Pubmed abstracts for Protein-Protein Interaction search result :

Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.63495391
Taken together with all these findings , it is suggested that c Src directly phosphorylates Shc on tyrosine residues , which in turn binds to Grb 2 constitutively associated with Sos to form a Shc Grb 2 Sos complex , and that the complex formation is coupled with MAP kinase activation mediated by Ras activation in U 937 cells . . 0.63495391^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.55102409
Pyk 2 induced activation of Src is necessary for phosphorylation of Shc and p130Cas and their association with Grb 2 and Crk , respectively , and for the activation of ERK and JNK cascades . 0.55102409^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.9648521
Here , we demonstrate that Grb 2 bound to tyrosine phosphorylated FRS 2 alpha forms a ternary complex with Cbl by means of its Src homology 3 domains resulting in the ubiquitination of fibroblast growth factor ( FGF ) receptor and FRS 2 alpha in response to FGF stimulation . 0.9648521^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
The shc oncogene product is tyrosine phosphorylated by Src family kinases and after its phosphorylation interacts with the adapter protein Grb 2 ( growth factor receptor bound protein 2 ) . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
After its stimulation , there was an increase in the association of the src cytoplasmic tyrosine kinase Fyn and the adapter protein Grb 2 . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
After binding to EGFR , PTP1B becomes tyrosine phosphorylated at Tyr 66 , a site that conforms to the consensus binding sequence for the Src homology 2 ( SH 2 ) domains of the adapter protein Grb 2 . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
Integrin engagement caused the formation of phosphorylated pp 60 ( src ) / focal adhesion kinase complexes and the aggregation of focal adhesion plaques containing the beta ( 3 ) integrin subunit , the cytoskeletal proteins vinculin , cortactin , and paxillin , the tyrosine kinases focal adhesion kinase and pp 60 ( src ) , the adapter protein Grb 2 , and the mitogen activated protein kinase ERK 2 . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
Signalling by the insulin receptor substrate ( IRS ) proteins is critically dependent on the tyrosine phosphorylation of specific binding sites that recruit Src homology 2 ( SH 2 ) domain containing proteins , such as the p 85 subunit of phosphoinositide 3 kinase ( PI 3 kinase ) , the tyrosine phosphatase SHP 2 and the adapter protein Grb 2 . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
Combined cell lysates were affinity purified over the SH 2 domain of the adapter protein Grb 2 ( GST SH 2 fusion protein ) that specifically binds phosphorylated EGFR and Src homologous and collagen ( Shc ) protein . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
A tyrosine phosphorylated peptide of the epidermal growth factor receptor specifically retrieved the Src homology domain ( SH ) 2 and SH 3 domain containing adapter protein Grb 2 . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
A cDNA clone encoding a novel , widely expressed protein ( called growth factor receptor bound protein 2 or GRB 2 ) containing one src homology 2 ( SH 2 ) domain and two SH 3 domains was isolated . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
Bacterially expressed growth factor receptor bound protein 2 ( GRB 2 ) and the SH 3 domain of c Src were also found to stimulate GTPase activity , although to a lesser extent than microtubules . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
These results suggest , for the first time , that TGF beta induces down regulation of Src family kinases , leading to disruption of the SHC growth factor receptor bound protein 2 complex . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
The association of the src homology 2 ( SH 2 ) domain containing tyrosine phosphatase ( SH PTP 2 ) with the activated epidermal growth factor ( EGF ) and platelet derived growth factor receptors , as well as the insulin receptor substrate 1 and growth factor receptor bound protein 2 and its intrinsic tyrosine phosphatase activity suggests an important role for this phosphatase in signal transduction . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
BACKGROUND : Growth factor receptor bound protein 2 ( GRB 2 ) is an adaptor protein with three Src homology ( SH ) domains in the order SH 3 SH2 SH 3 . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
A short , proline rich region spanning residues 566 577 in human 5 lipoxygenase is a binding site for the Src homology 3 ( SH 3 ) domain of growth factor receptor bound protein 2 ( Grb 2 ) , an `` adaptor ' ' protein for tyrosine kinase mediated cell signaling . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
Growth factor receptor bound protein 2 ( Grb 2 ) links tyrosine phosphorylated proteins to a guanine nucleotide releasing factor of the son of sevenless ( Sos ) class by attaching to the former by its Src homology 2 ( SH 2 ) moiety and to the latter by its SH 3 domains . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
Here , we show that insulin induced activation of ERKs was enhanced by stable overexpression of growth factor receptor bound protein 2 ( GRB 2 ) but not by overexpression of GRB 2 proteins with point mutations in the Src homology 2 and 3 domains . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
Proteins containing Src homology 2 and 3 ( SH 2 and SH 3 ) domains , such as the p 85 regulatory subunit of phosphatidylinositol 3 kinase and growth factor receptor bound protein 2 ( GRB 2 ) , bind tyrosine phosphate sites on IRS 1 through their SH 2 regions . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
Therefore , we investigated the effects of IL 6 on some recently identified signaling intermediates , Shc ( Src homology and collagen ) and Grb 2 ( growth factor receptor bound protein 2 ) , known to activate p21ras . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
Hematopoietic cell transformation by p2l0bcr / abl seems to involve activation of the Ras signaling pathway by at least two different signaling intermediates , growth factor receptor bound protein 2 and Src homology and collagen protein , but additional signaling proteins are likely to be required as well . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
Nuclear magnetic resonance solution structure of the growth factor receptor bound protein 2 Src homology 2 domain . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
In pp 125 ( FAK ) expressing cells , this was concomitant with increased association of IRS 1 with Src homology 2 containing proteins such as growth factor receptor bound protein 2 , phosphatidylinositol ( PI ) 3 kinase p85alpha subunit , and Src homology 2 containing protein tyrosine phosphatase 2 . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NS5A , a nonstructural protein of hepatitis C virus , binds growth factor receptor bound protein 2 adaptor protein in a Src homology 3 domain / ligand dependent manner and perturbs mitogenic signaling . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
EGF and NGF strongly activated tyrosine phosphorylation of Shc and caused association of Src homology collagen ( Shc ) with growth factor receptor bound protein 2 ( Grb 2 ) ; however , neither NE nor UTP caused substantial activation of the Shc / Grb2 pathway . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
The interaction of the activated epidermal growth factor ( EGF ) receptor ( EGFR ) with the Src homology 2 ( SH 2 ) domain of Grb 2 ( growth factor receptor bound protein 2 ) initiates signalling through Ras and mitogen activated protein kinase . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
Treatment with 17 AAG decreased NPM ALK expression and phosphorylation , thus impairing its association with phospholipase C gamma , Src homology 2 domain containing protein ( Shc ) , growth factor receptor bound protein 2 ( Grb 2 ) , and insulin receptor substrate 1 ( IRS 1 ) . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
Moreover , in response to thrombin stimulation Pyk 2 formed a complex with Src tyrosine kinase and adapter protein growth factor receptor bound protein 2 ( Grb 2 ) , which could be coprecipitated . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
Blocking the interaction between phosphotyrosine ( pTyr ) containing activated receptors and the Src homology 2 ( SH 2 ) domain of the growth factor receptor bound protein 2 ( Grb 2 ) is considered to be an effective and non cytotoxic strategy to develop new anti proliferative agents due to its potential to shut down the Ras activation pathway . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
Prolidase activity and collagen biosynthesis are supposed to be regulated by beta ( 1 ) integrins , which initiate a signaling pathway in which several kinases and intracellular proteins are involved , including focal adhesion kinase pp 125 ( FAK ) ( FAK ) , Src , Shc , growth factor receptor bound protein 2 ( Grb 2 ) , son of sevenless protein ( SOS ) , Ras , Raf and mitogen activated protein kinases ( MAPK ) , extracellular signal regulated kinase 1 ( ERK ( 1 ) ) and kinase 2 ( ERK ( 2 ) ) . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
The protein ASH ( for abundant Src homology ) , composed of one Src homology region ( SH ) 2 and two SH 3 domains , was cloned by screening human and rat cDNA libraries with an oligonucleotide probe directed to a consensus sequence of the SH 2 domains . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
The protein ASH ( for abundant Src homology ) ( Matuoka , K . , Shibata , M . , Yamakawa , A . , and Takenawa , T . ( 1992 ) Proc . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
Tyrosine phosphorylated IRS 1 binds the 85K subunit of phosphatidylinositol 3 kinase which may be involved in the translocation of glucose transporters and the abundant src homology protein ( ASH ) / Grb2 which may be involved in activation of p21ras and MAP kinase cascade . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
