Pubmed abstracts for Protein-Protein Interaction search result :

Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
It is clear , however , that c src is the prototype of a family of at least 8 closely related genes encoding protein tyrosine kinases , the other family members being blk , c fgr , fyn , hck , lck , lyn , and c yes . ^^^
Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
Human HCK , C SRC ( encoding p 60 Src PTK ) , C FGR and LCK ( encoding p 56 Lck , PTK ) genes thus share very similar exon / intron structures for the conserved exons . ^^^ To date , the complete genomic structure of human src family members is only available for the C FGR gene ( encoding p 55 Fgr , PTK ) . ^^^
Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
The c fgr proto oncogene , which is a member of the c src gene family , encodes the cytoplasmic tyrosine kinase p55c fgr . ^^^
Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
Peptide mapping demonstrated that the fyn protein was distinct from the closely related c src and c fgr proteins . ^^^
Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
To date the src gene family consists of at least 9 closely related protein tyrosine kinases belonging to the non receptor type of kinases : c src , c yes , c fgr , fyn , lyn , lck , hck , tkl and bkl . ^^^
Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
A phosphorylated peptide corresponding to the carboxyl terminus of the c fgr protein also bound to pp60c src ( F 527 ) , but with weaker affinity . ^^^
Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
The c fgr proto oncogene specifies a nonreceptor protein tyrosine kinase , p55c fgr , a member of the src family . ^^^
Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
Two members of the src proto oncogene family of intracellular tyrosine kinases , c fgr and hck , are selectively expressed in differentiated myeloid cells . ^^^
Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
The mutant c fgr protein ( p58c fgr / F523 ) containing Phe 523 instead of Tyr 523 exhibited transforming activity in NIH 3T3 cells like other protein tyrosine kinases of the src family , but normal p58c fgr ( p58c fgr / wt ) did not . ^^^ Surprisingly , transfection of the normal c fgr gene into NIH 3T3 cells resulted in induction of sodium fluoride ( NaF ) sensitive alpha naphthyl butyrate esterase ( alpha NBE ) , a marker enzyme of cells of monocytic origin , which was not induced in 5 src , 5 fgr , or lyn transfected NIH 3T3 cells . ^^^
Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
We examined the expression of four different src family genes ( lck , c src , fyn , and c fgr ) in addition to the expression of IL 2 , c fos , c myc , and actin genes in murine T cells which were activated with PMA plus ionomycin or PMA plus anti CD 3 mAb . ^^^
Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
Computer search showed the presence in the putative third intracellular loop of a motif resembling that described in the non receptor type protein tyrosine kinases ( c src , c yes , c fgr , etc . . . ) . ^^^
Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
When L 6 alpha 1 cells from myotubes , the levels of c abl , c myb , and c Ha ras transcripts remain unchanged , the level of c N ras RNA is augmented , the level of c erbB RNA is markedly reduced , and all other c onc transcripts ( c erbA , c sis , c src , c fes , c fms , c fos , c myc , c Ki ras , and the putative tyrosine kinase transcript of the c fgr gene ) become hardly , if at all , detectable . ^^^
Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
Differential expression and regulation of the c src and c fgr protooncogenes in myelomonocytic cells . ^^^ While c src transcripts were detected in myeloid leukemia cells representative of all stages of differentiation , the highly related gene c fgr was expressed at high levels only at later developmental stages , both in normal cells committed to the monocytic lineage and in leukemic cells with a differentiated myelomonocytic phenotype . ^^^ When bone marrow derived monocytic cells were synchronized and stimulated to proliferate with CSF 1 , c fgr transcripts ( but not transcripts from the highly related genes c src or c yes ) were induced 8 hr after the addition of CSF 1 and decreased to low levels by 20 hr as the monocytic cells entered S phase . ^^^
Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
This interaction also represents a possible precedent by which other members of the src family ( c src , c yes , c fgr , etc . ) may be found to interact with mammalian growth receptors . . ^^^
Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
Splicing sites for exons 4 to 12 , which encode both conserved phospholipase C like and catalytic domains of the Src like PTKs , arise exactly at the same position for the human lck , human c src and c fgr genes . ^^^
Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
Normal murine bone marrow derived monocytic cells were found to contain transcripts for c fgr and hck , two members of the src family of proto oncogene tyrosine kinases . ^^^ However , when compared to sequences previously reported for human c fgr derived from EBV transformed B cells and heterogeneous peripheral blood cells , the c fgr cDNA derived from normal murine monocytic cells differed significantly in sequence from amino acids 12 62 in the amino terminal domain of the protein which may mediate the substrate specificity and subcellular location of the src family of protein tyrosine kinases . . ^^^
Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
Interestingly , the splicing points of the c fgr gene were identical with those of the c src gene throughout the seven exons , suggesting that the two proto oncogenes were generated by gene duplication of an ancestral gene containing intervening sequences . ^^^
Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
Sequence similarity with human c src , c yes , and fyn translational products began at amino acid position 76 of the predicted c fgr protein and extended nearly to its C terminus . ^^^
Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
By screening both human genomic and cDNA libraries using 5 yes DNA as a probe , we obtained DNA clones of the c yes gene , the pseudogene of c yes , c fgr gene and c src gene and two novel yes related genes , fyn and lyn . ^^^
Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
A 2 . 8 kilobase transcript of the c syn gene , which differs in size from those of the c yes , c src , and c fgr genes , was observed in various cell types . ^^^
Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
No significant expression of c src , c ras , c fos , c fgr , c fps , c fes , c fms , c yes , c rel , c abl , c mos , c sis , and c erb B genes was found in the patients . c myc and c myb expression as having pathogenic and clinical significance is discussed . . ^^^
Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
