| Interacting proteins: P12931 and P18206 |
Pubmed |
SVM Score :0.0 |
| The 5 src and activated c src kinases were targeted to adhesion plaques by fusion to the talin binding sequence of vinculin . ^^^ |
|
| Interacting proteins: P12931 and P18206 |
Pubmed |
SVM Score :0.0 |
| Paxillin binds in vitro to the focal adhesion protein vinculin as well as to the SH 3 domain of c src and , when tyrosine phosphorylated , to the SH 2 domain of 5 crk . ^^^ |
|
| Interacting proteins: P12931 and P18206 |
Pubmed |
SVM Score :0.0 |
| The phosphorylation of vinculin on tyrosine was reconstituted in vanadate treated COS 7 cells coexpressing c Src . ^^^ Tyrosine residue 1065 located in the vinculin tail domain was phosphorylated by c Src in vitro . ^^^ |
|
| Interacting proteins: P12931 and P18206 |
Pubmed |
SVM Score :0.0 |
| First , the loss of germ cells from the seminiferous epithelium during androgen suppression was associated with a surge in myotubularin related protein 2 ( MTMR 2 , a lipid phosphatase , in which adult MTMR 2 / mice were recently shown to be azoospermic because of the loss of cell adhesion function between germ and Sertoli cells ) ; kinases : phosphatidylinositol 3 kinase , c Src , and C terminal Src kinase ; adaptors : alpha actinin , vinculin , afadin , and p 130 Crk associated protein ; and AJ integral membrane proteins at the ectoplasmic specialization ( ES , a testis specific cell cell actin based AJ type ) site : N cadherin , beta catenin , integrin beta 1 , and nectin 3 . ^^^ |
|
| Interacting proteins: P12931 and P18206 |
Pubmed |
SVM Score :0.0 |
| After thrombin induced platelet aggregation mediated by fibrinogen binding to GP IIb IIIa on adjacent platelets , we detected a redistribution of spectrin , talin , vinculin , pp60c src , and pp62c yes to the fraction that sediments at 15 , 600 10 g . ^^^ These results are consistent with the possibilities that 1 ) GP IIb IIIa , pp60c src , pp62c yes , and GAP associate with a membrane skeleton fraction that contains spectrin , vinculin , and talin , 2 ) the association of GP IIb IIIa with adhesive ligand in a platelet aggregate causes components of the membrane skeleton to undergo altered association with cytoplasmic actin filaments , and 3 ) many of the proteins phosphorylated on tyrosine residues in activated platelets are components of the cytoskeleton . ^^^ |
|
| Interacting proteins: P12931 and P18206 |
Pubmed |
SVM Score :0.0 |
| In order to determine the role of pp 60 ( c src ) and related kinases in these processes , we have compared the dynamic reorganization of phosphotyrosine , vinculin , focal adhesion kinase and tensin in cells with altered expression of Src family kinases . ^^^ |
|
| Interacting proteins: P12931 and P18206 |
Pubmed |
SVM Score :0.0 |
| Here we show that gelsolin coprecipitates some of the focal adhesion associated proteins such as c Src , phosphoinositide 3 kinase ( PI3K ) , p 130 ( Cas ) , focal adhesion kinase , integrin alpha ( 5 ) beta ( 3 ) , vinculin , talin , and paxillin . ^^^ |
|
| Interacting proteins: P12931 and P18206 |
Pubmed |
SVM Score :0.0 |
| ATP depletion increased the content of Tyr ( 416 ) Src , the activated form of this kinase . c Src activation was associated with an increase in the tyrosine phosphorylation state of beta catenin , paxillin , and vinculin , three c Src substrate proteins that localize to and regulate cell contact sites . ^^^ |
|
| Interacting proteins: P12931 and P18206 |
Pubmed |
SVM Score :0.0 |
| Furthermore , p FAK Tyr ( 397 ) was shown to coimmunoprecipitate with beta 1 integrin , vinculin , and c Src both in vitro and in vivo using Sertoli germ cell cocultures and seminiferous tubules , respectively . ^^^ |
|
| Interacting proteins: P12931 and P18206 |
Pubmed |
SVM Score :0.0 |
| The addition of 0 . 2 unit / mL thrombin caused aggregation dependent redistribution of cytoskeletal proteins ( actin binding protein , talin , vinculin , and alpha actinin ) , glycoproteins ( GPIIb IIIa , PECAM ) , and signaling molecules ( PI 3 kinase , pp60c src ) to the cytoskeletal fraction of platelets . ^^^ Aggregation ( 60 80 % of control ) was restored if 1 unit / mL thrombin was added , but there was no corresponding redistribution of actin binding protein , talin , vinculin , alpha actinin , GPIIb IIIa , PECAM , PI 3 kinase , and pp60c src to the cytoskeleton . ^^^ |