Association of Ash / Grb 2 with dynamin through the Src homology 3 domain . ^^^ Ash / Grb 2 is an adaptor protein composed only of Src homology ( SH ) 2 and SH 3 domains that is considered to be essential for Ras activation . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
Nck is a small protein composed of Src homology regions ( SH ) 2 and 3 , paralleling the adaptors c Crk and Grb2 / Ash , but its function remains enigmatic . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
The chemical speciation of Ni in fly ash produced from approximately 0 . 85 wt % S residual ( no . 6 fuel ) oils in laboratory ( 7 kW ) and utility ( 400 MW ) scale combustion systems was investigated using 10 ray absorption fine structure ( XAFS ) spectroscopy , 10 ray diffraction ( XRD ) , and acetate extraction [ 1 M NaOAc 0 . 5 M HOAc ( pH 5 ) at 25 degrees C ] anodic stripping voltammetry ( ASV ) . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
In this report we demonstrate that Grb 2 mutant proteins lacking amino or carboxyl terminal src homology SH 3 domains suppress BCR ABL induced Ras activation and reverse the oncogenic phenotype . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
Distinct peptide sequences which bind selectively with Src and Grb 2 SH2 domains were also prepared with pTyr and F2Pmp . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
Phosphotyrosine peptide binding to Grb 2 induces tryptophan fluorescence changes in the Src homology 2 ( SH 2 ) domain . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
The Src homology 2 ( SH 2 ) domain of the adaptor protein Grb 2 bound with high affinity to tyrosine phosphorylated SHPTP 2 following treatment of cells with IL 3 or GM CSF , but not IL 4 . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
Affinity chromatography for purification of the modular protein growth factor receptor bound protein 2 and development of a screening test for growth factor receptor bound protein 2 Src homology 3 domain inhibitor using peroxidase linked ligand . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
Nucleotide exchange stimulation by PE is prevented by PKC ( protein kinase C ) inhibition but not by EGFR [ EGF ( epidermal growth factor ) receptor ] blockade , despite the fact that EGFR inhibition aborts basal and PE induced Shc ( Src homology and collagen homology ) phosphorylation and Shc Grb 2 ( growth factor receptor bound protein 2 ) association . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
The latter finding , in conjunction with the observations that p 145 does not bind , in vitro , to the Src homology 2 ( SH 2 ) domain of Shc , that it is not present in anti Grb 2 immunoprecipitates , and that a phosphopeptide which blocks the binding of Shc to the SH 2 domain of Grb 2 also blocks the binding of Shc to p 145 , suggests that p 145 contains an SH 2 domain and competes with Grb 2 for the same tyrosine phosphorylated site on Shc . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
One pathway by which ERKs 1 and 2 are activated by tyrosine kinases involves the Src homology ( SH ) 2 containing proteins SHC and Grb 2 . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
Mitotic , tyrosine phosphorylated Sam 68 bound selectively to recombinant SH 2 domains with significantly different affinities ( c Src approximately Ras GTPase activating protein > p 85 alpha ( amino terminal ) > Grb 2 > > p 85 alpha ( COOH terminal ) ) . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
The resulting constrained pTyr mimetics were examined for inhibitory potency in six SH 2 domain constructs : Lck , Src , Grb 2 , and the C terminal SH 2 domains of PLC gamma ( PLC gamma C ) and the p 85 subunit of PI 3 kinase ( p 85 C ) , as well as the N terminal SH 2 domain of SH PTP 2 . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
Receptor tyrosine kinases couple to Ras through the Src homology 2 ( SH 2 ) domain protein Grb 2 . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
Single amino acid substitutions in the GRB 2 binding site ( Y177F ) , Src homology 2 domain ( R552L ) , or an autophosphorylation site in the tyrosine kinase domain ( Y793F ) do not diminish the antiapoptotic and transforming properties of BCR ABL in hematopoietic cells , although these mutations were previously shown to drastically reduce the transforming activity of BCR ABL in fibroblasts . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
PI 3 kinase and GRB 2 bind to the CD 28 phosphotyrosine based Tyr Met Asn Met motif by means of intrinsic Src homology 2 ( SH 2 ) domains . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
We demonstrate that the K protein contains a cluster of at least three SH 3 binding sites ( P 1 , PPGRGGRPMPPSRR , amino acids 265 278 ; P 2 , PRRGPPPPPPGRG , 285 297 ; and P 3 , RARNLPLPPPPPPRGG , 303 318 ) and that each one of these sites is capable of selectively engaging c Src and Vav SH 3 domains but not SH 3 domains of Abl , p 85 phosphatidylinositol 3 kinase , Grb 2 , and Csk . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
We show that phospholipase C gamma 1 , Ras GTPase activating protein , the p 85 subunit of phosphatidylinositol 3 ' kinase , Shc , Grb 2 , Vav , Fyn , and Src are components of the Flk2 / Flt3 signal transduction pathway . ^^^ In addition , we demonstrate that phospholipase C gamma 1 , the p 85 subunit of phosphatidylinositol 3 ' kinase , Shc , Grb 2 , and Src family tyrosine kinases , but not Ras GTPase activating protein , Vav , or Nck , physically associate with the Flk2 / Flt3 cytoplasmic domain . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
On the other hand , kinase activation of Src and Raf 1 , phosphorylation of protein tyrosine phosphatase 1D / Syp and Shc , and complex formation with Grb 2 were greatly diminished by suramin . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
Protein binding assays with two Grb 2 mutants , Grb2 / P49L and Grb2 / G203R , which correspond to the loss of function mutants in the Caenorhabditis elegans sem 5 , demonstrated that the dystroglycan Grb 2 association is through beta dystroglycan C terminal proline rich domains and Grb 2 Src homology 3 domains . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
Significantly , all the insulin induced tyrosine phosphorylation sites identified in rat IRS 1 , including those responsible for binding to the Src homology domains of phosphatidylinositol ( PI ) 3 kinase , Syp and Grb 2 , are conserved in XIRS L . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
Association of p 62 , a multifunctional SH 2 and SH 3 domain binding protein , with src family tyrosine kinases , Grb 2 , and phospholipase C gamma 1 . src family tyrosine kinases contain two noncatalytic domains termed src homology 3 ( SH 3 ) and SH 2 domains . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
Therefore , DNAs from panels of rodent human hybrids carrying defined complements of human chromosomes were assayed for the presence of the cognate genes for NCK , SHC , and GRB 2 , three SH 2 or SH2 / SH3 ( Src homology 2 and 3 ) domain containing adapter proteins . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
Binding of Vav to Grb 2 through dimerization of Src homology 3 domains . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
Insulin receptor substrate 1 ( IRS 1 ) serves as the major immediate substrate of insulin / insulin like growth factor ( IGF ) 1 receptors and following tyrosine phosphorylation binds to specific Src homology 2 ( SH 2 ) domain containing proteins including the p 85 subunit of phosphatidylinositol ( PI ) 3 kinase and GRB 2 , a molecule believed to link IRS 1 to the Ras pathway . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
The activated FLT 4 tyrosine kinase domain was found to interact with the Src homology 2 domains of the SHC and GRB 2 adaptor proteins in vitro and with SHC in cells . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
Cytosolic Shc bound only GRB 2 , whereas , membrane associated Shc complexed with GRB 2 , the PDGF beta receptor , Src , and additional tyrosine phosphorylated proteins . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
Here we show that adhesion of murine NIH3T3 fibroblasts to fibronectin promotes SH 2 domain mediated association of the GRB 2 adaptor protein and the c Src protein tyrosine kinase ( PTK ) with FAK in vivo , and also results in activation of mitogen activated protein kinase ( MAPK ) . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
This report demonstrates that NPTY is required for interaction between MT and SHC protein , a Src homology 2 ( SH 2 ) containing protooncogene product implicated in activating Ras via association with GRB 2 protein . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
Interaction of Grb 2 via its Src homology 3 domains with synaptic proteins including synapsin 1 . ^^^ Grb 2 is a 25 kDa adaptor protein composed of a Src homology 2 ( SH 2 ) domain and two flanking Src homology 3 ( SH 3 ) domains . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
Also , our data show that EGF receptors are able to phosphorylate SHC , activate the exchange of guanine nucleotide on ras and phosphorylate MAPK by a mechanism that does not require receptor autophosphorylation sites and , therefore , the src homology 2 ( SH 2 ) : phosphotyrosine dependent interaction of SHC or GRB 2 with the EGF receptor . . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
Numerous recent studies have implicated the src homology 2 and 3 domain containing protein , Grb 2 , in coupling protein tyrosine kinase signaling pathways with the Ras signaling pathway . ^^^ Through the use of mutated Grb 2 fusion proteins , we demonstrate that pp 116 binds the amino terminal src homology 3 domain of Grb 2 , the same domain of Grb 2 thought to be primarily responsible for its interaction with SOS . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
Tyrosine phosphorylation of this substrate results in its being bound by various proteins containing src homology 2 ( SH 2 ) sites , including a phosphatidylinositol 3 kinase and a ras activator complex containing GRB 2 and son of sevenless ( SOS ) 1 . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