Regulation of c Fgr protein kinase by c Src kinase ( CSK ) and by polycationic effectors . ^^^ The protein tyrosine kinase expressed by the protooncogene c fgr is phosphorylated and down regulated in vitro by the c Src kinase ( CSK ) . ^^^ CSK catalyzed phosphorylation affects Tyr 511 of c Fgr , homologous to Tyr 527 of c Src and it prevents the autophosphorylation normally occurring at c Fgr Tyr 400 , homologous to c Src Tyr 416 . ^^^ These data support the concept that down regulation of c Fgr by Tyr 511 phosphorylation is prevented if Tyr 400 is also phosphorylated and they are consistent with an outcompetition of phospho Tyr 511 from the Src homology 2 domain by phospho Tyr 400 , which , in c Fgr , is surrounded by an amino acid sequence divergent from that of the other Src related protein tyrosine kinases . . ^^^
Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
The c src gene family has nine known members ( blk , c fgr , fyn , hck , lck , lyn , c src , c yes and yrk ) , each encoding a cytoplasmic protein tyrosine kinase ( PTK ) believed to be involved in signal transduction . ^^^
Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
The c fgr proto oncogene is a member of the src family of intracellular protein tyrosine kinases . ^^^ In contrast to all other members of the src family , the presumed murine and human c fgr homologues have a low degree of homology in their amino terminal domains which likely mediate the association of c fgr proteins with signalling complexes and other targets ; murine and human c fgr proteins exhibit other functional differences as well . ^^^
Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
USA 90 , 3631 3635 ] . p 50 is an excellent substrate for TPK IIB , exhibiting very favourable kinetic constants ( Km = 0 . 07 microM , kcat = 1 . 5 ) and incorporating up to 4 mol P / mol protein . p 50 is , however , a weak substrate for the Src related protein kinases Lyn and c Fgr . ^^^
Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
We also show that the HS 1 peptide can be used for the specific monitoring of p72syk since neither the two Src related c Fgr and Lyn kinases ( needing a hydrophobic instead of acidic residue at position 1 ) nor CSK appreciably phosphorylate it . . ^^^
Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
The proto oncogene c fgr is a member of the c src gene family of cytoplasmic tyrosine kinases . ^^^
Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
The c fgr gene is a member of the src family of proto oncogene tyrosine kinases . ^^^
Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
The c fgr gene is a member of the Src family of protooncogene tyrosine kinases . ^^^
Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
We found no detectable transcripts of 9 proto oncogenes ( c sis , c met , c src , c lyn , c fgr , c ros , c pim , Blym and N myc ) , but detected transcripts of 12 other proto oncogenes ( int 2 , erbB , c abl , c raf 1 , c fyn , K ras , H ras , c mos , c myc , c myb , c fos , and c jun ) . ^^^
Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
The sequential phosphorylation of p50 / HS1 as well as its specific blockage by the HS 1 phosphopeptide is also observable if c Fgr is replaced by two other Src related kinases , namely , Lyn and Fyn , as secondary phosphorylating agents . ^^^
Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
The phosphotyrosyl phosphatase ( PTPase ) specificity of phosphotyrosyl phosphatase activator ( PTPA ) stimulated protein phosphatase ( PP ) 2A ( D ) ( rabbit muscle ) and a bona fide PTP 1B ( Xenopus laevis oocytes ) were examined in vitro using phosphotyrosine containing peptides , derived from the phosphorylation sites of p34cdc2 , p50 / HS1 protein , Abl , c Src and c Fgr , as well as the intact phosphoprotein p50 / HS1 and the Src related tyrosine kinases , Lyn and c Fgr . ^^^ Lyn and c Fgr phosphorylated by C terminal Src kinase ( CSK ) at their down regulatory site are resistant to the action of both PTPases while the [ Phe 6 ] Src ( 514 533 ) phosphopeptide , representing the highly similar site affected by CSK in c Src , is readily dephosphorylated by both PTPases , although to a different extent . ^^^
Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
The tyrosine kinases c Fgr , Fyn , C terminal Src kinase and p72syk , as well as the Ser / Thr specific cAMP dependent protein kinase and protein kinases CK 1 and CK 2 do not phosphorylate p 57 . ^^^
Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
Specific antibodies against c Src , Fyn , c Yes , c Fgr , Lck , Lyn , Hck , and Blk proteins did not recognize the p 57 Xenopus tyrosine kinase . ^^^
Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
The Wiskott Aldrich syndrome protein was identified as a protein that associated selectively with the SH 3 domains derived from c Src , p85alpha , phospholipase Cgamma 1 , and c Fgr . ^^^
Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
To identify proteins present in complexes with Src family kinases , we subjected p 58 ( c fgr ) or p53 / 56 ( lyn ) immunoprecipitates from Triton 10 100 lysates of PMN incubated on fibrinogen coated surfaces to in vitro kinase assays . ^^^ Assays on p 58 ( c fgr ) or p53 / 56 ( lyn ) immunoprecipitates from Triton lysates of PMN induced to spread over fibrinogen in response to TNF alpha showed that several proteins form complexes with Src family kinases and can be phosphorylated in vitro . ^^^ Formation of protein complexes containing Src family kinases and p 72 ( syk ) required PMN spreading because p 72 ( syk ) was not detected in p 58 ( c fgr ) or p53 / 56 ( lyn ) immunoprecipitates from PMN , which were stimulated with TNF alpha , in suspension . ^^^
Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
The dephosphorylated HS 1 ( 320 329 ) peptide is totally ineffective , while the stimulatory efficacy of other phosphopeptides derived from the polyoma virus middle T antigen ( 393 402 ) sequence , c Src , and c Fgr autophosphorylation sites , and the C terminal c Src site ( Tyr 527 ) is variable and correlates reasonably well with the predicted affinity for the c Fgr SH 2 domain . ^^^ Interestingly , neither Syk and C terminal Src kinase nor three other members of the Src family ( Lyn , Lck , and Fyn ) are susceptible to stimulation by phosphopeptide , as observed with c Fgr . ^^^
Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
An eicosapeptide encompassing the C terminal tail of c Src ( Tyr 527 ) which is conserved in most Src related protein kinases , is phosphorylated by C terminal Src kinase ( CSK ) and by the two Src related protein kinases c Fgr and Lyn , with similar kinetic constants . ^^^ Sequence specificity of C terminal Src kinase ( CSK ) a comparison with Src related kinases c Fgr and Lyn . ^^^
Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
The 5 fgr oncogene encodes a chimeric oncoprotein composed of feline sarcoma virus ( FeSV ) derived gag and cellular derived actin and c Fgr sequences . 5 Fgr is myristoylated and membrane bound , two criteria which must be met for src kinases to induce cellular transformation . ^^^
Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
We have now identified tyrosine 222 as the HS 1 residue phosphorylated by the Src family protein kinases c Fgr and Lyn , and we show that a truncated form of HS 1 ( HS 1 208 401 ) lacking the N terminal putative DNA binding region and the C terminal Src homology 3 ( SH 3 ) domain is still able to undergo all the steps of sequential phosphorylation as efficiently as full length HS 1 . ^^^
Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
