Pubmed abstracts for Protein-Protein Interaction search result :

Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.77943661
Defining the minimal domain of Ku 80 for interaction with Ku 70 . 0.77943661^^^ The minimal functional interaction domain of Ku 80 that interacted with Ku 70 was defined . 0.77611357^^^ Ku 80 was restricted to interacting with just one Ku 70 molecule at a time . 0.6025^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.71725459
Furthermore , we found that the Ku 80 protein was transported to the nucleus without heterodimerization with Ku 70 . 0.71725459^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.65325201
Using the site directed mutagenesis technique , we demonstrate here that Ku 70 can translocate to the nucleus without heterodimerization with Ku 80 . 0.65325201^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.54675646
Ku 70 forms a heterodimer with Ku 80 , which is required for the DNA repair activity of Ku proteins . 0.54675646^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
The CTCBF contains two subunits , CTC 75 and CTC 85 , with molecular weights of 75 and 85 kDa , respectively . ^^^ The results presented support the idea that the subunits CTC 75 and CTC 85 ( identical or homologous to p 70 and p 80 of the Ku antigen ) are integral parts of CTCBF , and give a first indication of the importance of TBP in the formation of CTCBF . . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
The DNA dependent protein kinase ( DNA PK ) consists of three polypeptide components : Ku 70 , Ku 80 , and an approximately 350 kDa catalytic subunit ( p 350 ) . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
Ku 80 protein forms heterodimer with the Ku 70 subunit to form a complex that possesses a DNA end binding activity . ^^^ However , introduction of human Ku 80 gene into the mutant lead to increased expression of Ku 70 protein and restored Ku 70 binding to DNA ends , suggesting that mutation of the Ku 80 gene affected the formation of Ku70 / Ku80 dimers and the stability of the Ku 70 protein . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
DNA dependent protein kinase ( DNA PK ) consists of three polypeptide subunits : Ku 70 , Ku 80 , and the DNA PK catalytic subunit ( DNA PKcs ) . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
To study the physiological role of CHBF in the regulation of heat shock response , we purified CHBF to apparent homogeneity and showed it to be identical to the Ku autoantigen , a heterodimer consisting of 70 kDa ( Ku 70 ) and 86 kDa ( Ku 80 ) polypeptides . ^^^ We show that expression of the human Ku 70 and Ku 80 subunits jointly or of the Ku 70 subunit alone specifically inhibits heat induced hsp 70 expression . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
Ku , a heterodimer of polypeptides of approximately 70 kDa and 80 kDa ( Ku 70 and Ku 80 , respectively ) , binds avidly to DNA double strand breaks ( DSBs ) . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
We have analyzed expression of Ku 70 , Ku 80 and DNA PKcs upon induction of differentiation in preB cells derived from wild type , severe combined immunodeficiency ( SCID ) and Rag 2 / mice . ^^^ Protein levels of Ku 80 and Ku 70 moderately decrease after induction in all three cell types . ^^^ In mouse preB cells , Ku 70 and Ku 80 are present in the nuclei and cytoplasm before and after onset of differentiation . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
While the formation of the Ku 70 and Ku 80 complex is important for repair of DNA double strand breaks our data suggest that the 70 kDa subunit of Ku plays a role in regulating hsp 70 expression . . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
Ku is a heterodimer of polypeptides of approximately 70 and 80 kDa ( Ku 70 and Ku 80 , respectively ) that binds to DNA ends . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
DNA dependent protein kinase ( DNA PK ) consists of a 460 kDa subunit that contains the catalytic domain ( DNA PKcs ) complexed with two polypeptides of 70 kDa and 80 kDa ( Ku 70 and Ku 80 ) which comprise the Ku autoantigen . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
All mutants examined have low or undetectable levels of Ku 70 and Ku 80 protein , end binding , and DNA PK activities . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
The DNA dependent protein kinase ( DNA PK ) is a trimeric enzyme consisting of a 460 kDa catalytic subunit ( DNA PKcs ) and a heterodimeric regulatory complex called Ku , which is comprised of 70 ( Ku 70 ) and 86 ( Ku 80 ) kDa subunits . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
The Ku 70 protein is an additional component of DNA PK ; Ku 70 forms a heterodimer with Ku 80 to generate the DNA end binding component of the enzyme . ^^^ We find that the Ku 70 ( / ) cells produce no detectable Ku 70 and very little Ku 80 , suggesting a direct interrelationship between their levels . ^^^ Potential differences between the Ku 70 ( / ) and Ku 80 ( / ) 5 ( D ) J recombination defects are discussed . . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
Both Ku 80 and Ku 70 polypeptides were present in high concentrations in human placenta and HeLa cells . ^^^ While cotransfection of pcDNA Ku 80 with pHGR ( 892 to 878 ) CAT potentiated the expression of CAT , introduction of pcDNA Ku 70 did not affect the expression of CAT in transfected MCF 7 cells . ^^^ Supershift assays with monoclonal antibodies Ab 111 ( Ku 80 ) or Ab N3H10 ( Ku 70 ) showed a direct interaction of GREF 1 and GREF 2 heterodimers with the palindrome . ^^^ Partial peptide fingerprinting of GREF 1 and GREF 2 using alpha chymotrypsin and immunoblotting with Ab 111 and Ab N3H10 confirmed their identities as Ku 80 and Ku 70 , respectively . . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
Normal protein and transcription levels of Ku 70 and Ku 80 , as well as DNA PK activity , were found in six different AT cell lines , 1 4 h following exposure to ionizing radiation , timepoints where reduced and delayed transcriptional induction of WAF1 / CIP1 ( p 21 ) was observed . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
Ku is a complex of two proteins , Ku 70 and Ku 80 , and functions as a heterodimer to bind DNA double strand breaks ( DSB ) and activate DNA dependent protein kinase . ^^^ Like Ku 80 ( / ) mice , Ku 70 ( / ) mice showed a profound deficiency in DNA DSB repair and were proportional dwarfs . ^^^ Surprisingly , in contrast to Ku 80 ( / ) mice in which both T and B lymphocyte development were arrested at an early stage , lack of Ku 70 was compatible with T cell receptor gene recombination and the development of mature CD4+CD8 and CD 4 CD8+ T cells . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
The DNA PKcs gene encodes the 465 kDa catalytic subunit of DNA dependent protein kinase ( DNA PK ) , which associates with heterodimeric autoantigens Ku 70 and Ku 80 and exhibits protein kinase activity depending on DNA double strand breaks . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
Double strand break repair by Ku 70 requires heterodimerization with Ku 80 and DNA binding functions . ^^^ Heterodimers of the 70 and 80 kDa Ku autoantigens ( Ku 70 and Ku 80 ) activate the DNA dependent protein kinase ( DNA PK ) . ^^^ Mutations in any of the three subunits of this protein kinase ( Ku 70 , Ku 80 and DNA PKcs ) lead to sensitivity to ionizing radiation ( IR ) and to DNA double strand breaks , and 5 ( D ) J recombination product formation defects . ^^^ The propensity for Ku 70 to associate with Ku 80 and to bind DNA correlates with the ability to activate DNA PK , although two mutants showed that the roles of Ku 70 in DNA PK activation and IR repair are separate . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
Ku 70 , Ku 80 , and DNA PKcs are subunits of the DNA dependent protein kinase ( DNA PK ) , an enzyme implicated in DNA double stranded break repair and 5 ( D ) J recombination . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
We demonstrate here that the products of the catalytic subunit of DNA dependent protein kinase ( DNA PKcs ) and its regulatory subunits ( Ku 70 and Ku 80 ) are present at normal levels and possess functional activity . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
A central region of Ku 80 mediates interaction with Ku 70 in vivo . ^^^ Ku , the DNA binding component of DNA dependent protein kinase ( DNA PK ) , is a heterodimer composed of 70 and 86 kDa subunits , known as Ku 70 and Ku 80 respectively . ^^^ A previous study has suggested that the C terminus of Ku 80 is required for interaction with Ku 70 . ^^^ Our two hybrid study suggests that the central region of Ku 80 , not its C terminus , is capable of mediating interaction with Ku 70 . ^^^ To determine if this region mediates interaction with Ku 70 in mammalian cells we transfected xrs 6 cells , which lack endogenous Ku 80 , with epitope tagged Ku 80 deletions carrying a nuclear localization signal . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
Ku 70 and Ku 80 form a DNA end binding complex required for DNA double strand break repair and 5 ( D ) J recombination . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
Ku , consisting of an Mr 70 , 000 ( Ku 70 ) and an Mr 86 , 000 ( Ku 80 ) subunit , is a heterodimeric nuclear protein and is the DNA binding regulatory component of the mammalian DNA dependent protein kinase DNA PK . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
The DNA dependent protein kinase is a mammalian protein complex composed of Ku 70 , Ku 80 , and DNA PKcs subunits that has been implicated in DNA double strand break repair and 5 ( D ) J recombination . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
The DNA dependent protein kinase ( DNA PK ) consists of Ku 70 , Ku 80 , and a large catalytic subunit , DNA PKcs . ^^^ Targeted inactivation of the Ku 70 or Ku 80 genes results in elevated ionizing radiation ( IR ) sensitivity and inability to perform both 5 ( D ) J coding end and signal ( RS ) end joining in cells , with severe growth retardation plus immunodeficiency in mice . ^^^ We conclude that Ku 70 and Ku 80 may have functions in 5 ( D ) J recombination and DNA repair that are independent of DNA PKcs . . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
From the analysis of defective rodent mutants , four proteins ( Ku 70 , Ku 80 , DNA PKcs and Xrcc 4 ) that function in this process in mammalian cells have been identified . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
