| Interacting proteins: Q15139 and P12931 |
Pubmed |
SVM Score :0.0 |
| We show that tyrosine phosphorylation of protein kinase D ( PKD ) at Y 463 in the Pleckstrin Homology ( PH ) domain is mediated by the Src and Abl tyrosine kinase signaling pathway , and that this is both necessary and sufficient to activate NF kappaB in response to oxidative stress . ^^^ |
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| Interacting proteins: Q15139 and P12931 |
Pubmed |
SVM Score :0.0 |
| Lipid raft disruption triggers protein kinase C and Src dependent protein kinase D activation and Kidins 220 phosphorylation in neuronal cells . ^^^ |
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| Interacting proteins: Q15139 and P12931 |
Pubmed |
SVM Score :0.0 |
| Genistein , or the specific Src inhibitors PP 1 and PP 2 ( 1 10 micrometer ) inhibited H ( 2 ) O ( 2 ) mediated PKD activation by 45 % , indicating that Src contributes to this signaling pathway . ^^^ PKD activation by H ( 2 ) O ( 2 ) was also selectively potentiated by cotransfection of PKD together with an active form of Src ( 5 Src ) in COS 7 cells , as compared with PDB mediated activation . ^^^ In contrast , PKC inhibitors blocked H ( 2 ) O ( 2 ) or PDB mediated PKD activation essentially completely , suggesting that whereas Src mediates part of its effects via phospholipase C activation , PKC acts more proximally as an upstream activator of PKD . ^^^ Together , these studies reveal that oxidative stress activates PKD by initiating distinct Src dependent and independent pathways involving PKC . . ^^^ |
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| Interacting proteins: Q15139 and P12931 |
Pubmed |
SVM Score :0.0 |
| PKD activation was not promoted by pervanadate ( an inhibitor of tyrosine phosphatases ) and was not blocked by PP 1 ( an inhibitor of Src family kinases ) or genistein ( a tyrosine kinase inhibitor ) , suggesting that tyrosine kinases and phosphatases did not play a major role in PKD activation . ^^^ |
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| Interacting proteins: Q15139 and P12931 |
Pubmed |
SVM Score :0.0 |
| Mutational analysis revealed three tyrosine phosphorylation sites ( Tyr ( 432 ) , Tyr ( 463 ) , and Tyr ( 502 ) ) , which are regulated by the Src Abl pathway , and phosphorylation of only one of these ( Tyr ( 463 ) ) leads to PKD activation . ^^^ |
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| Interacting proteins: Q15139 and P12931 |
Pubmed |
SVM Score :0.0 |
| We previously demonstrated that phosphorylation of PKD at Tyr 463 in the PH ( pleckstrin homology ) domain is mediated by the Src Abl pathway and that it is necessary for PKD activation and subsequent NF kappaB induction . ^^^ |
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| Interacting proteins: Q15139 and P12931 |
Pubmed |
SVM Score :0.0 |
| Although oxidative stress induced PKD activation loop phosphorylation and activation with identical kinetics , both were dose dependently blocked by preincubation of cells with selective inhibitors of PKC ( GF109203X and G6983 ) or c Src ( PP 2 ) . ^^^ Inhibition of Src tyrosine kinase activity eliminated oxidative stress induced direct PKD tyrosine phosphorylation , but only partially attenuated activation loop phosphorylation and activation . ^^^ |
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| Interacting proteins: Q15139 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q15139 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q15139 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15139 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15139 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
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