| Interacting proteins: Q9UN19 and P12931 |
Pubmed |
SVM Score :0.0 |
| We have identified and characterized a novel src homology 2 ( SH 2 ) and pleckstrin homology ( PH ) domain containing adaptor protein , designated Bam 32 ( for B cell adaptor molecule of 32 kD ) . cDNAs encoding the human and mouse Bam 32 coding sequences were isolated and the human bam 32 gene was mapped to chromosome 4q25 q 27 . ^^^ |
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| Interacting proteins: Q9UN19 and P12931 |
Pubmed |
SVM Score :0.0 |
| This protein contains an amino terminal Src homology 2 ( SH 2 ) domain and a carboxy terminal pleckstrin homology ( PH ) domain , and is identical to the recently described DAPP 1 ( also known as PHISH or Bam 32 ) protein . ^^^ |
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| Interacting proteins: Q9UN19 and P12931 |
Pubmed |
SVM Score :0.0 |
| This differential regulation can be accounted for by Src homology 2 containing inositol polyphosphate 5 phosphatase ( SHIP ) activity alone , as expression of membrane targeted SHIP completely abrogated Btk recruitment , but had no inhibitory effect on Bam 32 or TAPP 2 recruitment . ^^^ |
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| Interacting proteins: Q9UN19 and P12931 |
Pubmed |
SVM Score :0.0 |
| Bam 32 contains one Src homology 2 and one pleckstrin homology domain and is phosphorylated at a single site , tyrosine 139 . ^^^ The basal level of F actin increased in cells expressing wild type or myristoylated Bam 32 but decreased in cells expressing either an Src homology 2 or Tyr 139 Bam 32 mutant . ^^^ |
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| Interacting proteins: Q9UN19 and P12931 |
Pubmed |
SVM Score :0.0 |
| An inhibitor of Src family protein tyrosine kinases ( PTKs ) blocked both BCR induced tyrosine phosphorylation of Bam 32 and BCR internalization . ^^^ These data suggest that Bam 32 functions downstream of Src family PTKs to regulate BCR internalization . ^^^ Collectively , these findings indicate a novel role of Bam 32 in connecting Src family PTKs to BCR internalization by an actin dependent mechanism . . ^^^ |
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| Interacting proteins: Q9UN19 and P12931 |
Pubmed |
SVM Score :0.0 |
| We recently identified a novel adaptor protein , termed dual adaptor for phosphotyrosine and 3 phosphoinositides ( DAPP 1 ) , that possesses a Src homology ( SH 2 ) domain and a pleckstrin homology ( PH ) domain . ^^^ We demonstrated that Tyr ( 139 ) of DAPP 1 is likely to be phosphorylated in vivo by a Src family tyrosine kinase , since the specific Src family inhibitor , PP 2 , but not an inactive variant of this drug , PP 3 , prevented the agonist induced tyrosine phosphorylation of DAPP 1 . ^^^ Src , Lyn and Lck tyrosine kinases phosphorylate DAPP 1 at Tyr ( 139 ) in vitro at similar rates in the presence or absence of PtdIns ( 3 , 4 , 5 ) P ( 3 ) , and overexpression of these kinases in HEK 293 cells induces the phosphorylation of Tyr ( 139 ) . ^^^ These findings indicate that , following activation of PI 3 kinases , PtdIns ( 3 , 4 , 5 ) P ( 3 ) or PtdIns ( 3 , 4 ) P ( 2 ) bind to DAPP 1 , recruiting it to the plasma membrane where it becomes phosphorylated at Tyr ( 139 ) by a Src family tyrosine kinase . . ^^^ |
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| Interacting proteins: Q9UN19 and P12931 |
Pubmed |
SVM Score :0.0 |
| DAPP 1 ( dual adaptor for phosphotyrosine and 3 phosphoinositides 1 ) is a broadly distributed pleckstrin homology ( PH ) and Src homology 2 domain containing protein that can bind phosphatidylinositol 3 , 4 , 5 trisphosphate ( PtdIns ( 3 , 4 , 5 ) P ( 3 ) ) and can be phosphorylated on tyrosine 139 and internalised in response to activation of type 1 phosphoinositide 3 kinases ( PI3K ) . ^^^ Tyrosine phosphorylation of DAPP 1 appears important for appropriate intracellular targeting and creates a potential binding site for Src homology 2 domain containing proteins . ^^^ In endothelial cells overexpressing wild type platelet derived growth factor beta ( PDGFbeta ) receptors , which express Bmx and Src as their major Btk ( Bruton ' s tyrosine kinase ) family and Src family tyrosine kinases , respectively , PDGF can stimulate PI3K dependent tyrosine phosphorylation of DAPP 1 . ^^^ Transient overexpression of Src most effectively , compared with Bmx and Syk , augments basal and PDGF stimulated tyrosine phosphorylation of DAPP 1 , whereas overexpression of dominant negative Src , but not dominant negative Bmx , inhibits PDGF stimulated phosphorylation of DAPP 1 . ^^^ Cells expressing mutant PDGFbeta ( Y579F / Y581F ) receptors ( which fail to bind and activate Src type kinases ) fail to tyrosine phosphorylate DAPP 1 in response to PDGF . ^^^ |
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| Interacting proteins: Q9UN19 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q9UN19 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
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