double stranded DNA breaks . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
Oxidative damage and defective DNA repair is linked to apoptosis of migrating neurons and progenitors during cerebral cortex development in Ku 70 deficient mice . ^^^ We examined cortical development in mice deficient for the DNA end joining protein , Ku 70 . ^^^ At gestational day 14 . 5 , corresponding to the peak of neurogenesis , the Ku 70 ( / ) embryonic cerebral cortex displayed 25 to 30 fold more cell death than heterozygous littermates , as judged by DNA breaks , pyknosis and active caspase 3 . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
Ku 70 has activities to repair DNA damage in the nucleus and to suppress apoptosis by inhibiting Bax in the cytosol . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
Intra and inter pathway interactions were quantified by co localisation , FRET imaging and by co immunoprecipitation ( Co IP ) of XRCC 4 , DNA PK and Ku 70 as representatives of NHEJ , Rad 51 and Rad 52 for HRR and gamma H2AX , Mre 11 and Rad 50 as representatives of both pathways . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
To test this possibility , we examined cells of the chicken B lymphocyte cell line DT 40 and its derivatives lacking NHEJ and / or HR : KU 70 ( / ) , DNA PKcs ( / / ) , RAD 54 ( / ) and KU 70 ( / ) / RAD54 ( / ) . ^^^ However , in NHEJ defective KU 70 ( / ) and DNA PKcs ( / / ) , consisting of two subpopulations with distinct radiosensitivity , the radiosensitive subpopulation , which is considered to be cells in G ( 1 ) and early S , was not sensitized . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
In addition , paraffin embedded tissue sections were assessed for proliferation ( PCNA ) , DNA repair ( Ku 70 and gamma H2AX ) , and apoptosis ( TUNEL ) by immunofluorescent staining ( wild type vs . heterozygous only ) at various times after 5 Gy single dose . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
This band was also completely supershifted by the addition of antibodies to ATM , Ku 70 , DNA ligase 3 , Rpa 32 , Rpa 14 , DNA ligase 4 , XRCC 4 , WRN , BLM , RAD 51 and p 53 . ^^^ The density of one of the DNA EBCs predicted the radiosensitization caused by BRCA haploinsufficiency , and this band contains Ku 70 , ATM , DNA ligase 3 , Rpa 32 , Rpa 14 , DNA ligase 4 , XRCC 4 , WRN , BLM , RAD 51 and p 53 but not BRCA 1 or 2 . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
To unravel a potential role of cyclin A 1 in DNA repair , we performed a yeast triple hybrid screen and identified the Ku 70 DNA repair protein as a binding partner and substrate of the cyclin A 1 CDK2 complex . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
Junctions were formed by the non random deletion of terminal nucleotides and showed a preference for annealing at a microhomology of 8 nt buried within the DNA substrate ; this process was not dependent on functional Ku 70 , DNA PK or XRCC 4 . ^^^ Junctions were repaired in the same manner in MO59K extracts in which accurate NHEJ was inactivated by inhibition of Ku 70 or DNA PK ( cs ) . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
In addition , global methylation is suppressed and key DNA repair proteins are induced in tissues from chronically irradiated animals ( specifically AP endonuclease , polymerase beta , and Ku 70 ) . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
The budding yeast Saccharomyces cerevisiae repairs DSB primarily by homologous recombination ( HR ) , despite the presence of the KU 70 , KU 80 , DNA ligase 4 and XRCC 4 homologues , essential factors of the mammalian non homologous end joining ( NHEJ ) machinery . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
DNA dependent protein kinase ( DNA PK ) containing the regulatory subunits Ku 70 and Ku 80 plays a prominent role in the repair of double stranded DNA breaks by a nonhomologous end joining pathway maintaining genomic stability . ^^^ In an attempt to elucidate the significance of the DNA PK complex for human urothelial carcinogenesis , the expression of Ku 70 and Ku 80 was studied in 71 transitional cell carcinomas ( TCC ) of the urinary bladder of various grades and stages , and in relation to lifestyle and occupational bladder cancer risk factors . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
The core components of mammalian NHEJ are the catalytic subunit of DNA protein kinase ( DNA PK ( cs ) ) , Ku subunits Ku 70 and Ku 80 , Artemis , XRCC 4 and DNA ligase 4 . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
Actinomycin D induces histone gamma H2AX foci and complex formation of gamma H2AX with Ku 70 and nuclear DNA helicase 2 . ^^^ In untreated cells , gamma H2AX co immunoprecipitated with Ku 70 , a subunit of DNA dependent protein kinase , as well as with nuclear DNA helicase 2 ( NDH 2 ) , a DEXH family helicase also known as RNA helicase A or DHX 9 . ^^^ DNA degradation diminished the amount of Ku 70 associated with gamma H2AX but not that of NDH 2 . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
The TRF 2 DSB response requires the TRF 2 basic domain but not its Myb domain and occurs in the absence of functional ATM and DNA PK protein kinases , MRE11 / Rad50 / NBS1 complex and Ku 70 , WRN and BLM repair proteins . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
Here we show that a decrease in Ku 70 or Ku 80 gene dosage causes a sharp increase in the frequency of HeT A and TART attachments to a broken chromosome end and in terminal DNA elongation by gene conversion . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
Regulation of homologous integration in yeast by the DNA repair proteins Ku 70 and RecQ . ^^^ We present evidence for a genetic interaction between SGS 1 and YKU 70 , which encodes the S . cerevisiae homologue of the human DNA helicase Ku 70 . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
The Ku 70 binding site of Ku 80 is required for the stabilization of Ku 70 in the cytoplasm , for the nuclear translocation of Ku 80 , and for Ku 80 dependent DNA repair . ^^^ It is believed that heterodimerization between Ku 70 and Ku 80 is essential for Ku dependent DNA repair , although its role is poorly understood . ^^^ In addition , our results suggest that the nuclear translocation of Ku 80 through the Ku 70 binding site as well as through the NLS of Ku 80 play , at least in part , a role in Ku 80 dependent DNA repair . ^^^ Furthermore , our results suggest the possibility that Ku 80 has a DNA DSB repair function independent of Ku 70 in the nuclei , in addition to that dependent on Ku70 . . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