Such polypeptides include Grb 2 , which comprises a single Src homology 2 ( SH 2 ) domain flanked by two SH 3 domains , and the 66 , 52 and 46 kDa Shc proteins which possess an SH 2 domain in their carboxy terminus . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
In this study we examine the ability of tyrosine phosphorylated proteins from Jurkat T cells stimulated by CD 2 or T cell receptor CD 3 to interact with the src homology 2 or src homology 3 domains from eight different proteins involved in signal transduction in lymphocytes : Vav , Shc , Nck , phosphatidylinositol 3 kinase , phospholipase C gamma 1 , Ras GTPase activating protein , c Crk and Grb 2 . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
Insulin promotes the formation of a complex between tyrosine phosphorylated IRS 1 and several proteins including phosphoinositide ( PI ) 3 kinase , a heterodimer consisting of regulatory 85 kDa ( p 85 ) and catalytic 110 kDa ( p 110 ) subunits , GRB 2 and Syp via the Src homology region 2 ( SH 2 ) domains . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
Growth factor receptor tyrosine kinases can form stable associations with intracellular proteins that contain src homology ( SH ) 2 domains , including two proteins , Shc and Grb 2 , that are thought to lie upstream from the ras protooncogene in a signaling cascade . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
This interaction was mediated by the carboxyl terminal domain of hSos 1 and the Src homology 3 ( SH 3 ) domains of GRB 2 . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
In fibroblasts over expressing insulin receptors we observed that two tyrosine phosphorylated proteins interact with Grb 2 and with a mutant of Grb 2 , which lacks the Src homology 2 ( SH 2 ) domain , indicating that these proteins associate with the SH 3 domains of Grb 2 . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
The Src homology ( SH ) 2 / SH3 domain containing protein Grb 2 and the oncoprotein Shc have been implicated in a highly conserved mechanism that regulates p21ras activation . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
We specifically evaluated whether these compounds affected 2 recently discovered proteins involved in controlling ras function : Shc , which is tyrosine phosphorylated by src and activated growth factor receptors , and Grb 2 , which mediates signal transduction from activated growth factor receptors through ras . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
While Sos binds to both Grb 2 Src homology 3 ( SH 3 ) domains , Vav was found to associate selectively with the carboxyl terminal SH 3 domain , while C3G bound selectively to the amino terminal SH 3 domain of bacterially expressed Grb 2 . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
We and others have recently identified Cbl , the protein product of the c cbl protooncogene , as an early tyrosine kinase substrate upon T cell activation and have shown that Cbl forms in vivo complexes with Src family tyrosine kinases , Grb 2 adaptor protein , and the p 85 subunit of PI 3 kinase . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
Distinct ligand preferences of Src homology 3 domains from Src , Yes , Abl , Cortactin , p53bp2 , PLCgamma , Crk , and Grb 2 . ^^^ Specifically , we have found that the Src SH 3 domain selects peptides sharing the consensus motif LXXRPLPXpsiP , whereas Yes SH 3 selects psiXXRPLPXLP , Abl SH 3 selects PPXthetaXPPPpsiP , Cortactin SH 3 selects +PPpsiPXKPXWL , p53bp2 SH 3 selects RPXpsiPpsiR+SXP , PLCgamma SH 3 selects PPVPPRPXXTL , Crk N terminal SH 3 selects psiPpsiLPpsiK , and Grb 2 N terminal SH 3 selects +thetaDXPLPXLP ( where psi , theta , and + represent aliphatic , aromatic , and basic residues , respectively ) . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
Cbl was basally associated with the adaptor protein growth factor receptor binding protein 2 ( Grb 2 ) , and this interaction was further enhanced by EGF stimulation ; however , the interaction was entirely mediated via the Grb 2 Src homology 3 ( SH 3 ) domains , suggesting that binding of Grb 2 SH2 domain to EGF receptor provides one mechanism of Cbl ' s association with the EGF receptor . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
Analysis of tyrosine phosphorylated RAFTK from adherent transfected COS cells showed that the Src homology 2 ( SH 2 ) domains of the Src and Fyn protein kinases as well as the Grb 2 adaptor protein were able to specifically associate with RAFTK . ^^^ These data suggest that RAFTK is a novel member of the FAK family , that it localizes to focal adhesion like structures in CMK megakaryocytic cells , that it participates in integrinmediated signaling pathways in megakaryocytes , and that it is able to associate with the tyrosine kinases Src and Fyn as well as the adaptor protein Grb 2 via SH 2 phosphotyrosine interactions . . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
The Src homology 2 ( SH 2 ) and SH 3 domain containing adaptor protein GRB 2 and the SH 2 domain containing protein tyrosine phosphatase 1D ( PTP1D , also called SHPTP 2 , PTP2C , SHPTP 3 , Syp , or SHP 2 ) function as positive mediators of growth factor induced mitogenesis . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
RDPE 6 showed binding to GST fusion proteins of both the intact Src kinase and an SH 2 SH3 construct but did not bind to the Src SH 2 domain or to the adaptor protein Grb 2 . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
We showed previously that the c Src nonreceptor PTK and the Grb 2 SH2 / SH3 adaptor protein bound directly to FAK after fibronectin stimulation ( D . ^^^ Here , we present evidence that c Src association with FAK is required for Grb 2 binding to FAK . ^^^ Using epitope tagged FAK constructs , transiently expressed in human 293 cells , we determined the effect of site directed mutations on c Src and Grb 2 binding to FAK . ^^^ Mutation of FAK Tyr 925 disrupted Grb 2 binding , whereas mutation of the c Src binding site on FAK ( Tyr 397 ) disrupted both c Src and Grb 2 binding to FAK in vivo . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
We propose that Pyk 2 acts with Src to link Gi and Gq coupled receptors with Grb 2 and Sos to activate the MAP kinase signalling pathway in PC 12 cells . . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
In contrast , Shc , phosphatidylinositol 3 kinase ( Pl3K ) , Grb 2 , and Ras GTPase activating protein ( GAP ) bound with different affinities to the bis or monophosphorylated peptides , while the Src family protein tyrosine kinase ( PTK ) Fyn did not bind specifically to any of the tested peptides . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
In this work , we show binding of WASP to the Src homology 3 domains of Btk , Itk , Tec , Grb 2 , and phospholipase C gamma , which suggests a function for WASP in lymphoid cell signaling . . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
Formation of this complex , which contains no detectable levels of Sos , is known to depend on a C terminal phosphorylated tyrosine residue ( Tyr 798 ) in RPTPalpha and on the Src homology ( SH ) 2 domain in Grb 2 ( , ) . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
The molecules discussed include Src family kinases , Syk , Grb 2 adaptor protein , and Tec family kinases Bmx and Btk . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
The results are discussed in relation to potential roles for the vertebrate homologues of Drk and Csw ( Grb 2 and SHP 2 , respectively ) in the transformation of fibroblasts by vertebrate Src . . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
Purified amino terminal Src homology 2 ( SH 2 ) domains of GAP , PLCgamma 1 and the p85alpha subunit of PI 3 kinase , as well as the carboxy terminal SH 2 domain of the latter protein and the unique SH 2 domain of Grb 2 , were injected into full grown , stage 6 Xenopus laevis oocytes . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
Previously identified Src sites include catalytic domain tyrosines 576 and 577 , important for maximal FAK kinase activity , and tyrosine 925 , which permits an SH 2 mediated association with Grb 2 . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
Following binding of platelet derived growth factor ( PDGF ) , the PDGF alpha receptor ( alphaPDGFR ) becomes tyrosine phosphorylated and associates with a number of signal transduction molecules , including phospholipase Cgamma 1 ( PLCgamma 1 ) , phosphatidylinositol 3 kinase ( PI3K ) , the phosphotyrosine phosphatase SHP 2 , Grb 2 , and Src . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
The association of p 135 and Grb 2 was dependent on an intact Src homology 3 domain and furthermore , was shown to preferentially interact with the N terminal Src homology 3 domain of Grb 2 , which is similar to the interaction of mSos and Grb 2 in other cell types . p 135 was not recognized by antibodies against several known Grb 2 binding proteins and thus may be a novel Grb 2 binding protein . . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
Herein , we demonstrate that pp60c src can utilize GRB 2 as a substrate . ^^^ Incubation of native or recombinant GRB 2 with purified pp60c src provided additional support for pp60c src as the kinase for GRB 2 . ^^^ Deletion mutants of GRB 2 demonstrated that pp60c src phosphorylated GRB 2 on a tyrosine residue ( residue 160 ) located between the SH 2 domain and carboxyl terminal SH 3 domain . ^^^ Mutation of tyrosine 160 to phenylalanine abolished phosphorylation of GRB 2 by pp60c src . ^^^ NIH 3T3 fibroblasts overexpressing pp60v src contained high levels of phosphorylated GRB 2 . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
These data suggest that Gbetagamma subunit mediated activation of Src family nonreceptor tyrosine kinases can account for the Gi coupled receptor mediated tyrosine phosphorylation events that direct recruitment of the Shc and Grb 2 adapter proteins to the membrane . . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
