| Interacting proteins: P09603 and P12931 |
Pubmed |
SVM Score :0.0 |
| There was no increase in the secretion of either CSF 1 or IL 6 by either S17 / v src or S17 / c src . ^^^ |
|
| Interacting proteins: P09603 and P12931 |
Pubmed |
SVM Score :0.0 |
| When bone marrow derived monocytic cells were synchronized and stimulated to proliferate with CSF 1 , c fgr transcripts ( but not transcripts from the highly related genes c src or c yes ) were induced 8 hr after the addition of CSF 1 and decreased to low levels by 20 hr as the monocytic cells entered S phase . ^^^ |
|
| Interacting proteins: P09603 and P12931 |
Pubmed |
SVM Score :0.0 |
| We show that CSF 1 stimulation resulted in activation of three Src family kinases , Src , Fyn and Yes . ^^^ Concomitant with their activation , all three Src family kinases were found to associate with the ligand activated CSF 1 receptor . ^^^ Because fibroblasts expressing this receptor mutant are unable to form colonies in semi solid medium or to grow in chemically defined medium in the presence of CSF 1 , the Src family kinases may play a physiological role in the mitogenic response to CSF 1 . . ^^^ |
|
| Interacting proteins: P09603 and P12931 |
Pubmed |
SVM Score :0.0 |
| The activated colony stimulating factor 1 ( CSF 1 ) receptor , which is known to associate with Src family kinases , has a sequence in its juxtamembrane region similar to that surrounding Tyr 579 of the PDGF receptor , and a phosphopeptide modeled on this sequence competed the association of Fyn with the receptor in vitro . ^^^ Furthermore , mutational analysis demonstrated that these sequences were required for the efficient association of Src family kinases with the activated CSF 1 receptor in vivo . ^^^ Phosphopeptides corresponding to the Src family binding sites of both PDGF and CSF 1 receptors activated Src kinase activity in vitro . ^^^ |
|
| Interacting proteins: P09603 and P12931 |
Pubmed |
SVM Score :0.0 |
| Tyrosine phosphorylation of c Cbl occurred within 20 s after stimulation and reached maximum levels within 3 5 min . c Cbl was also tyrosine phosphorylated in epidermal growth factor ( EGF ) receptor overexpressing cells upon EGF stimulation , in macrophages in response to CSF 1 treatment , and in 5 src transformed cells . ^^^ |
|
| Interacting proteins: P09603 and P12931 |
Pubmed |
SVM Score :0.0 |
| Microinjection of this antibody was used to demonstrate that Src kinases were required for both CSF 1 and EGF to drive cells into the S phase . ^^^ Expression of a kinase inactive form of Src family kinases also prevented EGF and CSF 1 stimulated DNA synthesis . ^^^ |
|
| Interacting proteins: P09603 and P12931 |
Pubmed |
SVM Score :0.0 |
| P 130 and P 75 may be direct substrates of c fps / fes : P 130 was tightly associated with NCP 92 , and the src homology 2 domain of NCP 92 specifically bound phosphorylated P 130 and P 75 but not the CSF 1 induced phosphotyrosyl proteins , consistent with the possibility that P 130 and P 75 are physiological targets of c fps / fes . ^^^ |
|
| Interacting proteins: P09603 and P12931 |
Pubmed |
SVM Score :0.0 |
| Recent studies have shown that the enzyme phosphatidylinositol 3 kinase ( PI 3 kinase ) binds tightly to the activated platelet derived growth factor ( PDGF ) and colony stimulating factor 1 ( CSF 1 ) receptors , through interaction of the src homology 2 ( SH 2 ) domains on the 85 kD subunit of PI 3 kinase with Ptyr in one of these motifs on the receptors . ^^^ |
|
| Interacting proteins: P09603 and P12931 |
Pubmed |
SVM Score :0.0 |
| Stat 3 is also activated by CSF 1 , possibly through CSF 1 receptor c Src association in NIH 3T3 cells overexpressing CSF 1 receptors . ^^^ |
|
| Interacting proteins: P09603 and P12931 |
Pubmed |
SVM Score :0.0 |
| The novel catenin p120cas binds classical cadherins and induces an unusual morphological phenotype in NIH3T3 fibroblasts . p120cas ( CAS ) is a tyrosine kinase substrate whose phosphorylation has been implicated in cell transformation by Src and in ligand induced signaling through the EGF , PDGF , and CSF 1 receptors . ^^^ |
|
| Interacting proteins: P09603 and P12931 |
Pubmed |
SVM Score :0.0 |
