Pubmed abstracts for Protein-Protein Interaction search result :

Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.7024786
Moreover , PYK 2 formed a complex coprecipitable with catalytically active c Src after Ang 2 stimulation . 0.7024786^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
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Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
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Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
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Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
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Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
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Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
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Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
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Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
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Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
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Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
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Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
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Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
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Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
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Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
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Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
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Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
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Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
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Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
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Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
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Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
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Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
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Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
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Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
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Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
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Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
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Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
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Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
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Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
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Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
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Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
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Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
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Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
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Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
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Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
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Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
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Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
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Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
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Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
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Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
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Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
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Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
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Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
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Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
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Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
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Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
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Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
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Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
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Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
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Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.80067946
These data suggest that mechanical stretch stimulates activation of CAKbeta , followed by its association with c Src , which requires ion influx mainly via stretch activated nonselective ion channels , thereby leading to activation of the p 21 ( Ras ) / ERK1 / 2 cascade in vascular smooth muscle cells . . 0.80067946^^^ Mechanical stretch rapidly ( within 1 min ) increased the association of CAKbeta with c Src , but not pp 125 ( focal adhesion kinase ) . 0.68336418^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.51467102
These results suggest that PKC activation ( but not Ca2+ mobilization ) is involved in PYK 2 tyrosine phosphorylation and that PYK 2 associates with Src without PYK 2 tyrosine phosphorylation or p130Cas involvement in platelets . . 0.51467102^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.54398132
We also observed ET induced temporal associations of Pyk 2 with active c Src , followed by p130Cas with Pyk 2 , c Src , and Crk . 0.54398132^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.66324083
This study is the first demonstration that PLD 2 activation is implicated in Src dependent phosphorylation of Pyk 2 ( Tyr ( 580 ) and Tyr ( 881 ) ) by promoting the complex formation between Pyk 2 and activated Src in PC 12 cells exposed to H ( 2 ) O ( 2 ) , thereby resulting in activation of the survival signaling pathway PI3K / Akt / p70S6K . . 0.66324083^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.52815272
The tyrosine kinase PYK 2 was found to co associate with Src and the channel , but PP 1 pre treatment reduced this co association . 0.52815272^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
Src and proline rich tyrosine kinase 2 ( PYK 2 ) activities were measured by immunoprecipitation and [ r 32P ] incorporation . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
In our study , HBx activated cytosolic calcium dependent proline rich tyrosine kinase 2 ( Pyk 2 ) , a Src kinase activator . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
SHP 2 interacts with RAFTK through a region other than its Src homology 2 domains . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
Evidence suggests ligation of the TCR induces activation of a PTK that may be a member of the src family . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
All these data indicated that purified PTK is closely related to pp60c src of human platelets . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
A 17 kb genomic clone encoding human p 56 Lck , a lymphocyte specific PTK of the Src related subfamily , has been isolated . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
Conversely , NMDA currents are potentiated by intracellular application of the well characterized PTK pp60c src . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
One IL 2 regulated PTK implicated in this cascade is the Src family kinase , Fyn . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
The regulation and biological function of the kinase activity of Src family PTK are not yet well established . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
At least one exogenous cytosolic PTK , pp60c src , is also able to potentiate NMDA current . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
Lysates of SW 2 and K 562 cells were analysed for expression of PTK of the c src family by Western blotting . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
CCK 8 enhanced protein tyrosine kinase ( PTK ) activities when the synthetic Src peptide was used as a substrate . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
In contrast , another Src family PTK , Fyn , was not affected by A6H ligation . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
These data suggest that CD 28 activates Tec via Src family PTK . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
This review summarizes our current knowledge on Lck , a member of the Src family of PTK , and its viral interaction partners . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
Protein tyrosine phosphorylation , Src PTK and p 38 expression and p 38 phosphorylation were examined by western blotting . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
Activation of Src family PTK activity at fertilization : role of the SH 2 domain . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
C terminal Src kinase ( Csk ) is a PTK that specifically phosphorylates Src family kinases on a C terminal Tyr . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
Convergence of Gi and Gq mediated pathways on calcium / calmodulin , Pyk 2 , and Src kinase . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
Here we provide evidence that p 55 is the Src family kinase Fyn and p 120 is the focal adhesion kinase family member Pyk 2 . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
However , there was no detectable activation of either JNK or c src , two other known downstream targets of Pyk 2 . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
By contrast , Cas mediated uptake in the absence of Fak requires Crk as well as the protein tyrosine kinases Pyk 2 and Src . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
Calcitonin ( CT ) induced changes in Pyk 2 , FAK , and Erk1 / 2 phosphorylation were independent of c Src . . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
Podosomes , alphaVbeta 3 receptor , c Src , and PYK 2 were unremarkable . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
PYK 2 has ligand sequences for Src homology 2 and 3 ( SH 2 and SH 3 ) , and has binding sites for paxillin and p 130 ( cas ) . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
Our data also indicate that the enhanced Pyk 2 level found in FAK / MEF plays no role in 5 Src induced anchorage independence . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
Greater than 85 90 % of Src and Fyn were present in lipid rafts , whereas Pyk 2 was mainly associated with PSDs . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
Pyk 2 activation was independent of G ( 1 ) and Src and was dependent on Rho . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
They had no direct inhibitory effect on the tyrosine kinases Src , Syk , or Pyk 2 . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
We also discuss the regulation of Src family kinases by PLD 1 , as related to the modulation of Pyk 2 and cell migration . . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
PKC depletion did not alter Ang 2 dependent tyrosine phosphorylation or activity of p 125 ( FAK ) , CADTK , Fyn or Src , but PKC depletion or incubation with GF109203X resulted in Ang 2 dependent EGF receptor tyrosine phosphorylation . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
Tyrosine kinases activated by AngII include c Src , focal adhesion kinase ( FAK ) , Pyk 2 ( CADTK ) , Janus kinases ( JAK 2 and TYK 2 ) , and the receptor tyrosine kinases Ax 1 , epidermal growth factor , and platelet derived growth factor . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
Mutation of Tyr 881 , a putative Src kinase phosphorylation site predicted to bind Grb 2 , had little effect on CADTK regulation . ^^^ Src family tyrosine kinases resulted in CADTK tyrosine phosphorylation even when co expressed with the Tyr402 / Tyr881 double mutant , suggesting that Src / Fyn etc . phosphorylate additional tyrosine residues . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
In vitro phosphorylation assays showed that PDGF receptor , calcium dependent tyrosine kinase ( CADTK / Pyk 2 ) , Src kinase , and epidermal growth factor receptor all were able to phosphorylate purified annexin 7 and 11 on tyrosine residues . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
A Galphaq pathway is responsible for this Src dependent PACAP enhancement because it was attenuated in mice lacking expression of phospholipase C beta 1 , it was blocked by preventing elevations in intracellular Ca2+ , and it was eliminated by inhibiting either PKC or cell adhesion kinase beta [ CAKbeta or Pyk 2 ( proline rich tyrosine kinase 2 ) ] . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
Pertussis toxin insensitive G proteins , most likely from G alpha 12 class , cause the activation of several cytoplasmic protein tyrosine kinases [ Src , Pyk 2 ( proline rich tyrosine kinase 2 ) , and Fak ( focal adhesion kinase ) ] . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
Whereas PSD associated proline rich tyrosine kinase 2 ( PYK 2 ) and gp145TrkB were elevated immediately after the ischemic event , increases in Src and Fyn were not apparent until 6 hours of reperfusion . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
AVP also stimulates the tyrosine phosphorylation of the nonreceptor tyrosine kinase proline rich tyrosine kinase 2 ( Pyk 2 ) and promotes Src family kinase phosphorylation at Tyr ( 418 ) , indicative of Src activation . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
Focal adhesion kinase ( FAK ) and the related proline rich tyrosine kinase 2 ( PYK 2 ) are non receptor protein tyrosine kinases that transduce extracellular signals through the activation of Src family kinases and are highly enriched in neurones . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
Internalisation of the human pathogen Yersinia pseudotuberculosis via interaction of bacterial invasin with host beta 1 integrins depends on the actin cytoskeleton and involves Src family kinases , focal adhesion kinase , p130Crk associated substrate , proline rich tyrosine kinase 2 , Rac , Arp 2 / 3 complex and WASP family members . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
Besides coupling with heterotrimeric G proteins to activate phospholipase C beta ( PLC beta ) , AT1R also activates receptor tyrosine kinases ( PDGF R , EGF R and IGF R ) and non receptor tyrosine kinases ( Src , Fyn , Yes , proline rich tyrosine kinase 2 ( Pyk 2 ) , focal adhesion kinase ( FAK ) and JAK 2 ) . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