Fibronectin stimulated signaling from a focal adhesion kinase c Src complex : involvement of the Grb 2 , p130cas , and Nck adaptor proteins . ^^^ FAK autophosphorylation at Tyr 397 promotes Src homology 2 ( SH 2 ) domain binding of Src family PTKs , and c Src phosphorylation of FAK at Tyr 925 creates an SH 2 binding site for the Grb 2 SH2 SH 3 adaptor protein . ^^^ Stable expression of residues 1 to 298 of Src ( Src 1 298 , which encompass the SH 3 and SH 2 domains of c Src ) in the Src cells blocked Grb 2 binding to FAK ; but surprisingly , Src 1 298 expression also resulted in elevated p130cas P . ^^^ Tyr ) and Grb 2 binding to FAK were reduced , whereas the tyrosine phosphorylation of another signaling protein , p130cas , was not detected in the Src cells . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
In this respect , it shares some aspects of signaling with growth factor and cytokine receptors , including activation of phospholipase C gamma , src , and ras ; association of shc with grb 2 ; and stimulation of the Jak / STAT pathway . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
Subsequent recruitment of proteins containing Src homology 2 domains , including Grb 2 and c Crk , to the complex is likely to trigger adhesion induced cellular responses , including changes to the actin cytoskeleton and activation of the Ras MAP kinase pathway . . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
Insulin induced tyrosine phosphorylation of a M ( r ) 70 , 000 protein revealed by association with the Src homology 2 ( SH 2 ) and SH 3 domains of p120GAP and Grb 2 . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
To investigate the mechanisms by which FAK , c Src , and Grb 2 function in FN stimulated signaling events to ERK 2 , we expressed wild type and mutant forms of FAK in human 293 epithelial cells by transient transfection . ^^^ Focal adhesion kinase ( FAK ) associates with integrin receptors , and FN stimulated phosphorylation of FAK at Tyr 397 and Tyr 925 promotes the binding of Src family PTKs and Grb 2 , respectively . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
Using different far Western blot analyses and coimmunoprecipitation assays , receptor binding of PLCgamma and p 85 proteins as well as GRB 2 , c src and lck was examined in vitro and in vivo . ^^^ Competitive inhibition of substrate binding and mutagenesis experiments with EGFR / Ufo constructs revealed C terminal tyrosine 821 ( EILpYVNMDEG ) as a docking site for multiple effectors , namely PLCgamma , p 85 proteins , GRB 2 , c src and lck . ^^^ Moreover , our in vivo data indicate that further direct or indirect binding sites for PLCgamma , GRB 2 , c src and lck on the human Ufo receptor may exist . . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
The product of the c cbl proto oncogene ( c Cbl ) is a 120 kDa protein harboring several docking sites for Src homology 2 ( SH 2 ) domain containing proteins and proline rich regions that have been shown to allow its constitutive association with the SH 3 domains of Grb 2 . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
The binding is mediated by the Src homology 2 ( SH 2 ) domain of Grb 2 and the tyrosine phosphorylated delta subunit of the AChR . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
The mammalian members include Sam 68 , which is a target of Src , Fyn , and Grb 2 , and the newly cloned mouse quaking proteins ( qkI ) necessary in early embryogenesis and myelination . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
Consistent with this observation , pp 55 selectively binds to isolated SH 2 domains of Lck , Lyn , Src , and Fyn but not to the SH 2 domains of ZAP 70 , Syk , Shc , SLP 76 , Grb 2 , phosphatidylinositol 3 kinase , and c abl in vitro . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
The molecular adapter c Cbl is rapidly tyrosine phosphorylated following stimulation through the TCR and associates with Src homology domain 2 ( SH 2 ) / SH3 domain containing adapters such as Grb 2 , Crk , and Crk L , which interact with guanine nucleotide exchange factors specific for the Ras family . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
Gi mediated activation of the Ras / MAP kinase pathway involves a 100 kDa tyrosine phosphorylated Grb 2 SH3 binding protein , but not Src nor Shc . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
The human proto oncogene product c Cbl and a similar protein in Caenorhabditis elegans ( Sli 1 ) contain a proline rich COOH terminal region that binds Src homology 3 ( SH 3 ) domains of proteins such as the adapter Grb 2 . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
Activation of ERK 1 and 2 may involve the Src homology 2 containing proteins Shc and Grb 2 . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
Coupling of the murine protein tyrosine phosphatase PEST to the epidermal growth factor ( EGF ) receptor through a Src homology 3 ( SH 3 ) domain mediated association with Grb 2 . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
Here it is reported that ET 1 promotes the formation of stress fibers and focal adhesions and the tyrosine phosphorylation of focal adhesion kinase ( FAK ) and paxillin , as well as Src activation and association of phosphorylated FAK with Grb 2 . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
The SH3p4 / Sh3p8 / SH3p13 protein family : binding partners for synaptojanin and dynamin via a Grb 2 like Src homology 3 domain . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
In the case of polypeptide growth factors such as the epidermal growth factor ( EGF ) and platelet derived growth factor ( PDGF ) , the steps leading to activation of MAPK require the function of the adaptor protein Grb 2 ( growth factor receptor binding protein 2 ) , which can bind either directly or indirectly via its Src homology 2 domain to activated receptor tyrosine kinases . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
The GTPase activity of neuronal specific dynamin 1 is stimulated by microtubules , negatively charged phospholipid vesicles , and Src homology 3 containing proteins , including Grb 2 . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
Using affinity precipitation , WASP was found to bind to Src SH 3 containing proteins Fyn , Lck , PLC gamma , and Grb 2 , and mutated WASP , if expressed , was able to bind to Fyn glutathione S transferase ( GST ) fusion protein . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
This complex consists of Src homologous collagen protein ( Shc ) , growth factor receptor binding protein 2 ( Grb 2 ) , son of sevenless ( Sos ) , and a novel tyrosine phosphorylated form of the cytoskeletal regulatory protein caldesmon . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
Nonphosphorylated peptide ligands for the Grb 2 Src homology 2 domain . ^^^ Critical intracellular signals in normal and malignant cells are transmitted by the adaptor protein Grb 2 by means of its Src homology 2 ( SH 2 ) domain , which binds to phosphotyrosyl ( pTyr ) residues generated by the activation of tyrosine kinases . ^^^ Using phage display , an 11 amino acid cyclic peptide , G 1 , has been identified that binds to the Grb 2 SH2 domain but not the src SH 2 domain . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
Protein binding assays demonstrated that among the major src homology 3 proteins known to bind to the proline rich region of synaptojanin 1 , Grb 2 , amphiphysin , and members of SH3p4 / 8 / 13 protein family , only Grb 2 bound to that of synaptojanin 2 . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
Activation and autophosphorylation of FAK lead to its binding to a number of intracellular signaling molecules , including Src , Grb 2 and PI 3 kinase . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
The Src family kinase Hck interacts with Bcr Abl by a kinase independent mechanism and phosphorylates the Grb 2 binding site of Bcr . bcr abl , the oncogene causing chronic myeloid leukemia , encodes a fusion protein with constitutively active tyrosine kinase and transforming capacity in hematopoietic cells . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
Src association with FAK may also lead to its phosphorylation of other sites on FAK , including a binding site for Grb 2 . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
In this report , we have examined the role of FAK association with Grb 2 and p 130 ( Cas ) , two downstream events of the FAK / Src complex that could mediate integrin stimulated activation of extracellular signal regulated kinases ( Erks ) . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
We established that , in unstimulated lymphocytes , the Src homology 2 ( SH 2 ) and SH 3 domain containing protein Grb 2 and the p 85 subunit of phosphatidylinositol 3 kinase , associate constitutively with Cbl via their SH 3 domains . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
A direct interaction between Sam 68 and the two src kinases involved in T cell activation , p 59 ( fyn ) and p 56 ( lck ) , as well as a partnership of Sam 68 with various key downstream signaling molecules , like phospholipase Cgamma 1 and Grb 2 , has been shown . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
In cardiac fibroblasts , Ang 2 activates ERKs through a pathway including the Gbetagamma subunit of Gi protein , tyrosine kinases including Src family tyrosine kinases , Shc , Grb 2 , Ras , and Raf 1 kinase , whereas Gq and protein kinase C are important in cardiac myocytes . . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
Grb 2 is an adaptor molecule comprising one Src homology ( SH ) 2 and two SH 3 domains . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
Studies with GST Grb 2 fusion proteins demonstrated that the association was specific for the Src homology 2 domain of Grb 2 . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
IRS 4 was found to be associated with two src homology 2 ( SH 2 ) domain containing proteins , phosphatidylinositol 3 kinase and Grb 2 , the adaptor to the guanine nucleotide exchange factor for Ras . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
