| Interacting proteins: Q13480 and P12931 |
Pubmed |
SVM Score :1.1263979 |
| Moreover , Src interacted with Gab 1 via both its Src homology 2 and 3 domains and was capable of phosphorylating purified Gab 1 in vitro . 1.1263979^^^ |
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| Interacting proteins: Q13480 and P12931 |
Pubmed |
SVM Score :0.0 |
| We previously found that EGF ( epidermal growth factor ) increases the EGFR ( EGF receptor ) kinase binding affinity towards the major tyrosine phosphorylation sites in downstream adaptor proteins such as Gab 1 ( Grb 2 associated binding protein 1 ) and Shc [ Src homology 2 ( SH 2 ) domain and collagen containing protein ] , but not that towards EGFR autophosphorylation sites [ Fan , Wong , Deb and Johnson ( 2004 ) J . ^^^ |
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| Interacting proteins: Q13480 and P12931 |
Pubmed |
SVM Score :0.0 |
| We show that phosphoinositide 3 ( PI 3 ) kinase associates with 4 tyrosine phosphorylated proteins in primary human erythroid progenitors , namely insulin receptor substrate 2 ( IRS 2 ) , Src homology 2 domain containing inositol 5 ' phosphatase ( SHIP ) , Grb 2 associated binder 1 ( Gab 1 ) , and the Epo receptor ( EpoR ) . ^^^ |
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| Interacting proteins: Q13480 and P12931 |
Pubmed |
SVM Score :0.0 |
| Membrane targeting of Grb 2 associated binder 1 ( Gab 1 ) scaffolding protein through Src myristoylation sequence substitutes for Gab 1 pleckstrin homology domain and switches an epidermal growth factor response to an invasive morphogenic program . ^^^ |
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| Interacting proteins: Q13480 and P12931 |
Pubmed |
SVM Score :0.0 |
| Grb 2 associated binder 1 ( Gab 1 ) is a scaffolding / docking protein and contains a Pleckstrin homology domain and potential binding sites for Src homology ( SH ) 2 and SH 3 domains . ^^^ |
|
| Interacting proteins: Q13480 and P12931 |
Pubmed |
SVM Score :0.0 |
| Phosphorylated Gab 1 acts as a docking protein for Src homology 2 ( SH 2 ) domain containing proteins . ^^^ |
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| Interacting proteins: Q13480 and P12931 |
Pubmed |
SVM Score :0.0 |
| Our studies show that activated Met associates with , and phosphorylates , the docking protein Gab 1 , which in turn binds to the src homology 2 ( SH 2 ) domain of the adapter protein Crk and recruits Crk to the Met signaling complex . ^^^ |
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| Interacting proteins: Q13480 and P12931 |
Pubmed |
SVM Score :0.0 |
| Furthermore , pretreatment with the selective Src family kinase inhibitor PP 2 completely inhibited the ability of sorbitol treatment to cause tyrosine phosphorylation of Gab 1 . ^^^ |
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| Interacting proteins: Q13480 and P12931 |
Pubmed |
SVM Score :0.0 |
| The Src homology 2 ( SH 2 ) domain of the phosphatidylinositol 3 kinase ( PI3K ) regulatory subunit binds Gab 1 in a phosphorylation independent manner . ^^^ |
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| Interacting proteins: Q13480 and P12931 |
Pubmed |
SVM Score :0.0 |
| Src family tyrosine kinases , phosphoinositide 3 kinase and Gab 1 regulate extracellular signal regulated kinase 1 activation induced by the type A endothelin 1 G protein coupled receptor . ^^^ Tyrosine phosphorylation of Gab 1 induced by endothelin 1 was inhibited by PP 1 , a pharmacological inhibitor of Src family tyrosine kinases . ^^^ Structure function analyses of Gab 1 indicated that mutant forms of Gab 1 that do not bind the Src homology ( SH ) 2 domains of the p 85 adapter subunit of PI 3 kinase or the SH 2 domain containing protein tyrosine phosphatase 2 ( SHP 2 ) were impaired in their ability to potentiate ET 1 induced ERK 1 activation . ^^^ Taken together , our data indicate that PI 3 kinase and Src family tyrosine kinases regulate ET 1 induced Gab 1 tyrosine phosphorylation , which , in turn , induces ERK 1 activation via PI 3 kinase and SHP 2 dependent pathways . . ^^^ |
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| Interacting proteins: Q13480 and P12931 |
Pubmed |
SVM Score :0.0 |
| Inhibiting ERK activation with the MEK inhibitor U 0126 increased the EGF stimulated association of Gab 1 with either full length glutathione S transferase p 85 or the p 85 C terminal Src homology 2 ( SH 2 ) domain , a result reproduced by co immunoprecipitation of the native proteins from intact cells . ^^^ |
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| Interacting proteins: Q13480 and P12931 |
Pubmed |
SVM Score :0.0 |
| Upon stimulation of the bombesin receptors , KUZ increases the docking and activation of adaptors Src homology 2 domain containing protein and Gab 1 on the EGFR , and activation of Ras and Erk . ^^^ |
