| Interacting proteins: P28340 and P12931 |
Pubmed |
SVM Score :0.91084215 |
| A peptide derived from p34cdc2 , cdc 2 ( 6 20 ) NH 2 with the amino acid sequence of KVEKIGEGTYGVVYK amide , was found to be a specific and efficient substrate for a pp60c src related protein tyrosine kinase from bovine spleen ( STK ) . 0.91084215^^^ |
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| Interacting proteins: P28340 and P12931 |
Pubmed |
SVM Score :0.0 |
| We describe recent work that bears upon these unresolved problems : ( 1 ) p60c src is phosphorylated during mitosis on threonine and serine residues by the protein kinase encoded by the mammalian homologue of cdc 2 , suggesting that c src may contribute to the phenotype of mitotic cells ; ( 2 ) multiple regions in the amino terminal portion of p60src are required for its proper intracellular localization a short signal for myristylation and signals for association with cytoplasmic granules and with perinuclear and plasma membranes ; and ( 3 ) regions ( called SH 3 and SH 2 ) upstream of the kinase domain modulate the behavior of p60src in complex ways , with some mutations in SH 2 rendering p 60 host dependent for transformation . ^^^ |
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| Interacting proteins: P28340 and P12931 |
Pubmed |
SVM Score :0.0 |
| Both CDC 2 and CDC 28 contain the consensus sequences for the ATP binding and phosphorylation acceptor sites of protein kinases such as bovine cAMP dependent protein kinase ( bov PK ) and the src family of viral oncogene products . . ^^^ |
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| Interacting proteins: P28340 and P12931 |
Pubmed |
SVM Score :0.0 |
| Additional mutants lacking one ( TTAc src ) , two ( AASc src ) and three ( AAAc src ) cdc 2 phosphorylation sites had slightly higher kinase activity than wild type pp60c src in interphase cells and were not activated during mitosis . ^^^ |
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| Interacting proteins: P28340 and P12931 |
Pubmed |
SVM Score :0.0 |
| A study with src and cdc 2 derived phosphopeptides . ^^^ |
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| Interacting proteins: P28340 and P12931 |
Pubmed |
SVM Score :0.0 |
| Cdc 2 mediated modulation of pp60c src activity . ^^^ Phosphorylation by Cdc 2 partially reactivated Csk inactivated pp60c src . ^^^ Moreover , phosphorylation of Csk treated pp60c src by Cdc 2 also facilitated complete reactivation by the protein tyrosine phosphatase CD 45 or by a synthetic phosphopeptide corresponding to the C terminal , regulatory phosphorylation site ( Tyr 527 ) . ^^^ These data indicate that the Src homology 2 domain of Csk phosphorylated pp60c src was more accessible for intermolecular interactions and that Tyr 527 was more readily dephosphorylated after treatment with Cdc 2 . ^^^ In conjunction with in vivo studies , these data suggest that Cdc 2 is involved in the regulation of pp60c src during mitosis . . ^^^ |
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| Interacting proteins: P28340 and P12931 |
Pubmed |
SVM Score :0.0 |
| The interaction was mediated through the Src homology 3 domain of Lck and was selective , as only the active form of Cdc 2 was found to associate with Lck . ^^^ An association between active Cdc 2 and the Src related kinases Lyn and Fyn was also demonstrated , although Cdc 2 was not found associated with the tyrosine kinases , Csk and Syk . ^^^ |
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| Interacting proteins: P28340 and P12931 |
Pubmed |
SVM Score :0.0 |
| PtdIns ( 4 , 5 ) P 2 stimulated the phosphorylation of GST Shc without any effect on the c Src activity as judged by both its autophosphorylation and phosphorylation of exogenous substrate , Cdc 2 peptide . ^^^ |
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| Interacting proteins: P28340 and P12931 |
Pubmed |
SVM Score :0.0 |
| Phosphorylation of the Src substrate Sam 68 by Cdc 2 during mitosis . ^^^ |
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| Interacting proteins: P28340 and P12931 |
Pubmed |
SVM Score :0.0 |