In this study , we demonstrate that the phosphorylation activity of five tyrosine kinases of the src family from both human erythrocytes ( lyn , hck and c fgr ) and bovine synaptosomes ( lyn and fyn ) was stimulated by treatment with 30 250 microM peroxynitrite . ^^^
Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
Novel association of the src family kinases , hck and c fgr , with CCR 3 receptor stimulation : A possible mechanism for eotaxin induced human eosinophil chemotaxis . ^^^ Coimmunoprecipitation studies revealed that binding of eotaxin to CCR 3 greatly enhanced association of the Src family kinases , Hck and c Fgr , with CCR 3 after internalization of CCR 3 . ^^^
Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
Additionally , we found that the Src family kinase c Fgr tyrosine phosphorylates PP 1 alpha in vitro . ^^^
Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
In the present study , we show that the catalytic subunits of human CK 2 , but not the regulatory beta subunits , are readily phosphorylated by the Src family protein tyrosine kinases Lyn and c Fgr to a stoichiometry approaching 2 mol phosphotyrosine / mol CK2alpha with a concomitant 3 fold increase in catalytic activity . ^^^
Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
Nine src family members are known including c Src , c Yes , c Lck , c Fyn , c Hck , c Lyn , c Blk , c Fgr and c Yrk . ^^^
Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
Probes specific for the other locus , c src 2 ( human ) , did not hybridize to polyadenylated RNA prepared from a variety of human cell lines . ^^^ Partial nucleotide sequence determinations of the loci demonstrated that c src 1 ( human ) is highly related to chicken c src and that c src 2 ( human ) is slightly more divergent . ^^^ We conclude that c src 1 ( human ) is the analog of c src ( chicken ) and that the duplicated locus , c src 2 ( human ) , may also be expressed . . ^^^
Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
The activation of int 1 and int 2 genes did not clearly correlate with an increase in the expression of oncogenes examined ; H ras , K ras , N ras , myc , raf , fgr , fms , erB , mos , and src genes . . ^^^
Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
The fyn , fgr , and lyn genes were characteristically expressed concomitantly with phenotypic changes and expressions of nuclear proto oncogenes , whereas src , lck , hck , and yes genes were not . ^^^
Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
Transfected NIH / 3T3 cells , each overexpressing a normal or activated version of the fyn , fgr , or src translational product , were examined using antibody to phosphotyrosine as a probe . ^^^
Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
In cell lines transformed by src or fgr oncogene encoding tyrosine kinase , ST 638 also inhibited phosphorylation of calpactin 1 ( p 36 ) without affecting that of the oncogene products . ^^^
Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
The fgr protooncogene is a member of the src family of protein tyrosine kinases . ^^^
Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
Two regions of phospholipase C ( amino acid residues 555 598 and 668 705 ) exhibited significant amino acid similarities to the products of various tyrosine kinase related oncogenes ( yes , src , fgr , abl , fps , fes , and tck ) . ^^^
Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
A restriction fragment length polymorphism ( RFLP ) at the human FGR gene , a member of the src family of protooncogenes , has been identified and used to locate FGR on the genetic linkage map of human chromosome 1p . ^^^
Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
Sis and N ras were moderately hyperexpressed , raf , Blym , and erbA were slightly hyperexpressed , and abl , erbB , fes / fps , fgr , fos , mos , myb , myc , N myc , rasHarvey , rasKirsten , ros , src , and yes were not hyperexpressed in the tumor compared to the control tissue . ^^^
Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
The JURKAT cDNA ( designated ptk JURKAT ) was homologous to but distinct from the src , yes and fgr oncogenes , which encode protein tyrosine kinases ( ATP : protein phosphotransferase , EC 2 . 7 . 1 . 37 ) . ^^^
Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
The anti yes serum did not recognize viral src , fps , or fgr proteins . ^^^
Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
Partial proteolytic digestion experiments indicate that this protein is identical to the 90 kd phosphoprotein found in a highly stable complex with the protein products of at least three members of the tyrosine kinase family of oncogenes ( src , fes , fgr ) . . ^^^
Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
Two dimensional tryptic peptide mapping revealed that p 56 is distinct from the proteins encoded by the cellular genes which are the progenitors of retroviral tyrosine protein kinases , src , yes , fgr , abl , fes , and ros . ^^^
Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
Specificity of protein interactions with highly related SRC homology ( SH ) domains of FGR and FYN protein tyrosine kinases . ^^^
Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
To examine the role of Src related proteins in human colon carcinoma we measured the tyrosine kinase activity of pp60c src ( Src ) , p62c yes ( Yes ) , p56lck ( Lck ) , p59fyn ( Fyn ) , p59hck ( Hck ) , p56lyn ( Lyn ) and p55c fgr ( Fgr ) from colonic cells . ^^^
Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
In this report , we have expressed normal and enzymatically activated versions of the fyn , fgr , and src translational products by transfection of appropriate cDNAs into mouse fibroblasts . ^^^
Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
Here we show that direct ligation of Fc gamma RII leads to activation and Triton 10 100 insolubility of the Src family kinase Fgr , without effect on the related myeloid Src family member Hck . ^^^
Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
Following synthesis in the cytoplasm , the transforming proteins encoded by the retroviral oncogenes src , yes , fps , fes , and fgr form complexes with hsp 90 and the hsp 90 cohort p 50 . ^^^
Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
Functional overlap in the src gene family : inactivation of hck and fgr impairs natural immunity . ^^^ We have generated mice with targeted disruptions of the src like genes hck and fgr to assess the role of these kinases in myeloid cell development and function . ^^^ Macrophages cultured from doubly homozygous , hck l ( ) fgr l animals retain many other normal functional properties , suggesting that the deficiency of these kinases is complemented by other src family members . ^^^
Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
However , three of these kinases ( src , yes and fgr ) have been initially identified as products of viral oncogenes and a fourth ( lck ) is activated and overexpressed in certain lymphomas . ^^^
Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
We have deleted the SH 2 and SH 3 domains from Fyn and Fgr and have generated two point mutations in residues completely conserved in all members of the Src family . ^^^
Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
Accompanying the RA induced differentiation , activities of src family PTKs Lyn and Fgr became detected and reached a plateau 2 days after the stimulation . ^^^
Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
Expression of the Src family kinases Src , Hck , and Fgr increases dramatically during myeloid cell development . ^^^ Src deficient mice exhibit functional abnormalities in only one myeloid cell type , the osteoclast , resulting in impaired bone remodeling and osteopetrosis , while hck / or fgr / mice have few and subtle myeloid cell deficiencies . ^^^ To determine whether these limited phenotypes are due to the coexpression of multiple Src family kinases with overlapping functions , we have intercrossed src / mice to hck / and fgr / mutants to produce double mutants . ^^^ In contrast , fgr / src / double mutants have no defects beyond those observed in src / animals . ^^^ Cultured normal murine osteoclasts express abundant amounts of Src , Hck , and Fgr and Hck levels are increased in src / osteoclasts . ^^^
Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
Deficiency of Src family kinases p59 / 61hck and p58c fgr results in defective adhesion dependent neutrophil functions . ^^^ Inflammation . 45 : 217 311 . ) To directly test whether these functional responses are dependent on the Src family kinases p59 / 61hck and p58c fgr , we examined adhesion dependent respiratory burst in PMNs isolated from hck / , fgr / , and hck / fgr / knockout mice . ^^^
Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
CHK / HYL did not affect the activity of Src , Hck , and Fgr in cultured bone marrow cells , although CSK negatively regulates Src family kinases . ^^^
Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
Deficiency of Src family kinases Fgr and Hck results in activation of erythrocyte K / Cl cotransport . ^^^ We found that mouse erythrocytes express the Src family kinases Fgr and Hck , as well as Lyn . ^^^ Abnormality of erythrocyte K / Cl cotransport in fgr / hck / double mutant animals represents the first demonstration that Src family kinases may be involved in regulation of membrane transport . . ^^^
Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
Csk overexpression resulted primarily in a down regulation of Fgr activity , an src PTK expressed in J774A . 1 , and hyperphosphorylation of several cellular proteins of 35 , 57 , 66 , 97 and 120 130 kDa . ^^^
Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
The Src family kinases , Fgr , Fyn , Lck , and Lyn , colocalize with coated membranes in platelets . ^^^ Src and Src family members are reported to be membrane associated , but detergent extraction studies have demonstrated a major difference in the solubility of Src compared with other members of the Src family ( Fgr , Fyn , Lck , Lyn , and Yes ) , suggesting that their subcellular distributions may be different . ^^^
Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
Identification of the Src family kinases , Lck and Fgr in platelets . ^^^ We have identified the Src family members , Lck and Fgr in resting human and rodent platelets and compared their subcellular distributions and tyrosine phosphorylation status to those of the other Src family kinases to gain insights into the signal transduction pathways active in maintaining platelets in the circulation . ^^^ We also compared the tyrosine phosphorylation status of Lck and Fgr to other Src family members in resting platelets using immunoprecipitation and immunoblotting . ^^^ All of these Src family members except Fgr exhibited substantial phosphotyrosine antibody labeling . ^^^
Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
By using co immunoprecipitation techniques and in vitro kinase assays , the uPAR associated proteins were identified as Janus ( Jak ) and Src non receptor protein tyrosine kinases ( PTK ) Jak 1 , Tyk 2 , and p 59 ( fyn ) , p53 / 56 ( lyn ) , p53 / 59 ( hck ) , and p 55 ( fgr ) . ^^^
Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
This interaction is not mediated by the src kinases Lyn and Fgr , since neither kinase coprecipitated with Syk . ^^^
Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
Using coprecipitation methods with convulxin coupled beads and antibodies against FcRgamma and the Src family , we showed that Fyn and Lyn , but not Yes , Src , Fgr , Hck , and Lck , were physically associated with the GPVI FcRgamma complex irrespective of stimulation . ^^^
Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
The Src family protein tyrosine kinase Fgr ( p 58 ) also displayed enhanced tyrosine phosphorylation after Fc gamma RI and Fc gamma RII crosslinking . ^^^
Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
Adhesion dependent degranulation of neutrophils requires the Src family kinases Fgr and Hck . ^^^ Analysis of lactoferrin secretion in hck / fgr / or PP 1 treated , suspended PMN showed that Src kinases are not implicated in degranulation dependent on activation of protein kinase C or increase in intracellular free Ca2+ but may play a role in the response to FMLP of cytochalasin B treated PMN . ^^^ These findings identify a role for Src family kinases in a signaling pathway leading to granule plasma membrane fusion and suggest that Fgr and Hck would be targets for pharmacological control of adhesion dependent degranulation in the inflammatory site . . ^^^
Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
Here , we report that urokinase treated differentiating U 937 monocyte like cells exhibit a rapid and transient inhibition of p56 / 59 ( hck ) and p 55 ( fgr ) whereas no changes in the activity of other Src family kinases , such as p53 / 56 ( lyn ) and p 59 ( fyn ) were observed . ^^^
Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
The activity of the other 5 members of the Src tyrosine kinases expressed in the rabbit heart ( Fyn , Fgr , Yes , Lyn , and Blk ) was not affected by ischemic PC . ^^^
Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
Several lines of evidence suggest that members of the src family of protein tyrosine kinases play important roles in macrophage activation by gram negative bacterial lipopolysaccharide ( LPS ) or the cytokine interferon gamma ( IFN gamma ) , but targeted disruption of three members of the src family ( hck , fgr , and lyn ) in mice failed to demonstrate a requirement for these particular kinases in macrophage activation . ^^^
Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
Negative regulation of phagocytosis in murine macrophages by the Src kinase family member , Fgr . ^^^ To pursue negative regulation of phagocytosis , we assessed the effect of the Src kinase family member , Fgr , on opsonin dependent phagocytosis by mouse macrophages . ^^^ After Fcgamma receptor cross linking , Fgr becomes associated with the immunoreceptor tyrosine based inhibition motif ( ITIM ) containing receptor , SIRPalpha ( a member of the signal regulatory protein family , also known as Src homology 2 domain containing protein tyrosine phosphatase [ SHP ] substrate 1 [ SHPS 1 ] , brain immunoglobulin like molecule with tyrosine based activation motifs [ BIT ] , and P 84 ) and potentiates the association of the phosphatase SHP 1 with SIRPalpha . ^^^
Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
