| Interacting proteins: Q15746 and P12931 |
Pubmed |
SVM Score :0.0 |
| Immunoprecipitation of EC MLCK after DPV challenge revealed dramatic time dependent tyrosine phosphorylation of the kinase in association with increased MLCK activity and a stable association of MLCK with the p 85 actin binding protein cortactin and p 60 ( src ) . ^^^ These studies indicate that DPV activates the endothelial contractile apparatus in a Rho GTPase dependent fashion and suggests that p 60 ( src ) induced tyrosine phosphorylation of MLCK and cortactin may be important features of contractile complex assembly . . ^^^ |
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| Interacting proteins: Q15746 and P12931 |
Pubmed |
SVM Score :0.0 |
| Two high molecular weight MLCK splice variants , EC MLCK 1 and EC MLCK 2 ( 210 214 kDa ) , in human endothelium are identical except for a deleted single exon in MLCK 2 encoding a 69 amino acid stretch ( amino acids 436 505 ) that contains potentially important consensus sites for phosphorylation by p 60 ( Src ) kinase ( Lazar , V . , and Garcia , J . ^^^ However , only EC MLCK 1 is readily phosphorylated by purified p 60 ( Src ) in vitro , resulting in a 2 to 3 fold increase in EC MLCK 1 enzymatic activity ( compared with EC MLCK 2 and SM MLCK ) . ^^^ The sites of tyrosine phosphorylation catalyzed by p 60 ( Src ) are Tyr ( 464 ) and Tyr ( 471 ) within the 69 residue stretch deleted in the MLCK 2 splice variant . ^^^ These results demonstrate for the first time that p 60 ( Src ) mediated tyrosine phosphorylation represents an important mechanism for splice variant specific regulation of nonmuscle MLCK and vascular cell function . . ^^^ |
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| Interacting proteins: Q15746 and P12931 |
Pubmed |
SVM Score :0.0 |
| In corroboration , immunocytochemistry with antibodies to various kinases ( PKCmu , zeta , and gamma , AKAP 220 , CaMKII , MLCK , and Src ) showed increased staining around dying spermatocytes following EGME treatment in vivo . 2D PAGE , autoradiography , and nanospray mass spectrometry was used to separate and identify proteins whose phosphorylation status was most greatly changed following exposure to MAA . ^^^ |
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| Interacting proteins: Q15746 and P12931 |
Pubmed |
SVM Score :0.0 |
| SS induced cortactin translocation was independent of Erk 1 , 2 MAP kinase , p 60 ( Src ) , MLCK , or RhoK activities , and unaffected by overexpression of a cortactin mutant lacking four major p 60 ( Src ) phosphorylation sites . ^^^ |
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| Interacting proteins: Q15746 and P12931 |
Pubmed |
SVM Score :0.0 |
| We previously noted that the high molecular weight endothelial MLCK isoform ( EC MLCK ) is stably associated with a complex containing p 60 ( src ) and 80kDa cortactin , an actin binding protein and known p 60 ( src ) target . ^^^ Tyrosine phosphorylation of either EC MLCK ( Y ( 464 ) , Y ( 471 ) ) or cortactin ( Y ( 421 ) , Y ( 466 ) , and Y ( 482 ) ) by p 60 ( src ) significantly increased this direct association . ^^^ |
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| Interacting proteins: Q15746 and P12931 |
Pubmed |
SVM Score :0.0 |
| FAK Src signalling through paxillin , ERK and MLCK regulates adhesion disassembly . ^^^ Using these assays , we show that kinases and adaptor molecules , including focal adhesion kinase ( FAK ) , Src , p130CAS , paxillin , extracellular signal regulated kinase ( ERK ) and myosin light chain kinase ( MLCK ) are critical for adhesion turnover at the cell front , a process central to migration . . ^^^ |
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| Interacting proteins: Q15746 and P12931 |
Pubmed |
SVM Score :0.0 |
| Direct binding of EC MLCK to the cortactin Src homology 3 domain appears essential to S1P barrier regulation , since cortactin blocking peptide inhibits both S1P induced MLC phosphorylation and peak S1P induced TER values . ^^^ |
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| Interacting proteins: Q15746 and P12931 |
Pubmed |
SVM Score :0.0 |
| Inhibitors of MEK , ROCK , or MLCK also suppress peripheral accumulation of phospho myosin and Src induced formation of integrin dependent adhesions , whereas at the same time restoring E cadherin redistribution to regions of cell cell contact . ^^^ |
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| Interacting proteins: Q15746 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q15746 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15746 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
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