Furthermore , we identify two tyrosine residues that are subject to phosphorylation in response to muscarinic signaling and show that this phosphorylation induces two cytosolic proteins , c Src and Grb 2 , to bind to PYK 2 . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
Multiple Grb 2 mediated integrin stimulated signaling pathways to ERK2 / mitogen activated protein kinase : summation of both c Src and focal adhesion kinase initiated tyrosine phosphorylation events . ^^^ FN stimulation of NIH 3T3 fibroblasts promotes c Src and FAK association in the Triton insoluble cell fraction , and the time course of FN stimulated ERK 2 activation paralleled that of Grb 2 binding to FAK at Tyr 925 and Grb 2 binding to Shc . ^^^ Interestingly , c Src in vitro phosphorylation of Shc promoted Grb 2 binding to both wild type and Phe 317 Shc . ^^^ Treatment of fibroblasts with protein kinase C inhibitors or with the PTK inhibitor herbimycin A or PP 1 resulted in reduced Src PTK activity , no Grb 2 binding to FAK , and lowered levels of ERK 2 activation . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
In this report we characterize an as yet unidentified 97 kDa protein that is a major tyrosine kinase substrate in IL 2 stimulated cells . pp 97 was found to associate with the p85 . p110 phosphatidylinositol 3 kinase complex , the Src homology 2 ( SH 2 ) domain containing tyrosine phosphatase SHP 2 , and the adaptor molecules CrkL and Grb 2 . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
We find that the Src homology 3 domains of Grb 2 directly associate , in vitro and in vivo , with murine hemopoietic specific protein 1 ( HS 1 ) , a protein identical to Lck binding protein 1 . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
Thus , we examined the interactions of various SH 2 containing molecules like PLC gamma 1 , rasGAP , p 85 subunit of PI 3 kinase , Src , Fyn , Crk , Nck , Grb 2 and Shc with HEK 2 using in vitro binding assays , immunoprecipitations and yeast Two Hybrid assays . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
Cholesterol depleted caveolae had a reduced amount of several key protein components of the MAP kinase complex , including Ras , Grb 2 , Erk 2 , and Src . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
Grb 2 forms an inducible protein complex with CD 28 through a Src homology 3 domain proline interaction . ^^^ Here we show that CD 28 is capable of binding the Src homology 3 ( SH 3 ) domains of several proteins , including Grb 2 . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
ErbB 2 binding to peptides containing the Src homology 2 domain of Grb 2 or p 85 and the phosphotyrosine binding domain of Shc varied according to the mode of receptor activation . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
Under these conditions , insulin induced Ras activation was also abolished , and Grb 2 association with the Src and collogen homologous ( Shc ) molecule was inhibited without inhibition of the tyrosine phosphorylation of Shc . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
In addition , 14 , 15 EET SI administration stimulated tyrosine phosphorylation of src homologous and collagen like protein ( SHC ) and association of SHC with both growth factor receptor binding protein ( GRB 2 ) and EGFR . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
Phosphorylation of Try 350 / 354 creates a Src homology 2 ( SH 2 ) domain on the beta 2 adrenergic receptor and the binding of adaptor protein Grb 2 to this SH 2 domain of the beta adrenergic receptor takes place in an insulin dependent manner . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
Insulin administration in vitro and in vivo stimulates phosphorylation of Tyr 350 of the beta adrenergic receptor , creating an Src homology 2 domain available for binding of the adaptor molecule Grb 2 . ^^^ Insulin also stimulates association of phosphatidylinositol 3 kinase and dynamin , via the Src homology 3 domain of Grb 2 . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
Both the c Src and c Fyn tyrosine kinases are tyrosine phosphorylated and activated by cellular hGH stimulation and form part of the multiprotein signaling complex as does tensin , paxillin , IRS 1 , the p 85 subunit of phosphatidylinositol 3 kinase , C3G , SHC , Grb 2 , and Sos 1 . c Cbl and Nck are also tyrosine phosphorylated by cellular stimulation with hGH and associate with the p 130 ( Cas ) CrkII complex . c Jun N terminal kinase / stress activated protein kinase ( JNK / SAPK ) is activated in response to hGH in accordance with the formation of the abovementioned signaling complex , and hGH stimulated JNK / SAPK activity is increased in CrkII overexpressing NIH3T3 cells compared with vector transfected NIH3T3 cells . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
The analysis of Grb2 / c Cbl interaction demonstrated that , in both acute leukemia and CML blasts , c Cbl was stably complexed with the N terminal Src homology ( SH ) 3 domain of Grb 2 and , in blasts from ALL patients , with the Grb 2 SH2 domain . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
In conclusion , our results demonstrate that Gi enables the PDGFR to signal more efficiently to p42 / p44 MAPK , and this appears to be achieved through the regulation of c Src and Grb 2 / PI3K , which are intermediates in the p42 / p44 MAPK cascade . . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
In cardiac fibroblasts , angiotensin 2 activates ERKs through a pathway including the Gbetagamma subunit of Gi protein , Src family tyrosine kinases , Shc , Grb 2 and Ras , whereas Gq and protein kinase C are important in cardiac myocytes . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
Development of Grb 2 Src homology 2 ( SH 2 ) domain binding inhibitors has important implications for treatment of a variety of diseases , including several cancers . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
FAK interacts with a number of signaling and cytoskeletal proteins , including Src , phosphatidylinositol 3 kinase , Grb 2 , p130Cas and paxillin . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
Association of Shc , Cbl , Grb 2 , and Sos following treatment with 2 , 3 , 7 , 8 tetrachlorodibenzo p dioxin in primary rat hepatocytes . c Src kinases and p 21 Ras are known to be implicated in 2 , 3 , 7 , 8 tetrachlorodibenzo p dioxin ( TCDD ) mediated signal transduction . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
Although a selective EGFR kinase inhibitor completely abolished Ang 2 induced recruitment of Grb 2 to EGFR and markedly attenuated Ang 2 induced ERK activation , it had no effect on Ang 2 induced PYK 2 tyrosine phosphorylation or its association with c Src and Grb 2 . ^^^ These data suggest that the AT 1 receptor uses Ca2+ dependent PYK 2 to activate c Src , thereby leading to EGFR transactivation , which preponderantly recruits Grb 2 in rat VSMC . . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
Gads contains a Src homology 2 ( SH 2 ) domain , which has previously been shown to have a similar binding specificity to that of Grb 2 . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
These results demonstrate that S1P activates c Src and Grb 2 PI 3K ( intermediates in the p42 / p44 MAPK cascade ) via a PTX sensitive mechanism . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
Furthermore , we show that microinjected Src homology 2 domains from either Grb 2 or SHP 2 blocked the transforming activity of the env sea protein . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
In cardiac fibroblasts , Ang 2 activated MAP kinase through a pathway including the Gbetagamma subunit of Gi protein , Src , Shc , Grb 2 , and Ras , while Gq and protein kinase C were important in cardiac myocytes . . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
SLM 1 bound the SH 2 and SH 3 domains of p 59 ( fyn ) , Grb 2 , phospholipase Cgamma 1 ( PLCgamma 1 ) , and / or p 120 ( rasGAP ) , suggesting it may function as a multifunctional adapter protein for Src during mitosis . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
Mutation of Tyr 881 , a putative Src kinase phosphorylation site predicted to bind Grb 2 , had little effect on CADTK regulation . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
Following earlier work on cystine bridged peptides , cyclic phosphopeptides containing nonreducible mimics of cystine were synthesized that show high affinity and specificity toward the Src homology ( SH 2 ) domain of the growth factor receptor binding protein ( Grb 2 ) . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
Consistent with this model , FRS 2 binds Grb 2 , Crk , the SH 2 domain containing tyrosine phosphatase SH PTP 2 , the cyclin dependent kinase substrate p 13 ( suc 1 ) , and the Src homology 3 ( SH 3 ) domain of Src , providing a functional link between TrkA , cell cycle , and multiple NGF signaling effectors . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
The src homology domain 2 containing inositol phosphatase SHIP forms a ternary complex with Shc and Grb 2 in antigen receptor stimulated B lymphocytes . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
Pertussis toxin , PP 1 ( a selective Src family kinase inhibitor ) , or overexpression of a catalytically inactive mutant of Fyn blocked the UK 14304 or LPA stimulated activity of SHP 2 , SHP 2 tyrosine phosphorylation , and SHP 2 association with Grb 2 . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
We developed scintillation proximity assays ( SPA ) to discover compounds which inhibit phosphopeptide binding to Src homology 2 ( SH 2 ) domain proteins Grb 2 and Syk . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
A small pool of CSF 1R formed a multimeric complex with phosphatidylinositol 3 kinase ( PI 3 kinase ) , SHP 1 , Grb 2 , Shc , c Src , Cbl , and a significant number of tyrosine phosphorylated proteins in CSF 1 stimulated cells . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
The latter sequences mediate the binding to the SH 3 domains of p 130 ( Cas ) , Src family kinases , p 85 subunit of phosphatidylinositol 3 kinase , and Grb 2 . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
Activation of p 125 ( FAK ) was accompanied by its increased association with adapter proteins GRB 2 , Shc , and nonreceptor type tyrosine kinase p 60 ( c src ) . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