| It has been reported previously that c Src becomes tyrosine phosphorylated following CSF 1 treatment of fibroblasts overexpressing c Fms . ^^^ We therefore examined the cellular events associated with CSF 1 induced spreading in osteoclasts and what role , if any , c Src played in these processes . ^^^ The c Src kinase activity was increased 2 . 9 fold following CSF 1 treatment . ^^^ We conclude that CSF 1 induced osteoclast spreading is accompanied by rapid reorganization of the actin cytoskeleton and phosphorylation of several cellular substrates , including c Fms and c Src . ^^^ In contrast , CSF 1 failed to induce any cytoplasmic spreading in osteoclasts isolated from mice with targeted disruption of the src gene . ^^^ |
|
| Interacting proteins: P09603 and P12931 |
Pubmed |
SVM Score :0.0 |
| Cellular Src ( c Src ) , prototype for a family of intracellular membrane associated tyrosine kinases , is required for mitogenesis initiated by multiple growth factor receptors , including the receptors for epidermal growth factor ( EGF ) , platelet derived growth factor ( PDGF ) , colony stimulating factor 1 ( CSF 1 ) , and the basic fibroblast growth factor ( bFGF ) . ^^^ |
|
| Interacting proteins: P09603 and P12931 |
Pubmed |
SVM Score :0.0 |
| CSF 1 increases membrane phosphotyrosine staining in osteoclasts and induces the phosphorylation of several cellular proteins in cultured , osteoclast like cells , including c fms , c src , and an 85 kD Grb 2 binding protein . ^^^ These results indicate that c src is critical for the normal cytoskeletal architecture of the osteoclast , and , in its absence , the spreading response induced by CSF 1 is abrogated , and downstream signaling from c fms is altered . . ^^^ Src kinase activity is increased threefold after CSF 1 treatment . ^^^ In src cells , no attachment ring is present , and CSF 1 fails to induce spreading or a change in the pattern of F actin distribution . ^^^ Although c fms becomes phosphorylated after CSF 1 treatment , the 85 kD protein is significantly less phosphorylated in src osteoclast like cells . ^^^ |
|
| Interacting proteins: P09603 and P12931 |
Pubmed |
SVM Score :0.0 |
| Herein we demonstrate that CSF 1 induced osteoclast spreading depends on the substrate the osteoclast interacts with and requires integrity of the vitronectin receptor and of the c src proto oncogene . ^^^ To establish involvement of the vitronectin receptor and c src proto oncogene , cells were treated with the alpha vbeta 3 integrin neutralizing antibody , LM 609 , or c src antisense oligonucleotides , which reduced CSF 1 induced osteoclast spreading by 57 % and 60 % , respectively . ^^^ The results demonstrate that CSF 1 induced osteoclast spreading requires both the vitronectin receptor and the c src proto oncogene and that this action is modulated by the adhesion substrata . . ^^^ |
|
| Interacting proteins: P09603 and P12931 |
Pubmed |
SVM Score :0.0 |
| It was originally identified as a prominent Src substrate and subsequently as a substrate for the Platelet Derived Growth Factor ( PDGF ) , Epidermal Growth Factor ( EGF ) , and Colony Stimulating Factor 1 ( CSF 1 ) receptor tyrosine kinases . ^^^ |
|
| Interacting proteins: P09603 and P12931 |
Pubmed |
SVM Score :0.0 |
| Using microinjection to introduce catalytically inactive dominant negative form of cSrc , as well as an antibody that neutralizes cSrc , Fyn and cYes , we have shown that Src kinases are required for DNA synthesis induced by most growth factors ( PDGF , EGF , CSF 1 , insulin , IGF 1 ) . ^^^ |
|
| Interacting proteins: P09603 and P12931 |
Pubmed |
SVM Score :0.0 |
| A small pool of CSF 1R formed a multimeric complex with phosphatidylinositol 3 kinase ( PI 3 kinase ) , SHP 1 , Grb 2 , Shc , c Src , Cbl , and a significant number of tyrosine phosphorylated proteins in CSF 1 stimulated cells . ^^^ |
|
| Interacting proteins: P09603 and P12931 |
Pubmed |
SVM Score :0.0 |
| The Src tyrosine kinases c Src , c Yes , c Fyn , and c Hck were tyrosine phosphorylated in the M1 / WT cells in response to CSF 1 and bound to the WT CSF 1R through their SH 2 domains . ^^^ Expression of a Y559F mutant CSF 1 receptor in M 1 myeloid cells : a role for Src kinases in CSF 1 receptor mediated differentiation . ^^^ Binding of the Src kinases to the CSF 1 receptor was greatly reduced in the M1 / Y559F cells . ^^^ Treatment of M1 / WT cells with the Src family inhibitor PP 2 resulted in an inhibition of CSF 1 mediated differentiation , equivalent to that observed in the M1 / Y559F cells . ^^^ |