Src homology 3 binding sites in the P2Y2 nucleotide receptor interact with Src and regulate activities of Src , proline rich tyrosine kinase 2 , and growth factor receptors . ^^^ A mutant P2Y ( 2 ) receptor lacking the PXXP motifs was found to stimulate calcium mobilization and serine / threonine phosphorylation of the Erk1 / 2 mitogen activated protein kinases , like the wild type receptor , but was defective in its ability to stimulate tyrosine phosphorylation of Src and Src dependent tyrosine phosphorylation of the proline rich tyrosine kinase 2 , epidermal growth factor receptor ( EGFR ) , and platelet derived growth factor receptor . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
Proline rich tyrosine kinase 2 and Src kinase signaling transduce monosodium urate crystal induced nitric oxide production and matrix metalloproteinase 3 expression in chondrocytes . ^^^ MSU induced functional signaling by specific protein kinases ( p 38 , Src , and the focal adhesion kinase [ FAK ] family members proline rich tyrosine kinase 2 [ Pyk 2 ] and FAK ) was also examined using selective pharmacologic inhibitors and transfection of kinase mutants . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
Our studies indicated that cerebral ischemia stimulated the rapid activation of nonreceptor tyrosine kinases proline rich tyrosine kinase 2 ( Pyk 2 ) and Src and the binding to Pyk 2 activated the latter . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
Given the important role for c Src in integrin mediated signal transduction , we have examined the potential regulation of BK ( Ca ) channels by proline rich tyrosine kinase 2 ( Pyk 2 ) , a calcium sensitive tyrosine kinase activated upon integrin stimulation . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
In contrast , phosphorylation of the extracellular signal regulated kinase ERK 2 , the ERK kinase MEK , proline rich tyrosine kinase 2 ( Pyk 2 ) , and Src family kinases was significantly , but transiently , increased . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
A new study in this issue shows that proline rich tyrosine kinase 2 ( Pyk 2 ) is responsible for acid induced activation of c Src and is essential for acid sensing in renal epithelial cells . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
IGF 1 increased tyrosine phosphorylation of the focal adhesion kinase ( FAK ) Pyk 2 ( calcium dependent proline rich tyrosine kinase 2 ) to a much greater extent than FAK , and increased association of Src with Pyk 2 but not FAK . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
The integrin ligand peptide Gly Arg Gly Asp Ser Pro ( GRGDSP ) induced rapid ( within 5 min ) and robust increases in tyrosine phosphorylation of focal adhesion kinase , proline rich tyrosine kinase 2 and Src family kinases . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
Using pharmacological and molecular approaches , we assessed the involvement of protein kinase C , Src kinases , and proline rich tyrosine kinase 2 in the process . ^^^ The broad spectrum protein kinase C inhibitor and the Src kinase inhibitor both inhibited tumor necrosis factor alpha induced proline rich tyrosine kinase 2 phosphorylation , but at different tyrosine residues . ^^^ Tumor necrosis factor alpha caused disorganization of the actin cytoskeleton by a mechanism dependent on protein kinase C , Src kinases , and proline rich tyrosine kinase 2 . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
The latter protein was identified by MALDI TOF / TOF MS as the proline rich tyrosine kinase 2 ( Pyk 2 ) that co operates with Src in a PLD 2 dependent manner . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
Analysis of tyrosine phosphorylated RAFTK from adherent transfected COS cells showed that the Src homology 2 ( SH 2 ) domains of the Src and Fyn protein kinases as well as the Grb 2 adaptor protein were able to specifically associate with RAFTK . ^^^ These data suggest that RAFTK is a novel member of the FAK family , that it localizes to focal adhesion like structures in CMK megakaryocytic cells , that it participates in integrinmediated signaling pathways in megakaryocytes , and that it is able to associate with the tyrosine kinases Src and Fyn as well as the adaptor protein Grb 2 via SH 2 phosphotyrosine interactions . . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
RAFTK has an overall 48 % amino acid homology to p 125 ( FAK ) and contains a kinase domain but lacks a transmembrane region , myristylation sites , and Src homology region 2 and 3 domains . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
The structure of RAFTK is similar to p125FAK in that it lacks a transmembrane region , does not contain Src homology 2 or 3 domains , and has a proline rich region in its C terminus . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
Furthermore , Tyr 402 , which mediates the binding of RAFTK to c Src kinase , was required for the phosphorylation of the C terminal domain of p 130 ( Cas ) . ^^^ These data suggest that RAFTK itself is sufficient for HEF 1 phosphorylation , whereas a cooperation between RAFTK and Src kinases is required for the complete phosphorylation of p 130 ( Cas ) . . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
Furthermore , in contrast to the phosphatase dead SHPTP 1 C453S mutant , overexpression of wild type SHPTP 1 blocks interaction of RAFTK with the SH 2 domain of c Src and inhibits RAFTK mediated MAPK activation . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
Expression of wild type RAFTK enhanced GPCR mediated JNK / SAPK activation , whereas dominant negative mutant constructs of RAFTK , such as K457A ( which lacks kinase activity ) and Y402F ( a Src binding mutant ) , inhibited KSHV GPCR mediated activation of JNK / SAPK . ^^^ RAFTK also mediated the KSHV GPCR induced activation of Lyn , a Src family kinase . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
Analyses of the members of the HRG stimulated complex revealed that RAFTK is associated with p 190 RhoGAP ( p 190 ) , RasGAP and ErbB 2 , and plays an essential role in mediating the tyrosine phosphorylation of p 190 by Src . ^^^ Mutation of the Src binding site within RAFTK ( 402 ) abolished the phosphorylation of p 190 . ^^^ In addition , upon HRG stimulation of T47D cells , association of ErbB 2 with RAFTK was observed and found to be indirect and mediated by Src . ^^^ Expression of wild type RAFTK ( WT ) significantly increased MDA MB 435 and MCF 7 breast cancer cell invasion , while expression of the kinase mutated RAFTK R 457 ( KM ) or the Src binding site mutant RAFTK ( 402 ) did not affect this cell invasion . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
Since activation of RAFTK is associated with the activity of Src family kinases , we analyzed whether CHK is capable of opposing HRG induced activation of RAFTK . ^^^ This interaction was mediated through the Src binding site ( amino acid residue at 402 ) of RAFTK and the SH 2 domain of CHK . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
PRAP was found to function as a substrate for Src family kinases , such as c Src or Fyn , but not for Pyk2 / RAFTK . ^^^ Pyk2 / related adhesion focal tyrosine kinase ( RAFTK ) tyrosine kinase is an upstream regulator of Src family kinases in the central nervous system that is involved in alpha synuclein phosphorylation . ^^^ Hyperosmotic stress induced phosphorylation of tyrosine 125 of alpha synuclein via Pyk2 / RAFTK , which acted through Src family kinases . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
RAFTK / Pyk2 activation is mediated by trans acting autophosphorylation in a Src independent manner . ^^^ Furthermore , RAFTK autophosphorylation occurred only at site Tyr 402 in a Src kinase activity independent manner . ^^^ However , Src significantly enhanced RAFTK mediated paxillin phosphorylation , suggesting a key role for Src in RAFTK activation and phosphorylation of downstream substrates . ^^^ Second , phosphorylated Tyr 402 recruits and activates Src kinase that in turn phosphorylates RAFTK and enhances its kinase activity . ^^^ Taken together , these studies provide insights into the molecular mechanism of RAFTK autophosphorylation and the specific role of Src in the regulation of RAFTK activation . . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
Cardiomyocyte apoptosis triggered by RAFTK / pyk2 via Src kinase is antagonized by paxillin . ^^^ Adenoviral mediated expression of RAFTK in neonatal rat cardiomyocytes involves concurrent increases in phosphorylation of Src , c Jun N terminal kinase , and p 38 leading to characteristic apoptotic changes including cleavage of poly ( ADP ribose ) polymerase , caspase 3 activation , and increased DNA laddering . ^^^ DNA laddering was decreased by mutation of the Tyr ( 402 ) Src binding site in RAFTK , suggesting a central role for Src activity in apoptotic cell death that was confirmed by adenoviral mediated Src expression . ^^^ Multiple apoptotic signaling cascades are recruited by RAFTK as demonstrated by prevention of apoptosis using caspase 3 inhibitor 4 ( caspase 3 specific inhibitor ) , PP 2 ( Src specific kinase inhibitor ) , or Csk ( cellular negative regulator for Src ) , as well as dominant negative constructs for p38beta or MKP 1 . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
We studied five downstream effectors of growth factor receptors Ras , Raf , Src , phosphoinositide 3 kinase ( PI 3 kinase ) , and Akt ( PKB ) for their abilities to block apoptosis . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
TRANCE , a TNF family member , activates Akt / PKB through a signaling complex involving TRAF 6 and c Src . ^^^ Here , we demonstrate that TRANCE activates the antiapoptotic serine / threonine kinase Akt / PKB through a signaling complex involving c Src and TRAF 6 . ^^^ A deficiency in c Src or addition of Src family kinase inhibitors blocks TRANCE mediated PKB activation in osteoclasts . c Src and TRAF 6 interact with each other and with TRANCE R upon receptor engagement . ^^^ These results define a mechanism by which TRANCE activates Src family kinases and PKB and provide evidence of cross talk between TRAF proteins and Src family kinases . . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
The transforming effect of PI3K is supposed to occur on the basis of complex alterations in cellular signaling pathways : appearance of constitutively generated PI3K dependent mitogen signal and activation of some protooncogenes ( src , ras , rac , etc . ) , PI3K / PKB pathway stimulation resulting in delay of apoptosis and increase of cell survival , and actin cytoskeleton reorganization . . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
Src kinases regulate PKB activation and modulate cytokine and chemoattractant controlled neutrophil functioning . ^^^ Taken together , our results demonstrate a critical role for Src kinases in regulating neutrophil cytotoxic effector functioning through PI3K PKB . . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
We report that myr PKB renders proliferative responses of thymocytes more sensitive to TCR signals by increased and sustained activation of Src kinase Lck and the extracellular signal regulated kinase / mitogen activated protein kinase pathway . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
SDF 1alpha also activated Src family kinases ( SFKs ) , and its inhibitor PP 1 diminished the SDF 1alpha induced chemotaxis and activation of both ERK1 / 2 and Akt / PKB . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
This data driven , multivariate ( 16 conditions 10 31 components 10 3 time points = approximately 1 , 500 values ) proteomic approach identified a set of signaling network components most critically associated ( positively or negatively ) with differentiation ( Stat 3 , Raf 1 , MEK , and ERK ) , proliferation of undifferentiated cells ( MEK and ERK ) , and proliferation of differentiated cells ( PKB alpha , Stat 3 , Src , and PKC epsilon ) . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
Inhibition of insulin induced swelling by furosemide largely abolished activation of beta ( 1 ) integrin and phosphorylation of Src , but not of PKB . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
Inhibiting Src family tyrosine kinase activity blocks glutamate signalling to ERK1 / 2 and Akt / PKB but not JNK in cultured striatal neurones . ^^^ Exposing neurones to the Src family tyrosine kinase inhibitor PP 2 , but not the inactive analogue PP 3 , inhibited NMDA receptor induced phosphorylation of ERK1 / 2 and Akt / PKB in a concentration dependent manner , and reduced cAMP response element binding protein ( CREB ) phosphorylation . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
Activation of both PKB and Erk as well as survival in low serum containing media are all rescued by reexpression of Grb 2 containing mutations within the N terminal Src homology 3 ( SH 3 ) domain , but not by C terminal SH 3 domain mutants . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
Adenosine , CPA and Cl IB MECA mediated PKB phosphorylation were inhibited by pertussis toxin ( PTX blocks G ( 1 ) / G ( o ) protein ) , genistein ( tyrosine kinase inhibitor ) , PP 2 ( Src tyrosine kinase inhibitor ) and by the epidermal growth factor ( EGF ) receptor tyrosine kinase inhibitor AG 1478 . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
The PKB Y 315 residue , which is known to be phosphorylated by Src tyrosine kinase , was also a major site of phosphorylation by RET / PTC . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
H2O2 induced phosphorylation of ERK1 / 2 and PKB requires tyrosine kinase activity of insulin receptor and c Src . ^^^ However , pharmacological inhibition of Src family PTK by 4 amino 5 ( 4 chlorophenyl ) 7 ( tert butyl ) pyrazolo [ 3 , 4 d ] pyrimidine ( PP 2 ) almost completely blocked H2O2 stimulated phosphorylation of the p 85 subunit of PI3K , ERK1 / 2 , and PKB . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
Here we report that PGE ( 2 ) induces the association of a prostaglandin E receptor 4 / beta arrestin 1 / c Src signaling complex resulting in the transactivation of the EGFR and downstream Akt ( PKB ) signaling . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
Immunoprecipitation experiments using various src antibodies revealed that pp60c src contributed partially to the PTK activity detected in GCGPs , and that it is associated with several proteins of Mr 140 K , 120 K , 85 K and 50 K . ^^^ These studies show that pp60c src associated with GCGPs is an active PTK that could be involved in neuronal growth and development , transmembrane signalling , and in recognition and / or adhesive events . . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
The expression of the PTK encoding oncogenes fes , abl and src , was investigated with the use of antibodies generated to their respective gene products . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
Nonreceptor PTK encoded by mammalian proto oncogenes ( c src , fyn , c yes , c abl ) and their homologs in Drosophila exhibit patterns of expression and activities that suggest functions in axonal or dendritic outgrowth . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
In parallel with the changes in IL 2R beta expression , the SRC like protein tyrosine kinase ( PTK ) p 56 LCK could not be detected in IL 3 dependent cells , but was abundant in the IL 2 dependent cells and underwent markedly increased autophosphorylation in response to IL 2 . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