All of these phenotypic characteristics , except embryonic lethality , resemble those associated with Ku 70 and Ku 80 deficiencies , indicating that they may result from an impaired end joining process that involves both Ku subunits and ligase 4 . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
The Ku protein is a complex of Ku 70 and Ku 80 subunits and is capable of binding promoters in a sequence specific manner , although it remains unclear whether Ku is involved in transcriptional regulation . ^^^ Our results indicate that heterodimers of Ku 70 and Ku 80 are localized in the nucleus , and that the stretches from amino acid ( aa ) 378 to 482 of Ku 70 and from aa 374 to 502 of Ku 80 are necessary for heterodimerization . ^^^ To determine whether Ku contains a potential transcriptional activation domain , we examined N and C terminal deletion mutants of Ku 70 and Ku 80 for their ability to activate transcription in the GAL 4 based one hybrid system . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
NF 90 and NF 45 associate with the DNA dependent protein kinase catalytic subunit and the DNA targeting subunits Ku 80 and Ku 70 ( N . ^^^ The purine box transcriptional regulator in 16HBE cells likely comprises NF 45 , NF 90 , Ku 80 , Ku 70 , and the DNA dependent protein kinase catalytic subunit . . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
Interestingly , either Ku 70 or Ku 80 deficient cell lines , but not mutant cell lines of the DNA PK catalytic sub unit ( DNA PKcs ) , were found to be hypersensitive to the effects of etoposide VP 16 . ^^^ Ku 70 and Ku 80 deficient cells can be complemented to an etoposide resistant phenotype by introducing wildtype Ku 70 or Ku 80 cDNAs . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
The other components of the DNA PK Ku autoantigen complex , Ku 70 and Ku 80 , were 3 fold increased and unchanged , respectively . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
While Ku 70 cross linked to the upper strand of NRE 1 when Ku was bound to double stranded and single stranded DNAs , the Ku 80 subunit only cross linked to single stranded NRE 1 . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
Molecular cloning and sequencing of cDNAs encoding homologues of human Ku 70 and Ku 80 autoantigen from Xenopus and their expression in various Xenopus tissues . ^^^ We isolated cDNA clones encoding Ku 70 and Ku 80 homologues of Xenopus laevis from a cDNA library prepared from Xenopus oocytes . ^^^ The nucleotide sequences of these Ku 70 and Ku 80 homologues have coding sequences of 1833 bp and a 611 aa protein , and 2178 bp and a 726 aa protein , respectively . ^^^ The amino acid sequences deduced from the open reading frame of the Ku 70 and Ku 80 cDNA clones were highly homologous to those from Ku genes previously isolated , such as human ( ca . 65 % and ca . 62 % identity , respectively ) and mouse ( ca . 65 % and ca . 60 % ) , and show a certain degree of homology to Drosophila ( ca . 27 % with Ku 70 ) , Caenorhabditis elegans ( ca . 20 % with Ku 80 ) and Saccharomyces cerevisiae ( ca . 23 % and ca . 19 % ) . ^^^ Our detailed comparison of the predicted amino acid sequences among these species revealed the highly conserved octa peptide LPFXXDIR common to both Xenopus Ku 70 and Ku 80 homologues in the region showing the high homology throughout the species tested . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
Aside from DNA PK , XRCC 4 and DNA ligase 4 , other proteins which play a role ( s ) in this repair pathway remain unknown ; DNA PK contains a catalytic subunit ( DNA PKcs ) and a DNA binding subunit ( Ku 70 and Ku 80 ) . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
They reacted with human Ku 70 and Ku 80 , respectively , by immunoblotting . ^^^ When HeLa cell nuclear proteins were fractionated and applied to immunoblotting , both Ku 70 and Ku 80 were detected in the nuclear matrix as well as the soluble nuclear protein fractions . ^^^ By confocal scanning microscopy , the immunoreactivity of Ku 70 and Ku 80 was localized to distinct nucleoplasmic fibrillar network and fine granules in the interphase cell nuclei . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
The DNA dependent protein kinase ( DNA PK ) consists of a heterodimer DNA binding complex , Ku 70 and Ku 80 , and a large catalytic subunit , DNA PKcs . ^^^ In contrast to the Ku 70 / and Ku 80 / phenotype , DNA PKcs null mice are blocked for 5 ( D ) J coding but not for signal end joint formation . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
Ku antigen is composed of 70 and 82 kDa subunits ( Ku 70 and Ku 80 , respectively ) that together bind with high affinity to ends of double stranded DNA and other DNA structures in vitro . ^^^ We find that Ku 70 is phosphorylated at a single serine residue , serine 6 , located in the putative transcriptional activation domain , and Ku 80 is phosphorylated at serines 577 and 580 and at threonine 715 . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
The expression of the Ku 70 and Ku 80 genes as well as the activity of the DNA dependent protein kinase ( DNA PK ) were studied in 11 normal human fibroblast lines . ^^^ We found that the mRNA signal intensities of both Ku 70 and Ku 80 genes were fairly similar for the 11 cell lines investigated . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
This protein contributes to the repair of DNA double strand breaks ( DSBs ) by assembling broken ends of DNA molecules in combination with the DNA binding factors Ku 70 and Ku 80 . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
Ku comprises two tightly associated subunits of approximately 69 and approximately 83 kDa , which are termed Ku 70 and Ku 80 ( or Ku 86 ) , respectively . ^^^ We have identified a region in Ku 70 ( amino acid residues 449 578 ) and a region in Ku 80 ( residues 439 592 ) that participate in Ku subunit interaction . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
Using different antibodies to immunoprecipitate the individual components of the DNA PK complex ( DNA PKcs , Ku 70 , and Ku 80 ) , it was shown that APA SG caused a destabilization of the trimeric holoenzyme complex by dissociating the catalytic subunit from the Ku heterodimer . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
The effect of an overexpression of human Ku70 / 80 was studied using cells of the rat cell lines Rat 1 and R 7080 , the latter being transfected with the human cDNAs for Ku 70 and Ku 80 . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
Trans acting factors Ku 70 , Ku 80 and possibly SWAP 70 have been identified for the temporally related but not mechanistically related immunoglobulin heavy chain class switch . . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
DNA PKcs , Ku 80 , and Ku 70 mRNAs were expressed in all normal tissues with relatively little variation in levels . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
Targeted disruption of the genes encoding Ku 70 and Ku 80 has identified significant differences between Ku deficient mice and DNA PKcs deficient mice . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
The heterodimeric Ku protein , composed of 86 kDa ( Ku 80 ) and 70 kDa ( Ku 70 ) subunits , is the DNA targeting component of DNA dependent protein kinase ( DNA PK ) , which plays a critical role in mammalian DNA double strand breaks repair . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
The complex has a strongly preferred orientation , with Ku 70 DNA backbone contacts located proximal and Ku 80 DNA backbone contacts located distal to the free end . ^^^ Results are consistent with a model where the Ku 70 and Ku 80 subunits contact the major and minor grooves of DNA , respectively . . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
Ku protein is a complex of two subunits , Ku 70 and Ku 80 . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
We have recently defined a central region within Ku 80 that is required for heterodimerization with Ku 70 . ^^^ Interaction with Ku 70 and DNA end binding are important for Ku 80 function in vivo , since Ku 80 mutants lacking DNA end binding activity were unable to restore radiation resistance in Ku 80 deficient fibroblast cell lines . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
Ku null mutant cell lines Ku 70 / and Ku 80 / were highly sensitive to anticancer drugs , compared with their wild type cells . ^^^ Ku deficient cells were more sensitive to bleomycin induced DNA fragmentation and exhibited a higher level of c jun NH 2 kinase / stress activated PK activity than wild type cells , whereas R 7080 6 cells overexpressing both human Ku 70 and Ku 80 were resistant to bleomycin induced apoptosis and exhibited a lower level of c jun NH 2 kinase / stress activated PK activity . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
The highly expressed Ku heterodimer , composed of 70 and 80 K ( d ) subunits ( Ku 70 and Ku 80 ) , is the high affinity DNA binding component of the DNA dependent protein kinase . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
The Ku protein is a complex of two subunits , Ku 70 and Ku 80 . ^^^ Using immunofluorescence analysis and confocal laser scanning microscopy , we detected Ku 70 and Ku 80 in the nuclei in interphase cells . ^^^ Using indirect immunoprecipitation , we found that throughout the cell cycle , Ku 70 and Ku 80 were present as heterodimers , some in complex with DNA PKcs . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
Virally transformed cells derived from Ku 70 and Ku 80 knockout mice also displayed end to end fusions . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
Ku 70 and Ku 80 have been shown to form a heterodimeric complex that can bind tightly to free DNA ends and activate the protein kinase DNA PKcs . ^^^ We have found that irradiation results in an up regulation of cellular levels of Ku 70 , but not Ku 80 , and that this enhanced level of Ku 70 accumulates within the nucleus . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
Any defect in one of the known components of the DNA repair / V ( D ) J recombination machinery ( Ku 70 , Ku 80 , DNA PKcs , XRCC 4 and DNA ligase 4 ) leads to abortion of the 5 ( D ) J rearrangement process , early block in both T and B cell maturation , and ultimately to severe combined immune deficiency ( SCID ) in several animal models . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
Using Western blots we measured , in non irradiated cells , the basal expression levels of ATM , Rad 1 and Hus 1 , involved in the control of cellular DNA damage checkpoints , together with DNA PKcs , Ku 70 , Ku 80 ; XRCC 4 , ligaseIV and Rad 51 , involved in radiation induced DSB repair . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