|
| Interacting proteins: P09603 and P12931 |
Pubmed |
SVM Score :0.0 |
| The growth factor / cytokine , colony stimulating factor 1 ( CSF 1 ) , potently stimulates osteoclast motility , in a c src dependent fashion . ^^^ The intracellular signaling molecules that participate with c src in CSF 1 induced remodeling of the osteoclast cytoskeleton have not been identified . ^^^ After CSF 1 treatment of osteoclast like cells , PI 3 K activity associated with the CSF 1 receptor c fms , is increased , and the 85 kDa regulatory subunit of PI 3 K and c src coimmunoprecipitate . ^^^ Taken together , the current data suggest that c src , via its SH 3 domain , may participate in CSF 1 induced activation of PI 3 K and that PI 3 K and c src are in the signaling pathway that subserves CSF 1 induced cytoskeletal changes in osteoclasts . . ^^^ |
|
| Interacting proteins: P09603 and P12931 |
Pubmed |
SVM Score :0.0 |
| To determine if Src family kinases ( SFKs ) are involved , we demonstrated that CSF 1 activated Fyn and Lyn in cells expressing wild type ( WT ) or DeltaKI receptors . ^^^ |
|
| Interacting proteins: P09603 and P12931 |
Pubmed |
SVM Score :0.0 |
| Finally , simultaneous inhibition of Src , MEK activation , and c myc expression additively blocks CSF 1 induced D 2 expression . ^^^ |
|
| Interacting proteins: P09603 and P12931 |
Pubmed |
SVM Score :0.0 |
| Additionally , autophosphorylated Tyr 559 in the JMD of the colony stimulating factor 1 ( CSF 1 ) receptor ( CSF 1R ) binds to Src family kinases ( SFKs ) . ^^^ |
|
| Interacting proteins: P09603 and P12931 |
Pubmed |
SVM Score :0.0 |
| Growth factors that influence many Src family proteins include the PDGF R , CSF 1 receptor and others . ^^^ |
|
| Interacting proteins: P09603 and P12931 |
Pubmed |
SVM Score :0.0 |
| Noncontact HUBEC cultures and the addition of thrombopoietin , stem cell factor ( SCF ) , and macrophage colony stimulating factor 1 receptor ( Fms ) like tyrosine kinase 3 ( Flt 3 ) ligand supported further increases in CD 34 ( + ) CD 38 cells ( 6 . 4 fold and 13 . 1 fold ) , which correlated with significant increases in SRC activity . ^^^ |
|
| Interacting proteins: P09603 and P12931 |
Pubmed |
SVM Score :0.0 |
| Furthermore , ubenimex augmented the expression of M CSF receptors by U 937 cells and enhanced the tyrosine kinase activity of cellular pp60c src . ^^^ |
|
| Interacting proteins: P09603 and P12931 |
Pubmed |
SVM Score :0.0 |
| The effect of GM CSF and M CSF on the activation of fyn kinase , a 59 kDa src family related protein tyrosine kinase ( PTK ) , was studied . ^^^ |
|
| Interacting proteins: P09603 and P12931 |
Pubmed |
SVM Score :0.0 |
| The expression of c src , c fms , and macrophage colony stimulating factor ( M CSF ) was induced by TPA treatment ; however , TPA induced M CSF gene transcription was attenuated by the subsequent addition of 1 , 25 ( OH ) 2D3 . ^^^ |
|
| Interacting proteins: P09603 and P12931 |
Pubmed |
SVM Score :0.0 |
| Some ubiquitously expressed genes seem unexpectedly to have unique functions that are largely confined to bone cells : M CSF , C Fos , PU . 1 , and NF kappaB are required for osteoclast formation , whereas c Src and Mitf ( microphthalmia transcription factor ) are required for osteoclast activity after the cells have formed . ^^^ |
|
| Interacting proteins: P09603 and P12931 |
Pubmed |
SVM Score :0.0 |
| In the human monocytic cell line , THP 1 , SHIP 1 became tyrosine phosphorylated following M CSF activation in a Src family kinase dependent manner . ^^^ |
|
| Interacting proteins: P09603 and P12931 |
Pubmed |
SVM Score :0.0 |