OBJECTIVES : Ku , a heterodimer of KU 70 and Ku 80 that binds to double strand DNA breaks ( DSBs ) and activates the catalytic subunit ( DNA PKcs ) when DNA is bound , is essential in DSB repair and 5 ( D ) J recombination . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
We previously identified four DNA PK phosphorylation sites on the Ku70 / 80 heterodimer : serine 6 of Ku 70 , serine 577 and 580 and threonine 715 of Ku 80 . ^^^ These results suggest that the phosphorylation of Ku 70 and Ku 80 is regulated by a protein phosphatase 2A like protein phosphatase and a staurosporine sensitive protein kinase in vivo , but that DNA PK mediated phosphorylation of Ku is not required for DNA double strand break repair . . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
Twelve loci of selected DNA repair genes were genotyped by matrix assisted laser desorption / ionization time of flight mass spectrometry ( XRCC 2 Arg188His , XRCC 4 921G > T , XRCC 6 1796G > T , LIG 4 1977T / C , RAD 51 135G > C , 172G > T , RAD 52 2259C > T , LIG 1 551A > C , ERCC 1 8092A > C , 354C > T , hMLH 1 93G > A , and Ile219Val ) . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
We chose cells deficient in Ku 70 ( DNA end joining ) , Xrcc 2 ( gene conversion ) , Ercc 1 ( single strand annealing ) and Csb ( transcription coupled repair ) to represent potentially important DNA repair pathways , plus an Xpc deficient line to examine loss of global nucleotide excision repair ( NER ) . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
LOH was recurrently observed for 8 of the 9 studied genes ( KU 70 , KU 80 , XRCC 4 , LIG 4 , Artemis , MRE 11 , RAD 50 , NBS 1 ) but not for DNA PKcs . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
Moreover , NaB significantly reduced the expression of the repair related genes Ku 70 and Ku 86 and DNA dependent protein kinase catalytic subunit in melanoma cells at the protein and mRNA levels . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
The consistent level of Ku 70 protein in all the treated samples , with undetectable level of Rad 51 in the G 0 lymphocytes indicates the involvement of NHEJ pathway in misrejoining of DNA dsbs . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
DAYSLEEPER was isolated as a factor binding to a motif ( Kubox 1 ) present in the upstream region of the Arabidopsis DNA repair gene Ku 70 . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
Ku 70 , DNA PKcs , DNA Ligase 4 , Xrcc 4 ) were observed . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
Of the twelve proteins identified , three appear to be relevant to NASP ' s function : heat shock protein 90 ( HSP 90 ) , DNA activated protein kinase , and ATP dependent DNA helicase 2 ( 70 kDa subunit ) . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
Western blot analysis demonstrated that celecoxib downregulated the expression of Ku 70 protein and inhibited the kinase activity of DNA PKcs , which are involved in the double stranded DNA break repair machinery . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
In addition , we have identified Ku 70 , a DNA binding protein that is required for DNA damage repair , as a new ARF 6 interacting protein and found that it is part of a complex with ARF 6 , especially during mitosis . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
There was no change in the protein levels of DNA PKcs , Ku 70 , or Ku 86 . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
Despite the observed defects in the repair efficiency , no apparent differences in homologous recombination or non homologous end joining effector proteins RAD 51 , KU 70 , KU 86 , or DNA PK ( CS ) were observed . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
Ku , the heterodimer of Ku 70 and Ku 86 , plays crucial roles in non homologous end joining ( NHEJ ) , a major pathway for repairing DNA double strand breaks ( DSBs ) in mammalian cells . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
As expected , 5 ( D ) J recombination was substantially impaired in cells deficient for the NHEJ components Ku 70 , XRCC 4 , and DNA PKcs . ^^^ HDR was increased to the largest extent in Ku 70 / cells , implicating the Ku70 / 80 DNA end binding protein in regulating pathway choice . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
DNA double strand breaks ( dsb ) during 5 ( D ) J recombination of T and B lymphocyte receptor genes are resolved by the non homologous DNA end joining pathway ( NHEJ ) including at least six factors : Ku 70 , Ku 80 , DNA PK ( cs ) , Artemis , Xrcc 4 , and DNA ligase 4 ( Lig 4 ) . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
Ku 70 plays an important role in DNA damage repair and prevention of cell death . ^^^ Ku 70 forms a heterodimer with Ku 80 , which is required for the DNA repair activity of Ku proteins . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
Tumors , with increased DNA binding activity , also showed a statistically significant increase in Ku 70 and Ku 86 nuclear expression , as determined by Western blot and immunohistochemical analyses ( P < 0 . 001 ) . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
Neither Ku 70 nor Ku 80 disrupted strains show hypersensitivity to the DNA damaging agents methylmethane sulfonate ( MMS ) and phleomycin . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
Granzyme A , which causes single stranded DNA damage , targets the double strand break repair protein Ku 70 . ^^^ Ku 70 is cut after Arg ( 301 ) , disrupting Ku complex binding to DNA . ^^^ Cleaving Ku 70 facilitates GzmA mediated cell death , as silencing Ku 70 by RNA interference increases DNA damage and cell death by GzmB cluster deficient cytotoxic T lymphocytes or by GzmA and perforin , whereas Ku 70 overexpression has the opposite effect . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
A significant number of these proteins could be assigned to known DNA DSB response processes , such as sensing DSBs ( Ku 70 ) , DNA repair through effectors ( high mobility group protein 1 ) , or cell cycle arrest at the G ( 2 ) M phase checkpoint ( 14 3 3 zeta ) . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