Mammalian nonhomologous DNA end joining employs Ku 70 , Ku 80 , DNA dependent protein kinase catalytic subunit ( DNA PKcs ) , XRCC 4 , and DNA ligase 4 ( Lig 4 ) . ^^^ Herein , we show that Ku 70 and Ku 80 deficiency but not DNA PKcs deficiency results in dramatically increased death of developing embryonic neurons in mice . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
Its regulatory subunit Ku ( dimer of the Ku 70 and Ku 80 protein ) binds to DNA and recruits the kinase catalytic sub unit , DNA PKcs . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
Ku is a heterodimeric protein composed of approximately 70 and approximately 80 kDa subunits ( Ku 70 and Ku 80 ) originally identified as an autoantigen recognized by the sera of patients with autoimmune diseases . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
This inhibition is associated with decreased TNFR associated factor 2 binding to CD 40 , decreased NF kappaB binding to the CD 40 responsive element of the Cgamma 3 promoter , decreased Igamma 3 Cgamma3 germline gene transcription , and decreased expression of Ku 70 , Ku 80 , DNA protein kinase , switch associated protein 70 , and Msh 2 CSR associated transcripts . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
DNA PK is a nuclear serine / threonine kinase that comprises three components : a catlytic subunit ( DNA PKCS ) and two regulatory subunits , DNA binding proteins , Ku 80 and Ku 70 . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
Peptide sequence analysis indicates that the two proteins are identical to Ku 70 and Ku 80 , a heterodimer involved in double strand DNA break repair by non homologous DNA end joining . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
Efficient NHEJ of sticky ends requires the Ku 70 and Ku 80 proteins and the silencing genes SIR 2 , SIR 3 and SIR 4 . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
We identified the binding activity as Ku antigen in its two components : Ku 80 and Ku 70 . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
We have utilized the yeast two hybrid system to identify Ku 70 interacting proteins other than Ku 80 . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
Effects of HDF 1 ( Ku 70 ) and HDF 2 ( Ku 80 ) on spontaneous and DNA damage induced intrachromosomal recombination in Saccharomyces cerevisiae . ^^^ HDF 1 and HDF 2 , which have been identified in Saccharomyces cerevisiae as homologues of the Ku 70 and Ku 80 proteins of mammals , reduce radiosensitivity only when homologous recombination repair is impaired and , therefore , affect DSB repair via nonhomologous recombination . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
Using immunohistochemistry of formalin fixed sections of tumour biopsies , antibodies to Ku 70 and Ku 80 stained identical regions of tumour and there was a high degree of correlation between the mean number of cells stained positive for the two components in 77 tumours ( r = 0 . 82 , P < 0 . 001 ) . ^^^ In 53 tumours there was a borderline significant correlation between measurements of tumour radiosensitivity ( surviving fraction at 2 gray : SF 2 ) and Ku 70 expression ( r = 0 . 26 , P = 0 . 057 ) and no correlation for Ku 80 ( r = 0 . 18 , P = 0 . 19 ) . ^^^ However , all tumours with a low number of Ku 70 or Ku 80 positive cells were radiosensitive . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
Nuclear localization of Ku antigen is promoted independently by basic motifs in the Ku 70 and Ku 80 subunits . ^^^ A bipartite basic nuclear localization signal between amino acids 539 556 of Ku 70 was observed to be required for nuclear import of full length Ku 70 monomer , while a short Ku 80 motif of four amino acids from 565 568 containing three lysines was required for the nuclear import of full length Ku 80 . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
While the Ku complex , comprised of Ku 70 and Ku 80 , is primarily involved in the repair of DNA double strand breaks , it is also believed to participate in additional cellular processes . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
Hypertonic treatment inhibits radiation induced nuclear translocation of the Ku proteins G22p1 ( Ku 70 ) and Xrcc 5 ( Ku 80 ) in rat fibroblasts . ^^^ The effects of 10 irradiation and hypertonic treatment with 0 . 5 M NaCl on the subcellular localization of the Ku proteins G22p1 ( also known as Ku 70 ) and Xrcc 5 ( also known as Ku 80 ) in rat fibroblasts with normal radiosensitivity were examined using confocal laser microscopy and immunoblotting . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
Additionally , we observed an increased interaction of PCNA with the Ku 70 and Ku 80 heterodimer after DNA damage , suggestive of a role for PCNA in the non homologous end joining repair pathway of DNA strand breaks . . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
However , although Lig 4 deficiency causes late embryonic lethality , deficiency in DNA PK subunits ( Ku 70 , Ku 80 , and DNA PKcs ) does not . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
Five components function in this pathway , of which three ( Ku 70 , Ku 80 , and the DNA dependent protein kinase catalytic subunit [ DNA PKcs ] ) constitute a complex termed DNA dependent protein kinase ( DNA PK ) . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
The complex includes the ku heterodimer , which comprises ku 70 and ku 80 subunits , that binds DNA termini of breaks without sequence specificity , and the catalytic subunit DNA PKCS : The activation of the DNA PK complex was studied in 10 irradiated peripheral blood mononuclear cells ( PBMC ) from subjects of different ages . ^^^ Radiation induced changes in the DNA binding activity of the ku heterodimer , and in the concentrations of ku 70 , ku 80 , DNA PKcs and phosphorylated ku 80 were determined in nuclear and cytoplasmic extracts . ^^^ The radiation induced activation of the DNA PK complex may result from the increased concentrations of ku 80 and DNA PKcs in the cytoplasm of PBMC from young , but not from elderly subjects , leading to a higher concentration of phosphorylated ku 80 which readily migrates to the nucleus where , after dimerization with ku 70 , binds to DNA breaks . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
Ku , a heterodimer of Ku 70 and Ku 80 , plays a key role in multiple nuclear processes , e . g . ^^^ Some lines of evidence suggest that heterodimerization is required for the stabilization of Ku 70 and Ku 80 . ^^^ When transfected into Ku deficient xrs 6 cells , exogenous Ku 70 and Ku 80 tagged with green fluorescent protein accumulated into the nucleus , whereas each nuclear localization signal ( NLS ) dysfunctional mutant was undetectable in the nucleus , supporting the idea that each Ku can translocate to the nucleus through its own NLS . ^^^ In Ku expressing HeLa cells , each NLS dysfunctional mutant , as well as wild type Ku 70 and Ku 80 , was still detectable in the nucleus , whereas the double mutant of each Ku subunit with decreased functions of both nuclear targeting and dimerization was undetectable in the nucleus . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
The proteins Ku 70 ( 69 . 8 kDa ) and Ku 80 ( 82 . 7 kDa ) form a heterodimeric complex that is an essential component of the nonhomologous end joining DNA double strand break repair pathway in mammalian cells . ^^^ We have solved the solution structure of the Ku 80 independent DNA binding domain of Ku 70 encompassing residues 536 609 using nuclear magnetic resonance spectroscopy . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
Normal levels of expression of Ku 70 and Ku 80 protein were found by Western blot analysis . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
Expression and chromosome location of hamster Ku 70 and Ku 80 . ^^^ To understand the fundamental characteristics of Ku proteins , we examined the electrophoretic mobility and expression of hamster Ku 70 and Ku 80 and determined the chromosome locations of their genes . ^^^ The Ku 70 gene was localized to Syrian hamster chromosome 4qa4 . 1 > qa4 . 2 and Chinese hamster chromosome 2p3 . 1 , and the Ku 80 gene was localized to Syrian hamster chromosome 4qb5 > qb6 . 1 and Chinese hamster chromosome 2p3 . 5 > p3 . 6 . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
The Ku heterodimer ( Ku 70 and Ku 80 subunits ) contributes to genomic integrity through its ability to bind DNA double strand breaks and facilitate repair by the non homologous end joining pathway . ^^^ Ku 70 and Ku 80 share a common topology and form a dyad symmetrical molecule with a preformed ring that encircles duplex DNA . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
DNA repair by nonhomologous end joining ( NHEJ ) relies on the Ku 70 : Ku 80 heterodimer in species ranging from yeast to man . ^^^ Here , we show that Ku 70 , Ku 80 , and DNA PKcs , with which Ku interacts , associate in vivo with telomeric DNA in several human cell types , and we show that these associations are not significantly affected by DNA damaging agents . ^^^ We also demonstrate that inactivation of Ku 80 or Ku 70 in the mouse yields telomeric shortening in various primary cell types at different developmental stages . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
In vitro , DNA PK undergoes phosphorylation of all three protein subunits ( DNA PK catalytic subunit , Ku 70 and Ku 80 ) and phosphorylation correlates with inactivation of the serine / threonine protein kinase activity of DNA PK . ^^^ In vivo phosphorylation of DNA PKcs , Ku 70 , and Ku 80 was observed when cells were labeled with [ ( 32 ) P ] inorganic phosphate in the presence of the protein phosphatase inhibitor , okadaic acid . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
Consistent with this , the NHEJ proteins Ku 70 and Ku 80 were found to be bound to purified retroviral replication intermediates . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
DNA PK comprises a catalytic subunit ( DNA PKcs ) and a DNA binding Ku 70 and Ku 80 heterodimer . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
Broken DNA ends are rejoined by non homologous end joining ( NHEJ ) pathways requiring the Ku proteins ( Ku 70 , Ku 80 ) , DNA ligase 4 and its associated protein Lif1 / Xrcc4 ( ref . 1 ) . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
XRCC 5 ( also known as Ku 80 ) is a component of the DNA dependent protein kinase ( DNA PK ) , existing as a heterodimer with G22P1 ( also known as Ku 70 ) . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