Using antibodies against a number of known oncogene products , we could determine that at least 70 % of the PTK activity in the cytosol originated from the presence of the c src oncogene product . ^^^ Furthermore , using the MCF 7 breast cancer cell line , it could be shown that the antibody against c src also precipitated a part of the cytosolic PTK activity . ^^^ Inasmuch as the cytosolic PTK activity parallels the malignancy in breast tumors ( Hennipman et al . , Cancer Res . , 49 : 516 521 , 1989 ) , and the majority of this activity is precipitated by anti Src antibodies , the c src protooncogene may play a key role in the manifestation of breast cancer . . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
Neither beta nor gamma subunit of Fc epsilon RI nor src family PTK was coprecipitated with the PLC gamma 1 anti PLC gamma 1 complexes . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
In this study , we have demonstrated that stress stimuli enhance protein tyrosine phosphorylation ( PTyr P ) , modulate protein tyrosine phosphatase ( PTPase ) activity , activate the src family protein tyrosine kinase ( PTK ) , p56lck , and enhance glucose uptake in human peripheral blood mononuclear cells . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
Since none of the seven TcR CD 3 subunits exhibits a recognizable kinase domain , it seems likely that the receptor complex is associated with an intracellular PTK . p59fyn and the T lymphocyte specific p56lck are two intracellular , non receptor , cell membrane associated PTK of the src family expressed in T lymphocytes . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
Human HCK , C SRC ( encoding p 60 Src PTK ) , C FGR and LCK ( encoding p 56 Lck , PTK ) genes thus share very similar exon / intron structures for the conserved exons . ^^^ The genomic locus of the human hemopoietic specific cell protein tyrosine kinase ( PTK ) encoding gene ( HCK ) confirms conservation of exon intron structure among human PTKs of the src family . ^^^ To date , the complete genomic structure of human src family members is only available for the C FGR gene ( encoding p 55 Fgr , PTK ) . ^^^ Sequence analysis and characterization of the intron / exon organization of the human HCK gene , encoding a hemopoietic specific cell PTK of the src related family , revealed a length of over 16 kb for the seven 3 ' exons . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
Protein tyrosine kinases ( PTK ) structurally related to the product of the c src protooncogene are differentially expressed in distinct hematopoietic differentiation lineages and may participate in specific signal transduction pathways . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
The PTK is the human analogue of the murine pp56LSTRA ( pp56lck ) and has significant homology with c src , c yes , and other members of the src family . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
In this study , a 10 fold elevated activity of PTK encoded by fyn , another src family gene , was demonstrated in CD 4 CD8 T cells in mutant mice . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
Unlike growth factor receptors , the T cell and B cell receptors lack intrinsic PTK activity but associate with several non receptor PTKs of the Src and Syk families . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
This phosphorylation leads to the recruitment of a second cytoplasmic PTK , ZAP 70 , through both of the ZAP 70 Src homology 2 domains and its phosphorylation . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
We propose that p72syk may act as an immediate receptor activated kinase upstream of the related p70zap PTK and the src family PTKs p56lck and p59fyn in T cells and that these src family PTKs act as signal amplifiers . . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
Src homology 2 ( SH 2 ) domains participate in protein tyrosine kinase ( PTK ) mediated cellular signal transduction through their ability to bind with high affinity to phosphotyrosyl ( pTyr ) bearing protein sequences . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
Mapping of the requirement for partitioning into caveolae demonstrated that the amino terminal sequence Met Gly Cys is both necessary and sufficient within the context of a Src family PTK to confer localization into caveolae . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
We show that invasion of HeLa cells by S . flexneri induces tyrosine phosphorylation of cortactin , a host cell protein previously identified as a cytoskeleton associated protein tyrosine kinase ( PTK ) substrate for the proto oncoprotein pp60c src . ^^^ The proto oncoprotein pp60c src appears to mediate tyrosine phosphorylation of cortactin , since overexpression of this PTK in HeLa cells specifically increases the level of cortactin tyrosine phosphorylation induced during bacterial entry . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
In keratinocytes , various growth factor receptors and cytosolic proteins , including the EGF and IGF receptors , the proteins of the src family and others , exhibit PTK activity . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
CSK is a predominantly cytosolic protein tyrosine kinase ( PTK ) that negatively regulates Src family PTKs by phosphorylation of a conserved tyrosine near their C termini . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
Although several lines of evidence suggest involvement of protein tyrosine kinase ( PTK ) activity in this process , the role of src family PTK p561ck ( lck ) is not known . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
The c src gene family has nine known members ( blk , c fgr , fyn , hck , lck , lyn , c src , c yes and yrk ) , each encoding a cytoplasmic protein tyrosine kinase ( PTK ) believed to be involved in signal transduction . ^^^ The c src PTKs also associated with transmembrane proteins that lack PTK activity , frequently by means of interactions involving their unique amino terminal sequences . . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
Using a src family protein tyrosine kinase ( PTK ) inhibitor , herbimycin A , the cell proliferation induced by the anti CD 6 mAb , IOR T 1 , in TPA treated T cells were effectively abolished . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
Since N myristoylation of Fyn PTK , a member of the Src family , has been shown to be a prerequisite for palmitolylation , a new single plasmid vector that allows overexpression of myristoylated Fyn substrate in Escherichia coli was developed . ^^^ It is likely that palmitoylation of Fyn and other Src family members by PAT regulates PTK localization and signaling functions . . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
We further demonstrated that ET 1 stimulated autophosphorylation of pp60c src and pp60c src catalyzed phosphorylation of a peptide substrate specific for PTK activity . ^^^ Thus , cross talk between G protein coupled receptors and nonreceptor PTK such as pp60c src might be involved in transcriptional regulation and mitogenic signaling by ET 1 . . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
High affinity binding was also observed with recombinant SH 2 domains from 5 src and 5 fps , raising the possibility of protein protein interactions between various members of the cytoplasmic PTK family . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
In B cell precursors ( BCP ) lacking a functional ARC , CD 19 is physically and functionally associated with Src family protein tyrosine kinases ( PTK ) . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
These data suggest that the process of duplication which generated the Src family of PTK is an ongoing process and provide an insight into the molecular evolution of this group of genes . . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
The activity of baculoviral expressed ZAP 70 was up regulated 10 fold when ZAP 70 was co infected and phosphorylated by the src family PTK , lck . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
We demonstrate here that LFA 1 is physically associated in the YT human NK like cell line cells with a PTK ( s ) that is distinct from Src family PTKs such as Lck , Fyn , or Lyn . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
The effect of GM CSF and M CSF on the activation of fyn kinase , a 59 kDa src family related protein tyrosine kinase ( PTK ) , was studied . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
The kinase activity of the Src family of PTKs is negatively regulated by phosphorylation at the C terminal tyrosine residue , and the PTK Csk has been demonstrated to phosphorylate this C terminal residue of the Src family of PTKs . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
Paxillin is tyrosine phosphorylated during interphase of the cell cycle by protein tyrosine kinases ( PTK ) such as c Src and Csk , and becomes dephosphorylated during mitosis . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
Here we show that adhesion of murine NIH3T3 fibroblasts to fibronectin promotes SH 2 domain mediated association of the GRB 2 adaptor protein and the c Src protein tyrosine kinase ( PTK ) with FAK in vivo , and also results in activation of mitogen activated protein kinase ( MAPK ) . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
Among the 13 distinct PTK catalytic domains identified in E 5 cells , two were novel : they were encoded by eteck , a member of the eph sub family of PTKs , and thy , a member of the src sub family of PTKs . . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
Tyrosine kinase assays of inhibitor treated brains demonstrated that at doses at which retinal axon extension was severely impaired , PTK activity , including that of src family proteins , was reduced by 50 60 % . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
We showed previously that the c Src nonreceptor PTK and the Grb 2 SH2 / SH3 adaptor protein bound directly to FAK after fibronectin stimulation ( D . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
In contrast , Shc , phosphatidylinositol 3 kinase ( Pl3K ) , Grb 2 , and Ras GTPase activating protein ( GAP ) bound with different affinities to the bis or monophosphorylated peptides , while the Src family protein tyrosine kinase ( PTK ) Fyn did not bind specifically to any of the tested peptides . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
Although IL2R beta and gamma chains lack an intrinsic protein tyrosine kinase ( PTK ) domain , these chains recruit various non receptor type PTKs , such as p56lck ( and other Src family PTKs ) , Jak PTKs and Syk PTKs . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
Taken together , these results demonstrate that ET 1 stimulates nonreceptor PTK activity , including pp60c src , by activating Ca2+ channels and subsequent influx of extracellular Ca2+ . . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
One of the earliest steps in the activation pathway is thought to involve the Src family PTKs , p 56 ( lck ) ( Lck ) and p 59 ( fyn ) ( Fyn ) ; however , the precise contribution of each PTK in TCR mediated signaling remains incompletely understood . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
Using a somatic genetic model , we first define a requirement for the Src family protein tyrosine kinase ( PTK ) Lck in mediating KIR tyrosine phosphorylation . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
Activation of rat mast cells through the receptor with high affinity for IgE ( Fc epsilonRI ) requires a complex set of interactions involving transmembrane subunits of the Fc epsilonRI and two classes of nonreceptor protein tyrosine kinase ( PTK ) . the Src family PTK p53 / p56 ( lyn ) ( Lyn ) and the Syk / ZAP family PTK p 72 ( syk ) ( Syk ) . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
In this study , we investigated if this treatment increases the level and distribution of prostatic PTK activity , and more specifically , if it alters the expression and / or activity of the Src family members p60src and p53 / 56lyn . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
The catalytic activity of src family protein tyrosine kinases ( src PTK ) is suppressed when a C terminal tyrosine is phosphorylated by an intracellular PTK , C terminal Src kinase ( Csk ) . ^^^ Csk overexpression resulted primarily in a down regulation of Fgr activity , an src PTK expressed in J774A . 1 , and hyperphosphorylation of several cellular proteins of 35 , 57 , 66 , 97 and 120 130 kDa . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
Induction of antisense src RNA expression diminished the amounts of c Src and its protein tyrosine kinase ( PTK ) activity in U 937 ASRC cells . ^^^ The declines in c Src and its PTK activity subsequently reduced the proliferation of U 937 ASRC cells . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
FN stimulated c Src PTK activity was enhanced by wild type FAK expression , whereas FN stimulated activation of ERK 2 was blocked by expression of the c Src binding site Phe 397 mutant of FAK . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
Stimulation of antigen receptors in T and B cells leads to the activation of the Src and Syk families of protein tyrosine kinases ( PTK ) . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
We recently reported the identification of GIYWHHY as an efficient and specific substrate for p 60 ( c src ) protein tyrosine kinase ( PTK ) by screening a secondary random peptide library ( Q . ^^^ Because both YIYGSFK and GIYWHHY are efficient and specific substrates for p 60 ( c src ) PTK , chimeric branched peptides based on these two sequences were synthesized . ^^^ These branched peptides inhibit p 60 ( c src ) PTK with high potency , indicating that the enzyme active site of p 60 ( c src ) PTK can accommodate more than a linear motif . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
Co precipitation studies have shown that Src related PTK , such as Lyn , associates with the p 85 regulatory subunit of PI 3 kinase via SH 2 and SH 3 domain binding with their cognate ligands . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
In contrast , intracellular application of the PTK pp 60 ( c src ) ( 30 U / ml ) resulted in a progressive increase in current amplitude , and this potentiation was prevented by pretreatment of the neurons with genistein . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
One of them , soluble tyrosine kinase 1 ( STK 1 ) , represents a soluble form of the c Src protein , which is apparently underphosphorylated on its C terminal tyrosine residue whereas the other ( STK 2 ) is a 48 kDa protein tyrosine kinase ( PTK ) , which is molecularly and functionally related to the C terminal Src kinase ( Csk ) . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
These findings indicate that activation of gp 60 stimulates the Src PTK signaling pathway , and thus regulates the transcytosis of albumin across the endothelial cell monolayer . . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
A variety of protein tyrosine kinase ( PTK ) inhibitors , genistein ( 20 microg / ml ) , herbimycin A ( 3 . 4 microM ) , and a specific pp 60 ( src ) peptide inhibitor ( 21 microM ) also significantly reduced , but did not entirely prevent , stretch induced VPF protein secretion ( respectively 63 % , 80 % , and 75 % inhibition ; P < 0 . 05 for all ) . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