Finally , the structure of Grb 2 SH2 domain was compared with those of the complexes of Src and phospholipase C gamma 1 with their cognate peptides , showing that the specific conformation of the peptide was required for binding to the SH 2 domains . . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
It is shown that the association occurs through the Src homology 2 ( SH 2 ) domains of Grb 2 and Grb 7 . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
Previous work in our laboratory established that Bcr is a major transformation related substrate for the 5 Fps tyrosine kinase , and tyrosine phosphorylation of Bcr induces Bcr Grb 2 / SOS association in vivo through the Src homology 2 ( SH 2 ) domain of Grb 2 . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
In cardiac fibroblasts AngII activated MAP kinase via G beta gamma subunit of Gi , Src , Shc , Grb 2 , and Ras , whereas Gq and protein kinase C were critical in cardiomyocytes . . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
This series of compounds displays an unprecedented level of selectivity over the closely related tyrosine kinase Syk , as well as other SH 2 containing proteins such as Src and Grb 2 . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
The hormone mediates these effects by activation of signaling pathways which utilize , 1 ) adaptor molecules such as the insulin receptor substrates ( IRS ) , the Src and collagen homologs ( Shc ) , and the growth factor receptor binding protein 2 ( Grb 2 ) ; 2 ) lipid kinases such as phosphatidylinositol 3 kinase ( PI 3 Kinase ) ; 3 ) small G proteins ; and 4 ) serine , threonine , and tyrosine kinases . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
Tyrosine phosphorylation of Shc in response to B cell Ag receptor ( BCR ) engagement creates binding sites for the Src homology 2 ( SH 2 ) domain of Grb 2 . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
High affinity IgG receptor activation of Src family kinases is required for modulation of the Shc Grb 2 Sos complex and the downstream activation of the nicotinamide adenine dinucleotide phosphate ( reduced ) oxidase . ^^^ The Src family tyrosine kinase inhibitor , PP 1 , was shown to completely inhibit Shc tyrosine phosphorylation , the Shc Grb 2 interaction , and the FcgammaR induced respiratory burst . ^^^ Our results provide the first evidence that the upstream activation of Src kinases is required for the modulation of the Shc Grb 2 interaction and the myeloid NADPH oxidase response . . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
In particular , considerable research has been performed on Src , PI 3 kinase , Grb 2 and more recently , Lck . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
Overlay blots with recombinant Src homology 2 domains of IRS 1 adaptor proteins showed that the loss of IRS 1 binding of Crk , GRB 2 , SHP 2 , and the p 85 subunit of phosphatidylinositol 3 ' kinase paralleled the rate of overall IRS 1 dephosphorylation . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
Here , we show that hypoxia induced tyrosine phosphorylation of p 125 ( FAK ) and paxillin and that hypoxia induced activation of p 125 ( FAK ) was accompanied by its increased association with adapter proteins Shc and GRB 2 , and non receptor type tyrosine kinase p 60 ( c src ) . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
The SH 3 domains of c Src , phosphatidylinositol 3 OH kinase , phospholipase C gamma and Grb 2 protein ( containing two SH 3 domains ) , but not other SH 3 domains tested , were capable of binding Sam 68 in vitro . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
We found that CSR induced the activation of FAK and Src and the association of FAK and Shc , a signaling molecule linking growth factor receptor tyrosine kinase and Grb 2 . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
AP 1510 treatment induced tyrosine phosphorylation of ErbB 1 and ErbB 2 homodimers and recruitment of Src homology 2 domain containing proteins ( Shc and Grb 2 ) . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
Coexpression of Cas with 5 Src enhanced the association of Grb 2 with the adapter protein Shc and the protein tyrosine phosphatase Shp 2 ; coexpression of Shc or Shp 2 mutants significantly reduced SRE activation by Cas and 5 Src . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
In cardiac fibroblasts AngII activated MAP kinases through a pathway including the Gbetagamma subunit of Gi protein , Src , Shc , Grb 2 , and Ras , while Gq and PKC activation was necessary in cardiac myocytes . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
It thereby activates Src , and PTPalpha overexpression neoplastically transforms NIH 3T3 cells . pTyr 789 in PTPalpha is constitutively phosphorylated and binds Grb 2 , an interaction that may inhibit PTPalpha activity . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
A central proline rich motif associated with the Src homology ( SH ) 2 / SH3 containing adapter proteins Grb 2 and Nck in vivo , whereas a pleckstrin homology ( PH ) domain was located at the GEF C terminus . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
In cardiac fibroblasts , angiotensin 2 activated ERK via the G ( beta ) gamma subunit of Gi , Src , Shc , Grb 2 , and Ras , whereas Gq and protein kinase C were critical in cardiomyocytes . . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
A proline rich fragment of the NR2D subunit ( 2D ( 866 1064 ) ) bound to the Abl SH 3 domain but not to the SH 3 domains from Src , Fyn , Grb 2 , GAP , or phospholipase C gamma ( PLCgamma ) . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
All neuronal MAP 2 isoforms bound specifically to the SH 3 domains of c Src and Grb 2 in an in vitro glutathione S transferase SH 3 pull down assay . ^^^ Regulated association of microtubule associated protein 2 ( MAP 2 ) with Src and Grb 2 : evidence for MAP 2 as a scaffolding protein . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
Subsequent analysis revealed that Src and Syk / ZAP 70 tyrosine kinases and the adaptor proteins LAT , SLP 76 , BLNK , Grb 2 , and Grap are involved in HPK 1 activation . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
Upon T cell activation , LAT becomes highly phosphorylated on tyrosine residues , and Grb 2 , Gads , and phospholipase C ( PLC ) gamma 1 bind LAT via Src homology 2 domains . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
Our experiments demonstrate that BLNK , a recently identified adaptor molecule predominantly expressed in B cells , is associated with the CD 72 complex via the Src homology 3 domain ( s ) of Grb 2 in the cell line after membrane IgM ( mIgM ) engagement . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
SP induced formation of an activated EGFR complex containing the adapter proteins SHC and Grb 2 , but not c Src . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
Transfection of dominant negative Src constructs into F 4 cells had no effect on Grb 2 binding or PLC gamma phosphorylation . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
Recently , synapsin 1 has been found to bind the Src homology 3 ( SH 3 ) domains of Grb 2 and c Src . ^^^ The highest binding was observed with SH 3 domains of c Src , phospholipase C gamma , p 85 subunit of phosphatidylinositol 3 kinase , full length and NH ( 2 ) terminal Grb 2 , whereas binding was moderate with the SH 3 domains of amphiphysins I / II , Crk , alpha spectrin , and NADPH oxidase factor p 47 ( phox ) and negligible with the SH 3 domains of p 21 ( ras ) GTPase activating protein and COOH terminal Grb 2 . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
Recent advances in the design and synthesis of SH 2 inhibitors of Src , Grb 2 and ZAP 70 . ^^^ A perspective is offered on the recent development of Src homology 2 ( SH 2 ) antagonists of Src , Grb 2 and ZAP 70 . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
In addition , independent of PKC 12 ( S ) HETE increases tyrosine phosphorylation of Shc , and Grb 2 , stimulates association between Shc and Src , and increases the activity of Ras , via Src family kinases . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
Grb 2 protein is comprised of one SH 2 surrounded by two SH 3 domains and interacts by means of its Src homology ( SH 2 ) domain with phosphotyrosine residues of target proteins such as the epidermal growth factor ( EGF ) receptor or the Shc adaptor . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
These events were paralleled by c Src activation and binding to Fak and by an association of Grb 2 and p 85 subunit of phosphatidylinositol 3 kinase with Fak . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
Role of Grb 2 , phosphatidylinositol 3 kinase , Src tyrosine kinase , and phospholipase Cgamma . ^^^ The Ras activity was promoted by three upstream effectors , growth factor bound protein 2 ( Grb 2 ) , phosphatidylinositol 3 kinase ( PI3K ) and Src cytoplasmic kinase . ^^^ Ras activation was inhibited by a Grb 2 dominant negative form ( P49L ) , by PI3K inhibitors , including wortmannin , LY 294002 , the N SH 2 domain of p85alpha PI3K and by the SH 2 domain of Src . ^^^ The Grb 2 adaptor was recruited by the upstream Src homology 2 / alpha collagen related ( Shc ) effector , as the SH 2 Shc domain prevented the GVBD and the ERK 2 phosphorylation induced by FGF 1 . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
The interaction of activated epidermal growth factor receptor ( EGFR ) with the Src homology 2 ( SH 2 ) domain of the growth factor receptor binding protein Grb 2 initiates signaling through Ras and mitogen activated protein kinase ( MAP kinase ) [ 1 , 2 ] . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
Additionally , FAK was linked to the hypertrophic response as follows : 1 ) coimmunoprecipitation of beta1D and FAK was detected ; 2 ) FAK overexpression induced ANF luciferase ; 3 ) rapid and sustained phosphorylation of FAK was induced by alpha ( 1 ) adrenergic stimulation ; and 4 ) blunting of the alpha ( 1 ) adrenergically modulated hypertrophic response was caused by FAK mutants , which alter Grb 2 or Src binding , as well as by FAK related nonkinase , a dominant interfering FAK mutant . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