| These results suggest that in osteoclasts 1 ) tyrosine phosphorylation of p130Cas depends on alpha 5 beta 3 integrin mediated cell adhesion , even in the presence of M CSF ; 2 ) on the other hand , c Cbl phosphorylation is predominantly activated by M CSF and is independent of cell adhesion ; 3 ) lastly , although c Src is essential for both adhesion and M CSF mediated phosphorylation of p130Cas , it is clearly not required for c Cbl phosphorylation in M CSF treated pOCs . ^^^ The purpose of this study was to examine adhesion and macrophage colony stimulating factor ( M CSF ) induced tyrosine phosphorylation of these two molecules in prefusion osteoclasts ( pOCs ) derived from either Src+ / . or Src / mice and to directly compare the roles of p130Cas and c Cbl in osteoclast function . ^^^ In Src deficient pOCs plated on vitronectin , although M CSF highly induces Cbl phosphorylation , it does not affect p130Cas phosphorylation . ^^^ |
|
| Interacting proteins: P09603 and P12931 |
Pubmed |
SVM Score :0.0 |
| We report that 1 ) SHIP 2 was tyrosine phosphorylated in M CSF stimulated human alveolar macrophages , human THP 1 cells , murine macrophages , and the murine macrophage cell line RAW 264 ; 2 ) SHIP 2 associated with the M CSF receptor after M CSF stimulation ; and 3 ) SHIP 2 associated with the actin binding protein filamin and localization to the cell membrane , requiring the proline rich domain , but not on the Src homology 2 domain of SHIP 2 . ^^^ Analyzing the function of SHIP 2 in M CSF stimulated cells by expressing either wild type SHIP 2 or an Src homology 2 domain mutant of SHIP 2 reduced Akt activation in response to M CSF stimulation . ^^^ |
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| Interacting proteins: P09603 and P12931 |
Pubmed |
SVM Score :0.0 |
| The induction of M CSF was blocked by a phosphatidylinositol 3 kinase ( PI 3 kinase ) inhibitor ( LY 294002 ) , a Src family tyrosine kinase inhibitor ( PP 1 ) and an Akt inhibitor . ^^^ |
|
| Interacting proteins: P09603 and P12931 |
Pubmed |
SVM Score :0.0 |
| These results indicate that , upon M CSF stimulation , PLC gamma 2 cellular localisation and phosphorylation are strongly dependent on cytoskeleton architecture of the macrophage precursor as well as the PI 3 kinase and the Src kinases . . ^^^ |
|
| Interacting proteins: P09603 and P12931 |
Pubmed |
SVM Score :0.0 |
| Using a panel of cytoplasmic domain mutants of the chimeric M CSF / PDGFR beta , we confirmed that the kinase domain of PDGFR beta is absolutely required for LRP tyrosine phosphorylation but that PDGFR beta mediated activation of phosphatidylinositol 3 kinase , RasGAP , SHP 2 , phospholipase C gamma , and Src are not necessary for LRP tyrosine phosphorylation . ^^^ |
|
| Interacting proteins: P09603 and P12931 |
Pubmed |
SVM Score :0.0 |
| Following M CSF treatment , FAK and the active forms of M CSFR and Src were redistributed to the cytoskeleton , where active ERK , JNK and PI3K were detectable . ^^^ |
|
| Interacting proteins: P09603 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P09603 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P09603 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P09603 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P09603 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P09603 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P09603 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P09603 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P09603 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P09603 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P09603 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P09603 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P09603 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P09603 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P09603 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P09603 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P09603 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P09603 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P09603 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P09603 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P09603 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P09603 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P09603 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P09603 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P09603 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P09603 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
|