| Co immunoprecipitation revealed an interaction of c Src with Dok 4 WT in endothelial cells . ^^^ Most interestingly , overexpression of Dok 4 WT , but not Dok 4 ( deltaN 1 99 ) , increased mitochondrial c Src expression , whereas knock down of endogenous Dok 4 with a small interfering RNA vector greatly inhibited mitochondrial localization of c Src , suggesting a unique function for Dok 4 as an anchoring protein for c Src in mitochondria . ^^^ In addition , Dok 4 induced NF kappaB activation was also inhibited by transfection of a dominant negative form of c Src . ^^^ These data suggest a role for mitochondrial Dok 4 as an anchoring molecule for the tyrosine kinase c Src , and in turn as a regulator of TNF alpha mediated ROS production and NF kappaB activation . . ^^^ Mitochondrial Dok 4 recruits Src kinase and regulates NF kappaB activation in endothelial cells . ^^^ |