| The relative efficiencies of the catalytic domain of the src family kinase pp60c src in phosphorylating four peptide substrates including ( 1 ) src optimal peptide ( AEEEIYGEFEAKKKK ) , ( 2 ) `` YEEI peptide ' ' ( KKTHQEEEEPQYEEIPIYL ) , ( 3 ) cdc 2 ( 6 20 ) ( KVEKIGEGTYGVVYK ) , ( 4 ) src autophosphorylation site peptide ( ADFGLARLIEDNEYTARG ) and the relative efficiencies of its SH 2 domain in binding the phosphorylated forms of these peptide substrates were compared . ^^^ The results show that the src optimal peptide , `` YEEI peptide , ' ' cdc 2 ( 6 20 ) peptide were phosphorylated by the catalytic domain with high efficiency and that the phosphorylated form of all three peptides could bind the SH 2 domain of the kinase , confirming the hypothesis proposed by Songyang and co workers that the catalytic domain of pp60c src phosphorylates sites which are recognized by its own SH 2 domain ( Songyang et al . ( 1995 ) Nature 373 , 536 539 ) . ^^^ |
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| Interacting proteins: P28340 and P12931 |
Pubmed |
SVM Score :0.0 |
| The compound was selective toward other protein kinases : the Src IC 50 value was lower than those for Cdc 2 ( > 500 fold ) , epidermal growth factor ( EGF ) receptor ( 7 . 5 fold ) , and vascular endothelial growth factor receptor ( > 50 fold ) , and for 5 Abl ( 15 fold ) and focal adhesion kinase ( Fak ) ( > 25 fold ) . ^^^ |
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| Interacting proteins: P28340 and P12931 |
Pubmed |
SVM Score :0.0 |
| According to ( 1 ) its immunologic cross reactivity with the monoclonal anti cSrc antibody , m 327 , and with the N terminally directed monoclonal cSrc 2 17 antibody , ( 2 ) its inhibition by the C terminal cSrc kinase , CSK , and ( 3 ) its specificity for phosphorylating tyrosine 15 in the cdc 2 ( 6 20 ) peptide kinase substrate , we conclude that the kinase we have isolated represents porcine cSrc . ^^^ |
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| Interacting proteins: P28340 and P12931 |
Pubmed |
SVM Score :0.0 |
| Sequence analysis revealed that golgin 67 is a highly coiled coil protein , with potential Cdc 2 and Src kinase phosphorylation motifs . ^^^ |
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| Interacting proteins: P28340 and P12931 |
Pubmed |
SVM Score :0.0 |
| Recent reports utilizing new methodologies combined with the latest genome information have shown that activation of several kinases including protein kinase A , protein kinase C , p 34 ( cdc 2 ) / cyclin B kinase , casein kinase 1 , mitogen activated protein ( MAP ) kinase and pp 60 ( src ) kinase can lead to phosphorylation at 12 of the 21 serine and two of the six tyrosine residues in the C terminal region of connexin 43 . ^^^ |
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| Interacting proteins: P28340 and P12931 |
Pubmed |
SVM Score :0.0 |
| We demonstrate the effectiveness of this approach by mutational analysis of peptide substrates derived from human cyclin dependent kinase , Cdc 2 , which contains a phosphorylation site of human c Src protein tyrosine kinase . . ^^^ |
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| Interacting proteins: P28340 and P12931 |
Pubmed |
SVM Score :0.0 |
| Recent technologies and investigations of interacting proteins have shown that activation of several kinases including protein kinase A , protein kinase C ( PKC ) , p 34 ( cdc 2 ) / cyclin B kinase , casein kinase 1 ( CK 1 ) , mitogen activated protein kinase ( MAPK ) and pp 60 ( src ) kinase can lead to phosphorylation of the majority of the 21 serine and two of the tyrosine residues in the C terminal region of Cx 43 . ^^^ |
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| Interacting proteins: P28340 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P28340 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P28340 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P28340 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P28340 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P28340 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P28340 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P28340 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P28340 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P28340 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P28340 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P28340 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
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