By using co immunoprecipitation techniques and in vitro kinase assays , the uPAR associated proteins were identified as Janus ( Jak ) and Src non receptor protein tyrosine kinases ( PTK ) Jak 1 , Tyk 2 , and p 59 ( fyn ) , p53 / 56 ( lyn ) , p53 / 59 ( hck ) , and p 55 ( fgr ) . ^^^ Our studies therefore suggest that , in VSMC , the uPAR signaling complex utilizes at least two different mechanisms , a direct signaling pathway utilizing the Jak / Stat cascade and a second signal transduction mechanism via Src like protein tyrosine kinases . uPA induced signaling via Jak / Stat is most likely involved in the regulation of cell migration , while the functional purpose of the uPA associated Src PTK activation remains to be elucidated . . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
In this report , we show that the Src family nonreceptor protein tyrosine kinase ( PTK ) Lyn associates with aggregated IgA Fc receptor ( Fc alpha R ) in the monocytic cell line THP 1 . ^^^ The Src family PTK involved in Fc alpha R mediated tyrosine phosphorylation appears to be Lyn , which coprecipitated with aggregated Fc alpha R complexes . . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
T cell antigen receptor ( TCR ) engagement activates multiple protein tyrosine kinases ( PTKs ) , including the Src family member , Lck , and the Syk related PTK , ZAP 70 . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
Both involve host cell protein tyrosine kinase ( PTK ) activity , but they differ in that invasion requires Src family tyrosine kinases and calcium calmodulin activity . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
To examine which proteins are responsible for the elevated protein tyrosine kinase ( PTK ) activity in human head and neck squamous cell carcinoma ( HNSCC ) and adjacent histologically normal epithelium , paraffin embedded sections of these tissues were stained for PTK c Src . ^^^ Using double labeling techniques and antibodies against both the proliferation marker Ki 67 and PTK c Src , we have shown that c Src is overexpressed in areas of hyperproliferation in HNSCC , dysplastic epithelium , benign papillomas and inflamed normal tissue . ^^^ Our data indicate that c Src is ( one of ) the protein ( s ) responsible for the increased PTK activity in HNSCC . ^^^ We could not demonstrate that c Src expression is responsible for the increased PTK activity in normal epithelium adjacent to tumour tissue . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
The receptor ( R ) for epidermal growth factor ( EGF ) is expressed at high levels on human breast cancer cells and associates with ErbB 2 , ErbB 3 , and Src proto oncogene family protein tyrosine kinases ( PTKs ) to form membrane associated PTK complexes with pivotal signaling functions . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
Treatment of fibroblasts with protein kinase C inhibitors or with the PTK inhibitor herbimycin A or PP 1 resulted in reduced Src PTK activity , no Grb 2 binding to FAK , and lowered levels of ERK 2 activation . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
While the Src PTKs mediate the phosphorylation of TCR associated signaling subunits and the phosphorylation and activation of the Syk PTKs , the lack of a constitutively active Syk PTK has prohibited the analysis of Lck function downstream of these initiating signaling events . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
To investigate whether interferons ( IFNs ) selectively suppress the growth of solid tumor cells with elevated protein tyrosine kinase ( PTK ) activity , we evaluated the effect of recombinant IFN alpha2a and IFN gamma on the proliferative and neoplastic potentials triggered by p60v src using 5 src transformed HAG 1 human epithelial cells . ^^^ Pretreatment of cells with Src or the Src like PTK inhibitor herbimycin A or radicicol , alleviated dose dependently the growth inhibitory activity of IFN alpha2a against 5 src transformed cells , suggesting that IFNs may share a common inhibitory pathway with Src PTK inhibitors . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
On the basis of the efficient substrate for p60c src protein tyrosine kinase ( PTK ) YIYGSFK NH 2 ( 1 ) ( Km = 55 microM ) obtained by combinatorial methods , we have designed and synthesized a series of conformationally and topographically constrained substrate based peptide inhibitors of this enzyme , which showed IC 50 values in the low micromolar range ( 1 3 microM ) . ^^^ This substitution led to selective and potent inhibitors of p60c src PTK ; however , no substantial difference in potency was observed among them . ^^^ Two new potent iodo containing nonphosphorylatable tyrosine analogues were also incorporated into our lead inhibitory sequence 23 , producing the most potent inhibitors for p60c src PTK identified thus far in our studies . ^^^ Selectivity studies of peptides 23 30 toward p60c src , Lyn , and Lck PTK showed in general high Lyn / Src and moderate Lck / Src selectivity ratios . ^^^ We found that the chi 1 space constraints of the specialized amino acids , introduced at position 3 of the peptide lead 23 , were not as important as the configuration of the Calpha of that residue to recognize the subtle chemical environment surrounding the active site of Src and Lck PTK , as reflected on the obtained Lck / Src selectivity ratios . . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
Epidermal growth factor receptor ( EGFR ) in whole testis was measured , as were the activities of c Src kinase , protein tyrosine kinase ( PTK ) , mitogen activated protein 2 kinase ( MAP2K also termed as Erk 2 ) , protein kinase A ( PKA ) , and protein kinase C ( PKC ) . ^^^ Although TCDD significantly increased c Src kinase activity in immature and mature rats , opposite effects of TCDD on activities of PTK , PKA , and PKC were found in 34 day old rats vs 49 , 62 , and 90 day old rats . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
Upon platelet activation Lyn activity remains constant or diminishes and pp 60 ( c src ) PTK within this fraction becomes highly activated , indicating the dynamic nature of the membrane microdomains . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
Csk is a cellular protein tyrosine kinase ( PTK ) that has been shown to specifically regulate the activity of Src kinase family members by phosphorylation of a carboxy terminal tyrosine residue . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
Two broad specificity biotinylated PTK peptide substrates were identified with optimum kinetic properties , allowing members from eight distinct classes of enzymes , including transmembrane ( epidermal growth factor receptor ( EGFR ) , fibroblast growth factor receptor , insulin receptor , and platelet derived growth factor receptor ) and cytoplasmic ( p 43 ( abl ) , p 56 ( lck ) , p 60 ( src ) , and p 93 ( fes ) ) PTKs , to be analyzed . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
One focus of investigation was epidermal growth factor receptor ( EGFR ) and the key cytosolic signaling kinases , c Src and protein tyrosine kinase ( PTK ) , whose responses to TCDD are well characterized . ^^^ A marked increase in the protein amount of H Ras and a significant increase in the activity of c Src kinase , PTK , and Erk 2 were found in cells from TCDD treated animals . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
Consistent with an early role for the Src family PTKs in these signaling events , we found that transient activation of Hck and CD 59 mediated signaling was abrogated in the presence of the Src family PTK selective inhibitor PP 1 . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
In contrast , the A region , which mediates activation of the Src family protein tyrosine kinase ( PTK ) members , contributes to downregulation of the T cell proliferation function . ^^^ The IL 2Rbetac null mutant mice develop severe autoimmune symptoms ; these are all suppressed following the expression of either of the mutants , suggesting that neither the Stats nor the Src PTK members are required . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
Previously , we reported that another PTK termed p 50 ( csk ) was a potent negative regulator of T cell receptor ( TCR ) signaling because of its ability to inactivate Src related kinases . ^^^ In addition to giving novel insights into the mechanisms involved in the negative regulation of T cell activation , these findings indicate that the association of an inhibitory PTK with a PTP constitutes a more efficient means of inhibiting signal transduction by Src family kinases in vivo . . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
Fyn , a member of the Src family protein tyrosine kinase ( PTK ) , is implicated in learning and memory that involves N methyl D aspartate ( NMDA ) receptor function . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
Activation of Src , which has an intrinsic protein tyrosine kinase ( PTK ) activity , has been demonstrated in human solid tumors , such as colorectal and breast cancers . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
A close relationship between Sam 68 , a tyrosine and proline rich RNA binding protein , and Src protein tyrosine kinases ( PTK ) has already been established , also in T lymphocytes . ^^^ A constitutive phosphorylation of the molecule has also been documented in various transformed T cells , which probably reflects an increased expression of PTK of the Src family . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
Because Src related PTK have been shown to be activated in leukemic cell lines , we studied their activation in human myeloid leukemia . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
A parallel combinatorial library of over sixteen hundred compounds has been designed and synthesized for the development of new potential peptidomimetic protein tyrosine kinase ( PTK ) inhibitor leads that is aimed for intervening with the substrate binding site of the pp60c src enzyme . ^^^ Conformational analysis of several peptide substrates recently found for pp60c src PTK [ 5 , 6 , 7 ] showed that the energy minimized conformers had the same distance between two aromatic moieties . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
TRAIL gene expression was sensitive to the src like protein tyrosine kinase ( PTK ) inhibitor herbimycin A , as well as the more general PTK inhibitor genistein , suggesting the involvement of a src family PTK . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
Binding of pIgA to pIgR stimulates transcytosis of the pIgA pIgR complex via a signal transduction pathway that is dependent on a protein tyrosine kinase ( PTK ) of the SRC family . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
Of particular interest for this study is the ability of Nef to bind with a very high affinity to SH 3 domains of myelomonocyte specific protein tyrosine kinases of the Src family ( Src like PTK ) . ^^^ This effect was not observed in undifferentiated HL 60 cells or in CEM T lymphocytes and correlated with the differentiation induced up regulation of Src like PTK . ^^^ The Src like PTK p56 / 59 ( hck ) co immunoprecipitated with both Nef and with the inositol 1 , 4 , 5 trisphosphate receptor , providing a possible mechanistic link between the viral protein and intracellular Ca ( 2+ ) stores of the host cell . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
The proline , glutamic acid , serine and threonine enriched protein tyrosine phosphatase PEP , which is expressed primarily in hematopoietic cells , was recently discovered to be physically associated with the 50 kDa cytosolic protein tyrosine kinase ( PTK ) Csk , an important suppressor of Src family PTK , including Lck and Fyn in T cells . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
Even at very high concentrations ranging from 175 350 microM , this inhibitor did not affect the enzymatic activity of other PTKs , including the Janus kinases JAK 1 and JAK 3 , the Src family kinase HCK , the Tec family member Bruton ' s tyrosine kinase , SYK kinase , and the receptor family PTK insulin receptor kinase . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
Western blot analysis further shows that the level of cSrc , a nonreceptor type of PTK , is 261 % higher in the kidneys from rats on a K deficient diet than those on a high K diet . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
The transmembrane tyrosine phosphatase CD 45 regulates the activity of src family protein tyrosine kinases ( PTK ) and thereby influences the signaling via such receptors as T and B cell antigen receptors associated with these PTK . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
MAPK is downstream of PTK , Src , and Janus tyrosine kinase pathways but may not be downstream of phosphatidylinositol 3 kinase pathways . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
In the inside out configuration , bath application of the recombinant PTK pp60c src ( 75 U / ml ) inhibited the GABA activated chloride current , and the inhibitory effect of pp60c src was prevented by genistein ( 10 7 M ) . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
The effects of a range of structurally different inhibitors of protein tyrosine kinases ( PTK ) were examined on voltage operated calcium channel currents ( 1 ( Ba ) ) and pp 60 ( c src ) kinase ( c src ) activity in vitro . 2 . 1 ( Ba ) was measured in single myocytes isolated from rabbit ear artery by conventional whole cell voltage clamp techniques . ^^^ Bath application of tyrphostin 23 and genistein ( non selective PTK inhibitors ) , bistyrphostin ( a receptor PTK selective inhibitor ) and PP 1 ( a src family selective inhibitor ) inhibited 1 ( Ba ) in a concentration dependent manner over a range of test membrane potentials . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
Postsynaptic application of a peptide activator of the non receptor PTK Src ( Src peptide ) enhanced mIPSC amplitudes by 39 % in the presence of brain derived neurotrophic factor ( BDNF ) only ; neurotrophin 3 ( NT 3 ) was ineffective in this regard . ^^^ It is concluded that , whilst GABAA receptors are not constitutively regulated by endogenous PTK activity in PCs , co activation of TrkB by BDNF and Src by mGluR 1 is required to modulate GABAergic synapses in PCs . . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
The resulting large Kv1 . 3 current was eliminated by co transfecting the constitutively active PTK , 5 src , then rapidly restored by the PTK inhibitor , lavendustin A . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
We investigated whether these observations were related to defects in signal transduction related to protein tyrosine kinase ( PTK ) of the src and syk families . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
Treatment of confluent chicken embryo fibroblasts ( CEFs ) with trypsin results in a dose and time dependent increase in c Src protein tyrosine kinase ( PTK ) activity . ^^^ A similar , but less marked , increase in c Src PTK activity occurs upon incubation of CEFs in calcium free phosphate buffered saline , which also causes a decrease in cell substrate adhesion . ^^^ The increase in c Src PTK activity following disruption of cell substrate adhesion correlates with a decrease in the phosphorylation of c Src at the regulatory site , Tyr 527 . ^^^ The phosphotyrosine phosphatase inhibitor phenylarsine oxide blocks the increase in c Src PTK activity seen following treatment with trypsin and the morphological changes associated with the disruption of cell substrate adhesion . ^^^ Treatment of cells with cytochalasin D , which disrupts actin filaments but not cell substrate adhesion , causes only a slight increase in c Src PTK activity . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