DNA dependent protein kinase ( DNA PK ) is composed of a 460 kDa catalytic subunit and the regulatory subunits Ku 70 and Ku 80 . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
Ku , composed of 70kDa ( ku 70 ) and 86kDa ( ku 80 ) proteins , is the DNA targeting subunit of the DNA dependent serine / threonine kinase ( DNA PK ) , which plays a crucial role in DNA double strand break recognition and repair in mammalian cells . ^^^ This cytokine effect was correlated with a greater amount of phosphorylated ku 80 , rather than increased expression of ku 70 and ku 80 proteins . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
DNA dependent protein kinase ( DNA PK ) , composed of Ku 70 , Ku 80 , and the catalytic subunit ( DNA PKcs ) , is involved in repairing double strand breaks ( DSBs ) by nonhomologous end joining ( NHEJ ) . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
Differential expression of DNA nonhomologous end joining proteins Ku 70 and Ku 80 in melanoma progression . ^^^ Ku 70 and Ku 80 heterodimers function as regulatory subunits of the DNA dependent protein kinase and play a very important role in the repairing of DNA double strand breaks . ^^^ Although Ku 70 is proposed as a candidate for a tumor suppressor gene , not many data are available on Ku 70 and Ku 80 expression in human tumors . ^^^ The main aim of this study was to investigate the expression of Ku 70 and Ku 80 in the ultraviolet induced lesions nevus cell nevi , lentigos maligna , and malignant melanomas . ^^^ Nineteen nevus cell nevi , 23 lentigos maligna , 76 primary melanomas , and 31 melanoma metastases were stained immunohistochemically for the presence of Ku 70 and Ku 80 proteins . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
After exposure to the same single doses , the mRNA levels of DNA PK catalytic subunit ( PKcs ) , Ku 70 , and Ku 80 were similar . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
Correlations between Ku 70 and Ku 80 expression were lost ( p > 0 . 05 ) . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
It is a three protein complex consisting of a 470 kDa catalytic subunit ( DNA PKcs ) and the regulatory DNA binding subunits , Ku heterodimer ( Ku 70 and Ku 80 ) . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
We report the molecular characterization of the KU 70 and KU 80 genes in Arabidopsis and describe the consequences of a Ku 70 deficiency . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
NHEJ can be catalyzed by mammalian whole cell extract in a reaction dependent upon DNA ligase 4 , Xrcc 4 , Ku 70 , Ku 80 , and DNA PKcs . ^^^ Cell extracts derived from Brca 1 deficient mouse embryonic fibroblasts exhibit reduced end joining activity independent of the endogenous protein amounts of DNA ligase 4 , Ku 80 , and Ku 70 . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
We now identify Ku 70 , Ku 80 , and Tbdn 100 , a variant of Tubedown 1 , as constituents of the purified OCFRE binding complex . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
Identification of Ku 70 and Ku 80 homologues in Arabidopsis thaliana : evidence for a role in the repair of DNA double strand breaks . ^^^ On the basis of their predicted sequence similarity to human Ku 70 and Ku 80 , cDNAs encoding the first plant homologues of these proteins ( AtKu 70 and AtKu 80 , respectively ) have now been isolated from Arabidopsis thaliana . ^^^ AtKu 70 and AtKu 80 share 28 . 6 and 22 . 5 % amino acid sequence identity with human Ku 70 and Ku 80 , respectively . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
The Ku 70 subunit specifically interacts with the N terminus ( amino acids 1 368 ) of WRN , while the Ku 80 subunit interacts with its C terminus ( amino acids 940 1432 ) . ^^^ A mutant Ku heterodimer of full length Ku 80 and truncated Ku 70 ( amino acids 430 542 ) interacts with C WRN but not with N WRN and can not stimulate WRN exonuclease activity . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
In yeast and mammals , the exposed ends of damaged DNA are bound with high affinity by a dimer of Ku 70 and Ku 80 proteins , which protects the ends from exonucleases and juxtaposes the two ends of the DSB , independent of sequence homology . ^^^ Here we report the functional characterization of Ku 70 and Ku 80 from Arabidopsis thaliana , and demonstrate that AtKu 80 and AtKu 70 form a heterodimer with DNA binding activity that is specific for DNA ends . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
DNA PK is composed of a large catalytic subunit ( DNA PKcs ) and a heterodimer of Ku 70 and Ku 80 that assemble on the ends of double stranded DNA to form an active serine / threonine protein kinase complex . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
LOH was identified in the specific coding region of DNA PK in 39 . 29 % for the DNA PK catalytic subunit ( cs ) , 17 . 86 % for Ku 70 , and only 7 . 14 % for Ku 80 . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
Expression of Ku 70 and Ku 80 mediated by NF kappa B and cyclooxygenase 2 is related to proliferation of human gastric cancer cells . ^^^ Inactivating Ku 70 or Ku 80 suppresses cell growth and induces apoptosis . ^^^ It has been hypothesized that Ku 70 and Ku 80 expression may be associated with NF kappaB activation and COX 2 expression and is involved in cell proliferation . ^^^ In this study , we found that inhibition of constitutive NF kappaB ( by transfecting a mutated IkappaBalpha gene ) and of COX 2 ( by treatment with indomethacin and NS 398 ) suppressed Ku 70 and Ku 80 expression in cells . ^^^ Ku 70 or Ku 80 overexpression by transfection with the Ku 70 or Ku 80 expression gene , respectively , enhanced proliferation of cells with low NF kappaB levels . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
The DNA repair protein Ku ( a heterodimer of Ku 70 and Ku 80 subunits ) associates with mammalian telomeres and contributes to telomere maintenance . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
DNA PK is composed of a large catalytic subunit , DNA PKcs , and a heterodimer of Ku 70 and Ku 80 subunits . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
Dimerization , translocation and localization of Ku 70 and Ku 80 proteins . ^^^ The Ku protein is a complex of two subunits , Ku 70 and Ku 80 , and was originally identified as an autoantigen recognized by the sera of patients with autoimmune diseases . ^^^ To clarify the fundamental characteristics of Ku , we have examined the expression , heterodimerization , subcellular localization , chromosome location , and molecular mechanisms of the nuclear transport of Ku 70 and Ku 80 . ^^^ The mechanism that regulates for nuclear localization of Ku 70 and Ku 80 appears to play , at least in part , a key role in regulating the physiological function of Ku in vivo . . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
Ku 80 with the same activation domain had no effect on transcription when expressed alone , but reconstituted activation when co expressed with native human Ku 70 . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
Cells of mouse knockout cell lines for Ku 80 ( now known as Xrcc 5 ) , Ku 70 ( now known as G22p1 ) , DNA PKcs ( now known as Prkdc ) and PARP ( now known as Adprt ) were synchronized in G 1 phase and exposed to very low fluences of alpha particles . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
Detailed examination indicates that the patient is defective neither in the known factors involved in NHEJ in mammals ( Ku 70 , Ku 80 , DNA dependent protein kinase catalytic subunit , Xrcc 4 , DNA ligase 4 , or Artemis ) nor in the Mre11 / Rad50 / Nbs1 complex , whose homologue in Saccharomyces cerevisiae functions in NHEJ . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
Ku 80 deficiency results in the lengthening of telomeres , a phenotype also seen in an Arabidopsis ku 70 mutant . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
The basal mtHsp 70 and Hsp 60 levels of Ku 70 ( / ) cells , which were known to be sensitive to anticancer drugs , were higher than those of parental MEF cells , but conversely these mitochondrial heat shock proteins of R 7080 6 cells over expressing both Ku 70 and Ku 80 were lower than those of parental Rat 1 cells . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
OBJECTIVE : To examine the expression of DNA dependent protein kinase ( DNA PK ) ; including DNA PKcs , Ku 70 and Ku 80 in human neuroectodermal tumor cells with or without retroviral DNA integration . ^^^ RESULTS : The expression of DNA PKcs was similar in the cells with or without retroviral DNA integration , but the expression of Ku ( both mRNA and protein for Ku 70 and only protein for Ku 80 ) was higher in cells with retroviral DNA integration than in cells without retroviral DNA integration . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
In analyzing the DNA repair capacity of C 70 and C 200 , an altered expression of the DNA dependent protein kinase ( DNA PK ) complex ( catalytic subunit DNA PKcs , and the heterodimers Ku 70 and Ku 80 ) was found . ^^^ In C 70 and C 200 cells , DNA PKcs was overexpressed at both the transcript and protein levels , whereas amounts of Ku 70 and Ku 80 were higher only at the level of protein expression . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
Ku 70 protein , cooperating with Ku 80 and DNA dependent protein kinase ( DNA PK ) catalytic subunit ( DNA PKcs ) , is involved in DNA double strand break ( DNA DSB ) repair and 5 ( D ) J recombination . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
Ku 70 is one component of a protein complex , Ku 70 and Ku 80 , that functions as a heterodimer to bind DNA double strand breaks and activates DNA dependent protein kinase . ^^^ Our previous study with Ku 70 / and Ku 80 / mice , and cell lines has shown that Ku 70 and Ku 80 deficiency compromises the ability of cells to repair DNA double strand breaks , increases radiosensitivity of cells , and enhances radiation induced apoptosis . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
Oxidative stress induces nuclear loss of DNA repair proteins Ku 70 and Ku 80 and apoptosis in pancreatic acinar AR42J cells . ^^^ It has been hypothesized that oxidative stress may induce a decrease in the Ku 70 and Ku 80 levels and apoptosis in pancreatic acinar cells . ^^^ In this study , it was found that oxidative stress caused by glucose oxidase ( GO ) acting on beta d glucose , glucose / glucose oxidase ( G / GO ) , induced slight changes in cytoplasmic Ku 70 and Ku 80 but drastically induced a decrease in nuclear Ku 70 and Ku 80 both time and concentration dependently in AR42J cells . ^^^ G / GO did not induce apoptosis in the cells transfected with either the Ku 70 or Ku 80 expression gene but increased apoptosis in those transfected with the Ku dominant negative mutant . ^^^ Pulse and pulse chase results show that G / GO induced Ku 70 and Ku 80 syntheses , even though Ku 70 and Ku 80 were degraded both in cytoplasm and nucleus . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