Finally , we provide evidence that the Src dependent association of FAK with Grb 2 and p 130 ( Cas ) are both required for the regulation of cell cycle progression by FAK . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
Meltrin alpha cytoplasmic domain interacts with SH 3 domains of Src and Grb 2 and is phosphorylated by 5 Src . ^^^ Src and Grb 2 in bovine brain extracts also bound to meltrin alpha cytoplasmic domain on affinity resin . ^^^ Furthermore , immunoprecipitation with a monoclonal antibody to meltrin alpha resulted in coprecipitation of Src and Grb 2 with meltrin alpha in cell extracts , suggesting that Src and Grb 2 are associated in vivo with meltrin alpha cytoplasmic domain . ^^^ This notion was also supported by the findings that exogenously expressed meltrin cytoplasmic domain coexisted with Src and Grb 2 on the membrane ruffles . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
Functional studies demonstrated that CR 2 activation induced , within a brief span of 2 min , tyrosine phosphorylation of nucleolin and its interaction with Src homology 2 domains of the p 85 subunit of PI 3 kinase and of 3BP2 and Grb 2 , but not with Src homology 2 domains of Fyn and Gap . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
It has been reported that c Src tyrosine kinase phosphorylates focal adhesion kinase at tyrosine 925 within focal adhesions , which in turn creates the binding site for Grb 2 , leading to MAPK activation . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
Co expression studies in human 293T cells showed that 5 Src could associate with and phosphorylate a Phe 397 FAK mutant at Tyr 925 promoting Grb 2 binding to FAK in suspended cells . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
Potent blockade of hepatocyte growth factor stimulated cell motility , matrix invasion and branching morphogenesis by antagonists of Grb 2 Src homology 2 domain interactions . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
At 1 min after ECS , the binding of Pyk 2 to Src and Grb 2 , and of Grb 2 to Ras increased . ^^^ These results suggested that ECS activates Pyk 2 , which then transmits the signal to MAPK cascade via Src , Grb 2 , and Ras in the rat hippocampus . . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
Role of Grb 2 , phosphatidylinositol 3 kinase , Src tyrosine kinase , and phospholipase Cgamma . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
Differential regulation of cell migration and cell cycle progression by FAK complexes with Src , PI3K , Grb 7 and Grb 2 in focal contacts . ^^^ We found that fusion of FAT to Src , the p 85 subunit of phosphatidylinositol 3 kinase , Grb 7 and Grb 2 resulted in the efficient focal adhesion targeting of these signaling molecules . ^^^ We showed that expression of Src FAT , p 85 FAT , or Grb 7 FAT , but not Grb 2 FAT , each stimulated cell migration . ^^^ In contrast , targeting of Grb 2 , but not Src , p 85 , or Grb 7 , to focal contacts increased cell cycle progression . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
The past two years have witnessed a number of significant advances in the design of SH 2 inhibitors of both Src and Grb 2 . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
These SH 3 ligands appeared to be functional as they facilitated interaction with the adaptor protein , Grb 2 , and the Src family tyrosine kinase , Fyn . . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
The new protein mainly binds to the proline rich region of mDia through its Src homology 3 domain and also binds to Grb 2 through its proline rich domain . ^^^ Our results suggest that this new protein regulates actin polymerization and cell adhesion turnover in the downstream portion of the Rho mDia pathway by interacting with Grb 2 and Src . . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
This includes the protein tyrosine kinases Src , Hck , and Fyn , the p85alpha regulatory subunit of phosphatidylinositol 3 kinase , phospholipase Cgamma , and the adaptor protein Grb 2 . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
Unlike SHP 1 , Grb 2 recruitment to CD 22 is not inhibited by specific doses of the Src family kinase specific inhibitor PP 1 . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
Antagonists of the Src homology 2 ( SH 2 ) domains of Grb 2 , Src , Lck and ZAP 70 . ^^^ Among the SH 2 domains pursued as drug discovery targets in the last few years are those of Grb 2 , Src , Lck and ZAP 70 . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
The latter effect probably abates Grb 2 inhibition of the phosphotyrosine displacement process that is required specifically for Src dephosphorylation and causes a mitotic increase in transient PTPalpha Src binding . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
E 2 induced phosphorylation of c Src is associated with increased tyrosine phosphorylation of Shc , increased association of Shc with Grb 2 , and induction of Ras , but not Rap 1 , activation . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
Growth factor receptor binding protein 2 ( Grb 2 ) plays a key role in signal transduction initiated by Bcr / Abl oncoproteins and growth factors , functioning as an adaptor protein through its Src homology 2 and 3 ( SH 2 and SH 3 ) domains . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
Their phosphotyrosine containing motifs become binding sites for Src homology 2 ( SH 2 ) domains on proteins such as SH 2 domain containing protein tyrosine phosphatase ( SHP ) 2 / Syp , growth factor receptor bound 2 protein , ( Grb 2 ) , and phosphatidyl inositol 3 kinase ( PI 3 kinase ) , which participate in activation of specific signaling cascades . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
We show here that the cytoplasmic domain of ADAM 15 , metargidin , can interact specifically with Src family protein tyrosine kinases ( PTKs ) and the adaptor protein Grb 2 in hematopoietic cells ( Jurkat , THP 1 , U 937 , and K 562 cell lines ) . ^^^ These data demonstrate selective , phosphorylation dependent interactions of ADAM 15 with Src family PTKs and Grb 2 , which highlight the potential for integration of ADAM functions and cellular signaling . . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
Phosphorylation of LAT creates binding sites for the Src homology 2 ( SH 2 ) domains of other proteins , including PLC gamma 1 , Grb 2 , Gads , Grap , 3BP2 , and Shb , and indirectly binds SOS , c Cbl , Vav , SLP 76 , and Itk . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
Increases of diastolic pressure from approximately 0 to approximately 15 mmHg rapidly increased FAK tyrosine phosphorylation ( maximum : 2 . 3 fold ) and binding to c Src ( maximum : 2 . 8 fold ) and Grb 2 ( maximum : 3 . 6 fold ) . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
This resulted in increased binding of another adaptor protein Grb 2 to the Src EGFR complex and the subsequent activation of Ras and MAPKs . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
During mitosis , the catalytic activity of protein tyrosine phosphatase ( PTP ) alpha is enhanced , and its inhibitory binding to Grb 2 , which specifically blocks Src dephosphorylation , is decreased . ^^^ This involves serine phosphorylation dependent differential modulation of the affinity of Tyr ( P ) 789 for the Src and Grb 2 SH2 domains . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
Pretreatment of AEC with Src homology 3 binding peptide , which blocks the interaction between Grb 2 and Sos , did not prevent DA activation of ERK . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
This ability of UCS15A was not restricted to Src SH 3 mediated protein protein interactions , since the drug was capable of disrupting the in vivo interactions of Sam 68 with other SH 3 domain containing proteins such as Grb 2 and PLCgamma . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
In order to study the role of Grb 2 in blood platelet responses , we used a peptide containing two proline rich sequences derived from Sos ( peptidimer ) , which binds to Grb 2 Src homology 3 domain ( SH 3 ) with a high affinity , and hence inhibits Grb 2 SH3 mediated protein interactions . ^^^ In thrombin activated platelets , the Grb 2 SH2 domain formed an association with the beta 3 subunit of the alphaIIb beta 3 integrin ( GPIIb IIIa ) , Shc , Syk , Src and SHP 1 ( SH 2 containing phosphotyrosine phosphatase 1 ) , whereas these associations did not occur after the engagement of the receptor for the Fc domain of IgG ( FcgammaRIIa ) or in resting platelets . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
As in other cells , Gab 2 was found to associate with several signaling molecules including Src homology 2 containing protein tyrosine phosphatase 2 , Grb 2 , Lyn , and phospholipase C gamma ( PLC gamma ) . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
We have investigated the regulation of Pyk 2 in human umbilical vein endothelial cells in response to GPCAs and show that ( 1 ) thrombin , a PAR 1 peptide , and histamine cause rapid concentration and time dependent phosphorylation on tyrosines 402 ( Src kinase binding site ) , 881 ( Grb 2 binding site ) , and 580 ( an autophosphorylation site ) , ( 2 ) thrombin stimulated phosphorylation is dependent on intracellular calcium and independent of PKC and PI 3 kinase , and ( 3 ) inhibition of Src kinases has no significant effect on thrombin stimulated phosphorylation , implying that tyrosine phosphorylation of Pyk 2 is independent of Src binding . . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
We demonstrate the value of this approach by the selection of peptide ligands that are either highly specific for the Grb 2 Src homology 2 ( SH 2 ) domain or that are cross reactive between a group of related SH 2 domains . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