Tyrosine phosphorylation occurs primarily on tyrosine 125 and was inhibited by PP 2 , a selective inhibitor of Src protein tyrosine kinase ( PTK ) family members at concentrations consistent with inhibition of Src function ( ) . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
We have used the two electrode voltage clamp technique and the patch clamp technique to investigate the regulation of ROMK 1 channels by protein tyrosine phosphatase ( PTP ) and protein tyrosine kinase ( PTK ) in oocytes coexpressing ROMK 1 and cSrc . ^^^ Moreover , application of herbimycin A , an inhibitor of PTK , increased K ( + ) current by 120 % and completely abolished the effect of PAO in oocytes coexpressing ROMK 1 and cSrc . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
The protein tyrosine kinase ( PTK ) Csk is a potent negative regulator of several signal transduction processes , as a consequence of its exquisite ability to inactivate Src related PTKs . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
Virulent organisms induced early PTK activation and the tyrosine phosphorylation of several endogenous substrates , including Hck and Lyn , two Src related kinases . ^^^ In addition , lavendustin A , a PTK inhibitor , and PP 1 , a Src kinase inhibitor , prevented C . burnetii induced cell protrusions and F actin reorganization . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
We are the first to establish two fucoidan mediated signaling pathways : PTK ( Src ) / Rac1 / PAK / JNK and PTK ( Src ) / Rac1 / PAK / p38 , but not PTK / phospholipase C gamma 1 / PKC / MEK1 / ERK , playing critical roles in proIL 1 / IL 1 regulation . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
The intracellular calcium chelator 1 , 2 bis ( 2 aminophenoxy ) ethane N , N , N ' , N ' tetraacetic acid ( 10 ( 6 ) M ) , protein kinase C ( PKC ) inhibitors bisindoylmaleimide 4 ( 10 ( 6 ) M ) and calphostin C ( 10 ( 7 ) M ) , protein tyrosine kinase ( PTK ) inhibitor genistein ( 10 ( 4 ) M ) , Src family kinase inhibitor PP 2 ( 2 . 5 10 10 ( 7 ) M ) , p42 / 44 mitogen activated protein kinase ( MAPK ) inhibitor PD 98059 ( 10 ( 5 ) M ) , and the epidermal growth factor ( EGF ) inhibitor AG 1478 all attenuated GFAT promoter activation by ANG 2 . ^^^ Promoter activation is calcium dependent and PKC dependent but also involves PTK signaling pathways including Src , the EGF receptor , and p42 / 44 MAPK . . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
The 5 HT2A receptor antagonist sarpogrelate ( 10 microM ) and its major metabolite M 1 ( 0 . 1 microM ) , pertussis toxin ( 10 ng / ml ) , Src family protein tyrosine kinase ( PTK ) inhibitor PP 2 ( 1 microM ) , protein kinase C ( PKC ) inhibitor Ro 31 8220 ( 0 . 1 microM ) and mitogen activated protein kinase ( MAPK ) kinase inhibitor PD 098059 ( 10 microM ) significantly inhibited the mitogenic effect of 5 HT and its interaction with MCP 1 . ^^^ The mitogenic effect of 5 HT may be mediated by the G protein Src family PTK PKC MAPK pathway . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
We have used Western blot to examine the expression of cSrc protein tyrosine kinase ( PTK ) and protein tyrosine phosphatase ( PTP ) 1D in the renal cortex , and the patch clamp technique to determine the role of PTK in mediating the effect of dietary K intake on the small conductance K ( SK ) channel in the cortical collecting duct ( CCD ) . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
Regulation is achieved by cross talk between Src family PTKs and CD 19 ; Lyn is essential for CD 19 phosphorylation , while CD 19 establishes an Src family PTK activation loop that amplifies kinase activity . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
We have previously demonstrated that the protein level of c Src , a nonreceptor type of protein tyrosine kinase ( PTK ) , was higher in the renal medulla from rats on a K deficient ( KD ) diet than that in rats on a high K ( HK ) diet ( Wang WH , Lerea KM , Chan M , and Giebisch G . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
HIV SUgp 120 ( HIV surface glycoprotein ) , T cell receptor ( TCR ) CD4+ and co receptors promote aggregation of these lipid `` rafts ' ' which concentrate the Src family tyrosine kinases SFKs ( PTK , Lyn , Fyn , Lck ) , GPI ( glycosyl phosphatidylinositol ) anchored proteins , and phosphatidylinositol kinases PI ( 3 ) K and PI ( 4 ) K , inducing cell signalling . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
Dephosphorylation of cell extracts resulted in decreased association of ADAM 15 with Src family PTK SH 3 domains , indicating that phosphorylation influences ADAM 15 interactions with its binding partners . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
The ubiquitous Src family protein tyrosine kinase ( PTK ) Fyn is not activated by these E 5 constructs , nor are focal adhesion kinase and endogenous receptor PTKs for insulin , epidermal growth factor , basic fibroblast growth factor and insulin like growth factor . ^^^ L26A E 5 does not transform SYF cells that are deficient for Src , Fyn and Yes , unless Src expression is reconstituted , and does not transform NIH3T3 cells in which Src PTK activity is maintained at a basal level by means of kinase defective K295R Src overexpression . . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
These results demonstrate that ( 1 ) ischemic PC upregulates cardiac COX 2 via PKC , Src PTK , and NF kappaB dependent signaling pathways , whereas generation of NO and ROS is not necessary , and ( 2 ) the activity of newly synthesized COX 2 following PC requires iNOS derived NO whereas iNOS activity is independent of COX 2 derived prostanoids , indicating that COX 2 is located downstream of iNOS in the protective pathway of late PC . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
Administration of the Src PTK inhibitor lavendustin A ( LD A ; 1 mg / kg 4 ) before the PC ischemia on day 1 ( group 3 , n = 7 ) failed to block the delayed protective effect against myocardial infarction 24 h later . ^^^ We conclude that , in conscious rabbits , Src PTK activity is necessary for the mediation of late PC protection against myocardial infarction on day 2 , but not for the initiation of this phenomenon on day 1 . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
When tested as inhibitor of the autophosphorylation of the enzyme and the phosphorylation of the protein tyrosine phosphatase SHP 1 by c Src , lauryl gallate was found to be a more potent inhibitor than other widely used protein tyrosine kinase ( PTK ) inhibitors such as genistein and herbimycin A . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
Using the human B cell line Ramos , we demonstrate that CD 19 and CD 20 are two HLA class 2 associated receptors that couple HLA class 2 to PTK signaling pathway where CD 20 appears to be amajor component of HLA class 2 mediated activation of Src kinases . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
In both B 1 and B 2 cells BCR cross linking induced a modest increase of CD 19 associated Lyn , a Src family protein tyrosine kinase ( PTK ) thought to be important for CD 19 phosphorylation . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
Two PCR based strategies were used to show that 21 different PTK genes are expressed by rat brain microglia : 5 receptor PTKs , 10 nonreceptor PTKs , and 6 members of the src family . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
Src family PTKs are known to be present in glycolipid enriched microdomains ( GEMs ) , also called lipid rafts , and to be negatively regulated by PTK Csk complexed to tyrosine phosphorylated transmembrane adapter protein PAG ( phosphoprotein associated with GEMs ) also called Cbp ( Csk binding protein ) . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
This property results from the ability of SAP to promote the selective recruitment and activation of FynT , a cytoplasmic Src related protein tyrosine kinase ( PTK ) . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
We used confocal microscopy , patch clamp , and biotin labeling techniques to examine the role of soluble N ethylmaleimide sensitive factor attachment protein receptor ( SNARE ) proteins in mediating the effect of inhibition of PTK on ROMK 1 trafficking in HEK 293 cells transfected with c Src and green fluorescent protein ( GFP ) ROMK 1 . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
In this study , we investigated the effects of protein tyrosine kinase ( PTK ) and protein tyrosine phosphatase ( PTP ) on the tyrosine phosphorylation of N methyl D aspartate receptor subunit 2A ( NR2A ) and the interactions among NR2A , postsynaptic density protein 95 ( PSD 95 ) , Fyn / Src after brain ischemia / reperfusion ( I / R ) . ^^^ The following results were observed : ( 1 ) the increase in tyrosine phosphorylation of NR2A induced by I / R was suppressed by genistein , an inhibitor of PTK , but was further enhanced by sodium orthovanadate , an inhibitor of PTP , which were administered to the SD rats 20 min before ischemia . ( 2 ) Importantly , genistein and sodium orthovanadate increased and decreased the interactions involving NR2A , PSD 95 , Fyn and Src , respectively . ^^^ These results demonstrated that PTK and PTP were involved in regulating tyrosine phosphorylation of NR2A through changing the interaction among NR2A , PSD 95 , Fyn / Src . . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
We demonstrate that in B cells the HLA DR activated protein kinase C ( PKC ) beta is required for HLA DR mediated death whereas the HLA DR activated Src family of PTK is redundant . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
Studies by immunoprecipitation revealed that Fer kinase associated with N cadherin , gamma catenin , p120ctn , c Src ( a putative PTK and the product of the transforming , sarcoma inducing gene of Rous sarcoma virus ) , Rab 8 ( a GTPase ) , actin , vimentin , but not E cadherin , afadin , nectin 3 , and integrin beta 1 , suggesting Fer kinase associates not only with the actin based cell cell AJ structures , such as the N cadherin / catenin complex ( but not the alpha6beta1 integrin / laminin and the afadin / nectin complex ) , but also with intermediate filament based cell cell desmosomes . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
Protein tyrosine kinases ( PTK ) of the Src family are thought to suppress K Cl cotransport ( KCC ) activity via negative regulation of protein phosphatases . ^^^ We have reported previously that deoxygenation of sickle cells stimulates KCC and activates Syk ( a Syk family PTK ) , but not Lyn ( an Src family PTK ) . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
Phosphorylation of a subunit of N methyl D aspartate ( NMDA ) receptor by protein tyrosine kinase ( PTK ) Src or Trk is known to enhance its channel activity . ^^^ We examined whether a spinally administered selective PTK inhibitor , lavendustin A , which has high affinity for Src and Trk tyrosine kinases , could influence the development and maintenance of inflammatory hyperalgesia or NMDA induced hyperalgesia . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
In addition , pharmacological and genetic interference with Src PTK function reduces bacterial internalization . ^^^ Importantly , Src PTK deficient cells are resistant to S . aureus invasion , demonstrating the essentiality of host Src PTKs in integrin mediated uptake of this pathogen . . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
Together , these findings indicate that both ERK and JNK are involved in the regulation of TNF mRNA expression , that p 38 is involved in the nucleocytoplasmic transport of TNF mRNA , and that a PTK ( protein tyrosine kinase ) , possibly a member of the src family , acts downstream of the P2X7 receptor to activate JNK and p 38 . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
We investigated signal transduction between receptor operated Ca ( 2+ ) influx ( ROCI ) and Src related nonreceptor protein tyrosine kinase ( PTK ) in rat pancreatic acini . ^^^ CCK and the Ca ( 2+ ) ionophore enhanced the Src related PTK activity , whereas the high affinity CCK A receptor agonists , fibroblast growth factor ( FGF ) , and the protein kinase C ( PKC ) activator had no or little effect . ^^^ The low affinity CCK A receptor regulates ROCI via mediation of Src related PTK and activates Src pathways to cause [ Ca ( 2+ ) ] ( o ) dependent pancreatic exocytosis . . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
Furthermore , the selective Src family PTK inhibitor PP 2 ( 5 microM ) stimulated 1 ( Cl . vol ) , mimicking genistein , whereas the selective EGFR ( ErbB 1 ) kinase inhibitor tyrphostin B 56 ( AG 556 , 25 microM ) reduced 1 ( Cl . vol ) , mimicking A 23 and A 25 . ^^^ Src and EGFR kinases , distinct soluble and receptor mediated PTK families , have opposing effects on 1 ( Cl . vol ) , and multiple target proteins are likely to be involved . . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
Lyn is the only member of the Src family expressed in DT 40 B cells , which provide a unique model to study the singular contribution of this protein tyrosine kinase ( PTK ) family to cell signaling . ^^^ Changes in GANP expression were also confirmed in Lyn deficient mice , suggesting that Lyn PTK has a unique function not compensated for by other Src kinases . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
Induction of Pyk 2 tyrosine phosphorylation by CXCL 8 is regulated by Src PTK activation , whereas it is unaffected by phosphatidylinositol 3 kinase activation . ^^^ Inhibition of Pyk 2 activation by PP 1 , a Src PTK inhibitor , is paralleled by the inhibition of CXCL 8 mediated neutrophil chemotaxis . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
We previously demonstrated that dietary K intake regulates the expression of Src family PTK , which plays an important role in controlling the expression of ROMK 1 in plasma membrane ( Wei Y , Bloom P , Lin D H , Gu RM , and Wang WH . ^^^ In the present study , we used the immunofluorescence staining technique to demonstrate the presence of c Src , a member of Src family PTK , in the thick ascending limb ( TAL ) and collecting duct . ^^^ We conclude that c Src is expressed in the TAL , CCD , and OMCD and that stimulation of PTK increases the ROMK channels in the intracellular compartment but decreases them in the apical / subapical membrane in the CCD . . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
BACKGROUND : The authors tested the hypotheses that protein kinase C ( PKC ) specific isoform translocation and Src protein tyrosine kinase ( PTK ) activation play important roles in isoflurane induced preconditioning in vivo . ^^^ CONCLUSIONS : Protein kinase C delta , PKC epsilon , and Src PTK mediate isoflurane induced preconditioning in the intact rat heart . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
Recent investigation on the functional coupling of FcepsilonRI to the intracellular signaling apparatus has provided paradigm altering insights on the importance and function of Src family protein tyrosine kinases ( Src PTK ) in mast cell activation . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
Conversely , food deprivation but not NMDA injection produced marked increases in apparent activity for Src PTKs and in the expression of Pyk 2 , an Src activating PTK . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