Several reports indicate that the Ku 70 and Ku 80 subunits of the mammalian DNA dependent protein kinase ( DNA PK ) , a complex involved in NHEJ , appear to be susceptible to a heat induced loss of DNA binding activity , with Ku 80 representing the heat sensitive component . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
We did not observe any abnormalities in expression level or migration pattern of the following NHEJ proteins in radiosensitive cancer cases : Ku 70 , Ku 80 , XRCC 4 , DNA Ligase 4 . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
The non homologous end joining ( NHEJ ) pathway of DNA recombination is important for genomic stability in animal cells , since the absence of Ku 70 , Ku 80 , Lig 4 or Xrcc 4 results in non reciprocal translocation and chromosome fragmentation . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
The intracellular distribution and amount of the DNA dependent protein kinase ( DNA PK ) complex components ( ku 70 , ku 80 , DNA PKcs ) , which are involved in the recognition and repair of DNA breaks caused by ionizing radiations , 5 ( D ) J recombination and isotype switching , was assessed in nai ; ve and memory T cells from young subjects . ^^^ While the expression of ku 70 and ku 80 was at comparable levels in both T cell subsets , DNA PKcs , phosphorylated ku 80 , and DNA binding of ku 70 / 80 were mostly evident in nai ; ve but negligible or absent in memory T cells . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
Because single nucleotide polymorphisms ( SNPs ) are the most subtle genetic variation in the genome , to examine these hypotheses , we have genotyped 30 SNPs in all five NHEJ genes ( Ku 70 , Ku 80 , DNA PKcs , Ligase 4 , and XRCC 4 ) in 254 primary breast cancer patients and 379 healthy controls . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
The main components of the NHEJ system in eukaryotes are the catalytic subunit of DNA protein kinase ( DNA PK ( cs ) ) , which is recruited by DNA Ku protein , a heterodimer of Ku 70 and Ku 80 , as well as XRCC 4 protein and DNA ligase 4 . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
Application of new in situ hybridization probes for Ku 70 and Ku 80 in tissue microarrays of paraffin embedded malignant melanomas : correlation with immunohistochemical analysis . ^^^ Ku 70 and Ku 80 proteins are responsible for the repair of DNA double strand breaks and function as a regulatory subunit of the DNA dependent protein kinase . ^^^ In situ hybridization demonstrated more Ku 70 and Ku 80 positive cells than immunohistochemical methods , but the correlation between the two methods was highly significant ( P < 0 . 01 ) . ^^^ We conclude that the in situ hybridization assay for the detection of Ku 70 and Ku 80 expression used in this study is also suitable for tissue microarray analysis of paraffin embedded melanoma samples . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
The observed increase in cell adhesion relied on Ku cell surface expression , since antibodies directed against Ku 70 or Ku 80 subunit inhibited adhesion on Fn of Ku 80 , but not control vector , transfected xrs 6 cells . ^^^ In addition , both Ku 70 and Ku 80 present a structural relationship with integrin 1 ( or A ) domains and the A 1 and A 3 domains of von Willebrand factor , domains known to be involved in Fn binding . ^^^ Both Ku 70 and Ku 80 exhibit a complete set of residues compatible in their position and chemical nature with the formation of a metal ion dependent adhesion ( MIDAS ) site implicated in ligand binding and integrin activation . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
We showed that the modulation of Ku activity during the aging process was highly tissue specific as shown with highly impaired Ku activity in testis and unaffected Ku activity in liver with age , and the level of Ku 70 or Ku 80 was differentially expressed in each aging tissue . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
DNA PK is a nuclear protein with serine / threonine kinase activity and forms a complex consisting of the DNA PKcs and a heterodimer of Ku 70 and Ku 80 proteins . ^^^ In this study , we evaluated the relationship between expression levels of DNA PKcs , Ku 70 and Ku 80 proteins and radiation sensitivity in oral squamous cell carcinoma ( OSCC ) cell lines and in OSCC patients treated with preoperative radiation therapy . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
DNA dependent protein kinase ( DNA PK ) , composed of Ku 70 , Ku 80 , and the catalytic subunit ( DNA PKcs ) , is involved in double strand break ( DSB ) repair by non homologous end joining ( NHEJ ) . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
Among the genes in this region , hid plays a central , dosage sensitive role in IR induced apoptosis . p 53 and MNK / Chk2 also regulate DNA repair genes , including two components of the nonhomologous end joining repair pathway , Ku 70 and Ku 80 . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
Two of these proteins , Ku 70 and Ku 80 , were identified by immunoblot analysis . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
We show by mass spectrometry that the DNA binding component of human OF 1 contains Ku 70 and Ku 80 proteins . . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
Agnoprotein expression correlated with impaired double strand break repair activity in cellular extracts and reduced expression of the Ku 70 and Ku 80 DNA repair proteins . ^^^ Since Ku 70 is a subunit of the DNA dependent protein kinase that is responsible both for double strand break repair and for signaling damage induced cell cycle arrest , the modulation of Ku 70 and / or Ku 80 by agnoprotein may represent an important event in the polyomavirus life cycle and in cell transformation . . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
During DNA damage , DNA repair proteins Ku 70 and Ku 80 prevent cell death , but severe DNA damage beyond the repair capacity of the DNA repair proteins triggers necrosis or apoptosis . ^^^ G / GO treatment caused nuclear loss of both Ku 70 and Ku 80 in Wild cells and pcN 3 cells . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
This signal is produced within the damaged cell by the kinase function of the Ku 70 , Ku 80 , and DNA dependent protein kinase complex and is conveyed to the recipient cell by direct cell to cell communication through gap junctions . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
These genes included G22P1 ( Ku 70 ) and XRCC 5 ( Ku 80 ) genes known as important members of the nonhomologous end joining ( NHEJ ) pathway of DNA double strand break ( DSB ) repair . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
The Ku protein is a complex of two subunits , Ku 70 and Ku 80 , and it plays a role in multiple nuclear processes , e . g . , nonhomologous DNA end joining ( NHEJ ) , chromosome maintenance , and transcription regulation . ^^^ The mechanism underlying the regulation of all the diverse functions of Ku is still unclear , though the mechanism that regulates the nuclear localization of Ku 70 and Ku 80 appears to play , at least in part , a key role in regulating the physiological function of Ku . ^^^ Although Ku 70 , as well as Ku 80 , was undetectable in xrs 6 cells , it was seen in these transformants at a level similar to the level of CHO K 1 . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
Ku 70 , Ku 80 , DNA PKcs ( the catalytic subunit of DNA dependent protein kinase ) , Xrcc 4 and Artemis all participate in DSB repair through nonhomologous end joining ( NHEJ ) . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
The levels of ku 70 , ku 80 , DNA PKcs , phosphorylated ku 80 as well as the DNA binding activity of the ku70 / ku 80 heterodimer were determined in the cytoplasmic and nuclear extracts obtained , before and after irradiation , from young and elderly subjects . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
RESULTS : Using both supershift and Western analysis , the following proteins were identified in the DNA end binding complex : Ku 70 , Ku 80 , DNA dependent protein kinase catalytic subunit , DNA ligase 4 , 10 ray cross complementing protein 4 , meiotic recombination protein 11 ( Mre 11 ) , Werner ' s syndrome protein , Bloom ' s syndrome protein , p 53 , poly ( ADP ribose ) polymerase , replication protein A ( RPA ) 14 , and RPA 32 , ataxia telangiectasia mutant , c Abl , Rad 50 , Nijmegen breakage syndrome protein 1 ( NBS 1 ) , and DNA ligase 3 were not detected in the binding complex by any assay . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
We identified and disrupted Neurospora crassa genes homologous to human KU 70 and KU 80 , which encode proteins that function in nonhomologous end joining of double stranded DNA breaks . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
Furthermore , we succeeded in visualizing that Ku 80 rapidly assembled to the exposed region , in which DSBs might be generated , and clarified that the presence of both Ku 70 and Ku 80 was important for their accumulation . . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
In this report , we demonstrate that Ku , a heterodimer of p 80 ( Ku 80 ) and p 70 ( Ku 70 ) , is upregulated in P 19 cells overexpressing Galpha13Q226L . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
Through peptide mass spectrometry , we identified this binding activity to be a heterodimer of Ku 70 and Ku 80 . ^^^ Interestingly , YY 1 interacts with Ku 70 and Ku 80 in HeLa cells . ^^^ Together , YY 1 , Ku 70 , and Ku 80 repress the alphaMyHC promoter to an extent that is greater than that with YY 1 or Ku70 / 80 alone . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
Exposure of LM 217 and MDA MB 468 cells to suramin did not affect the level of Ku 70 ( G22P1 ) or Ku 80 ( XRCC 5 ) , but it increased the level of DNA PKcs ( PRKDC ) . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
In normal and tumoral migratory cells , Ku 80 and MMP 9 colocalize at the periphery of leading edge of cells and cellular invasion of collagen 4 matrices was blocked by antibodies directed against Ku 70 or Ku 80 subunits as well as by Ku 80 specific antisense oligonucleotides . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