|
| Interacting proteins: P12931 and P18206 |
Pubmed |
SVM Score :0.0 |
| Immunocytochemistry , sucrose density gradient sedimentation , co immunoprecipitation analyses and in vitro binding assays have shown that polycystin 1 associates with the focal adhesion proteins talin , vinculin , p130Cas , FAK , alpha actinin , paxillin and pp60c src in subconfluent normal human fetal collecting tubule ( HFCT ) epithelia when cell matrix interactions predominate . ^^^ |
|
| Interacting proteins: P12931 and P18206 |
Pubmed |
SVM Score :0.0 |
| With fluorescence and interference reflection microscopy ( IRM ) , we compared the regional distribution of calspectin , its interacting proteins ( nonerythroid protein 4 . 1 and calpactin ) , alpha actinin , and vinculin in NRK cells and their avian sarcoma virus ( ASV ) or temperature sensitive ( ts ) Rous sarcoma virus ( RSV ) transformed cells . ^^^ |
|
| Interacting proteins: P12931 and P18206 |
Pubmed |
SVM Score :0.0 |
| With the exception of calpactin 1 , the major tyrosine phosphoproteins detected in immunoblots appeared to be different from several previously characterized substrates of pp60v src with similar molecular masses ( ezrin , vinculin , and the fibronectin receptor ) . . ^^^ |
|
| Interacting proteins: P12931 and P18206 |
Pubmed |
SVM Score :0.0 |
| The use of Rous sarcoma virus transformation mutants with differing tyrosine kinase activities to study the relationships between vinculin phosphorylation , pp60v src location and adhesion plaque integrity . ^^^ One of the putative substrates for the src gene product ( pp60v src ) of RSV is the cytoskeletal protein vinculin , giving rise to the hypothesis that tyrosine specific phosphorylation of vinculin disrupts adhesion plaque integrity , leading to the characteristic rounded morphology of RSV transformed cells . ^^^ We have investigated this hypothesis by analysing the properties of fibroblasts transformed by conditional and non conditional mutants of RSV which confer different morphologies on infected cells , with respect to formation of microfilament bundles , formation of vinculin containing adhesion plaques , the deposition of a fibronectin containing extracellular matrix , the localization of pp60v src and the tyrosine specific phosphorylation of vinculin . ^^^ However , these cells had a fully active pp60v src kinase , had pp60v src concentrated in their adhesion plaques and contained vinculin which was heavily phosphorylated on tyrosine residues . ^^^ Cells transformed by a recovered avian sarcoma virus , rASV 2234 . 3 exhibited a markedly fusiform morphology with pp60v src concentrated in well developed adhesion plaques and an elevation of the phosphotyrosine content of vinculin . ^^^ |
|
| Interacting proteins: P12931 and P18206 |
Pubmed |
SVM Score :0.0 |
| The absence of pp60src from the adhesion plaques was not due to lack of myristylation of the src protein , and tyrosine phosphorylation of vinculin was not related to fibronectin expression . ^^^ |
|
| Interacting proteins: P12931 and P18206 |
Pubmed |
SVM Score :0.0 |
| Irrespective of cell morphology , the extent of tyrosine phosphorylation of vinculin was high in all cells transformed by viruses carrying the src gene , but low in those transformed by viruses expressing the fps gene . ^^^ |
|
| Interacting proteins: P12931 and P18206 |
Pubmed |
SVM Score :0.0 |
| When compared with tryptic maps of vinculin phosphorylated by src kinase , no overlapping phosphorylated peptides were found . ^^^ |
|
| Interacting proteins: P12931 and P18206 |
Pubmed |
SVM Score :0.0 |
| Vinculin phosphorylation by the src kinase : inhibition by chlorpromazine , imipramine and local anesthetics . ^^^ |
|
| Interacting proteins: P12931 and P18206 |
Pubmed |
SVM Score :0.0 |
| Vinculin phosphorylation by the src kinase . ^^^ The phospholipid binding specificity correlated with the ability of these phospholipids to enhance vinculin phosphorylation by the src kinase . ^^^ |
|
| Interacting proteins: P12931 and P18206 |
Pubmed |
SVM Score :0.0 |
