| Interacting proteins: Q07954 and P12931 |
Pubmed |
SVM Score :0.0 |
| Reelin , a large protein that regulates neuronal migration during embryonic development , activates a conserved signaling pathway that requires its receptors , very low density lipoprotein receptor and apolipoprotein E receptor 2 , the cytoplasmic adaptor protein Disabled 1 ( Dab 1 ) , and Src family kinases ( SFK ) . ^^^ |
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| Interacting proteins: Q07954 and P12931 |
Pubmed |
SVM Score :0.0 |
| Our results show that platelet derived growth factor ( PDGF ) BB induces a transient tyrosine phosphorylation of the LRP cytoplasmic domain in a process dependent on PDGF receptor activation and c Src family kinase activity . ^^^ The use of LRP mini receptor mutants as well as in vitro phosphorylation studies demonstrated that the tyrosine located within the second NPXY motif found in the LRP cytoplasmic domain is the primary site of tyrosine phosphorylation by Src and Src family kinases . ^^^ |
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| Interacting proteins: Q07954 and P12931 |
Pubmed |
SVM Score :0.0 |
| Furthermore , the cytoplasmic domain of the LDL receptor related protein ( LRP ) was found to be a substrate for the non receptor tyrosine kinase Src , but the physiological significance of this phosphorylation event remained unknown . ^^^ Chem . 277 , 15499 15506 ) Loukinova et al . further demonstrate that one form of PDGF , PDGF BB , binds specifically to LRP and that phosphorylation of LRP requires the activation of Src family kinases . ^^^ |
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| Interacting proteins: Q07954 and P12931 |
Pubmed |
SVM Score :0.0 |
| Using a panel of cytoplasmic domain mutants of the chimeric M CSF / PDGFR beta , we confirmed that the kinase domain of PDGFR beta is absolutely required for LRP tyrosine phosphorylation but that PDGFR beta mediated activation of phosphatidylinositol 3 kinase , RasGAP , SHP 2 , phospholipase C gamma , and Src are not necessary for LRP tyrosine phosphorylation . ^^^ |
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| Interacting proteins: Q07954 and P12931 |
Pubmed |
SVM Score :0.0 |
| We recently observed that the LDL receptor related protein 1 ( LRP 1 ) is tyrosine phosphorylated in 5 Src transformed cells . ^^^ Using a GST fusion protein containing the cytoplasmic domain of LRP 1 , we show that LRP 1 is a direct substrate for 5 Src in vitro . ^^^ Of the four tyrosine residues present in the cytoplasmic domain of LRP 1 , only Tyr 63 is phosphorylated by 5 Src in vivo or in vitro . ^^^ Using fibroblasts deficient in Src , Yes and Fyn , we were able to show that there are multiple kinases present in the cell that can phosphorylate LRP 1 . ^^^ |
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| Interacting proteins: Q07954 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q07954 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q07954 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q07954 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q07954 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q07954 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q07954 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q07954 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q07954 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q07954 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q07954 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q07954 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q07954 and P12931 |
Pubmed |
SVM Score :0.0 |
| Tyrosine phosphorylated low density lipoprotein receptor related protein 1 ( Lrp 1 ) associates with the adaptor protein SHC in SRC transformed cells . 5 Src transforms fibroblasts in vitro and causes tumor formation in the animal by tyrosine phosphorylation of critical cellular substrates . ^^^ We purified a 100 kDa Shc PTB binding protein from Src transformed cells that was identified as the beta chain of the low density lipoprotein receptor related protein LRP 1 . ^^^ This study shows that LRP 1 is tyrosine phosphorylated in 5 Src transformed cells and that tyrosine phosphorylated LRP 1 binds in vivo and in vitro to Shc . ^^^ The association between Shc and LRP 1 may provide a mechanism for recruitment of Shc to the plasma membrane where it is phosphorylated by 5 Src . ^^^ |
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