The budding yeast Saccharomyces cerevisiae repairs DSB primarily by homologous recombination ( HR ) , despite the presence of the KU 70 , KU 80 , DNA ligase 4 and XRCC 4 homologues , essential factors of the mammalian non homologous end joining ( NHEJ ) machinery . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
Down regulation of Ku 70 and Ku 80 mRNA expression in transitional cell carcinomas of the urinary bladder related to tumor progression . ^^^ DNA dependent protein kinase ( DNA PK ) containing the regulatory subunits Ku 70 and Ku 80 plays a prominent role in the repair of double stranded DNA breaks by a nonhomologous end joining pathway maintaining genomic stability . ^^^ In an attempt to elucidate the significance of the DNA PK complex for human urothelial carcinogenesis , the expression of Ku 70 and Ku 80 was studied in 71 transitional cell carcinomas ( TCC ) of the urinary bladder of various grades and stages , and in relation to lifestyle and occupational bladder cancer risk factors . ^^^ To analyse the mRNA expression of Ku 70 and Ku 80 , real time quantitative reverse transcription polymerase chain reaction was used and the protein expression assessed by immunohistochemistry . ^^^ Advanced high grade , high stage TCC expressed the mRNA of Ku 70 and Ku 80 at a lower level than superficial low grade , low stage carcinomas , suggesting down regulation of the Ku system to be associated with progression of bladder cancer from a low to a high malignant potential . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
The core components of mammalian NHEJ are the catalytic subunit of DNA protein kinase ( DNA PK ( cs ) ) , Ku subunits Ku 70 and Ku 80 , Artemis , XRCC 4 and DNA ligase 4 . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
KU is a heterodimer , consisting of approximately 70 and approximately 80 kDa subunits ( KU 70 and KU 80 , respectively ) , which is involved in a variety of nuclear functions . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
AR directly interacts with both Ku 70 and Ku 80 in vivo and in vitro , as shown by co immunoprecipitation , glutathione S transferase pull down , and Sf 9 cell / baculovirus expression . ^^^ Chromatin immunoprecipitation experiments demonstrated that both Ku 70 and Ku 80 interact with the prostate specific antigen promoter in an androgen dependant manner . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
Endogenous Chk 2 coimmunoprecipitates Ku 70 and Ku 80 . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
Here we show that a decrease in Ku 70 or Ku 80 gene dosage causes a sharp increase in the frequency of HeT A and TART attachments to a broken chromosome end and in terminal DNA elongation by gene conversion . ^^^ Loss of Ku 80 has more pronounced effects than loss of Ku 70 . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
The Ku 70 binding site of Ku 80 is required for the stabilization of Ku 70 in the cytoplasm , for the nuclear translocation of Ku 80 , and for Ku 80 dependent DNA repair . ^^^ It is believed that heterodimerization between Ku 70 and Ku 80 is essential for Ku dependent DNA repair , although its role is poorly understood . ^^^ We previously identified the Ku 70 binding site of Ku 80 . ^^^ In this study , to understand the role of heterodimerization in the function of Ku , we generated and / or analyzed cell lines stably expressing the EGFP tagged wild type human Ku 80 , its Ku 70 binding mutant , its NLS dysfunctional mutant , or its double mutant in Ku 80 deficient cells . ^^^ Our results show that the Ku 70 binding site of Ku 80 is required for the stabilization of Ku 70 in the cytoplasm and for the nuclear translocation of Ku 80 through its heterodimerization with Ku 70 . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
By immunoprecipitation and using the matrix assisted laser desorption / ionization time of flight mass spectrometry approach , the antigenic specificity of CLD 3 was determined to be heterodimeric Ku 70 and Ku 80 autoantigen , which was confirmed by Western blotting . . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
OBJECTIVES : Ku , a heterodimer of KU 70 and Ku 80 that binds to double strand DNA breaks ( DSBs ) and activates the catalytic subunit ( DNA PKcs ) when DNA is bound , is essential in DSB repair and 5 ( D ) J recombination . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
We previously identified four DNA PK phosphorylation sites on the Ku70 / 80 heterodimer : serine 6 of Ku 70 , serine 577 and 580 and threonine 715 of Ku 80 . ^^^ Interestingly , both Ku 70 and Ku 80 were phosphorylated in cells treated with the protein phosphatase inhibitor okadaic acid under conditions known to inactivate protein phosphatase 2A like protein phosphatases . ^^^ These results suggest that the phosphorylation of Ku 70 and Ku 80 is regulated by a protein phosphatase 2A like protein phosphatase and a staurosporine sensitive protein kinase in vivo , but that DNA PK mediated phosphorylation of Ku is not required for DNA double strand break repair . . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
Western blot analyses revealed a 75 % and 36 % decrease in the nuclear expression of Ku 80 and Ku 70 , respectively . ^^^ Chromatin immunoprecipitation ( ChIP ) assays showed that the association of Ku 80 with the lamin B 2 , beta globin and c myc origins was decreased by 1 . 5 , 2 . 3 and 2 . 5 fold , respectively , whereas that of Ku 70 was similarly decreased ( by 2 . 1 , 1 . 5 and 1 . 7 fold , respectively ) in Ku80+ / cells . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
These proteins , Ku 70 and Ku 80 , are regulatory subunits of DNA dependent protein kinase ( DNA PK ) . ^^^ We determined that the interaction between PDX 1 and Ku 70 or Ku 80 is dependent on the homeodomain of PDX 1 . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
DNA double strand breaks ( dsb ) during 5 ( D ) J recombination of T and B lymphocyte receptor genes are resolved by the non homologous DNA end joining pathway ( NHEJ ) including at least six factors : Ku 70 , Ku 80 , DNA PK ( cs ) , Artemis , Xrcc 4 , and DNA ligase 4 ( Lig 4 ) . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
Identification and analysis of Ku 70 and Ku 80 homologs in the koji molds Aspergillus sojae and Aspergillus oryzae . ^^^ We have identified Ku 70 and Ku 80 homologs in the koji molds Aspergillus sojae and Aspergillus oryzae , and have constructed the disruption mutants of Ku 70 , Ku 80 , and Ku 70 80 to characterize the phenotypic change in these mutants . ^^^ Neither Ku 70 nor Ku 80 disrupted strains show hypersensitivity to the DNA damaging agents methylmethane sulfonate ( MMS ) and phleomycin . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
Enhanced gene targeting frequency in ku 70 and ku 80 disruption mutants of Aspergillus sojae and Aspergillus oryzae . ^^^ In the koji molds Aspergillus sojae and Aspergillus oryzae , exogenous DNA is integrated in the genome , in most cases irrespective of the sequence homology , suggesting that DNA integration occurs predominantly through a nonhomologous end joining pathway where two ku genes , namely , ku 70 and ku 80 , play a key role . ^^^ To determine the effect of ku gene disruption on the gene targeting frequency , we constructed ku 70 , ku 80 , and ku 70 ku80 disrupted strains of A . sojae and A . oryzae . ^^^ The gene targeting frequency of the tannase gene in ku 70 and ku 80 strains of both Aspergillus species was markedly enhanced as compared with that of the parental strains . ^^^ The gene targeting frequency of the aflR and ku 80 genes was also enhanced in an A . sojae ku 70 background . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
Ku is a heterodimeric protein made up of two subunits , Ku 70 and Ku 80 . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
LOH was recurrently observed for 8 of the 9 studied genes ( KU 70 , KU 80 , XRCC 4 , LIG 4 , Artemis , MRE 11 , RAD 50 , NBS 1 ) but not for DNA PKcs . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
In prostate cancer cells the interaction of C / EBPalpha with Ku 70 , Ku 80 , and poly ( ADP ribose ) polymerase 1 increases sensitivity to DNA damage . ^^^ Ku 70 , Ku 80 , and poly ( ADP ribose ) polymerase 1 ( PARP 1 ) were identified as proteins that associated with C / EBPalpha . ^^^ Immunoprecipitation of C / EBPalpha expressed in prostate cancer cells co precipitated Ku 70 , Ku 80 , and PARP 1 . ^^^ Deletion analysis of C / EBPalpha indicated that the C terminus of C / EBPalpha was essential for the interaction of C / EBPalpha with Ku 70 , Ku 80 , and PARP 1 . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
To address this issue , we examined the expression pattern of DSB repair key proteins ATM , BRCA 1 , BRCA 2 , Ku 70 , and Ku 80 and their putative role in patients survival in a large series of colorectal cancer . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
Ku 70 and Ku 80 proteins take part in the repairing of DNA double strand breaks by their function as a regulatory subunit of the DNA dependent protein kinase . ^^^ Significant differences in Ku 70 and Ku 80 expression were found between pT 3 and pT 4 as well as between pT 2 and pT 3 tumors , respectively . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
Altered expression of DNA double strand repair genes Ku 70 and Ku 80 in carcinomas of the oral cavity . ^^^ The main aim of this study was to analyse the expression of the heterodimers Ku 70 and Ku 80 , building regulatory subunits of the DNA dependent protein kinase in 40 oral carcinomas . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
Esophageal cancer tissues and adjacent normal mucosae in 13 patients with primary esophageal cancer were examined for quantitative differences in DNA dependent protein kinase ( DNA PK ) activity and for expressions of Ku 70 , Ku 80 and DNA PKcs proteins by Western blotting and immunohistochemistry . ^^^ Protein levels of Ku 70 , Ku 80 and DNA PKcs correlated with DNA PK activities in the tumor tissues . ^^^ Immunohistochemical analysis revealed that Ku 70 , Ku 80 and DNA PKcs located predominantly in the nuclei in both the tumor tissues and normal mucosae . ^^^ In the normal epithelium , Ku 70 , Ku 80 and DNA PKcs were expressed only in the nuclei of the basal cell layers and not in those of the lumenal cell layers . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