Fcgamma receptor mediated phagocytosis in macrophages lacking the Src family tyrosine kinases Hck , Fgr , and Lyn . ^^^ We examined FcgammaR signaling and phagocytic function in bone marrow derived macrophages from mutant mice lacking the major Src family kinases expressed in these cells , Hck , Fgr , and Lyn . ^^^
Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
PP 1 , an inhibitor of Src family kinases , also attenuated the release of primary and secondary granules , and neutrophils from mice deficient in the Src family kinases Hck , Fgr , and Lyn were also defective in secondary granule release . ^^^
Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
Western blot analysis identified the src like protein tyrosine kinases p 53 ( lyn ) , p 56 ( lyn ) , p56 / 59 ( hck ) , p 55 ( fgr ) , and p 56 ( lck ) whereas p 60 ( src ) , p 62 ( yes ) , p 55 ( blk ) , and p 59 ( fyn ) were not detected . ^^^
Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
The members of the Src kinase family are expressed in a wide variety of tissues , but some of them such as Blk , Hck , Fgr , Lck and Lyn are found primarily in hematopoietic cells . ^^^
Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
Inhibition of beta 2 integrin receptor and Syk kinase signaling in monocytes by the Src family kinase Fgr . ^^^ Whereas the Syk tyrosine kinase is essential for beta 2 integrin signaling and cell spreading , the Src family kinase Fgr is a negative regulator of this pathway . ^^^
Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
Similar studies with macrophages lacking the Src family kinases Hck , Fgr , and Lyn showed that these kinases are not required for phagocytosis but that they enhance the rate of particle engulfment . ^^^ In this report we show that both wild type and hck ( / ) fgr ( / ) macrophages expressed Fyn , Src , and Yes and that these kinases were activated on ingestion of immunoglobulin G ( IgG ) coated particles and redistributed , together with Syk , to actin rich phagocytic cups and the phagosomal membrane . ^^^
Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
Among the Src family members , Lyn , Hck , Fgr , and Lck are present in eosinophils , and , among these four kinases , only Lyn is associated with the IL 5Ralpha under basal conditions . ^^^
Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
Platelets multiply deficient in Src , Hck , Fgr , and Lyn , or normal platelets treated with Src kinase inhibitors failed to spread on fibrinogen . ^^^
Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
Using a mouse model of allergic lung inflammation , we found that mice deficient of Fgr , a Src family tyrosine kinase highly expressed in myelomonocytic cells , fail to develop lung eosinophilia in response to repeated challenge with aerosolized OVA . ^^^ Both tissue and airway eosinophilia were markedly reduced in fgr ( / ) mice , whereas mice with the sole deficiency of Hck , another Src family member , responded normally . ^^^
Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
Using Sf 9 insect cells , we demonstrate direct STAT 3 tyrosine phosphorylation and stimulation of DNA binding activity by five members of the Src kinase family ( Src , Hck , Lyn , Fyn , and Fgr ) . ^^^
Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
Stimulation with LDL reveals ( 1 ) a major role for Tyr 925 phosphorylation which surpasses the phosphorylation of the other residues , including Tyr 397 , in rate and extent , ( 2 ) alphaIIbbeta 3 independent phosphorylation of Tyr 925 and Tyr 397 , and ( 3 ) complex formation between FAK and the Src kinase Fgr but not with c Src . ^^^
Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
These events concurred with activation of Fgr and , surprisingly , also with dissociation of this Src tyrosine kinase from Cbl . ^^^ The following observations imply that Syk is such a kinase : ( 1 ) beta ( 2 ) integrins activated Syk in a Src dependent manner , ( 2 ) Syk was associated with Cbl much longer than Fgr was , and ( 3 ) the Syk inhibitor piceatannol ( 3 , 4 , 3 ' , 5 ' tetrahydroxy trans stilbene ) abolished the Cbl associated kinase activity in an in vitro assay . ^^^
Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
In this study , we further showed that the proline rich domain of DOC 2 / DAB2 could also interact with proteins containing the Src homology 3 domain , such as Src and Fgr . ^^^
Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
As Hck and Fgr expression and activity were not decreased in c cbl ( / ) macrophages , these results suggest that Cbl is likely to be an important downstream mediator of the Src family kinase regulated macrophage motility pathway . . ^^^
Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
RESULTS : Array profiles demonstrate that Fyn , Lyn , Fgr , Hck , Src , and Yes are all expressed in cultured human MKs ( Fyn , Lyn > Src , Yes , Fgr , Hck ) . ^^^ Similarly , Western blots of murine MKs identified the same six SFKs ( Fyn , Fgr , Hck , Lyn , Src , and Yes ) . ^^^
Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
One group of SH 2 domains ( Src , Fyn , Lck , Fgr , Abl , Crk , and Nck ) preferred sequences with the general motif pTyr hydrophilic hydrophilic Ile / Pro while another group ( SH 2 domains of p 85 , phospholipase C gamma , and SHPTP 2 ) selected the general motif pTyr hydrophobic 10 hydrophobic . ^^^ Individual members of these groups selected unique sequences , except the Src subfamily ( Src , Fyn , Lck , and Fgr ) , which all selected the sequence pTyr Glu Glu Ile . ^^^
Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
In this study , we demonstrate that mice deficient in the src family kinases , Hck and Fgr ( hck ( / ) fgr ( / ) ) , are unable to develop an angiogenic response to CXCL1 / MIP 2 , although they respond normally to vascular endothelial growth factor A ( VEGF A ) . ^^^
Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
Requirement of Src kinases Lyn , Hck and Fgr for BCR ABL 1 induced B lymphoblastic leukemia but not chronic myeloid leukemia . ^^^ Here , we show that Bcr Abl activated the Src kinases Lyn , Hck and Fgr in B lymphoid cells . ^^^
Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
As reported here , PLD 2 but not PLD 1 is phosphorylated through the Src kinases , Fyn and Fgr , and this phosphorylation appears to regulate PLD 2 activation and degranulation . ^^^ This phosphorylation was blocked by a Src kinase inhibitor or by small interfering RNAs directed against Fyn and Fgr and was enhanced by overexpression of Fyn and Fgr but not by other Src kinases . ^^^
Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
Moreover , phosphorylated but not native apoER 2 ' co precipitated with the Src family member Fgr . ^^^
Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
LPS activated the src like kinases p59hck ( HCK ) and p58fgr ( FGR ) in both adult and newborn PMNs but increased the activation of p53 / 56lyn ( LYN ) only in adult cells . ^^^
Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
The Src family kinases Hck and Fgr negatively regulate neutrophil and dendritic cell chemokine signaling via PIR B . ^^^ These data support a model in which the Src family kinases Hck and Fgr function as negative regulators of myeloid cell chemokine signaling by maintaining the tonic phosphorylation of PIR B . . ^^^
Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