| Paxillin has been shown to have binding sites for the SH 3 domain of Src and the SH 2 domain of Crk in vitro and to coprecipitate with two other focal adhesion proteins , vinculin and focal adhesion kinase ( p125fak ) . ^^^ |
|
| Interacting proteins: P12931 and P18206 |
Pubmed |
SVM Score :0.0 |
| Immunoprecipitation experiments with specific antibodies against potential candidate proteins in the molecular mass range around 130kDa revealed positive results for the focal adhesion kinase FAK and the p 130 Src substrate while negative results were obtained for the GTPase activating protein GAP , the phospholipase C gamma 1 , the Janus kinase JAK 1 and vinculin . ^^^ |
|
| Interacting proteins: P12931 and P18206 |
Pubmed |
SVM Score :0.0 |
| Paxillin is a 68 kD focal adhesion phosphoprotein that interacts with several proteins including members of the src family of tyrosine kinases , the transforming protein 5 crk , and the cytoskeletal proteins vinculin and the tyrosine kinase , focal adhesion kinase ( FAK ) . ^^^ |
|
| Interacting proteins: P12931 and P18206 |
Pubmed |
SVM Score :0.0 |
| It is a multi domain adapter protein capable of interacting with several structural and signaling proteins including vinculin , FAK , PYK 2 , Src and Crk . ^^^ |
|
| Interacting proteins: P12931 and P18206 |
Pubmed |
SVM Score :0.0 |
| The expression of other Src substrates and interacting proteins , such as p 120 Cas , p 130 Cas , vinculin , Fak kinase , and the p 85 phosphatidylinositol 3 kinase subunit either did not change or slightly increased during PMA treatment . ^^^ |
|
| Interacting proteins: P12931 and P18206 |
Pubmed |
SVM Score :0.0 |
| What do Src kinase , Ras guanine nucleotide exchange factor , cytidylyltransferase , protein kinase C , phospholipase C , vinculin , and DnaA protein have in common . ^^^ |
|
| Interacting proteins: P12931 and P18206 |
Pubmed |
SVM Score :0.0 |
| Treatment of an intestinal epithelial cell line with LTD ( 4 ) induced rapid tyrosine phosphorylation of vinculin , which was blocked by the Src family tyrosine kinase inhibitor PP 1 . ^^^ |
|
| Interacting proteins: P12931 and P18206 |
Pubmed |
SVM Score :0.0 |
| Integrin engagement caused the formation of phosphorylated pp 60 ( src ) / focal adhesion kinase complexes and the aggregation of focal adhesion plaques containing the beta ( 3 ) integrin subunit , the cytoskeletal proteins vinculin , cortactin , and paxillin , the tyrosine kinases focal adhesion kinase and pp 60 ( src ) , the adapter protein Grb 2 , and the mitogen activated protein kinase ERK 2 . ^^^ |
|
| Interacting proteins: P12931 and P18206 |
Pubmed |
SVM Score :0.0 |
| Here , endothelial GM 7373 cells spread and organize focal adhesion plaques on immobilized FGF 2 , fibronectin ( FN ) , and vitronectin ( VN ) . alpha ( 5 ) beta ( 3 ) integrin , paxillin , focal adhesion kinase , vinculin and pp 60 ( src ) localize in cell substratum contact sites on FGF 2 , FN or VN . ^^^ |
|
| Interacting proteins: P12931 and P18206 |
Pubmed |
SVM Score :0.0 |
| We show that , in KM12C colon cancer cells , elevated Src activity causes the components of adherens junctions , including vinculin , to be redistributed to Src induced integrin adhesion complexes . ^^^ |
|
| Interacting proteins: P12931 and P18206 |
Pubmed |
SVM Score :0.0 |
| Dramatic alterations in the distribution of vinculin and in the expression and phosphorylation state of both FAK and Src kinases were also detected . ^^^ |
|
| Interacting proteins: P12931 and P18206 |
Pubmed |
SVM Score :0.0 |
| This mechanism was characterized by decreased recruitment of focal adhesion kinase ( FAK ) and vinculin to the alpha ( 5 ) beta 3 integrin and reduced FAK and Src activation in response to VEGF . ^^^ |
|
| Interacting proteins: P12931 and P18206 |
Pubmed |
SVM Score :0.0 |
| In addition , under anoikis stress , the induction of the Y397F mutant in 5 Src transformed FAK ( / ) cells selectively led to a decrease in the level of p 130 ( Cas ) , but not other focal adhesion proteins such as talin , vinculin , and paxillin . ^^^ |
|
| Interacting proteins: P12931 and P18206 |
Pubmed |
SVM Score :0.0 |