In the koji molds Aspergillus sojae and Aspergillus oryzae , exogenous DNA is integrated in the genome , in most cases irrespective of the sequence homology , suggesting that DNA integration occurs predominantly through a nonhomologous end joining pathway where two ku genes , namely , ku 70 and ku 80 , play a key role . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
In prostate cancer cells the interaction of C / EBPalpha with Ku 70 , Ku 80 , and poly ( ADP ribose ) polymerase 1 increases sensitivity to DNA damage . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
Ku 70 and Ku 80 proteins take part in the repairing of DNA double strand breaks by their function as a regulatory subunit of the DNA dependent protein kinase . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
This article reports a combined investigation of molecular dynamics ( MD ) simulation and automated docking that evidence the molecular regions of Bax involved in the binding with anti apoptotic exapeptide ( Bip ) designed from Ku 70 , a subunit of the protein complex essential for non homologous DNA repair but that inhibits also the Bax translocation to mitochondria . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
We found that tumour transformed tissue shows positive immunostaining of DNA PKcs , Ku 86 and Ku 70 , while non neoplastic squamous epithelium and tumour free cervix glands show negative immunoreactivity . ^^^ Expression of DNA PKcs positively correlated with both Ku 86 and Ku 70 , and a statistically significant correlation between the Ku subunits was also found . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
Altered expression of DNA double strand repair genes Ku 70 and Ku 80 in carcinomas of the oral cavity . ^^^ The main aim of this study was to analyse the expression of the heterodimers Ku 70 and Ku 80 , building regulatory subunits of the DNA dependent protein kinase in 40 oral carcinomas . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
Ku 70 Ku80 heterodimers promote the non homologous end joining ( NHEJ ) of DNA breaks and , as shown here , the fusion of dysfunctional telomeres . ^^^ Telomeres of Ku 70 ( / ) cells had a normal DNA structure and did not activate a DNA damage signal . ^^^
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
NA
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
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Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
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Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
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Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
NA
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
NA
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
NA
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
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Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
NA
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
NA
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
NA
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
NA
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
NA
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
NA
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
NA
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
NA
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
NA
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
NA
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
NA
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
NA
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
NA
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
NA
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
NA
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
NA
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
NA
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
NA
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
NA
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
NA
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
NA
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
NA
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
NA
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
NA
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
NA
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
NA
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
NA
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
NA
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
NA
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
NA
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
NA
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
NA
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
NA
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
NA
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
NA
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
NA
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
NA
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
NA
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
NA
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
NA
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
NA
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
NA
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
NA
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
NA
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
NA
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
NA
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
NA
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
NA
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
NA
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
NA
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
NA
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
NA
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
NA
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
NA
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
NA
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
NA
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
NA
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
NA
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
NA
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
NA
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
NA
Interacting proteins: P27695 and P12956	Pubmed	SVM Score :0.0
NA