Yes kinase , but not c Src , Fgr , Hck , or Lyn , was found to exhibit up regulated kinase activity after LTB 4 stimulation . ^^^
Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
The protein expression levels of a tyrosine kinase , Fgr , were reduced in Csk knockdown cells , suggesting that Csk is a critical regulator of TLR 4 mediated signalling by modifying the levels of Src like kinases . . ^^^
Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
Protein phosphatase 1alpha is tyrosine phosphorylated and inactivated by peroxynitrite in erythrocytes through the src family kinase fgr . ^^^ We have previously shown in mouse red cells that upregulation of the src kinase fgr phosphorylates PP1alpha , acting as an upstream negative regulator of PP1alpha , and downregulates K Cl cotransport . ^^^
Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
Both EAT 2A and EAT 2B bind to the Src like kinases Fyn , Hck , Lyn , Lck , and Fgr , as determined by a yeast two hybrid assay . ^^^
Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
Dual function for the adaptor MIST in IFN gamma production by NK and CD4+NKT cells regulated by the Src kinase Fgr . ^^^ These opposite effects of MIST paralleled the exclusive expression of the Src family kinase , Fgr , in NK cells between the 2 cell populations . ^^^
Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
The Src family kinases Hck and Fgr are dispensable for inside out , chemoattractant induced signaling regulating beta 2 integrin affinity and valency in neutrophils , but are required for beta 2 integrin mediated outside in signaling involved in sustained adhesion . ^^^
Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
We expressed Fgr , Fyn , Hck , Lck , Lyn , and Yes as well as c Src in yeast and found that each kinase was active and induced growth suppression . ^^^ Nef strongly activated Hck , Lyn , and c Src but did not detectably affect Fgr , Fyn , Lck , or Yes . ^^^
Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
First , we found the src protein tyrosine kinases p53 / 56lyn , p59fyn , p56 / 59hck , p55c fgr , and p60c src to be expressed in Langerhans cells from all donors . ^^^
Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
We found that phorbol myristate acetate ( PMA ) activates the src family tyrosine kinases p58c fgr and p53 / 56lyn in suspended PMNs . ^^^
Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
Analysis of the activity of two src family tyrosine kinases , p58c fgr and p53 / 56lyn , showed that their autophosphorylating kinase activity strictly correlated with adhesion . ^^^
Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
Neither of the two reverse constructs , ROS 10 SRC 1 and ROS 10 SRC 2 , could transform CEF . ^^^ However , a transforming variant of ROS 10 SRC 2 appeared during passages of the transfected cells and was called ROS 10 SRC ( R ) . ^^^
Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
The chemical composition and microbial mutagenicity of aerosols generated by nebulizing two coal oils ( solvent refined coal [ SRC ] 1 process solvent [ PS ] and SRC 2 heavy distillate ) were found to vary with particle size . ^^^
Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
Chemical fractionation of SRC 2 heavy distillate and assay of resultant fractions allowed detection of significant transforming activity in chemical fractions , where such activity had not previously been observed . ^^^
Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
In the case of SRC 1 process solvent ( PS ) , 50 degrees F distillation cuts were obtained between 550 and 850 degrees F , while for the SRC 2 material , the 50 degrees F cuts were only obtained between 700 and 850 degrees F . ^^^
Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
Heavy distillate ( HD ) , the highest boiling coal liquid from the solvent refined coal 2 process ( SRC 2 ) , was administered by intragastric ( IG ) intubation to pregnant rats . ^^^
Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
For these studies several boiling range fractions and several solvent derived subfractions from two solvent refined coal processes ( SRC 1 and SRC 2 ) were evaluated for their ability to induce ODC . ^^^
Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
The latter material is a component fraction of an experimental oil produced in a solvent refined coal process ( SRC 2 ) pilot plant . ^^^
Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
Their mutagenic activities were compared with 2 other complex materials : a high boiling coal distillate ( The Solvent Refined Coal Process 1 ( SRC 2 ) heavy distillate ) , and a crude petroleum ( Prudhoe Bay ) . ^^^
Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
Moreover , JAK 2 also constitutively bound to a protein tyrosine phosphatase containing Src 2 regions ( SHPTP 2 ) , but JAK 1 did not . ^^^
Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
The newly recognized steroid receptor coactivators ( SRC 1 , SRC 2 , and SRC 3 ) belong to a homologous gene family and are important transcriptional mediators for nuclear receptors . ^^^ Through fluorescence in situ hybridization , we have mapped the mouse SRC 1 , SRC 2 , and SRC 3 genes to chromosomal locations 12A2 A 3 , 1A3 A 5 , and 2H2 H 4 , respectively . ^^^
Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
Differential expression and regional distribution of steroid receptor coactivators SRC 1 and SRC 2 in brain and pituitary . ^^^ Using in situ hybridization we have examined the neuroanatomical distribution of the messenger RNAs ( mRNAs ) for two functionally distinct splice variants of Steroid Receptor Coactivator 1 ( SRC 1 / NCoA 1 ) and of Steroid Receptor Coactivator 2 ( SRC 2 / NCoA 2 / GRIP 1 / TIF 2 ) . ^^^
Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
In addition , functional analysis of SRC 2 and SRC 3 coactivators indicated that the SRC family modulated PR transactivation from chromatin by a similar mechanism . . ^^^
Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
Steroid receptor coactivator 2 ( SRC 2 ) modulates the activity of the N terminal AF 1 domain . ^^^ Moreover , SRC 2 dramatically potentiates the retinoid induced RXR dependent activation of the Nur 77 ligand binding domain ( LBD ) . ^^^
Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
We examined the effects of antisense oligonucleotides to SRC 1 , SRC 2 , and SRC 3 on female sexual behavior and steroid receptor mediated transcription . ^^^ Our results reveal a significant inhibitory effect by antisense ( AS ) to SRC 1 and SRC 2 , but not SRC 3 , on hormone induced reproductive behavior . ^^^ Immunoreactive SRC 1 and SRC 2 , but not SRC 3 , proteins were abundant in the ventromedial nucleus , thus demonstrating that the biological activities of hypothalamic steroid receptors are selectively regulated by regional distribution of specific SRCs . ^^^ Collectively , the findings clearly implicate dual regulation of ERalpha dependent function by SRC 1 and SRC 2 in the intact female brain . ^^^ In the genetic , but not acute , absence of SRC 1 , up regulation of SRC 2 serves as a critical adaptive mechanism during female development . . ^^^
Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