|
| Interacting proteins: Q13480 and P12931 |
Pubmed |
SVM Score :0.0 |
| We find in 3T3 F442A preadipocytes that GH promotes tyrosine phosphorylation of Gab 1 and its association with SHP 2 , an Src homology 2 containing cytoplasmic tyrosine phosphatase . ^^^ |
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| Interacting proteins: Q13480 and P12931 |
Pubmed |
SVM Score :0.0 |
| Development of an efficient `` substrate trapping ' ' mutant of Src homology phosphotyrosine phosphatase 2 and identification of the epidermal growth factor receptor , Gab 1 , and three other proteins as target substrates . ^^^ |
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| Interacting proteins: Q13480 and P12931 |
Pubmed |
SVM Score :0.0 |
| Gab 1 phosphorylation is sensitive to the Src family kinase inhibitor PP 2 . ^^^ |
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| Interacting proteins: Q13480 and P12931 |
Pubmed |
SVM Score :0.0 |
| Roles of Gab 1 and SHP 2 in paxillin tyrosine dephosphorylation and Src activation in response to epidermal growth factor . ^^^ Expression of an SHP 2 binding defective mutant of Gab 1 ( Gab1FF ) or a catalytically inactive mutant of SHP 2 ( SHP2DN ) prevented paxillin tyrosine dephosphorylation and Src activation induced by EGF . ^^^ Together , these results reveal that Gab 1 recruits SHP 2 to dephosphorylate paxillin , leading to dissociation of Csk from the paxillin Src complex and Src activation and that Src is an SHP 2 effector involved in EGF stimulated Erk activation and cell migration . . ^^^ |
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| Interacting proteins: Q13480 and P12931 |
Pubmed |
SVM Score :0.0 |
| Genetic and pharmaceutical analyses suggest the involvement of c Met and the Src family tyrosine kinases in mediating UV induced Gab 1 phosphorylation as well as JNK activation . ^^^ |
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| Interacting proteins: Q13480 and P12931 |
Pubmed |
SVM Score :0.0 |
| Tumors exhibited increased association of Egfr with clathrin heavy chain ( CHC ) , Gab 1 , and p85alpha , the regulatory subunit of phosphoinositide 3 kinase ( PI3K ) , and tumors also overexpressed c Src , PDK 1 , and Akt . ^^^ |
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| Interacting proteins: Q13480 and P12931 |
Pubmed |
SVM Score :0.0 |
| This leads to a G protein / c Src dependent tyrosine phosphorylation of Gab 1 and accumulation of dynamin 2 at the plasma membrane , a step required for endocytosis of the PDGFbeta receptor GPCR complex . ^^^ |
|
| Interacting proteins: Q13480 and P12931 |
Pubmed |
SVM Score :0.0 |
| Gab 1 phosphorylation as well as activation of Akt and eNOS by flow was inhibited by the Src kinase inhibitor PP 2 ( 4 amino 5 ( 4 chlorophenyl ) 7 ( t butyl ) pyrazolo [ 3 , 4 d ] pyrimidine ) and VEGFR 2 kinase inhibitors SU 1498 and VTI , suggesting that flow mediated Gab 1 phosphorylation is Src kinase dependent and VEGFR 2 dependent . ^^^ |
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| Interacting proteins: Q13480 and P12931 |
Pubmed |
SVM Score :0.0 |
| To conduce these activities , the cytoplasmic C terminal region of this receptor acts as a docking site for multiple protein substrates , including Grb 2 , Gab 1 , STAT 3 , Shc , SHIP 1 and Src . ^^^ |
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| Interacting proteins: Q13480 and P12931 |
Pubmed |
SVM Score :0.0 |
| By mutational analysis , we identified that Src homology 2 domain of Crk is indispensable for the induction of the phosphorylation on multiple Tyr 10 10 Pro motifs containing Y 307 in Gab 1 . ^^^ |
|
| Interacting proteins: Q13480 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13480 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13480 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13480 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13480 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13480 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13480 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13480 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13480 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13480 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13480 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13480 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13480 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13480 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13480 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13480 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13480 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13480 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|