Transfection of full length cDNA encoding hamster Ku 86 into XR V15B cells resulted in a complete rescue of DNA end binding ( DEB ) activity and Ku 70 levels , suggesting that Ku 86 stabilizes the Ku 70 polypeptide . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
Three proteins known to play a critical role in mammalian DNA double strand break repair and lymphoid 5 ( D ) J recombination are the autoantigens Ku 86 and Ku 70 and a 465 kDa serine / threonine protein kinase catalytic subunit ( DNA PKcs ) . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
Little is known of which regions of Ku 70 and Ku 86 bind to each other to form the heterodimeric complex or of which regions are important for DNA end binding . ^^^ We found that the C terminal 20 kDa region of Ku 70 and the C terminal 32 kDa region of Ku 86 are important for subunit subunit interaction . ^^^ DNA end binding activity by the heterodimer requires the C terminal 40 kDa region of Ku 70 and the C terminal 45 kDa region of Ku 86 . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
Recent studies suggest that the SCID defect lies in the gene encoding the catalytic subunit of DNA dependent protein kinase ( DNA PK ; refs 6 8 ) , a nuclear protein made up of the Ku 70 and Ku 86 subunits as well as the large catalytic subunit , DNA PKcs . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
The heterodimeric Ku protein , which comprises a 86 kDa ( Ku 86 ) amd a 70 kDa ( Ku 70 ) subunits , is an abundant nuclear DNA binding protein which binds in vitro to DNA termini without sequence specificity . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
The mammalian Ku 70 and Ku 86 proteins form a heterodimer that binds to the ends of double stranded DNA in vitro and is required for repair of radiation induced strand breaks and 5 ( D ) J recombination [ 1 , 2 ] . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
Intermolecular ligation was found to be accurate and to depend on DNA ligase IV / Xrcc4 and requires Ku 70 , Ku 86 , and DNA PKcs , the three subunits of the DNA activated protein kinase DNA PK . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
Previous studies have suggested that ionizing radiation causes irreparable DNA double strand breaks in mice and cell lines harboring mutations in any of the three subunits of DNA dependent protein kinase ( DNA PK ) ( the catalytic subunit , DNA PKcs , or one of the DNA binding subunits , Ku 70 or Ku 86 ) . ^^^ Mutant cell lines and mice with targeted deletions in Ku 70 or Ku 86 are severely compromised in their ability to form coding and signal joints , the products of 5 ( D ) J recombination . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
In mammals , nuclear DEB activity is due to Ku , a hetero dimer composed of the Ku 70 and Ku 86 proteins . ^^^ Although the nuclear and mitochondrial DEB activities are independent of each other , they are nevertheless closely related , since mitochondrial DEB activity was ' supershifted ' by both anti Ku 70 and anti Ku 86 antisera . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
DNA double strand breaks formed during the assembly of antigen receptors or after exposure to ionizing radiation are repaired by proteins important for nonhomologous end joining that include Ku 86 , Ku 70 , DNA PK ( CS ) , Xrcc 4 , and DNA ligase 4 . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
In fact , the levels of Ku 86 , Ku 70 and DNA end binding activity of Ku remained unchanged during replicative senescence . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
DNA PK plays a major role in the repair of double strand breaks induced in DNA after exposure to ionizing radiation as shown by the extreme radiosensitivity of cells with mutations in Ku 86 , Ku 70 or DNA PKcs genes . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
The three other proteins were identified as subunits of the DNA dependent protein kinase ( DNA PK ) holoenzyme : its DNA binding regulatory heterodimers consisting of Ku 70 and Ku 86 , and the 460 kDa catalytic subunit , DNA PK ( CS ) . ^^^ DNA PK that was ' pulled down ' by DBD ( PR ) on the affinity resin was able to ( 1 ) autophosphorylate Ku 70 , Ku 86 , and DNA PK ( CS ) , ( 2 ) transphosphorylate DBD ( PR ) , and ( 3 ) phosphorylate a DNA PK specific p 53 peptide substrate . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
Here we identify Ku 70 and Ku 86 as proteins that interact with WRNp . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
The affinity of one of the scFv antibodies for Ku 86 was 16 nM as measured by BIAcore analysis . scFv immunoprecipitation of Ku 86 also isolated the other component of the heterodimer , Ku 70 , as determined by Western analysis and mass spectrometry . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
Ku is a heterodimer of Ku 70 and Ku 86 that binds to double stranded DNA breaks ( DSBs ) , activates the catalytic subunit ( DNA PKcs ) when DNA is bound , and is essential in DSB repair and 5 ( D ) J recombination . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
Protein expression levels of the DNA dependent protein kinase ( DNA PK ) / Ku dependent nonhomologous DNA end joining proteins Xrcc 4 , DNA ligase 4 , Ku 70 , and Ku 86 in FA and normal extracts were indistinguishable , as were DNA dependent protein kinase and DNA end binding activities . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
Ku 86 together with Ku 70 , DNA PKcs , XRCC 4 and DNA ligase 4 forms a complex involved in repairing DNA double strand breaks ( DSB ) in mammals . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
In the present work the Ku 70 and Ku 86 dimer proteins in T and B lymphocytes from human blood donors were analysed by immunoblotting and were observed apparently to be of full length . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
Early decrease in dna repair proteins , Ku 70 and Ku 86 , and subsequent DNA fragmentation after transient focal cerebral ischemia in mice . ^^^ BACKGROUND AND PURPOSE : Ku 70 and Ku 86 , multifunctional DNA repair proteins , bind to broken DNA ends , including double strand breaks , and trigger a DNA repair pathway . ^^^ Western blot analyses confirmed the significant reduction of these proteins ( 59 . 4 % and 57 . 7 % reduction in optical density at 4 hours of reperfusion from the normal level of Ku 70 and Ku 86 bands , respectively ; P < 0 . 001 ) . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
The heterodimeric Ku protein ( composed of the Ku 86 and Ku 70 sub units ) is a nuclear protein which binds to DNA termini without sequence specificity . ^^^ We further demonstrate that immunoprecipitation of Ku is impaired in extracts from differentiated cells by using two antibodies that recognize epitopes within the C terminus DNA binding domains of Ku 70 and Ku 86 proteins . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
Nonhomologous endjoining was investigated by determining the levels of Ku 70 and Ku 86 proteins and DNA PK activity . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
DNA dependent protein kinase ( DNA PK ) , consisting of a catalytic subunit ( DNA PKcs ) and the Ku 70 and Ku 86 proteins , participates in the repair of DNA double strand breaks ( DSBs ) . ^^^ Compared with the expression in normal tissue [ 176 . 62 + / 18 . 56 ( the intensity of expression 10 the percentage of cells expressing this protein ) , mean + SE ] , the expression of Ku 70 was significantly reduced in adenomas ( 36 . 62 + / 11 . 09 ; P < 0 . 001 ) and carcinomas ( 85 . 68 + / 15 . 76 ; P < 0 . 01 ) , as was the expression of Ku 86 [ ( 113 . 10 + / 10 . 22 versus 41 . 66 + / 14 . 71 in adenomas ( P < 0 . 01 ) or versus 85 . 68 + / 15 . 76 in carcinomas ( P < 0 . 05 ) ] . ^^^ The marked underexpression of Ku 70 and Ku 86 starting at the adenoma stage may be crucial to the development of colon cancer . . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
Functional inactivation in rodents of either Ku 86 or Ku 70 , which form the heterodimeric DNA end binding subunit of the DNA dependent protein kinase complex , is nevertheless compatible with viability . ^^^ In contrast , no human patient has been described with mutations in either Ku 86 or Ku 70 . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
The DNA end binding protein Ku ( an equimolar mixture of Ku 70 , now known as G22P1 , and Ku 86 , now known as XRCC 5 ) did not alter the resistance of the 3 ' phosphoglycolate substrates , but the protein modulated the susceptibility of 3 ' hydroxyl substrates , allowing DNase 3 to remove a 3 ' overhang but inhibiting digestion of the double stranded portion of the substrate . . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
The autoantigen Ku , composed of subunits Ku 70 and Ku 86 , is necessary for repair of DNA double strand breaks by nonhomologous end joining . ^^^ To address this question , fluorescence photobleaching experiments were performed using HeLa cells and B cells expressing a green fluorescent protein ( GFP ) fusion construct of either Ku 70 or Ku 86 . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
OBJECTIVE : Since the central hallmarks of human multiple myeloma ( MM ) are abnormalities in immunoglobulin ( Ig ) gene rearrangement , IgH class switching , and DNA damage repair , and since Ku 86 and Ku 70 proteins are central to these processes , aberrant Ku function may play a role in MM pathogenesis . ^^^ MATERIALS AND METHODS : We examined Ku 86 and Ku 70 translocation following CD 40 activation in human MM cells vs normal tonsillar B lymphocytes . ^^^ We then identified the functional sequelae of membrane Ku 86 and Ku 70 expression on CD 40 activated human MM cells . ^^^ RESULTS : CD 40 activation induces translocation of both Ku 86 and Ku 70 to the cell surface of MM cells , but not normal tonsillar B cells . ^^^ Therefore , targeting Ku 86 and Ku 70 , with blocking peptides for example , might serve as a novel treatment strategy in human MM . . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
Strikingly , we show that the NHEJ proteins , Ku 70 and 86 , are required for the deletions in myeloid leukemias because preincubating nuclear extracts from leukemic cells with antisera against Ku 86 and Ku 70 inhibits plasmid reactivation and restores the frequency and size of deletions to control levels . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
Blimp 1 also inhibited immunoglobulin class switching by blocking expression of AID , Ku 70 , Ku 86 , DNA PKcs , and STAT 6 . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