To determine whether protein tyrosine kinase ( PTK ) modulates 1 ( Cl , swell ) and to identify the PTK involved , we studied the effects of a broad spectrum PTK inhibitor ( genistein ) , selective inhibitors of Src ( PP 2 , a pyrazolopyrimidine ) and epidermal growth factor receptor ( EGFR ) kinase ( PD 153035 ) , and a protein tyrosine phosphatase ( PTP ) inhibitor ( orthovanadate ) . 1 ( Cl , swell ) evoked by hyposmotic swelling was increased 181 + / 17 % by 100 microM genistein , and the genistein induced current was blocked by the selective 1 ( Cl , swell ) blocker tamoxifen ( 10 microM ) . ^^^ The results indicate that tyrosine phosphorylation controls 1 ( Cl , swell ) , and regulation of 1 ( Cl , swell ) by the Src and EGFR kinase families of PTK is antagonistic . . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
Prevention of noise induced hearing loss with Src PTK inhibitors . ^^^ The Src protein tyrosine kinase ( PTK ) signaling cascade may be involved in both metabolic and mechanically induced initiation of apoptosis in sensory cells of the cochlea . ^^^ The current study compares three Src PTK inhibitors , KX 1 004 , KX 1 005 and KX 1 174 as potential protective drugs for NIHL . ^^^ For all conditions , the results suggest a role for Src PTK activation in noise induced hearing loss ( NIHL ) , and that therapeutic intervention with a Src PTK inhibitor may offer a novel approach in the treatment of NIHL . . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
Moreover , Western blot analysis revealed that DOCA treatment significantly increased the expression of the nonreceptor type of protein tyrosine kinase ( PTK ) , cSrc , and the tyrosine phosphorylation of ROMK in the renal cortex and outer medulla . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
Calcitonin and alendondrate , significantly reduced resorption activity and PTP oc expression , and increased c Src PY 527 with corresponding reduction in its PTK activity . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
There was no significant change in total abundance of key renal K ( + ) transporters , but 50 % increases in both renal PTK cSrc abundance and ROMK phosphorylation in the 0 . 33 % K ( + ) vs . 1 % K ( + ) diet group , previously established to be associated with internalization of ROMK . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
The effect of genistein on the channel was also not affected by the presence of catalytically active PTK , p 60 ( c src ) inside the cell . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
By use of cellular and molecular approaches and transgenic animals , Src protein tyrosine kinase ( PTK ) family members have been identified to be essential for the recruitment and activation of monocytes , macrophages , neutrophils , and other immune cells . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
Indeed , in PC 12 cells in which tyrosine kinases such as Src and PYK 2 are highly abundant , stimulation by membrane depolarization in presence of extracellular calcium induces rapid and selective tyrosine phosphorylation of internalized light chain , the metalloprotease domain , of botulinum toxin A . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
A role for Pyk 2 and Src in linking G protein coupled receptors with MAP kinase activation . ^^^ Here we show that lysophosphatidic acid ( LPA ) and bradykinin induce tyrosine phosphorylation of Pyk 2 and complex formation between Pyk 2 and activated Src . ^^^ Moreover , tyrosine phosphorylation of Pyk 2 leads to binding of the SH 2 domain of Src to tyrosine 402 of Pyk 2 and activation of Src . ^^^ We propose that Pyk 2 acts with Src to link Gi and Gq coupled receptors with Grb 2 and Sos to activate the MAP kinase signalling pathway in PC 12 cells . . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
Collectively , these findings indicate that activation of FAK and Src is dependent on actin cytoskeleton integrity , Rho activation , and adhesion to extracellular matrix , whereas ERK activation is independent of these intracellular events and seems to correlate with activation of the newly identified protein tyrosine kinase PYK 2 . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
Furthermore , the apoptosis mediated by PYK 2 could be suppressed by over expressing catalytically active 5 Src , c Src , phosphatidylinositol 3 kinase , or Akt / protein kinase B . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
Although Src family kinases are activated by FcepsilonRI stimulation and tyrosine phosphorylate the receptor subunits , the activation and tyrosine phosphorylation of Pyk 2 were downstream of Syk . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
Fluoroaluminate induces activation and association of Src and Pyk 2 tyrosine kinases in osteoblastic MC3T3 E 1 cells . ^^^ Fluoroaluminate also induced activation of Src and the association of Pyk 2 with Src . ^^^ The phosphorylation of Src associated Pyk 2 increased > 20 fold in in vitro kinase assays , suggesting that Pyk 2 is phosphorylated by Src . ^^^ Although MC3T3 E 1 cells express much more Fak than Pyk 2 , Src preferentially associated with Pyk 2 . ^^^ In vitro , Pyk 2 bound to the Src SH 2 domain , suggesting that this interaction mediates the Src Pyk 2 association in cells . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
Furthermore , we identify two tyrosine residues that are subject to phosphorylation in response to muscarinic signaling and show that this phosphorylation induces two cytosolic proteins , c Src and Grb 2 , to bind to PYK 2 . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
Through reconstitution experiments in COS 1 cells , we determined that mouse PECAM 1 could be tyrosine phosphorylated by Src related PTKs and Csk related PTKs , but not by other kinases such as Syk , Itk , and Pyk 2 . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
PYK 2 in osteoclasts is an adhesion kinase , localized in the sealing zone , activated by ligation of alpha ( 5 ) beta 3 integrin , and phosphorylated by src kinase . ^^^ Taken together , these findings suggest that Src dependent tyrosine phosphorylation of PYK 2 is involved in the adhesion induced formation of the sealing zone , required for osteoclastic bone resorption . . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
Overexpression of both wild type ( WT ) and kinase inactive ( Ala 457 ) , but not the autophosphorylation site mutant ( Phe 402 ) Pyk 2 , enhanced endogenous FN stimulated c Src in vitro kinase activity in FAK cells , but only WT Pyk 2 overexpression enhanced FN stimulated activation of co transfected ERK 2 . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
It is a multi domain adapter protein capable of interacting with several structural and signaling proteins including vinculin , FAK , PYK 2 , Src and Crk . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
PMA treatment of FLG 29 . 1 cells induced a strong increase in the expression of p 120 Cbl and Pyk 2 kinase , which are putative Src substrates . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
In this report , we provide evidence that the protein tyrosine kinase Src and adaptor proteins Grb 2 , Crk , and p130Cas act as downstream mediators of Pyk 2 leading to the activation of extracellular signal regulated kinase ( ERK ) and c Jun amino terminal kinase ( JNK ) . ^^^ Pyk 2 induced activation of Src is necessary for phosphorylation of Shc and p130Cas and their association with Grb 2 and Crk , respectively , and for the activation of ERK and JNK cascades . ^^^ Expression of a Grb 2 mutant with a deletion of the amino terminal Src homology 3 domain or the carboxyl terminal tail of Sos strongly reduced Pyk 2 induced ERK activation , with no apparent effect on JNK activity . ^^^ Grb 2 with a deleted carboxyl terminal Src homology 3 domain partially blocked Pyk 2 induced ERK and JNK pathways , whereas expression of dominant interfering mutants of p130Cas or Crk specifically inhibited JNK but not ERK activation by Pyk 2 . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
Shrinkage stimulated tyrosine kinases Hck , Syk , and Pyk 2 , but prevention of their activation by the Src family inhibitor PP 1 failed to affect the L selectin response . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
Here , we have identified pp 55 and pp 130 as the adaptor molecules SKAP55hom / R ( Src kinase associated protein of 55 kDa homologue ) and FYB / SLAP 130 ( Fyn binding protein / SLP 76 associated protein of 130 kDa ) , respectively , and the tyrosine kinase activity as PYK 2 . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
We found that the Ang 2 induced tyrosine phosphorylation of Pyk 2 , which requires the activity of Src family kinase , was specifically regulated by the Src family kinase member , Yes kinase . ^^^ Moreover , we identified for the first time the constitutive association of Pyk 2 with an Src homology 2 ( SH 2 ) domain containing tyrosine phosphatase SHP 2 . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
N Acetylcysteine did inhibit the phosphorylation of the calcium sensitive tyrosine kinases PYK 2 and Src , effects that also occurred using nitric oxide . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
FIP 200 also inhibited the kinase activity of the Pyk 2 isolated from SYF cells ( deficient in Src , Yes , and Fyn expression ) and the Pyk 2 mutant lacking binding site for Src , suggesting that it regulated Pyk 2 kinase directly rather than affecting the associated Src family kinases . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
Carbachol stimulated transactivation of epidermal growth factor receptor and mitogen activated protein kinase in T ( 84 ) cells is mediated by intracellular ca ( 2+ ) , PYK 2 , and p 60 ( src ) . ^^^ CCh also induced tyrosine phosphorylation of p 60 ( src ) and association of p 60 ( src ) with both PYK 2 and the EGFR . ^^^ We conclude that CCh stimulated transactivation of the EGFR is mediated by a pathway involving elevations in intracellular Ca ( 2+ ) , calmodulin , PYK 2 , and p 60 ( src ) . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
ConA inhibited AT ( 1 ) receptor internalization without affecting ligand binding to the receptor , Ang 2 induced generation of second messengers , and activation of tyrosine kinases Src and Pyk 2 in vascular smooth muscle cells ( VSMC ) . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
HFS enhanced association of the src family kinases fyn and c src with an approximately 120 kDa tyrosine phosphorylated component containing the focal adhesion kinase ( FAK ) and its homologue PYK 2 . ^^^ Association of fyn with FAK and of c src with PYK 2 was increased following the HFS . ^^^ These results suggest that fyn and c src are involved in distinct signaling pathways and provide evidence for activation of FAK and PYK 2 following synaptic stimulation inducing LTP in vitro . . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
Furthermore , several signaling and adaptor molecules were found to be involved in alphavbeta 3 integrin dependent signaling pathways , including phosphatidylinositol 3 kinase , c Src , PYK 2 and p 130 ( cas ) . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
Interestingly , both Pyk 2 and FPhy 2 ( to a greater extent ) were tyrosine phosphorylated and associated with Src and Fyn . ^^^ This model is further supported by an inhibition of endogenous FAK association with active Src by Pyk 2 and FPhy 2 and a partial rescue by FAK of Pyk 2 mediated cell cycle inhibition . . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
We have previously reported that engagement of ( alpha ) ( 5 ) ( beta ) ( 3 ) integrin in osteoclasts induces tyrosine phosphorylation and activation of the adhesion kinase PYK 2 and the adaptor protein p 130 ( Cas ) in a Src dependent manner . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
Cbl associates with Pyk 2 and Src to regulate Src kinase activity , alpha ( 5 ) beta ( 3 ) integrin mediated signaling , cell adhesion , and osteoclast motility . ^^^ Using osteoclasts and transfected 293 cells , we find that a molecular complex comprising Src , Pyk 2 , and Cbl functions to regulate cell adhesion and motility . ^^^ The activation of integrin alpha ( 5 ) beta ( 3 ) induces the [ Ca ( 2+ ) ] ( 1 ) dependent phosphorylation of Pyk 2 Y402 , its association with Src SH 2 , Src activation , and the Src SH 3 dependent recruitment and phosphorylation of c Cbl . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
Here we report identification of PSGAP , a novel protein that interacts with PYK 2 and FAK and contains multiple domains including a pleckstrin homology domain , a rhoGTPase activating protein domain , and a Src homology 3 domain . ^^^ PYK 2 interacts with PSGAP Src homology 3 domain via the carboxyl terminal proline rich sequence . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
These data demonstrate that certain members of the Src family of kinases interact preferentially with the different isoforms of Pyk 2 and may have a role in the regulation of the Pyk 2 protein in lymphocytes . . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
Src deficient cells attach to but do not spread on vitronectin ( Vn ) coated surfaces and , contrary to wild type cells , their adhesion does not lead to tyrosine phosphorylation of molecules activated by adhesion , including PYK 2 , p 130 ( Cas ) , paxillin , and PLC gamma . ^^^ Furthermore , in Src ( / ) pOCs M CSF , together with filamentous actin , causes recruitment of beta ( 3 ) integrin and PLC gamma to adhesion contacts and induces stable association of beta ( 3 ) integrin with PLC gamma , phosphatidylinositol 3 kinase , and PYK 2 . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
Src and Pyk 2 mediate G protein coupled receptor activation of epidermal growth factor receptor ( EGFR ) but are not required for coupling to the mitogen activated protein ( MAP ) kinase signaling cascade . ^^^ The epidermal growth factor receptor ( EGFR ) and the non receptor protein tyrosine kinases Src and Pyk 2 have been implicated in linking a variety of G protein coupled receptors ( GPCR ) to the mitogen activated protein ( MAP ) kinase signaling cascade . ^^^ In this report we apply a genetic strategy using cells isolated from Src , Pyk 2 , or EGFR deficient mice to explore the roles played by these protein tyrosine kinases in GPCR induced activation of EGFR , Pyk 2 , and MAP kinase . ^^^ We show that Src kinases are critical for activation of Pyk 2 in response to GPCR stimulation and that Pyk 2 and Src are essential for GPCR induced tyrosine phosphorylation of EGFR . ^^^ By contrast , Pyk 2 , Src , and EGFR are dispensable for GPCR induced activation of MAP kinase . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
Inhibition of Src family tyrosine kinase activation with [ 4 Amino 5 ( 4 methylphenyl ) 7 ( t butyl ) pyrazolo [ 3 , 4 d ] pyrimidine ] or chelation of intracellular free calcium with 1 , 2 bis ( 2 aminophenoxy ) ethane N , N , N ' , N ' tetraacetic acid acetoxymethyl ester significantly decreased ET 1dependent p 21 ( ras ) activation and suggested the involvement of the cytoplasmic proline rich tyrosine kinase Pyk 2 . ^^^ Surprisingly , Src kinase activity was required upstream of ET 1 induced autophosphorylation of Pyk 2 . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
Although antioxidants had no effects on the Ca ( 2+ ) mobilization or phosphorylation of Ca ( 2+ ) dependent tyrosine kinase Pyk 2 , they inhibited c Src activation by Ang 2 , suggesting that c Src is 1 signaling molecule that links ROS and EGF R phosphorylation . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