| Ang 2 induced AT1R activation via Gq / 11 stimulates phospholipases A 2 , C , and D , and activates inositol trisphosphate / Ca2+ signaling , protein kinase C isoforms , and MAPKs , as well as several tyrosine kinases ( Pyk 2 , Src , Tyk 2 , FAK ) , scaffold proteins ( G protein coupled receptor kinase interacting protein 1 , p130Cas , paxillin , vinculin ) , receptor tyrosine kinases , and the nuclear factor kappaB pathway . ^^^ |
|
| Interacting proteins: P12931 and P18206 |
Pubmed |
SVM Score :0.0 |
| The puncta co localized with focal adhesion kinase ( FAK ) and Src , and defined the distal tips of cell matrix adhesion sites identified with paxillin and vinculin . ^^^ |
|
| Interacting proteins: P12931 and P18206 |
Pubmed |
SVM Score :0.0 |
| Although the expression and / or activation status of focal adhesion complex components such as Src , FAK , and vinculin were not affected by Rac 1 deletion , the number and size of adhesion plaques were significantly reduced , and the molecular complex between Src , FAK , and vinculin was dissembled in Rac 1 null cells . ^^^ |
|
| Interacting proteins: P12931 and P18206 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P12931 and P18206 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P12931 and P18206 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P12931 and P18206 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P12931 and P18206 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P12931 and P18206 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P12931 and P18206 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P12931 and P18206 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P12931 and P18206 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P12931 and P18206 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P12931 and P18206 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P12931 and P18206 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P12931 and P18206 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P12931 and P18206 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P12931 and P18206 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P12931 and P18206 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P12931 and P18206 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P12931 and P18206 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P12931 and P18206 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P12931 and P18206 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P12931 and P18206 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P12931 and P18206 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P12931 and P18206 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P12931 and P18206 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P12931 and P18206 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P12931 and P18206 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P12931 and P18206 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P12931 and P18206 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P12931 and P18206 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P12931 and P18206 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P12931 and P18206 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P12931 and P18206 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P12931 and P18206 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P12931 and P18206 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P12931 and P18206 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P12931 and P18206 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P12931 and P18206 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P12931 and P18206 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P12931 and P18206 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P12931 and P18206 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P12931 and P18206 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P12931 and P18206 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P12931 and P18206 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P12931 and P18206 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P12931 and P18206 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P12931 and P18206 |
Pubmed |
SVM Score :0.0 |
| NA |
|