In AML samples , sensitivity to DNA cross linking alkylating agents correlated with Ku 86 ( r= 0 . 7512 , p=0 . 0031 ) and Ku 70 ( r= 0 . 6134 , p=0 . 0258 ) expression . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
The gels were then processed for Western blotting using the Ku 70 , Ku 86 and DNA PKcs antibodies . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
RESULTS : There was a high degree of correlation between the percentage of cells that expressed the 70 kDa Ku protein ( Ku 70 ) and the 86 kDa Ku protein ( Ku 86 ) in the tumor sections ( correlation coefficient = 0 . 85 ; P < 0 . 001 ) . ^^^ CONCLUSIONS : Ku 70 and Ku 86 raise the predictive possibility of tumor radiosensitivity . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
Ku protein , a heterodimer of 70kDa ( Ku 70 ) and 86kDa ( Ku 86 ) polypeptides , is involved in non homologous DNA end joining ( NHEJ ) of DNA double strand break repair and 5 ( D ) J recombination in combination with the catalytic component of DNA dependent protein kinase ( p 470 ) . ^^^ Transcripts of Ku 70 and Ku 86 genes were also detected by the reverse transcriptase polymerase chain reaction ( RT PCR ) , and Ku protein was shown to be localized in the nucleus of neutrophils as a heterodimer . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
Analysis by mass spectrometric protein sequencing revealed a DNA dependent protein kinase ( DNA PK ) complex including its catalytic subunit and regulatory subunits , Ku 70 and Ku 86 . ^^^ DNA PK phosphorylates TRBP at its C terminal region , which directly interacts with Ku 70 but not Ku 86 in vitro . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
Ku is a heterodimeric protein consisting of Ku 70 and Ku 86 , and it is thought to be the first component to bind to a broken double strand DNA end . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
Function of DNA protein kinase catalytic subunit during the early meiotic prophase without Ku 70 and Ku 86 . ^^^ All components of the double stranded DNA break ( DSB ) repair complex DNA dependent protein kinase ( DNA PK ) , including Ku 70 , Ku 86 , and DNA PK catalytic subunit ( DNA PKcs ) , were found in the radiosensitive spermatogonia . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
Six components function in this pathway , of which three ( Ku 70 , Ku 86 , and DNA PKcs ) constitute a protein complex known as DNA dependent protein kinase ( DNA PK ) . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
Importantly , damaged foci from myeloid leukemia and normal cells colocalize with NHEJ proteins Ku 70 and Ku 86 . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
TdT synthesizes the N region in concert with many proteins including DNA PKcs , Ku 70 and Ku 86 . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
In addition , flavopiridol treatment ( 300 nM , 1 day ) resulted in decreased levels of Ku 70 and Ku 86 proteins that play a role in DNA repair processes , suggesting that DNA repair processes may have been disrupted by this agent . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
The decreased kinase activity of DNA PKcs was not due to a change in its amount or the levels of Ku 70 and Ku 86 , their subcellular distribution , cell cycle progression or caspase mediated degradation of DNA PK . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
Ku 70 and Ku 86 together with DNA PKcs form the DNA dependent protein kinase ( DNA PK ) complex that is involved in DNA double strand break repair by nonhomologous end joining . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
Amino acid sequence analysis demonstrated that three proteins , DNA PK catalytic subunit , Ku 86 , and Ku 70 are components of DNA dependent protein kinase ( DNA PK ) . ^^^ AREB 6 like proteins specifically bind to the E box , whereas Ku 86 binds an adjacent site and recruits DNA PK catalytic subunit and Ku 70 proteins . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
The Ku heterodimer , which consists of Ku 70 and Ku 86 subunits , is a major sensor of DNA breaks . ^^^ The resulting Ku 86 variant exists exclusively in complexes with Ku 70 , which possess strong affinity to DNA double strand termini . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
We have determined that Ku 70 , Ku 86 , p 300 , and CREB binding protein ( CBP ) are ESE 1 interacting proteins . ^^^ Interestingly , Ku 70 and Ku 86 negatively regulate the transcriptional activity of ESE 1 . ^^^ In summary , our results demonstrated that the activity of ESE 1 is positively and negatively modulated by other interacting proteins including Ku 70 , Ku 86 , p 300 , and CBP . . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
Expression of DNA PKcs and Ku 86 , but not Ku 70 , differs between lymphoid malignancies . ^^^ Low grade lymphoma , appearing as chronic lymphocytic leukaemia ( CLL ) displayed a significantly lower frequency of cells staining positive for DNA PKcs and Ku 86 , but surprisingly not for Ku 70 , compared with acute lymphoblastic leukaemia ( ALL ) cells . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
Ku protein is a heterodimer ( Ku 70 and Ku 86 ) known to play an important role in 5 ( D ) J recombination , apoptosis , telomere fusion , and double strand break repair . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
The expression of p 53 , p 21 and p 16 in more than 5 % of tumor cells were defined as positive , whereas both Ku 70 and Ku 86 protein in more than 70 % of such cells were defined as Ku positive . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
Moreover , NaB significantly reduced the expression of the repair related genes Ku 70 and Ku 86 and DNA dependent protein kinase catalytic subunit in melanoma cells at the protein and mRNA levels . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
There was no change in the protein levels of DNA PKcs , Ku 70 , or Ku 86 . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
Despite the observed defects in the repair efficiency , no apparent differences in homologous recombination or non homologous end joining effector proteins RAD 51 , KU 70 , KU 86 , or DNA PK ( CS ) were observed . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
Ku , the heterodimer of Ku 70 and Ku 86 , plays crucial roles in non homologous end joining ( NHEJ ) , a major pathway for repairing DNA double strand breaks ( DSBs ) in mammalian cells . ^^^ In this paper , however , we show that heterozygous inactivation of either the KU 70 or KU 86 gene does not cause any defects in cell proliferation or DSB repair in human somatic cells . ^^^ Moreover , although these heterozygous cell lines express reduced levels of both Ku 70 and Ku 86 , they appear to maintain overall genome integrity with no elevated p 53 levels or telomere shortening . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
Tumors , with increased DNA binding activity , also showed a statistically significant increase in Ku 70 and Ku 86 nuclear expression , as determined by Western blot and immunohistochemical analyses ( P < 0 . 001 ) . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
In addition , correlation studies between early pathological tumour response to radiation and expression of Ku 86 , Ku 70 , Mdm 2 , p 53 and p 21 in primary tumours were also performed . ^^^ We found that tumour transformed tissue shows positive immunostaining of DNA PKcs , Ku 86 and Ku 70 , while non neoplastic squamous epithelium and tumour free cervix glands show negative immunoreactivity . ^^^ Expression of DNA PKcs positively correlated with both Ku 86 and Ku 70 , and a statistically significant correlation between the Ku subunits was also found . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
Matrix assisted laser desorption ionization mass spectrometry ( MALDI MS ) of the tryptic digests of these proteins , identified one as the 86 kDA Lupus KU autoantigen protein P 86 and the second as the 70 kDa Lupus KU autoantigen protein P 70 . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
Genes essential for the end joining reaction include : XRCC 4 encoding a protein of unknown enzymatic function ; XRCC 5 and XRCC 6 encoding 86 and 70 kDa subunits of the Ku autoantigen , a DNA end binding protein that is also the regulatory subunit of DNA dependent protein kinase ( DNA PK ) ; and XRCC 7 encoding the catalytic subunit ( DNA PKcs ) of DNA PK . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
Microarray analysis revealed that DNA repair associated genes , i . e . , XRCC 5 , XRCC 6 , ERCC 5 , hMLH 1 were over expressed in three fold cisplatin resistant cell line , KFr as compared to cisplatin sensitive parental cell line , KF . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
Deficiency in nuclear accumulation of G22p1 and Xrcc 5 proteins in hyper radiosensitive Long Evans Cinnamon ( LEC ) rat cells after 10 irradiation . ^^^ DNA PK is a heterotrimeric protein complex comprised of two components : a large catalytic subunit , Prkdc , with serine / threonine kinase activity and a DNA targeting component , G22p1 and Xrcc 5 . ^^^ In previous report , we showed that approximately 80 % of the G22p1 and Xrcc 5 proteins were observed in the cytoplasm of rat fibroblasts , and that nuclear translocation of the proteins from the cytoplasm is important for the repair of DNA DSBs . ^^^ In the present study , we showed that nuclear accumulation of the G22p1 and Xrcc 5 proteins was not observed in fibroblasts from a mutant strain of Long Evans Cinnamon ( LEC ) rat that has an enhanced radiosensitivity and a reduced level of repair of DSBs after 10 irradiation . ^^^ Although high levels of G22p1 and Xrcc 5 proteins were observed in the nuclei of control rat cells until 60 min postirradiation , the amounts of the proteins decreased rapidly in the nuclei of LEC rat cells in the first 10 min after 10 irradiation . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
The presence of PRKDC , G22p1 and Xrcc 5 proteins was determined by Western blotting and a kinase assay used to measure DNA PK complex activity . ^^^
Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
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Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
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Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
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Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
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Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
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Interacting proteins: P13010 and P12956	Pubmed	SVM Score :0.0
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