Since the proline rich tyrosine kinase 2 ( Pyk 2 ) and Src were reported to be involved in the activation of the MAPKs in neuronal cells , we examined tyrosine phosphorylation and activation of Pyk 2 in the rat hippocampus after ECS . ^^^ At 1 min after ECS , the binding of Pyk 2 to Src and Grb 2 , and of Grb 2 to Ras increased . ^^^ These results suggested that ECS activates Pyk 2 , which then transmits the signal to MAPK cascade via Src , Grb 2 , and Ras in the rat hippocampus . . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
CD 44 initiated cell spreading induces Pyk 2 phosphorylation , is mediated by Src family kinases , and is negatively regulated by CD 45 . ^^^ Therefore , in CD 45 ( ) T cells , CD 44 signaling is mediated by Src family kinases , and this leads to Pyk 2 phosphorylation , cytoskeletal changes , and cell spreading . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
NMDA receptor activation results in tyrosine phosphorylation of NMDA receptor subunit 2A ( NR2A ) and interaction of Pyk 2 and Src with NR2A after transient cerebral ischemia and reperfusion . ^^^ In this study , effect of ischemia and reperfusion ( I / R ) on tyrosine phosphorylation of NMDA receptor subunit 2A ( NR2A ) and the interaction of two tyrosine kinases , Src and Pyk 2 , with NR2A was investigated . ^^^ Src and Pyk 2 co immunoprecipitated with NR2A and the binding increased after I / R , which also reached a peak at 6 h of reperfusion . ^^^ Besides , Src and Pyk 2 were activated after I / R . ^^^ Moreover , Src and Pyk 2 coprecipitated with each other . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
FAK regulates tyrosine phosphorylation of CAS , paxillin , and PYK 2 in cells expressing 5 Src , but is not a critical determinant of 5 Src transformation . ^^^ FAK was also shown to negatively regulate 5 Src mediated phosphorylation of the FAK related kinase PYK 2 . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
Paxillin associates with numerous signaling molecules including adaptor molecules ( p130Cas , CRK ) , kinases ( FAK , Pyk 2 , PAK and SRC ) , tyrosine phosphatases ( PTP PEST ) , ARF GAP proteins ( p95pkl , PAG 3 ) and papillomavirus E 6 oncoproteins . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
Ang 2 increased the association of Pyk 2 with Src and with the epidermal growth factor receptor ( EGF R ) . ^^^ Ang 2 induced activation of ERK , but not Pyk 2 , was prevented by inhibition of EGF receptor phosphorylation by AG 1478 and of Src kinase by PP 1 . ^^^ These findings indicate that Src and Pyk 2 act upstream of the EGF R and that the majority of Ang 2 induced ERK phosphorylation is dependent on trans activation of the EGF R . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
By using a compound that selectively interferes with EGFR ( AG 1478 ) , c Src ( PP 1 ) , or disrupts actin cytoskeleton ( cytochalasin D ) , we demonstrated that cytochalasin D completely inhibited the activation of Pyk 2 , but not that of EGFR , whereas AG 1478 did not inhibit the activation of Pyk 2 , indicating that transactivation of EGFR and signaling pathways involving Pyk 2 were distinct pathways . ^^^ EGFR was Ca ( 2+ ) independently activated and predominantly contributed to Shc / ERK / c fos activation , while Pyk 2 or c Src contributed less to it . . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
In the present study , we show that the Ca ( 2+ ) dependent pathway leading to activation of ERK1 / 2 is preceded by nonreceptor proline rich tyrosine kinase ( PYK 2 ) activation and epidermal growth factor ( EGF ) receptor tyrosine phosphorylation and is attenuated by inhibitors of src family kinases or the EGF receptor tyrosine kinase . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
We have also shown that acid stimulated NHE 3 activity depends on activation of Pyk 2 , c Src , MAP kinase , and the immediate early genes c Fos and c Jun . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
Thrombin stimulated Pyk 2 phosphorylation in human endothelium is dependent on intracellular calcium and independent of protein kinase C and Src kinases . ^^^ We have investigated the regulation of Pyk 2 in human umbilical vein endothelial cells in response to GPCAs and show that ( 1 ) thrombin , a PAR 1 peptide , and histamine cause rapid concentration and time dependent phosphorylation on tyrosines 402 ( Src kinase binding site ) , 881 ( Grb 2 binding site ) , and 580 ( an autophosphorylation site ) , ( 2 ) thrombin stimulated phosphorylation is dependent on intracellular calcium and independent of PKC and PI 3 kinase , and ( 3 ) inhibition of Src kinases has no significant effect on thrombin stimulated phosphorylation , implying that tyrosine phosphorylation of Pyk 2 is independent of Src binding . . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
Activation of Pyk 2 and Src / Fyn kinases led to increased tyrosine phosphorylation of NMDA receptor subunits 2A and B ( NR2A / B ) and was blocked by a selective mGluR 1 antagonist , 7 ( hydroxyamino ) cyclopropa [ b ] chromen 1a carboxylate ethyl ester , but not an mGluR 5 antagonist , 2 methyl 6 ( phenylethynyl ) pyridine . ^^^ Functional linkage between mGluR 1 activation and NR2A tyrosine phosphorylation through Pyk 2 and Src was also demonstrated after expression of these elements in human embryonic kidney 293 cells . ^^^ Furthermore , antagonizing calmodulin or mGluR 1 , but not PKC , reduced the basal tyrosine phosphorylation levels of Pyk 2 and Src , suggesting that mGluR 1 may control the basal activity of these kinases and thus the tyrosine phosphorylation levels of NMDA receptors . . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
We extend this hypothesis to downstream signalling molecules activated by ligation of the alphaVbeta 3 integrin , some of which ( i . e . , Src , PYK 2 , and Shc ) could have similar specific roles in tumor cells , activated endothelium and osteoclasts , but not in other cell types . . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
We have shown previously that the muscarinic agonist , carbachol ( CCh ) , transactivates the epidermal growth factor receptor ( EGFr ) via calmodulin , Pyk 2 , and Src kinase activation . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
A central role for Pyk 2 Src interaction in coupling diverse stimuli to increased epithelial NBC activity . ^^^ Pyk 2 phosphorylation was calcium / calmodulin dependent , and Pyk 2 associated with Src by means of SH 2 domain interaction . ^^^ Pyk 2 phosphorylation and Pyk 2 Src interaction by carbachol were mimicked by both ANG 2 and CO ( 2 ) . ^^^ To correlate Pyk 2 autophosphorylation and Pyk 2 Src interaction with NBC activity , cotransporter activity was measured in untransfected cells and in cells overexpressing Pyk 2 KD in the presence or absence of carbachol , ANG 2 , or CO ( 2 ) . ^^^ This coupling is mediated by Pyk 2 autophosphorylation and Pyk 2 Src interaction . . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
In addition , Ang 2 activates many intracellular tyrosine kinases that play a role in growth signaling and inflammation , such as Src , Pyk 2 , p130Cas , FAK and JAK / STAT . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
In the present study , we found that bovine ECs subjected to cyclic strain rapidly induced phosphorylation of PYK 2 and Src kinase . ^^^ This strain induced PYK 2 and Src phosphorylation was inhibited by pretreating ECs with an antioxidant N acetylcysteine . ^^^ Similarly , ECs exposed to H ( 2 ) O ( 2 ) increased both PYK 2 and Src phosphorylation . ^^^ An increased association of Src to PYK 2 was observed in ECs after cyclic strain or H ( 2 ) O ( 2 ) exposure . ^^^ ECs treated with an inhibitor to Src ( PPI ) greatly reduced Src and PYK 2 phosphorylation , indicating that Src mediated PYK 2 activation . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
Phenylephrine stimulated tyrosine phosphorylation of the EGFR , whereas carbachol stimulated p 60 ( Src ) , and possibly Pyk 2 , to activate MAPK . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
Although flow induced Cas phosphorylation was inhibited by kinase inactive Src , PYK 2 activation induced by flow was not inhibited by overexpression of kinase inactive Src . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
GnRH stimulation caused translocation of PKC alpha and epsilon to the cell membrane and enhanced the association of Src with PKC alpha and PKC epsilon , Pyk 2 , and the EGF receptor . ^^^ The Src inhibitor , PP 2 , the C terminal Src kinase ( Csk ) , and dominant negative Pyk 2 attenuated ERK1 / 2 activation by GnRH and PMA but not by EGF . ^^^ These findings indicate that Src and Pyk 2 act upstream of the EGF receptor to mediate its transactivation , which is essential for GnRH induced ERK1 / 2 phosphorylation in hypothalamic GnRH neurons . . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
PYK 2 autophosphorylation , but not kinase activity , is necessary for adhesion induced association with c Src , osteoclast spreading , and bone resorption . ^^^ Previous studies have shown that ligation of alpha ( 5 ) beta ( 3 ) integrin in osteoclasts induces c Src dependent tyrosine phosphorylation and PYK 2 activation , leading to cytoskeletal rearrangement , migration , and polarization of these cells . ^^^ By infecting pre fusion osteoclasts using recombinant adenovirus expressing PYK 2 and its mutants , we demonstrated that mutation at the autophosphorylation site ( Y402F ) abolishes PYK 2 association with c Src and reduces significantly phosphorylation at tyrosines 579 / 580 and 881 resulting in inhibition of osteoclast spreading and bone resorption . ^^^ Overexpression of the kinase dead PYK 2 ( K475A ) mutant had no effect on cell spreading , interaction with c Src , or the phosphorylation level of Tyr 402 , Tyr 579 / 580 , and Tyr 881 relative to PYK 2 ( wt ) expressing cells . ^^^ Whereas PYK 2 ( Y402F ) dominant negatively blocks osteoclast spreading and bone resorption , PYK 2 ( K457A ) may function in part as an adaptor by initially recruiting c Src to the adhesion complex , which appears to activate PYK 2 by phosphorylating additional tyrosines in its regulatory and C terminal domains . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
Other results suggest that rP2X ( 7 ) R mediated ERK1 / 2 phosphorylation was linked to the phosphorylation of the proline rich / Ca ( 2+ ) activated tyrosine kinase Pyk 2 , c Src , phosphatidylinositol 3 ' kinase , and protein kinase Cdelta activities and was dependent on the presence of extracellular Ca ( 2+ ) . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
Although p 190 RhoGAP is tyrosine phosphorylated by c Src via the tyrosine kinase Pyk 2 , wild type PTP 20 had little effect on p Tyr 418 of c Src and no effect on p Tyr 402 of Pyk 2 , which are required for full c Src activity and for interacting between Pyk 2 and c Src , respectively . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
Interaction of the tyrosine kinase Pyk 2 with the N methyl D aspartate receptor complex via the Src homology 3 domains of PSD 95 and SAP 102 . ^^^ We show that Pyk 2 effectively binds to the Src homology 3 ( SH 3 ) domain of SAP 102 only when the GK domain is removed from the SH 3 domain . ^^^ Characterization of PSD 95 and SAP 102 as adaptor proteins for Pyk 2 fills a critical gap in the understanding of the spatial organization of the Pyk 2 Src signaling pathway at the postsynaptic site and reveals a physiological function of the intramolecular SH 3 GK domain interaction in SAP102 . . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
Additionally , muscarinic receptors in PFC slices activated PKC and the focal adhesion kinase Pyk 2 ( a potential molecular link between PKC and Src ) in a PI3K dependent manner . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
The role of the Ca2+ sensitive tyrosine kinase Pyk 2 and Src in thrombin signalling in rat astrocytes . ^^^ Moreover , in response to thrombin stimulation Pyk 2 formed a complex with Src tyrosine kinase and adapter protein growth factor receptor bound protein 2 ( Grb 2 ) , which could be coprecipitated . ^^^ Furthermore , both thrombin induced Pyk 2 phosphorylation and extracellular signal regulated kinase ( ERK ) 1 / 2 phosphorylation can be attenuated by Src kinase inhibitor 4 amino 5 ( 4 chlorophenyl ) 7 ( t butyl ) pyrazolo [ 3 , 4 d ] pyrimidine . ^^^ From these data we conclude that PAR 1 uses Ca2+ and PKC dependent Pyk 2 to activate Src , thereby leading to ERK1 / 2 activation , which predominantly recruits Grb 2 in rat astrocytes . . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
PP 2 , an inhibitor of Src family tyrosine kinases , prevented the TLR induced phosphorylation of paxillin and Pyk 2 without affecting TLR induced IRAK activation . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
The experiments with KI mutants for various tyrosine kinases revealed that c Src and Pyk 2 ( but not JAK 1 , Jak 2 , Syk , and Tec ) are involved in ET 1 induced upregulation of Bcl xL expression . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
This adhesion induces recruitment and activation of the focal adhesion kinase Pyk 2 , the cytoskeleton linker paxillin , and the Src kinases Lck and Fyn in the contact site . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
Tyrosine phosphorylation of NR2A and interaction of NR2A with Src and Pyk 2 in hippocampus induced by brain ischemia and reperfusion ( I / R ) were determined by immunoprecipitation and immunoblot ( ting ) . ^^^ Interaction of NR2A with Src and Pyk 2 , tyrosine phosphorylation and kinase activity of Src and Pyk 2 also increased after I / R . ^^^ Src and Pyk 2 interacting with NR2A might also be involved in this regulation of the tyrosine phosphorylation of NR2A induced by I / R . . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
Here , using specific antibodies and inhibitors , we investigated the thrombin induced phosphorylation of Src family kinases , nonreceptor proline rich tyrosine kinase ( Pyk 2 ) , EGFR , and ERK1 / 2 . ^^^ Our results show that Src and Pyk 2 are involved upstream of the EGFR transactivation that is required for ERK1 / 2 phosphorylation . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
Interestingly , although the FAK related kinase Pyk 2 interacts with c Src and c Cbl , its activation is not disrupted in the absence of functional beta 3 . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
METHODS : Triton 10 insoluble ( actin cytoskeleton ) fractions from suspension phase cells and cells adherent to the beta ( 1 ) integrin ligand fibronectin were assessed for the presence of the integrin associated kinase Pyk 2 , the scaffolding protein paxillin , and the downstream Src family kinase Lyn . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
Following ischemia , tyrosine phosphorylation of NR2A and NR2B and activated Src family kinases ( SFKs ) and Pyk 2 were increased in post synaptic densities ( PSDs ) . ^^^ Ischemia induced increases in activated Pyk 2 and SFKs in PSDs , but not the translocation of PKC , Pyk 2 or Src to the PSD , were also inhibited by GF . ^^^ The results are consistent with a role for PKC in the ischemia induced increase in tyrosine phosphorylation of the NMDAR , via a pathway involving Pyk 2 and Src family kinases . . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