This process resulting in the motility of intracellular vesicles at the tips of actin comets involved the recruitment of the Src homology 3 ( SH 3 ) SH 2 adaptor proteins Nck and Grb 2 as well as of WASP interacting protein ( WIP ) . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
Upon stimulation via the B or T cell receptors , LAX is rapidly phosphorylated by Src and Syk family tyrosine kinases and interacts with Grb 2 , Gads , and p 85 . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
Noteworthy are peptidomimetic and nonpeptide inhibitors of Src , Lck , Grb 2 , PI 3K , and Zap 70 from pioneering efforts that led to the first examples of cellularly and in vivo active SH 2 inhibitors . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
This allowed us to design mutants that selectively failed to associate with APS , while still associating with Src family members , SHP 2 and Grb 2 , respectively . . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
Stimulation of Kit by its ligand , stem cell factor activates several distinct early signaling pathways , including phospholipase C gamma , phosphatidylinositol 3 kinase , Src kinase , Grb 2 , and Grb 7 . ^^^ Combined stimulation with Epo also resulted in impaired cooperation between Src defective Kit mutant and Epo R and , to a lesser extent , with Kit mutants defective in the activation of phosphatidylinositol 3 kinase or Grb 2 . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
Endogenous FAK+ bound specifically to the SH 3 domains of c Src ( but not n Src ) , Fyn , Yes , phosphtidylinositol 3 kinase , amphiphysin 2 , amphiphysin 1 , phospholipase Cgamma and NH 2 terminal Grb 2 . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
Of particular interest is the observation that a highly conserved C terminal Class 2 polyproline motif within NS5A mediated association with the Src homology 3 domains of members of the Src family of tyrosine kinases and the mitogenic adaptor protein Grb 2 ( A . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
Insulin receptor phosphorylated IRS5 / DOK4 associates with RasGAP , Crk , Src , and Fyn , but not phosphatidylinositol 3 kinase p 85 , Grb 2 , SHP 2 , Nck , or phospholipase Cgamma Src homology 2 domains , and activates MAPK in cells . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
This tyrosine residue is found in a consensus binding motif ( YXN ) for the Src homology 2 domains of Grb 2 and Grb 7 ( where Grb stands for growth factor receptor bound protein ) . ^^^ Glutathione S transferase pull down assays showed that the interaction of G6f with both Grb 2 and Grb 7 is mediated through the Src homology 2 domains of these two proteins and is dependent on the phosphorylation of G6f . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
Here we demonstrate that activated EphB 1 recruits the adaptor proteins Grb 2 and p52Shc and promotes p52Shc and c Src tyrosine phosphorylation as well as MAPK / extracellular signal regulated kinase ( ERK ) activation . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
To further define the events linking the membrane distal tyrosine and JNK activation , the Src homology 2 domains of Shc , Grb 2 , and 3BP2 were shown to bind the full length GCSF R and a phosphopeptide encompassing the membrane distal tyrosine . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
The adaptor proteins Grb 2 , CrkII , and Nck 1 are composed of only a single Src homology 2 domain and some Src homology 3 domains , giving specificity to each signal transduction pathway . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
Activation of both PKB and Erk as well as survival in low serum containing media are all rescued by reexpression of Grb 2 containing mutations within the N terminal Src homology 3 ( SH 3 ) domain , but not by C terminal SH 3 domain mutants . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
Several papers have appeared in recent years on the discovery of new Grb 2 , Src , Syk , and Lck SH 2 domains binding antagonists . ^^^ As regards Grb 2 , Src and Lck SH 2 domains , rigidification of the starting high affinity binding peptides afforded derivatives with improved affinity ; cellular activity was achieved by modification of the side chains of these inhibitors . . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
In addition , Tom1L1 possesses several tyrosine motifs at the C terminal region that mediate interactions with members of the Src family kinases and other signaling proteins such as Grb 2 and p 85 . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
However , EGFR endocytosis was rescued in Grb 2 depleted cells by chimeric proteins consisting of the Src homology ( SH ) 2 domain of Grb 2 fused to c Cbl . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
Inhibitors of the Grb 2 and Src SH 2 domains have been extensively studied , with the aim of targeting the Ras pathway and osteoclastic bone resorption , respectively . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
Distinct ways for phosphopeptides to plug into Src and Grb 2 SH2 domains . ^^^ Binding of phosphotyrosine ( pY ) containing peptides to Src and Grb 2 SH2 domains was investigated using a surface plasmon resonance ( SPR ) based method . ^^^ The results for a pYVNV peptide binding to Grb 2 SH2 suggested a conformational change for Grb 2 SH2 upon binding , which is not observed for Src SH 2 . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
In a quest for molecules upstream of ShcA in this signaling , we found that CSR induced ShcA tyrosine phosphorylation , its association with Grb 2 , Erk activation , and uPA gene expression were all dependent on Rho kinase , p 38 mitogen activated protein kinase , and Src . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
In addition , we showed that active Src phosphorylates TrkA directly in vitro , and that Src associates with TrkA through Grb 2 after NGF stimulation . ^^^ These results suggest that constitutively active Src that associates with TrkA through Grb 2 after NGF stimulation participates in TrkA phosphorylation and in turn enhances the mitogen activated protein kinase signaling in SK N MC cells . . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
Of particular interest is the observation that a highly conserved C terminal polyproline motif in NS5A was able to interact with the Src homology 3 ( SH 3 ) domains of the adaptor protein Grb 2 . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
Furthermore , we show that this interaction is direct and that Grb 2 binds to phospho AbetaPP via its Src homology 2 region . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
To analyze the cell signaling interactions involving the adaptor protein Grb 2 , pBpa was incorporated in its Src homology 2 ( SH 2 ) domain . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
Gab 2 is persistently tyrosine phosphorylated in Ba / F3 cells expressing caSTAT 5 ( constitutively activated STAT 5 ) , independent of JAK 2 ( Janus kinase 2 ) activation where it interacts with STAT 5 , p 85 and Grb 2 , but not with Shp 2 [ SH 2 ( Src homology 2 ) domain containing tyrosine phosphatase ] proteins . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
For example , SH 3 domains from c Src , Grb 2 , and intersectin bound only to the C terminal half of dynamin 2 PRD but to both the N and C terminal portions of dynamin 1 PRD . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
We mapped this interaction showing that the Src homology 2 domain of Grb 2 and the tyrosine residue Y 606 in CD 229 are required for CD 229 Grb2 complex formation . ^^^ Moreover , a dominant negative form of Grb 2 ( containing only Src homology 2 domain ) impaired CD 229 endocytosis . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
To conduce these activities , the cytoplasmic C terminal region of this receptor acts as a docking site for multiple protein substrates , including Grb 2 , Gab 1 , STAT 3 , Shc , SHIP 1 and Src . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
This appears to occur through assembly of an EphB associated protein complex that includes focal adhesion kinase ( FAK ) , Src , Grb 2 , and paxillin and the subsequent activations of FAK , Src , paxillin , and RhoA . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
Knowledge of Grb 2 protein structure , its component Src homology domains and their respective structure function relationships has facilitated the rapid development of sophisticated drug candidates that can penetrate cells , bind Grb 2 with high affinity and potently antagonize Grb 2 signaling . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
The E76K mutation in the N terminal Src homology 2 domain increased interactions of mutant SHP 2 with Grb 2 , Gab 2 , and p 85 , leading to hyperactivation of IL 3 induced Erk and phosphatidylinositol 3 kinase ( PI3K ) pathways . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
However , it is not known whether oxidative stress could regulate the phosphorylation of Tyr 789 , a critical tyrosine residue for RPTPalpha signaling that modulates the function of Grb 2 and the activation of Src family kinases . ^^^ We further reported a novel finding that , besides the inhibition of RPTPalpha ' s activity , H ( 2 ) O ( 2 ) at low to moderate concentrations ( 50 250 microM ) markedly suppressed the phosphorylation of RPTPalpha Tyr 789 and the association of RPTPalpha with Grb 2 in cultured cells , which may result from inhibition of such a PP 1 sensitive but Src / Yes / Fyn independent tyrosine kinase . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
The NH ( 2 ) terminal proline rich collagen homology 2 ( CH 2 ) domain of p66shc associates with full length grb 2 in vitro via the COOH terminal src homology 3 ( C SH 3 ) domain of grb 2 . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
The epithelial podosome like structures specifically contain Arp2 / 3 complex , cortactin , dynamin , gelsolin , N WASP , VASP , Grb 2 and src like kinase ( s ) . ^^^ The maintenance of the F actin core and the surrounding hemidesmosomes depends on actin polymerization , src family kinases and Grb 2 , but not on microtubular integrity . ^^^
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P62993 and P12931	Pubmed	SVM Score :0.0
NA