In most brain structures where SRC 1 is expressed , SRC 2 is expressed at lower levels ; however , SRC 3 mRNA is detectable only in the hippocampus . ^^^ In the adult cerebellum , Purkinje cells ( PCs ) preferentially express SRC 1 over SRC 2 , but SRC 2 mRNA is slightly elevated in the SRC 1 ( ) / PCs . ^^^ SRC 2 is expressed in PCs after postnatal day ( P ) 10 . ^^^ The morphology and number of SRC 1 ( ) / PCs were equivalent to wild type by P 10 ; this timing correlated with the early expression of SRC 2 in the SRC 1 ( ) / PCs . ^^^
Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
However , with differentiation DRIP 205 is no longer produced , and the VDR switches partners to the SRC family ( SRC 2 and 3 ) . ^^^
Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
We then demonstrate that E 2 enhances interactions between ERalpha and the RIDs of SRC 1 and SRC 3 , whereas the interaction between ERalpha with the SRC 2 RID is ligand independent . ^^^
Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
Interestingly , the overexpression of the steroid hormone receptor coactivator 1 ( SRC 1 ) resulted in preferential transcriptional enhancement by ERbeta as well as coexpressed ERalpha and ERbeta , whereas SRC 2 overexpression appeared to preferentially enhance ERalpha transactivation . ^^^
Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
Furthermore , SRC 2 modulates the activity of the AF 1 domain but not the C terminal ligand binding domain ( LBD ) . ^^^
Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
Loss of function experiments further support the predicted roles of SRC 1 and SRC 2 in , respectively , PR and GR mediated transcription on the MMTV promoter . ^^^
Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
In particular , this article highlights the roles of SRC 1 ( NCoA 1 ) , SRC 2 ( GRIP 1 , TIF 2 , or NCoA 2 ) and SRC 3 ( p / CIP , RAC 3 , ACTR , AIB 1 , or TRAM 1 ) in development , organ function , endocrine regulation , and nuclear receptor function , which are defined by characterization of the genetically manipulated animal models . ^^^
Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
Interestingly , the phosphorylated insulin receptor interacting region ( PIR ) and Src 2 homology domains ( SH 2 ) of Grb 7 and Grb 14 were differently implicated in the inhibition of FGFR signalling . ^^^
Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
Given that Tpit exhibits transcriptional synergy with NGFI B , our results suggest that Tpit , along with NGFI B and SRC 2 , is part of a transcription regulatory complex assembled on the POMC promoter in response to hormonal stimulation . . ^^^
Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
Steroid receptor coactivator 3 ( SRC 3 / p / CIP / AIB1 / ACTR / RAC3 / TRAM 1 ) is a member of the p 160 family of nuclear receptor coactivators , which includes SRC 1 ( NCoA 1 ) and SRC 2 ( TIF2 / GRIP1 / NCoA2 ) . ^^^
Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
Amongst the coactivators cloned in Xenopus laevis , SRC 3 was found to be dramatically upregulated during natural and T 3 induced metamorphosis , and SRC 2 and p 300 are expressed throughout postembryonic development with little change in their expression levels . ^^^
Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
METHODS : Gene expression of SRC 1 , SRC 2 , SRC 3 , N CoR , SMRT , ERalpha , and PR was measured in 26 samples of normal endometrium and 30 primary endometrial carcinomas using real time RT PCR . ^^^ CONCLUSION : The nuclear receptor coregulators SRC 1 , SRC 2 , SRC 3 , N CoR , and SMRT were found to be up regulated in malignant endometrium . ^^^
Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
In mammalian two hybrid assays , DIM C pPhCF ( 3 ) and PGJ 2 ( 5 20 micro M ) induced interactions of PPARgamma with steroid receptor coactivator ( SRC ) 1 , SRC 2 ( TIFII ) , and thyroid hormone receptor associated protein 220 but not with SRC 3 ( AIB 1 ) . ^^^
Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
Using this homogenous method several coregulator NR boxes capable of associating with thyroid receptor at physiologically relevant concentrations were identified including ones found in traditional coactivating proteins such as SRC 1 , SRC 2 , TRAP 220 , TRBP , p 300 , and ARA 70 ; and those in coregulators known to repress gene activation including RIP 140 and DAX 1 . ^^^
Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
Herein we utilize a library of SRC 2 peptidomimetics to select for specific inhibitors of the interaction of SRC 2 with the two estrogen receptor ( ER ) isoforms , ERalpha and ERbeta , in the presence of three different ligands : 17beta estradiol , diethylstilbesterol , and genistein . ^^^ Several of the lead compounds are highly ( > 100 fold ) selective for blocking the binding of SRC 2 to ERalpha , in preference to ERbeta , in the presence of one ligand and therefore may prove useful for decoupling ERbeta signaling from ERalpha signaling . . ^^^
Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
The TNF alpha dependent decrease in PRE luciferase activity was fully prevented by cotransfection with SRC 2 , and partially prevented with exogenous SRC 1 . ^^^
Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
We studied the mechanisms of action of the glucocorticoid receptor ( GR ) and its interactions with p 160 coactivator family members [ steroid receptor coactivator ( SRC ) 1 ( a and e ) , SRC 2 and SRC 3 ] in mouse Schwann cells ( MSC 80 ) . ^^^ A minimal promoter containing two glucocorticoid response elements , ( GRE ) 2 TATA , recruits SRC 1 ( a and e ) and SRC 3 , whereas SRC 2 is excluded . ^^^ Within the context of the more complex mouse mammary tumor virus promoter , GR recruits SRC 1e and SRC 2 , whereas SRC 1a and SRC 3 are not implicated . ^^^
Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
CHO K 1 cells transfected with ERalpha or ERbeta show ERE sequence dependent differences in the functional interaction of ERalpha and ERbeta with coactivators steroid receptor coactivator 1 ( SRC 1 ) , SRC 2 ( glucocorticoid receptor interacting protein 1 ( GRIP 1 ) ) , SRC 3 amplified in breast cancer 1 ( AIB 1 ) and ACTR , cyclic AMP binding protein ( CBP ) , and steroid receptor RNA activator ( SRA ) , corepressors nuclear receptor co repressor ( NCoR ) and silencing mediator for retinoid and thyroid hormone receptors ( SMRT ) , and secondary coactivators coactivator associated arginine methyltransferase 1 ( CARM 1 ) and protein arginine methyltransferase 1 ( PRMT 1 ) . ^^^
Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
The present study used immunocytochemistry to identify a number of nuclear receptor coactivators that are expressed by adult lumbar motoneurons : SRC 1 , SRC 2 , CBP , p 300 , and cJUN . ^^^
Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
This study examines the expression of the co activators SRC 1a / e , SRC 2 , SRC 3 , SRA , and the corepressors NCoR and SMRT in GCT , epithelial ovarian tumours and normal ovary . ^^^ No significant difference in the expression of SRC 1 , SRC 2 , SRC 3 or NCoR and SMRT was found . ^^^
Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
The ability of SRC family coactivators to regulate the expression of one of these genes , PARD6B / Par6 , was confirmed by using cells individually depleted of SRC 1 , SRC 2 , or SRC 3 by small interfering RNA . ^^^
Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
We show that Rb and the related proteins p 107 and p 130 enhance the activity of NRs related to NGFI B ( Nur factors ) through direct interactions with NGFI B and SRC 2 . ^^^
Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
In this study , we investigated recruitment of coactivators ( SRC 1 , SRC 2 , and SRC 3 ) and corepressors ( HDAC 1 , HDAC 2 , HDAC 3 , SMRT , and NCoR ) to the IkappaB alpha gene promoter after NF kappaB activation by tumor necrosis factor alpha . ^^^ SRC 1 , SRC 2 , and SRC 3 all enhanced IkappaB alpha transcription . ^^^ After tumor necrosis factor alpha treatment , SRC 1 signal was increased gradually , but SRC 2 signal was reduced immediately , suggesting replacement of SRC 2 by SRC 1 . ^^^
Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
The aims of this study were to compare the expression of the SRC 1 , SRC 2 , and SRC 3 coactivators between fibroids and normal myometrium in pure populations of cultured smooth muscle cells ( SMC ) and microvascular endothelial cells ( MEC ) , and also between both cell types , and to identify any relationship between the SRC expression profiles and the known ER status of the SMC and MEC samples examined in this study . ^^^ METHODS : Reverse transcriptase polymerase chain reaction ( RT PCR ) coupled with Southern blot analysis was used to derive a semiquantitative estimate of the relative levels of SRC 1 , SRC 2 , and SRC 3 expression in pure populations of SMC ( > 98 % alpha smooth muscle actin [ SMA ] ( + ) ) and MEC ( > 99 % CD 31 ( + ) ) isolated and cultured from eight samples of paired human myometrial and fibroid tissue . ^^^ RESULTS : The mean levels of SRC 1 , SRC 2 , and SRC 3 were each similar in normal myometrium compared to fibroids for SMC and also for MEC . ^^^ However , SRC 1 , SRC 2 , and SRC 3 levels were each significantly higher in SMC compared to MEC from both myometrial and fibroid samples , although for SRC 3 there was a trend for higher levels in myometrial samples that did not reach significance . ^^^
Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
They exert their effects on neuronal and glial cells by means of their cognate receptor , the glucocorticoid receptor ( GR ) , which recruits the p 160 coactivator family members SRC 1 ( steroid receptor coactivator 1 ) , SRC 2 , and SRC 3 after hormone binding . ^^^ For example , within astrocytes , SRC 1 and SRC 2 were mainly nuclear , whereas SRC 3 unexpectedly localized to the lumen of the Golgi apparatus . ^^^ In contrast , in Schwann cells , SRC 1 showed a nucleocytoplasmic shuttling depending on hormonal stimulation , whereas SRC 2 remained strictly nuclear and SRC 3 remained predominantly cytoplasmic . ^^^
Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
An examination of potential compensatory mechanism ( s ) revealed an increase in adrenal weight , selective elevation of melanocortin 2 receptor mRNA , and a coincident increase in SRC 2 and SRC 3 expression in SRC 1 / adrenals . ^^^
Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
VDR and C / EBP alpha were recruited concurrently to the composite element along with the coactivators p 300 , steroid receptor coactivator 1 ( SRC 1 ) , and SRC 2 , but not SRC 3 . ^^^
Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
Three steroid receptor coactivators ( SRC ) 1 , SRC 2 , and SRC 3 were expressed . ^^^
Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
Members of the steroid receptor coactivator ( SRC ) family , which include SRC 1 ( NcoA 1 / p160 ) , SRC 2 ( TIF2 / GRIP1 / NcoA 2 ) and SRC 3 ( pCIP / RAC3 / ACTR / pCIP / AIB1 / TRAM1 ) , are critical mediators of steroid receptor action . ^^^ Gene ablation studies previously identified SRC 1 and SRC 2 as being involved in the control of energy homeostasis . ^^^ A genomic approach using microarray analysis was employed to identify the subsets of genes that are altered in the livers of SRC 1 / , SRC 2 / , and SRC 3 / mice . ^^^ The overall pattern of altered gene expressions in the SRC 1 / mice was up regulation , whereas SRC 2 / mice showed an overall down regulation . ^^^ Several key regulatory enzymes of energy metabolism were significantly altered in the liver of SRC 2 / mice , which are consistent with the prior observation that SRC 2 / mice have increased energy expenditure . ^^^
Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
To further dissect the roles of members of the steroid receptor coactivator ( SRC ) / p160 family in PR mediated reproductive processes in vivo , state of the art cre loxP engineering strategies were employed to generate a mouse model ( PR ( Cre / + ) SRC 2 ( flox / flox ) ) in which SRC 2 function was abrogated only in cell lineages that express the PR . ^^^ Fertility tests revealed that while ovarian activity was normal , PR ( Cre / + ) SRC 2 ( flox / flox ) mouse uterine function was severely compromised . ^^^ Absence of SRC 2 in PR positive uterine cells was shown to contribute to an early block in embryo implantation , a phenotype not shared by SRC 1 or 3 knockout mice . ^^^ In addition , histological and molecular analyses revealed an inability of the PR ( Cre / + ) SRC 2 ( flox / flox ) mouse uterus to undergo the necessary cellular and molecular changes that precede complete P induced decidual progression . ^^^ Moreover , removal of SRC 1 in the PR ( Cre / + ) SRC 2 ( flox / flox ) mouse uterus resulted in the absence of a decidual response , confirming that uterine SRC 2 and 1 cooperate in P initiated transcriptional programs which lead to full decidualization . ^^^
Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
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Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
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Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
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Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
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Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
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Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
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Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
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Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
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Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
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Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
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Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
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Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
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Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
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Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
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Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
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Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
NA
Interacting proteins: P09769 and P12931	Pubmed	SVM Score :0.0
NA