In contrast , both basal and ET induced PYK 2 phosphorylation were blocked by treatment with the Src family protein kinase inhibitor PP 2 . ^^^ These data demonstrate that ET induced PYK 2 activation is Gq , PKC epsilon , and Src dependent , describing a distinct signaling pathway leading to agonist induced PYK 2 activation in cardiomyocytes . . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
During development of the skeleton , osteoclast ( OC ) recruitment and migration are required for the vascular invasion of the cartilaginous anlage and the ossification of long bones . c Cbl lies downstream of the vitronectin receptor and forms a complex with c Src and Pyk 2 in a signaling pathway that is required for normal osteoclast motility . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
Stimulation of bone resorption by RANKL and , surprisingly , Src activity and Pyk 2 phosphorylation are normal in PTPepsilon deficient osteoclasts , indicating that loss of PTPepsilon does not cause widespread disruption of these signaling pathways . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
In addition , PSD 95 binds various regulatory proteins including Src , Pyk 2 , SynGAP , and nNOS and may recruit signaling proteins to NMDA receptors . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
However , neither the selective Src kinase inhibitor PP 2 nor the overexpression of negative regulatory Src kinase and dominant negative Pyk 2 had any effect on HB EGF induced responses . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
Pyk 2 and Src dependent tyrosine phosphorylation of PDK 1 regulates focal adhesions . 3 Phosphoinositide dependent protein kinase 1 ( PDK 1 ) is a signal integrator that activates the AGC superfamily of serine / threonine kinases . ^^^ The calcium activated tyrosine kinase Pyk 2 acts as a scaffold for Src dependent phosphorylation of PDK 1 on Tyr 9 , which permits phosphorylation of Tyr 373 and 376 by Src . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
Furthermore , the same cells plated onto osteopontin , adhere poorly and fail to phosphorylate c Src or Pyk 2 , two kinases central to organization of the osteoclast cytoskeleton . . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
Src forms a complex with Pyk 2 and Cbl after adhesion induced stimulation of alpha ( 5 ) beta ( 3 ) integrin . ^^^ To demonstrate the importance of the Pyk 2 Src association in osteoclasts and to distinguish the contributions of the Src adaptor and kinase activities in cytoskeletal organization and osteoclast function , we expressed mutants of Src and Pyk 2 in osteoclasts using adenovirus vectors . ^^^ Eliminating the Src binding site on Pyk 2 ( Pyk 2 ( Y402F ) ) markedly inhibited bone resorption by osteoclast like cells , whereas kinase dead Pyk 2 had little effect . ^^^ Kinase dead Src , unlike kinase dead Pyk 2 , markedly inhibited the bone resorbing activity of wild type osteoclasts and failed to significantly restore bone resorbing activity to Src ( / ) osteoclast like cells . ^^^ Activation of Src kinase by overexpressing kinase dead Csk failed to reverse the inhibitory effect of Pyk 2 ( Y402F ) , suggesting that osteoclastic bone resorption requires both c Src kinase activity and the targeting of Src kinase by Pyk 2 . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
A number of signalling molecules were found to be involved in the alpha ( 5 ) beta 3 integrin dependent signalling pathway , including c Src , Pyk 2 and p130Cas . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
These effects were completely dependent upon Tyr ( 402 ) , the autophosphorylation site of Pyk 2 , which allows recruitment of Src family members for further activating phosphorylations at other sites on Pyk 2 . ^^^ In the presence of activated FGFR 3 , the activation of Pyk 2 itself became independent of Tyr ( 402 ) , indicating that FGFR 3 activation circumvents the requirement for c Src recruitment at Tyr ( 402 ) of Pyk 2 . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
The association of Src family kinases with FAK and PYK 2 was also distinctively affected by ECS ; Src was mainly associated with PYK 2 while Yes was associated with FAK . ^^^ The phosphorylation of FAK and PYK 2 at the key tyrosine residue was not well correlated with the association with Src family kinases . . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
In this work , we investigated 17beta estradiol dependent signaling in platelets from adult healthy men . 17beta estradiol caused the rapid phosphorylation of the tyrosine kinases Src and Pyk 2 and the formation of a signaling complex , which included Src , Pyk 2 , and the phosphatidylinositol 3 kinase . ^^^ On treatment with 17beta estradiol , Src and Pyk 2 activation occurred in the membrane fraction but not in the cytosol . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
The Src family kinase Yes and the tyrosine phosphatase SHP 2 were detected in immunoprecipitates using either anti PYK 2 or anti EGFR antibodies . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
In this study , we examined the time course and the effect of lithium on Tyr 402 phosphorylation of Pyk 2 and Tyr 416 phosphorylation of Src as well as the association of Pyk 2 and NMDA receptor subunit 2A ( NR2A ) mediated by postsynaptic density protein 95 kDa ( PSD 95 ) in the condition of cerebral ischemia , which was induced by occlusion of the four vessels in Sprague Dawley rats . ^^^ At 6 h of reperfusion following 15 min of ischemia ( I / R ) , the effects induced by chronic lithium were observed , including the decrease in enhanced Tyr 402 phosphorylation of Pyk 2 , the inhibition in increased Tyr 416 phosphorylation of Src and the attenuation in enhanced interactions of Pyk 2 and PSD 95 with NR2A . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
These results implicate Dok 1 , Nck , Crk L , and Src kinases especially Lck , Pyk 2 , Zap 70 , Vav , and Ras GAP in intracellular signaling by SDF 1alpha / CXCL12 , and they suggest that Dok 1 plays an important role in SDF 1alpha / CXCL12 induced chemotaxis in T cells . . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
In contrast , another PKC inhibitor , Go 6976 [ 12 ( 2 cyanoethyl ) 6 , 7 , 12 , 13 tetrahydro 13 methyl 5 oxo 5H indolo ( 2 , 3 a ) pyrrolo ( 3 , 4 c ) carbazole ] , enhanced basal and agonist stimulated phosphorylation of ERK1 / 2 , which was not caused by alteration in receptor binding and internalization , stimulation of inositol phosphate production , or activation of Pyk 2 and Src tyrosine kinases . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
Pyk 2 , rather than PI 3K / Akt or Erk , appears to be the key downstream effecter of Src in mediating the cell survival signals . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
Phosphorylation of Alix by Src caused it to translocate from the membrane and cytoskeleton to the cytoplasm and reduced its interaction with binding partners SETA / CIN85 , epidermal growth factor receptor , and Pyk 2 . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
Incubation of OKP cells in acid media caused a transient increase in Pyk 2 phosphorylation that peaked at 30 seconds and increased Pyk2 / c Src binding at 90 seconds . ^^^ In addition , pyk 2 ( K457A ) blocked acid activation of c Src kinase , which is also required for acid regulation of NHE 3 . ^^^ The present results demonstrate that Pyk 2 is directly activated by acidic pH and that Pyk 2 activation is required for acid activation of c Src kinase and NHE 3 . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
SE resulted in the activation of Src and Pyk 2 associated with the postsynaptic apparatus , suggesting a role for these enzymes in the SE induced increase in tyrosine phosphorylation . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
The PKC proline rich tyrosine kinase ( Pyk 2 ) Src family kinase pathway may also mediate insulin potentiation . ^^^ A dominant negative Pyk 2 mutant significantly reduced insulin potentiation when co expressed with NR2B containing receptors , suggesting that Pyk 2 and downstream Src family tyrosine kinases are involved , along with PKCs , in insulin potentiation of NR2B . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
I / R led to increases of tyrosine phosphorylation of NR2A and interaction of Pyk 2 and Src kinase with NR2A after 6 h of reperfusion . ^^^ The antisense also inhibited the increased auto phosphorylation of Pyk 2 and Src kinase , while the protein expression of NR2A or Src kinase had no obvious change under the above conditions . ^^^ The data suggested that Pyk 2 may be involved in facilitating NR2A tyrosine phosphorylation by Src kinase after I / R . . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
Transient forebrain ischemia effects interaction of Src , FAK , and PYK 2 with the NR2B subunit of N methyl D aspartate receptor in gerbil hippocampus . ^^^ Two different models of brain ischemia were used to examine the evoked changes in the tyrosine phosphorylation of NMDA receptor subunits 2A and 2B ( NR2A and NR2B ) , as well as their interactions with non receptor tyrosine kinases ( NRTKs : FAK , PYK 2 Src ) , and PSD 95 protein . ^^^ Concomitantly , an increased association of NR2B with FAK , PYK 2 , Src and PSD 95 has been observed . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
After integrin engagement , Pyk 2 recruits Src and the adaptor protein Cbl , forming a molecular signaling complex that is critical for cell migration , and deletion of any molecule in this complex disrupts podosome ring formation and / or decreases osteoclast migration . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
VEGF also stimulated phosphorylation of Pyk 2 ; VEGF induced phosphorylation of Pyk 2 , c Src and Raf 1 could be abolished by BAPTA / AM , demonstrating requirement for induction of intracellular calcium currents . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
In cultured bovine adrenal glomerulosa cells , LPA , which is predominantly coupled to Gi and partially to Gq / protein kinase C alpha and epsilon , caused phosphorylation of Src ( at Tyr 416 ) , proline rich tyrosine kinase ( Pyk 2 at Tyr 402 ) , EGF R , protein kinase B / Akt , extracellularly regulated signal kinases 1 / 2 , and their dependent protein , p 90 ribosomal S 6 kinase . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
Semaphorin 4D / plexin B 1 induces endothelial cell migration through the activation of PYK 2 , Src , and the phosphatidylinositol 3 kinase Akt pathway . ^^^ Surprisingly , we found that Plexin B 1 stimulates PI3K Akt through the activation of an intracellular tyrosine kinase cascade that involves the sequential activation of PYK 2 and Src . ^^^ This results in the tyrosine phosphorylation of Plexin B 1 , the rapid recruitment of a multimeric signaling complex that includes PYK 2 , Src , and PI3K to Plexin B 1 and the activation of Akt . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
Ang 2 induced AT1R activation via Gq / 11 stimulates phospholipases A 2 , C , and D , and activates inositol trisphosphate / Ca2+ signaling , protein kinase C isoforms , and MAPKs , as well as several tyrosine kinases ( Pyk 2 , Src , Tyk 2 , FAK ) , scaffold proteins ( G protein coupled receptor kinase interacting protein 1 , p130Cas , paxillin , vinculin ) , receptor tyrosine kinases , and the nuclear factor kappaB pathway . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
Herein , we show that FAK expression is essential for alpha5beta1 stimulated cell motility and that exogenous expression of human alpha 4 in FAK null fibroblasts forms a functional alpha4beta1 receptor that promotes robust cell motility equal to the alpha5beta1 stimulation of wild type and FAK reconstituted fibroblasts . alpha4beta1 stimulated FAK null cell spreading and motility were dependent on the integrity of the alpha 4 cytoplasmic domain , independent of direct paxillin binding to alpha 4 , and were not affected by PRNK expression , a dominant negative inhibitor of Pyk 2 . alpha 4 cytoplasmic domain initiated signaling led to a approximately 4 fold activation of c Src which did not require paxillin binding to alpha 4 . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
In washed platelets , coincubation with neutrophils resulted in reduced formation of large aggregates when stimulated with collagen or thrombin and repressed agonist induced activation of tyrosine protein kinases ( Syk , Lyn , Src , and Pyk 2 ) , but not thrombin induced ERK and p 38 MAP kinase . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
Once recruited to the activated integrins , especially alphavbeta 3 ) , by the adhesion tyrosine kinase Pyk 2 , Src binds and phosphorylates Cbl and Cbl b , homologous multisite adapter proteins with ubiquitin ligase activity . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
Since RhoA activity is associated with actomyosin contractility , markers of this signaling cascade were assessed including phosphorylated myosin light chain ( MLC ) , cofilin , Pyk 2 , Src , and MAPK kinases . ^^^ Thus , the positive regulation of E cadherin adhesion provided by Apc+ in vivo allows proper negative regulation of Egfr , Src , Pyk 2 , and MAPK , as well as RhoA activities . . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
In vascular smooth muscle cells ( VSMCs ) , through its G protein coupled AngII Type 1 receptor ( AT ( 1 ) ) , AngII activates various intracellular protein kinases , such as receptor or non receptor tyrosine kinases , which includes epidermal growth factor receptor ( EGFR ) , platelet derived growth factor receptor ( PDGFR ) , c Src , PYK 2 , FAK , JAK 2 . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
Much of the cytoplasmic signal transduction activity associated with HBx expression and its stimulation of viral replication is attributable to HBx induced activation of calcium signaling pathways involving Pyk 2 and Src tyrosine kinases . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
We here highlight the contributions of the Src adaptor and kinase activities in cytoskeletal organization and osteoclast function using adenovirus vectors containing various mutants of Src or Pyk 2 . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
Therefore , tyrosine phosphorylation dependent signaling involving receptor tyrosine kinases , mitogen activated protein kinases , Abl , Src , and Pyk 2 is known to be initiated or amplified by reactive oxidants . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
However , when the most upstream signaling molecules in this pathway , non receptor protein tyrosine kinases ( PTKs ) such as Syk , ZAP 70 , Pyk 2 and Src were examined , no significant activation was seen . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
Roles of protein kinase C , Ca2+ , Pyk 2 , and c Src in agonist activation of rat lacrimal gland p42 / p44 MAPK . ^^^ Activated MAPK , Pyk 2 , and c Src amounts were measured by Western blot analysis . ^^^ Carbachol increased the phosphorylation of Pyk 2 on tyrosine 402 and c src on tyrosine 416 in a time dependent manner . ^^^ The c src inhibitor PP 1 inhibited carbachol stimulated phosphorylation of Pyk 2 . ^^^ CONCLUSIONS : It was concluded that cholinergic agonists use Ca ( 2+ ) and PKC to phosphorylate Pyk 2 and c Src , which subsequently stimulate MAPK activity . ^^^
Interacting proteins: Q14289 and P12931	Pubmed	SVM Score :0.0
Reduced NMDA receptor tyrosine phosphorylation in PTPalpha deficient mouse synaptosomes is accompanied by inhibition of four src family kinases and Pyk 2 : an upstream role for PTPalpha in NMDA receptor regulation . ^^^